Id |
Subject |
Object |
Predicate |
Lexical cue |
T1407 |
0-6 |
NN |
denotes |
Trip13 |
T1408 |
7-9 |
VBZ |
denotes |
Is |
T1409 |
10-11 |
DT |
denotes |
a |
T1410 |
39-47 |
NN |
denotes |
Ortholog |
T1411 |
12-18 |
RB |
denotes |
Widely |
T1412 |
19-28 |
VBN |
denotes |
Expressed |
T1413 |
29-38 |
JJ |
denotes |
Mammalian |
T1414 |
48-50 |
IN |
denotes |
of |
T1415 |
51-55 |
NN |
denotes |
PCH2 |
T1416 |
56-60 |
IN |
denotes |
with |
T1417 |
61-68 |
JJ |
denotes |
Unusual |
T1418 |
82-95 |
NNS |
denotes |
Relationships |
T1419 |
69-81 |
JJ |
denotes |
Phylogenetic |
T1420 |
95-310 |
sentence |
denotes |
The mammalian ortholog of PCH2, Trip13 (thyroid hormone receptor interacting protein 13), encodes a protein with extensive amino acid homology in regions alignable to the yeast and worm orthologs (Figure S1) [12]). |
T1421 |
96-99 |
DT |
denotes |
The |
T1422 |
110-118 |
NN |
denotes |
ortholog |
T1423 |
100-109 |
JJ |
denotes |
mammalian |
T1424 |
186-193 |
VBZ |
denotes |
encodes |
T1425 |
119-121 |
IN |
denotes |
of |
T1426 |
122-126 |
NN |
denotes |
PCH2 |
T1427 |
126-128 |
, |
denotes |
, |
T1428 |
128-134 |
NN |
denotes |
Trip13 |
T1429 |
135-136 |
-LRB- |
denotes |
( |
T1430 |
136-143 |
NN |
denotes |
thyroid |
T1431 |
152-160 |
NN |
denotes |
receptor |
T1432 |
144-151 |
NN |
denotes |
hormone |
T1433 |
161-172 |
VBG |
denotes |
interacting |
T1434 |
173-180 |
NN |
denotes |
protein |
T1435 |
181-183 |
CD |
denotes |
13 |
T1436 |
183-184 |
-RRB- |
denotes |
) |
T1437 |
184-186 |
, |
denotes |
, |
T1438 |
194-195 |
DT |
denotes |
a |
T1439 |
196-203 |
NN |
denotes |
protein |
T1440 |
204-208 |
IN |
denotes |
with |
T1441 |
209-218 |
JJ |
denotes |
extensive |
T1442 |
230-238 |
NN |
denotes |
homology |
T1443 |
219-224 |
NN |
denotes |
amino |
T1444 |
225-229 |
NN |
denotes |
acid |
T1445 |
239-241 |
IN |
denotes |
in |
T1446 |
242-249 |
NNS |
denotes |
regions |
T1447 |
250-259 |
JJ |
denotes |
alignable |
T1448 |
260-262 |
IN |
denotes |
to |
T1449 |
263-266 |
DT |
denotes |
the |
T1450 |
282-291 |
NNS |
denotes |
orthologs |
T1451 |
267-272 |
NN |
denotes |
yeast |
T1452 |
273-276 |
CC |
denotes |
and |
T1453 |
277-281 |
NN |
denotes |
worm |
T1454 |
292-293 |
-LRB- |
denotes |
( |
T1455 |
300-302 |
NN |
denotes |
S1 |
T1456 |
293-299 |
NN |
denotes |
Figure |
T1457 |
302-303 |
-RRB- |
denotes |
) |
T1458 |
304-305 |
-LRB- |
denotes |
[ |
T1459 |
305-307 |
CD |
denotes |
12 |
T1460 |
307-308 |
-RRB- |
denotes |
] |
T1461 |
308-309 |
-RRB- |
denotes |
) |
T1462 |
309-310 |
. |
denotes |
. |
T1463 |
310-529 |
sentence |
denotes |
Interestingly, phylogenetic analysis of TRIP13/Pch2p shows that the mammalian protein clusters more closely to plants than it does to the evolutionarily more closely related worms and flies (Figure 1A; see Discussion). |
T1464 |
311-324 |
RB |
denotes |
Interestingly |
T1465 |
364-369 |
VBZ |
denotes |
shows |
T1466 |
324-326 |
, |
denotes |
, |
T1467 |
326-338 |
JJ |
denotes |
phylogenetic |
T1468 |
339-347 |
NN |
denotes |
analysis |
T1469 |
348-350 |
IN |
denotes |
of |
T1470 |
351-357 |
NN |
denotes |
TRIP13 |
T1471 |
358-363 |
NN |
denotes |
Pch2p |
T1472 |
357-358 |
HYPH |
denotes |
/ |
T1473 |
370-374 |
IN |
denotes |
that |
T1474 |
397-405 |
VBZ |
denotes |
clusters |
T1475 |
375-378 |
DT |
denotes |
the |
T1476 |
389-396 |
NN |
denotes |
protein |
T1477 |
379-388 |
JJ |
denotes |
mammalian |
T1478 |
406-410 |
RBR |
denotes |
more |
T1479 |
411-418 |
RB |
denotes |
closely |
T1480 |
419-421 |
IN |
denotes |
to |
T1481 |
422-428 |
NNS |
denotes |
plants |
T1482 |
429-433 |
IN |
denotes |
than |
T1483 |
442-444 |
IN |
denotes |
to |
T1484 |
434-436 |
PRP |
denotes |
it |
T1485 |
437-441 |
VBZ |
denotes |
does |
T1486 |
445-448 |
DT |
denotes |
the |
T1487 |
485-490 |
NNS |
denotes |
worms |
T1488 |
449-463 |
RB |
denotes |
evolutionarily |
T1489 |
477-484 |
VBN |
denotes |
related |
T1490 |
464-468 |
RBR |
denotes |
more |
T1491 |
469-476 |
RB |
denotes |
closely |
T1492 |
491-494 |
CC |
denotes |
and |
T1493 |
495-500 |
NNS |
denotes |
flies |
T1494 |
501-502 |
-LRB- |
denotes |
( |
T1495 |
509-511 |
NN |
denotes |
1A |
T1496 |
502-508 |
NN |
denotes |
Figure |
T1497 |
511-512 |
: |
denotes |
; |
T1498 |
513-516 |
VB |
denotes |
see |
T1499 |
517-527 |
NN |
denotes |
Discussion |
T1500 |
527-528 |
-RRB- |
denotes |
) |
T1501 |
528-529 |
. |
denotes |
. |
T1502 |
529-803 |
sentence |
denotes |
Semi-quantitative reverse-transcriptase PCR (RT-PCR) analysis showed Trip13 mRNA to be expressed in a variety of embryonic and adult tissues, including testis (Figure 1B), consistent with mouse and human EST data summarized in Unigene (http://www.ncbi.nlm.nih.gov/UniGene). |
T1503 |
530-547 |
JJ |
denotes |
Semi-quantitative |
T1504 |
583-591 |
NN |
denotes |
analysis |
T1505 |
548-555 |
NN |
denotes |
reverse |
T1506 |
556-569 |
NN |
denotes |
transcriptase |
T1507 |
555-556 |
HYPH |
denotes |
- |
T1508 |
570-573 |
NN |
denotes |
PCR |
T1509 |
574-575 |
-LRB- |
denotes |
( |
T1510 |
575-577 |
NN |
denotes |
RT |
T1511 |
578-581 |
NN |
denotes |
PCR |
T1512 |
577-578 |
HYPH |
denotes |
- |
T1513 |
581-582 |
-RRB- |
denotes |
) |
T1514 |
592-598 |
VBD |
denotes |
showed |
T1515 |
599-605 |
NN |
denotes |
Trip13 |
T1516 |
606-610 |
NN |
denotes |
mRNA |
T1517 |
617-626 |
VBN |
denotes |
expressed |
T1518 |
611-613 |
TO |
denotes |
to |
T1519 |
614-616 |
VB |
denotes |
be |
T1520 |
627-629 |
IN |
denotes |
in |
T1521 |
630-631 |
DT |
denotes |
a |
T1522 |
632-639 |
NN |
denotes |
variety |
T1523 |
640-642 |
IN |
denotes |
of |
T1524 |
643-652 |
JJ |
denotes |
embryonic |
T1525 |
663-670 |
NNS |
denotes |
tissues |
T1526 |
653-656 |
CC |
denotes |
and |
T1527 |
657-662 |
JJ |
denotes |
adult |
T1528 |
670-672 |
, |
denotes |
, |
T1529 |
672-681 |
VBG |
denotes |
including |
T1530 |
682-688 |
NN |
denotes |
testis |
T1531 |
689-690 |
-LRB- |
denotes |
( |
T1532 |
697-699 |
NN |
denotes |
1B |
T1533 |
690-696 |
NN |
denotes |
Figure |
T1534 |
699-700 |
-RRB- |
denotes |
) |
T1535 |
700-702 |
, |
denotes |
, |
T1536 |
702-712 |
JJ |
denotes |
consistent |
T1537 |
713-717 |
IN |
denotes |
with |
T1538 |
718-723 |
NN |
denotes |
mouse |
T1539 |
734-737 |
NN |
denotes |
EST |
T1540 |
724-727 |
CC |
denotes |
and |
T1541 |
728-733 |
JJ |
denotes |
human |
T1542 |
738-742 |
NNS |
denotes |
data |
T1543 |
743-753 |
VBN |
denotes |
summarized |
T1544 |
754-756 |
IN |
denotes |
in |
T1545 |
757-764 |
NNP |
denotes |
Unigene |
T1546 |
765-766 |
-LRB- |
denotes |
( |
T1547 |
766-801 |
NN |
denotes |
http://www.ncbi.nlm.nih.gov/UniGene |
T1548 |
801-802 |
-RRB- |
denotes |
) |
T1549 |
802-803 |
. |
denotes |
. |
T1550 |
803-864 |
sentence |
denotes |
It is also highly expressed in human and mouse oocytes [17]. |
T1551 |
804-806 |
PRP |
denotes |
It |
T1552 |
822-831 |
VBN |
denotes |
expressed |
T1553 |
807-809 |
VBZ |
denotes |
is |
T1554 |
810-814 |
RB |
denotes |
also |
T1555 |
815-821 |
RB |
denotes |
highly |
T1556 |
832-834 |
IN |
denotes |
in |
T1557 |
835-840 |
JJ |
denotes |
human |
T1558 |
845-850 |
NN |
denotes |
mouse |
T1559 |
841-844 |
CC |
denotes |
and |
T1560 |
851-858 |
NNS |
denotes |
oocytes |
T1561 |
859-860 |
-LRB- |
denotes |
[ |
T1562 |
860-862 |
CD |
denotes |
17 |
T1563 |
862-863 |
-RRB- |
