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PMC:1920263 / 15340-17307 JSONTXT

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test2

Id Subject Object Predicate Lexical cue
T5934 464-484 Protein denotes Micrococcal Nuclease
T5935 778-781 Protein denotes Sp1
T5936 795-798 Protein denotes Sp3
T5937 815-820 Protein denotes actin
T5938 1209-1212 Protein denotes A3G
T5939 1456-1470 Protein denotes Taq polymerase
T5940 1683-1686 Protein denotes A3G
T5941 1876-1879 Protein denotes A3G

GO-CC

Id Subject Object Predicate Lexical cue
T6743 0-9 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T6744 649-658 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T6745 49-54 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6746 509-514 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6747 753-758 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6748 768-776 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6749 768-776 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

GO-MF

Id Subject Object Predicate Lexical cue
T6742 768-776 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-BP

Id Subject Object Predicate Lexical cue
T5972 242-247 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T5973 429-438 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion

UBERON-AE

Id Subject Object Predicate Lexical cue
T5942 519-523 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T6333 0-9 NNP denotes Chromatin
T6334 10-29 NN denotes immunoprecipitation
T6335 30-31 -LRB- denotes (
T6336 31-35 NN denotes ChIP
T6337 35-36 -RRB- denotes )
T6338 37-42 NN denotes assay
T6339 43-45 CD denotes A3
T6340 45-48 CD denotes .01
T6341 49-54 NNS denotes cells
T6342 55-59 VBD denotes were
T6343 60-67 VBN denotes treated
T6344 68-72 IN denotes with
T6345 73-77 NNP denotes RPMI
T6346 78-85 NN denotes culture
T6347 86-92 NN denotes medium
T6348 93-103 VBG denotes containing
T6349 104-105 CD denotes 1
T6350 105-106 NN denotes %
T6351 107-119 NN denotes formaldehyde
T6352 120-123 IN denotes for
T6353 124-126 CD denotes 10
T6354 127-130 NN denotes min
T6355 130-131 . denotes .
T6356 132-134 IN denotes at
T6357 135-137 CD denotes 37
T6358 137-138 NN denotes °
T6359 138-140 NNP denotes C.
T6360 141-146 NNP denotes Cells
T6361 147-151 VBD denotes were
T6362 152-158 VBN denotes washed
T6363 159-164 RB denotes twice
T6364 165-169 IN denotes with
T6365 170-178 JJ denotes ice-cold
T6366 179-182 NNP denotes PBS
T6367 183-186 CC denotes and
T6368 187-196 VBN denotes incubated
T6369 197-200 IN denotes for
T6370 201-203 CD denotes 10
T6371 204-207 NN denotes min
T6372 207-208 . denotes .
T6373 209-211 IN denotes on
T6374 212-215 NN denotes ice
T6375 216-221 IN denotes after
T6376 222-234 NN denotes resuspension
T6377 235-237 IN denotes in
T6378 238-241 NNP denotes SDS
T6379 242-247 VBZ denotes lysis
T6380 248-254 NN denotes buffer
T6381 255-256 -LRB- denotes (
T6382 256-260 NNP denotes ChIP
T6383 261-266 NNP denotes Assay
T6384 267-270 NNP denotes Kit
T6385 270-271 , denotes ,
T6386 272-279 NNP denotes Upstate
T6387 279-280 -RRB- denotes )
T6388 280-281 . denotes .
T6389 282-287 IN denotes After
T6390 288-302 NN denotes centrifugation
T6391 302-303 , denotes ,
T6392 304-311 NNS denotes pellets
T6393 312-316 VBD denotes were
T6394 317-328 VBN denotes resuspended
T6395 329-331 IN denotes in
T6396 332-337 NNP denotes MNase
T6397 338-346 NN denotes reaction
T6398 347-353 NN denotes buffer
T6399 354-355 -LRB- denotes (
T6400 355-357 CD denotes 10
T6401 358-360 NNP denotes mM
T6402 361-369 NNP denotes Tris-HCl
T6403 370-372 NNP denotes pH
T6404 373-376 CD denotes 7.5
T6405 376-377 , denotes ,
T6406 378-380 CD denotes 10
T6407 381-383 NNP denotes mM
T6408 384-388 NNP denotes NaCl
T6409 388-389 , denotes ,
T6410 390-391 CD denotes 3
T6411 392-394 NNP denotes mM
T6412 395-400 NNP denotes MgCl2
T6413 400-401 , denotes ,
T6414 402-403 CD denotes 1
T6415 404-406 NNP denotes mM
T6416 407-412 NNP denotes CaCl2
T6417 412-413 , denotes ,
T6418 414-415 CD denotes 4
T6419 415-416 NN denotes %
T6420 417-422 NN denotes NP-40
T6421 422-423 -RRB- denotes )
T6422 423-424 . denotes .
T6423 425-428 NNP denotes DNA
T6424 429-438 NN denotes digestion
T6425 439-442 VBD denotes was
T6426 443-452 VBN denotes performed
T6427 453-458 VBG denotes using
T6428 459-461 CD denotes 50
T6429 462-463 NNP denotes U
T6430 464-475 NNP denotes Micrococcal
T6431 476-484 NNP denotes Nuclease
T6432 485-486 -LRB- denotes (
T6433 486-495 NNP denotes Fermentas
T6434 495-496 -RRB- denotes )
T6435 497-500 CC denotes and
T6436 501-502 CD denotes 1
T6437 503-504 CD denotes ×
T6438 505-508 CD denotes 107
T6439 509-514 NNS denotes cells
T6440 515-518 IN denotes per
T6441 519-523 NN denotes tube
T6442 524-526 IN denotes in
T6443 527-528 DT denotes a
T6444 529-535 NN denotes volume
T6445 536-538 IN denotes of
T6446 539-542 CD denotes 1.5
T6447 543-545 NN denotes ml
T6448 545-546 . denotes .
T6449 547-552 IN denotes After
T6450 553-554 CD denotes 2
T6451 555-558 NN denotes min
T6452 558-559 , denotes ,
T6453 560-568 NN denotes reaction
T6454 569-572 VBD denotes was
T6455 573-580 VBN denotes stopped
T6456 581-583 IN denotes by
T6457 584-590 VBG denotes adding
T6458 591-593 CD denotes 30
T6459 594-596 NN denotes µl
T6460 597-600 CD denotes 200
T6461 601-603 NNP denotes mM
T6462 604-608 NNP denotes EGTA
T6463 608-609 . denotes .
T6464 610-617 JJ denotes Further
T6465 618-623 NNS denotes steps
T6466 624-628 VBD denotes were
T6467 629-638 VBN denotes performed
T6468 639-644 VBG denotes using
T6469 645-648 DT denotes the
T6470 649-658 NNP denotes Chromatin
T6471 659-678 NNP denotes Immunoprecipitation
T6472 679-684 NNP denotes Assay
T6473 685-688 NNP denotes Kit
T6474 689-690 -LRB- denotes (
T6475 690-697 NNP denotes Upstate
T6476 697-698 -RRB- denotes )
T6477 699-708 VBG denotes according
T6478 709-711 TO denotes to
T6479 712-715 DT denotes the
T6480 716-728 NN denotes manufacturer
T6481 728-730 POS denotes 's
T6482 731-743 NNS denotes instructions
T6483 743-744 . denotes .
T6484 745-746 CD denotes 1
T6485 747-748 CD denotes ×
T6486 749-752 CD denotes 107
T6487 753-758 NNS denotes cells
T6488 759-762 CC denotes and
T6489 763-764 CD denotes 2
T6490 765-767 NN denotes µg
T6491 768-776 NN denotes antibody
T6492 777-778 -LRB- denotes (
T6493 778-781 NNP denotes Sp1
T6494 781-782 -LRB- denotes (
T6495 782-786 NNP denotes Pep2
T6496 786-787 -RRB- denotes )
T6497 788-793 JJ denotes sc-59
T6498 793-794 , denotes ,
T6499 795-798 JJ denotes Sp3
T6500 798-799 -LRB- denotes (
T6501 799-803 NN denotes D-20
T6502 803-804 -RRB- denotes )
T6503 805-811 JJ denotes sc-644
T6504 812-814 CC denotes or
T6505 815-820 NN denotes actin
T6506 820-821 -LRB- denotes (
T6507 821-826 NNP denotes H-196
T6508 826-827 -RRB- denotes )
T6509 828-835 JJ denotes sc-7210
T6510 835-836 , denotes ,
T6511 837-842 NNP denotes Santa
T6512 843-847 NNP denotes Cruz
T6513 848-861 NNP denotes Biotechnology
T6514 861-862 -RRB- denotes )
T6515 863-867 VBD denotes were
T6516 868-872 VBN denotes used
T6517 873-876 IN denotes for
T6518 877-881 DT denotes each
T6519 882-901 NN denotes immunoprecipitation
T6520 901-902 . denotes .
