PMC:1941754 / 6650-7418 JSONTXT

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    craft-sa-dev

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Interestingly, phylogenetic analysis of TRIP13/Pch2p shows that the mammalian protein clusters more closely to plants than it does to the evolutionarily more closely related worms and flies (Figure 1A; see Discussion). Semi-quantitative reverse-transcriptase PCR (RT-PCR) analysis showed Trip13 mRNA to be expressed in a variety of embryonic and adult tissues, including testis (Figure 1B), consistent with mouse and human EST data summarized in Unigene (http://www.ncbi.nlm.nih.gov/UniGene). It is also highly expressed in human and mouse oocytes [17]."}

    craft-ca-core-dev

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    2_test

    {"project":"2_test","denotations":[{"id":"17696610-17174924-85497894","span":{"begin":209,"end":211},"obj":"17174924"},{"id":"17696610-16968779-85497895","span":{"begin":764,"end":766},"obj":"16968779"},{"id":"T19826","span":{"begin":209,"end":211},"obj":"17174924"},{"id":"T61584","span":{"begin":764,"end":766},"obj":"16968779"}],"text":"The mammalian ortholog of PCH2, Trip13 (thyroid hormone receptor interacting protein 13), encodes a protein with extensive amino acid homology in regions alignable to the yeast and worm orthologs (Figure S1) [12]). Interestingly, phylogenetic analysis of TRIP13/Pch2p shows that the mammalian protein clusters more closely to plants than it does to the evolutionarily more closely related worms and flies (Figure 1A; see Discussion). Semi-quantitative reverse-transcriptase PCR (RT-PCR) analysis showed Trip13 mRNA to be expressed in a variety of embryonic and adult tissues, including testis (Figure 1B), consistent with mouse and human EST data summarized in Unigene (http://www.ncbi.nlm.nih.gov/UniGene). It is also highly expressed in human and mouse oocytes [17]."}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T1367","span":{"begin":4,"end":13},"obj":"NCBITaxon:40674"},{"id":"T1368","span":{"begin":14,"end":22},"obj":"SO_EXT:0000855"},{"id":"T1369","span":{"begin":26,"end":30},"obj":"PR_EXT:P38126"},{"id":"T1370","span":{"begin":32,"end":38},"obj":"PR_EXT:000016672"},{"id":"T1371","span":{"begin":40,"end":47},"obj":"UBERON:0002046"},{"id":"T1372","span":{"begin":40,"end":55},"obj":"CHEBI_EXT:60311"},{"id":"T1373","span":{"begin":40,"end":64},"obj":"GO_EXT:0004887"},{"id":"T1374","span":{"begin":40,"end":87},"obj":"PR_EXT:000016672"},{"id":"T1375","span":{"begin":77,"end":84},"obj":"CHEBI_PR_EXT:protein"},{"id":"T1376","span":{"begin":90,"end":97},"obj":"SO_EXT:sequence_coding_function"},{"id":"T1377","span":{"begin":100,"end":107},"obj":"CHEBI_PR_EXT:protein"},{"id":"T1378","span":{"begin":123,"end":133},"obj":"CHEBI_SO_EXT:amino_acid"},{"id":"T1379","span":{"begin":134,"end":142},"obj":"SO:0000857"},{"id":"T1380","span":{"begin":171,"end":176},"obj":"NCBITaxon_EXT:yeast"},{"id":"T1381","span":{"begin":181,"end":185},"obj":"NCBITaxon_EXT:worm"},{"id":"T1382","span":{"begin":186,"end":195},"obj":"SO_EXT:0000855"},{"id":"T1383","span":{"begin":255,"end":261},"obj":"PR_EXT:000016672"},{"id":"T1384","span":{"begin":283,"end":292},"obj":"NCBITaxon:40674"},{"id":"T1385","span":{"begin":293,"end":300},"obj":"CHEBI_PR_EXT:protein"},{"id":"T1386","span":{"begin":326,"end":332},"obj":"NCBITaxon:3193"},{"id":"T1387","span":{"begin":353,"end":367},"obj":"_FRAGMENT"},{"id":"T1388","span":{"begin":381,"end":388},"obj":"SO:0000857"},{"id":"T1389","span":{"begin":389,"end":394},"obj":"NCBITaxon_EXT:worm"},{"id":"T1390","span":{"begin":399,"end":404},"obj":"NCBITaxon:7147"},{"id":"T1391","span":{"begin":452,"end":473},"obj":"GO_EXT:0003964"},{"id":"T1392","span":{"begin":479,"end":481},"obj":"GO_EXT:reverse_transcription_or_reverse_transcriptase"},{"id":"T1393","span":{"begin":503,"end":509},"obj":"PR_EXT:000016672"},{"id":"T1394","span":{"begin":510,"end":514},"obj":"CHEBI_SO_EXT:mRNA"},{"id":"T1395","span":{"begin":521,"end":530},"obj":"GO:0010467"},{"id":"T1396","span":{"begin":547,"end":556},"obj":"_FRAGMENT"},{"id":"T1397","span":{"begin":567,"end":574},"obj":"UBERON:0005291"},{"id":"T1398","span":{"begin":561,"end":566},"obj":"UBERON:0007023"},{"id":"T1399","span":{"begin":586,"end":592},"obj":"UBERON:0000473"},{"id":"T1400","span":{"begin":622,"end":627},"obj":"NCBITaxon:10088"},{"id":"T1401","span":{"begin":632,"end":637},"obj":"NCBITaxon:9606"},{"id":"T1402","span":{"begin":638,"end":641},"obj":"SO_EXT:0000345"},{"id":"T1403","span":{"begin":726,"end":735},"obj":"GO:0010467"},{"id":"T1404","span":{"begin":739,"end":744},"obj":"NCBITaxon:9606"},{"id":"T1405","span":{"begin":749,"end":754},"obj":"NCBITaxon:10088"},{"id":"T1406","span":{"begin":755,"end":762},"obj":"CL:0000023"}],"relations":[{"id":"R877","pred":"_lexicallyChainedTo","subj":"T1388","obj":"T1387"},{"id":"R878","pred":"_lexicallyChainedTo","subj":"T1397","obj":"T1396"}],"text":"The mammalian ortholog of PCH2, Trip13 (thyroid hormone receptor interacting protein 13), encodes a protein with extensive amino acid homology in regions alignable to the yeast and worm orthologs (Figure S1) [12]). Interestingly, phylogenetic analysis of TRIP13/Pch2p shows that the mammalian protein clusters more closely to plants than it does to the evolutionarily more closely related worms and flies (Figure 1A; see Discussion). Semi-quantitative reverse-transcriptase PCR (RT-PCR) analysis showed Trip13 mRNA to be expressed in a variety of embryonic and adult tissues, including testis (Figure 1B), consistent with mouse and human EST data summarized in Unigene (http://www.ncbi.nlm.nih.gov/UniGene). It is also highly expressed in human and mouse oocytes [17]."}