denotes |
] |
T1564 |
863-864 |
. |
denotes |
. |
T10169 |
875-878 |
DT |
denotes |
The |
T10170 |
890-898 |
NN |
denotes |
Ortholog |
T10171 |
879-884 |
NN |
denotes |
Mouse |
T10172 |
885-889 |
NN |
denotes |
PCH2 |
T10173 |
899-905 |
NN |
denotes |
TRIP13 |
T10174 |
906-909 |
CC |
denotes |
and |
T10175 |
910-920 |
NN |
denotes |
Expression |
T10176 |
921-923 |
IN |
denotes |
in |
T10177 |
924-928 |
JJ |
denotes |
Wild |
T10178 |
929-933 |
NN |
denotes |
Type |
T10179 |
934-937 |
CC |
denotes |
and |
T10180 |
938-944 |
NN |
denotes |
Mutant |
T10181 |
944-1001 |
sentence |
denotes |
(A) Phylogenetic tree of presumed PCH2/TRIP13 orthologs. |
T10182 |
945-946 |
-LRB- |
denotes |
( |
T10183 |
946-947 |
LS |
denotes |
A |
T10184 |
962-966 |
NN |
denotes |
tree |
T10185 |
947-948 |
-RRB- |
denotes |
) |
T10186 |
949-961 |
JJ |
denotes |
Phylogenetic |
T10187 |
967-969 |
IN |
denotes |
of |
T10188 |
970-978 |
JJ |
denotes |
presumed |
T10189 |
991-1000 |
NNS |
denotes |
orthologs |
T10190 |
979-983 |
NN |
denotes |
PCH2 |
T10191 |
984-990 |
NN |
denotes |
TRIP13 |
T10192 |
983-984 |
HYPH |
denotes |
/ |
T10193 |
1000-1001 |
. |
denotes |
. |
T10194 |
1001-1082 |
sentence |
denotes |
The database sequence accession number of each protein is presented in Table S1. |
T10195 |
1002-1005 |
DT |
denotes |
The |
T10196 |
1034-1040 |
NN |
denotes |
number |
T10197 |
1006-1014 |
NN |
denotes |
database |
T10198 |
1015-1023 |
NN |
denotes |
sequence |
T10199 |
1024-1033 |
NN |
denotes |
accession |
T10200 |
1060-1069 |
VBN |
denotes |
presented |
T10201 |
1041-1043 |
IN |
denotes |
of |
T10202 |
1044-1048 |
DT |
denotes |
each |
T10203 |
1049-1056 |
NN |
denotes |
protein |
T10204 |
1057-1059 |
VBZ |
denotes |
is |
T10205 |
1070-1072 |
IN |
denotes |
in |
T10206 |
1073-1078 |
NN |
denotes |
Table |
T10207 |
1079-1081 |
NN |
denotes |
S1 |
T10208 |
1081-1082 |
. |
denotes |
. |
T10209 |
1082-1146 |
sentence |
denotes |
Numbers shown are bootstrap values (see Materials and Methods). |
T10210 |
1083-1090 |
NNS |
denotes |
Numbers |
T10211 |
1097-1100 |
VBP |
denotes |
are |
T10212 |
1091-1096 |
VBN |
denotes |
shown |
T10213 |
1101-1110 |
NN |
denotes |
bootstrap |
T10214 |
1111-1117 |
NNS |
denotes |
values |
T10215 |
1118-1119 |
-LRB- |
denotes |
( |
T10216 |
1119-1122 |
VB |
denotes |
see |
T10217 |
1123-1132 |
NNS |
denotes |
Materials |
T10218 |
1133-1136 |
CC |
denotes |
and |
T10219 |
1137-1144 |
NNS |
denotes |
Methods |
T10220 |
1144-1145 |
-RRB- |
denotes |
) |
T10221 |
1145-1146 |
. |
denotes |
. |
T10222 |
1146-1331 |
sentence |
denotes |
The clustering was the same regardless of whether the whole entire AA sequence or trimmed sequences (where regions showing little conservation were removed) were used for the analysis. |
T10223 |
1147-1150 |
DT |
denotes |
The |
T10224 |
1151-1161 |
NN |
denotes |
clustering |
T10225 |
1162-1165 |
VBD |
denotes |
was |
T10226 |
1166-1169 |
DT |
denotes |
the |
T10227 |
1170-1174 |
JJ |
denotes |
same |
T10228 |
1175-1185 |
RB |
denotes |
regardless |
T10229 |
1186-1188 |
IN |
denotes |
of |
T10230 |
1189-1196 |
IN |
denotes |
whether |
T10231 |
1309-1313 |
VBN |
denotes |
used |
T10232 |
1197-1200 |
DT |
denotes |
the |
T10233 |
1217-1225 |
NN |
denotes |
sequence |
T10234 |
1201-1206 |
JJ |
denotes |
whole |
T10235 |
1207-1213 |
JJ |
denotes |
entire |
T10236 |
1214-1216 |
NN |
denotes |
AA |
T10237 |
1226-1228 |
CC |
denotes |
or |
T10238 |
1229-1236 |
VBN |
denotes |
trimmed |
T10239 |
1237-1246 |
NNS |
denotes |
sequences |
T10240 |
1247-1248 |
-LRB- |
denotes |
( |
T10241 |
1248-1253 |
WRB |
denotes |
where |
T10242 |
1295-1302 |
VBN |
denotes |
removed |
T10243 |
1254-1261 |
NNS |
denotes |
regions |
T10244 |
1262-1269 |
VBG |
denotes |
showing |
T10245 |
1270-1276 |
JJ |
denotes |
little |
T10246 |
1277-1289 |
NN |
denotes |
conservation |
T10247 |
1290-1294 |
VBD |
denotes |
were |
T10248 |
1302-1303 |
-RRB- |
denotes |
) |
T10249 |
1304-1308 |
VBD |
denotes |
were |
T10250 |
1314-1317 |
IN |
denotes |
for |
T10251 |
1318-1321 |
DT |
denotes |
the |
T10252 |
1322-1330 |
NN |
denotes |
analysis |
T10253 |
1330-1331 |
. |
denotes |
. |
T10254 |
1331-1468 |
sentence |
denotes |
Major eukaryotic groups are indicated in color, with deuterostomia in blue, plants in green, protostomia in purple, and fungi in maroon. |
T10255 |
1332-1337 |
JJ |
denotes |
Major |
T10256 |
1349-1355 |
NNS |
denotes |
groups |
T10257 |
1338-1348 |
JJ |
denotes |
eukaryotic |
T10258 |
1360-1369 |
VBN |
denotes |
indicated |
T10259 |
1356-1359 |
VBP |
denotes |
are |
T10260 |
1370-1372 |
IN |
denotes |
in |
T10261 |
1373-1378 |
NN |
denotes |
color |
T10262 |
1378-1380 |
, |
denotes |
, |
T10263 |
1380-1384 |
IN |
denotes |
with |
T10264 |
1399-1401 |
IN |
denotes |
in |
T10265 |
1385-1398 |
NN |
denotes |
deuterostomia |
T10266 |
1402-1406 |
NN |
denotes |
blue |
T10267 |
1406-1408 |
, |
denotes |
, |
T10268 |
1408-1414 |
NNS |
denotes |
plants |
T10269 |
1415-1417 |
IN |
denotes |
in |
T10270 |
1418-1423 |
NN |
denotes |
green |
T10271 |
1423-1425 |
, |
denotes |
, |
T10272 |
1425-1436 |
NN |
denotes |
protostomia |
T10273 |
1437-1439 |
IN |
denotes |
in |
T10274 |
1440-1446 |
NN |
denotes |
purple |
T10275 |
1446-1448 |
, |
denotes |
, |
T10276 |
1448-1451 |
CC |
denotes |
and |
T10277 |
1452-1457 |
NNS |
denotes |
fungi |
T10278 |
1458-1460 |
IN |
denotes |
in |
T10279 |
1461-1467 |
NN |
denotes |
maroon |
T10280 |
1467-1468 |
. |
denotes |
. |
T10281 |
1468-1689 |
sentence |
denotes |
(B) Amplification products of cDNA from the following tissues: 1, heart; 2, brain; 3, spleen; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; 8, testis; 9, E7 embryo; 10, E11 embryo; 11, E15 embryo; and 12, E17 embryo. |
T10282 |
1469-1470 |
-LRB- |
denotes |
( |
T10283 |
1470-1471 |
LS |
denotes |
B |
T10284 |
1487-1495 |
NNS |
denotes |
products |
T10285 |
1471-1472 |
-RRB- |
denotes |
) |
T10286 |
1473-1486 |
NN |
denotes |
Amplification |
T10287 |
1496-1498 |
IN |
denotes |
of |
T10288 |
1499-1503 |
NN |
denotes |
cDNA |
T10289 |
1504-1508 |
IN |
denotes |
from |
T10290 |
1509-1512 |
DT |
denotes |
the |
T10291 |
1523-1530 |
NNS |
denotes |
tissues |
T10292 |
1513-1522 |
JJ |
denotes |
following |
T10293 |
1530-1532 |
: |
denotes |
: |
T10294 |
1532-1533 |
LS |
denotes |
1 |
T10295 |
1535-1540 |
NN |
denotes |
heart |
T10296 |
1533-1535 |
, |
denotes |
, |
T10297 |
1540-1541 |
: |
denotes |
; |
T10298 |
1542-1543 |
LS |
denotes |
2 |
T10299 |
1545-1550 |
NN |
denotes |
brain |
T10300 |
1543-1545 |
, |
denotes |
, |
T10301 |
1550-1551 |
: |
denotes |
; |
T10302 |
1552-1553 |
LS |
denotes |
3 |
T10303 |
1555-1561 |
NN |
denotes |
spleen |
T10304 |
1553-1555 |
, |
denotes |
, |
T10305 |
1561-1562 |
: |
denotes |
; |
T10306 |
1563-1564 |
LS |
denotes |
4 |
T10307 |
1566-1570 |
NN |
denotes |
lung |
T10308 |
1564-1566 |
, |
denotes |
, |
T10309 |
1570-1571 |
: |
denotes |
; |
T10310 |
1572-1573 |
LS |
denotes |
5 |
T10311 |
1575-1580 |
NN |
denotes |
liver |
T10312 |
1573-1575 |
, |
denotes |
, |
T10313 |
1580-1581 |
: |
denotes |
; |
T10314 |
1582-1583 |
LS |
denotes |
6 |
T10315 |
1594-1600 |
NN |
denotes |
muscle |
T10316 |
1583-1585 |
, |
denotes |
, |
T10317 |
1585-1593 |
JJ |
denotes |
skeletal |
T10318 |
1600-1601 |
: |