T6521 903-906 DT denotes All
T6522 907-914 NNS denotes buffers
T6523 915-919 VBD denotes were
T6524 920-927 RB denotes freshly
T6525 928-936 VBN denotes supplied
T6526 937-941 IN denotes with
T6527 942-950 NN denotes protease
T6528 951-961 NNS denotes inhibitors
T6529 961-962 . denotes .
T6530 963-968 IN denotes After
T6531 969-986 NN denotes phenol/chloroform
T6532 987-997 NN denotes extraction
T6533 998-1001 CC denotes and
T6534 1002-1009 NN denotes ethanol
T6535 1010-1023 NN denotes precipitation
T6536 1023-1024 , denotes ,
T6537 1025-1028 NNP denotes DNA
T6538 1029-1032 VBD denotes was
T6539 1033-1041 VBN denotes resolved
T6540 1042-1044 IN denotes in
T6541 1045-1047 CD denotes 16
T6542 1048-1050 NN denotes µl
T6543 1051-1054 CD denotes H2O
T6544 1054-1055 . denotes .
T6545 1056-1074 NNP denotes Immunoprecipitated
T6546 1075-1078 NNP denotes DNA
T6547 1079-1082 VBD denotes was
T6548 1083-1091 VBN denotes detected
T6549 1092-1094 IN denotes by
T6550 1095-1101 JJ denotes nested
T6551 1102-1105 NNP denotes PCR
T6552 1106-1111 VBG denotes using
T6553 1112-1113 CD denotes 4
T6554 1114-1116 NN denotes µl
T6555 1117-1119 IN denotes of
T6556 1120-1123 DT denotes the
T6557 1124-1132 VBN denotes resolved
T6558 1133-1136 NN denotes DNA
T6559 1137-1139 IN denotes in
T6560 1140-1143 DT denotes the
T6561 1144-1149 JJ denotes first
T6562 1150-1153 NNP denotes PCR
T6563 1153-1154 , denotes ,
T6564 1155-1158 CC denotes and
T6565 1159-1160 CD denotes 1
T6566 1161-1163 NN denotes µl
T6567 1164-1167 IN denotes for
T6568 1168-1171 DT denotes the
T6569 1172-1178 JJ denotes nested
T6570 1179-1182 NNP denotes PCR
T6571 1182-1183 . denotes .
T6572 1184-1187 IN denotes For
T6573 1188-1201 NN denotes amplification
T6574 1202-1204 IN denotes of
T6575 1205-1208 DT denotes the
T6576 1209-1212 NNP denotes A3G
T6577 1213-1221 NN denotes promoter
T6578 1221-1222 , denotes ,
T6579 1223-1226 DT denotes the
T6580 1227-1236 VBG denotes following
T6581 1237-1244 NNS denotes primers
T6582 1245-1249 VBD denotes were
T6583 1250-1254 VBN denotes used
T6584 1254-1255 : denotes :
T6585 1256-1266 JJ denotes ChIP3Gplus
T6586 1267-1268 CD denotes 5
T6587 1268-1269 SYM denotes
T6588 1269-1270 : denotes -
T6589 1270-1294 JJ denotes ccacggtggcctccgagggtga-3
T6590 1294-1295 NN denotes
T6591 1296-1299 CC denotes and
T6592 1300-1311 NNS denotes ChIP3Gminus
T6593 1311-1312 : denotes :
T6594 1313-1314 CD denotes 5
T6595 1314-1315 SYM denotes
T6596 1315-1316 : denotes -
T6597 1316-1339 JJ denotes ctctccaccatcaagacagac-3
T6598 1339-1340 NN denotes
T6599 1341-1342 -LRB- denotes (
T6600 1342-1343 CD denotes 1
T6601 1343-1344 . denotes .
T6602 1345-1348 NNP denotes PCR
T6603 1348-1349 -RRB- denotes )
T6604 1349-1350 : denotes ;
T6605 1351-1362 NNS denotes ChIP3G2plus
T6606 1362-1363 : denotes :
T6607 1364-1365 CD denotes 5
T6608 1365-1366 SYM denotes
T6609 1366-1367 : denotes -
T6610 1367-1390 JJ denotes tactctccctccctgtcccca-3
T6611 1390-1391 NN denotes
T6612 1392-1395 CC denotes and
T6613 1396-1402 NNP denotes ChIP3G
T6614 1403-1409 VBD denotes nested
T6615 1410-1415 CC denotes minus
T6616 1415-1416 : denotes :
T6617 1417-1418 CD denotes 5
T6618 1418-1419 SYM denotes
T6619 1419-1420 : denotes -
T6620 1420-1440 JJ denotes aggctgatgcctccgcag-3
T6621 1440-1441 NN denotes
T6622 1442-1443 -LRB- denotes (
T6623 1443-1449 JJ denotes nested
T6624 1450-1453 NNP denotes PCR
T6625 1453-1454 -RRB- denotes )
T6626 1454-1455 . denotes .
T6627 1456-1459 NNP denotes Taq
T6628 1460-1470 NN denotes polymerase
T6629 1471-1472 -LRB- denotes (
T6630 1472-1478 NNP denotes Qiagen
T6631 1478-1479 -RRB- denotes )
T6632 1480-1483 VBD denotes was
T6633 1484-1488 VBN denotes used
T6634 1489-1497 RB denotes together
T6635 1498-1502 IN denotes with
T6636 1503-1506 DT denotes the
T6637 1507-1516 JJ denotes following
T6638 1517-1522 NN denotes cycle
T6639 1523-1533 NNS denotes conditions
T6640 1533-1534 : denotes :
T6641 1535-1538 CD denotes one
T6642 1539-1544 NN denotes cycle
T6643 1545-1547 CD denotes 94
T6644 1547-1548 NN denotes °
T6645 1548-1549 NNP denotes C
T6646 1550-1553 IN denotes for
T6647 1554-1555 CD denotes 2
T6648 1556-1559 NN denotes min
T6649 1559-1560 : denotes ;
T6650 1561-1563 CD denotes 30
T6651 1564-1570 NNS denotes cycles
T6652 1571-1573 CD denotes 94
T6653 1573-1574 NN denotes °
T6654 1574-1575 NNP denotes C
T6655 1576-1579 IN denotes for
T6656 1580-1582 CD denotes 30
T6657 1583-1584 PRP denotes s
T6658 1584-1585 , denotes ,
T6659 1586-1588 CD denotes 60
T6660 1588-1589 NN denotes °
T6661 1589-1590 NNP denotes C
T6662 1591-1594 IN denotes for
T6663 1595-1597 CD denotes 60
T6664 1598-1599 PRP denotes s
T6665 1599-1600 , denotes ,
T6666 1601-1603 CD denotes 72
T6667 1603-1604 NN denotes °
T6668 1604-1605 NNP denotes C
T6669 1606-1609 IN denotes for
T6670 1610-1611 CD denotes 2
T6671 1612-1615 NN denotes min
T6672 1615-1616 : denotes ;
T6673 1617-1620 CD denotes one
T6674 1621-1626 NN denotes cycle
T6675 1627-1629 CD denotes 72
T6676 1629-1630 NN denotes °
T6677 1630-1631 NNP denotes C
T6678 1632-1635 IN denotes for
T6679 1636-1638 CD denotes 10
T6680 1639-1642 NN denotes min
T6681 1642-1643 . denotes .
T6682 1644-1646 IN denotes As
T6683 1647-1648 DT denotes a
T6684 1649-1657 JJ denotes negative
T6685 1658-1665 NN denotes control
T6686 1665-1666 , denotes ,
T6687 1667-1668 DT denotes a
T6688 1669-1675 NN denotes region
T6689 1676-1678 IN denotes in
T6690 1679-1682 DT denotes the
T6691 1683-1686 NNP denotes A3G
T6692 1687-1691 NN denotes gene
T6693 1692-1695 VBD denotes was
T6694 1696-1704 VBN denotes targeted
T6695 1705-1710 VBG denotes using
T6696 1711-1714 DT denotes the
T6697 1715-1722 NNS denotes primers
T6698 1723-1737 NNS denotes ChIP3Gneg_plus
T6699 1737-1738 : denotes :
T6700 1739-1740 CD denotes 5
T6701 1740-1741 SYM denotes
T6702 1741-1742 : denotes -
T6703 1742-1765 JJ denotes taagtaccacccagagatgag-3
T6704 1765-1766 NN denotes
T6705 1767-1770 CC denotes and
T6706 1771-1786 NNS denotes ChIP3Gneg_minus
T6707 1786-1787 : denotes :
T6708 1788-1789 CD denotes 5
T6709 1789-1790 SYM denotes
T6710 1790-1791 : denotes -
T6711 1791-1814 JJ denotes catgatcttcatggtggcacg-3
T6712 1814-1815 NN denotes
T6713 1816-1819 IN denotes for
T6714 1820-1824 DT denotes both
T6715 1825-1828 NNP denotes PCR
T6716 1829-1834 NNS denotes steps
T6717 1834-1835 . denotes .
