Id |
Subject |
Object |
Predicate |
Lexical cue |
T10169 |
10-13 |
DT |
denotes |
The |
T10170 |
25-33 |
NN |
denotes |
Ortholog |
T10171 |
14-19 |
NN |
denotes |
Mouse |
T10172 |
20-24 |
NN |
denotes |
PCH2 |
T10173 |
34-40 |
NN |
denotes |
TRIP13 |
T10174 |
41-44 |
CC |
denotes |
and |
T10175 |
45-55 |
NN |
denotes |
Expression |
T10176 |
56-58 |
IN |
denotes |
in |
T10177 |
59-63 |
JJ |
denotes |
Wild |
T10178 |
64-68 |
NN |
denotes |
Type |
T10179 |
69-72 |
CC |
denotes |
and |
T10180 |
73-79 |
NN |
denotes |
Mutant |
T10181 |
79-136 |
sentence |
denotes |
(A) Phylogenetic tree of presumed PCH2/TRIP13 orthologs. |
T10182 |
80-81 |
-LRB- |
denotes |
( |
T10183 |
81-82 |
LS |
denotes |
A |
T10184 |
97-101 |
NN |
denotes |
tree |
T10185 |
82-83 |
-RRB- |
denotes |
) |
T10186 |
84-96 |
JJ |
denotes |
Phylogenetic |
T10187 |
102-104 |
IN |
denotes |
of |
T10188 |
105-113 |
JJ |
denotes |
presumed |
T10189 |
126-135 |
NNS |
denotes |
orthologs |
T10190 |
114-118 |
NN |
denotes |
PCH2 |
T10191 |
119-125 |
NN |
denotes |
TRIP13 |
T10192 |
118-119 |
HYPH |
denotes |
/ |
T10193 |
135-136 |
. |
denotes |
. |
T10194 |
136-217 |
sentence |
denotes |
The database sequence accession number of each protein is presented in Table S1. |
T10195 |
137-140 |
DT |
denotes |
The |
T10196 |
169-175 |
NN |
denotes |
number |
T10197 |
141-149 |
NN |
denotes |
database |
T10198 |
150-158 |
NN |
denotes |
sequence |
T10199 |
159-168 |
NN |
denotes |
accession |
T10200 |
195-204 |
VBN |
denotes |
presented |
T10201 |
176-178 |
IN |
denotes |
of |
T10202 |
179-183 |
DT |
denotes |
each |
T10203 |
184-191 |
NN |
denotes |
protein |
T10204 |
192-194 |
VBZ |
denotes |
is |
T10205 |
205-207 |
IN |
denotes |
in |
T10206 |
208-213 |
NN |
denotes |
Table |
T10207 |
214-216 |
NN |
denotes |
S1 |
T10208 |
216-217 |
. |
denotes |
. |
T10209 |
217-281 |
sentence |
denotes |
Numbers shown are bootstrap values (see Materials and Methods). |
T10210 |
218-225 |
NNS |
denotes |
Numbers |
T10211 |
232-235 |
VBP |
denotes |
are |
T10212 |
226-231 |
VBN |
denotes |
shown |
T10213 |
236-245 |
NN |
denotes |
bootstrap |
T10214 |
246-252 |
NNS |
denotes |
values |
T10215 |
253-254 |
-LRB- |
denotes |
( |
T10216 |
254-257 |
VB |
denotes |
see |
T10217 |
258-267 |
NNS |
denotes |
Materials |
T10218 |
268-271 |
CC |
denotes |
and |
T10219 |
272-279 |
NNS |
denotes |
Methods |
T10220 |
279-280 |
-RRB- |
denotes |
) |
T10221 |
280-281 |
. |
denotes |
. |
T10222 |
281-466 |
sentence |
denotes |
The clustering was the same regardless of whether the whole entire AA sequence or trimmed sequences (where regions showing little conservation were removed) were used for the analysis. |
T10223 |
282-285 |
DT |
denotes |
The |
T10224 |
286-296 |
NN |
denotes |
clustering |
T10225 |
297-300 |
VBD |
denotes |
was |
T10226 |
301-304 |
DT |
denotes |
the |
T10227 |
305-309 |
JJ |
denotes |
same |
T10228 |
310-320 |
RB |
denotes |
regardless |
T10229 |
321-323 |
IN |
denotes |
of |
T10230 |
324-331 |
IN |
denotes |
whether |
T10231 |
444-448 |
VBN |
denotes |
used |
T10232 |
332-335 |
DT |
denotes |
the |
T10233 |
352-360 |
NN |
denotes |
sequence |
T10234 |
336-341 |
JJ |
denotes |
whole |
T10235 |
342-348 |
JJ |
denotes |
entire |
T10236 |
349-351 |
NN |
denotes |
AA |
T10237 |
361-363 |
CC |
denotes |
or |
T10238 |
364-371 |
VBN |
denotes |
trimmed |
T10239 |
372-381 |
NNS |
denotes |
sequences |
T10240 |
382-383 |
-LRB- |
denotes |
( |
T10241 |
383-388 |
WRB |
denotes |
where |
T10242 |
430-437 |
VBN |
denotes |
removed |
T10243 |
389-396 |
NNS |
denotes |
regions |
T10244 |
397-404 |
VBG |
denotes |
showing |
T10245 |
405-411 |
JJ |
denotes |
little |
T10246 |
412-424 |
NN |
denotes |
conservation |
T10247 |
425-429 |
VBD |
denotes |
were |
T10248 |
437-438 |
-RRB- |
denotes |
) |
T10249 |
439-443 |
VBD |
denotes |
were |
T10250 |
449-452 |
IN |
denotes |
for |
T10251 |
453-456 |
DT |
denotes |
the |
T10252 |
457-465 |
NN |
denotes |
analysis |
T10253 |
465-466 |
. |
denotes |
. |
T10254 |
466-603 |
sentence |
denotes |
Major eukaryotic groups are indicated in color, with deuterostomia in blue, plants in green, protostomia in purple, and fungi in maroon. |
T10255 |
467-472 |
JJ |
denotes |
Major |
T10256 |
484-490 |
NNS |
denotes |
groups |
T10257 |
473-483 |
JJ |
denotes |
eukaryotic |
T10258 |
495-504 |
VBN |
denotes |
indicated |
T10259 |
491-494 |
VBP |
denotes |
are |
T10260 |
505-507 |
IN |
denotes |
in |
T10261 |
508-513 |
NN |
denotes |
color |
T10262 |
513-515 |
, |
denotes |
, |
T10263 |
515-519 |
IN |
denotes |
with |
T10264 |
534-536 |
IN |
denotes |
in |
T10265 |
520-533 |
NN |
denotes |
deuterostomia |
T10266 |
537-541 |
NN |
denotes |
blue |
T10267 |
541-543 |
, |
denotes |
, |
T10268 |
543-549 |
NNS |
denotes |
plants |
T10269 |
550-552 |
IN |
denotes |
in |
T10270 |
553-558 |
NN |
denotes |
green |
T10271 |
558-560 |
, |
denotes |
, |
T10272 |
560-571 |
NN |
denotes |
protostomia |
T10273 |
572-574 |
IN |
denotes |
in |
T10274 |
575-581 |
NN |
denotes |
purple |
T10275 |
581-583 |
, |
denotes |
, |
T10276 |
583-586 |
CC |
denotes |
and |
T10277 |
587-592 |
NNS |
denotes |
fungi |
T10278 |
593-595 |
IN |
denotes |
in |
T10279 |
596-602 |
NN |
denotes |
maroon |
T10280 |
602-603 |
. |
denotes |
. |
T10281 |
603-824 |
sentence |
denotes |
(B) Amplification products of cDNA from the following tissues: 1, heart; 2, brain; 3, spleen; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; 8, testis; 9, E7 embryo; 10, E11 embryo; 11, E15 embryo; and 12, E17 embryo. |