denotes |
; |
T10319 |
1602-1603 |
LS |
denotes |
7 |
T10320 |
1605-1611 |
NN |
denotes |
kidney |
T10321 |
1603-1605 |
, |
denotes |
, |
T10322 |
1611-1612 |
: |
denotes |
; |
T10323 |
1613-1614 |
LS |
denotes |
8 |
T10324 |
1616-1622 |
NN |
denotes |
testis |
T10325 |
1614-1616 |
, |
denotes |
, |
T10326 |
1622-1623 |
: |
denotes |
; |
T10327 |
1624-1625 |
LS |
denotes |
9 |
T10328 |
1630-1636 |
NN |
denotes |
embryo |
T10329 |
1625-1627 |
, |
denotes |
, |
T10330 |
1627-1629 |
NN |
denotes |
E7 |
T10331 |
1636-1637 |
: |
denotes |
; |
T10332 |
1638-1640 |
LS |
denotes |
10 |
T10333 |
1646-1652 |
NN |
denotes |
embryo |
T10334 |
1640-1642 |
, |
denotes |
, |
T10335 |
1642-1645 |
NN |
denotes |
E11 |
T10336 |
1652-1653 |
: |
denotes |
; |
T10337 |
1654-1656 |
LS |
denotes |
11 |
T10338 |
1662-1668 |
NN |
denotes |
embryo |
T10339 |
1656-1658 |
, |
denotes |
, |
T10340 |
1658-1661 |
NN |
denotes |
E15 |
T10341 |
1668-1669 |
: |
denotes |
; |
T10342 |
1670-1673 |
CC |
denotes |
and |
T10343 |
1674-1676 |
LS |
denotes |
12 |
T10344 |
1682-1688 |
NN |
denotes |
embryo |
T10345 |
1676-1678 |
, |
denotes |
, |
T10346 |
1678-1681 |
NN |
denotes |
E17 |
T10347 |
1688-1689 |
. |
denotes |
. |
T10348 |
1689-1765 |
sentence |
denotes |
(C) Intron–exon structure of TRIP13 and insertion site of gene-trap vector. |
T10349 |
1690-1691 |
-LRB- |
denotes |
( |
T10350 |
1691-1692 |
LS |
denotes |
C |
T10351 |
1706-1715 |
NN |
denotes |
structure |
T10352 |
1692-1693 |
-RRB- |
denotes |
) |
T10353 |
1694-1700 |
NN |
denotes |
Intron |
T10354 |
1701-1705 |
NN |
denotes |
exon |
T10355 |
1700-1701 |
HYPH |
denotes |
– |
T10356 |
1716-1718 |
IN |
denotes |
of |
T10357 |
1719-1725 |
NN |
denotes |
TRIP13 |
T10358 |
1726-1729 |
CC |
denotes |
and |
T10359 |
1730-1739 |
NN |
denotes |
insertion |
T10360 |
1740-1744 |
NN |
denotes |
site |
T10361 |
1745-1747 |
IN |
denotes |
of |
T10362 |
1748-1752 |
NN |
denotes |
gene |
T10363 |
1753-1757 |
NN |
denotes |
trap |
T10364 |
1752-1753 |
HYPH |
denotes |
- |
T10365 |
1758-1764 |
NN |
denotes |
vector |
T10366 |
1764-1765 |
. |
denotes |
. |
T10367 |
1765-1853 |
sentence |
denotes |
See Materials and Methods for details on how the precise insertion site was identified. |
T10368 |
1766-1769 |
VB |
denotes |
See |
T10369 |
1770-1779 |
NNS |
denotes |
Materials |
T10370 |
1780-1783 |
CC |
denotes |
and |
T10371 |
1784-1791 |
NNS |
denotes |
Methods |
T10372 |
1792-1795 |
IN |
denotes |
for |
T10373 |
1796-1803 |
NNS |
denotes |
details |
T10374 |
1804-1806 |
IN |
denotes |
on |
T10375 |
1807-1810 |
WRB |
denotes |
how |
T10376 |
1842-1852 |
VBN |
denotes |
identified |
T10377 |
1811-1814 |
DT |
denotes |
the |
T10378 |
1833-1837 |
NN |
denotes |
site |
T10379 |
1815-1822 |
JJ |
denotes |
precise |
T10380 |
1823-1832 |
NN |
denotes |
insertion |
T10381 |
1838-1841 |
VBD |
denotes |
was |
T10382 |
1852-1853 |
. |
denotes |
. |
T10383 |
1853-1916 |
sentence |
denotes |
(D) RT-PCR of Trip13 and a control gene Med31 from testis RNA. |
T10384 |
1854-1855 |
-LRB- |
denotes |
( |
T10385 |
1855-1856 |
LS |
denotes |
D |
T10386 |
1861-1864 |
NN |
denotes |
PCR |
T10387 |
1856-1857 |
-RRB- |
denotes |
) |
T10388 |
1858-1860 |
NN |
denotes |
RT |
T10389 |
1860-1861 |
HYPH |
denotes |
- |
T10390 |
1865-1867 |
IN |
denotes |
of |
T10391 |
1868-1874 |
NN |
denotes |
Trip13 |
T10392 |
1875-1878 |
CC |
denotes |
and |
T10393 |
1879-1880 |
DT |
denotes |
a |
T10394 |
1894-1899 |
NN |
denotes |
Med31 |
T10395 |
1881-1888 |
NN |
denotes |
control |
T10396 |
1889-1893 |
NN |
denotes |
gene |
T10397 |
1900-1904 |
IN |
denotes |
from |
T10398 |
1905-1911 |
NN |
denotes |
testis |
T10399 |
1912-1915 |
NN |
denotes |
RNA |
T10400 |
1915-1916 |
. |
denotes |
. |
T10401 |
1916-2005 |
sentence |
denotes |
The Trip13 primers are situated in the first and last exons (see Materials and Methods). |
T10402 |
1917-1920 |
DT |
denotes |
The |
T10403 |
1928-1935 |
NNS |
denotes |
primers |
T10404 |
1921-1927 |
NN |
denotes |
Trip13 |
T10405 |
1940-1948 |
VBN |
denotes |
situated |
T10406 |
1936-1939 |
VBP |
denotes |
are |
T10407 |
1949-1951 |
IN |
denotes |
in |
T10408 |
1952-1955 |
DT |
denotes |
the |
T10409 |
1971-1976 |
NNS |
denotes |
exons |
T10410 |
1956-1961 |
JJ |
denotes |
first |
T10411 |
1962-1965 |
CC |
denotes |
and |
T10412 |
1966-1970 |
JJ |
denotes |
last |
T10413 |
1977-1978 |
-LRB- |
denotes |
( |
T10414 |
1978-1981 |
VB |
denotes |
see |
T10415 |
1982-1991 |
NNS |
denotes |
Materials |
T10416 |
1992-1995 |
CC |
denotes |
and |
T10417 |
1996-2003 |
NNS |
denotes |
Methods |
T10418 |
2003-2004 |
-RRB- |
denotes |
) |
T10419 |
2004-2005 |
. |
denotes |
. |
T10420 |
2005-2076 |
sentence |
denotes |
(E) Western blot analysis of testis protein with anti-TRIP13 antibody. |
T10421 |
2006-2007 |
-LRB- |
denotes |
( |
T10422 |
2007-2008 |
LS |
denotes |
E |
T10423 |
2023-2031 |
NN |
denotes |
analysis |
T10424 |
2008-2009 |
-RRB- |
denotes |
) |
T10425 |
2010-2017 |
NNP |
denotes |
Western |
T10426 |
2018-2022 |
NN |
denotes |
blot |
T10427 |
2032-2034 |
IN |
denotes |
of |
T10428 |
2035-2041 |
NN |
denotes |
testis |
T10429 |
2042-2049 |
NN |
denotes |
protein |
T10430 |
2050-2054 |
IN |
denotes |
with |
T10431 |
2055-2066 |
JJ |
denotes |
anti-TRIP13 |
T10432 |
2067-2075 |
NN |
denotes |
antibody |
T10433 |
2075-2076 |
. |
denotes |
. |
T10434 |
2076-2154 |
sentence |
denotes |
The blot was later probed with anti-alpha tubulin actin as a loading control. |
T10435 |
2077-2080 |
DT |
denotes |
The |
T10436 |
2081-2085 |
NN |
denotes |
blot |
T10437 |
2096-2102 |
VBN |
denotes |
probed |
T10438 |
2086-2089 |
VBD |
denotes |
was |
T10439 |
2090-2095 |
RB |
denotes |
later |
T10440 |
2103-2107 |
IN |
denotes |
with |
T10441 |
2108-2118 |
JJ |
denotes |
anti-alpha |
T10442 |
2127-2132 |
NN |
denotes |
actin |
T10443 |
2119-2126 |
NN |
denotes |
tubulin |
T10444 |
2133-2135 |
IN |
denotes |
as |
T10445 |
2136-2137 |
DT |
denotes |
a |
T10446 |
2146-2153 |
NN |
denotes |
control |
T10447 |
2138-2145 |
NN |
denotes |
loading |
T10448 |
2153-2154 |
. |
denotes |
. |
T10449 |
2154-2194 |
sentence |
denotes |
The expected TRIP13 protein is ∼48 KDa. |
T10450 |
2155-2158 |
DT |
denotes |
The |
T10451 |
2175-2182 |
NN |
denotes |
protein |
T10452 |
2159-2167 |
JJ |
denotes |
expected |
T10453 |
2168-2174 |
NN |
denotes |
TRIP13 |
T10454 |
2183-2185 |
VBZ |
denotes |
is |
T10455 |
2186-2187 |
SYM |
denotes |
∼ |
T10456 |
2187-2189 |
CD |
denotes |
48 |
T10457 |
2190-2193 |
NN |
denotes |
KDa |
T10458 |
2193-2194 |
. |
denotes |
. |
T10459 |
2194-2232 |
sentence |
denotes |
(F) Localization of TRIP13 in testes. |
T10460 |
2195-2196 |
-LRB- |
denotes |
( |
T10461 |
2196-2197 |
LS |
denotes |
F |
T10462 |
2199-2211 |
NN |
denotes |
Localization |
T10463 |
2197-2198 |
-RRB- |
denotes |
) |
T10464 |
2212-2214 |
IN |
denotes |
of |
T10465 |
2215-2221 |
NN |
denotes |
TRIP13 |
T10466 |
2222-2224 |
IN |
denotes |
in |
T10467 |
2225-2231 |
NNS |
denotes |
testes |
T10468 |
2231-2232 |
. |
denotes |
. |
T10469 |
2232-2394 |
sentence |
denotes |
Wild-type (top) and mutant (bottom) testis sections were probed with chicken anti-TRIP13, and detected with HRP-conjugated anti-chicken IgG (brown/red staining). |
T10470 |
2233-2237 |
JJ |
denotes |
Wild |
T10471 |