T6718 1836-1839 NNP denotes PCR
T6719 1840-1850 NNS denotes conditions
T6720 1851-1855 VBD denotes were
T6721 1856-1859 DT denotes the
T6722 1860-1864 JJ denotes same
T6723 1865-1867 IN denotes as
T6724 1868-1871 IN denotes for
T6725 1872-1875 DT denotes the
T6726 1876-1879 NNP denotes A3G
T6727 1880-1888 NN denotes promoter
T6728 1889-1897 NN denotes sequence
T6729 1897-1898 , denotes ,
T6730 1899-1903 IN denotes with
T6731 1904-1907 DT denotes the
T6732 1908-1917 NN denotes exception
T6733 1918-1922 WDT denotes that
T6734 1923-1932 VBG denotes annealing
T6735 1933-1944 NN denotes temperature
T6736 1945-1948 VBD denotes was
T6737 1949-1958 VBN denotes decreased
T6738 1959-1961 TO denotes to
T6739 1962-1964 CD denotes 55
T6740 1964-1965 CD denotes °
T6741 1965-1967 NNP denotes C.
R5242 T6333 T6334 compound Chromatin,immunoprecipitation
R5243 T6334 T6334 ROOT immunoprecipitation,immunoprecipitation
R5244 T6335 T6336 punct (,ChIP
R5245 T6336 T6334 appos ChIP,immunoprecipitation
R5246 T6337 T6336 punct ),ChIP
R5247 T6338 T6343 nsubjpass assay,treated
R5248 T6339 T6338 nummod A3,assay
R5249 T6340 T6341 nummod .01,cells
R5250 T6341 T6343 nsubjpass cells,treated
R5251 T6342 T6343 auxpass were,treated
R5252 T6343 T6343 ROOT treated,treated
R5253 T6344 T6343 prep with,treated
R5254 T6345 T6347 compound RPMI,medium
R5255 T6346 T6347 compound culture,medium
R5256 T6347 T6344 pobj medium,with
R5257 T6348 T6344 pcomp containing,with
R5258 T6349 T6350 nummod 1,%
R5259 T6350 T6351 compound %,formaldehyde
R5260 T6351 T6348 dobj formaldehyde,containing
R5261 T6352 T6348 prep for,containing
R5262 T6353 T6354 nummod 10,min
R5263 T6354 T6352 pobj min,for
R5264 T6355 T6343 punct .,treated
R5265 T6356 T6362 prep at,washed
R5266 T6357 T6360 nummod 37,Cells
R5267 T6358 T6360 compound °,Cells
R5268 T6359 T6360 compound C.,Cells
R5269 T6360 T6362 nsubjpass Cells,washed
R5270 T6361 T6362 auxpass were,washed
R5271 T6362 T6362 ROOT washed,washed
R5272 T6363 T6362 advmod twice,washed
R5273 T6364 T6362 prep with,washed
R5274 T6365 T6366 amod ice-cold,PBS
R5275 T6366 T6364 pobj PBS,with
R5276 T6367 T6362 cc and,washed
R5277 T6368 T6362 conj incubated,washed
R5278 T6369 T6368 prep for,incubated
R5279 T6370 T6371 nummod 10,min
R5280 T6371 T6369 pobj min,for
R5281 T6372 T6362 punct .,washed
R5282 T6373 T6373 ROOT on,on
R5283 T6374 T6373 pobj ice,on
R5284 T6375 T6374 prep after,ice
R5285 T6376 T6375 pobj resuspension,after
R5286 T6377 T6376 prep in,resuspension
R5287 T6378 T6379 compound SDS,lysis
R5288 T6379 T6380 compound lysis,buffer
R5289 T6380 T6377 pobj buffer,in
R5290 T6381 T6376 punct (,resuspension
R5291 T6382 T6384 compound ChIP,Kit
R5292 T6383 T6384 compound Assay,Kit
R5293 T6384 T6376 appos Kit,resuspension
R5294 T6385 T6384 punct ",",Kit
R5295 T6386 T6384 appos Upstate,Kit
R5296 T6387 T6384 punct ),Kit
R5297 T6388 T6373 punct .,on
R5298 T6389 T6394 prep After,resuspended
R5299 T6390 T6389 pobj centrifugation,After
R5300 T6391 T6394 punct ",",resuspended
R5301 T6392 T6394 nsubjpass pellets,resuspended
R5302 T6393 T6394 auxpass were,resuspended
R5303 T6394 T6394 ROOT resuspended,resuspended
R5304 T6395 T6394 prep in,resuspended
R5305 T6396 T6398 compound MNase,buffer
R5306 T6397 T6398 compound reaction,buffer
R5307 T6398 T6395 pobj buffer,in
R5308 T6399 T6394 punct (,resuspended
R5309 T6400 T6403 nummod 10,pH
R5310 T6401 T6403 compound mM,pH
R5311 T6402 T6403 compound Tris-HCl,pH
R5312 T6403 T6403 ROOT pH,pH
R5313 T6404 T6403 nummod 7.5,pH
R5314 T6405 T6403 punct ",",pH
R5315 T6406 T6408 nummod 10,NaCl
R5316 T6407 T6408 compound mM,NaCl
R5317 T6408 T6403 appos NaCl,pH
R5318 T6409 T6403 punct ",",pH
R5319 T6410 T6412 nummod 3,MgCl2
R5320 T6411 T6412 compound mM,MgCl2
R5321 T6412 T6403 appos MgCl2,pH
R5322 T6413 T6412 punct ",",MgCl2
R5323 T6414 T6416 nummod 1,CaCl2
R5324 T6415 T6416 compound mM,CaCl2
R5325 T6416 T6412 appos CaCl2,MgCl2
R5326 T6417 T6416 punct ",",CaCl2
R5327 T6418 T6419 nummod 4,%
R5328 T6419 T6420 compound %,NP-40
R5329 T6420 T6416 appos NP-40,CaCl2
R5330 T6421 T6416 punct ),CaCl2
R5331 T6422 T6394 punct .,resuspended
R5332 T6423 T6424 compound DNA,digestion
R5333 T6424 T6426 nsubjpass digestion,performed
R5334 T6425 T6426 auxpass was,performed
R5335 T6426 T6426 ROOT performed,performed
R5336 T6427 T6426 advcl using,performed
R5337 T6428 T6431 nummod 50,Nuclease
R5338 T6429 T6431 compound U,Nuclease
R5339 T6430 T6431 compound Micrococcal,Nuclease
R5340 T6431 T6427 dobj Nuclease,using
R5341 T6432 T6433 punct (,Fermentas
R5342 T6433 T6431 appos Fermentas,Nuclease
R5343 T6434 T6433 punct ),Fermentas
R5344 T6435 T6431 cc and,Nuclease
R5345 T6436 T6439 nummod 1,cells
R5346 T6437 T6439 nummod ×,cells
R5347 T6438 T6439 nummod 107,cells
R5348 T6439 T6427 dobj cells,using
R5349 T6440 T6439 prep per,cells
R5350 T6441 T6440 pobj tube,per
R5351 T6442 T6427 prep in,using
R5352 T6443 T6444 det a,volume
R5353 T6444 T6442 pobj volume,in
R5354 T6445 T6444 prep of,volume
R5355 T6446 T6447 nummod 1.5,ml
R5356 T6447 T6445 pobj ml,of
R5357 T6448 T6426 punct .,performed
R5358 T6449 T6455 prep After,stopped
R5359 T6450 T6451 nummod 2,min
R5360 T6451 T6449 pobj min,After
R5361 T6452 T6455 punct ",",stopped
R5362 T6453 T6455 nsubjpass reaction,stopped
R5363 T6454 T6455 auxpass was,stopped
R5364 T6455 T6455 ROOT stopped,stopped
R5365 T6456 T6455 prep by,stopped
R5366 T6457 T6456 pcomp adding,by
R5367 T6458 T6460 quantmod 30,200
R5368 T6459 T6460 quantmod µl,200
R5369 T6460 T6457 dobj 200,adding
R5370 T6461 T6462 compound mM,EGTA
R5371 T6462 T6457 dobj EGTA,adding
R5372 T6463 T6455 punct .,stopped
R5373 T6464 T6465 amod Further,steps
R5374 T6465 T6467 nsubjpass steps,performed
R5375 T6466 T6467 auxpass were,performed
R5376 T6467 T6467 ROOT performed,performed
R5377 T6468 T6467 advcl using,performed
R5378 T6469 T6473 det the,Kit
R5379 T6470 T6473 compound Chromatin,Kit
R5380 T6471 T6473 compound Immunoprecipitation,Kit
R5381 T6472 T6473 compound Assay,Kit
R5382 T6473 T6468 dobj Kit,using
R5383 T6474 T6473 punct (,Kit
R5384 T6475 T6473 appos Upstate,Kit
R5385 T6476 T6473 punct ),Kit
R5386 T6477 T6467 prep according,performed
R5387 T6478 T6477 prep to,according
