PMC:1920263 / 15340-17307
Annnotations
test2
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T5934 | 464-484 | Protein | denotes | Micrococcal Nuclease |
| T5935 | 778-781 | Protein | denotes | Sp1 |
| T5936 | 795-798 | Protein | denotes | Sp3 |
| T5937 | 815-820 | Protein | denotes | actin |
| T5938 | 1209-1212 | Protein | denotes | A3G |
| T5939 | 1456-1470 | Protein | denotes | Taq polymerase |
| T5940 | 1683-1686 | Protein | denotes | A3G |
| T5941 | 1876-1879 | Protein | denotes | A3G |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T6743 | 0-9 | http://purl.obolibrary.org/obo/GO_0000785 | denotes | Chromatin |
| T6744 | 649-658 | http://purl.obolibrary.org/obo/GO_0000785 | denotes | Chromatin |
| T6745 | 49-54 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T6746 | 509-514 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T6747 | 753-758 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T6748 | 768-776 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
| T6749 | 768-776 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T6742 | 768-776 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T5972 | 242-247 | http://purl.obolibrary.org/obo/GO_0019835 | denotes | lysis |
| T5973 | 429-438 | http://purl.obolibrary.org/obo/GO_0007586 | denotes | digestion |
UBERON-AE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T5942 | 519-523 | http://purl.obolibrary.org/obo/UBERON_0000025 | denotes | tube |
bionlp-st-ge-2016-spacy-parsed
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T6333 | 0-9 | NNP | denotes | Chromatin |
| T6334 | 10-29 | NN | denotes | immunoprecipitation |
| T6335 | 30-31 | -LRB- | denotes | ( |
| T6336 | 31-35 | NN | denotes | ChIP |
| T6337 | 35-36 | -RRB- | denotes | ) |
| T6338 | 37-42 | NN | denotes | assay |
| T6339 | 43-45 | CD | denotes | A3 |
| T6340 | 45-48 | CD | denotes | .01 |
| T6341 | 49-54 | NNS | denotes | cells |
| T6342 | 55-59 | VBD | denotes | were |
| T6343 | 60-67 | VBN | denotes | treated |
| T6344 | 68-72 | IN | denotes | with |
| T6345 | 73-77 | NNP | denotes | RPMI |
| T6346 | 78-85 | NN | denotes | culture |
| T6347 | 86-92 | NN | denotes | medium |
| T6348 | 93-103 | VBG | denotes | containing |
| T6349 | 104-105 | CD | denotes | 1 |
| T6350 | 105-106 | NN | denotes | % |
| T6351 | 107-119 | NN | denotes | formaldehyde |
| T6352 | 120-123 | IN | denotes | for |
| T6353 | 124-126 | CD | denotes | 10 |
| T6354 | 127-130 | NN | denotes | min |
| T6355 | 130-131 | . | denotes | . |
| T6356 | 132-134 | IN | denotes | at |
| T6357 | 135-137 | CD | denotes | 37 |
| T6358 | 137-138 | NN | denotes | ° |
| T6359 | 138-140 | NNP | denotes | C. |
| T6360 | 141-146 | NNP | denotes | Cells |
| T6361 | 147-151 | VBD | denotes | were |
| T6362 | 152-158 | VBN | denotes | washed |
| T6363 | 159-164 | RB | denotes | twice |
| T6364 | 165-169 | IN | denotes | with |
| T6365 | 170-178 | JJ | denotes | ice-cold |
| T6366 | 179-182 | NNP | denotes | PBS |
| T6367 | 183-186 | CC | denotes | and |
| T6368 | 187-196 | VBN | denotes | incubated |
| T6369 | 197-200 | IN | denotes | for |
| T6370 | 201-203 | CD | denotes | 10 |
| T6371 | 204-207 | NN | denotes | min |
| T6372 | 207-208 | . | denotes | . |
| T6373 | 209-211 | IN | denotes | on |
| T6374 | 212-215 | NN | denotes | ice |
| T6375 | 216-221 | IN | denotes | after |
| T6376 | 222-234 | NN | denotes | resuspension |
| T6377 | 235-237 | IN | denotes | in |
| T6378 | 238-241 | NNP | denotes | SDS |
| T6379 | 242-247 | VBZ | denotes | lysis |
| T6380 | 248-254 | NN | denotes | buffer |
| T6381 | 255-256 | -LRB- | denotes | ( |
| T6382 | 256-260 | NNP | denotes | ChIP |
| T6383 | 261-266 | NNP | denotes | Assay |
| T6384 | 267-270 | NNP | denotes | Kit |
| T6385 | 270-271 | , | denotes | , |
| T6386 | 272-279 | NNP | denotes | Upstate |
| T6387 | 279-280 | -RRB- | denotes | ) |
| T6388 | 280-281 | . | denotes | . |
| T6389 | 282-287 | IN | denotes | After |
| T6390 | 288-302 | NN | denotes | centrifugation |
| T6391 | 302-303 | , | denotes | , |
| T6392 | 304-311 | NNS | denotes | pellets |
| T6393 | 312-316 | VBD | denotes | were |
| T6394 | 317-328 | VBN | denotes | resuspended |
| T6395 | 329-331 | IN | denotes | in |
| T6396 | 332-337 | NNP | denotes | MNase |
| T6397 | 338-346 | NN | denotes | reaction |
| T6398 | 347-353 | NN | denotes | buffer |
| T6399 | 354-355 | -LRB- | denotes | ( |
| T6400 | 355-357 | CD | denotes | 10 |
| T6401 | 358-360 | NNP | denotes | mM |
| T6402 | 361-369 | NNP | denotes | Tris-HCl |
| T6403 | 370-372 | NNP | denotes | pH |
| T6404 | 373-376 | CD | denotes | 7.5 |
| T6405 | 376-377 | , | denotes | , |
| T6406 | 378-380 | CD | denotes | 10 |
| T6407 | 381-383 | NNP | denotes | mM |
| T6408 | 384-388 | NNP | denotes | NaCl |
| T6409 | 388-389 | , | denotes | , |
| T6410 | 390-391 | CD | denotes | 3 |
| T6411 | 392-394 | NNP | denotes | mM |
| T6412 | 395-400 | NNP | denotes | MgCl2 |
| T6413 | 400-401 | , | denotes | , |
| T6414 | 402-403 | CD | denotes | 1 |
| T6415 | 404-406 | NNP | denotes | mM |
| T6416 | 407-412 | NNP | denotes | CaCl2 |
| T6417 | 412-413 | , | denotes | , |
| T6418 | 414-415 | CD | denotes | 4 |
| T6419 | 415-416 | NN | denotes | % |
| T6420 | 417-422 | NN | denotes | NP-40 |
| T6421 | 422-423 | -RRB- | denotes | ) |
| T6422 | 423-424 | . | denotes | . |
| T6423 | 425-428 | NNP | denotes | DNA |
| T6424 | 429-438 | NN | denotes | digestion |
| T6425 | 439-442 | VBD | denotes | was |
| T6426 | 443-452 | VBN | denotes | performed |
| T6427 | 453-458 | VBG | denotes | using |
| T6428 | 459-461 | CD | denotes | 50 |
| T6429 | 462-463 | NNP | denotes | U |
| T6430 | 464-475 | NNP | denotes | Micrococcal |
| T6431 | 476-484 | NNP | denotes | Nuclease |
| T6432 | 485-486 | -LRB- | denotes | ( |
| T6433 | 486-495 | NNP | denotes | Fermentas |
| T6434 | 495-496 | -RRB- | denotes | ) |
| T6435 | 497-500 | CC | denotes | and |
| T6436 | 501-502 | CD | denotes | 1 |
| T6437 | 503-504 | CD | denotes | × |
| T6438 | 505-508 | CD | denotes | 107 |
| T6439 | 509-514 | NNS | denotes | cells |
| T6440 | 515-518 | IN | denotes | per |
| T6441 | 519-523 | NN | denotes | tube |
| T6442 | 524-526 | IN | denotes | in |
| T6443 | 527-528 | DT | denotes | a |
| T6444 | 529-535 | NN | denotes | volume |
| T6445 | 536-538 | IN | denotes | of |
| T6446 | 539-542 | CD | denotes | 1.5 |
| T6447 | 543-545 | NN | denotes | ml |
| T6448 | 545-546 | . | denotes | . |
| T6449 | 547-552 | IN | denotes | After |
| T6450 | 553-554 | CD | denotes | 2 |
| T6451 | 555-558 | NN | denotes | min |
| T6452 | 558-559 | , | denotes | , |
| T6453 | 560-568 | NN | denotes | reaction |
| T6454 | 569-572 | VBD | denotes | was |
| T6455 | 573-580 | VBN | denotes | stopped |
| T6456 | 581-583 | IN | denotes | by |
| T6457 | 584-590 | VBG | denotes | adding |
| T6458 | 591-593 | CD | denotes | 30 |
| T6459 | 594-596 | NN | denotes | µl |
| T6460 | 597-600 | CD | denotes | 200 |
| T6461 | 601-603 | NNP | denotes | mM |
| T6462 | 604-608 | NNP | denotes | EGTA |
| T6463 | 608-609 | . | denotes | . |
| T6464 | 610-617 | JJ | denotes | Further |
| T6465 | 618-623 | NNS | denotes | steps |
| T6466 | 624-628 | VBD | denotes | were |
| T6467 | 629-638 | VBN | denotes | performed |
| T6468 | 639-644 | VBG | denotes | using |
| T6469 | 645-648 | DT | denotes | the |
| T6470 | 649-658 | NNP | denotes | Chromatin |
| T6471 | 659-678 | NNP | denotes | Immunoprecipitation |
| T6472 | 679-684 | NNP | denotes | Assay |
| T6473 | 685-688 | NNP | denotes | Kit |
| T6474 | 689-690 | -LRB- | denotes | ( |
| T6475 | 690-697 | NNP | denotes | Upstate |
| T6476 | 697-698 | -RRB- | denotes | ) |
| T6477 | 699-708 | VBG | denotes | according |
| T6478 | 709-711 | TO | denotes | to |
| T6479 | 712-715 | DT | denotes | the |
| T6480 | 716-728 | NN | denotes | manufacturer |
| T6481 | 728-730 | POS | denotes | 's |
| T6482 | 731-743 | NNS | denotes | instructions |
| T6483 | 743-744 | . | denotes | . |
| T6484 | 745-746 | CD | denotes | 1 |
| T6485 | 747-748 | CD | denotes | × |
| T6486 | 749-752 | CD | denotes | 107 |
| T6487 | 753-758 | NNS | denotes | cells |
| T6488 | 759-762 | CC | denotes | and |
| T6489 | 763-764 | CD | denotes | 2 |
| T6490 | 765-767 | NN | denotes | µg |
| T6491 | 768-776 | NN | denotes | antibody |
| T6492 | 777-778 | -LRB- | denotes | ( |
| T6493 | 778-781 | NNP | denotes | Sp1 |
| T6494 | 781-782 | -LRB- | denotes | ( |
| T6495 | 782-786 | NNP | denotes | Pep2 |
| T6496 | 786-787 | -RRB- | denotes | ) |
| T6497 | 788-793 | JJ | denotes | sc-59 |
| T6498 | 793-794 | , | denotes | , |
| T6499 | 795-798 | JJ | denotes | Sp3 |
| T6500 | 798-799 | -LRB- | denotes | ( |
| T6501 | 799-803 | NN | denotes | D-20 |
| T6502 | 803-804 | -RRB- | denotes | ) |
| T6503 | 805-811 | JJ | denotes | sc-644 |
| T6504 | 812-814 | CC | denotes | or |
| T6505 | 815-820 | NN | denotes | actin |
| T6506 | 820-821 | -LRB- | denotes | ( |
| T6507 | 821-826 | NNP | denotes | H-196 |
| T6508 | 826-827 | -RRB- | denotes | ) |
| T6509 | 828-835 | JJ | denotes | sc-7210 |
| T6510 | 835-836 | , | denotes | , |
| T6511 | 837-842 | NNP | denotes | Santa |
| T6512 | 843-847 | NNP | denotes | Cruz |
| T6513 | 848-861 | NNP | denotes | Biotechnology |
| T6514 | 861-862 | -RRB- | denotes | ) |
| T6515 | 863-867 | VBD | denotes | were |
| T6516 | 868-872 | VBN | denotes | used |
| T6517 | 873-876 | IN | denotes | for |
| T6518 | 877-881 | DT | denotes | each |
| T6519 | 882-901 | NN | denotes | immunoprecipitation |
| T6520 | 901-902 | . | denotes | . |
| T6521 | 903-906 | DT | denotes | All |
| T6522 | 907-914 | NNS | denotes | buffers |
| T6523 | 915-919 | VBD | denotes | were |
| T6524 | 920-927 | RB | denotes | freshly |
| T6525 | 928-936 | VBN | denotes | supplied |
| T6526 | 937-941 | IN | denotes | with |