2238-2242 |
NN |
denotes |
type |
T10472 |
2237-2238 |
HYPH |
denotes |
- |
T10473 |
2269-2275 |
NN |
denotes |
testis |
T10474 |
2243-2244 |
-LRB- |
denotes |
( |
T10475 |
2244-2247 |
JJ |
denotes |
top |
T10476 |
2247-2248 |
-RRB- |
denotes |
) |
T10477 |
2249-2252 |
CC |
denotes |
and |
T10478 |
2253-2259 |
NN |
denotes |
mutant |
T10479 |
2260-2261 |
-LRB- |
denotes |
( |
T10480 |
2261-2267 |
JJ |
denotes |
bottom |
T10481 |
2267-2268 |
-RRB- |
denotes |
) |
T10482 |
2276-2284 |
NNS |
denotes |
sections |
T10483 |
2290-2296 |
VBN |
denotes |
probed |
T10484 |
2285-2289 |
VBD |
denotes |
were |
T10485 |
2297-2301 |
IN |
denotes |
with |
T10486 |
2302-2309 |
NN |
denotes |
chicken |
T10487 |
2310-2321 |
JJ |
denotes |
anti-TRIP13 |
T10488 |
2321-2323 |
, |
denotes |
, |
T10489 |
2323-2326 |
CC |
denotes |
and |
T10490 |
2327-2335 |
VBN |
denotes |
detected |
T10491 |
2336-2340 |
IN |
denotes |
with |
T10492 |
2341-2344 |
NN |
denotes |
HRP |
T10493 |
2345-2355 |
VBN |
denotes |
conjugated |
T10494 |
2344-2345 |
HYPH |
denotes |
- |
T10495 |
2369-2372 |
NN |
denotes |
IgG |
T10496 |
2356-2368 |
JJ |
denotes |
anti-chicken |
T10497 |
2373-2374 |
-LRB- |
denotes |
( |
T10498 |
2384-2392 |
NN |
denotes |
staining |
T10499 |
2374-2379 |
JJ |
denotes |
brown |
T10500 |
2380-2383 |
JJ |
denotes |
red |
T10501 |
2379-2380 |
HYPH |
denotes |
/ |
T10502 |
2392-2393 |
-RRB- |
denotes |
) |
T10503 |
2393-2394 |
. |
denotes |
. |
T10504 |
2394-2587 |
sentence |
denotes |
Expression in WT was most prominent in the nuclei of Type B spermatogonia (Sg), leptotene spermatocytes (LS), and early pachytene spermatocytes (PS), but not late pachytene spermatocytes (LP). |
T10505 |
2395-2405 |
NN |
denotes |
Expression |
T10506 |
2412-2415 |
VBD |
denotes |
was |
T10507 |
2406-2408 |
IN |
denotes |
in |
T10508 |
2409-2411 |
NN |
denotes |
WT |
T10509 |
2416-2420 |
RBS |
denotes |
most |
T10510 |
2421-2430 |
JJ |
denotes |
prominent |
T10511 |
2431-2433 |
IN |
denotes |
in |
T10512 |
2434-2437 |
DT |
denotes |
the |
T10513 |
2438-2444 |
NNS |
denotes |
nuclei |
T10514 |
2445-2447 |
IN |
denotes |
of |
T10515 |
2448-2452 |
NN |
denotes |
Type |
T10516 |
2453-2454 |
NN |
denotes |
B |
T10517 |
2455-2468 |
NNS |
denotes |
spermatogonia |
T10518 |
2469-2470 |
-LRB- |
denotes |
( |
T10519 |
2470-2472 |
NN |
denotes |
Sg |
T10520 |
2472-2473 |
-RRB- |
denotes |
) |
T10521 |
2473-2475 |
, |
denotes |
, |
T10522 |
2475-2484 |
NN |
denotes |
leptotene |
T10523 |
2485-2498 |
NNS |
denotes |
spermatocytes |
T10524 |
2499-2500 |
-LRB- |
denotes |
( |
T10525 |
2500-2502 |
NNS |
denotes |
LS |
T10526 |
2502-2503 |
-RRB- |
denotes |
) |
T10527 |
2503-2505 |
, |
denotes |
, |
T10528 |
2505-2508 |
CC |
denotes |
and |
T10529 |
2509-2514 |
JJ |
denotes |
early |
T10530 |
2525-2538 |
NNS |
denotes |
spermatocytes |
T10531 |
2515-2524 |
NN |
denotes |
pachytene |
T10532 |
2539-2540 |
-LRB- |
denotes |
( |
T10533 |
2540-2542 |
NN |
denotes |
PS |
T10534 |
2542-2543 |
-RRB- |
denotes |
) |
T10535 |
2543-2545 |
, |
denotes |
, |
T10536 |
2545-2548 |
CC |
denotes |
but |
T10537 |
2549-2552 |
RB |
denotes |
not |
T10538 |
2553-2557 |
JJ |
denotes |
late |
T10539 |
2568-2581 |
NNS |
denotes |
spermatocytes |
T10540 |
2558-2567 |
NN |
denotes |
pachytene |
T10541 |
2582-2583 |
-LRB- |
denotes |
( |
T10542 |
2583-2585 |
NN |
denotes |
LP |
T10543 |
2585-2586 |
-RRB- |
denotes |
) |
T10544 |
2586-2587 |
. |
denotes |
. |
T10545 |
2587-2695 |
sentence |
denotes |
No nuclear staining was seen in mutant testis sections, although reddish cytoplasmic background is present. |
T10546 |
2588-2590 |
DT |
denotes |
No |
T10547 |
2599-2607 |
NN |
denotes |
staining |
T10548 |
2591-2598 |
JJ |
denotes |
nuclear |
T10549 |
2612-2616 |
VBN |
denotes |
seen |
T10550 |
2608-2611 |
VBD |
denotes |
was |
T10551 |
2617-2619 |
IN |
denotes |
in |
T10552 |
2620-2626 |
NN |
denotes |
mutant |
T10553 |
2634-2642 |
NNS |
denotes |
sections |
T10554 |
2627-2633 |
NN |
denotes |
testis |
T10555 |
2642-2644 |
, |
denotes |
, |
T10556 |
2644-2652 |
IN |
denotes |
although |
T10557 |
2684-2686 |
VBZ |
denotes |
is |
T10558 |
2653-2660 |
JJ |
denotes |
reddish |
T10559 |
2673-2683 |
NN |
denotes |
background |
T10560 |
2661-2672 |
JJ |
denotes |
cytoplasmic |
T10561 |
2687-2694 |
JJ |
denotes |
present |
T10562 |
2694-2695 |
. |
denotes |
. |
T10563 |
2695-2812 |
sentence |
denotes |
Identification of cell types was judged in part by estimating the epithelial stage of the tubules as described [67]. |
T10564 |
2696-2710 |
NN |
denotes |
Identification |
T10565 |
2729-2735 |
VBN |
denotes |
judged |
T10566 |
2711-2713 |
IN |
denotes |
of |
T10567 |
2714-2718 |
NN |
denotes |
cell |
T10568 |
2719-2724 |
NNS |
denotes |
types |
T10569 |
2725-2728 |
VBD |
denotes |
was |
T10570 |
2736-2738 |
IN |
denotes |
in |
T10571 |
2739-2743 |
JJ |
denotes |
part |
T10572 |
2744-2746 |
IN |
denotes |
by |
T10573 |
2747-2757 |
VBG |
denotes |
estimating |
T10574 |
2758-2761 |
DT |
denotes |
the |
T10575 |
2773-2778 |
NN |
denotes |
stage |
T10576 |
2762-2772 |
JJ |
denotes |
epithelial |
T10577 |
2779-2781 |
IN |
denotes |
of |
T10578 |
2782-2785 |
DT |
denotes |
the |
T10579 |
2786-2793 |
NNS |
denotes |
tubules |
T10580 |
2794-2796 |
IN |
denotes |
as |
T10581 |
2797-2806 |
VBN |
denotes |
described |
T10582 |
2807-2808 |
-LRB- |
denotes |
[ |
T10583 |
2808-2810 |
CD |
denotes |
67 |
T10584 |
2810-2811 |
-RRB- |
denotes |
] |
T10585 |
2811-2812 |
. |
denotes |
. |
T10586 |
2812-2869 |
sentence |
denotes |
(G) TRIP13 localization in surface-spread spermatocytes. |
T10587 |
2813-2814 |
-LRB- |
denotes |
( |
T10588 |
2814-2815 |
LS |
denotes |
G |
T10589 |
2824-2836 |
NN |
denotes |
localization |
T10590 |
2815-2816 |
-RRB- |
denotes |
) |
T10591 |
2817-2823 |
NN |
denotes |
TRIP13 |
T10592 |
2837-2839 |
IN |
denotes |
in |
T10593 |
2840-2847 |
NN |
denotes |
surface |
T10594 |
2848-2854 |
NN |
denotes |
spread |
T10595 |
2847-2848 |
HYPH |
denotes |
- |
T10596 |
2855-2868 |
NNS |
denotes |
spermatocytes |
T10597 |
2868-2869 |
. |
denotes |
. |
T10598 |
2869-2937 |
sentence |
denotes |
Preparations were immunolabeled with anti-SYCP3 (S) and TRIP13 (T). |
T10599 |
2870-2882 |
NNS |
denotes |
Preparations |
T10600 |
2888-2901 |
VBN |
denotes |
immunolabeled |
T10601 |
2883-2887 |
VBD |
denotes |
were |
T10602 |
2902-2906 |
IN |
denotes |
with |
T10603 |
2907-2917 |
JJ |
denotes |
anti-SYCP3 |
T10604 |
2918-2919 |
-LRB- |
denotes |
( |
T10605 |
2919-2920 |
NN |
denotes |
S |
T10606 |
2920-2921 |
-RRB- |
denotes |
) |
T10607 |
2922-2925 |
CC |
denotes |
and |
T10608 |
2926-2932 |
NN |
denotes |
TRIP13 |
T10609 |
2933-2934 |
-LRB- |
denotes |
( |
T10610 |
2934-2935 |
NN |
denotes |
T |
T10611 |
2935-2936 |
-RRB- |
denotes |
) |
T10612 |
2936-2937 |
. |
denotes |
. |
T10613 |
2937-3053 |
sentence |
denotes |
Both individual and merged images are shown for leptotene (Lep), zygotene (Zyg), and pachytene (Pac) spermatocytes. |
T10614 |
2938-2942 |
CC |
denotes |
Both |
T10615 |
2943-2953 |
JJ |
denotes |
individual |
T10616 |
2965-2971 |
NNS |
denotes |
images |
T10617 |
2954-2957 |
CC |
denotes |
and |
T10618 |
2958-2964 |
VBN |
denotes |
merged |
T10619 |
2976-2981 |
VBN |
denotes |
shown |
T10620 |
2972-2975 |
VBP |
denotes |
are |