T10282 |
604-605 |
-LRB- |
denotes |
( |
T10283 |
605-606 |
LS |
denotes |
B |
T10284 |
622-630 |
NNS |
denotes |
products |
T10285 |
606-607 |
-RRB- |
denotes |
) |
T10286 |
608-621 |
NN |
denotes |
Amplification |
T10287 |
631-633 |
IN |
denotes |
of |
T10288 |
634-638 |
NN |
denotes |
cDNA |
T10289 |
639-643 |
IN |
denotes |
from |
T10290 |
644-647 |
DT |
denotes |
the |
T10291 |
658-665 |
NNS |
denotes |
tissues |
T10292 |
648-657 |
JJ |
denotes |
following |
T10293 |
665-667 |
: |
denotes |
: |
T10294 |
667-668 |
LS |
denotes |
1 |
T10295 |
670-675 |
NN |
denotes |
heart |
T10296 |
668-670 |
, |
denotes |
, |
T10297 |
675-676 |
: |
denotes |
; |
T10298 |
677-678 |
LS |
denotes |
2 |
T10299 |
680-685 |
NN |
denotes |
brain |
T10300 |
678-680 |
, |
denotes |
, |
T10301 |
685-686 |
: |
denotes |
; |
T10302 |
687-688 |
LS |
denotes |
3 |
T10303 |
690-696 |
NN |
denotes |
spleen |
T10304 |
688-690 |
, |
denotes |
, |
T10305 |
696-697 |
: |
denotes |
; |
T10306 |
698-699 |
LS |
denotes |
4 |
T10307 |
701-705 |
NN |
denotes |
lung |
T10308 |
699-701 |
, |
denotes |
, |
T10309 |
705-706 |
: |
denotes |
; |
T10310 |
707-708 |
LS |
denotes |
5 |
T10311 |
710-715 |
NN |
denotes |
liver |
T10312 |
708-710 |
, |
denotes |
, |
T10313 |
715-716 |
: |
denotes |
; |
T10314 |
717-718 |
LS |
denotes |
6 |
T10315 |
729-735 |
NN |
denotes |
muscle |
T10316 |
718-720 |
, |
denotes |
, |
T10317 |
720-728 |
JJ |
denotes |
skeletal |
T10318 |
735-736 |
: |
denotes |
; |
T10319 |
737-738 |
LS |
denotes |
7 |
T10320 |
740-746 |
NN |
denotes |
kidney |
T10321 |
738-740 |
, |
denotes |
, |
T10322 |
746-747 |
: |
denotes |
; |
T10323 |
748-749 |
LS |
denotes |
8 |
T10324 |
751-757 |
NN |
denotes |
testis |
T10325 |
749-751 |
, |
denotes |
, |
T10326 |
757-758 |
: |
denotes |
; |
T10327 |
759-760 |
LS |
denotes |
9 |
T10328 |
765-771 |
NN |
denotes |
embryo |
T10329 |
760-762 |
, |
denotes |
, |
T10330 |
762-764 |
NN |
denotes |
E7 |
T10331 |
771-772 |
: |
denotes |
; |
T10332 |
773-775 |
LS |
denotes |
10 |
T10333 |
781-787 |
NN |
denotes |
embryo |
T10334 |
775-777 |
, |
denotes |
, |
T10335 |
777-780 |
NN |
denotes |
E11 |
T10336 |
787-788 |
: |
denotes |
; |
T10337 |
789-791 |
LS |
denotes |
11 |
T10338 |
797-803 |
NN |
denotes |
embryo |
T10339 |
791-793 |
, |
denotes |
, |
T10340 |
793-796 |
NN |
denotes |
E15 |
T10341 |
803-804 |
: |
denotes |
; |
T10342 |
805-808 |
CC |
denotes |
and |
T10343 |
809-811 |
LS |
denotes |
12 |
T10344 |
817-823 |
NN |
denotes |
embryo |
T10345 |
811-813 |
, |
denotes |
, |
T10346 |
813-816 |
NN |
denotes |
E17 |
T10347 |
823-824 |
. |
denotes |
. |
T10348 |
824-900 |
sentence |
denotes |
(C) Intron–exon structure of TRIP13 and insertion site of gene-trap vector. |
T10349 |
825-826 |
-LRB- |
denotes |
( |
T10350 |
826-827 |
LS |
denotes |
C |
T10351 |
841-850 |
NN |
denotes |
structure |
T10352 |
827-828 |
-RRB- |
denotes |
) |
T10353 |
829-835 |
NN |
denotes |
Intron |
T10354 |
836-840 |
NN |
denotes |
exon |
T10355 |
835-836 |
HYPH |
denotes |
– |
T10356 |
851-853 |
IN |
denotes |
of |
T10357 |
854-860 |
NN |
denotes |
TRIP13 |
T10358 |
861-864 |
CC |
denotes |
and |
T10359 |
865-874 |
NN |
denotes |
insertion |
T10360 |
875-879 |
NN |
denotes |
site |
T10361 |
880-882 |
IN |
denotes |
of |
T10362 |
883-887 |
NN |
denotes |
gene |
T10363 |
888-892 |
NN |
denotes |
trap |
T10364 |
887-888 |
HYPH |
denotes |
- |
T10365 |
893-899 |
NN |
denotes |
vector |
T10366 |
899-900 |
. |
denotes |
. |
T10367 |
900-988 |
sentence |
denotes |
See Materials and Methods for details on how the precise insertion site was identified. |
T10368 |
901-904 |
VB |
denotes |
See |
T10369 |
905-914 |
NNS |
denotes |
Materials |
T10370 |
915-918 |
CC |
denotes |
and |
T10371 |
919-926 |
NNS |
denotes |
Methods |
T10372 |
927-930 |
IN |
denotes |
for |
T10373 |
931-938 |
NNS |
denotes |
details |
T10374 |
939-941 |
IN |
denotes |
on |
T10375 |
942-945 |
WRB |
denotes |
how |
T10376 |
977-987 |
VBN |
denotes |
identified |
T10377 |
946-949 |
DT |
denotes |
the |
T10378 |
968-972 |
NN |
denotes |
site |
T10379 |
950-957 |
JJ |
denotes |
precise |
T10380 |
958-967 |
NN |
denotes |
insertion |
T10381 |
973-976 |
VBD |
denotes |
was |
T10382 |
987-988 |
. |
denotes |
. |
T10383 |
988-1051 |
sentence |
denotes |
(D) RT-PCR of Trip13 and a control gene Med31 from testis RNA. |
T10384 |
989-990 |
-LRB- |
denotes |
( |
T10385 |
990-991 |
LS |
denotes |
D |
T10386 |
996-999 |
NN |
denotes |
PCR |
T10387 |
991-992 |
-RRB- |
denotes |
) |
T10388 |
993-995 |
NN |
denotes |
RT |
T10389 |
995-996 |
HYPH |
denotes |
- |
T10390 |
1000-1002 |
IN |
denotes |
of |
T10391 |
1003-1009 |
NN |
denotes |
Trip13 |
T10392 |
1010-1013 |
CC |
denotes |
and |
T10393 |
1014-1015 |
DT |
denotes |
a |
T10394 |
1029-1034 |
NN |
denotes |
Med31 |
T10395 |
1016-1023 |
NN |
denotes |
control |
T10396 |
1024-1028 |
NN |
denotes |
gene |
T10397 |
1035-1039 |
IN |
denotes |
from |
T10398 |
1040-1046 |
NN |
denotes |
testis |
T10399 |
1047-1050 |
NN |
denotes |
RNA |
T10400 |
1050-1051 |
. |
denotes |
. |
T10401 |
1051-1140 |
sentence |
denotes |
The Trip13 primers are situated in the first and last exons (see Materials and Methods). |
T10402 |
1052-1055 |
DT |
denotes |
The |
T10403 |
1063-1070 |
NNS |
denotes |
primers |
T10404 |
1056-1062 |
NN |
denotes |
Trip13 |
T10405 |
1075-1083 |
VBN |
denotes |
situated |