| T6527 | 942-950 | NN | denotes | protease |
| T6528 | 951-961 | NNS | denotes | inhibitors |
| T6529 | 961-962 | . | denotes | . |
| T6530 | 963-968 | IN | denotes | After |
| T6531 | 969-986 | NN | denotes | phenol/chloroform |
| T6532 | 987-997 | NN | denotes | extraction |
| T6533 | 998-1001 | CC | denotes | and |
| T6534 | 1002-1009 | NN | denotes | ethanol |
| T6535 | 1010-1023 | NN | denotes | precipitation |
| T6536 | 1023-1024 | , | denotes | , |
| T6537 | 1025-1028 | NNP | denotes | DNA |
| T6538 | 1029-1032 | VBD | denotes | was |
| T6539 | 1033-1041 | VBN | denotes | resolved |
| T6540 | 1042-1044 | IN | denotes | in |
| T6541 | 1045-1047 | CD | denotes | 16 |
| T6542 | 1048-1050 | NN | denotes | µl |
| T6543 | 1051-1054 | CD | denotes | H2O |
| T6544 | 1054-1055 | . | denotes | . |
| T6545 | 1056-1074 | NNP | denotes | Immunoprecipitated |
| T6546 | 1075-1078 | NNP | denotes | DNA |
| T6547 | 1079-1082 | VBD | denotes | was |
| T6548 | 1083-1091 | VBN | denotes | detected |
| T6549 | 1092-1094 | IN | denotes | by |
| T6550 | 1095-1101 | JJ | denotes | nested |
| T6551 | 1102-1105 | NNP | denotes | PCR |
| T6552 | 1106-1111 | VBG | denotes | using |
| T6553 | 1112-1113 | CD | denotes | 4 |
| T6554 | 1114-1116 | NN | denotes | µl |
| T6555 | 1117-1119 | IN | denotes | of |
| T6556 | 1120-1123 | DT | denotes | the |
| T6557 | 1124-1132 | VBN | denotes | resolved |
| T6558 | 1133-1136 | NN | denotes | DNA |
| T6559 | 1137-1139 | IN | denotes | in |
| T6560 | 1140-1143 | DT | denotes | the |
| T6561 | 1144-1149 | JJ | denotes | first |
| T6562 | 1150-1153 | NNP | denotes | PCR |
| T6563 | 1153-1154 | , | denotes | , |
| T6564 | 1155-1158 | CC | denotes | and |
| T6565 | 1159-1160 | CD | denotes | 1 |
| T6566 | 1161-1163 | NN | denotes | µl |
| T6567 | 1164-1167 | IN | denotes | for |
| T6568 | 1168-1171 | DT | denotes | the |
| T6569 | 1172-1178 | JJ | denotes | nested |
| T6570 | 1179-1182 | NNP | denotes | PCR |
| T6571 | 1182-1183 | . | denotes | . |
| T6572 | 1184-1187 | IN | denotes | For |
| T6573 | 1188-1201 | NN | denotes | amplification |
| T6574 | 1202-1204 | IN | denotes | of |
| T6575 | 1205-1208 | DT | denotes | the |
| T6576 | 1209-1212 | NNP | denotes | A3G |
| T6577 | 1213-1221 | NN | denotes | promoter |
| T6578 | 1221-1222 | , | denotes | , |
| T6579 | 1223-1226 | DT | denotes | the |
| T6580 | 1227-1236 | VBG | denotes | following |
| T6581 | 1237-1244 | NNS | denotes | primers |
| T6582 | 1245-1249 | VBD | denotes | were |
| T6583 | 1250-1254 | VBN | denotes | used |
| T6584 | 1254-1255 | : | denotes | : |
| T6585 | 1256-1266 | JJ | denotes | ChIP3Gplus |
| T6586 | 1267-1268 | CD | denotes | 5 |
| T6587 | 1268-1269 | SYM | denotes | ′ |
| T6588 | 1269-1270 | : | denotes | - |
| T6589 | 1270-1294 | JJ | denotes | ccacggtggcctccgagggtga-3 |
| T6590 | 1294-1295 | NN | denotes | ′ |
| T6591 | 1296-1299 | CC | denotes | and |
| T6592 | 1300-1311 | NNS | denotes | ChIP3Gminus |
| T6593 | 1311-1312 | : | denotes | : |
| T6594 | 1313-1314 | CD | denotes | 5 |
| T6595 | 1314-1315 | SYM | denotes | ′ |
| T6596 | 1315-1316 | : | denotes | - |
| T6597 | 1316-1339 | JJ | denotes | ctctccaccatcaagacagac-3 |
| T6598 | 1339-1340 | NN | denotes | ′ |
| T6599 | 1341-1342 | -LRB- | denotes | ( |
| T6600 | 1342-1343 | CD | denotes | 1 |
| T6601 | 1343-1344 | . | denotes | . |
| T6602 | 1345-1348 | NNP | denotes | PCR |
| T6603 | 1348-1349 | -RRB- | denotes | ) |
| T6604 | 1349-1350 | : | denotes | ; |
| T6605 | 1351-1362 | NNS | denotes | ChIP3G2plus |
| T6606 | 1362-1363 | : | denotes | : |
| T6607 | 1364-1365 | CD | denotes | 5 |
| T6608 | 1365-1366 | SYM | denotes | ′ |
| T6609 | 1366-1367 | : | denotes | - |
| T6610 | 1367-1390 | JJ | denotes | tactctccctccctgtcccca-3 |
| T6611 | 1390-1391 | NN | denotes | ′ |
| T6612 | 1392-1395 | CC | denotes | and |
| T6613 | 1396-1402 | NNP | denotes | ChIP3G |
| T6614 | 1403-1409 | VBD | denotes | nested |
| T6615 | 1410-1415 | CC | denotes | minus |
| T6616 | 1415-1416 | : | denotes | : |
| T6617 | 1417-1418 | CD | denotes | 5 |
| T6618 | 1418-1419 | SYM | denotes | ′ |
| T6619 | 1419-1420 | : | denotes | - |
| T6620 | 1420-1440 | JJ | denotes | aggctgatgcctccgcag-3 |
| T6621 | 1440-1441 | NN | denotes | ′ |
| T6622 | 1442-1443 | -LRB- | denotes | ( |
| T6623 | 1443-1449 | JJ | denotes | nested |
| T6624 | 1450-1453 | NNP | denotes | PCR |
| T6625 | 1453-1454 | -RRB- | denotes | ) |
| T6626 | 1454-1455 | . | denotes | . |
| T6627 | 1456-1459 | NNP | denotes | Taq |
| T6628 | 1460-1470 | NN | denotes | polymerase |
| T6629 | 1471-1472 | -LRB- | denotes | ( |
| T6630 | 1472-1478 | NNP | denotes | Qiagen |
| T6631 | 1478-1479 | -RRB- | denotes | ) |
| T6632 | 1480-1483 | VBD | denotes | was |
| T6633 | 1484-1488 | VBN | denotes | used |
| T6634 | 1489-1497 | RB | denotes | together |
| T6635 | 1498-1502 | IN | denotes | with |
| T6636 | 1503-1506 | DT | denotes | the |
| T6637 | 1507-1516 | JJ | denotes | following |
| T6638 | 1517-1522 | NN | denotes | cycle |
| T6639 | 1523-1533 | NNS | denotes | conditions |
| T6640 | 1533-1534 | : | denotes | : |
| T6641 | 1535-1538 | CD | denotes | one |
| T6642 | 1539-1544 | NN | denotes | cycle |
| T6643 | 1545-1547 | CD | denotes | 94 |
| T6644 | 1547-1548 | NN | denotes | ° |
| T6645 | 1548-1549 | NNP | denotes | C |
| T6646 | 1550-1553 | IN | denotes | for |
| T6647 | 1554-1555 | CD | denotes | 2 |
| T6648 | 1556-1559 | NN | denotes | min |
| T6649 | 1559-1560 | : | denotes | ; |
| T6650 | 1561-1563 | CD | denotes | 30 |
| T6651 | 1564-1570 | NNS | denotes | cycles |
| T6652 | 1571-1573 | CD | denotes | 94 |
| T6653 | 1573-1574 | NN | denotes | ° |
| T6654 | 1574-1575 | NNP | denotes | C |
| T6655 | 1576-1579 | IN | denotes | for |
| T6656 | 1580-1582 | CD | denotes | 30 |
| T6657 | 1583-1584 | PRP | denotes | s |
| T6658 | 1584-1585 | , | denotes | , |
| T6659 | 1586-1588 | CD | denotes | 60 |
| T6660 | 1588-1589 | NN | denotes | ° |
| T6661 | 1589-1590 | NNP | denotes | C |
| T6662 | 1591-1594 | IN | denotes | for |
| T6663 | 1595-1597 | CD | denotes | 60 |
| T6664 | 1598-1599 | PRP | denotes | s |
| T6665 | 1599-1600 | , | denotes | , |
| T6666 | 1601-1603 | CD | denotes | 72 |
| T6667 | 1603-1604 | NN | denotes | ° |
| T6668 | 1604-1605 | NNP | denotes | C |
| T6669 | 1606-1609 | IN | denotes | for |
| T6670 | 1610-1611 | CD | denotes | 2 |
| T6671 | 1612-1615 | NN | denotes | min |
| T6672 | 1615-1616 | : | denotes | ; |
| T6673 | 1617-1620 | CD | denotes | one |
| T6674 | 1621-1626 | NN | denotes | cycle |
| T6675 | 1627-1629 | CD | denotes | 72 |
| T6676 | 1629-1630 | NN | denotes | ° |
| T6677 | 1630-1631 | NNP | denotes | C |
| T6678 | 1632-1635 | IN | denotes | for |
| T6679 | 1636-1638 | CD | denotes | 10 |
| T6680 | 1639-1642 | NN | denotes | min |
| T6681 | 1642-1643 | . | denotes | . |
| T6682 | 1644-1646 | IN | denotes | As |
| T6683 | 1647-1648 | DT | denotes | a |
| T6684 | 1649-1657 | JJ | denotes | negative |
| T6685 | 1658-1665 | NN | denotes | control |
| T6686 | 1665-1666 | , | denotes | , |
| T6687 | 1667-1668 | DT | denotes | a |
| T6688 | 1669-1675 | NN | denotes | region |
| T6689 | 1676-1678 | IN | denotes | in |
| T6690 | 1679-1682 | DT | denotes | the |
| T6691 | 1683-1686 | NNP | denotes | A3G |
| T6692 | 1687-1691 | NN | denotes | gene |
| T6693 | 1692-1695 | VBD | denotes | was |
| T6694 | 1696-1704 | VBN | denotes | targeted |
| T6695 | 1705-1710 | VBG | denotes | using |
| T6696 | 1711-1714 | DT | denotes | the |
| T6697 | 1715-1722 | NNS | denotes | primers |
| T6698 | 1723-1737 | NNS | denotes | ChIP3Gneg_plus |
| T6699 | 1737-1738 | : | denotes | : |
| T6700 | 1739-1740 | CD | denotes | 5 |
| T6701 | 1740-1741 | SYM | denotes | ′ |
| T6702 | 1741-1742 | : | denotes | - |
| T6703 | 1742-1765 | JJ | denotes | taagtaccacccagagatgag-3 |
| T6704 | 1765-1766 | NN | denotes | ′ |
| T6705 | 1767-1770 | CC | denotes | and |
| T6706 | 1771-1786 | NNS | denotes | ChIP3Gneg_minus |
| T6707 | 1786-1787 | : | denotes | : |
| T6708 | 1788-1789 | CD | denotes | 5 |
| T6709 | 1789-1790 | SYM | denotes | ′ |
| T6710 | 1790-1791 | : | denotes | - |
| T6711 | 1791-1814 | JJ | denotes | catgatcttcatggtggcacg-3 |
| T6712 | 1814-1815 | NN | denotes | ′ |
| T6713 | 1816-1819 | IN | denotes | for |
| T6714 | 1820-1824 | DT | denotes | both |
| T6715 | 1825-1828 | NNP | denotes | PCR |
| T6716 | 1829-1834 | NNS | denotes | steps |
| T6717 | 1834-1835 | . | denotes | . |
| T6718 | 1836-1839 | NNP | denotes | PCR |
| T6719 | 1840-1850 | NNS | denotes | conditions |
| T6720 | 1851-1855 | VBD | denotes | were |
| T6721 | 1856-1859 | DT | denotes | the |
| T6722 | 1860-1864 | JJ | denotes | same |
| T6723 | 1865-1867 | IN | denotes | as |
| T6724 | 1868-1871 | IN | denotes | for |
| T6725 | 1872-1875 | DT | denotes | the |
| T6726 | 1876-1879 | NNP | denotes | A3G |
| T6727 | 1880-1888 | NN | denotes | promoter |
| T6728 | 1889-1897 | NN | denotes | sequence |
| T6729 | 1897-1898 | , | denotes | , |
| T6730 | 1899-1903 | IN | denotes | with |
| T6731 | 1904-1907 | DT | denotes | the |
| T6732 | 1908-1917 | NN | denotes | exception |
| T6733 | 1918-1922 | WDT | denotes | that |
| T6734 | 1923-1932 | VBG | denotes | annealing |
| T6735 | 1933-1944 | NN | denotes | temperature |
| T6736 | 1945-1948 | VBD | denotes | was |
| T6737 | 1949-1958 | VBN | denotes | decreased |
| T6738 | 1959-1961 | TO | denotes | to |
| T6739 | 1962-1964 | CD | denotes | 55 |
| T6740 | 1964-1965 | CD | denotes | ° |
| T6741 | 1965-1967 | NNP | denotes | C. |
| R5242 | T6333 | T6334 | compound | Chromatin,immunoprecipitation |
| R5243 | T6334 | T6334 | ROOT | immunoprecipitation,immunoprecipitation |
| R5244 | T6335 | T6336 | punct | (,ChIP |
| R5245 | T6336 | T6334 | appos | ChIP,immunoprecipitation |