R5388 T6479 T6480 det the,manufacturer
R5389 T6480 T6482 poss manufacturer,instructions
R5390 T6481 T6480 case 's,manufacturer
R5391 T6482 T6478 pobj instructions,to
R5392 T6483 T6467 punct .,performed
R5393 T6484 T6487 nummod 1,cells
R5394 T6485 T6487 nummod ×,cells
R5395 T6486 T6487 nummod 107,cells
R5396 T6487 T6516 nsubjpass cells,used
R5397 T6488 T6487 cc and,cells
R5398 T6489 T6491 nummod 2,antibody
R5399 T6490 T6491 compound µg,antibody
R5400 T6491 T6487 conj antibody,cells
R5401 T6492 T6516 punct (,used
R5402 T6493 T6516 nsubjpass Sp1,used
R5403 T6494 T6495 punct (,Pep2
R5404 T6495 T6493 appos Pep2,Sp1
R5405 T6496 T6495 punct ),Pep2
R5406 T6497 T6516 nsubjpass sc-59,used
R5407 T6498 T6497 punct ",",sc-59
R5408 T6499 T6497 conj Sp3,sc-59
R5409 T6500 T6499 punct (,Sp3
R5410 T6501 T6499 appos D-20,Sp3
R5411 T6502 T6499 punct ),Sp3
R5412 T6503 T6497 conj sc-644,sc-59
R5413 T6504 T6503 cc or,sc-644
R5414 T6505 T6503 conj actin,sc-644
R5415 T6506 T6507 punct (,H-196
R5416 T6507 T6505 appos H-196,actin
R5417 T6508 T6505 punct ),actin
R5418 T6509 T6505 conj sc-7210,actin
R5419 T6510 T6509 punct ",",sc-7210
R5420 T6511 T6513 compound Santa,Biotechnology
R5421 T6512 T6513 compound Cruz,Biotechnology
R5422 T6513 T6509 appos Biotechnology,sc-7210
R5423 T6514 T6509 punct ),sc-7210
R5424 T6515 T6516 auxpass were,used
R5425 T6516 T6516 ROOT used,used
R5426 T6517 T6516 prep for,used
R5427 T6518 T6519 det each,immunoprecipitation
R5428 T6519 T6517 pobj immunoprecipitation,for
R5429 T6520 T6516 punct .,used
R5430 T6521 T6522 det All,buffers
R5431 T6522 T6525 nsubjpass buffers,supplied
R5432 T6523 T6525 auxpass were,supplied
R5433 T6524 T6525 advmod freshly,supplied
R5434 T6525 T6525 ROOT supplied,supplied
R5435 T6526 T6525 prep with,supplied
R5436 T6527 T6528 compound protease,inhibitors
R5437 T6528 T6526 pobj inhibitors,with
R5438 T6529 T6525 punct .,supplied
R5439 T6530 T6539 prep After,resolved
R5440 T6531 T6532 compound phenol/chloroform,extraction
R5441 T6532 T6535 nmod extraction,precipitation
R5442 T6533 T6532 cc and,extraction
R5443 T6534 T6532 conj ethanol,extraction
R5444 T6535 T6530 pobj precipitation,After
R5445 T6536 T6539 punct ",",resolved
R5446 T6537 T6539 nsubjpass DNA,resolved
R5447 T6538 T6539 auxpass was,resolved
R5448 T6539 T6539 ROOT resolved,resolved
R5449 T6540 T6539 prep in,resolved
R5450 T6541 T6542 nummod 16,µl
R5451 T6542 T6540 pobj µl,in
R5452 T6543 T6542 appos H2O,µl
R5453 T6544 T6539 punct .,resolved
R5454 T6545 T6546 compound Immunoprecipitated,DNA
R5455 T6546 T6548 nsubjpass DNA,detected
R5456 T6547 T6548 auxpass was,detected
R5457 T6548 T6548 ROOT detected,detected
R5458 T6549 T6548 agent by,detected
R5459 T6550 T6551 amod nested,PCR
R5460 T6551 T6549 pobj PCR,by
R5461 T6552 T6548 advcl using,detected
R5462 T6553 T6554 nummod 4,µl
R5463 T6554 T6552 dobj µl,using
R5464 T6555 T6554 prep of,µl
R5465 T6556 T6558 det the,DNA
R5466 T6557 T6558 amod resolved,DNA
R5467 T6558 T6555 pobj DNA,of
R5468 T6559 T6558 prep in,DNA
R5469 T6560 T6562 det the,PCR
R5470 T6561 T6562 amod first,PCR
R5471 T6562 T6559 pobj PCR,in
R5472 T6563 T6548 punct ",",detected
R5473 T6564 T6548 cc and,detected
R5474 T6565 T6566 nummod 1,µl
R5475 T6566 T6548 conj µl,detected
R5476 T6567 T6566 prep for,µl
R5477 T6568 T6570 det the,PCR
R5478 T6569 T6570 amod nested,PCR
R5479 T6570 T6567 pobj PCR,for
R5480 T6571 T6548 punct .,detected
R5481 T6572 T6583 prep For,used
R5482 T6573 T6572 pobj amplification,For
R5483 T6574 T6573 prep of,amplification
R5484 T6575 T6577 det the,promoter
R5485 T6576 T6577 compound A3G,promoter
R5486 T6577 T6574 pobj promoter,of
R5487 T6578 T6583 punct ",",used
R5488 T6579 T6581 det the,primers
R5489 T6580 T6581 amod following,primers
R5490 T6581 T6583 nsubjpass primers,used
R5491 T6582 T6583 auxpass were,used
R5492 T6583 T6583 ROOT used,used
R5493 T6584 T6589 punct :,ccacggtggcctccgagggtga-3
R5494 T6585 T6589 ccomp ChIP3Gplus,ccacggtggcctccgagggtga-3
R5495 T6586 T6585 nummod 5,ChIP3Gplus
R5496 T6587 T6589 punct ′,ccacggtggcctccgagggtga-3
R5497 T6588 T6589 punct -,ccacggtggcctccgagggtga-3
R5498 T6589 T6590 compound ccacggtggcctccgagggtga-3,′
R5499 T6590 T6583 dobj ′,used
R5500 T6591 T6590 cc and,′
R5501 T6592 T6590 conj ChIP3Gminus,′
R5502 T6593 T6594 punct :,5
R5503 T6594 T6597 dep 5,ctctccaccatcaagacagac-3
R5504 T6595 T6597 punct ′,ctctccaccatcaagacagac-3
R5505 T6596 T6597 punct -,ctctccaccatcaagacagac-3
R5506 T6597 T6598 amod ctctccaccatcaagacagac-3,′
R5507 T6598 T6590 appos ′,′
R5508 T6599 T6600 punct (,1
R5509 T6600 T6598 appos 1,′
R5510 T6601 T6583 punct .,used
R5511 T6602 T6602 ROOT PCR,PCR
R5512 T6603 T6602 punct ),PCR
R5513 T6604 T6614 punct ;,nested
R5514 T6605 T6614 nsubj ChIP3G2plus,nested
R5515 T6606 T6605 punct :,ChIP3G2plus
R5516 T6607 T6610 dep 5,tactctccctccctgtcccca-3
R5517 T6608 T6610 punct ′,tactctccctccctgtcccca-3
R5518 T6609 T6610 punct -,tactctccctccctgtcccca-3
R5519 T6610 T6611 compound tactctccctccctgtcccca-3,′
R5520 T6611 T6605 appos ′,ChIP3G2plus
R5521 T6612 T6611 cc and,′
R5522 T6613 T6611 conj ChIP3G,′
R5523 T6614 T6614 ROOT nested,nested
R5524 T6615 T6614 cc minus,nested
R5525 T6616 T6614 punct :,nested
R5526 T6617 T6621 nummod 5,′
R5527 T6618 T6620 punct ′,aggctgatgcctccgcag-3
R5528 T6619 T6620 punct -,aggctgatgcctccgcag-3
R5529 T6620 T6621 compound aggctgatgcctccgcag-3,′
R5530 T6621 T6623 npadvmod ′,nested
R5531 T6622 T6623 punct (,nested
R5532 T6623 T6623 ROOT nested,nested
R5533 T6624 T6623 dobj PCR,nested
R5534 T6625 T6623 punct ),nested
R5535 T6626 T6614 punct .,nested
R5536 T6627 T6628 compound Taq,polymerase
R5537 T6628 T6633 nsubjpass polymerase,used
R5538 T6629 T6630 punct (,Qiagen
R5539 T6630 T6628 appos Qiagen,polymerase
R5540 T6631 T6628 punct ),polymerase
R5541 T6632 T6633 auxpass was,used
R5542 T6633 T6633 ROOT used,used
R5543 T6634 T6635 advmod together,with
R5544 T6635 T6633 prep with,used
R5545 T6636 T6639 det the,conditions
R5546 T6637 T6639 amod following,conditions
R5547 T6638 T6639 compound cycle,conditions
R5548 T6639 T6635 pobj conditions,with
R5549 T6640 T6639 punct :,conditions
R5550 T6641 T6642 nummod one,cycle
R5551 T6642 T6639 appos cycle,conditions
R5552 T6643 T6644 nummod 94,°