T10406 |
1071-1074 |
VBP |
denotes |
are |
T10407 |
1084-1086 |
IN |
denotes |
in |
T10408 |
1087-1090 |
DT |
denotes |
the |
T10409 |
1106-1111 |
NNS |
denotes |
exons |
T10410 |
1091-1096 |
JJ |
denotes |
first |
T10411 |
1097-1100 |
CC |
denotes |
and |
T10412 |
1101-1105 |
JJ |
denotes |
last |
T10413 |
1112-1113 |
-LRB- |
denotes |
( |
T10414 |
1113-1116 |
VB |
denotes |
see |
T10415 |
1117-1126 |
NNS |
denotes |
Materials |
T10416 |
1127-1130 |
CC |
denotes |
and |
T10417 |
1131-1138 |
NNS |
denotes |
Methods |
T10418 |
1138-1139 |
-RRB- |
denotes |
) |
T10419 |
1139-1140 |
. |
denotes |
. |
T10420 |
1140-1211 |
sentence |
denotes |
(E) Western blot analysis of testis protein with anti-TRIP13 antibody. |
T10421 |
1141-1142 |
-LRB- |
denotes |
( |
T10422 |
1142-1143 |
LS |
denotes |
E |
T10423 |
1158-1166 |
NN |
denotes |
analysis |
T10424 |
1143-1144 |
-RRB- |
denotes |
) |
T10425 |
1145-1152 |
NNP |
denotes |
Western |
T10426 |
1153-1157 |
NN |
denotes |
blot |
T10427 |
1167-1169 |
IN |
denotes |
of |
T10428 |
1170-1176 |
NN |
denotes |
testis |
T10429 |
1177-1184 |
NN |
denotes |
protein |
T10430 |
1185-1189 |
IN |
denotes |
with |
T10431 |
1190-1201 |
JJ |
denotes |
anti-TRIP13 |
T10432 |
1202-1210 |
NN |
denotes |
antibody |
T10433 |
1210-1211 |
. |
denotes |
. |
T10434 |
1211-1289 |
sentence |
denotes |
The blot was later probed with anti-alpha tubulin actin as a loading control. |
T10435 |
1212-1215 |
DT |
denotes |
The |
T10436 |
1216-1220 |
NN |
denotes |
blot |
T10437 |
1231-1237 |
VBN |
denotes |
probed |
T10438 |
1221-1224 |
VBD |
denotes |
was |
T10439 |
1225-1230 |
RB |
denotes |
later |
T10440 |
1238-1242 |
IN |
denotes |
with |
T10441 |
1243-1253 |
JJ |
denotes |
anti-alpha |
T10442 |
1262-1267 |
NN |
denotes |
actin |
T10443 |
1254-1261 |
NN |
denotes |
tubulin |
T10444 |
1268-1270 |
IN |
denotes |
as |
T10445 |
1271-1272 |
DT |
denotes |
a |
T10446 |
1281-1288 |
NN |
denotes |
control |
T10447 |
1273-1280 |
NN |
denotes |
loading |
T10448 |
1288-1289 |
. |
denotes |
. |
T10449 |
1289-1329 |
sentence |
denotes |
The expected TRIP13 protein is ∼48 KDa. |
T10450 |
1290-1293 |
DT |
denotes |
The |
T10451 |
1310-1317 |
NN |
denotes |
protein |
T10452 |
1294-1302 |
JJ |
denotes |
expected |
T10453 |
1303-1309 |
NN |
denotes |
TRIP13 |
T10454 |
1318-1320 |
VBZ |
denotes |
is |
T10455 |
1321-1322 |
SYM |
denotes |
∼ |
T10456 |
1322-1324 |
CD |
denotes |
48 |
T10457 |
1325-1328 |
NN |
denotes |
KDa |
T10458 |
1328-1329 |
. |
denotes |
. |
T10459 |
1329-1367 |
sentence |
denotes |
(F) Localization of TRIP13 in testes. |
T10460 |
1330-1331 |
-LRB- |
denotes |
( |
T10461 |
1331-1332 |
LS |
denotes |
F |
T10462 |
1334-1346 |
NN |
denotes |
Localization |
T10463 |
1332-1333 |
-RRB- |
denotes |
) |
T10464 |
1347-1349 |
IN |
denotes |
of |
T10465 |
1350-1356 |
NN |
denotes |
TRIP13 |
T10466 |
1357-1359 |
IN |
denotes |
in |
T10467 |
1360-1366 |
NNS |
denotes |
testes |
T10468 |
1366-1367 |
. |
denotes |
. |
T10469 |
1367-1529 |
sentence |
denotes |
Wild-type (top) and mutant (bottom) testis sections were probed with chicken anti-TRIP13, and detected with HRP-conjugated anti-chicken IgG (brown/red staining). |
T10470 |
1368-1372 |
JJ |
denotes |
Wild |
T10471 |
1373-1377 |
NN |
denotes |
type |
T10472 |
1372-1373 |
HYPH |
denotes |
- |
T10473 |
1404-1410 |
NN |
denotes |
testis |
T10474 |
1378-1379 |
-LRB- |
denotes |
( |
T10475 |
1379-1382 |
JJ |
denotes |
top |
T10476 |
1382-1383 |
-RRB- |
denotes |
) |
T10477 |
1384-1387 |
CC |
denotes |
and |
T10478 |
1388-1394 |
NN |
denotes |
mutant |
T10479 |
1395-1396 |
-LRB- |
denotes |
( |
T10480 |
1396-1402 |
JJ |
denotes |
bottom |
T10481 |
1402-1403 |
-RRB- |
denotes |
) |
T10482 |
1411-1419 |
NNS |
denotes |
sections |
T10483 |
1425-1431 |
VBN |
denotes |
probed |
T10484 |
1420-1424 |
VBD |
denotes |
were |
T10485 |
1432-1436 |
IN |
denotes |
with |
T10486 |
1437-1444 |
NN |
denotes |
chicken |
T10487 |
1445-1456 |
JJ |
denotes |
anti-TRIP13 |
T10488 |
1456-1458 |
, |
denotes |
, |
T10489 |
1458-1461 |
CC |
denotes |
and |
T10490 |
1462-1470 |
VBN |
denotes |
detected |
T10491 |
1471-1475 |
IN |
denotes |
with |
T10492 |
1476-1479 |
NN |
denotes |
HRP |
T10493 |
1480-1490 |
VBN |
denotes |
conjugated |
T10494 |
1479-1480 |
HYPH |
denotes |
- |
T10495 |
1504-1507 |
NN |
denotes |
IgG |
T10496 |
1491-1503 |
JJ |
denotes |
anti-chicken |
T10497 |
1508-1509 |
-LRB- |
denotes |
( |
T10498 |
1519-1527 |
NN |
denotes |
staining |
T10499 |
1509-1514 |
JJ |
denotes |
brown |
T10500 |
1515-1518 |
JJ |
denotes |
red |
T10501 |
1514-1515 |
HYPH |
denotes |
/ |
T10502 |
1527-1528 |
-RRB- |
denotes |
) |
T10503 |
1528-1529 |
. |
denotes |
. |
T10504 |
1529-1722 |
sentence |
denotes |
Expression in WT was most prominent in the nuclei of Type B spermatogonia (Sg), leptotene spermatocytes (LS), and early pachytene spermatocytes (PS), but not late pachytene spermatocytes (LP). |
T10505 |
1530-1540 |
NN |
denotes |
Expression |
T10506 |
1547-1550 |
VBD |
denotes |
was |
T10507 |
1541-1543 |
IN |
denotes |
in |
T10508 |
1544-1546 |
NN |
denotes |
WT |
T10509 |
1551-1555 |
RBS |
denotes |
most |
T10510 |
1556-1565 |
JJ |
denotes |
prominent |
T10511 |
1566-1568 |
IN |
denotes |
in |
T10512 |
1569-1572 |
DT |
denotes |
the |
T10513 |
1573-1579 |
NNS |
denotes |
nuclei |
T10514 |
1580-1582 |
IN |
denotes |
of |
T10515 |
1583-1587 |
NN |
denotes |
Type |
T10516 |
1588-1589 |
NN |
denotes |
B |
T10517 |
1590-1603 |
