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PMC:1941754 / 40514-41219 JSONTXT

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Id Subject Object Predicate Lexical cue
T8526 0-5 CD denotes Three
T8527 6-13 NNS denotes primers
T8528 19-23 VBN denotes used
T8529 14-18 VBD denotes were
T8530 24-26 TO denotes to
T8531 27-38 VB denotes distinguish
T8532 39-43 JJ denotes wild
T8533 44-48 NN denotes type
T8534 43-44 HYPH denotes -
T8535 60-67 NNS denotes alleles
T8536 49-52 CC denotes and
T8537 53-59 NN denotes mutant
T8538 68-70 IN denotes of
T8539 71-77 NN denotes Trip13
T8540 77-79 : denotes :
T8541 79-85 NN denotes primer
T8542 86-87 CD denotes 1
T8543 87-89 , denotes ,
T8544 89-90 CD denotes 5
T8545 92-113 NN denotes CGTCGCTCCATTGCTTTGTGC
T8546 90-91 SYM denotes ′
T8547 91-92 HYPH denotes -
T8548 113-114 HYPH denotes -
T8549 114-115 CD denotes 3
T8550 115-116 SYM denotes ′
T8551 116-117 : denotes ;
T8552 118-124 NN denotes primer
T8553 125-126 CD denotes 2
T8554 126-128 , denotes ,
T8555 128-129 CD denotes 5
T8556 131-164 NN denotes AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
T8557 129-130 SYM denotes ′
T8558 130-131 HYPH denotes -
T8559 164-165 HYPH denotes -
T8560 165-166 CD denotes 3
T8561 166-167 SYM denotes ′
T8562 167-168 : denotes ;
T8563 169-172 CC denotes and
T8564 173-179 NN denotes primer
T8565 180-181 CD denotes 3
T8566 181-183 , denotes ,
T8567 183-184 CD denotes 5
T8568 186-209 NN denotes GTAGATCCCGGCGCTCTTACCAA
T8569 184-185 SYM denotes ′
T8570 185-186 HYPH denotes -
T8571 209-210 HYPH denotes -
T8572 210-211 CD denotes 3
T8573 211-212 SYM denotes ′
T8574 212-213 . denotes .
T8575 213-328 sentence denotes Primers 1 and 2 are located upstream and downstream, respectively, of the gene trap insertion within the intron 3.
T8576 214-221 NNS denotes Primers
T8577 222-223 CD denotes 1
T8578 234-241 VBN denotes located
T8579 224-227 CC denotes and
T8580 228-229 CD denotes 2
T8581 230-233 VBP denotes are
T8582 242-250 RB denotes upstream
T8583 251-254 CC denotes and
T8584 255-265 RB denotes downstream
T8585 265-267 , denotes ,
T8586 267-279 RB denotes respectively
T8587 279-281 , denotes ,
T8588 281-283 IN denotes of
T8589 284-287 DT denotes the
T8590 298-307 NN denotes insertion
T8591 288-292 NN denotes gene
T8592 293-297 NN denotes trap
T8593 308-314 IN denotes within
T8594 315-318 DT denotes the
T8595 319-325 NN denotes intron
T8596 326-327 CD denotes 3
T8597 327-328 . denotes .
T8598 328-369 sentence denotes Primer 3 corresponds to pGTlxf sequence.
T8599 329-335 NN denotes Primer
T8600 338-349 VBZ denotes corresponds
T8601 336-337 CD denotes 3
T8602 350-352 IN denotes to
T8603 353-359 NN denotes pGTlxf
T8604 360-368 NN denotes sequence
T8605 368-369 . denotes .
T8606 369-496 sentence denotes Primers 1 and 2 amplify a 700-bp band from a wild-type allele; primers 1 and 3 amplify a 540-bp fragment from a mutant allele.
T8607 370-377 NNS denotes Primers
T8608 378-379 CD denotes 1
T8609 386-393 VBP denotes amplify
T8610 380-383 CC denotes and
T8611 384-385 CD denotes 2
T8612 449-456 VBP denotes amplify
T8613 394-395 DT denotes a
T8614 403-407 NN denotes band
T8615 396-399 CD denotes 700
T8616 400-402 NN denotes bp
T8617 399-400 HYPH denotes -
T8618 408-412 IN denotes from
T8619 413-414 DT denotes a
T8620 425-431 NN denotes allele
T8621 415-419 JJ denotes wild
T8622 420-424 NN denotes type
T8623 419-420 HYPH denotes -
T8624 431-432 : denotes ;
T8625 433-440 NNS denotes primers
T8626 441-442 CD denotes 1
T8627 443-446 CC denotes and
T8628 447-448 CD denotes 3
T8629 457-458 DT denotes a
T8630 466-474 NN denotes fragment
T8631 459-462 CD denotes 540
T8632 463-465 NN denotes bp
T8633 462-463 HYPH denotes -
T8634 475-479 IN denotes from
T8635 480-481 DT denotes a
T8636 489-495 NN denotes allele
T8637 482-488 NN denotes mutant
T8638 495-496 . denotes .