R5553 T6644 T6645 compound °,C
R5554 T6645 T6642 appos C,cycle
R5555 T6646 T6645 prep for,C
R5556 T6647 T6648 nummod 2,min
R5557 T6648 T6646 pobj min,for
R5558 T6649 T6645 punct ;,C
R5559 T6650 T6651 nummod 30,cycles
R5560 T6651 T6642 appos cycles,cycle
R5561 T6652 T6653 nummod 94,°
R5562 T6653 T6654 compound °,C
R5563 T6654 T6642 appos C,cycle
R5564 T6655 T6639 prep for,conditions
R5565 T6656 T6662 preconj 30,for
R5566 T6657 T6656 nmod s,30
R5567 T6658 T6657 punct ",",s
R5568 T6659 T6660 nummod 60,°
R5569 T6660 T6661 compound °,C
R5570 T6661 T6657 appos C,s
R5571 T6662 T6655 prep for,for
R5572 T6663 T6662 pobj 60,for
R5573 T6664 T6663 nmod s,60
R5574 T6665 T6664 punct ",",s
R5575 T6666 T6668 nummod 72,C
R5576 T6667 T6668 compound °,C
R5577 T6668 T6664 appos C,s
R5578 T6669 T6663 prep for,60
R5579 T6670 T6671 nummod 2,min
R5580 T6671 T6669 pobj min,for
R5581 T6672 T6677 punct ;,C
R5582 T6673 T6674 nummod one,cycle
R5583 T6674 T6677 compound cycle,C
R5584 T6675 T6676 nummod 72,°
R5585 T6676 T6677 compound °,C
R5586 T6677 T6663 appos C,60
R5587 T6678 T6677 prep for,C
R5588 T6679 T6680 nummod 10,min
R5589 T6680 T6678 pobj min,for
R5590 T6681 T6633 punct .,used
R5591 T6682 T6694 prep As,targeted
R5592 T6683 T6685 det a,control
R5593 T6684 T6685 amod negative,control
R5594 T6685 T6682 pobj control,As
R5595 T6686 T6694 punct ",",targeted
R5596 T6687 T6688 det a,region
R5597 T6688 T6694 nsubjpass region,targeted
R5598 T6689 T6688 prep in,region
R5599 T6690 T6692 det the,gene
R5600 T6691 T6692 compound A3G,gene
R5601 T6692 T6689 pobj gene,in
R5602 T6693 T6694 auxpass was,targeted
R5603 T6694 T6694 ROOT targeted,targeted
R5604 T6695 T6694 advcl using,targeted
R5605 T6696 T6697 det the,primers
R5606 T6697 T6698 compound primers,ChIP3Gneg_plus
R5610 T6701 T6703 punct ′,taagtaccacccagagatgag-3
R5611 T6702 T6703 punct -,taagtaccacccagagatgag-3
R5612 T6703 T6704 compound taagtaccacccagagatgag-3,′
R5613 T6704 T6698 appos ′,ChIP3Gneg_plus
R5614 T6705 T6704 cc and,′
R5615 T6706 T6704 conj ChIP3Gneg_minus,′
R5616 T6707 T6708 punct :,5
R5617 T6708 T6712 nummod 5,′
R5618 T6709 T6711 punct ′,catgatcttcatggtggcacg-3
R5619 T6710 T6711 punct -,catgatcttcatggtggcacg-3
R5620 T6711 T6712 compound catgatcttcatggtggcacg-3,′
R5621 T6712 T6704 appos ′,′
R5622 T6713 T6712 prep for,′
R5623 T6714 T6716 det both,steps
R5624 T6715 T6716 compound PCR,steps
R5625 T6716 T6713 pobj steps,for
R5626 T6717 T6694 punct .,targeted
R5627 T6718 T6719 compound PCR,conditions
R5628 T6719 T6720 nsubj conditions,were
R5629 T6720 T6720 ROOT were,were
R5630 T6721 T6722 det the,same
R5631 T6722 T6720 attr same,were
R5632 T6723 T6722 prep as,same
R5633 T6724 T6723 prep for,as
R5634 T6725 T6728 det the,sequence
R5635 T6726 T6728 compound A3G,sequence
R5636 T6727 T6728 compound promoter,sequence
R5637 T6728 T6724 pobj sequence,for
R5638 T6729 T6720 punct ",",were
R5639 T6730 T6720 prep with,were
R5640 T6731 T6732 det the,exception
R5641 T6732 T6730 pobj exception,with
R5642 T6733 T6737 mark that,decreased
R5643 T6734 T6735 amod annealing,temperature
R5644 T6735 T6737 nsubjpass temperature,decreased
R5645 T6736 T6737 auxpass was,decreased
R5646 T6737 T6720 advcl decreased,were
R5647 T6738 T6737 prep to,decreased
R5648 T6739 T6741 nummod 55,C.
R5649 T6740 T6741 nummod °,C.
R5650 T6741 T6738 pobj C.,to
R5607 T6698 T6695 dobj ChIP3Gneg_plus,using
R5608 T6699 T6698 punct :,ChIP3Gneg_plus
R5609 T6700 T6703 nummod 5,taagtaccacccagagatgag-3

sentences

Id Subject Object Predicate Lexical cue
T5951 0-42 Sentence denotes Chromatin immunoprecipitation (ChIP) assay
T5952 43-281 Sentence denotes A3.01 cells were treated with RPMI culture medium containing 1% formaldehyde for 10 min. at 37°C. Cells were washed twice with ice-cold PBS and incubated for 10 min. on ice after resuspension in SDS lysis buffer (ChIP Assay Kit, Upstate).
T5953 282-424 Sentence denotes After centrifugation, pellets were resuspended in MNase reaction buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 1 mM CaCl2, 4% NP-40).
T5954 425-546 Sentence denotes DNA digestion was performed using 50 U Micrococcal Nuclease (Fermentas) and 1 × 107 cells per tube in a volume of 1.5 ml.
T5955 547-609 Sentence denotes After 2 min, reaction was stopped by adding 30 µl 200 mM EGTA.
T5956 610-744 Sentence denotes Further steps were performed using the Chromatin Immunoprecipitation Assay Kit (Upstate) according to the manufacturer's instructions.
T5957 745-902 Sentence denotes 1 × 107 cells and 2 µg antibody (Sp1(Pep2) sc-59, Sp3(D-20) sc-644 or actin(H-196) sc-7210, Santa Cruz Biotechnology) were used for each immunoprecipitation.
T5958 903-962 Sentence denotes All buffers were freshly supplied with protease inhibitors.
T5959 963-1055 Sentence denotes After phenol/chloroform extraction and ethanol precipitation, DNA was resolved in 16 µl H2O.
T5960 1056-1183 Sentence denotes Immunoprecipitated DNA was detected by nested PCR using 4 µl of the resolved DNA in the first PCR, and 1 µl for the nested PCR.
T5961 1184-1255 Sentence denotes For amplification of the A3G promoter, the following primers were used:
T5962 1256-1312 Sentence denotes ChIP3Gplus 5′-ccacggtggcctccgagggtga-3′ and ChIP3Gminus:
T5963 1313-1344 Sentence denotes 5′-ctctccaccatcaagacagac-3′ (1.
T5964 1345-1363 Sentence denotes PCR); ChIP3G2plus:
T5965 1364-1416 Sentence denotes 5′-tactctccctccctgtcccca-3′ and ChIP3G nested minus:
T5966 1417-1455 Sentence denotes 5′-aggctgatgcctccgcag-3′ (nested PCR).
T5967 1456-1643 Sentence denotes Taq polymerase (Qiagen) was used together with the following cycle conditions: one cycle 94°C for 2 min; 30 cycles 94°C for 30 s, 60°C for 60 s, 72°C for 2 min; one cycle 72°C for 10 min.
T5968 1644-1738 Sentence denotes As a negative control, a region in the A3G gene was targeted using the primers ChIP3Gneg_plus:
T5969 1739-1787 Sentence denotes 5′-taagtaccacccagagatgag-3′ and ChIP3Gneg_minus:
T5970 1788-1835 Sentence denotes 5′-catgatcttcatggtggcacg-3′ for both PCR steps.
T5971 1836-1967 Sentence denotes PCR conditions were the same as for the A3G promoter sequence, with the exception that annealing temperature was decreased to 55°C.