NNS |
denotes |
spermatogonia |
T10518 |
1604-1605 |
-LRB- |
denotes |
( |
T10519 |
1605-1607 |
NN |
denotes |
Sg |
T10520 |
1607-1608 |
-RRB- |
denotes |
) |
T10521 |
1608-1610 |
, |
denotes |
, |
T10522 |
1610-1619 |
NN |
denotes |
leptotene |
T10523 |
1620-1633 |
NNS |
denotes |
spermatocytes |
T10524 |
1634-1635 |
-LRB- |
denotes |
( |
T10525 |
1635-1637 |
NNS |
denotes |
LS |
T10526 |
1637-1638 |
-RRB- |
denotes |
) |
T10527 |
1638-1640 |
, |
denotes |
, |
T10528 |
1640-1643 |
CC |
denotes |
and |
T10529 |
1644-1649 |
JJ |
denotes |
early |
T10530 |
1660-1673 |
NNS |
denotes |
spermatocytes |
T10531 |
1650-1659 |
NN |
denotes |
pachytene |
T10532 |
1674-1675 |
-LRB- |
denotes |
( |
T10533 |
1675-1677 |
NN |
denotes |
PS |
T10534 |
1677-1678 |
-RRB- |
denotes |
) |
T10535 |
1678-1680 |
, |
denotes |
, |
T10536 |
1680-1683 |
CC |
denotes |
but |
T10537 |
1684-1687 |
RB |
denotes |
not |
T10538 |
1688-1692 |
JJ |
denotes |
late |
T10539 |
1703-1716 |
NNS |
denotes |
spermatocytes |
T10540 |
1693-1702 |
NN |
denotes |
pachytene |
T10541 |
1717-1718 |
-LRB- |
denotes |
( |
T10542 |
1718-1720 |
NN |
denotes |
LP |
T10543 |
1720-1721 |
-RRB- |
denotes |
) |
T10544 |
1721-1722 |
. |
denotes |
. |
T10545 |
1722-1830 |
sentence |
denotes |
No nuclear staining was seen in mutant testis sections, although reddish cytoplasmic background is present. |
T10546 |
1723-1725 |
DT |
denotes |
No |
T10547 |
1734-1742 |
NN |
denotes |
staining |
T10548 |
1726-1733 |
JJ |
denotes |
nuclear |
T10549 |
1747-1751 |
VBN |
denotes |
seen |
T10550 |
1743-1746 |
VBD |
denotes |
was |
T10551 |
1752-1754 |
IN |
denotes |
in |
T10552 |
1755-1761 |
NN |
denotes |
mutant |
T10553 |
1769-1777 |
NNS |
denotes |
sections |
T10554 |
1762-1768 |
NN |
denotes |
testis |
T10555 |
1777-1779 |
, |
denotes |
, |
T10556 |
1779-1787 |
IN |
denotes |
although |
T10557 |
1819-1821 |
VBZ |
denotes |
is |
T10558 |
1788-1795 |
JJ |
denotes |
reddish |
T10559 |
1808-1818 |
NN |
denotes |
background |
T10560 |
1796-1807 |
JJ |
denotes |
cytoplasmic |
T10561 |
1822-1829 |
JJ |
denotes |
present |
T10562 |
1829-1830 |
. |
denotes |
. |
T10563 |
1830-1947 |
sentence |
denotes |
Identification of cell types was judged in part by estimating the epithelial stage of the tubules as described [67]. |
T10564 |
1831-1845 |
NN |
denotes |
Identification |
T10565 |
1864-1870 |
VBN |
denotes |
judged |
T10566 |
1846-1848 |
IN |
denotes |
of |
T10567 |
1849-1853 |
NN |
denotes |
cell |
T10568 |
1854-1859 |
NNS |
denotes |
types |
T10569 |
1860-1863 |
VBD |
denotes |
was |
T10570 |
1871-1873 |
IN |
denotes |
in |
T10571 |
1874-1878 |
JJ |
denotes |
part |
T10572 |
1879-1881 |
IN |
denotes |
by |
T10573 |
1882-1892 |
VBG |
denotes |
estimating |
T10574 |
1893-1896 |
DT |
denotes |
the |
T10575 |
1908-1913 |
NN |
denotes |
stage |
T10576 |
1897-1907 |
JJ |
denotes |
epithelial |
T10577 |
1914-1916 |
IN |
denotes |
of |
T10578 |
1917-1920 |
DT |
denotes |
the |
T10579 |
1921-1928 |
NNS |
denotes |
tubules |
T10580 |
1929-1931 |
IN |
denotes |
as |
T10581 |
1932-1941 |
VBN |
denotes |
described |
T10582 |
1942-1943 |
-LRB- |
denotes |
[ |
T10583 |
1943-1945 |
CD |
denotes |
67 |
T10584 |
1945-1946 |
-RRB- |
denotes |
] |
T10585 |
1946-1947 |
. |
denotes |
. |
T10586 |
1947-2004 |
sentence |
denotes |
(G) TRIP13 localization in surface-spread spermatocytes. |
T10587 |
1948-1949 |
-LRB- |
denotes |
( |
T10588 |
1949-1950 |
LS |
denotes |
G |
T10589 |
1959-1971 |
NN |
denotes |
localization |
T10590 |
1950-1951 |
-RRB- |
denotes |
) |
T10591 |
1952-1958 |
NN |
denotes |
TRIP13 |
T10592 |
1972-1974 |
IN |
denotes |
in |
T10593 |
1975-1982 |
NN |
denotes |
surface |
T10594 |
1983-1989 |
NN |
denotes |
spread |
T10595 |
1982-1983 |
HYPH |
denotes |
- |
T10596 |
1990-2003 |
NNS |
denotes |
spermatocytes |
T10597 |
2003-2004 |
. |
denotes |
. |
T10598 |
2004-2072 |
sentence |
denotes |
Preparations were immunolabeled with anti-SYCP3 (S) and TRIP13 (T). |
T10599 |
2005-2017 |
NNS |
denotes |
Preparations |
T10600 |
2023-2036 |
VBN |
denotes |
immunolabeled |
T10601 |
2018-2022 |
VBD |
denotes |
were |
T10602 |
2037-2041 |
IN |
denotes |
with |
T10603 |
2042-2052 |
JJ |
denotes |
anti-SYCP3 |
T10604 |
2053-2054 |
-LRB- |
denotes |
( |
T10605 |
2054-2055 |
NN |
denotes |
S |
T10606 |
2055-2056 |
-RRB- |
denotes |
) |
T10607 |
2057-2060 |
CC |
denotes |
and |
T10608 |
2061-2067 |
NN |
denotes |
TRIP13 |
T10609 |
2068-2069 |
-LRB- |
denotes |
( |
T10610 |
2069-2070 |
NN |
denotes |
T |
T10611 |
2070-2071 |
-RRB- |
denotes |
) |
T10612 |
2071-2072 |
. |
denotes |
. |
T10613 |
2072-2188 |
sentence |
denotes |
Both individual and merged images are shown for leptotene (Lep), zygotene (Zyg), and pachytene (Pac) spermatocytes. |
T10614 |
2073-2077 |
CC |
denotes |
Both |
T10615 |
2078-2088 |
JJ |
denotes |
individual |
T10616 |
2100-2106 |
NNS |
denotes |
images |
T10617 |
2089-2092 |
CC |
denotes |
and |
T10618 |
2093-2099 |
VBN |
denotes |
merged |
T10619 |
2111-2116 |
VBN |
denotes |
shown |
T10620 |
2107-2110 |
VBP |
denotes |
are |
T10621 |
2117-2120 |
IN |
denotes |
for |
T10622 |
2121-2130 |
NN |
denotes |
leptotene |
T10623 |
2174-2187 |
NNS |
denotes |
spermatocytes |
T10624 |
2131-2132 |
-LRB- |
denotes |
( |
T10625 |
2132-2135 |
NN |
denotes |
Lep |
T10626 |
2135-2136 |
-RRB- |
denotes |
) |
T10627 |
2136-2138 |
, |
denotes |
, |
T10628 |
2138-2146 |
NN |
denotes |
zygotene |
T10629 |
2147-2148 |
-LRB- |