T8639 496-594 sentence denotes Separate reactions were used to assay the presence or absence of each amplicon from a DNA sample.
T8640 497-505 JJ denotes Separate
T8641 506-515 NNS denotes reactions
T8642 521-525 VBN denotes used
T8643 516-520 VBD denotes were
T8644 526-528 TO denotes to
T8645 529-534 VB denotes assay
T8646 535-538 DT denotes the
T8647 539-547 NN denotes presence
T8648 548-550 CC denotes or
T8649 551-558 NN denotes absence
T8650 559-561 IN denotes of
T8651 562-566 DT denotes each
T8652 567-575 NN denotes amplicon
T8653 576-580 IN denotes from
T8654 581-582 DT denotes a
T8655 587-593 NN denotes sample
T8656 583-586 NN denotes DNA
T8657 593-594 . denotes .
T8658 594-705 sentence denotes The cycling conditions were: 94 °C 2 min; 35 cycles of 94 °C 30 s, 57 °C 45 s, and 72°C 50 s; and 72 °C 2 min.
T8659 595-598 DT denotes The
T8660 607-617 NNS denotes conditions
T8661 599-606 NN denotes cycling
T8662 618-622 VBD denotes were
T8663 622-624 : denotes :
T8664 624-626 CD denotes 94
T8665 627-629 NN denotes °C
T8666 630-631 CD denotes 2
T8667 632-635 NN denotes min
T8668 635-636 : denotes ;
T8669 637-639 CD denotes 35
T8670 640-646 NNS denotes cycles
T8671 647-649 IN denotes of
T8672 650-652 CD denotes 94
T8673 653-655 NN denotes °C
T8674 656-658 CD denotes 30
T8675 659-660 NN denotes s
T8676 660-662 , denotes ,
T8677 662-664 CD denotes 57
T8678 665-667 NN denotes °C
T8679 668-670 CD denotes 45
T8680 671-672 NN denotes s
T8681 672-674 , denotes ,
T8682 674-677 CC denotes and
T8683 678-680 CD denotes 72
T8684 680-682 NN denotes °C
T8685 683-685 CD denotes 50
T8686 686-687 NN denotes s
T8687 687-688 : denotes ;
T8688 689-692 CC denotes and
T8689 693-695 CD denotes 72
T8690 696-698 NN denotes °C
T8691 699-700 CD denotes 2
T8692 701-704 NN denotes min
T8693 704-705 . denotes .
R5713 T8526 T8527 nummod Three,primers
R5714 T8527 T8528 nsubjpass primers,used
R5715 T8529 T8528 auxpass were,used
R5716 T8530 T8531 aux to,distinguish
R5717 T8531 T8528 advcl distinguish,used
R5718 T8532 T8533 amod wild,type
R5719 T8533 T8535 nmod type,alleles
R5720 T8534 T8533 punct -,type
R5721 T8535 T8531 dobj alleles,distinguish
R5722 T8536 T8533 cc and,type
R5723 T8537 T8533 conj mutant,type
R5724 T8538 T8535 prep of,alleles
R5725 T8539 T8538 pobj Trip13,of
R5726 T8540 T8535 punct : ,alleles
R5727 T8541 T8535 appos primer,alleles
R5728 T8542 T8541 nummod 1,primer
R5729 T8543 T8541 punct ", ",primer
R5730 T8544 T8545 nummod 5,CGTCGCTCCATTGCTTTGTGC
R5731 T8545 T8541 appos CGTCGCTCCATTGCTTTGTGC,primer
R5732 T8546 T8544 punct ′,5
R5733 T8547 T8545 punct -,CGTCGCTCCATTGCTTTGTGC
R5734 T8548 T8545 punct -,CGTCGCTCCATTGCTTTGTGC
R5735 T8549 T8545 nummod 3,CGTCGCTCCATTGCTTTGTGC
R5736 T8550 T8545 punct ′,CGTCGCTCCATTGCTTTGTGC
R5737 T8551 T8541 punct ;,primer
R5738 T8552 T8541 conj primer,primer
R5739 T8553 T8552 nummod 2,primer
R5740 T8554 T8552 punct ", ",primer
R5741 T8555 T8556 nummod 5,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5742 T8556 T8552 appos AGTAGTGGTACACTGTATTTTTGCTTTCATTGA,primer
R5743 T8557 T8555 punct ′,5