T99 0-42 Sentence denotes Chromatin immunoprecipitation (ChIP) assay
T100 43-140 Sentence denotes A3.01 cells were treated with RPMI culture medium containing 1% formaldehyde for 10 min. at 37°C.
T101 141-281 Sentence denotes Cells were washed twice with ice-cold PBS and incubated for 10 min. on ice after resuspension in SDS lysis buffer (ChIP Assay Kit, Upstate).
T102 282-424 Sentence denotes After centrifugation, pellets were resuspended in MNase reaction buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 1 mM CaCl2, 4% NP-40).
T103 425-546 Sentence denotes DNA digestion was performed using 50 U Micrococcal Nuclease (Fermentas) and 1 × 107 cells per tube in a volume of 1.5 ml.
T104 547-609 Sentence denotes After 2 min, reaction was stopped by adding 30 µl 200 mM EGTA.
T105 610-744 Sentence denotes Further steps were performed using the Chromatin Immunoprecipitation Assay Kit (Upstate) according to the manufacturer's instructions.
T106 745-902 Sentence denotes 1 × 107 cells and 2 µg antibody (Sp1(Pep2) sc-59, Sp3(D-20) sc-644 or actin(H-196) sc-7210, Santa Cruz Biotechnology) were used for each immunoprecipitation.
T107 903-962 Sentence denotes All buffers were freshly supplied with protease inhibitors.
T108 963-1055 Sentence denotes After phenol/chloroform extraction and ethanol precipitation, DNA was resolved in 16 µl H2O.
T109 1056-1183 Sentence denotes Immunoprecipitated DNA was detected by nested PCR using 4 µl of the resolved DNA in the first PCR, and 1 µl for the nested PCR.
T110 1184-1255 Sentence denotes For amplification of the A3G promoter, the following primers were used:
T111 1256-1312 Sentence denotes ChIP3Gplus 5′-ccacggtggcctccgagggtga-3′ and ChIP3Gminus:
T112 1313-1344 Sentence denotes 5′-ctctccaccatcaagacagac-3′ (1.
T113 1345-1363 Sentence denotes PCR); ChIP3G2plus:
T114 1364-1416 Sentence denotes 5′-tactctccctccctgtcccca-3′ and ChIP3G nested minus:
T115 1417-1455 Sentence denotes 5′-aggctgatgcctccgcag-3′ (nested PCR).
T116 1456-1643 Sentence denotes Taq polymerase (Qiagen) was used together with the following cycle conditions: one cycle 94°C for 2 min; 30 cycles 94°C for 30 s, 60°C for 60 s, 72°C for 2 min; one cycle 72°C for 10 min.
T117 1644-1738 Sentence denotes As a negative control, a region in the A3G gene was targeted using the primers ChIP3Gneg_plus:
T118 1739-1787 Sentence denotes 5′-taagtaccacccagagatgag-3′ and ChIP3Gneg_minus:
T119 1788-1835 Sentence denotes 5′-catgatcttcatggtggcacg-3′ for both PCR steps.
T120 1836-1967 Sentence denotes PCR conditions were the same as for the A3G promoter sequence, with the exception that annealing temperature was decreased to 55°C.

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T5974 0-9 NN denotes Chromatin
T5975 10-29 NN denotes immunoprecipitation
T5976 30-31 -LRB- denotes (
T5977 31-35 NN denotes ChIP
T5978 35-36 -RRB- denotes )
T5979 37-42 NN denotes assay
T5980 43-48 NN denotes A3.01
T5981 49-54 NN denotes cells
T5982 55-59 VB denotes were
T5983 60-67 VB denotes treated
T5984 68-72 IN denotes with
T5985 73-77 NN denotes RPMI
T5986 78-85 NN denotes culture
T5987 86-92 NN denotes medium
T5988 93-103 VB denotes containing
T5989 104-105 CD denotes 1
T5990 105-106 NN denotes %
T5991 107-119 NN denotes formaldehyde
T5992 120-123 IN denotes for
T5993 124-131 CD denotes 10 min.
T5994 132-134 IN denotes at
T5995 135-140 NNP denotes 37°C.
T5996 141-146 NNP denotes Cells
T5997 147-151 VB denotes were
T5998 152-158 VB denotes washed
T5999 159-164 RB denotes twice
T6000 165-169 IN denotes with
T6001 170-178 JJ denotes ice-cold
T6002 179-182 NN denotes PBS
T6003 183-186 CC denotes and
T6004 187-196 VB denotes incubated
T6005 197-200 IN denotes for
T6006 201-208 CD denotes 10 min.
T6007 209-211 IN denotes on
T6008 212-215 NN denotes ice
T6009 216-221 IN denotes after
T6010 222-234 NN denotes resuspension
T6011 235-237 IN denotes in
T6012 238-241 NN denotes SDS
T6013 242-247 NN denotes lysis
T6014 248-254 NN denotes buffer
T6015 255-256 -LRB- denotes (
T6016 256-260 NN denotes ChIP
T6017 261-266 NNP denotes Assay
T6018 267-270 NNP denotes Kit
T6019 270-271 -COMMA- denotes ,
T6020 272-279 NNP denotes Upstate
T6021 279-280 -RRB- denotes )
T6022 282-287 IN denotes After
T6023 288-302 NN denotes centrifugation
T6024 302-303 -COMMA- denotes ,
T6025 304-311 NN denotes pellets
T6026 312-316 VB denotes were
T6027 317-328 VB denotes resuspended
T6028 329-331 IN denotes in
T6029 332-337 NN denotes MNase
T6030 338-346 NN denotes reaction
T6031 347-353 NN denotes buffer
T6032 354-355 -LRB- denotes (
T6033 355-360 NN denotes 10 mM
T6034 361-369 NN denotes Tris-HCl
T6035 370-372 NN denotes pH
T6036 373-376 CD denotes 7.5
T6037 376-377 -COMMA- denotes ,
T6038 378-383 NN denotes 10 mM
T6039 384-388 NN denotes NaCl
T6040 388-389 -COMMA- denotes ,
T6041 390-394 NN denotes 3 mM
T6042 395-400 NN denotes MgCl2
T6043 400-401 -COMMA- denotes ,
T6044 402-406 NN denotes 1 mM
T6045 407-412 NN denotes CaCl2
T6046 412-413 -COMMA- denotes ,
T6047 414-415 CD denotes 4
T6048 415-416 NN denotes %
T6049 417-422 NN denotes NP-40
T6050 422-423 -RRB- denotes )
T6051 425-428 NN denotes DNA
T6052 429-438 NN denotes digestion
T6053 439-442 VB denotes was
T6054 443-452 VB denotes performed
T6055 453-458 VB denotes using
T6056 459-461 CD denotes 50
T6057 462-463 NNP denotes U
T6058 464-475 NNP denotes Micrococcal
T6059 476-484 NNP denotes Nuclease
T6060 485-486 -LRB- denotes (
T6061 486-495 NN denotes Fermentas
T6062 495-496 -RRB- denotes )
T6063 497-500 CC denotes and
T6064 501-508 CD denotes 1 × 107
T6065 509-514 NN denotes cells
T6066 515-518 IN denotes per
T6067 519-523 NN denotes tube
T6068 524-526 IN denotes in
T6069 527-528 DT denotes a
T6070 529-535 NN denotes volume
T6071 536-538 IN denotes of
T6072 539-545 NN denotes 1.5 ml
T6073 547-552 IN denotes After
T6074 553-558 NN denotes 2 min
T6075 558-559 -COMMA- denotes ,
T6076 560-568 NN denotes reaction
T6077 569-572 VB denotes was
T6078 573-580 VB denotes stopped
T6079 581-583 IN denotes by
T6080 584-590 VB denotes adding
T6081 591-596 JJ denotes 30 µl
T6082 597-603 NN denotes 200 mM
T6083 604-608 NN denotes EGTA
T6084 610-617 JJ denotes Further
T6085 618-623 NN denotes steps
T6086 624-628 VB denotes were
T6087 629-638 VB denotes performed
T6088 639-644 VB denotes using
T6089 645-648 DT denotes the
T6090 649-658 NNP denotes Chromatin
T6091 659-678 NNP denotes Immunoprecipitation
T6092 679-684 NNP denotes Assay
T6093 685-688 NN denotes Kit
T6094 689-690 -LRB- denotes (
T6095 690-697 NN denotes Upstate
T6096 697-698 -RRB- denotes )
T6097 699-708 VB denotes according
T6098 709-711 TO denotes to
T6099 712-715 DT denotes the
T6100 716-728 NN denotes manufacturer
T6101 728-730 POS denotes 's
T6102 731-743 NN denotes instructions
T6103 745-752 CD denotes 1 × 107
T6104 753-758 NN denotes cells
T6105 759-762 CC denotes and
T6106 763-767 NN denotes 2 µg
T6107 768-776 NN denotes antibody
T6108 777-778 -LRB- denotes (
T6109 778-781 NN denotes Sp1
T6110 781-782 -LRB- denotes (
T6111 782-786 NN denotes Pep2
T6112 786-787 -RRB- denotes )
T6113 788-793 NN denotes sc-59
T6114 793-794 -COMMA- denotes ,
T6115 795-798 NN denotes Sp3
T6116 798-799 -LRB- denotes (
T6117 799-803 NN denotes D-20
T6118 803-804 -RRB- denotes )