denotes |
( |
T10630 |
2148-2151 |
NN |
denotes |
Zyg |
T10631 |
2151-2152 |
-RRB- |
denotes |
) |
T10632 |
2152-2154 |
, |
denotes |
, |
T10633 |
2154-2157 |
CC |
denotes |
and |
T10634 |
2158-2167 |
NN |
denotes |
pachytene |
T10635 |
2168-2169 |
-LRB- |
denotes |
( |
T10636 |
2169-2172 |
NN |
denotes |
Pac |
T10637 |
2172-2173 |
-RRB- |
denotes |
) |
T10638 |
2187-2188 |
. |
denotes |
. |
T10639 |
2188-2231 |
sentence |
denotes |
Nuclear staining was absent in the mutant. |
T10640 |
2189-2196 |
JJ |
denotes |
Nuclear |
T10641 |
2197-2205 |
NN |
denotes |
staining |
T10642 |
2206-2209 |
VBD |
denotes |
was |
T10643 |
2210-2216 |
JJ |
denotes |
absent |
T10644 |
2217-2219 |
IN |
denotes |
in |
T10645 |
2220-2223 |
DT |
denotes |
the |
T10646 |
2224-2230 |
NN |
denotes |
mutant |
T10647 |
2230-2231 |
. |
denotes |
. |
R6842 |
T10169 |
T10170 |
det |
The,Ortholog |
R6843 |
T10171 |
T10172 |
compound |
Mouse,PCH2 |
R6844 |
T10172 |
T10170 |
compound |
PCH2,Ortholog |
R6845 |
T10173 |
T10170 |
appos |
TRIP13,Ortholog |
R6846 |
T10174 |
T10170 |
cc |
and,Ortholog |
R6847 |
T10175 |
T10170 |
conj |
Expression,Ortholog |
R6848 |
T10176 |
T10175 |
prep |
in,Expression |
R6849 |
T10177 |
T10178 |
amod |
Wild,Type |
R6850 |
T10178 |
T10176 |
pobj |
Type,in |
R6851 |
T10179 |
T10178 |
cc |
and,Type |
R6852 |
T10180 |
T10178 |
conj |
Mutant,Type |
R6853 |
T10182 |
T10183 |
punct |
(,A |
R6854 |
T10183 |
T10184 |
meta |
A,tree |
R6855 |
T10185 |
T10183 |
punct |
),A |
R6856 |
T10186 |
T10184 |
amod |
Phylogenetic,tree |
R6857 |
T10187 |
T10184 |
prep |
of,tree |
R6858 |
T10188 |
T10189 |
amod |
presumed,orthologs |
R6859 |
T10189 |
T10187 |
pobj |
orthologs,of |
R6860 |
T10190 |
T10191 |
compound |
PCH2,TRIP13 |
R6861 |
T10191 |
T10189 |
compound |
TRIP13,orthologs |
R6862 |
T10192 |
T10191 |
punct |
/,TRIP13 |
R6863 |
T10193 |
T10184 |
punct |
.,tree |
R6864 |
T10195 |
T10196 |
det |
The,number |
R6865 |
T10196 |
T10200 |
nsubjpass |
number,presented |
R6866 |
T10197 |
T10198 |
compound |
database,sequence |
R6867 |
T10198 |
T10199 |
compound |
sequence,accession |
R6868 |
T10199 |
T10196 |
compound |
accession,number |
R6869 |
T10201 |
T10196 |
prep |
of,number |
R6870 |
T10202 |
T10203 |
det |
each,protein |
R6871 |
T10203 |
T10201 |
pobj |
protein,of |
R6872 |
T10204 |
T10200 |
auxpass |
is,presented |
R6873 |
T10205 |
T10200 |
prep |
in,presented |
R6874 |
T10206 |
T10207 |
compound |
Table,S1 |
R6875 |
T10207 |
T10205 |
pobj |
S1,in |
R6876 |
T10208 |
T10200 |
punct |
.,presented |
R6877 |
T10210 |
T10211 |
nsubj |
Numbers,are |
R6878 |
T10212 |
T10210 |
acl |
shown,Numbers |
R6879 |
T10213 |
T10214 |
compound |
bootstrap,values |
R6880 |
T10214 |
T10211 |
attr |
values,are |
R6881 |
T10215 |
T10216 |
punct |
(,see |
R6882 |
T10216 |
T10211 |
parataxis |
see,are |
R6883 |
T10217 |
T10216 |
dobj |
Materials,see |
R6884 |
T10218 |
T10217 |
cc |
and,Materials |
R6885 |
T10219 |
T10217 |
conj |
Methods,Materials |
R6886 |
T10220 |
T10216 |
punct |
),see |
R6887 |
T10221 |
T10211 |
punct |
.,are |
R6888 |
T10223 |
T10224 |
det |
The,clustering |
R6889 |
T10224 |
T10225 |
nsubj |
clustering,was |
R6890 |
T10226 |
T10227 |
det |
the,same |
R6891 |
T10227 |
T10225 |
attr |
same,was |
R6892 |
T10228 |
T10225 |
advmod |
regardless,was |
R6893 |
T10229 |
T10228 |
prep |
of,regardless |
R6894 |
T10230 |
T10231 |
mark |
whether,used |
R6895 |
T10231 |
T10229 |
pcomp |
used,of |
R6896 |
T10232 |
T10233 |
det |
the,sequence |
R6897 |
T10233 |
T10231 |
nsubjpass |
sequence,used |
R6898 |
T10234 |
T10235 |
amod |
whole,entire |
R6899 |
T10235 |
T10233 |
amod |
entire,sequence |
R6900 |
T10236 |
T10233 |
compound |
AA,sequence |
R6901 |
T10237 |
T10233 |
cc |
or,sequence |
R6902 |
T10238 |
T10239 |
amod |
trimmed,sequences |
R6903 |
T10239 |
T10233 |
conj |
sequences,sequence |
R6904 |
T10240 |
T10239 |
punct |
(,sequences |
R6905 |
T10241 |
T10242 |
advmod |
where,removed |
R6906 |
T10242 |
T10239 |
relcl |
removed,sequences |
R6907 |
T10243 |
T10242 |
nsubjpass |
regions,removed |
R6908 |
T10244 |
T10243 |
acl |
showing,regions |
R6909 |
T10245 |
T10246 |
amod |
little,conservation |
R6910 |
T10246 |
T10244 |
dobj |
conservation,showing |
R6911 |
T10247 |
T10242 |
auxpass |
were,removed |
R6912 |
T10248 |
T10231 |
punct |
),used |
R6913 |
T10249 |
T10231 |
auxpass |
were,used |
R6914 |
T10250 |
T10231 |
prep |
for,used |
R6915 |
T10251 |
T10252 |
det |
the,analysis |
R6916 |
T10252 |
T10250 |
pobj |
analysis,for |
R6917 |
T10253 |
T10225 |
punct |
.,was |
R6918 |
T10255 |
T10256 |
amod |
Major,groups |
R6919 |
T10256 |
T10258 |
nsubjpass |
groups,indicated |
R6920 |
T10257 |
T10256 |
amod |
eukaryotic,groups |
R6921 |
T10259 |
T10258 |
auxpass |
are,indicated |
R6922 |
T10260 |
T10258 |
prep |
in,indicated |
R6923 |
T10261 |
T10260 |
pobj |
color,in |
R6924 |
T10262 |
T10258 |
punct |
", ",indicated |
R6925 |
T10263 |
T10264 |
mark |
with,in |
R6926 |
T10264 |
T10258 |
advcl |
in,indicated |
R6927 |
T10265 |
T10264 |
nsubj |
deuterostomia,in |
R6928 |
T10266 |
T10264 |
pobj |
blue,in |
R6929 |
T10267 |
T10264 |
punct |
", ",in |
R6930 |
T10268 |
T10269 |
nsubj |
plants,in |
R6931 |
T10269 |
T10264 |
conj |
in,in |
R6932 |
T10270 |
T10269 |
pobj |
green,in |
R6933 |
T10271 |
T10269 |
punct |
", ",in |
R6934 |
T10272 |
T10273 |
nsubj |
protostomia,in |
R6935 |
T10273 |
T10269 |
conj |
in,in |
R6936 |
T10274 |
T10273 |
pobj |
purple,in |
R6937 |
T10275 |
T10273 |