| R5246 | T6337 | T6336 | punct | ),ChIP |
| R5247 | T6338 | T6343 | nsubjpass | assay,treated |
| R5248 | T6339 | T6338 | nummod | A3,assay |
| R5249 | T6340 | T6341 | nummod | .01,cells |
| R5250 | T6341 | T6343 | nsubjpass | cells,treated |
| R5251 | T6342 | T6343 | auxpass | were,treated |
| R5252 | T6343 | T6343 | ROOT | treated,treated |
| R5253 | T6344 | T6343 | prep | with,treated |
| R5254 | T6345 | T6347 | compound | RPMI,medium |
| R5255 | T6346 | T6347 | compound | culture,medium |
| R5256 | T6347 | T6344 | pobj | medium,with |
| R5257 | T6348 | T6344 | pcomp | containing,with |
| R5258 | T6349 | T6350 | nummod | 1,% |
| R5259 | T6350 | T6351 | compound | %,formaldehyde |
| R5260 | T6351 | T6348 | dobj | formaldehyde,containing |
| R5261 | T6352 | T6348 | prep | for,containing |
| R5262 | T6353 | T6354 | nummod | 10,min |
| R5263 | T6354 | T6352 | pobj | min,for |
| R5264 | T6355 | T6343 | punct | .,treated |
| R5265 | T6356 | T6362 | prep | at,washed |
| R5266 | T6357 | T6360 | nummod | 37,Cells |
| R5267 | T6358 | T6360 | compound | °,Cells |
| R5268 | T6359 | T6360 | compound | C.,Cells |
| R5269 | T6360 | T6362 | nsubjpass | Cells,washed |
| R5270 | T6361 | T6362 | auxpass | were,washed |
| R5271 | T6362 | T6362 | ROOT | washed,washed |
| R5272 | T6363 | T6362 | advmod | twice,washed |
| R5273 | T6364 | T6362 | prep | with,washed |
| R5274 | T6365 | T6366 | amod | ice-cold,PBS |
| R5275 | T6366 | T6364 | pobj | PBS,with |
| R5276 | T6367 | T6362 | cc | and,washed |
| R5277 | T6368 | T6362 | conj | incubated,washed |
| R5278 | T6369 | T6368 | prep | for,incubated |
| R5279 | T6370 | T6371 | nummod | 10,min |
| R5280 | T6371 | T6369 | pobj | min,for |
| R5281 | T6372 | T6362 | punct | .,washed |
| R5282 | T6373 | T6373 | ROOT | on,on |
| R5283 | T6374 | T6373 | pobj | ice,on |
| R5284 | T6375 | T6374 | prep | after,ice |
| R5285 | T6376 | T6375 | pobj | resuspension,after |
| R5286 | T6377 | T6376 | prep | in,resuspension |
| R5287 | T6378 | T6379 | compound | SDS,lysis |
| R5288 | T6379 | T6380 | compound | lysis,buffer |
| R5289 | T6380 | T6377 | pobj | buffer,in |
| R5290 | T6381 | T6376 | punct | (,resuspension |
| R5291 | T6382 | T6384 | compound | ChIP,Kit |
| R5292 | T6383 | T6384 | compound | Assay,Kit |
| R5293 | T6384 | T6376 | appos | Kit,resuspension |
| R5294 | T6385 | T6384 | punct | ",",Kit |
| R5295 | T6386 | T6384 | appos | Upstate,Kit |
| R5296 | T6387 | T6384 | punct | ),Kit |
| R5297 | T6388 | T6373 | punct | .,on |
| R5298 | T6389 | T6394 | prep | After,resuspended |
| R5299 | T6390 | T6389 | pobj | centrifugation,After |
| R5300 | T6391 | T6394 | punct | ",",resuspended |
| R5301 | T6392 | T6394 | nsubjpass | pellets,resuspended |
| R5302 | T6393 | T6394 | auxpass | were,resuspended |
| R5303 | T6394 | T6394 | ROOT | resuspended,resuspended |
| R5304 | T6395 | T6394 | prep | in,resuspended |
| R5305 | T6396 | T6398 | compound | MNase,buffer |
| R5306 | T6397 | T6398 | compound | reaction,buffer |
| R5307 | T6398 | T6395 | pobj | buffer,in |
| R5308 | T6399 | T6394 | punct | (,resuspended |
| R5309 | T6400 | T6403 | nummod | 10,pH |
| R5310 | T6401 | T6403 | compound | mM,pH |
| R5311 | T6402 | T6403 | compound | Tris-HCl,pH |
| R5312 | T6403 | T6403 | ROOT | pH,pH |
| R5313 | T6404 | T6403 | nummod | 7.5,pH |
| R5314 | T6405 | T6403 | punct | ",",pH |
| R5315 | T6406 | T6408 | nummod | 10,NaCl |
| R5316 | T6407 | T6408 | compound | mM,NaCl |
| R5317 | T6408 | T6403 | appos | NaCl,pH |
| R5318 | T6409 | T6403 | punct | ",",pH |
| R5319 | T6410 | T6412 | nummod | 3,MgCl2 |
| R5320 | T6411 | T6412 | compound | mM,MgCl2 |
| R5321 | T6412 | T6403 | appos | MgCl2,pH |
| R5322 | T6413 | T6412 | punct | ",",MgCl2 |
| R5323 | T6414 | T6416 | nummod | 1,CaCl2 |
| R5324 | T6415 | T6416 | compound | mM,CaCl2 |
| R5325 | T6416 | T6412 | appos | CaCl2,MgCl2 |
| R5326 | T6417 | T6416 | punct | ",",CaCl2 |
| R5327 | T6418 | T6419 | nummod | 4,% |
| R5328 | T6419 | T6420 | compound | %,NP-40 |
| R5329 | T6420 | T6416 | appos | NP-40,CaCl2 |
| R5330 | T6421 | T6416 | punct | ),CaCl2 |
| R5331 | T6422 | T6394 | punct | .,resuspended |
| R5332 | T6423 | T6424 | compound | DNA,digestion |
| R5333 | T6424 | T6426 | nsubjpass | digestion,performed |
| R5334 | T6425 | T6426 | auxpass | was,performed |
| R5335 | T6426 | T6426 | ROOT | performed,performed |
| R5336 | T6427 | T6426 | advcl | using,performed |
| R5337 | T6428 | T6431 | nummod | 50,Nuclease |
| R5338 | T6429 | T6431 | compound | U,Nuclease |
| R5339 | T6430 | T6431 | compound | Micrococcal,Nuclease |
| R5340 | T6431 | T6427 | dobj | Nuclease,using |
| R5341 | T6432 | T6433 | punct | (,Fermentas |
| R5342 | T6433 | T6431 | appos | Fermentas,Nuclease |
| R5343 | T6434 | T6433 | punct | ),Fermentas |
| R5344 | T6435 | T6431 | cc | and,Nuclease |
| R5345 | T6436 | T6439 | nummod | 1,cells |
| R5346 | T6437 | T6439 | nummod | ×,cells |
| R5347 | T6438 | T6439 | nummod | 107,cells |
| R5348 | T6439 | T6427 | dobj | cells,using |
| R5349 | T6440 | T6439 | prep | per,cells |
| R5350 | T6441 | T6440 | pobj | tube,per |
| R5351 | T6442 | T6427 | prep | in,using |
| R5352 | T6443 | T6444 | det | a,volume |
| R5353 | T6444 | T6442 | pobj | volume,in |
| R5354 | T6445 | T6444 | prep | of,volume |
| R5355 | T6446 | T6447 | nummod | 1.5,ml |
| R5356 | T6447 | T6445 | pobj | ml,of |
| R5357 | T6448 | T6426 | punct | .,performed |
| R5358 | T6449 | T6455 | prep | After,stopped |
| R5359 | T6450 | T6451 | nummod | 2,min |
| R5360 | T6451 | T6449 | pobj | min,After |
| R5361 | T6452 | T6455 | punct | ",",stopped |
| R5362 | T6453 | T6455 | nsubjpass | reaction,stopped |
| R5363 | T6454 | T6455 | auxpass | was,stopped |
| R5364 | T6455 | T6455 | ROOT | stopped,stopped |
| R5365 | T6456 | T6455 | prep | by,stopped |
| R5366 | T6457 | T6456 | pcomp | adding,by |
| R5367 | T6458 | T6460 | quantmod | 30,200 |
| R5368 | T6459 | T6460 | quantmod | µl,200 |
| R5369 | T6460 | T6457 | dobj | 200,adding |
| R5370 | T6461 | T6462 | compound | mM,EGTA |
| R5371 | T6462 | T6457 | dobj | EGTA,adding |
| R5372 | T6463 | T6455 | punct | .,stopped |
| R5373 | T6464 | T6465 | amod | Further,steps |
| R5374 | T6465 | T6467 | nsubjpass | steps,performed |
| R5375 | T6466 | T6467 | auxpass | were,performed |
| R5376 | T6467 | T6467 | ROOT | performed,performed |
| R5377 | T6468 | T6467 | advcl | using,performed |
| R5378 | T6469 | T6473 | det | the,Kit |
| R5379 | T6470 | T6473 | compound | Chromatin,Kit |
| R5380 | T6471 | T6473 | compound | Immunoprecipitation,Kit |
| R5381 | T6472 | T6473 | compound | Assay,Kit |
| R5382 | T6473 | T6468 | dobj | Kit,using |
| R5383 | T6474 | T6473 | punct | (,Kit |
| R5384 | T6475 | T6473 | appos | Upstate,Kit |
| R5385 | T6476 | T6473 | punct | ),Kit |
| R5386 | T6477 | T6467 | prep | according,performed |
| R5387 | T6478 | T6477 | prep | to,according |
| R5388 | T6479 | T6480 | det | the,manufacturer |
| R5389 | T6480 | T6482 | poss | manufacturer,instructions |
| R5390 | T6481 | T6480 | case | 's,manufacturer |
| R5391 | T6482 | T6478 | pobj | instructions,to |
| R5392 | T6483 | T6467 | punct | .,performed |
| R5393 | T6484 | T6487 | nummod | 1,cells |
| R5394 | T6485 | T6487 | nummod | ×,cells |
| R5395 | T6486 | T6487 | nummod | 107,cells |
| R5396 | T6487 | T6516 | nsubjpass | cells,used |
| R5397 | T6488 | T6487 | cc | and,cells |
| R5398 | T6489 | T6491 | nummod | 2,antibody |
| R5399 | T6490 | T6491 | compound | µg,antibody |
| R5400 | T6491 | T6487 | conj | antibody,cells |
| R5401 | T6492 | T6516 | punct | (,used |
| R5402 | T6493 | T6516 | nsubjpass | Sp1,used |
| R5403 | T6494 | T6495 | punct | (,Pep2 |
| R5404 | T6495 | T6493 | appos | Pep2,Sp1 |
| R5405 | T6496 | T6495 | punct | ),Pep2 |
| R5406 | T6497 | T6516 | nsubjpass | sc-59,used |
| R5407 | T6498 | T6497 | punct | ",",sc-59 |
| R5408 | T6499 | T6497 | conj | Sp3,sc-59 |
| R5409 | T6500 | T6499 | punct | (,Sp3 |
| R5410 | T6501 | T6499 | appos | D-20,Sp3 |
| R5411 | T6502 | T6499 | punct | ),Sp3 |
| R5412 | T6503 | T6497 | conj | sc-644,sc-59 |
| R5413 | T6504 | T6503 | cc | or,sc-644 |
| R5414 | T6505 | T6503 | conj | actin,sc-644 |
| R5415 | T6506 | T6507 | punct | (,H-196 |
| R5416 | T6507 | T6505 | appos | H-196,actin |
| R5417 | T6508 | T6505 | punct | ),actin |
| R5418 | T6509 | T6505 | conj | sc-7210,actin |
| R5419 | T6510 | T6509 | punct | ",",sc-7210 |
| R5420 | T6511 | T6513 | compound | Santa,Biotechnology |
| R5421 | T6512 | T6513 | compound | Cruz,Biotechnology |
| R5422 | T6513 | T6509 | appos | Biotechnology,sc-7210 |
| R5423 | T6514 | T6509 | punct | ),sc-7210 |
| R5424 | T6515 | T6516 | auxpass | were,used |
| R5425 | T6516 | T6516 | ROOT | used,used |
| R5426 | T6517 | T6516 | prep | for,used |
| R5427 | T6518 | T6519 | det | each,immunoprecipitation |
| R5428 | T6519 | T6517 | pobj | immunoprecipitation,for |
| R5429 | T6520 | T6516 | punct | .,used |
| R5430 | T6521 | T6522 | det | All,buffers |
| R5431 | T6522 | T6525 | nsubjpass | buffers,supplied |
| R5432 | T6523 | T6525 | auxpass | were,supplied |
| R5433 | T6524 | T6525 | advmod | freshly,supplied |
| R5434 | T6525 | T6525 | ROOT | supplied,supplied |
| R5435 | T6526 | T6525 | prep | with,supplied |
| R5436 | T6527 | T6528 | compound | protease,inhibitors |
| R5437 | T6528 | T6526 | pobj | inhibitors,with |
| R5438 | T6529 | T6525 | punct | .,supplied |
| R5439 | T6530 | T6539 | prep | After,resolved |
| R5440 | T6531 | T6532 | compound | phenol/chloroform,extraction |
| R5441 | T6532 | T6535 | nmod | extraction,precipitation |
| R5442 | T6533 | T6532 | cc | and,extraction |
| R5443 | T6534 | T6532 | conj | ethanol,extraction |
| R5444 | T6535 | T6530 | pobj | precipitation,After |
| R5445 | T6536 | T6539 | punct | ",",resolved |
| R5446 | T6537 | T6539 | nsubjpass | DNA,resolved |