T6119 805-811 NN denotes sc-644
T6120 812-814 CC denotes or
T6121 815-820 NN denotes actin
T6122 820-821 -LRB- denotes (
T6123 821-826 NN denotes H-196
T6124 826-827 -RRB- denotes )
T6125 828-835 NN denotes sc-7210
T6126 835-836 -COMMA- denotes ,
T6127 837-842 NNP denotes Santa
T6128 843-847 NNP denotes Cruz
T6129 848-861 NNP denotes Biotechnology
T6130 861-862 -RRB- denotes )
T6131 863-867 VB denotes were
T6132 868-872 VB denotes used
T6133 873-876 IN denotes for
T6134 877-881 DT denotes each
T6135 882-901 NN denotes immunoprecipitation
T6136 903-906 DT denotes All
T6137 907-914 NN denotes buffers
T6138 915-919 VB denotes were
T6139 920-927 RB denotes freshly
T6140 928-936 VB denotes supplied
T6141 937-941 IN denotes with
T6142 942-950 NN denotes protease
T6143 951-961 NN denotes inhibitors
T6144 963-968 IN denotes After
T6145 969-986 NN denotes phenol/chloroform
T6146 987-997 NN denotes extraction
T6147 998-1001 CC denotes and
T6148 1002-1009 NN denotes ethanol
T6149 1010-1023 NN denotes precipitation
T6150 1023-1024 -COMMA- denotes ,
T6151 1025-1028 NN denotes DNA
T6152 1029-1032 VB denotes was
T6153 1033-1041 VB denotes resolved
T6154 1042-1044 IN denotes in
T6155 1045-1050 NN denotes 16 µl
T6156 1051-1054 NN denotes H2O
T6157 1056-1074 VB denotes Immunoprecipitated
T6158 1075-1078 NN denotes DNA
T6159 1079-1082 VB denotes was
T6160 1083-1091 VB denotes detected
T6161 1092-1094 IN denotes by
T6162 1095-1101 VB denotes nested
T6163 1102-1105 NN denotes PCR
T6164 1106-1111 VB denotes using
T6165 1112-1116 NN denotes 4 µl
T6166 1117-1119 IN denotes of
T6167 1120-1123 DT denotes the
T6168 1124-1132 VB denotes resolved
T6169 1133-1136 NN denotes DNA
T6170 1137-1139 IN denotes in
T6171 1140-1143 DT denotes the
T6172 1144-1149 JJ denotes first
T6173 1150-1153 NN denotes PCR
T6174 1153-1154 -COMMA- denotes ,
T6175 1155-1158 CC denotes and
T6176 1159-1163 NN denotes 1 µl
T6177 1164-1167 IN denotes for
T6178 1168-1171 DT denotes the
T6179 1172-1178 JJ denotes nested
T6180 1179-1182 NN denotes PCR
T6181 1184-1187 IN denotes For
T6182 1188-1201 NN denotes amplification
T6183 1202-1204 IN denotes of
T6184 1205-1208 DT denotes the
T6185 1209-1212 NN denotes A3G
T6186 1213-1221 NN denotes promoter
T6187 1221-1222 -COMMA- denotes ,
T6188 1223-1226 DT denotes the
T6189 1227-1236 VB denotes following
T6190 1237-1244 NN denotes primers
T6191 1245-1249 VB denotes were
T6192 1250-1254 VB denotes used
T6193 1254-1255 -COLON- denotes :
T6194 1256-1266 NN denotes ChIP3Gplus
T6195 1267-1295 CD denotes 5′-ccacggtggcctccgagggtga-3′
T6196 1296-1299 CC denotes and
T6197 1300-1311 NN denotes ChIP3Gminus
T6198 1311-1312 -COLON- denotes :
T6199 1313-1340 CD denotes 5′-ctctccaccatcaagacagac-3′
T6200 1341-1342 -LRB- denotes (
T6201 1342-1343 CD denotes 1
T6202 1345-1348 NN denotes PCR
T6203 1348-1349 -RRB- denotes )
T6204 1349-1350 -COLON- denotes ;
T6205 1351-1362 NN denotes ChIP3G2plus
T6206 1362-1363 -COLON- denotes :
T6207 1364-1391 JJ denotes 5′-tactctccctccctgtcccca-3′
T6208 1392-1395 CC denotes and
T6209 1396-1402 NN denotes ChIP3G
T6210 1403-1409 VB denotes nested
T6211 1410-1415 NN denotes minus
T6212 1415-1416 -COLON- denotes :
T6213 1417-1441 CD denotes 5′-aggctgatgcctccgcag-3′
T6214 1442-1443 -LRB- denotes (
T6215 1443-1449 VB denotes nested
T6216 1450-1453 NN denotes PCR
T6217 1453-1454 -RRB- denotes )
T6218 1456-1459 NN denotes Taq
T6219 1460-1470 NN denotes polymerase
T6220 1471-1472 -LRB- denotes (
T6221 1472-1478 NN denotes Qiagen
T6222 1478-1479 -RRB- denotes )
T6223 1480-1483 VB denotes was
T6224 1484-1488 VB denotes used
T6225 1489-1497 RB denotes together
T6226 1498-1502 IN denotes with
T6227 1503-1506 DT denotes the
T6228 1507-1516 VB denotes following
T6229 1517-1522 NN denotes cycle
T6230 1523-1533 NN denotes conditions
T6231 1533-1534 -COLON- denotes :
T6232 1535-1538 CD denotes one
T6233 1539-1544 NN denotes cycle
T6234 1545-1549 NN denotes 94°C
T6235 1550-1553 IN denotes for
T6236 1554-1559 NN denotes 2 min
T6237 1559-1560 -COLON- denotes ;
T6238 1561-1563 CD denotes 30
T6239 1564-1570 NN denotes cycles
T6240 1571-1575 NN denotes 94°C
T6241 1576-1579 IN denotes for
T6242 1580-1584 NN denotes 30 s
T6243 1584-1585 -COMMA- denotes ,
T6244 1586-1590 NN denotes 60°C
T6245 1591-1594 IN denotes for
T6246 1595-1599 NN denotes 60 s
T6247 1599-1600 -COMMA- denotes ,
T6248 1601-1605 NN denotes 72°C
T6249 1606-1609 IN denotes for
T6250 1610-1615 NN denotes 2 min
T6251 1615-1616 -COLON- denotes ;
T6252 1617-1620 CD denotes one
T6253 1621-1626 NN denotes cycle
T6254 1627-1631 NN denotes 72°C
T6255 1632-1635 IN denotes for
T6256 1636-1642 NN denotes 10 min
T6257 1644-1646 IN denotes As
T6258 1647-1648 DT denotes a
T6259 1649-1657 JJ denotes negative
T6260 1658-1665 NN denotes control
T6261 1665-1666 -COMMA- denotes ,
T6262 1667-1668 DT denotes a
T6263 1669-1675 NN denotes region
T6264 1676-1678 IN denotes in
T6265 1679-1682 DT denotes the
T6266 1683-1686 NN denotes A3G
T6267 1687-1691 NN denotes gene
T6268 1692-1695 VB denotes was
T6269 1696-1704 VB denotes targeted
T6270 1705-1710 VB denotes using
T6271 1711-1714 DT denotes the
T6272 1715-1722 NN denotes primers
T6273 1723-1737 NN denotes ChIP3Gneg_plus
T6274 1737-1738 -COLON- denotes :
T6275 1739-1766 JJ denotes 5′-taagtaccacccagagatgag-3′
T6276 1767-1770 CC denotes and
T6277 1771-1786 NN denotes ChIP3Gneg_minus
T6278 1786-1787 -COLON- denotes :
T6279 1788-1815 NN denotes 5′-catgatcttcatggtggcacg-3′
T6280 1816-1819 IN denotes for
T6281 1820-1824 DT denotes both
T6282 1825-1828 NN denotes PCR
T6283 1829-1834 NN denotes steps
T6284 1836-1839 NN denotes PCR
T6285 1840-1850 NN denotes conditions
T6286 1851-1855 VB denotes were
T6287 1856-1859 DT denotes the
T6288 1860-1864 JJ denotes same
T6289 1865-1867 IN denotes as
T6290 1868-1871 IN denotes for
T6291 1872-1875 DT denotes the
T6292 1876-1879 NN denotes A3G
T6293 1880-1888 NN denotes promoter
T6294 1889-1897 NN denotes sequence
T6295 1897-1898 -COMMA- denotes ,
T6296 1899-1903 IN denotes with
T6297 1904-1907 DT denotes the
T6298 1908-1917 NN denotes exception
T6299 1918-1922 IN denotes that
T6300 1923-1932 VB denotes annealing
T6301 1933-1944 NN denotes temperature
T6302 1945-1948 VB denotes was
T6303 1949-1958 VB denotes decreased
T6304 1959-1961 TO denotes to
T6305 1962-1966 NN denotes 55°C
R4915 T5975 T5974 arg1Of immunoprecipitation,Chromatin
R4916 T5975 T5976 arg1Of immunoprecipitation,(
R4917 T5977 T5976 arg2Of ChIP,(
R4918 T5978 T5976 arg3Of ),(
R4919 T5979 T5975 arg1Of assay,immunoprecipitation
R4920 T5981 T5980 arg1Of cells,A3.01
R4921 T5981 T5982 arg1Of cells,were
R4922 T5981 T5983 arg2Of cells,treated
R4923 T5983 T5982 arg2Of treated,were
R4924 T5983 T5984 arg1Of treated,with
R4925 T5985 T5984 arg2Of RPMI,with
R4926 T5987 T5986 arg1Of medium,culture
R4927 T5987 T5988 arg1Of medium,containing
R4928 T5987 T5994 arg1Of medium,at
R4929 T5987 T5997 arg1Of medium,were
R4930 T5987 T5998 arg2Of medium,washed
R4931 T5987 T6004 arg2Of medium,incubated
R4932 T5989 T5990 arg1Of 1,%
R4933 T5991 T5988 arg2Of formaldehyde,containing
R4934 T5991 T5989 arg1Of formaldehyde,1
R4935 T5991 T5992 arg1Of formaldehyde,for
R4936 T5993 T5992 arg2Of 10 min.,for
R4937 T5996 T5994 arg2Of Cells,at
R4938 T5996 T5995 arg1Of Cells,37°C.