punct |
", ",in |
R6938 |
T10276 |
T10273 |
cc |
and,in |
R6939 |
T10277 |
T10278 |
nsubj |
fungi,in |
R6940 |
T10278 |
T10273 |
conj |
in,in |
R6941 |
T10279 |
T10278 |
pobj |
maroon,in |
R6942 |
T10280 |
T10258 |
punct |
.,indicated |
R6943 |
T10282 |
T10283 |
punct |
(,B |
R6944 |
T10283 |
T10284 |
meta |
B,products |
R6945 |
T10285 |
T10283 |
punct |
),B |
R6946 |
T10286 |
T10284 |
compound |
Amplification,products |
R6947 |
T10287 |
T10284 |
prep |
of,products |
R6948 |
T10288 |
T10287 |
pobj |
cDNA,of |
R6949 |
T10289 |
T10284 |
prep |
from,products |
R6950 |
T10290 |
T10291 |
det |
the,tissues |
R6951 |
T10291 |
T10289 |
pobj |
tissues,from |
R6952 |
T10292 |
T10291 |
amod |
following,tissues |
R6953 |
T10293 |
T10291 |
punct |
: ,tissues |
R6954 |
T10294 |
T10295 |
meta |
1,heart |
R6955 |
T10295 |
T10291 |
appos |
heart,tissues |
R6956 |
T10296 |
T10295 |
punct |
", ",heart |
R6957 |
T10297 |
T10295 |
punct |
;,heart |
R6958 |
T10298 |
T10299 |
meta |
2,brain |
R6959 |
T10299 |
T10295 |
conj |
brain,heart |
R6960 |
T10300 |
T10299 |
punct |
", ",brain |
R6961 |
T10301 |
T10299 |
punct |
;,brain |
R6962 |
T10302 |
T10303 |
meta |
3,spleen |
R6963 |
T10303 |
T10299 |
conj |
spleen,brain |
R6964 |
T10304 |
T10303 |
punct |
", ",spleen |
R6965 |
T10305 |
T10303 |
punct |
;,spleen |
R6966 |
T10306 |
T10307 |
meta |
4,lung |
R6967 |
T10307 |
T10303 |
conj |
lung,spleen |
R6968 |
T10308 |
T10307 |
punct |
", ",lung |
R6969 |
T10309 |
T10307 |
punct |
;,lung |
R6970 |
T10310 |
T10311 |
meta |
5,liver |
R6971 |
T10311 |
T10307 |
conj |
liver,lung |
R6972 |
T10312 |
T10311 |
punct |
", ",liver |
R6973 |
T10313 |
T10311 |
punct |
;,liver |
R6974 |
T10314 |
T10315 |
meta |
6,muscle |
R6975 |
T10315 |
T10311 |
conj |
muscle,liver |
R6976 |
T10316 |
T10315 |
punct |
", ",muscle |
R6977 |
T10317 |
T10315 |
amod |
skeletal,muscle |
R6978 |
T10318 |
T10315 |
punct |
;,muscle |
R6979 |
T10319 |
T10320 |
meta |
7,kidney |
R6980 |
T10320 |
T10315 |
conj |
kidney,muscle |
R6981 |
T10321 |
T10320 |
punct |
", ",kidney |
R6982 |
T10322 |
T10320 |
punct |
;,kidney |
R6983 |
T10323 |
T10324 |
meta |
8,testis |
R6984 |
T10324 |
T10320 |
conj |
testis,kidney |
R6985 |
T10325 |
T10324 |
punct |
", ",testis |
R6986 |
T10326 |
T10324 |
punct |
;,testis |
R6987 |
T10327 |
T10328 |
meta |
9,embryo |
R6988 |
T10328 |
T10324 |
conj |
embryo,testis |
R6989 |
T10329 |
T10328 |
punct |
", ",embryo |
R6990 |
T10330 |
T10328 |
compound |
E7,embryo |
R6991 |
T10331 |
T10328 |
punct |
;,embryo |
R6992 |
T10332 |
T10333 |
meta |
10,embryo |
R6993 |
T10333 |
T10328 |
conj |
embryo,embryo |
R6994 |
T10334 |
T10333 |
punct |
", ",embryo |
R6995 |
T10335 |
T10333 |
compound |
E11,embryo |
R6996 |
T10336 |
T10333 |
punct |
;,embryo |
R6997 |
T10337 |
T10338 |
meta |
11,embryo |
R6998 |
T10338 |
T10333 |
conj |
embryo,embryo |
R6999 |
T10339 |
T10338 |
punct |
", ",embryo |
R7000 |
T10340 |
T10338 |
compound |
E15,embryo |
R7001 |
T10341 |
T10338 |
punct |
;,embryo |
R7002 |
T10342 |
T10338 |
cc |
and,embryo |
R7003 |
T10343 |
T10344 |
meta |
12,embryo |
R7004 |
T10344 |
T10338 |
conj |
embryo,embryo |
R7005 |
T10345 |
T10344 |
punct |
", ",embryo |
R7006 |
T10346 |
T10344 |
compound |
E17,embryo |
R7007 |
T10347 |
T10284 |
punct |
.,products |
R7008 |
T10349 |
T10350 |
punct |
(,C |
R7009 |
T10350 |
T10351 |
meta |
C,structure |
R7010 |
T10352 |
T10350 |
punct |
),C |
R7011 |
T10353 |
T10354 |
compound |
Intron,exon |
R7012 |
T10354 |
T10351 |
compound |
exon,structure |
R7013 |
T10355 |
T10354 |
punct |
–,exon |
R7014 |
T10356 |
T10351 |
prep |
of,structure |
R7015 |
T10357 |
T10356 |
pobj |
TRIP13,of |
R7016 |
T10358 |
T10351 |
cc |
and,structure |
R7017 |
T10359 |
T10360 |
compound |
insertion,site |
R7018 |
T10360 |
T10351 |
conj |
site,structure |
R7019 |
T10361 |
T10360 |
prep |
of,site |
R7020 |
T10362 |
T10363 |
compound |
gene,trap |
R7021 |
T10363 |
T10365 |
compound |
trap,vector |
R7022 |
T10364 |
T10363 |
punct |
-,trap |
R7023 |
T10365 |
T10361 |
pobj |
vector,of |
R7024 |
T10366 |
T10360 |
punct |
.,site |
R7025 |
T10369 |
T10368 |
dobj |
Materials,See |
R7026 |
T10370 |
T10369 |
cc |
and,Materials |
R7027 |
T10371 |
T10369 |
conj |
Methods,Materials |
R7028 |
T10372 |
T10368 |
prep |
for,See |
R7029 |
T10373 |
T10372 |
pobj |
details,for |
R7030 |
T10374 |
T10373 |
prep |
on,details |
R7031 |
T10375 |
T10376 |
advmod |
how,identified |
R7032 |
T10376 |
T10374 |
pcomp |
identified,on |
R7033 |
T10377 |
T10378 |
det |
the,site |
R7034 |
T10378 |
T10376 |
nsubjpass |
site,identified |
R7035 |
T10379 |
T10378 |
amod |
precise,site |
R7036 |
T10380 |
T10378 |
compound |
insertion,site |
R7037 |
T10381 |
T10376 |
auxpass |
was,identified |
R7038 |
T10382 |
T10368 |
punct |
.,See |
R7039 |
T10384 |
T10385 |
punct |
(,D |
R7040 |
T10385 |
T10386 |
meta |
D,PCR |
R7041 |
T10387 |
T10385 |
punct |
),D |
R7042 |
T10388 |
T10386 |
compound |
RT,PCR |
R7043 |
T10389 |
T10386 |
punct |
-,PCR |
R7044 |
T10390 |
T10386 |
prep |
of,PCR |
R7045 |
T10391 |
T10390 |
pobj |
Trip13,of |
R7046 |
T10392 |
T10391 |
cc |
and,Trip13 |
R7047 |
T10393 |
T10394 |
det |
a,Med31 |
R7048 |
T10394 |
T10391 |
conj |
Med31,Trip13 |
R7049 |
T10395 |
T10394 |
compound |
control,Med31 |
R7050 |
T10396 |
T10394 |
compound |
gene,Med31 |
R7051 |
T10397 |
T10394 |
prep |
from,Med31 |
R7052 |
T10398 |
T10399 |
compound |
testis,RNA |
R7053 |
T10399 |
T10397 |
pobj |
RNA,from |
R7054 |
T10400 |
T10386 |