T10621 |
2982-2985 |
IN |
denotes |
for |
T10622 |
2986-2995 |
NN |
denotes |
leptotene |
T10623 |
3039-3052 |
NNS |
denotes |
spermatocytes |
T10624 |
2996-2997 |
-LRB- |
denotes |
( |
T10625 |
2997-3000 |
NN |
denotes |
Lep |
T10626 |
3000-3001 |
-RRB- |
denotes |
) |
T10627 |
3001-3003 |
, |
denotes |
, |
T10628 |
3003-3011 |
NN |
denotes |
zygotene |
T10629 |
3012-3013 |
-LRB- |
denotes |
( |
T10630 |
3013-3016 |
NN |
denotes |
Zyg |
T10631 |
3016-3017 |
-RRB- |
denotes |
) |
T10632 |
3017-3019 |
, |
denotes |
, |
T10633 |
3019-3022 |
CC |
denotes |
and |
T10634 |
3023-3032 |
NN |
denotes |
pachytene |
T10635 |
3033-3034 |
-LRB- |
denotes |
( |
T10636 |
3034-3037 |
NN |
denotes |
Pac |
T10637 |
3037-3038 |
-RRB- |
denotes |
) |
T10638 |
3052-3053 |
. |
denotes |
. |
T10639 |
3053-3096 |
sentence |
denotes |
Nuclear staining was absent in the mutant. |
T10640 |
3054-3061 |
JJ |
denotes |
Nuclear |
T10641 |
3062-3070 |
NN |
denotes |
staining |
T10642 |
3071-3074 |
VBD |
denotes |
was |
T10643 |
3075-3081 |
JJ |
denotes |
absent |
T10644 |
3082-3084 |
IN |
denotes |
in |
T10645 |
3085-3088 |
DT |
denotes |
the |
T10646 |
3089-3095 |
NN |
denotes |
mutant |
T10647 |
3095-3096 |
. |
denotes |
. |
R921 |
T1452 |
T1451 |
cc |
and,yeast |
R922 |
T1453 |
T1451 |
conj |
worm,yeast |
R923 |
T1454 |
T1455 |
punct |
(,S1 |
R924 |
T1455 |
T1424 |
parataxis |
S1,encodes |
R925 |
T1456 |
T1455 |
compound |
Figure,S1 |
R879 |
T1407 |
T1408 |
nsubj |
Trip13,Is |
R880 |
T1409 |
T1410 |
det |
a,Ortholog |
R881 |
T1410 |
T1408 |
attr |
Ortholog,Is |
R882 |
T1411 |
T1412 |
advmod |
Widely,Expressed |
R883 |
T1412 |
T1410 |
amod |
Expressed,Ortholog |
R884 |
T1413 |
T1410 |
amod |
Mammalian,Ortholog |
R885 |
T1414 |
T1410 |
prep |
of,Ortholog |
R886 |
T1415 |
T1414 |
pobj |
PCH2,of |
R887 |
T1416 |
T1410 |
prep |
with,Ortholog |
R888 |
T1417 |
T1418 |
amod |
Unusual,Relationships |
R889 |
T1418 |
T1416 |
pobj |
Relationships,with |
R890 |
T1419 |
T1418 |
amod |
Phylogenetic,Relationships |
R891 |
T1421 |
T1422 |
det |
The,ortholog |
R892 |
T1422 |
T1424 |
nsubj |
ortholog,encodes |
R893 |
T1423 |
T1422 |
amod |
mammalian,ortholog |
R894 |
T1425 |
T1422 |
prep |
of,ortholog |
R895 |
T1426 |
T1425 |
pobj |
PCH2,of |
R896 |
T1427 |
T1422 |
punct |
", ",ortholog |
R897 |
T1428 |
T1422 |
appos |
Trip13,ortholog |
R898 |
T1429 |
T1428 |
punct |
(,Trip13 |
R899 |
T1430 |
T1431 |
compound |
thyroid,receptor |
R900 |
T1431 |
T1433 |
npadvmod |
receptor,interacting |
R901 |
T1432 |
T1431 |
compound |
hormone,receptor |
R902 |
T1433 |
T1434 |
amod |
interacting,protein |
R903 |
T1434 |
T1428 |
appos |
protein,Trip13 |
R904 |
T1435 |
T1434 |
nummod |
13,protein |
R905 |
T1436 |
T1424 |
punct |
),encodes |
R906 |
T1437 |
T1424 |
punct |
", ",encodes |
R907 |
T1438 |
T1439 |
det |
a,protein |
R908 |
T1439 |
T1424 |
dobj |
protein,encodes |
R909 |
T1440 |
T1439 |
prep |
with,protein |
R910 |
T1441 |
T1442 |
amod |
extensive,homology |
R911 |
T1442 |
T1440 |
pobj |
homology,with |
R912 |
T1443 |
T1444 |
compound |
amino,acid |
R913 |
T1444 |
T1442 |
compound |
acid,homology |
R914 |
T1445 |
T1442 |
prep |
in,homology |
R915 |
T1446 |
T1445 |
pobj |
regions,in |
R916 |
T1447 |
T1446 |
amod |
alignable,regions |
R917 |
T1448 |
T1447 |
prep |
to,alignable |
R918 |
T1449 |
T1450 |
det |
the,orthologs |
R919 |
T1450 |
T1448 |
pobj |
orthologs,to |
R920 |
T1451 |
T1450 |
nmod |
yeast,orthologs |
R926 |
T1457 |
T1455 |
punct |
),S1 |
R927 |
T1458 |
T1459 |
punct |
[,12 |
R928 |
T1459 |
T1424 |
parataxis |
12,encodes |
R929 |
T1460 |
T1459 |
punct |
],12 |
R930 |
T1461 |
T1424 |
punct |
),encodes |
R931 |
T1462 |
T1424 |
punct |
.,encodes |
R932 |
T1464 |
T1465 |
advmod |
Interestingly,shows |
R933 |
T1466 |
T1465 |
punct |
", ",shows |
R934 |
T1467 |
T1468 |
amod |
phylogenetic,analysis |
R935 |
T1468 |
T1465 |
nsubj |
analysis,shows |
R936 |
T1469 |
T1468 |
prep |
of,analysis |
R937 |
T1470 |
T1471 |
compound |
TRIP13,Pch2p |
R938 |
T1471 |
T1469 |
pobj |
Pch2p,of |
R939 |
T1472 |
T1471 |
punct |
/,Pch2p |
R940 |
T1473 |
T1474 |
mark |
that,clusters |
R941 |
T1474 |
T1465 |
ccomp |
clusters,shows |
R942 |
T1475 |
T1476 |
det |
the,protein |
R943 |
T1476 |
T1474 |
nsubj |
protein,clusters |
R944 |
T1477 |
T1476 |
amod |
mammalian,protein |
R945 |
T1478 |
T1479 |
advmod |
more,closely |
R946 |
T1479 |
T1474 |
advmod |
closely,clusters |
R947 |
T1480 |
T1474 |
prep |
to,clusters |
R948 |
T1481 |
T1480 |
pobj |
plants,to |
R949 |
T1482 |
T1483 |
mark |
than,to |
R950 |
T1483 |
T1474 |
advcl |
to,clusters |
R951 |
T1484 |
T1483 |
nsubj |
it,to |
R952 |
T1485 |
T1483 |
aux |
does,to |
R953 |
T1486 |
T1487 |
det |
the,worms |
R954 |
T1487 |
T1483 |
pobj |
worms,to |
R955 |
T1488 |
T1489 |
advmod |
evolutionarily,related |
R956 |
T1489 |
T1487 |
amod |
related,worms |
R957 |
T1490 |
T1491 |
advmod |
more,closely |
R958 |
T1491 |
T1489 |
advmod |
closely,related |
R959 |
T1492 |
T1487 |
cc |
and,worms |
R960 |
T1493 |
T1487 |
conj |
flies,worms |
R961 |
T1494 |
T1495 |
punct |
(,1A |
R962 |
T1495 |
T1465 |
parataxis |
1A,shows |
R963 |
T1496 |
T1495 |
compound |
Figure,1A |
R964 |
T1497 |
T1495 |
punct |
;,1A |
R965 |
T1498 |
T1495 |
advcl |
see,1A |
R966 |
T1499 |
T1498 |
dobj |
Discussion,see |
R967 |
T1500 |
T1495 |
punct |
),1A |
R968 |
T1501 |
T1465 |
punct |
.,shows |
R969 |
T1503 |
T1504 |
amod |
Semi-quantitative,analysis |
R970 |
T1504 |
T1514 |
nsubj |
analysis,showed |
R971 |
T1505 |
T1506 |
nmod |
reverse,transcriptase |
R972 |
T1506 |
T1508 |
nmod |
transcriptase,PCR |
R973 |
T1507 |
T1506 |
punct |
-,transcriptase |
R974 |
T1508 |
T1504 |
nmod |
PCR,analysis |
R975 |
T1509 |
T1508 |
punct |
(,PCR |
R976 |
T1510 |
T1511 |
compound |
RT,PCR |
R977 |
T1511 |
T1508 |
appos |
PCR,PCR |
R978 |
T1512 |
T1511 |
punct |
-,PCR |
R979 |
T1513 |
T1504 |
punct |
),analysis |
R980 |
T1515 |
T1516 |
compound |
Trip13,mRNA |
R981 |
T1516 |
T1517 |
nsubjpass |
mRNA,expressed |
R982 |
T1517 |
T1514 |
ccomp |
expressed,showed |
R983 |
T1518 |
T1517 |
aux |
to,expressed |
R984 |
T1519 |
T1517 |
auxpass |
be,expressed |
R985 |
T1520 |
T1517 |
prep |
in,expressed |
R986 |
T1521 |
T1522 |
det |
a,variety |
R987 |
T1522 |
T1520 |
pobj |
variety,in |
R988 |
T1523 |
T1522 |
prep |
of,variety |
R989 |
T1524 |
T1525 |
amod |
embryonic,tissues |
R990 |
T1525 |
T1523 |
pobj |
tissues,of |
R991 |
T1526 |
T1524 |
cc |
and,embryonic |
R992 |
T1527 |
T1524 |
conj |
adult,embryonic |
R993 |
T1528 |
T1525 |
punct |
", ",tissues |
R994 |
T1529 |
T1525 |
prep |
including,tissues |
R995 |
T1530 |
T1529 |
pobj |
testis,including |
R996 |
T1531 |
T1532 |
punct |
(,1B |
R997 |
T1532 |
T1530 |
parataxis |
1B,testis |
R998 |
T1533 |
T1532 |
compound |
Figure,1B |
R999 |
T1534 |
T1532 |
punct |
),1B |
R1000 |
T1535 |
T1517 |
punct |
", ",expressed |
R1001 |
T1536 |
T1517 |
advcl |
consistent,expressed |
R1002 |
T1537 |
T1536 |
prep |
with,consistent |
R1003 |
T1538 |
T1539 |
nmod |
mouse,EST |
R1004 |
T1539 |
T1542 |
compound |
EST,data |
R1005 |
T1540 |
T1538 |
cc |
and,mouse |
R1006 |
T1541 |
T1538 |
conj |
human,mouse |
R1007 |
T1542 |
T1537 |
pobj |
data,with |
R1008 |
T1543 |
T1542 |
acl |
summarized,data |
R1009 |
T1544 |
T1543 |
prep |
in,summarized |
R1010 |
T1545 |
T1544 |
pobj |
Unigene,in |
R1011 |