| R5447 | T6538 | T6539 | auxpass | was,resolved |
| R5448 | T6539 | T6539 | ROOT | resolved,resolved |
| R5449 | T6540 | T6539 | prep | in,resolved |
| R5450 | T6541 | T6542 | nummod | 16,µl |
| R5451 | T6542 | T6540 | pobj | µl,in |
| R5452 | T6543 | T6542 | appos | H2O,µl |
| R5453 | T6544 | T6539 | punct | .,resolved |
| R5454 | T6545 | T6546 | compound | Immunoprecipitated,DNA |
| R5455 | T6546 | T6548 | nsubjpass | DNA,detected |
| R5456 | T6547 | T6548 | auxpass | was,detected |
| R5457 | T6548 | T6548 | ROOT | detected,detected |
| R5458 | T6549 | T6548 | agent | by,detected |
| R5459 | T6550 | T6551 | amod | nested,PCR |
| R5460 | T6551 | T6549 | pobj | PCR,by |
| R5461 | T6552 | T6548 | advcl | using,detected |
| R5462 | T6553 | T6554 | nummod | 4,µl |
| R5463 | T6554 | T6552 | dobj | µl,using |
| R5464 | T6555 | T6554 | prep | of,µl |
| R5465 | T6556 | T6558 | det | the,DNA |
| R5466 | T6557 | T6558 | amod | resolved,DNA |
| R5467 | T6558 | T6555 | pobj | DNA,of |
| R5468 | T6559 | T6558 | prep | in,DNA |
| R5469 | T6560 | T6562 | det | the,PCR |
| R5470 | T6561 | T6562 | amod | first,PCR |
| R5471 | T6562 | T6559 | pobj | PCR,in |
| R5472 | T6563 | T6548 | punct | ",",detected |
| R5473 | T6564 | T6548 | cc | and,detected |
| R5474 | T6565 | T6566 | nummod | 1,µl |
| R5475 | T6566 | T6548 | conj | µl,detected |
| R5476 | T6567 | T6566 | prep | for,µl |
| R5477 | T6568 | T6570 | det | the,PCR |
| R5478 | T6569 | T6570 | amod | nested,PCR |
| R5479 | T6570 | T6567 | pobj | PCR,for |
| R5480 | T6571 | T6548 | punct | .,detected |
| R5481 | T6572 | T6583 | prep | For,used |
| R5482 | T6573 | T6572 | pobj | amplification,For |
| R5483 | T6574 | T6573 | prep | of,amplification |
| R5484 | T6575 | T6577 | det | the,promoter |
| R5485 | T6576 | T6577 | compound | A3G,promoter |
| R5486 | T6577 | T6574 | pobj | promoter,of |
| R5487 | T6578 | T6583 | punct | ",",used |
| R5488 | T6579 | T6581 | det | the,primers |
| R5489 | T6580 | T6581 | amod | following,primers |
| R5490 | T6581 | T6583 | nsubjpass | primers,used |
| R5491 | T6582 | T6583 | auxpass | were,used |
| R5492 | T6583 | T6583 | ROOT | used,used |
| R5493 | T6584 | T6589 | punct | :,ccacggtggcctccgagggtga-3 |
| R5494 | T6585 | T6589 | ccomp | ChIP3Gplus,ccacggtggcctccgagggtga-3 |
| R5495 | T6586 | T6585 | nummod | 5,ChIP3Gplus |
| R5496 | T6587 | T6589 | punct | ′,ccacggtggcctccgagggtga-3 |
| R5497 | T6588 | T6589 | punct | -,ccacggtggcctccgagggtga-3 |
| R5498 | T6589 | T6590 | compound | ccacggtggcctccgagggtga-3,′ |
| R5499 | T6590 | T6583 | dobj | ′,used |
| R5500 | T6591 | T6590 | cc | and,′ |
| R5501 | T6592 | T6590 | conj | ChIP3Gminus,′ |
| R5502 | T6593 | T6594 | punct | :,5 |
| R5503 | T6594 | T6597 | dep | 5,ctctccaccatcaagacagac-3 |
| R5504 | T6595 | T6597 | punct | ′,ctctccaccatcaagacagac-3 |
| R5505 | T6596 | T6597 | punct | -,ctctccaccatcaagacagac-3 |
| R5506 | T6597 | T6598 | amod | ctctccaccatcaagacagac-3,′ |
| R5507 | T6598 | T6590 | appos | ′,′ |
| R5508 | T6599 | T6600 | punct | (,1 |
| R5509 | T6600 | T6598 | appos | 1,′ |
| R5510 | T6601 | T6583 | punct | .,used |
| R5511 | T6602 | T6602 | ROOT | PCR,PCR |
| R5512 | T6603 | T6602 | punct | ),PCR |
| R5513 | T6604 | T6614 | punct | ;,nested |
| R5514 | T6605 | T6614 | nsubj | ChIP3G2plus,nested |
| R5515 | T6606 | T6605 | punct | :,ChIP3G2plus |
| R5516 | T6607 | T6610 | dep | 5,tactctccctccctgtcccca-3 |
| R5517 | T6608 | T6610 | punct | ′,tactctccctccctgtcccca-3 |
| R5518 | T6609 | T6610 | punct | -,tactctccctccctgtcccca-3 |
| R5519 | T6610 | T6611 | compound | tactctccctccctgtcccca-3,′ |
| R5520 | T6611 | T6605 | appos | ′,ChIP3G2plus |
| R5521 | T6612 | T6611 | cc | and,′ |
| R5522 | T6613 | T6611 | conj | ChIP3G,′ |
| R5523 | T6614 | T6614 | ROOT | nested,nested |
| R5524 | T6615 | T6614 | cc | minus,nested |
| R5525 | T6616 | T6614 | punct | :,nested |
| R5526 | T6617 | T6621 | nummod | 5,′ |
| R5527 | T6618 | T6620 | punct | ′,aggctgatgcctccgcag-3 |
| R5528 | T6619 | T6620 | punct | -,aggctgatgcctccgcag-3 |
| R5529 | T6620 | T6621 | compound | aggctgatgcctccgcag-3,′ |
| R5530 | T6621 | T6623 | npadvmod | ′,nested |
| R5531 | T6622 | T6623 | punct | (,nested |
| R5532 | T6623 | T6623 | ROOT | nested,nested |
| R5533 | T6624 | T6623 | dobj | PCR,nested |
| R5534 | T6625 | T6623 | punct | ),nested |
| R5535 | T6626 | T6614 | punct | .,nested |
| R5536 | T6627 | T6628 | compound | Taq,polymerase |
| R5537 | T6628 | T6633 | nsubjpass | polymerase,used |
| R5538 | T6629 | T6630 | punct | (,Qiagen |
| R5539 | T6630 | T6628 | appos | Qiagen,polymerase |
| R5540 | T6631 | T6628 | punct | ),polymerase |
| R5541 | T6632 | T6633 | auxpass | was,used |
| R5542 | T6633 | T6633 | ROOT | used,used |
| R5543 | T6634 | T6635 | advmod | together,with |
| R5544 | T6635 | T6633 | prep | with,used |
| R5545 | T6636 | T6639 | det | the,conditions |
| R5546 | T6637 | T6639 | amod | following,conditions |
| R5547 | T6638 | T6639 | compound | cycle,conditions |
| R5548 | T6639 | T6635 | pobj | conditions,with |
| R5549 | T6640 | T6639 | punct | :,conditions |
| R5550 | T6641 | T6642 | nummod | one,cycle |
| R5551 | T6642 | T6639 | appos | cycle,conditions |
| R5552 | T6643 | T6644 | nummod | 94,° |
| R5553 | T6644 | T6645 | compound | °,C |
| R5554 | T6645 | T6642 | appos | C,cycle |
| R5555 | T6646 | T6645 | prep | for,C |
| R5556 | T6647 | T6648 | nummod | 2,min |
| R5557 | T6648 | T6646 | pobj | min,for |
| R5558 | T6649 | T6645 | punct | ;,C |
| R5559 | T6650 | T6651 | nummod | 30,cycles |
| R5560 | T6651 | T6642 | appos | cycles,cycle |
| R5561 | T6652 | T6653 | nummod | 94,° |
| R5562 | T6653 | T6654 | compound | °,C |
| R5563 | T6654 | T6642 | appos | C,cycle |
| R5564 | T6655 | T6639 | prep | for,conditions |
| R5565 | T6656 | T6662 | preconj | 30,for |
| R5566 | T6657 | T6656 | nmod | s,30 |
| R5567 | T6658 | T6657 | punct | ",",s |
| R5568 | T6659 | T6660 | nummod | 60,° |
| R5569 | T6660 | T6661 | compound | °,C |
| R5570 | T6661 | T6657 | appos | C,s |
| R5571 | T6662 | T6655 | prep | for,for |
| R5572 | T6663 | T6662 | pobj | 60,for |
| R5573 | T6664 | T6663 | nmod | s,60 |
| R5574 | T6665 | T6664 | punct | ",",s |
| R5575 | T6666 | T6668 | nummod | 72,C |
| R5576 | T6667 | T6668 | compound | °,C |
| R5577 | T6668 | T6664 | appos | C,s |
| R5578 | T6669 | T6663 | prep | for,60 |
| R5579 | T6670 | T6671 | nummod | 2,min |
| R5580 | T6671 | T6669 | pobj | min,for |
| R5581 | T6672 | T6677 | punct | ;,C |
| R5582 | T6673 | T6674 | nummod | one,cycle |
| R5583 | T6674 | T6677 | compound | cycle,C |
| R5584 | T6675 | T6676 | nummod | 72,° |
| R5585 | T6676 | T6677 | compound | °,C |
| R5586 | T6677 | T6663 | appos | C,60 |
| R5587 | T6678 | T6677 | prep | for,C |
| R5588 | T6679 | T6680 | nummod | 10,min |
| R5589 | T6680 | T6678 | pobj | min,for |
| R5590 | T6681 | T6633 | punct | .,used |
| R5591 | T6682 | T6694 | prep | As,targeted |
| R5592 | T6683 | T6685 | det | a,control |
| R5593 | T6684 | T6685 | amod | negative,control |
| R5594 | T6685 | T6682 | pobj | control,As |
| R5595 | T6686 | T6694 | punct | ",",targeted |
| R5596 | T6687 | T6688 | det | a,region |
| R5597 | T6688 | T6694 | nsubjpass | region,targeted |
| R5598 | T6689 | T6688 | prep | in,region |
| R5599 | T6690 | T6692 | det | the,gene |
| R5600 | T6691 | T6692 | compound | A3G,gene |
| R5601 | T6692 | T6689 | pobj | gene,in |
| R5602 | T6693 | T6694 | auxpass | was,targeted |
| R5603 | T6694 | T6694 | ROOT | targeted,targeted |
| R5604 | T6695 | T6694 | advcl | using,targeted |
| R5605 | T6696 | T6697 | det | the,primers |
| R5606 | T6697 | T6698 | compound | primers,ChIP3Gneg_plus |
| R5610 | T6701 | T6703 | punct | ′,taagtaccacccagagatgag-3 |
| R5611 | T6702 | T6703 | punct | -,taagtaccacccagagatgag-3 |
| R5612 | T6703 | T6704 | compound | taagtaccacccagagatgag-3,′ |
| R5613 | T6704 | T6698 | appos | ′,ChIP3Gneg_plus |
| R5614 | T6705 | T6704 | cc | and,′ |
| R5615 | T6706 | T6704 | conj | ChIP3Gneg_minus,′ |
| R5616 | T6707 | T6708 | punct | :,5 |
| R5617 | T6708 | T6712 | nummod | 5,′ |
| R5618 | T6709 | T6711 | punct | ′,catgatcttcatggtggcacg-3 |
| R5619 | T6710 | T6711 | punct | -,catgatcttcatggtggcacg-3 |
| R5620 | T6711 | T6712 | compound | catgatcttcatggtggcacg-3,′ |
| R5621 | T6712 | T6704 | appos | ′,′ |
| R5622 | T6713 | T6712 | prep | for,′ |
| R5623 | T6714 | T6716 | det | both,steps |
| R5624 | T6715 | T6716 | compound | PCR,steps |
| R5625 | T6716 | T6713 | pobj | steps,for |
| R5626 | T6717 | T6694 | punct | .,targeted |
| R5627 | T6718 | T6719 | compound | PCR,conditions |
| R5628 | T6719 | T6720 | nsubj | conditions,were |
| R5629 | T6720 | T6720 | ROOT | were,were |
| R5630 | T6721 | T6722 | det | the,same |
| R5631 | T6722 | T6720 | attr | same,were |
| R5632 | T6723 | T6722 | prep | as,same |
| R5633 | T6724 | T6723 | prep | for,as |
| R5634 | T6725 | T6728 | det | the,sequence |
| R5635 | T6726 | T6728 | compound | A3G,sequence |
| R5636 | T6727 | T6728 | compound | promoter,sequence |
| R5637 | T6728 | T6724 | pobj | sequence,for |
| R5638 | T6729 | T6720 | punct | ",",were |
| R5639 | T6730 | T6720 | prep | with,were |
| R5640 | T6731 | T6732 | det | the,exception |
| R5641 | T6732 | T6730 | pobj | exception,with |
| R5642 | T6733 | T6737 | mark | that,decreased |
| R5643 | T6734 | T6735 | amod | annealing,temperature |
| R5644 | T6735 | T6737 | nsubjpass | temperature,decreased |
| R5645 | T6736 | T6737 | auxpass | was,decreased |
| R5646 | T6737 | T6720 | advcl | decreased,were |
| R5647 | T6738 | T6737 | prep | to,decreased |
| R5648 | T6739 | T6741 | nummod | 55,C. |
| R5649 | T6740 | T6741 | nummod | °,C. |
| R5650 | T6741 | T6738 | pobj | C.,to |
| R5607 | T6698 | T6695 | dobj | ChIP3Gneg_plus,using |
| R5608 | T6699 | T6698 | punct | :,ChIP3Gneg_plus |