punct |
.,PCR |
R7055 |
T10402 |
T10403 |
det |
The,primers |
R7056 |
T10403 |
T10405 |
nsubjpass |
primers,situated |
R7057 |
T10404 |
T10403 |
compound |
Trip13,primers |
R7058 |
T10406 |
T10405 |
auxpass |
are,situated |
R7059 |
T10407 |
T10405 |
prep |
in,situated |
R7060 |
T10408 |
T10409 |
det |
the,exons |
R7061 |
T10409 |
T10407 |
pobj |
exons,in |
R7062 |
T10410 |
T10409 |
amod |
first,exons |
R7063 |
T10411 |
T10410 |
cc |
and,first |
R7064 |
T10412 |
T10410 |
conj |
last,first |
R7065 |
T10413 |
T10414 |
punct |
(,see |
R7066 |
T10414 |
T10405 |
parataxis |
see,situated |
R7067 |
T10415 |
T10414 |
dobj |
Materials,see |
R7068 |
T10416 |
T10415 |
cc |
and,Materials |
R7069 |
T10417 |
T10415 |
conj |
Methods,Materials |
R7070 |
T10418 |
T10414 |
punct |
),see |
R7071 |
T10419 |
T10405 |
punct |
.,situated |
R7072 |
T10421 |
T10422 |
punct |
(,E |
R7073 |
T10422 |
T10423 |
meta |
E,analysis |
R7074 |
T10424 |
T10422 |
punct |
),E |
R7075 |
T10425 |
T10426 |
compound |
Western,blot |
R7076 |
T10426 |
T10423 |
compound |
blot,analysis |
R7077 |
T10427 |
T10423 |
prep |
of,analysis |
R7078 |
T10428 |
T10429 |
compound |
testis,protein |
R7079 |
T10429 |
T10427 |
pobj |
protein,of |
R7080 |
T10430 |
T10423 |
prep |
with,analysis |
R7081 |
T10431 |
T10432 |
amod |
anti-TRIP13,antibody |
R7082 |
T10432 |
T10430 |
pobj |
antibody,with |
R7083 |
T10433 |
T10423 |
punct |
.,analysis |
R7084 |
T10435 |
T10436 |
det |
The,blot |
R7085 |
T10436 |
T10437 |
nsubjpass |
blot,probed |
R7086 |
T10438 |
T10437 |
auxpass |
was,probed |
R7087 |
T10439 |
T10437 |
advmod |
later,probed |
R7088 |
T10440 |
T10437 |
prep |
with,probed |
R7089 |
T10441 |
T10442 |
amod |
anti-alpha,actin |
R7090 |
T10442 |
T10440 |
pobj |
actin,with |
R7091 |
T10443 |
T10442 |
compound |
tubulin,actin |
R7092 |
T10444 |
T10442 |
prep |
as,actin |
R7093 |
T10445 |
T10446 |
det |
a,control |
R7094 |
T10446 |
T10444 |
pobj |
control,as |
R7095 |
T10447 |
T10446 |
compound |
loading,control |
R7096 |
T10448 |
T10437 |
punct |
.,probed |
R7097 |
T10450 |
T10451 |
det |
The,protein |
R7098 |
T10451 |
T10454 |
nsubj |
protein,is |
R7099 |
T10452 |
T10451 |
amod |
expected,protein |
R7100 |
T10453 |
T10451 |
compound |
TRIP13,protein |
R7101 |
T10455 |
T10456 |
punct |
∼,48 |
R7102 |
T10456 |
T10457 |
nummod |
48,KDa |
R7103 |
T10457 |
T10454 |
attr |
KDa,is |
R7104 |
T10458 |
T10454 |
punct |
.,is |
R7105 |
T10460 |
T10461 |
punct |
(,F |
R7106 |
T10461 |
T10462 |
meta |
F,Localization |
R7107 |
T10463 |
T10461 |
punct |
),F |
R7108 |
T10464 |
T10462 |
prep |
of,Localization |
R7109 |
T10465 |
T10464 |
pobj |
TRIP13,of |
R7110 |
T10466 |
T10462 |
prep |
in,Localization |
R7111 |
T10467 |
T10466 |
pobj |
testes,in |
R7112 |
T10468 |
T10462 |
punct |
.,Localization |
R7113 |
T10470 |
T10471 |
amod |
Wild,type |
R7114 |
T10471 |
T10473 |
nmod |
type,testis |
R7115 |
T10472 |
T10471 |
punct |
-,type |
R7116 |
T10473 |
T10482 |
compound |
testis,sections |
R7117 |
T10474 |
T10475 |
punct |
(,top |
R7118 |
T10475 |
T10471 |
parataxis |
top,type |
R7119 |
T10476 |
T10475 |
punct |
),top |
R7120 |
T10477 |
T10471 |
cc |
and,type |
R7121 |
T10478 |
T10471 |
conj |
mutant,type |
R7122 |
T10479 |
T10480 |
punct |
(,bottom |
R7123 |
T10480 |
T10478 |
parataxis |
bottom,mutant |
R7124 |
T10481 |
T10480 |
punct |
),bottom |
R7125 |
T10482 |
T10483 |
nsubjpass |
sections,probed |
R7126 |
T10484 |
T10483 |
auxpass |
were,probed |
R7127 |
T10485 |
T10483 |
prep |
with,probed |
R7128 |
T10486 |
T10485 |
pobj |
chicken,with |
R7129 |
T10487 |
T10486 |
amod |
anti-TRIP13,chicken |
R7130 |
T10488 |
T10483 |
punct |
", ",probed |
R7131 |
T10489 |
T10483 |
cc |
and,probed |
R7132 |
T10490 |
T10483 |
conj |
detected,probed |
R7133 |
T10491 |
T10490 |
prep |
with,detected |
R7134 |
T10492 |
T10493 |
npadvmod |
HRP,conjugated |
R7135 |
T10493 |
T10495 |
amod |
conjugated,IgG |
R7136 |
T10494 |
T10493 |
punct |
-,conjugated |
R7137 |
T10495 |
T10491 |
pobj |
IgG,with |
R7138 |
T10496 |
T10495 |
amod |
anti-chicken,IgG |
R7139 |
T10497 |
T10498 |
punct |
(,staining |
R7140 |
T10498 |
T10495 |
parataxis |
staining,IgG |
R7141 |
T10499 |
T10500 |
amod |
brown,red |
R7142 |
T10500 |
T10498 |
amod |
red,staining |
R7143 |
T10501 |
T10500 |
punct |
/,red |
R7144 |
T10502 |
T10498 |
punct |
),staining |
R7145 |
T10503 |
T10483 |
punct |
.,probed |
R7146 |
T10505 |
T10506 |
nsubj |
Expression,was |
R7147 |
T10507 |
T10505 |
prep |
in,Expression |
R7148 |
T10508 |
T10507 |
pobj |
WT,in |
R7149 |
T10509 |
T10510 |
advmod |
most,prominent |
R7150 |
T10510 |
T10506 |
acomp |
prominent,was |
R7151 |
T10511 |
T10506 |
prep |
in,was |
R7152 |
T10512 |
T10513 |
det |
the,nuclei |
R7153 |
T10513 |
T10511 |
pobj |
nuclei,in |
R7154 |
T10514 |
T10513 |
prep |
of,nuclei |
R7155 |
T10515 |
T10516 |
compound |
Type,B |
R7156 |
T10516 |
T10517 |
compound |
B,spermatogonia |
R7157 |
T10517 |
T10514 |
pobj |
spermatogonia,of |
R7158 |
T10518 |
T10517 |
punct |
(,spermatogonia |
R7159 |
T10519 |
T10517 |
appos |
Sg,spermatogonia |
R7160 |
T10520 |
T10517 |
punct |
),spermatogonia |
R7161 |
T10521 |
T10517 |
punct |
", ",spermatogonia |
R7162 |
T10522 |
T10523 |
compound |
leptotene,spermatocytes |
R7163 |
T10523 |
T10517 |
conj |
spermatocytes,spermatogonia |
R7164 |
T10524 |
T10523 |
punct |
(,spermatocytes |
R7165 |
T10525 |
T10523 |
appos |
LS,spermatocytes |
R7166 |
T10526 |
T10523 |
punct |
),spermatocytes |
R7167 |
T10527 |