T1546 |
T1547 |
punct |
(,http://www.ncbi.nlm.nih.gov/UniGene |
R1012 |
T1547 |
T1545 |
parataxis |
http://www.ncbi.nlm.nih.gov/UniGene,Unigene |
R1013 |
T1548 |
T1547 |
punct |
),http://www.ncbi.nlm.nih.gov/UniGene |
R1014 |
T1549 |
T1514 |
punct |
.,showed |
R1015 |
T1551 |
T1552 |
nsubjpass |
It,expressed |
R1016 |
T1553 |
T1552 |
auxpass |
is,expressed |
R1017 |
T1554 |
T1552 |
advmod |
also,expressed |
R1018 |
T1555 |
T1552 |
advmod |
highly,expressed |
R1019 |
T1556 |
T1552 |
prep |
in,expressed |
R1020 |
T1557 |
T1558 |
amod |
human,mouse |
R1021 |
T1558 |
T1560 |
compound |
mouse,oocytes |
R1022 |
T1559 |
T1558 |
cc |
and,mouse |
R1023 |
T1560 |
T1556 |
pobj |
oocytes,in |
R1024 |
T1561 |
T1562 |
punct |
[,17 |
R1025 |
T1562 |
T1552 |
parataxis |
17,expressed |
R1026 |
T1563 |
T1562 |
punct |
],17 |
R1027 |
T1564 |
T1552 |
punct |
.,expressed |
R6842 |
T10169 |
T10170 |
det |
The,Ortholog |
R6843 |
T10171 |
T10172 |
compound |
Mouse,PCH2 |
R6844 |
T10172 |
T10170 |
compound |
PCH2,Ortholog |
R6845 |
T10173 |
T10170 |
appos |
TRIP13,Ortholog |
R6846 |
T10174 |
T10170 |
cc |
and,Ortholog |
R6847 |
T10175 |
T10170 |
conj |
Expression,Ortholog |
R6848 |
T10176 |
T10175 |
prep |
in,Expression |
R6849 |
T10177 |
T10178 |
amod |
Wild,Type |
R6850 |
T10178 |
T10176 |
pobj |
Type,in |
R6851 |
T10179 |
T10178 |
cc |
and,Type |
R6852 |
T10180 |
T10178 |
conj |
Mutant,Type |
R6853 |
T10182 |
T10183 |
punct |
(,A |
R6854 |
T10183 |
T10184 |
meta |
A,tree |
R6855 |
T10185 |
T10183 |
punct |
),A |
R6856 |
T10186 |
T10184 |
amod |
Phylogenetic,tree |
R6857 |
T10187 |
T10184 |
prep |
of,tree |
R6858 |
T10188 |
T10189 |
amod |
presumed,orthologs |
R6859 |
T10189 |
T10187 |
pobj |
orthologs,of |
R6860 |
T10190 |
T10191 |
compound |
PCH2,TRIP13 |
R6861 |
T10191 |
T10189 |
compound |
TRIP13,orthologs |
R6862 |
T10192 |
T10191 |
punct |
/,TRIP13 |
R6863 |
T10193 |
T10184 |
punct |
.,tree |
R6864 |
T10195 |
T10196 |
det |
The,number |
R6865 |
T10196 |
T10200 |
nsubjpass |
number,presented |
R6866 |
T10197 |
T10198 |
compound |
database,sequence |
R6867 |
T10198 |
T10199 |
compound |
sequence,accession |
R6868 |
T10199 |
T10196 |
compound |
accession,number |
R6869 |
T10201 |
T10196 |
prep |
of,number |
R6870 |
T10202 |
T10203 |
det |
each,protein |
R6871 |
T10203 |
T10201 |
pobj |
protein,of |
R6872 |
T10204 |
T10200 |
auxpass |
is,presented |
R6873 |
T10205 |
T10200 |
prep |
in,presented |
R6874 |
T10206 |
T10207 |
compound |
Table,S1 |
R6875 |
T10207 |
T10205 |
pobj |
S1,in |
R6876 |
T10208 |
T10200 |
punct |
.,presented |
R6877 |
T10210 |
T10211 |
nsubj |
Numbers,are |
R6878 |
T10212 |
T10210 |
acl |
shown,Numbers |
R6879 |
T10213 |
T10214 |
compound |
bootstrap,values |
R6880 |
T10214 |
T10211 |
attr |
values,are |
R6881 |
T10215 |
T10216 |
punct |
(,see |
R6882 |
T10216 |
T10211 |
parataxis |
see,are |
R6883 |
T10217 |
T10216 |
dobj |
Materials,see |
R6884 |
T10218 |
T10217 |
cc |
and,Materials |
R6885 |
T10219 |
T10217 |
conj |
Methods,Materials |
R6886 |
T10220 |
T10216 |
punct |
),see |
R6887 |
T10221 |
T10211 |
punct |
.,are |
R6888 |
T10223 |
T10224 |
det |
The,clustering |
R6889 |
T10224 |
T10225 |
nsubj |
clustering,was |
R6890 |
T10226 |
T10227 |
det |
the,same |
R6891 |
T10227 |
T10225 |
attr |
same,was |
R6892 |
T10228 |
T10225 |
advmod |
regardless,was |
R6893 |
T10229 |
T10228 |
prep |
of,regardless |
R6894 |
T10230 |
T10231 |
mark |
whether,used |
R6895 |
T10231 |
T10229 |
pcomp |
used,of |
R6896 |
T10232 |
T10233 |
det |
the,sequence |
R6897 |
T10233 |
T10231 |
nsubjpass |
sequence,used |
R6898 |
T10234 |
T10235 |
amod |
whole,entire |
R6899 |
T10235 |
T10233 |
amod |
entire,sequence |
R6900 |
T10236 |
T10233 |
compound |
AA,sequence |
R6901 |
T10237 |
T10233 |
cc |
or,sequence |
R6902 |
T10238 |
T10239 |
amod |
trimmed,sequences |
R6903 |
T10239 |
T10233 |
conj |
sequences,sequence |
R6904 |
T10240 |
T10239 |
punct |
(,sequences |
R6905 |
T10241 |
T10242 |
advmod |
where,removed |
R6906 |
T10242 |
T10239 |
relcl |
removed,sequences |
R6907 |
T10243 |
T10242 |
nsubjpass |
regions,removed |
R6908 |
T10244 |
T10243 |
acl |
showing,regions |
R6909 |
T10245 |
T10246 |
amod |
little,conservation |
R6910 |
T10246 |
T10244 |
dobj |
conservation,showing |
R6911 |
T10247 |
T10242 |
auxpass |
were,removed |
R6912 |
T10248 |
T10231 |
punct |
),used |
R6913 |
T10249 |
T10231 |
auxpass |
were,used |
R6914 |
T10250 |
T10231 |
prep |
for,used |
R6915 |
T10251 |
T10252 |
det |
the,analysis |
R6916 |
T10252 |
T10250 |
pobj |
analysis,for |
R6917 |
T10253 |
T10225 |
punct |
.,was |
R6918 |
T10255 |
T10256 |
amod |
Major,groups |
R6919 |
T10256 |
T10258 |
nsubjpass |
groups,indicated |
R6920 |
T10257 |
T10256 |
amod |
eukaryotic,groups |
R6921 |
T10259 |
T10258 |
auxpass |
are,indicated |
R6922 |
T10260 |
T10258 |
prep |
in,indicated |
R6923 |
T10261 |
T10260 |
pobj |
color,in |
R6924 |
T10262 |
T10258 |
punct |
", ",indicated |
R6925 |
T10263 |
T10264 |
mark |
with,in |
R6926 |
T10264 |
T10258 |
advcl |
in,indicated |
R6927 |
T10265 |
T10264 |
nsubj |
deuterostomia,in |
R6928 |
T10266 |
T10264 |
pobj |
blue,in |
R6929 |
T10267 |
T10264 |
punct |
", ",in |
R6930 |
T10268 |
T10269 |
nsubj |
plants,in |
R6931 |
T10269 |
T10264 |
conj |
in,in |
R6932 |
T10270 |
T10269 |
pobj |
green,in |
R6933 |
T10271 |
T10269 |
punct |
", ",in |
R6934 |
T10272 |
T10273 |
nsubj |
protostomia,in |
R6935 |
T10273 |
T10269 |
conj |
in,in |
R6936 |
T10274 |
T10273 |
pobj |
purple,in |
R6937 |
T10275 |
T10273 |
punct |
", ",in |
R6938 |
T10276 |
T10273 |
cc |
and,in |
R6939 |
T10277 |
T10278 |
nsubj |
fungi,in |
R6940 |
T10278 |
T10273 |
conj |
in,in |
R6941 |
T10279 |
T10278 |
pobj |
maroon,in |
R6942 |
T10280 |
T10258 |
punct |
.,indicated |
R6943 |
T10282 |
T10283 |
punct |
(,B |
R6944 |
T10283 |
T10284 |
meta |
B,products |
R6945 |
T10285 |
T10283 |
punct |
),B |
R6946 |
T10286 |
T10284 |
compound |
Amplification,products |
R6947 |
T10287 |
T10284 |
prep |
of,products |
R6948 |
T10288 |
T10287 |
pobj |
cDNA,of |
R6949 |
T10289 |
T10284 |
prep |
from,products |
R6950 |
T10290 |
T10291 |
det |
the,tissues |
R6951 |
T10291 |
T10289 |
pobj |
tissues,from |
R6952 |
T10292 |
T10291 |
amod |
following,tissues |
R6953 |
T10293 |
T10291 |
punct |
: ,tissues |
R6954 |
T10294 |
T10295 |
meta |
1,heart |
R6955 |
T10295 |
T10291 |
appos |
heart,tissues |
R6956 |
T10296 |
T10295 |
punct |
", ",heart |
R6957 |
T10297 |
T10295 |
punct |
;,heart |
R6958 |
T10298 |
T10299 |
meta |
2,brain |
R6959 |
T10299 |
T10295 |
conj |
brain,heart |
R6960 |
T10300 |
T10299 |
punct |
", ",brain |
R6961 |
T10301 |
T10299 |
punct |
;,brain |
R6962 |
T10302 |
T10303 |
meta |
3,spleen |
R6963 |
T10303 |
T10299 |
conj |
spleen,brain |
R6964 |
T10304 |
T10303 |
punct |
", ",spleen |
R6965 |
T10305 |
T10303 |
punct |
;,spleen |
R6966 |
T10306 |
T10307 |
meta |
4,lung |
R6967 |
T10307 |
T10303 |
conj |
lung,spleen |
R6968 |
T10308 |
T10307 |
punct |
", ",lung |
R6969 |
T10309 |
T10307 |
punct |
;,lung |
R6970 |
T10310 |
T10311 |
meta |
5,liver |
R6971 |
T10311 |
T10307 |
conj |
liver,lung |
R6972 |
T10312 |
T10311 |
punct |
", ",liver |
R6973 |
T10313 |
T10311 |
punct |
;,liver |
R6974 |
T10314 |
T10315 |
meta |
6,muscle |
R6975 |
T10315 |
T10311 |
conj |
muscle,liver |
R6976 |
T10316 |
T10315 |
punct |