R5744 T8558 T8556 punct -,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5745 T8559 T8556 punct -,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5746 T8560 T8556 nummod 3,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5747 T8561 T8556 punct ′,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5748 T8562 T8552 punct ;,primer
R5749 T8563 T8552 cc and,primer
R5750 T8564 T8552 conj primer,primer
R5751 T8565 T8564 nummod 3,primer
R5752 T8566 T8564 punct ", ",primer
R5753 T8567 T8568 nummod 5,GTAGATCCCGGCGCTCTTACCAA
R5754 T8568 T8564 appos GTAGATCCCGGCGCTCTTACCAA,primer
R5755 T8569 T8567 punct ′,5
R5756 T8570 T8568 punct -,GTAGATCCCGGCGCTCTTACCAA
R5757 T8571 T8568 punct -,GTAGATCCCGGCGCTCTTACCAA
R5758 T8572 T8568 nummod 3,GTAGATCCCGGCGCTCTTACCAA
R5759 T8573 T8568 punct ′,GTAGATCCCGGCGCTCTTACCAA
R5760 T8574 T8528 punct .,used
R5761 T8576 T8577 nmod Primers,1
R5762 T8577 T8578 nsubjpass 1,located
R5763 T8579 T8577 cc and,1
R5764 T8580 T8577 conj 2,1
R5765 T8581 T8578 auxpass are,located
R5766 T8582 T8578 advmod upstream,located
R5767 T8583 T8582 cc and,upstream
R5768 T8584 T8582 conj downstream,upstream
R5769 T8585 T8584 punct ", ",downstream
R5770 T8586 T8584 conj respectively,downstream
R5771 T8587 T8586 punct ", ",respectively
R5772 T8588 T8586 conj of,respectively
R5773 T8589 T8590 det the,insertion
R5774 T8590 T8588 pobj insertion,of
R5775 T8591 T8592 compound gene,trap
R5776 T8592 T8590 compound trap,insertion
R5777 T8593 T8590 prep within,insertion
R5778 T8594 T8595 det the,intron
R5779 T8595 T8593 pobj intron,within
R5780 T8596 T8595 nummod 3,intron
R5781 T8597 T8578 punct .,located
R5782 T8599 T8600 nsubj Primer,corresponds
R5783 T8601 T8599 nummod 3,Primer
R5784 T8602 T8600 prep to,corresponds
R5785 T8603 T8604 compound pGTlxf,sequence
R5786 T8604 T8602 pobj sequence,to
R5787 T8605 T8600 punct .,corresponds
R5788 T8607 T8608 nmod Primers,1
R5789 T8608 T8609 nsubj 1,amplify
R5790 T8609 T8612 ccomp amplify,amplify
R5791 T8610 T8608 cc and,1
R5792 T8611 T8608 conj 2,1
R5793 T8613 T8614 det a,band
R5794 T8614 T8609 dobj band,amplify
R5795 T8615 T8616 nummod 700,bp
R5796 T8616 T8614 compound bp,band
R5797 T8617 T8616 punct -,bp
R5798 T8618 T8614 prep from,band
R5799 T8619 T8620 det a,allele
R5800 T8620 T8618 pobj allele,from
R5801 T8621 T8622 amod wild,type
R5802 T8622 T8620 compound type,allele
R5803 T8623 T8622 punct -,type
R5804 T8624 T8612 punct ;,amplify
R5805 T8625 T8626 nmod primers,1
R5806 T8626 T8612 nsubj 1,amplify
R5807 T8627 T8626 cc and,1
R5808 T8628 T8626 conj 3,1
R5809 T8629 T8630 det a,fragment
R5810 T8630 T8612 dobj fragment,amplify
R5811 T8631 T8632 nummod 540,bp
R5812 T8632 T8630 compound bp,fragment
R5813 T8633 T8632 punct -,bp
R5814 T8634 T8630 prep from,fragment
R5815 T8635 T8636 det a,allele
R5816 T8636 T8634 pobj allele,from
R5817 T8637 T8636 compound mutant,allele
R5818 T8638 T8612 punct .,amplify
R5819 T8640 T8641 amod Separate,reactions
R5820 T8641 T8642 nsubjpass reactions,used
R5821 T8643 T8642 auxpass were,used
R5822 T8644 T8645 aux to,assay
R5823 T8645 T8642 advcl assay,used
R5824 T8646 T8647 det the,presence
R5825 T8647 T8645 dobj presence,assay
R5826 T8648 T8647 cc or,presence
R5827 T8649 T8647 conj absence,presence
R5828 T8650 T8647 prep of,presence
R5829 T8651 T8652 det each,amplicon
R5830 T8652 T8650 pobj amplicon,of
R5831 T8653 T8645 prep from,assay
R5832 T8654 T8655 det a,sample