| R5609 | T6700 | T6703 | nummod | 5,taagtaccacccagagatgag-3 |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T5951 | 0-42 | Sentence | denotes | Chromatin immunoprecipitation (ChIP) assay |
| T5952 | 43-281 | Sentence | denotes | A3.01 cells were treated with RPMI culture medium containing 1% formaldehyde for 10 min. at 37°C. Cells were washed twice with ice-cold PBS and incubated for 10 min. on ice after resuspension in SDS lysis buffer (ChIP Assay Kit, Upstate). |
| T5953 | 282-424 | Sentence | denotes | After centrifugation, pellets were resuspended in MNase reaction buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 1 mM CaCl2, 4% NP-40). |
| T5954 | 425-546 | Sentence | denotes | DNA digestion was performed using 50 U Micrococcal Nuclease (Fermentas) and 1 × 107 cells per tube in a volume of 1.5 ml. |
| T5955 | 547-609 | Sentence | denotes | After 2 min, reaction was stopped by adding 30 µl 200 mM EGTA. |
| T5956 | 610-744 | Sentence | denotes | Further steps were performed using the Chromatin Immunoprecipitation Assay Kit (Upstate) according to the manufacturer's instructions. |
| T5957 | 745-902 | Sentence | denotes | 1 × 107 cells and 2 µg antibody (Sp1(Pep2) sc-59, Sp3(D-20) sc-644 or actin(H-196) sc-7210, Santa Cruz Biotechnology) were used for each immunoprecipitation. |
| T5958 | 903-962 | Sentence | denotes | All buffers were freshly supplied with protease inhibitors. |
| T5959 | 963-1055 | Sentence | denotes | After phenol/chloroform extraction and ethanol precipitation, DNA was resolved in 16 µl H2O. |
| T5960 | 1056-1183 | Sentence | denotes | Immunoprecipitated DNA was detected by nested PCR using 4 µl of the resolved DNA in the first PCR, and 1 µl for the nested PCR. |
| T5961 | 1184-1255 | Sentence | denotes | For amplification of the A3G promoter, the following primers were used: |
| T5962 | 1256-1312 | Sentence | denotes | ChIP3Gplus 5′-ccacggtggcctccgagggtga-3′ and ChIP3Gminus: |
| T5963 | 1313-1344 | Sentence | denotes | 5′-ctctccaccatcaagacagac-3′ (1. |
| T5964 | 1345-1363 | Sentence | denotes | PCR); ChIP3G2plus: |
| T5965 | 1364-1416 | Sentence | denotes | 5′-tactctccctccctgtcccca-3′ and ChIP3G nested minus: |
| T5966 | 1417-1455 | Sentence | denotes | 5′-aggctgatgcctccgcag-3′ (nested PCR). |
| T5967 | 1456-1643 | Sentence | denotes | Taq polymerase (Qiagen) was used together with the following cycle conditions: one cycle 94°C for 2 min; 30 cycles 94°C for 30 s, 60°C for 60 s, 72°C for 2 min; one cycle 72°C for 10 min. |
| T5968 | 1644-1738 | Sentence | denotes | As a negative control, a region in the A3G gene was targeted using the primers ChIP3Gneg_plus: |
| T5969 | 1739-1787 | Sentence | denotes | 5′-taagtaccacccagagatgag-3′ and ChIP3Gneg_minus: |
| T5970 | 1788-1835 | Sentence | denotes | 5′-catgatcttcatggtggcacg-3′ for both PCR steps. |
| T5971 | 1836-1967 | Sentence | denotes | PCR conditions were the same as for the A3G promoter sequence, with the exception that annealing temperature was decreased to 55°C. |
| T99 | 0-42 | Sentence | denotes | Chromatin immunoprecipitation (ChIP) assay |
| T100 | 43-140 | Sentence | denotes | A3.01 cells were treated with RPMI culture medium containing 1% formaldehyde for 10 min. at 37°C. |
| T101 | 141-281 | Sentence | denotes | Cells were washed twice with ice-cold PBS and incubated for 10 min. on ice after resuspension in SDS lysis buffer (ChIP Assay Kit, Upstate). |
| T102 | 282-424 | Sentence | denotes | After centrifugation, pellets were resuspended in MNase reaction buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 1 mM CaCl2, 4% NP-40). |
| T103 | 425-546 | Sentence | denotes | DNA digestion was performed using 50 U Micrococcal Nuclease (Fermentas) and 1 × 107 cells per tube in a volume of 1.5 ml. |
| T104 | 547-609 | Sentence | denotes | After 2 min, reaction was stopped by adding 30 µl 200 mM EGTA. |
| T105 | 610-744 | Sentence | denotes | Further steps were performed using the Chromatin Immunoprecipitation Assay Kit (Upstate) according to the manufacturer's instructions. |
| T106 | 745-902 | Sentence | denotes | 1 × 107 cells and 2 µg antibody (Sp1(Pep2) sc-59, Sp3(D-20) sc-644 or actin(H-196) sc-7210, Santa Cruz Biotechnology) were used for each immunoprecipitation. |
| T107 | 903-962 | Sentence | denotes | All buffers were freshly supplied with protease inhibitors. |
| T108 | 963-1055 | Sentence | denotes | After phenol/chloroform extraction and ethanol precipitation, DNA was resolved in 16 µl H2O. |
| T109 | 1056-1183 | Sentence | denotes | Immunoprecipitated DNA was detected by nested PCR using 4 µl of the resolved DNA in the first PCR, and 1 µl for the nested PCR. |
| T110 | 1184-1255 | Sentence | denotes | For amplification of the A3G promoter, the following primers were used: |
| T111 | 1256-1312 | Sentence | denotes | ChIP3Gplus 5′-ccacggtggcctccgagggtga-3′ and ChIP3Gminus: |
| T112 | 1313-1344 | Sentence | denotes | 5′-ctctccaccatcaagacagac-3′ (1. |
| T113 | 1345-1363 | Sentence | denotes | PCR); ChIP3G2plus: |
| T114 | 1364-1416 | Sentence | denotes | 5′-tactctccctccctgtcccca-3′ and ChIP3G nested minus: |
| T115 | 1417-1455 | Sentence | denotes | 5′-aggctgatgcctccgcag-3′ (nested PCR). |
| T116 | 1456-1643 | Sentence | denotes | Taq polymerase (Qiagen) was used together with the following cycle conditions: one cycle 94°C for 2 min; 30 cycles 94°C for 30 s, 60°C for 60 s, 72°C for 2 min; one cycle 72°C for 10 min. |
| T117 | 1644-1738 | Sentence | denotes | As a negative control, a region in the A3G gene was targeted using the primers ChIP3Gneg_plus: |
| T118 | 1739-1787 | Sentence | denotes | 5′-taagtaccacccagagatgag-3′ and ChIP3Gneg_minus: |
| T119 | 1788-1835 | Sentence | denotes | 5′-catgatcttcatggtggcacg-3′ for both PCR steps. |
| T120 | 1836-1967 | Sentence | denotes | PCR conditions were the same as for the A3G promoter sequence, with the exception that annealing temperature was decreased to 55°C. |
pmc-enju-pas
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T5974 | 0-9 | NN | denotes | Chromatin |
| T5975 | 10-29 | NN | denotes | immunoprecipitation |
| T5976 | 30-31 | -LRB- | denotes | ( |
| T5977 | 31-35 | NN | denotes | ChIP |
| T5978 | 35-36 | -RRB- | denotes | ) |
| T5979 | 37-42 | NN | denotes | assay |
| T5980 | 43-48 | NN | denotes | A3.01 |
| T5981 | 49-54 | NN | denotes | cells |
| T5982 | 55-59 | VB | denotes | were |
| T5983 | 60-67 | VB | denotes | treated |
| T5984 | 68-72 | IN | denotes | with |
| T5985 | 73-77 | NN | denotes | RPMI |
| T5986 | 78-85 | NN | denotes | culture |
| T5987 | 86-92 | NN | denotes | medium |
| T5988 | 93-103 | VB | denotes | containing |
| T5989 | 104-105 | CD | denotes | 1 |
| T5990 | 105-106 | NN | denotes | % |
| T5991 | 107-119 | NN | denotes | formaldehyde |
| T5992 | 120-123 | IN | denotes | for |
| T5993 | 124-131 | CD | denotes | 10 min. |
| T5994 | 132-134 | IN | denotes | at |
| T5995 | 135-140 | NNP | denotes | 37°C. |
| T5996 | 141-146 | NNP | denotes | Cells |
| T5997 | 147-151 | VB | denotes | were |
| T5998 | 152-158 | VB | denotes | washed |
| T5999 | 159-164 | RB | denotes | twice |
| T6000 | 165-169 | IN | denotes | with |
| T6001 | 170-178 | JJ | denotes | ice-cold |
| T6002 | 179-182 | NN | denotes | PBS |
| T6003 | 183-186 | CC | denotes | and |
| T6004 | 187-196 | VB | denotes | incubated |
| T6005 | 197-200 | IN | denotes | for |
| T6006 | 201-208 | CD | denotes | 10 min. |
| T6007 | 209-211 | IN | denotes | on |
| T6008 | 212-215 | NN | denotes | ice |
| T6009 | 216-221 | IN | denotes | after |
| T6010 | 222-234 | NN | denotes | resuspension |
| T6011 | 235-237 | IN | denotes | in |
| T6012 | 238-241 | NN | denotes | SDS |
| T6013 | 242-247 | NN | denotes | lysis |
| T6014 | 248-254 | NN | denotes | buffer |
| T6015 | 255-256 | -LRB- | denotes | ( |
| T6016 | 256-260 | NN | denotes | ChIP |
| T6017 | 261-266 | NNP | denotes | Assay |
| T6018 | 267-270 | NNP | denotes | Kit |
| T6019 | 270-271 | -COMMA- | denotes | , |
| T6020 | 272-279 | NNP | denotes | Upstate |
| T6021 | 279-280 | -RRB- | denotes | ) |
| T6022 | 282-287 | IN | denotes | After |
| T6023 | 288-302 | NN | denotes | centrifugation |
| T6024 | 302-303 | -COMMA- | denotes | , |
| T6025 | 304-311 | NN | denotes | pellets |
| T6026 | 312-316 | VB | denotes | were |
| T6027 | 317-328 | VB | denotes | resuspended |
| T6028 | 329-331 | IN | denotes | in |
| T6029 | 332-337 | NN | denotes | MNase |
| T6030 | 338-346 | NN | denotes | reaction |
| T6031 | 347-353 | NN | denotes | buffer |
| T6032 | 354-355 | -LRB- | denotes | ( |
| T6033 | 355-360 | NN | denotes | 10 mM |
| T6034 | 361-369 | NN | denotes | Tris-HCl |
| T6035 | 370-372 | NN | denotes | pH |
| T6036 | 373-376 | CD | denotes | 7.5 |
| T6037 | 376-377 | -COMMA- | denotes | , |
| T6038 | 378-383 | NN | denotes | 10 mM |
| T6039 | 384-388 | NN | denotes | NaCl |
| T6040 | 388-389 | -COMMA- | denotes | , |
| T6041 | 390-394 | NN | denotes | 3 mM |
| T6042 | 395-400 | NN | denotes | MgCl2 |
| T6043 | 400-401 | -COMMA- | denotes | , |
| T6044 | 402-406 | NN | denotes | 1 mM |
| T6045 | 407-412 | NN | denotes | CaCl2 |
| T6046 | 412-413 | -COMMA- | denotes | , |
| T6047 | 414-415 | CD | denotes | 4 |
| T6048 | 415-416 | NN | denotes | % |
| T6049 | 417-422 | NN | denotes | NP-40 |
| T6050 | 422-423 | -RRB- | denotes | ) |
| T6051 | 425-428 | NN | denotes | DNA |
| T6052 | 429-438 | NN | denotes | digestion |
| T6053 | 439-442 | VB | denotes | was |
| T6054 | 443-452 | VB | denotes | performed |
| T6055 | 453-458 | VB | denotes | using |
| T6056 | 459-461 | CD | denotes | 50 |
| T6057 | 462-463 | NNP | denotes | U |
| T6058 | 464-475 | NNP | denotes | Micrococcal |
| T6059 | 476-484 | NNP | denotes | Nuclease |
| T6060 | 485-486 | -LRB- | denotes | ( |
| T6061 | 486-495 | NN | denotes | Fermentas |
