Id |
Subject |
Object |
Predicate |
Lexical cue |
T9028 |
0-2 |
CD |
denotes |
50 |
T9029 |
3-5 |
NN |
denotes |
μg |
T9030 |
43-52 |
VBN |
denotes |
separated |
T9031 |
6-8 |
IN |
denotes |
of |
T9032 |
9-15 |
NN |
denotes |
testis |
T9033 |
16-23 |
NN |
denotes |
extract |
T9034 |
24-26 |
IN |
denotes |
in |
T9035 |
27-31 |
NN |
denotes |
RIPA |
T9036 |
32-38 |
NN |
denotes |
buffer |
T9037 |
39-42 |
VBD |
denotes |
was |
T9038 |
53-55 |
IN |
denotes |
by |
T9039 |
56-57 |
CD |
denotes |
8 |
T9040 |
57-58 |
NN |
denotes |
% |
T9041 |
63-67 |
NN |
denotes |
PAGE |
T9042 |
59-62 |
NN |
denotes |
SDS |
T9043 |
62-63 |
HYPH |
denotes |
- |
T9044 |
68-71 |
CC |
denotes |
and |
T9045 |
72-90 |
VBD |
denotes |
electrotransferred |
T9046 |
91-95 |
IN |
denotes |
onto |
T9047 |
96-97 |
DT |
denotes |
a |
T9048 |
118-126 |
NN |
denotes |
membrane |
T9049 |
98-102 |
NNP |
denotes |
Pure |
T9050 |
103-117 |
NNP |
denotes |
Nitrocellulose |
T9051 |
127-128 |
-LRB- |
denotes |
( |
T9052 |
132-135 |
NNP |
denotes |
Rad |
T9053 |
128-131 |
NNP |
denotes |
Bio |
T9054 |
131-132 |
HYPH |
denotes |
- |
T9055 |
135-137 |
, |
denotes |
, |
T9056 |
137-158 |
NN |
denotes |
http://www.biorad.com |
T9057 |
158-159 |
-RRB- |
denotes |
) |
T9058 |
159-160 |
. |
denotes |
. |
T9059 |
160-289 |
sentence |
denotes |
The membrane was incubated with a polyclonal rabbit anti-human TRIP13 antibody (18-003-42687; Genway, http://www.genwaybio.com). |
T9060 |
161-164 |
DT |
denotes |
The |
T9061 |
165-173 |
NN |
denotes |
membrane |
T9062 |
178-187 |
VBN |
denotes |
incubated |
T9063 |
174-177 |
VBD |
denotes |
was |
T9064 |
188-192 |
IN |
denotes |
with |
T9065 |
193-194 |
DT |
denotes |
a |
T9066 |
231-239 |
NN |
denotes |
antibody |
T9067 |
195-205 |
JJ |
denotes |
polyclonal |
T9068 |
206-212 |
NN |
denotes |
rabbit |
T9069 |
213-223 |
JJ |
denotes |
anti-human |
T9070 |
224-230 |
NN |
denotes |
TRIP13 |
T9071 |
240-241 |
-LRB- |
denotes |
( |
T9072 |
255-261 |
NNP |
denotes |
Genway |
T9073 |
241-243 |
CD |
denotes |
18 |
T9074 |
248-253 |
CD |
denotes |
42687 |
T9075 |
243-244 |
HYPH |
denotes |
- |
T9076 |
244-247 |
CD |
denotes |
003 |
T9077 |
247-248 |
HYPH |
denotes |
- |
T9078 |
253-254 |
: |
denotes |
; |
T9079 |
261-263 |
, |
denotes |
, |
T9080 |
263-287 |
NN |
denotes |
http://www.genwaybio.com |
T9081 |
287-288 |
-RRB- |
denotes |
) |
T9082 |
288-289 |
. |
denotes |
. |
T9083 |
289-415 |
sentence |
denotes |
According to the manufacturer, the immunogen was a synthetic peptide embedded in sequence we deduced to correspond to exon 3. |
T9084 |
290-299 |
VBG |
denotes |
According |
T9085 |
335-338 |
VBD |
denotes |
was |
T9086 |
300-302 |
IN |
denotes |
to |
T9087 |
303-306 |
DT |
denotes |
the |
T9088 |
307-319 |
NN |
denotes |
manufacturer |
T9089 |
319-321 |
, |
denotes |
, |
T9090 |
321-324 |
DT |
denotes |
the |
T9091 |
325-334 |
NN |
denotes |
immunogen |
T9092 |
339-340 |
DT |
denotes |
a |
T9093 |
351-358 |
NN |
denotes |
peptide |
T9094 |
341-350 |
JJ |
denotes |