", ",muscle |
R6977 |
T10317 |
T10315 |
amod |
skeletal,muscle |
R6978 |
T10318 |
T10315 |
punct |
;,muscle |
R6979 |
T10319 |
T10320 |
meta |
7,kidney |
R6980 |
T10320 |
T10315 |
conj |
kidney,muscle |
R6981 |
T10321 |
T10320 |
punct |
", ",kidney |
R6982 |
T10322 |
T10320 |
punct |
;,kidney |
R6983 |
T10323 |
T10324 |
meta |
8,testis |
R6984 |
T10324 |
T10320 |
conj |
testis,kidney |
R6985 |
T10325 |
T10324 |
punct |
", ",testis |
R6986 |
T10326 |
T10324 |
punct |
;,testis |
R6987 |
T10327 |
T10328 |
meta |
9,embryo |
R6988 |
T10328 |
T10324 |
conj |
embryo,testis |
R6989 |
T10329 |
T10328 |
punct |
", ",embryo |
R6990 |
T10330 |
T10328 |
compound |
E7,embryo |
R6991 |
T10331 |
T10328 |
punct |
;,embryo |
R6992 |
T10332 |
T10333 |
meta |
10,embryo |
R6993 |
T10333 |
T10328 |
conj |
embryo,embryo |
R6994 |
T10334 |
T10333 |
punct |
", ",embryo |
R6995 |
T10335 |
T10333 |
compound |
E11,embryo |
R6996 |
T10336 |
T10333 |
punct |
;,embryo |
R6997 |
T10337 |
T10338 |
meta |
11,embryo |
R6998 |
T10338 |
T10333 |
conj |
embryo,embryo |
R6999 |
T10339 |
T10338 |
punct |
", ",embryo |
R7000 |
T10340 |
T10338 |
compound |
E15,embryo |
R7001 |
T10341 |
T10338 |
punct |
;,embryo |
R7002 |
T10342 |
T10338 |
cc |
and,embryo |
R7003 |
T10343 |
T10344 |
meta |
12,embryo |
R7004 |
T10344 |
T10338 |
conj |
embryo,embryo |
R7005 |
T10345 |
T10344 |
punct |
", ",embryo |
R7006 |
T10346 |
T10344 |
compound |
E17,embryo |
R7007 |
T10347 |
T10284 |
punct |
.,products |
R7008 |
T10349 |
T10350 |
punct |
(,C |
R7009 |
T10350 |
T10351 |
meta |
C,structure |
R7010 |
T10352 |
T10350 |
punct |
),C |
R7011 |
T10353 |
T10354 |
compound |
Intron,exon |
R7012 |
T10354 |
T10351 |
compound |
exon,structure |
R7013 |
T10355 |
T10354 |
punct |
–,exon |
R7014 |
T10356 |
T10351 |
prep |
of,structure |
R7015 |
T10357 |
T10356 |
pobj |
TRIP13,of |
R7016 |
T10358 |
T10351 |
cc |
and,structure |
R7017 |
T10359 |
T10360 |
compound |
insertion,site |
R7018 |
T10360 |
T10351 |
conj |
site,structure |
R7019 |
T10361 |
T10360 |
prep |
of,site |
R7020 |
T10362 |
T10363 |
compound |
gene,trap |
R7021 |
T10363 |
T10365 |
compound |
trap,vector |
R7022 |
T10364 |
T10363 |
punct |
-,trap |
R7023 |
T10365 |
T10361 |
pobj |
vector,of |
R7024 |
T10366 |
T10360 |
punct |
.,site |
R7025 |
T10369 |
T10368 |
dobj |
Materials,See |
R7026 |
T10370 |
T10369 |
cc |
and,Materials |
R7027 |
T10371 |
T10369 |
conj |
Methods,Materials |
R7028 |
T10372 |
T10368 |
prep |
for,See |
R7029 |
T10373 |
T10372 |
pobj |
details,for |
R7030 |
T10374 |
T10373 |
prep |
on,details |
R7031 |
T10375 |
T10376 |
advmod |
how,identified |
R7032 |
T10376 |
T10374 |
pcomp |
identified,on |
R7033 |
T10377 |
T10378 |
det |
the,site |
R7034 |
T10378 |
T10376 |
nsubjpass |
site,identified |
R7035 |
T10379 |
T10378 |
amod |
precise,site |
R7036 |
T10380 |
T10378 |
compound |
insertion,site |
R7037 |
T10381 |
T10376 |
auxpass |
was,identified |
R7038 |
T10382 |
T10368 |
punct |
.,See |
R7039 |
T10384 |
T10385 |
punct |
(,D |
R7040 |
T10385 |
T10386 |
meta |
D,PCR |
R7041 |
T10387 |
T10385 |
punct |
),D |
R7042 |
T10388 |
T10386 |
compound |
RT,PCR |
R7043 |
T10389 |
T10386 |
punct |
-,PCR |
R7044 |
T10390 |
T10386 |
prep |
of,PCR |
R7045 |
T10391 |
T10390 |
pobj |
Trip13,of |
R7046 |
T10392 |
T10391 |
cc |
and,Trip13 |
R7047 |
T10393 |
T10394 |
det |
a,Med31 |
R7048 |
T10394 |
T10391 |
conj |
Med31,Trip13 |
R7049 |
T10395 |
T10394 |
compound |
control,Med31 |
R7050 |
T10396 |
T10394 |
compound |
gene,Med31 |
R7051 |
T10397 |
T10394 |
prep |
from,Med31 |
R7052 |
T10398 |
T10399 |
compound |
testis,RNA |
R7053 |
T10399 |
T10397 |
pobj |
RNA,from |
R7054 |
T10400 |
T10386 |
punct |
.,PCR |
R7055 |
T10402 |
T10403 |
det |
The,primers |
R7056 |
T10403 |
T10405 |
nsubjpass |
primers,situated |
R7057 |
T10404 |
T10403 |
compound |
Trip13,primers |
R7058 |
T10406 |
T10405 |
auxpass |
are,situated |
R7059 |
T10407 |
T10405 |
prep |
in,situated |
R7060 |
T10408 |
T10409 |
det |
the,exons |
R7061 |
T10409 |
T10407 |
pobj |
exons,in |
R7062 |
T10410 |
T10409 |
amod |
first,exons |
R7063 |
T10411 |
T10410 |
cc |
and,first |
R7064 |
T10412 |
T10410 |
conj |
last,first |
R7065 |
T10413 |
T10414 |
punct |
(,see |
R7066 |
T10414 |
T10405 |
parataxis |
see,situated |
R7067 |
T10415 |
T10414 |
dobj |
Materials,see |
R7068 |
T10416 |
T10415 |
cc |
and,Materials |
R7069 |
T10417 |
T10415 |
conj |
Methods,Materials |
R7070 |
T10418 |
T10414 |
punct |
),see |
R7071 |
T10419 |
T10405 |
punct |
.,situated |
R7072 |
T10421 |
T10422 |
punct |
(,E |
R7073 |
T10422 |
T10423 |
meta |
E,analysis |
R7074 |
T10424 |
T10422 |
punct |
),E |
R7075 |
T10425 |
T10426 |
compound |
Western,blot |
R7076 |
T10426 |
T10423 |
compound |
blot,analysis |
R7077 |
T10427 |
T10423 |
prep |
of,analysis |
R7078 |
T10428 |
T10429 |
compound |
testis,protein |
R7079 |
T10429 |
T10427 |
pobj |
protein,of |
R7080 |
T10430 |
T10423 |
prep |
with,analysis |
R7081 |
T10431 |
T10432 |
amod |
anti-TRIP13,antibody |
R7082 |
T10432 |
T10430 |
pobj |
antibody,with |
R7083 |
T10433 |
T10423 |
punct |
.,analysis |
R7084 |
T10435 |
T10436 |
det |
The,blot |
R7085 |
T10436 |
T10437 |
nsubjpass |
blot,probed |
R7086 |
T10438 |
T10437 |
auxpass |
was,probed |
R7087 |
T10439 |
T10437 |
advmod |
later,probed |
R7088 |
T10440 |
T10437 |
prep |
with,probed |
R7089 |
T10441 |
T10442 |
amod |
anti-alpha,actin |
R7090 |
T10442 |
T10440 |
pobj |
actin,with |
R7091 |
T10443 |
T10442 |
compound |
tubulin,actin |
R7092 |
T10444 |
T10442 |
prep |
as,actin |
R7093 |
T10445 |
T10446 |
det |
a,control |
R7094 |
T10446 |
T10444 |
pobj |
control,as |
R7095 |
T10447 |
T10446 |
compound |
loading,control |
R7096 |
T10448 |
T10437 |
punct |
.,probed |
R7097 |
T10450 |
T10451 |
det |
The,protein |
R7098 |
T10451 |
T10454 |
nsubj |
protein,is |
R7099 |
T10452 |
T10451 |
amod |
expected,protein |
R7100 |
T10453 |
T10451 |
compound |
TRIP13,protein |
R7101 |
T10455 |
T10456 |
punct |
∼,48 |
R7102 |
T10456 |
T10457 |
nummod |
48,KDa |
R7103 |
T10457 |
T10454 |
attr |
KDa,is |
R7104 |
T10458 |
T10454 |
punct |
.,is |
R7105 |
T10460 |
T10461 |
punct |
(,F |
R7106 |
T10461 |
T10462 |
meta |
F,Localization |
R7107 |
T10463 |
T10461 |
punct |
),F |
R7108 |
T10464 |
T10462 |
prep |
of,Localization |
R7109 |
T10465 |
T10464 |
pobj |
TRIP13,of |
R7110 |
T10466 |
T10462 |
prep |
in,Localization |
R7111 |
T10467 |
T10466 |
pobj |
testes,in |
R7112 |
T10468 |
T10462 |
punct |
.,Localization |
R7113 |
T10470 |
T10471 |
amod |
Wild,type |
R7114 |
T10471 |
T10473 |
nmod |
type,testis |
R7115 |
T10472 |
T10471 |
punct |
-,type |
R7116 |
T10473 |
T10482 |
compound |
testis,sections |
R7117 |
T10474 |
T10475 |
punct |
(,top |
R7118 |
T10475 |
T10471 |
parataxis |
top,type |
R7119 |
T10476 |
T10475 |
punct |
),top |
R7120 |
T10477 |
T10471 |
cc |
and,type |
R7121 |
T10478 |
T10471 |
conj |
mutant,type |
R7122 |
T10479 |
T10480 |
punct |
(,bottom |
R7123 |
T10480 |
T10478 |
parataxis |
bottom,mutant |
R7124 |
T10481 |
T10480 |
punct |
),bottom |
R7125 |
T10482 |
T10483 |
nsubjpass |
sections,probed |
R7126 |
T10484 |
T10483 |
auxpass |
were,probed |
R7127 |
T10485 |
T10483 |
prep |
with,probed |
R7128 |
T10486 |
T10485 |
pobj |
chicken,with |
R7129 |
T10487 |
T10486 |
amod |
anti-TRIP13,chicken |
R7130 |
T10488 |
T10483 |