| T6062 | 495-496 | -RRB- | denotes | ) |
| T6063 | 497-500 | CC | denotes | and |
| T6064 | 501-508 | CD | denotes | 1 × 107 |
| T6065 | 509-514 | NN | denotes | cells |
| T6066 | 515-518 | IN | denotes | per |
| T6067 | 519-523 | NN | denotes | tube |
| T6068 | 524-526 | IN | denotes | in |
| T6069 | 527-528 | DT | denotes | a |
| T6070 | 529-535 | NN | denotes | volume |
| T6071 | 536-538 | IN | denotes | of |
| T6072 | 539-545 | NN | denotes | 1.5 ml |
| T6073 | 547-552 | IN | denotes | After |
| T6074 | 553-558 | NN | denotes | 2 min |
| T6075 | 558-559 | -COMMA- | denotes | , |
| T6076 | 560-568 | NN | denotes | reaction |
| T6077 | 569-572 | VB | denotes | was |
| T6078 | 573-580 | VB | denotes | stopped |
| T6079 | 581-583 | IN | denotes | by |
| T6080 | 584-590 | VB | denotes | adding |
| T6081 | 591-596 | JJ | denotes | 30 µl |
| T6082 | 597-603 | NN | denotes | 200 mM |
| T6083 | 604-608 | NN | denotes | EGTA |
| T6084 | 610-617 | JJ | denotes | Further |
| T6085 | 618-623 | NN | denotes | steps |
| T6086 | 624-628 | VB | denotes | were |
| T6087 | 629-638 | VB | denotes | performed |
| T6088 | 639-644 | VB | denotes | using |
| T6089 | 645-648 | DT | denotes | the |
| T6090 | 649-658 | NNP | denotes | Chromatin |
| T6091 | 659-678 | NNP | denotes | Immunoprecipitation |
| T6092 | 679-684 | NNP | denotes | Assay |
| T6093 | 685-688 | NN | denotes | Kit |
| T6094 | 689-690 | -LRB- | denotes | ( |
| T6095 | 690-697 | NN | denotes | Upstate |
| T6096 | 697-698 | -RRB- | denotes | ) |
| T6097 | 699-708 | VB | denotes | according |
| T6098 | 709-711 | TO | denotes | to |
| T6099 | 712-715 | DT | denotes | the |
| T6100 | 716-728 | NN | denotes | manufacturer |
| T6101 | 728-730 | POS | denotes | 's |
| T6102 | 731-743 | NN | denotes | instructions |
| T6103 | 745-752 | CD | denotes | 1 × 107 |
| T6104 | 753-758 | NN | denotes | cells |
| T6105 | 759-762 | CC | denotes | and |
| T6106 | 763-767 | NN | denotes | 2 µg |
| T6107 | 768-776 | NN | denotes | antibody |
| T6108 | 777-778 | -LRB- | denotes | ( |
| T6109 | 778-781 | NN | denotes | Sp1 |
| T6110 | 781-782 | -LRB- | denotes | ( |
| T6111 | 782-786 | NN | denotes | Pep2 |
| T6112 | 786-787 | -RRB- | denotes | ) |
| T6113 | 788-793 | NN | denotes | sc-59 |
| T6114 | 793-794 | -COMMA- | denotes | , |
| T6115 | 795-798 | NN | denotes | Sp3 |
| T6116 | 798-799 | -LRB- | denotes | ( |
| T6117 | 799-803 | NN | denotes | D-20 |
| T6118 | 803-804 | -RRB- | denotes | ) |
| T6119 | 805-811 | NN | denotes | sc-644 |
| T6120 | 812-814 | CC | denotes | or |
| T6121 | 815-820 | NN | denotes | actin |
| T6122 | 820-821 | -LRB- | denotes | ( |
| T6123 | 821-826 | NN | denotes | H-196 |
| T6124 | 826-827 | -RRB- | denotes | ) |
| T6125 | 828-835 | NN | denotes | sc-7210 |
| T6126 | 835-836 | -COMMA- | denotes | , |
| T6127 | 837-842 | NNP | denotes | Santa |
| T6128 | 843-847 | NNP | denotes | Cruz |
| T6129 | 848-861 | NNP | denotes | Biotechnology |
| T6130 | 861-862 | -RRB- | denotes | ) |
| T6131 | 863-867 | VB | denotes | were |
| T6132 | 868-872 | VB | denotes | used |
| T6133 | 873-876 | IN | denotes | for |
| T6134 | 877-881 | DT | denotes | each |
| T6135 | 882-901 | NN | denotes | immunoprecipitation |
| T6136 | 903-906 | DT | denotes | All |
| T6137 | 907-914 | NN | denotes | buffers |
| T6138 | 915-919 | VB | denotes | were |
| T6139 | 920-927 | RB | denotes | freshly |
| T6140 | 928-936 | VB | denotes | supplied |
| T6141 | 937-941 | IN | denotes | with |
| T6142 | 942-950 | NN | denotes | protease |
| T6143 | 951-961 | NN | denotes | inhibitors |
| T6144 | 963-968 | IN | denotes | After |
| T6145 | 969-986 | NN | denotes | phenol/chloroform |
| T6146 | 987-997 | NN | denotes | extraction |
| T6147 | 998-1001 | CC | denotes | and |
| T6148 | 1002-1009 | NN | denotes | ethanol |
| T6149 | 1010-1023 | NN | denotes | precipitation |
| T6150 | 1023-1024 | -COMMA- | denotes | , |
| T6151 | 1025-1028 | NN | denotes | DNA |
| T6152 | 1029-1032 | VB | denotes | was |
| T6153 | 1033-1041 | VB | denotes | resolved |
| T6154 | 1042-1044 | IN | denotes | in |
| T6155 | 1045-1050 | NN | denotes | 16 µl |
| T6156 | 1051-1054 | NN | denotes | H2O |
| T6157 | 1056-1074 | VB | denotes | Immunoprecipitated |
| T6158 | 1075-1078 | NN | denotes | DNA |
| T6159 | 1079-1082 | VB | denotes | was |
| T6160 | 1083-1091 | VB | denotes | detected |
| T6161 | 1092-1094 | IN | denotes | by |
| T6162 | 1095-1101 | VB | denotes | nested |
| T6163 | 1102-1105 | NN | denotes | PCR |
| T6164 | 1106-1111 | VB | denotes | using |
| T6165 | 1112-1116 | NN | denotes | 4 µl |
| T6166 | 1117-1119 | IN | denotes | of |
| T6167 | 1120-1123 | DT | denotes | the |
| T6168 | 1124-1132 | VB | denotes | resolved |
| T6169 | 1133-1136 | NN | denotes | DNA |
| T6170 | 1137-1139 | IN | denotes | in |
| T6171 | 1140-1143 | DT | denotes | the |
| T6172 | 1144-1149 | JJ | denotes | first |
| T6173 | 1150-1153 | NN | denotes | PCR |
| T6174 | 1153-1154 | -COMMA- | denotes | , |
| T6175 | 1155-1158 | CC | denotes | and |
| T6176 | 1159-1163 | NN | denotes | 1 µl |
| T6177 | 1164-1167 | IN | denotes | for |
| T6178 | 1168-1171 | DT | denotes | the |
| T6179 | 1172-1178 | JJ | denotes | nested |
| T6180 | 1179-1182 | NN | denotes | PCR |
| T6181 | 1184-1187 | IN | denotes | For |
| T6182 | 1188-1201 | NN | denotes | amplification |
| T6183 | 1202-1204 | IN | denotes | of |
| T6184 | 1205-1208 | DT | denotes | the |
| T6185 | 1209-1212 | NN | denotes | A3G |
| T6186 | 1213-1221 | NN | denotes | promoter |
| T6187 | 1221-1222 | -COMMA- | denotes | , |
| T6188 | 1223-1226 | DT | denotes | the |
| T6189 | 1227-1236 | VB | denotes | following |
| T6190 | 1237-1244 | NN | denotes | primers |
| T6191 | 1245-1249 | VB | denotes | were |
| T6192 | 1250-1254 | VB | denotes | used |
| T6193 | 1254-1255 | -COLON- | denotes | : |
| T6194 | 1256-1266 | NN | denotes | ChIP3Gplus |
| T6195 | 1267-1295 | CD | denotes | 5′-ccacggtggcctccgagggtga-3′ |
| T6196 | 1296-1299 | CC | denotes | and |
| T6197 | 1300-1311 | NN | denotes | ChIP3Gminus |
| T6198 | 1311-1312 | -COLON- | denotes | : |
| T6199 | 1313-1340 | CD | denotes | 5′-ctctccaccatcaagacagac-3′ |
| T6200 | 1341-1342 | -LRB- | denotes | ( |
| T6201 | 1342-1343 | CD | denotes | 1 |
| T6202 | 1345-1348 | NN | denotes | PCR |
| T6203 | 1348-1349 | -RRB- | denotes | ) |
| T6204 | 1349-1350 | -COLON- | denotes | ; |
| T6205 | 1351-1362 | NN | denotes | ChIP3G2plus |
| T6206 | 1362-1363 | -COLON- | denotes | : |
| T6207 | 1364-1391 | JJ | denotes | 5′-tactctccctccctgtcccca-3′ |
| T6208 | 1392-1395 | CC | denotes | and |
| T6209 | 1396-1402 | NN | denotes | ChIP3G |
| T6210 | 1403-1409 | VB | denotes | nested |
| T6211 | 1410-1415 | NN | denotes | minus |
| T6212 | 1415-1416 | -COLON- | denotes | : |
| T6213 | 1417-1441 | CD | denotes | 5′-aggctgatgcctccgcag-3′ |
| T6214 | 1442-1443 | -LRB- | denotes | ( |
| T6215 | 1443-1449 | VB | denotes | nested |
| T6216 | 1450-1453 | NN | denotes | PCR |
| T6217 | 1453-1454 | -RRB- | denotes | ) |
| T6218 | 1456-1459 | NN | denotes | Taq |
| T6219 | 1460-1470 | NN | denotes | polymerase |
| T6220 | 1471-1472 | -LRB- | denotes | ( |
| T6221 | 1472-1478 | NN | denotes | Qiagen |
| T6222 | 1478-1479 | -RRB- | denotes | ) |
| T6223 | 1480-1483 | VB | denotes | was |
| T6224 | 1484-1488 | VB | denotes | used |
| T6225 | 1489-1497 | RB | denotes | together |
| T6226 | 1498-1502 | IN | denotes | with |
| T6227 | 1503-1506 | DT | denotes | the |
| T6228 | 1507-1516 | VB | denotes | following |
| T6229 | 1517-1522 | NN | denotes | cycle |
| T6230 | 1523-1533 | NN | denotes | conditions |
| T6231 | 1533-1534 | -COLON- | denotes | : |
| T6232 | 1535-1538 | CD | denotes | one |
| T6233 | 1539-1544 | NN | denotes | cycle |
| T6234 | 1545-1549 | NN | denotes | 94°C |
| T6235 | 1550-1553 | IN | denotes | for |
| T6236 | 1554-1559 | NN | denotes | 2 min |
| T6237 | 1559-1560 | -COLON- | denotes | ; |
| T6238 | 1561-1563 | CD | denotes | 30 |
| T6239 | 1564-1570 | NN | denotes | cycles |
| T6240 | 1571-1575 | NN | denotes | 94°C |
| T6241 | 1576-1579 | IN | denotes | for |
| T6242 | 1580-1584 | NN | denotes | 30 s |
| T6243 | 1584-1585 | -COMMA- | denotes | , |
| T6244 | 1586-1590 | NN | denotes | 60°C |
| T6245 | 1591-1594 | IN | denotes | for |
| T6246 | 1595-1599 | NN | denotes | 60 s |
| T6247 | 1599-1600 | -COMMA- | denotes | , |
| T6248 | 1601-1605 | NN | denotes | 72°C |
| T6249 | 1606-1609 | IN | denotes | for |
| T6250 | 1610-1615 | NN | denotes | 2 min |
| T6251 | 1615-1616 | -COLON- | denotes | ; |
| T6252 | 1617-1620 | CD | denotes | one |
| T6253 | 1621-1626 | NN | denotes | cycle |
| T6254 | 1627-1631 | NN | denotes | 72°C |
| T6255 | 1632-1635 | IN | denotes | for |
| T6256 | 1636-1642 | NN | denotes | 10 min |
| T6257 | 1644-1646 | IN | denotes | As |
| T6258 | 1647-1648 | DT | denotes | a |
| T6259 | 1649-1657 | JJ | denotes | negative |
| T6260 | 1658-1665 | NN | denotes | control |
| T6261 | 1665-1666 | -COMMA- | denotes | , |
| T6262 | 1667-1668 | DT | denotes | a |
| T6263 | 1669-1675 | NN | denotes | region |
| T6264 | 1676-1678 | IN | denotes | in |
| T6265 | 1679-1682 | DT | denotes | the |
| T6266 | 1683-1686 | NN | denotes | A3G |
| T6267 | 1687-1691 | NN | denotes | gene |
| T6268 | 1692-1695 | VB | denotes | was |
| T6269 | 1696-1704 | VB | denotes | targeted |
| T6270 | 1705-1710 | VB | denotes | using |
| T6271 | 1711-1714 | DT | denotes | the |
| T6272 | 1715-1722 | NN | denotes | primers |
| T6273 | 1723-1737 | NN | denotes | ChIP3Gneg_plus |
| T6274 | 1737-1738 | -COLON- | denotes | : |
| T6275 | 1739-1766 | JJ | denotes | 5′-taagtaccacccagagatgag-3′ |
| T6276 | 1767-1770 | CC | denotes | and |
| T6277 | 1771-1786 | NN | denotes | ChIP3Gneg_minus |
| T6278 | 1786-1787 | -COLON- | denotes | : |