synthetic |
T9095 |
359-367 |
VBN |
denotes |
embedded |
T9096 |
368-370 |
IN |
denotes |
in |
T9097 |
371-379 |
NN |
denotes |
sequence |
T9098 |
380-382 |
PRP |
denotes |
we |
T9099 |
383-390 |
VBD |
denotes |
deduced |
T9100 |
391-393 |
TO |
denotes |
to |
T9101 |
394-404 |
VB |
denotes |
correspond |
T9102 |
405-407 |
IN |
denotes |
to |
T9103 |
408-412 |
NN |
denotes |
exon |
T9104 |
413-414 |
CD |
denotes |
3 |
T9105 |
414-415 |
. |
denotes |
. |
T9106 |
415-549 |
sentence |
denotes |
Binding was detected by chemiluminescence ECL kit (Pierce) using a rabbit anti-chicken IgG horseradish peroxidase conjugate (Pierce). |
T9107 |
416-423 |
NN |
denotes |
Binding |
T9108 |
428-436 |
VBN |
denotes |
detected |
T9109 |
424-427 |
VBD |
denotes |
was |
T9110 |
437-439 |
IN |
denotes |
by |
T9111 |
440-457 |
NN |
denotes |
chemiluminescence |
T9112 |
462-465 |
NN |
denotes |
kit |
T9113 |
458-461 |
NN |
denotes |
ECL |
T9114 |
466-467 |
-LRB- |
denotes |
( |
T9115 |
467-473 |
NNP |
denotes |
Pierce |
T9116 |
473-474 |
-RRB- |
denotes |
) |
T9117 |
475-480 |
VBG |
denotes |
using |
T9118 |
481-482 |
DT |
denotes |
a |
T9119 |
530-539 |
NN |
denotes |
conjugate |
T9120 |
483-489 |
NN |
denotes |
rabbit |
T9121 |
490-502 |
JJ |
denotes |
anti-chicken |
T9122 |
503-506 |
NN |
denotes |
IgG |
T9123 |
507-518 |
NN |
denotes |
horseradish |
T9124 |
519-529 |
NN |
denotes |
peroxidase |
T9125 |
540-541 |
-LRB- |
denotes |
( |
T9126 |
541-547 |
NNP |
denotes |
Pierce |
T9127 |
547-548 |
-RRB- |
denotes |
) |
T9128 |
548-549 |
. |
denotes |
. |
R6102 |
T9028 |
T9029 |
nummod |
50,μg |
R6103 |
T9029 |
T9030 |
nsubjpass |
μg,separated |
R6104 |
T9031 |
T9029 |
prep |
of,μg |
R6105 |
T9032 |
T9033 |
compound |
testis,extract |
R6106 |
T9033 |
T9031 |
pobj |
extract,of |
R6107 |
T9034 |
T9029 |
prep |
in,μg |
R6108 |
T9035 |
T9036 |
compound |
RIPA,buffer |
R6109 |
T9036 |
T9034 |
pobj |
buffer,in |
R6110 |
T9037 |
T9030 |
auxpass |
was,separated |
R6111 |
T9038 |
T9030 |
agent |
by,separated |
R6112 |
T9039 |
T9040 |
nummod |
8,% |
R6113 |
T9040 |
T9041 |
compound |
%,PAGE |
R6114 |
T9041 |
T9038 |
pobj |
PAGE,by |
R6115 |
T9042 |
T9041 |
compound |
SDS,PAGE |
R6116 |
T9043 |
T9041 |
punct |
-,PAGE |
R6117 |
T9044 |
T9030 |
cc |
and,separated |
R6118 |
T9045 |
T9030 |
conj |
electrotransferred,separated |
R6119 |
T9046 |
T9045 |
prep |
onto,electrotransferred |
R6120 |
T9047 |
T9048 |
det |
a,membrane |
R6121 |
T9048 |
T9046 |
pobj |
membrane,onto |
R6122 |
T9049 |
T9048 |
compound |
Pure,membrane |
R6123 |
T9050 |
T9048 |
compound |
Nitrocellulose,membrane |
R6124 |
T9051 |
T9052 |
punct |
(,Rad |
R6125 |
T9052 |
T9048 |
parataxis |
Rad,membrane |
R6126 |
T9053 |
T9052 |
compound |
Bio,Rad |
R6127 |
T9054 |
T9052 |
punct |
-,Rad |
R6128 |
T9055 |
T9052 |
punct |
", ",Rad |
R6129 |
T9056 |
T9052 |
npadvmod |
http://www.biorad.com,Rad |
R6130 |
T9057 |
T9052 |
punct |
),Rad |
R6131 |
T9058 |
T9030 |
punct |
.,separated |
R6132 |
T9060 |
T9061 |
det |
The,membrane |
R6133 |