punct |
", ",probed |
R7131 |
T10489 |
T10483 |
cc |
and,probed |
R7132 |
T10490 |
T10483 |
conj |
detected,probed |
R7133 |
T10491 |
T10490 |
prep |
with,detected |
R7134 |
T10492 |
T10493 |
npadvmod |
HRP,conjugated |
R7135 |
T10493 |
T10495 |
amod |
conjugated,IgG |
R7136 |
T10494 |
T10493 |
punct |
-,conjugated |
R7137 |
T10495 |
T10491 |
pobj |
IgG,with |
R7138 |
T10496 |
T10495 |
amod |
anti-chicken,IgG |
R7139 |
T10497 |
T10498 |
punct |
(,staining |
R7140 |
T10498 |
T10495 |
parataxis |
staining,IgG |
R7141 |
T10499 |
T10500 |
amod |
brown,red |
R7142 |
T10500 |
T10498 |
amod |
red,staining |
R7143 |
T10501 |
T10500 |
punct |
/,red |
R7144 |
T10502 |
T10498 |
punct |
),staining |
R7145 |
T10503 |
T10483 |
punct |
.,probed |
R7146 |
T10505 |
T10506 |
nsubj |
Expression,was |
R7147 |
T10507 |
T10505 |
prep |
in,Expression |
R7148 |
T10508 |
T10507 |
pobj |
WT,in |
R7149 |
T10509 |
T10510 |
advmod |
most,prominent |
R7150 |
T10510 |
T10506 |
acomp |
prominent,was |
R7151 |
T10511 |
T10506 |
prep |
in,was |
R7152 |
T10512 |
T10513 |
det |
the,nuclei |
R7153 |
T10513 |
T10511 |
pobj |
nuclei,in |
R7154 |
T10514 |
T10513 |
prep |
of,nuclei |
R7155 |
T10515 |
T10516 |
compound |
Type,B |
R7156 |
T10516 |
T10517 |
compound |
B,spermatogonia |
R7157 |
T10517 |
T10514 |
pobj |
spermatogonia,of |
R7158 |
T10518 |
T10517 |
punct |
(,spermatogonia |
R7159 |
T10519 |
T10517 |
appos |
Sg,spermatogonia |
R7160 |
T10520 |
T10517 |
punct |
),spermatogonia |
R7161 |
T10521 |
T10517 |
punct |
", ",spermatogonia |
R7162 |
T10522 |
T10523 |
compound |
leptotene,spermatocytes |
R7163 |
T10523 |
T10517 |
conj |
spermatocytes,spermatogonia |
R7164 |
T10524 |
T10523 |
punct |
(,spermatocytes |
R7165 |
T10525 |
T10523 |
appos |
LS,spermatocytes |
R7166 |
T10526 |
T10523 |
punct |
),spermatocytes |
R7167 |
T10527 |
T10523 |
punct |
", ",spermatocytes |
R7168 |
T10528 |
T10523 |
cc |
and,spermatocytes |
R7169 |
T10529 |
T10530 |
amod |
early,spermatocytes |
R7170 |
T10530 |
T10523 |
conj |
spermatocytes,spermatocytes |
R7171 |
T10531 |
T10530 |
compound |
pachytene,spermatocytes |
R7172 |
T10532 |
T10530 |
punct |
(,spermatocytes |
R7173 |
T10533 |
T10530 |
appos |
PS,spermatocytes |
R7174 |
T10534 |
T10530 |
punct |
),spermatocytes |
R7175 |
T10535 |
T10530 |
punct |
", ",spermatocytes |
R7176 |
T10536 |
T10530 |
cc |
but,spermatocytes |
R7177 |
T10537 |
T10536 |
neg |
not,but |
R7178 |
T10538 |
T10539 |
amod |
late,spermatocytes |
R7179 |
T10539 |
T10530 |
conj |
spermatocytes,spermatocytes |
R7180 |
T10540 |
T10539 |
compound |
pachytene,spermatocytes |
R7181 |
T10541 |
T10539 |
punct |
(,spermatocytes |
R7182 |
T10542 |
T10539 |
appos |
LP,spermatocytes |
R7183 |
T10543 |
T10506 |
punct |
),was |
R7184 |
T10544 |
T10506 |
punct |
.,was |
R7185 |
T10546 |
T10547 |
det |
No,staining |
R7186 |
T10547 |
T10549 |
nsubjpass |
staining,seen |
R7187 |
T10548 |
T10547 |
amod |
nuclear,staining |
R7188 |
T10550 |
T10549 |
auxpass |
was,seen |
R7189 |
T10551 |
T10549 |
prep |
in,seen |
R7190 |
T10552 |
T10553 |
compound |
mutant,sections |
R7191 |
T10553 |
T10551 |
pobj |
sections,in |
R7192 |
T10554 |
T10553 |
compound |
testis,sections |
R7193 |
T10555 |
T10549 |
punct |
", ",seen |
R7194 |
T10556 |
T10557 |
mark |
although,is |
R7195 |
T10557 |
T10549 |
advcl |
is,seen |
R7196 |
T10558 |
T10559 |
amod |
reddish,background |
R7197 |
T10559 |
T10557 |
nsubj |
background,is |
R7198 |
T10560 |
T10559 |
amod |
cytoplasmic,background |
R7199 |
T10561 |
T10557 |
acomp |
present,is |
R7200 |
T10562 |
T10549 |
punct |
.,seen |
R7201 |
T10564 |
T10565 |
nsubjpass |
Identification,judged |
R7202 |
T10566 |
T10564 |
prep |
of,Identification |
R7203 |
T10567 |
T10568 |
compound |
cell,types |
R7204 |
T10568 |
T10566 |
pobj |
types,of |
R7205 |
T10569 |
T10565 |
auxpass |
was,judged |
R7206 |
T10570 |
T10565 |
prep |
in,judged |
R7207 |
T10571 |
T10570 |
amod |
part,in |
R7208 |
T10572 |
T10565 |
prep |
by,judged |
R7209 |
T10573 |
T10572 |
pcomp |
estimating,by |
R7210 |
T10574 |
T10575 |
det |
the,stage |
R7211 |
T10575 |
T10573 |
dobj |
stage,estimating |
R7212 |
T10576 |
T10575 |
amod |
epithelial,stage |
R7213 |
T10577 |
T10575 |
prep |
of,stage |
R7214 |
T10578 |
T10579 |
det |
the,tubules |
R7215 |
T10579 |
T10577 |
pobj |
tubules,of |
R7216 |
T10580 |
T10581 |
mark |
as,described |
R7217 |
T10581 |
T10573 |
advcl |
described,estimating |
R7218 |
T10582 |
T10583 |
punct |
[,67 |
R7219 |
T10583 |
T10573 |
parataxis |
67,estimating |
R7220 |
T10584 |
T10583 |
punct |
],67 |
R7221 |
T10585 |
T10565 |
punct |
.,judged |
R7222 |
T10587 |
T10588 |
punct |
(,G |
R7223 |
T10588 |
T10589 |
meta |
G,localization |
R7224 |
T10590 |
T10588 |
punct |
),G |
R7225 |
T10591 |
T10589 |
compound |
TRIP13,localization |
R7226 |
T10592 |
T10589 |
prep |
in,localization |
R7227 |
T10593 |
T10594 |
compound |
surface,spread |
R7228 |
T10594 |
T10596 |
compound |
spread,spermatocytes |
R7229 |
T10595 |
T10594 |
punct |
-,spread |
R7230 |
T10596 |
T10592 |
pobj |
spermatocytes,in |
R7231 |
T10597 |
T10589 |
punct |
.,localization |
R7232 |
T10599 |
T10600 |
nsubjpass |
Preparations,immunolabeled |
R7233 |
T10601 |
T10600 |
auxpass |
were,immunolabeled |
R7234 |
T10602 |
T10600 |
prep |
with,immunolabeled |
R7235 |
T10603 |
T10602 |
pobj |
anti-SYCP3,with |
R7236 |
T10604 |
T10603 |
punct |
(,anti-SYCP3 |
R7237 |
T10605 |
T10603 |
appos |
S,anti-SYCP3 |
R7238 |
T10606 |
T10603 |
punct |
),anti-SYCP3 |
R7239 |
T10607 |
T10603 |
cc |
and,anti-SYCP3 |
R7240 |
T10608 |
T10603 |
conj |
TRIP13,anti-SYCP3 |
R7241 |
T10609 |
T10608 |
punct |
(,TRIP13 |
R7242 |
T10610 |
T10608 |
appos |
T,TRIP13 |
R7243 |
T10611 |
T10600 |
punct |
),immunolabeled |
R7244 |
T10612 |
T10600 |
punct |
.,immunolabeled |
R7245 |
T10614 |
T10615 |
preconj |
Both,individual |
R7246 |
T10615 |
T10616 |
amod |
individual,images |
R7247 |
T10616 |
T10619 |
nsubjpass |
images,shown |
R7248 |
T10617 |
T10615 |
cc |
and,individual |
R7249 |
T10618 |
T10615 |
conj |
merged,individual |
R7250 |
T10620 |
T10619 |
auxpass |
are,shown |
R7251 |
T10621 |
T10619 |
prep |
for,shown |
R7252 |
T10622 |
T10623 |
nmod |
leptotene,spermatocytes |
R7253 |
T10623 |
T10621 |
pobj |
spermatocytes,for |
R7254 |
T10624 |
T10622 |
punct |
(,leptotene |
R7255 |
T10625 |
T10622 |
appos |
Lep,leptotene |
R7256 |
T10626 |
T10622 |
punct |
),leptotene |
R7257 |
T10627 |
T10622 |
punct |
", ",leptotene |
R7258 |
T10628 |
T10622 |
conj |
zygotene,leptotene |
R7259 |
T10629 |
T10628 |
punct |
(,zygotene |
R7260 |
T10630 |
T10628 |
appos |
Zyg,zygotene |
R7261 |
T10631 |
T10628 |
punct |
),zygotene |
R7262 |
T10632 |
T10628 |
punct |
", ",zygotene |
R7263 |
T10633 |
T10628 |
cc |
and,zygotene |
R7264 |
T10634 |
T10628 |
conj |
pachytene,zygotene |
R7265 |
T10635 |
T10634 |
punct |
(,pachytene |
R7266 |
T10636 |
T10634 |
appos |
Pac,pachytene |
R7267 |
T10637 |
T10623 |
punct |
),spermatocytes |
R7268 |
T10638 |
T10619 |
punct |
.,shown |
R7269 |
T10640 |
T10641 |
amod |
Nuclear,staining |
R7270 |
T10641 |
T10642 |
nsubj |
staining,was |
R7271 |
T10643 |
T10642 |
acomp |
absent,was |
R7272 |
T10644 |
T10642 |
prep |
in,was |
R7273 |
T10645 |
T10646 |
det |
the,mutant |
R7274 |
T10646 |
T10644 |
pobj |
mutant,in |
R7275 |
T10647 |
T10642 |
punct |
.,was |