T10523 |
punct |
", ",spermatocytes |
R7168 |
T10528 |
T10523 |
cc |
and,spermatocytes |
R7169 |
T10529 |
T10530 |
amod |
early,spermatocytes |
R7170 |
T10530 |
T10523 |
conj |
spermatocytes,spermatocytes |
R7171 |
T10531 |
T10530 |
compound |
pachytene,spermatocytes |
R7172 |
T10532 |
T10530 |
punct |
(,spermatocytes |
R7173 |
T10533 |
T10530 |
appos |
PS,spermatocytes |
R7174 |
T10534 |
T10530 |
punct |
),spermatocytes |
R7175 |
T10535 |
T10530 |
punct |
", ",spermatocytes |
R7176 |
T10536 |
T10530 |
cc |
but,spermatocytes |
R7177 |
T10537 |
T10536 |
neg |
not,but |
R7178 |
T10538 |
T10539 |
amod |
late,spermatocytes |
R7179 |
T10539 |
T10530 |
conj |
spermatocytes,spermatocytes |
R7180 |
T10540 |
T10539 |
compound |
pachytene,spermatocytes |
R7181 |
T10541 |
T10539 |
punct |
(,spermatocytes |
R7182 |
T10542 |
T10539 |
appos |
LP,spermatocytes |
R7183 |
T10543 |
T10506 |
punct |
),was |
R7184 |
T10544 |
T10506 |
punct |
.,was |
R7185 |
T10546 |
T10547 |
det |
No,staining |
R7186 |
T10547 |
T10549 |
nsubjpass |
staining,seen |
R7187 |
T10548 |
T10547 |
amod |
nuclear,staining |
R7188 |
T10550 |
T10549 |
auxpass |
was,seen |
R7189 |
T10551 |
T10549 |
prep |
in,seen |
R7190 |
T10552 |
T10553 |
compound |
mutant,sections |
R7191 |
T10553 |
T10551 |
pobj |
sections,in |
R7192 |
T10554 |
T10553 |
compound |
testis,sections |
R7193 |
T10555 |
T10549 |
punct |
", ",seen |
R7194 |
T10556 |
T10557 |
mark |
although,is |
R7195 |
T10557 |
T10549 |
advcl |
is,seen |
R7196 |
T10558 |
T10559 |
amod |
reddish,background |
R7197 |
T10559 |
T10557 |
nsubj |
background,is |
R7198 |
T10560 |
T10559 |
amod |
cytoplasmic,background |
R7199 |
T10561 |
T10557 |
acomp |
present,is |
R7200 |
T10562 |
T10549 |
punct |
.,seen |
R7201 |
T10564 |
T10565 |
nsubjpass |
Identification,judged |
R7202 |
T10566 |
T10564 |
prep |
of,Identification |
R7203 |
T10567 |
T10568 |
compound |
cell,types |
R7204 |
T10568 |
T10566 |
pobj |
types,of |
R7205 |
T10569 |
T10565 |
auxpass |
was,judged |
R7206 |
T10570 |
T10565 |
prep |
in,judged |
R7207 |
T10571 |
T10570 |
amod |
part,in |
R7208 |
T10572 |
T10565 |
prep |
by,judged |
R7209 |
T10573 |
T10572 |
pcomp |
estimating,by |
R7210 |
T10574 |
T10575 |
det |
the,stage |
R7211 |
T10575 |
T10573 |
dobj |
stage,estimating |
R7212 |
T10576 |
T10575 |
amod |
epithelial,stage |
R7213 |
T10577 |
T10575 |
prep |
of,stage |
R7214 |
T10578 |
T10579 |
det |
the,tubules |
R7215 |
T10579 |
T10577 |
pobj |
tubules,of |
R7216 |
T10580 |
T10581 |
mark |
as,described |
R7217 |
T10581 |
T10573 |
advcl |
described,estimating |
R7218 |
T10582 |
T10583 |
punct |
[,67 |
R7219 |
T10583 |
T10573 |
parataxis |
67,estimating |
R7220 |
T10584 |
T10583 |
punct |
],67 |
R7221 |
T10585 |
T10565 |
punct |
.,judged |
R7222 |
T10587 |
T10588 |
punct |
(,G |
R7223 |
T10588 |
T10589 |
meta |
G,localization |
R7224 |
T10590 |
T10588 |
punct |
),G |
R7225 |
T10591 |
T10589 |
compound |
TRIP13,localization |
R7226 |
T10592 |
T10589 |
prep |
in,localization |
R7227 |
T10593 |
T10594 |
compound |
surface,spread |
R7228 |
T10594 |
T10596 |
compound |
spread,spermatocytes |
R7229 |
T10595 |
T10594 |
punct |
-,spread |
R7230 |
T10596 |
T10592 |
pobj |
spermatocytes,in |
R7231 |
T10597 |
T10589 |
punct |
.,localization |
R7232 |
T10599 |
T10600 |
nsubjpass |
Preparations,immunolabeled |
R7233 |
T10601 |
T10600 |
auxpass |
were,immunolabeled |
R7234 |
T10602 |
T10600 |
prep |
with,immunolabeled |
R7235 |
T10603 |
T10602 |
pobj |
anti-SYCP3,with |
R7236 |
T10604 |
T10603 |
punct |
(,anti-SYCP3 |
R7237 |
T10605 |
T10603 |
appos |
S,anti-SYCP3 |
R7238 |
T10606 |
T10603 |
punct |
),anti-SYCP3 |
R7239 |
T10607 |
T10603 |
cc |
and,anti-SYCP3 |
R7240 |
T10608 |
T10603 |
conj |
TRIP13,anti-SYCP3 |
R7241 |
T10609 |
T10608 |
punct |
(,TRIP13 |
R7242 |
T10610 |
T10608 |
appos |
T,TRIP13 |
R7243 |
T10611 |
T10600 |
punct |
),immunolabeled |
R7244 |
T10612 |
T10600 |
punct |
.,immunolabeled |
R7245 |
T10614 |
T10615 |
preconj |
Both,individual |
R7246 |
T10615 |
T10616 |
amod |
individual,images |
R7247 |
T10616 |
T10619 |
nsubjpass |
images,shown |
R7248 |
T10617 |
T10615 |
cc |
and,individual |
R7249 |
T10618 |
T10615 |
conj |
merged,individual |
R7250 |
T10620 |
T10619 |
auxpass |
are,shown |
R7251 |
T10621 |
T10619 |
prep |
for,shown |
R7252 |
T10622 |
T10623 |
nmod |
leptotene,spermatocytes |
R7253 |
T10623 |
T10621 |
pobj |
spermatocytes,for |
R7254 |
T10624 |
T10622 |
punct |
(,leptotene |
R7255 |
T10625 |
T10622 |
appos |
Lep,leptotene |
R7256 |
T10626 |
T10622 |
punct |
),leptotene |
R7257 |
T10627 |
T10622 |
punct |
", ",leptotene |
R7258 |
T10628 |
T10622 |
conj |
zygotene,leptotene |
R7259 |
T10629 |
T10628 |
punct |
(,zygotene |
R7260 |
T10630 |
T10628 |
appos |
Zyg,zygotene |
R7261 |
T10631 |
T10628 |
punct |
),zygotene |
R7262 |
T10632 |
T10628 |
punct |
", ",zygotene |
R7263 |
T10633 |
T10628 |
cc |
and,zygotene |
R7264 |
T10634 |
T10628 |
conj |
pachytene,zygotene |
R7265 |
T10635 |
T10634 |
punct |
(,pachytene |
R7266 |
T10636 |
T10634 |
appos |
Pac,pachytene |
R7267 |
T10637 |
T10623 |
punct |
),spermatocytes |
R7268 |
T10638 |
T10619 |
punct |
.,shown |
R7269 |
T10640 |
T10641 |
amod |
Nuclear,staining |
R7270 |
T10641 |
T10642 |
nsubj |
staining,was |
R7271 |
T10643 |
T10642 |
acomp |
absent,was |
R7272 |
T10644 |
T10642 |
prep |
in,was |
R7273 |
T10645 |
T10646 |
det |
the,mutant |
R7274 |
T10646 |
T10644 |
pobj |
mutant,in |
R7275 |
T10647 |
T10642 |
punct |
.,was |