T9061 |
T9062 |
nsubjpass |
membrane,incubated |
R6134 |
T9063 |
T9062 |
auxpass |
was,incubated |
R6135 |
T9064 |
T9062 |
prep |
with,incubated |
R6136 |
T9065 |
T9066 |
det |
a,antibody |
R6137 |
T9066 |
T9064 |
pobj |
antibody,with |
R6138 |
T9067 |
T9068 |
amod |
polyclonal,rabbit |
R6139 |
T9068 |
T9066 |
nmod |
rabbit,antibody |
R6140 |
T9069 |
T9066 |
amod |
anti-human,antibody |
R6141 |
T9070 |
T9066 |
compound |
TRIP13,antibody |
R6142 |
T9071 |
T9072 |
punct |
(,Genway |
R6143 |
T9072 |
T9066 |
parataxis |
Genway,antibody |
R6144 |
T9073 |
T9074 |
nummod |
18,42687 |
R6145 |
T9074 |
T9072 |
dep |
42687,Genway |
R6146 |
T9075 |
T9074 |
punct |
-,42687 |
R6147 |
T9076 |
T9074 |
nummod |
003,42687 |
R6148 |
T9077 |
T9074 |
punct |
-,42687 |
R6149 |
T9078 |
T9072 |
punct |
;,Genway |
R6150 |
T9079 |
T9072 |
punct |
", ",Genway |
R6151 |
T9080 |
T9072 |
npadvmod |
http://www.genwaybio.com,Genway |
R6152 |
T9081 |
T9072 |
punct |
),Genway |
R6153 |
T9082 |
T9062 |
punct |
.,incubated |
R6154 |
T9084 |
T9085 |
prep |
According,was |
R6155 |
T9086 |
T9084 |
prep |
to,According |
R6156 |
T9087 |
T9088 |
det |
the,manufacturer |
R6157 |
T9088 |
T9086 |
pobj |
manufacturer,to |
R6158 |
T9089 |
T9085 |
punct |
", ",was |
R6159 |
T9090 |
T9091 |
det |
the,immunogen |
R6160 |
T9091 |
T9085 |
nsubj |
immunogen,was |
R6161 |
T9092 |
T9093 |
det |
a,peptide |
R6162 |
T9093 |
T9085 |
attr |
peptide,was |
R6163 |
T9094 |
T9093 |
amod |
synthetic,peptide |
R6164 |
T9095 |
T9093 |
acl |
embedded,peptide |
R6165 |
T9096 |
T9095 |
prep |
in,embedded |
R6166 |
T9097 |
T9096 |
pobj |
sequence,in |
R6167 |
T9098 |
T9099 |
nsubj |
we,deduced |
R6168 |
T9099 |
T9097 |
advcl |
deduced,sequence |
R6169 |
T9100 |
T9101 |
aux |
to,correspond |
R6170 |
T9101 |
T9099 |
xcomp |
correspond,deduced |
R6171 |
T9102 |
T9101 |
prep |
to,correspond |
R6172 |
T9103 |
T9102 |
pobj |
exon,to |
R6173 |
T9104 |
T9103 |
nummod |
3,exon |
R6174 |
T9105 |
T9085 |
punct |
.,was |
R6175 |
T9107 |
T9108 |
nsubjpass |
Binding,detected |
R6176 |
T9109 |
T9108 |
auxpass |
was,detected |
R6177 |
T9110 |
T9108 |
prep |
by,detected |
R6178 |
T9111 |
T9112 |
compound |
chemiluminescence,kit |
R6179 |
T9112 |
T9110 |
pobj |
kit,by |
R6180 |
T9113 |
T9112 |
compound |
ECL,kit |
R6181 |
T9114 |
T9115 |
punct |
(,Pierce |
R6182 |
T9115 |
T9112 |
parataxis |
Pierce,kit |
R6183 |
T9116 |
T9115 |
punct |
),Pierce |
R6184 |
T9117 |
T9108 |
advcl |
using,detected |
R6185 |
T9118 |
T9119 |
det |
a,conjugate |
R6186 |
T9119 |
T9117 |
dobj |
conjugate,using |
R6187 |
T9120 |
T9119 |
nmod |
rabbit,conjugate |
R6188 |
T9121 |
T9119 |
amod |
anti-chicken,conjugate |
R6189 |
T9122 |
T9119 |
compound |
IgG,conjugate |
R6190 |
T9123 |
T9124 |
compound |
horseradish,peroxidase |
R6191 |
T9124 |
T9119 |
compound |
peroxidase,conjugate |
R6192 |
T9125 |
T9126 |
punct |
(,Pierce |
R6193 |
T9126 |
T9119 |
parataxis |
Pierce,conjugate |
R6194 |
T9127 |
T9126 |
punct |
),Pierce |
R6195 |
T9128 |
T9108 |
punct |
.,detected |