R5833 T8655 T8653 pobj sample,from
R5834 T8656 T8655 compound DNA,sample
R5835 T8657 T8642 punct .,used
R5836 T8659 T8660 det The,conditions
R5837 T8660 T8662 nsubj conditions,were
R5838 T8661 T8660 compound cycling,conditions
R5839 T8663 T8662 punct : ,were
R5840 T8664 T8665 nummod 94,°C
R5841 T8665 T8662 attr °C,were
R5842 T8666 T8667 nummod 2,min
R5843 T8667 T8665 npadvmod min,°C
R5844 T8668 T8665 punct ;,°C
R5845 T8669 T8670 nummod 35,cycles
R5846 T8670 T8665 conj cycles,°C
R5847 T8671 T8670 prep of,cycles
R5848 T8672 T8673 nummod 94,°C
R5849 T8673 T8671 pobj °C,of
R5850 T8674 T8675 nummod 30,s
R5851 T8675 T8670 npadvmod s,cycles
R5852 T8676 T8670 punct ", ",cycles
R5853 T8677 T8678 nummod 57,°C
R5854 T8678 T8670 conj °C,cycles
R5855 T8679 T8680 nummod 45,s
R5856 T8680 T8678 npadvmod s,°C
R5857 T8681 T8678 punct ", ",°C
R5858 T8682 T8678 cc and,°C
R5859 T8683 T8684 nummod 72,°C
R5860 T8684 T8678 conj °C,°C
R5861 T8685 T8686 nummod 50,s
R5862 T8686 T8684 npadvmod s,°C
R5863 T8687 T8670 punct ;,cycles
R5864 T8688 T8670 cc and,cycles
R5865 T8689 T8690 nummod 72,°C
R5866 T8690 T8670 conj °C,cycles
R5867 T8691 T8692 nummod 2,min
R5868 T8692 T8690 npadvmod min,°C
R5869 T8693 T8662 punct .,were

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T8479 6-13 SO:0000112 denotes primers
T8480 60-67 SO:0001023 denotes alleles
T8481 71-77 PR:000016672 denotes Trip13
T8482 79-85 SO:0000112 denotes primer
T8483 118-124 SO:0000112 denotes primer
T8484 173-179 SO:0000112 denotes primer
T8485 214-221 SO:0000112 denotes Primers
T8486 288-292 SO:0000704 denotes gene
T8487 319-325 SO:0000188 denotes intron
T8488 329-335 SO:0000112 denotes Primer
T8489 370-377 SO:0000112 denotes Primers
T8490 400-402 SO:0000028 denotes bp
T8491 425-431 SO:0001023 denotes allele
T8492 433-440 SO:0000112 denotes primers
T8493 463-465 SO:0000028 denotes bp
T8494 489-495 SO:0001023 denotes allele

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T8496 6-13 SO_EXT:0000112 denotes primers
T8497 39-48 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8498 53-59 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8499 60-67 SO_EXT:0001023 denotes alleles
T8500 71-77 PR_EXT:000016672 denotes Trip13
T8501 79-85 SO_EXT:0000112 denotes primer
T8502 118-124 SO_EXT:0000112 denotes primer
T8503 173-179 SO_EXT:0000112 denotes primer
T8504 214-221 SO_EXT:0000112 denotes Primers
T8505 242-250 SO_EXT:sequence_upstreamness denotes upstream
T8506 255-265 SO_EXT:sequence_downstreamness denotes downstream
T8507 288-297 SO_EXT:gene_trap_or_trap_construct denotes gene trap
T8508 298-307 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T8509 319-325 SO_EXT:0000188 denotes intron
T8510 329-335 SO_EXT:0000112 denotes Primer
T8511 360-368 SO_EXT:biological_sequence denotes sequence
T8512 370-377 SO_EXT:0000112 denotes Primers
T8513 400-402 SO_EXT:0000028 denotes bp
T8514 415-424 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8515 425-431 SO_EXT:0001023 denotes allele
T8516 433-440 SO_EXT:0000112 denotes primers
T8517 463-465 SO_EXT:0000028 denotes bp
T8518 482-488 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8519 489-495 SO_EXT:0001023 denotes allele
T8520 583-586 CHEBI_SO_EXT:DNA denotes DNA