| T6279 | 1788-1815 | NN | denotes | 5′-catgatcttcatggtggcacg-3′ |
| T6280 | 1816-1819 | IN | denotes | for |
| T6281 | 1820-1824 | DT | denotes | both |
| T6282 | 1825-1828 | NN | denotes | PCR |
| T6283 | 1829-1834 | NN | denotes | steps |
| T6284 | 1836-1839 | NN | denotes | PCR |
| T6285 | 1840-1850 | NN | denotes | conditions |
| T6286 | 1851-1855 | VB | denotes | were |
| T6287 | 1856-1859 | DT | denotes | the |
| T6288 | 1860-1864 | JJ | denotes | same |
| T6289 | 1865-1867 | IN | denotes | as |
| T6290 | 1868-1871 | IN | denotes | for |
| T6291 | 1872-1875 | DT | denotes | the |
| T6292 | 1876-1879 | NN | denotes | A3G |
| T6293 | 1880-1888 | NN | denotes | promoter |
| T6294 | 1889-1897 | NN | denotes | sequence |
| T6295 | 1897-1898 | -COMMA- | denotes | , |
| T6296 | 1899-1903 | IN | denotes | with |
| T6297 | 1904-1907 | DT | denotes | the |
| T6298 | 1908-1917 | NN | denotes | exception |
| T6299 | 1918-1922 | IN | denotes | that |
| T6300 | 1923-1932 | VB | denotes | annealing |
| T6301 | 1933-1944 | NN | denotes | temperature |
| T6302 | 1945-1948 | VB | denotes | was |
| T6303 | 1949-1958 | VB | denotes | decreased |
| T6304 | 1959-1961 | TO | denotes | to |
| T6305 | 1962-1966 | NN | denotes | 55°C |
| R4915 | T5975 | T5974 | arg1Of | immunoprecipitation,Chromatin |
| R4916 | T5975 | T5976 | arg1Of | immunoprecipitation,( |
| R4917 | T5977 | T5976 | arg2Of | ChIP,( |
| R4918 | T5978 | T5976 | arg3Of | ),( |
| R4919 | T5979 | T5975 | arg1Of | assay,immunoprecipitation |
| R4920 | T5981 | T5980 | arg1Of | cells,A3.01 |
| R4921 | T5981 | T5982 | arg1Of | cells,were |
| R4922 | T5981 | T5983 | arg2Of | cells,treated |
| R4923 | T5983 | T5982 | arg2Of | treated,were |
| R4924 | T5983 | T5984 | arg1Of | treated,with |
| R4925 | T5985 | T5984 | arg2Of | RPMI,with |
| R4926 | T5987 | T5986 | arg1Of | medium,culture |
| R4927 | T5987 | T5988 | arg1Of | medium,containing |
| R4928 | T5987 | T5994 | arg1Of | medium,at |
| R4929 | T5987 | T5997 | arg1Of | medium,were |
| R4930 | T5987 | T5998 | arg2Of | medium,washed |
| R4931 | T5987 | T6004 | arg2Of | medium,incubated |
| R4932 | T5989 | T5990 | arg1Of | 1,% |
| R4933 | T5991 | T5988 | arg2Of | formaldehyde,containing |
| R4934 | T5991 | T5989 | arg1Of | formaldehyde,1 |
| R4935 | T5991 | T5992 | arg1Of | formaldehyde,for |
| R4936 | T5993 | T5992 | arg2Of | 10 min.,for |
| R4937 | T5996 | T5994 | arg2Of | Cells,at |
| R4938 | T5996 | T5995 | arg1Of | Cells,37°C. |
| R4939 | T5998 | T5999 | arg1Of | washed,twice |
| R4940 | T5998 | T6000 | arg1Of | washed,with |
| R4941 | T5998 | T6003 | arg1Of | washed,and |
| R4942 | T6002 | T6000 | arg2Of | PBS,with |
| R4943 | T6002 | T6001 | arg1Of | PBS,ice-cold |
| R4944 | T6003 | T5982 | modOf | and,were |
| R4945 | T6003 | T5997 | arg2Of | and,were |
| R4946 | T6004 | T6003 | arg2Of | incubated,and |
| R4947 | T6004 | T6005 | arg1Of | incubated,for |
| R4948 | T6006 | T6005 | arg2Of | 10 min.,for |
| R4949 | T6006 | T6007 | arg1Of | 10 min.,on |
| R4950 | T6008 | T6007 | arg2Of | ice,on |
| R4951 | T6008 | T6009 | arg1Of | ice,after |
| R4952 | T6010 | T6009 | arg2Of | resuspension,after |
| R4953 | T6010 | T6011 | arg1Of | resuspension,in |
| R4954 | T6014 | T6011 | arg2Of | buffer,in |
| R4955 | T6014 | T6012 | arg1Of | buffer,SDS |
| R4956 | T6014 | T6013 | arg1Of | buffer,lysis |
| R4957 | T6014 | T6015 | arg1Of | buffer,( |
| R4958 | T6018 | T6015 | arg2Of | Kit,( |
| R4959 | T6018 | T6016 | arg1Of | Kit,ChIP |
| R4960 | T6018 | T6017 | arg1Of | Kit,Assay |
| R4961 | T6018 | T6019 | arg1Of | Kit,"," |
| R4962 | T6020 | T6019 | arg2Of | Upstate,"," |
| R4963 | T6021 | T6015 | arg3Of | ),( |
| R4964 | T6023 | T6022 | arg2Of | centrifugation,After |
| R4965 | T6025 | T6026 | arg1Of | pellets,were |
| R4966 | T6025 | T6027 | arg2Of | pellets,resuspended |
| R4967 | T6027 | T6022 | arg1Of | resuspended,After |
| R4968 | T6027 | T6024 | arg1Of | resuspended,"," |
| R4969 | T6027 | T6026 | arg2Of | resuspended,were |
| R4970 | T6027 | T6028 | arg1Of | resuspended,in |
| R4971 | T6031 | T6028 | arg2Of | buffer,in |
| R4972 | T6031 | T6029 | arg1Of | buffer,MNase |
| R4973 | T6031 | T6030 | arg1Of | buffer,reaction |
| R4974 | T6031 | T6032 | arg1Of | buffer,( |
| R4975 | T6035 | T6033 | arg1Of | pH,10 mM |
| R4976 | T6035 | T6034 | arg1Of | pH,Tris-HCl |
| R4977 | T6035 | T6036 | arg1Of | pH,7.5 |
| R4978 | T6035 | T6037 | arg1Of | pH,"," |
| R4979 | T6037 | T6040 | arg1Of | ",","," |
| R4980 | T6039 | T6037 | arg2Of | NaCl,"," |
| R4981 | T6039 | T6038 | arg1Of | NaCl,10 mM |
| R4982 | T6040 | T6043 | arg1Of | ",","," |
| R4983 | T6042 | T6040 | arg2Of | MgCl2,"," |
| R4984 | T6042 | T6041 | arg1Of | MgCl2,3 mM |
| R4985 | T6043 | T6046 | arg1Of | ",","," |
| R4986 | T6045 | T6043 | arg2Of | CaCl2,"," |
| R4987 | T6045 | T6044 | arg1Of | CaCl2,1 mM |
| R4988 | T6046 | T6032 | arg2Of | ",",( |
| R4989 | T6047 | T6048 | arg1Of | 4,% |
| R4990 | T6049 | T6046 | arg2Of | NP-40,"," |
| R4991 | T6049 | T6047 | arg1Of | NP-40,4 |
| R4992 | T6050 | T6032 | arg3Of | ),( |
| R4993 | T6052 | T6051 | arg1Of | digestion,DNA |
| R4994 | T6052 | T6053 | arg1Of | digestion,was |
| R4995 | T6052 | T6054 | arg2Of | digestion,performed |
| R4996 | T6054 | T6053 | arg2Of | performed,was |
| R4997 | T6055 | T6054 | arg3Of | using,performed |
| R4998 | T6055 | T6068 | arg1Of | using,in |
| R4999 | T6059 | T6056 | arg1Of | Nuclease,50 |
| R5000 | T6059 | T6057 | arg1Of | Nuclease,U |
| R5001 | T6059 | T6058 | arg1Of | Nuclease,Micrococcal |
| R5002 | T6059 | T6060 | arg1Of | Nuclease,( |
| R5003 | T6059 | T6063 | arg1Of | Nuclease,and |
| R5004 | T6061 | T6060 | arg2Of | Fermentas,( |
| R5005 | T6062 | T6060 | arg3Of | ),( |
| R5006 | T6063 | T6055 | arg2Of | and,using |
| R5007 | T6065 | T6063 | arg2Of | cells,and |
| R5008 | T6065 | T6064 | arg1Of | cells,1 × 107 |
| R5009 | T6065 | T6066 | arg1Of | cells,per |
| R5010 | T6067 | T6066 | arg2Of | tube,per |
| R5011 | T6070 | T6068 | arg2Of | volume,in |
| R5012 | T6070 | T6069 | arg1Of | volume,a |
| R5013 | T6070 | T6071 | arg1Of | volume,of |
| R5014 | T6072 | T6071 | arg2Of | 1.5 ml,of |
| R5015 | T6074 | T6073 | arg2Of | 2 min,After |
| R5016 | T6076 | T6077 | arg1Of | reaction,was |
| R5017 | T6076 | T6078 | arg2Of | reaction,stopped |
| R5018 | T6078 | T6073 | arg1Of | stopped,After |
| R5019 | T6078 | T6075 | arg1Of | stopped,"," |
| R5020 | T6078 | T6077 | arg2Of | stopped,was |
| R5021 | T6078 | T6079 | arg1Of | stopped,by |
| R5022 | T6080 | T6079 | arg2Of | adding,by |
| R5023 | T6083 | T6080 | arg2Of | EGTA,adding |
| R5024 | T6083 | T6081 | arg1Of | EGTA,30 µl |
| R5025 | T6083 | T6082 | arg1Of | EGTA,200 mM |
| R5026 | T6085 | T6084 | arg1Of | steps,Further |
| R5027 | T6085 | T6086 | arg1Of | steps,were |
| R5028 | T6085 | T6087 | arg2Of | steps,performed |
| R5029 | T6087 | T6086 | arg2Of | performed,were |
| R5030 | T6088 | T6087 | arg3Of | using,performed |
| R5031 | T6088 | T6097 | arg1Of | using,according |
| R5032 | T6092 | T6090 | arg1Of | Assay,Chromatin |
| R5033 | T6092 | T6091 | arg1Of | Assay,Immunoprecipitation |
| R5034 | T6093 | T6088 | arg2Of | Kit,using |
| R5035 | T6093 | T6089 | arg1Of | Kit,the |
| R5036 | T6093 | T6092 | arg1Of | Kit,Assay |
| R5037 | T6093 | T6094 | arg1Of | Kit,( |
| R5038 | T6095 | T6094 | arg2Of | Upstate,( |
| R5039 | T6096 | T6094 | arg3Of | ),( |
| R5040 | T6098 | T6097 | arg2Of | to,according |
| R5041 | T6100 | T6099 | arg1Of | manufacturer,the |
| R5042 | T6100 | T6101 | arg2Of | manufacturer,'s |
| R5043 | T6102 | T6098 | arg2Of | instructions,to |
| R5044 | T6102 | T6101 | arg1Of | instructions,'s |
| R5045 | T6104 | T6103 | arg1Of | cells,1 × 107 |
| R5046 | T6104 | T6105 | arg1Of | cells,and |
| R5047 | T6105 | T6131 | arg1Of | and,were |
| R5048 | T6105 | T6132 | arg2Of | and,used |
| R5049 | T6107 | T6105 | arg2Of | antibody,and |
| R5050 | T6107 | T6106 | arg1Of | antibody,2 µg |
| R5051 | T6107 | T6108 | arg1Of | antibody,( |
| R5052 | T6111 | T6110 | arg2Of | Pep2,( |
| R5053 | T6112 | T6110 | arg3Of | ),( |
| R5054 | T6113 | T6109 | arg1Of | sc-59,Sp1 |
| R5055 | T6113 | T6110 | arg1Of | sc-59,( |
| R5056 | T6113 | T6114 | arg1Of | sc-59,"," |
| R5057 | T6114 | T6120 | arg1Of | ",",or |
| R5058 | T6117 | T6116 | arg2Of | D-20,( |
| R5059 | T6118 | T6116 | arg3Of | ),( |
| R5060 | T6119 | T6114 | arg2Of | sc-644,"," |
| R5061 | T6119 | T6115 | arg1Of | sc-644,Sp3 |
| R5062 | T6119 | T6116 | arg1Of | sc-644,( |
| R5063 | T6120 | T6108 | arg2Of | or,( |
| R5064 | T6123 | T6122 | arg2Of | H-196,( |
| R5065 | T6124 | T6122 | arg3Of | ),( |
| R5066 | T6125 | T6120 | arg2Of | sc-7210,or |
| R5067 | T6125 | T6121 | arg1Of | sc-7210,actin |
| R5068 | T6125 | T6122 | arg1Of | sc-7210,( |
| R5069 | T6125 | T6126 | arg1Of | sc-7210,"," |
| R5070 | T6129 | T6126 | arg2Of | Biotechnology,"," |
| R5071 | T6129 | T6127 | arg1Of | Biotechnology,Santa |
| R5072 | T6129 | T6128 | arg1Of | Biotechnology,Cruz |
| R5073 | T6130 | T6108 | arg3Of | ),( |
| R5074 | T6132 | T6131 | arg2Of | used,were |
| R5075 | T6132 | T6133 | arg1Of | used,for |
| R5076 | T6135 | T6133 | arg2Of | immunoprecipitation,for |
| R5077 | T6135 | T6134 | arg1Of | immunoprecipitation,each |
| R5078 | T6137 | T6136 | arg1Of | buffers,All |
| R5079 | T6137 | T6138 | arg1Of | buffers,were |
| R5080 | T6137 | T6140 | arg2Of | buffers,supplied |
| R5081 | T6140 | T6138 | arg2Of | supplied,were |
| R5082 | T6140 | T6139 | arg1Of | supplied,freshly |
| R5083 | T6140 | T6141 | arg1Of | supplied,with |
| R5084 | T6143 | T6141 | arg2Of | inhibitors,with |
| R5085 | T6143 | T6142 | arg1Of | inhibitors,protease |
| R5086 | T6146 | T6145 | arg1Of | extraction,phenol/chloroform |
| R5087 | T6146 | T6147 | arg1Of | extraction,and |
| R5088 | T6147 | T6144 | arg2Of | and,After |
