PMC:1941754 / 28883-29905 JSONTXT

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Immunocytological analysis indicated that both Dmc1 −/− and Dmc1 −/− Trip13Gt/Gt chromosomes had extensive asynapsis compared to Trip13Gt single mutants (Figure 5F–5H), and all had persistent RAD51/DMC1 foci and phosphorylated H2AX (γH2AX; Figure 5I–5L), confirming that Dmc1 is epistatic to Trip13. 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