| R5089 | T6149 | T6147 | arg2Of | precipitation,and |
| R5090 | T6149 | T6148 | arg1Of | precipitation,ethanol |
| R5091 | T6151 | T6152 | arg1Of | DNA,was |
| R5092 | T6151 | T6153 | arg2Of | DNA,resolved |
| R5093 | T6153 | T6144 | arg1Of | resolved,After |
| R5094 | T6153 | T6150 | arg1Of | resolved,"," |
| R5095 | T6153 | T6152 | arg2Of | resolved,was |
| R5096 | T6153 | T6154 | arg1Of | resolved,in |
| R5097 | T6156 | T6154 | arg2Of | H2O,in |
| R5098 | T6156 | T6155 | arg1Of | H2O,16 µl |
| R5099 | T6158 | T6157 | arg2Of | DNA,Immunoprecipitated |
| R5100 | T6158 | T6159 | arg1Of | DNA,was |
| R5101 | T6158 | T6160 | arg2Of | DNA,detected |
| R5102 | T6158 | T6164 | arg1Of | DNA,using |
| R5103 | T6160 | T6159 | arg2Of | detected,was |
| R5104 | T6160 | T6164 | modOf | detected,using |
| R5105 | T6163 | T6160 | arg1Of | PCR,detected |
| R5106 | T6163 | T6161 | arg2Of | PCR,by |
| R5107 | T6163 | T6162 | arg2Of | PCR,nested |
| R5108 | T6165 | T6164 | arg2Of | 4 µl,using |
| R5109 | T6165 | T6166 | arg1Of | 4 µl,of |
| R5110 | T6169 | T6168 | arg2Of | DNA,resolved |
| R5111 | T6169 | T6170 | arg1Of | DNA,in |
| R5112 | T6169 | T6175 | arg1Of | DNA,and |
| R5113 | T6173 | T6170 | arg2Of | PCR,in |
| R5114 | T6173 | T6171 | arg1Of | PCR,the |
| R5115 | T6173 | T6172 | arg1Of | PCR,first |
| R5116 | T6175 | T6166 | arg2Of | and,of |
| R5117 | T6175 | T6167 | arg1Of | and,the |
| R5118 | T6175 | T6174 | arg1Of | and,"," |
| R5119 | T6176 | T6175 | arg2Of | 1 µl,and |
| R5120 | T6176 | T6177 | arg1Of | 1 µl,for |
| R5121 | T6180 | T6177 | arg2Of | PCR,for |
| R5122 | T6180 | T6178 | arg1Of | PCR,the |
| R5123 | T6180 | T6179 | arg1Of | PCR,nested |
| R5124 | T6182 | T6181 | arg2Of | amplification,For |
| R5125 | T6182 | T6183 | arg1Of | amplification,of |
| R5126 | T6186 | T6183 | arg2Of | promoter,of |
| R5127 | T6186 | T6184 | arg1Of | promoter,the |
| R5128 | T6186 | T6185 | arg1Of | promoter,A3G |
| R5129 | T6190 | T6188 | arg1Of | primers,the |
| R5130 | T6190 | T6189 | arg1Of | primers,following |
| R5131 | T6190 | T6191 | arg1Of | primers,were |
| R5132 | T6190 | T6192 | arg2Of | primers,used |
| R5133 | T6192 | T6181 | arg1Of | used,For |
| R5134 | T6192 | T6187 | arg1Of | used,"," |
| R5135 | T6192 | T6191 | arg2Of | used,were |
| R5136 | T6192 | T6193 | arg1Of | used,: |
| R5137 | T6194 | T6195 | arg1Of | ChIP3Gplus,5′-ccacggtggcctccgagggtga-3′ |
| R5138 | T6194 | T6196 | arg1Of | ChIP3Gplus,and |
| R5139 | T6197 | T6196 | arg2Of | ChIP3Gminus,and |
| R5140 | T6197 | T6198 | arg1Of | ChIP3Gminus,: |
| R5141 | T6199 | T6200 | arg1Of | 5′-ctctccaccatcaagacagac-3′,( |
| R5142 | T6199 | T6201 | arg1Of | 5′-ctctccaccatcaagacagac-3′,1 |
| R5143 | T6202 | T6203 | arg1Of | PCR,) |
| R5144 | T6202 | T6204 | arg1Of | PCR,; |
| R5145 | T6205 | T6204 | arg2Of | ChIP3G2plus,; |
| R5146 | T6205 | T6206 | arg1Of | ChIP3G2plus,: |
| R5147 | T6207 | T6208 | arg1Of | 5′-tactctccctccctgtcccca-3′,and |
| R5148 | T6208 | T6212 | arg1Of | and,: |
| R5149 | T6210 | T6209 | arg1Of | nested,ChIP3G |
| R5150 | T6211 | T6208 | arg2Of | minus,and |
| R5151 | T6211 | T6210 | arg1Of | minus,nested |
| R5152 | T6213 | T6214 | arg1Of | 5′-aggctgatgcctccgcag-3′,( |
| R5153 | T6215 | T6214 | arg2Of | nested,( |
| R5154 | T6216 | T6215 | arg3Of | PCR,nested |
| R5155 | T6217 | T6214 | arg3Of | ),( |
| R5156 | T6219 | T6218 | arg1Of | polymerase,Taq |
| R5157 | T6219 | T6220 | arg1Of | polymerase,( |
| R5158 | T6219 | T6223 | arg1Of | polymerase,was |
| R5159 | T6219 | T6224 | arg2Of | polymerase,used |
| R5160 | T6221 | T6220 | arg2Of | Qiagen,( |
| R5161 | T6222 | T6220 | arg3Of | ),( |
| R5162 | T6224 | T6223 | arg2Of | used,was |
| R5163 | T6224 | T6225 | arg1Of | used,together |
| R5164 | T6224 | T6226 | arg1Of | used,with |
| R5165 | T6230 | T6226 | arg2Of | conditions,with |
| R5166 | T6230 | T6227 | arg1Of | conditions,the |
| R5167 | T6230 | T6228 | arg1Of | conditions,following |
| R5168 | T6230 | T6229 | arg1Of | conditions,cycle |
| R5169 | T6230 | T6231 | arg1Of | conditions,: |
| R5170 | T6234 | T6231 | arg2Of | 94°C,: |
| R5171 | T6234 | T6232 | arg1Of | 94°C,one |
| R5172 | T6234 | T6233 | arg1Of | 94°C,cycle |
| R5173 | T6234 | T6235 | arg1Of | 94°C,for |
| R5174 | T6234 | T6237 | arg1Of | 94°C,; |
| R5175 | T6236 | T6235 | arg2Of | 2 min,for |
| R5176 | T6239 | T6238 | arg1Of | cycles,30 |
| R5177 | T6240 | T6241 | arg1Of | 94°C,for |
| R5178 | T6240 | T6249 | arg1Of | 94°C,for |
| R5179 | T6240 | T6251 | arg1Of | 94°C,; |
| R5180 | T6242 | T6241 | arg2Of | 30 s,for |
| R5181 | T6242 | T6243 | arg1Of | 30 s,"," |
| R5182 | T6242 | T6247 | arg1Of | 30 s,"," |
| R5183 | T6244 | T6243 | arg2Of | 60°C,"," |
| R5184 | T6244 | T6245 | arg1Of | 60°C,for |
| R5185 | T6246 | T6245 | arg2Of | 60 s,for |
| R5186 | T6248 | T6247 | arg2Of | 72°C,"," |
| R5187 | T6250 | T6249 | arg2Of | 2 min,for |
| R5188 | T6251 | T6237 | arg2Of | ;,; |
| R5189 | T6251 | T6239 | arg1Of | ;,cycles |
| R5190 | T6254 | T6251 | arg2Of | 72°C,; |
| R5191 | T6254 | T6252 | arg1Of | 72°C,one |
| R5192 | T6254 | T6253 | arg1Of | 72°C,cycle |
| R5193 | T6254 | T6255 | arg1Of | 72°C,for |
| R5194 | T6256 | T6255 | arg2Of | 10 min,for |
| R5195 | T6260 | T6257 | arg2Of | control,As |
| R5196 | T6260 | T6258 | arg1Of | control,a |
| R5197 | T6260 | T6259 | arg1Of | control,negative |
| R5198 | T6263 | T6262 | arg1Of | region,a |
| R5199 | T6263 | T6264 | arg1Of | region,in |
| R5200 | T6263 | T6268 | arg1Of | region,was |
| R5201 | T6263 | T6269 | arg2Of | region,targeted |
| R5202 | T6267 | T6264 | arg2Of | gene,in |
| R5203 | T6267 | T6265 | arg1Of | gene,the |
| R5204 | T6267 | T6266 | arg1Of | gene,A3G |
| R5205 | T6269 | T6257 | arg1Of | targeted,As |
| R5206 | T6269 | T6261 | arg1Of | targeted,"," |
| R5207 | T6269 | T6268 | arg2Of | targeted,was |
| R5208 | T6269 | T6270 | modOf | targeted,using |
| R5209 | T6269 | T6274 | arg1Of | targeted,: |
| R5210 | T6273 | T6270 | arg2Of | ChIP3Gneg_plus,using |
| R5211 | T6273 | T6271 | arg1Of | ChIP3Gneg_plus,the |
| R5212 | T6273 | T6272 | arg1Of | ChIP3Gneg_plus,primers |
| R5213 | T6275 | T6276 | arg1Of | 5′-taagtaccacccagagatgag-3′,and |
| R5214 | T6276 | T6278 | arg1Of | and,: |
| R5215 | T6277 | T6276 | arg2Of | ChIP3Gneg_minus,and |
| R5216 | T6279 | T6280 | arg1Of | 5′-catgatcttcatggtggcacg-3′,for |
| R5217 | T6283 | T6280 | arg2Of | steps,for |
| R5218 | T6283 | T6281 | arg1Of | steps,both |
| R5219 | T6283 | T6282 | arg1Of | steps,PCR |
| R5220 | T6285 | T6284 | arg1Of | conditions,PCR |
| R5221 | T6285 | T6286 | arg1Of | conditions,were |
| R5222 | T6286 | T6295 | arg1Of | were,"," |
| R5223 | T6286 | T6296 | arg1Of | were,with |
| R5224 | T6288 | T6286 | arg2Of | same,were |
| R5225 | T6288 | T6287 | arg1Of | same,the |
| R5226 | T6288 | T6289 | arg1Of | same,as |
| R5227 | T6289 | T6290 | arg1Of | as,for |
| R5228 | T6294 | T6290 | arg2Of | sequence,for |
| R5229 | T6294 | T6291 | arg1Of | sequence,the |
| R5230 | T6294 | T6292 | arg1Of | sequence,A3G |
| R5231 | T6294 | T6293 | arg1Of | sequence,promoter |
| R5232 | T6298 | T6296 | arg2Of | exception,with |
| R5233 | T6298 | T6297 | arg1Of | exception,the |
| R5234 | T6298 | T6299 | arg1Of | exception,that |
| R5235 | T6301 | T6300 | arg1Of | temperature,annealing |
| R5236 | T6301 | T6302 | arg1Of | temperature,was |
| R5237 | T6301 | T6303 | arg2Of | temperature,decreased |
| R5238 | T6303 | T6299 | arg2Of | decreased,that |
| R5239 | T6303 | T6302 | arg2Of | decreased,was |
| R5240 | T6303 | T6304 | arg1Of | decreased,to |
| R5241 | T6305 | T6304 | arg2Of | 55°C,to |
events-check-again
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T6790 | 464-484 | Protein | denotes | Micrococcal Nuclease |
| T6791 | 778-781 | Protein | denotes | Sp1 |
| T6792 | 795-798 | Protein | denotes | Sp3 |
| T6793 | 815-820 | Protein | denotes | actin |
| T6794 | 1209-1212 | Protein | denotes | A3G |
| T6795 | 1456-1470 | Protein | denotes | Taq polymerase |
| T6796 | 1683-1686 | Protein | denotes | A3G |
| T6797 | 1876-1879 | Protein | denotes | A3G |
bionlp-st-ge-2016-reference-tees
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T6798 | 0-9 | Protein | denotes | Chromatin |
| T6799 | 10-29 | Binding | denotes | immunoprecipitation |
| T6800 | 332-337 | Protein | denotes | MNase |
| T6801 | 649-658 | Protein | denotes | Chromatin |
| T6802 | 763-776 | Protein | denotes | 2 µg antibody |
| T6803 | 778-781 | Protein | denotes | Sp1 |
| T6804 | 782-786 | Protein | denotes | Pep2 |
| T6805 | 795-798 | Protein | denotes | Sp3 |
| T6806 | 799-803 | Protein | denotes | D-20 |
| T6807 | 815-820 | Protein | denotes | actin |
| T6808 | 1209-1221 | Protein | denotes | A3G promoter |
| T6809 | 1456-1470 | Protein | denotes | Taq polymerase |
| T6810 | 1683-1691 | Protein | denotes | A3G gene |
| T6811 | 1876-1897 | Protein | denotes | A3G promoter sequence |
| R5651 | T6798 | T6799 | themeOf | Chromatin,immunoprecipitation |
bionlp-st-ge-2016-reference
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T5943 | 464-484 | Protein | denotes | Micrococcal Nuclease |
| T5944 | 778-781 | Protein | denotes | Sp1 |
| T5945 | 795-798 | Protein | denotes | Sp3 |
| T5946 | 815-820 | Protein | denotes | actin |
| T5947 | 1209-1212 | Protein | denotes | A3G |
| T5948 | 1456-1470 | Protein | denotes | Taq polymerase |
| T5949 | 1683-1686 | Protein | denotes | A3G |
| T5950 | 1876-1879 | Protein | denotes | A3G |
bionlp-st-ge-2016-uniprot
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T6306 | 370-372 | P0A7Z4 | denotes | pH |
| T6307 | 778-781 | P08047 | denotes | Sp1 |
| T6308 | 795-798 | Q02447 | denotes | Sp3 |
| T6309 | 1209-1212 | Q9HC16 | denotes | A3G |
| T6310 | 1683-1686 | Q9HC16 | denotes | A3G |
| T6311 | 1876-1879 | Q9HC16 | denotes | A3G |