R4939 T5998 T5999 arg1Of washed,twice
R4940 T5998 T6000 arg1Of washed,with
R4941 T5998 T6003 arg1Of washed,and
R4942 T6002 T6000 arg2Of PBS,with
R4943 T6002 T6001 arg1Of PBS,ice-cold
R4944 T6003 T5982 modOf and,were
R4945 T6003 T5997 arg2Of and,were
R4946 T6004 T6003 arg2Of incubated,and
R4947 T6004 T6005 arg1Of incubated,for
R4948 T6006 T6005 arg2Of 10 min.,for
R4949 T6006 T6007 arg1Of 10 min.,on
R4950 T6008 T6007 arg2Of ice,on
R4951 T6008 T6009 arg1Of ice,after
R4952 T6010 T6009 arg2Of resuspension,after
R4953 T6010 T6011 arg1Of resuspension,in
R4954 T6014 T6011 arg2Of buffer,in
R4955 T6014 T6012 arg1Of buffer,SDS
R4956 T6014 T6013 arg1Of buffer,lysis
R4957 T6014 T6015 arg1Of buffer,(
R4958 T6018 T6015 arg2Of Kit,(
R4959 T6018 T6016 arg1Of Kit,ChIP
R4960 T6018 T6017 arg1Of Kit,Assay
R4961 T6018 T6019 arg1Of Kit,","
R4962 T6020 T6019 arg2Of Upstate,","
R4963 T6021 T6015 arg3Of ),(
R4964 T6023 T6022 arg2Of centrifugation,After
R4965 T6025 T6026 arg1Of pellets,were
R4966 T6025 T6027 arg2Of pellets,resuspended
R4967 T6027 T6022 arg1Of resuspended,After
R4968 T6027 T6024 arg1Of resuspended,","
R4969 T6027 T6026 arg2Of resuspended,were
R4970 T6027 T6028 arg1Of resuspended,in
R4971 T6031 T6028 arg2Of buffer,in
R4972 T6031 T6029 arg1Of buffer,MNase
R4973 T6031 T6030 arg1Of buffer,reaction
R4974 T6031 T6032 arg1Of buffer,(
R4975 T6035 T6033 arg1Of pH,10 mM
R4976 T6035 T6034 arg1Of pH,Tris-HCl
R4977 T6035 T6036 arg1Of pH,7.5
R4978 T6035 T6037 arg1Of pH,","
R4979 T6037 T6040 arg1Of ",",","
R4980 T6039 T6037 arg2Of NaCl,","
R4981 T6039 T6038 arg1Of NaCl,10 mM
R4982 T6040 T6043 arg1Of ",",","
R4983 T6042 T6040 arg2Of MgCl2,","
R4984 T6042 T6041 arg1Of MgCl2,3 mM
R4985 T6043 T6046 arg1Of ",",","
R4986 T6045 T6043 arg2Of CaCl2,","
R4987 T6045 T6044 arg1Of CaCl2,1 mM
R4988 T6046 T6032 arg2Of ",",(
R4989 T6047 T6048 arg1Of 4,%
R4990 T6049 T6046 arg2Of NP-40,","
R4991 T6049 T6047 arg1Of NP-40,4
R4992 T6050 T6032 arg3Of ),(
R4993 T6052 T6051 arg1Of digestion,DNA
R4994 T6052 T6053 arg1Of digestion,was
R4995 T6052 T6054 arg2Of digestion,performed
R4996 T6054 T6053 arg2Of performed,was
R4997 T6055 T6054 arg3Of using,performed
R4998 T6055 T6068 arg1Of using,in
R4999 T6059 T6056 arg1Of Nuclease,50
R5000 T6059 T6057 arg1Of Nuclease,U
R5001 T6059 T6058 arg1Of Nuclease,Micrococcal
R5002 T6059 T6060 arg1Of Nuclease,(
R5003 T6059 T6063 arg1Of Nuclease,and
R5004 T6061 T6060 arg2Of Fermentas,(
R5005 T6062 T6060 arg3Of ),(
R5006 T6063 T6055 arg2Of and,using
R5007 T6065 T6063 arg2Of cells,and
R5008 T6065 T6064 arg1Of cells,1 × 107
R5009 T6065 T6066 arg1Of cells,per
R5010 T6067 T6066 arg2Of tube,per
R5011 T6070 T6068 arg2Of volume,in
R5012 T6070 T6069 arg1Of volume,a
R5013 T6070 T6071 arg1Of volume,of
R5014 T6072 T6071 arg2Of 1.5 ml,of
R5015 T6074 T6073 arg2Of 2 min,After
R5016 T6076 T6077 arg1Of reaction,was
R5017 T6076 T6078 arg2Of reaction,stopped
R5018 T6078 T6073 arg1Of stopped,After
R5019 T6078 T6075 arg1Of stopped,","
R5020 T6078 T6077 arg2Of stopped,was
R5021 T6078 T6079 arg1Of stopped,by
R5022 T6080 T6079 arg2Of adding,by
R5023 T6083 T6080 arg2Of EGTA,adding
R5024 T6083 T6081 arg1Of EGTA,30 µl
R5025 T6083 T6082 arg1Of EGTA,200 mM
R5026 T6085 T6084 arg1Of steps,Further
R5027 T6085 T6086 arg1Of steps,were
R5028 T6085 T6087 arg2Of steps,performed
R5029 T6087 T6086 arg2Of performed,were
R5030 T6088 T6087 arg3Of using,performed
R5031 T6088 T6097 arg1Of using,according
R5032 T6092 T6090 arg1Of Assay,Chromatin
R5033 T6092 T6091 arg1Of Assay,Immunoprecipitation
R5034 T6093 T6088 arg2Of Kit,using
R5035 T6093 T6089 arg1Of Kit,the
R5036 T6093 T6092 arg1Of Kit,Assay
R5037 T6093 T6094 arg1Of Kit,(
R5038 T6095 T6094 arg2Of Upstate,(
R5039 T6096 T6094 arg3Of ),(
R5040 T6098 T6097 arg2Of to,according
R5041 T6100 T6099 arg1Of manufacturer,the
R5042 T6100 T6101 arg2Of manufacturer,'s
R5043 T6102 T6098 arg2Of instructions,to
R5044 T6102 T6101 arg1Of instructions,'s
R5045 T6104 T6103 arg1Of cells,1 × 107
R5046 T6104 T6105 arg1Of cells,and
R5047 T6105 T6131 arg1Of and,were
R5048 T6105 T6132 arg2Of and,used
R5049 T6107 T6105 arg2Of antibody,and
R5050 T6107 T6106 arg1Of antibody,2 µg
R5051 T6107 T6108 arg1Of antibody,(
R5052 T6111 T6110 arg2Of Pep2,(
R5053 T6112 T6110 arg3Of ),(
R5054 T6113 T6109 arg1Of sc-59,Sp1
R5055 T6113 T6110 arg1Of sc-59,(
R5056 T6113 T6114 arg1Of sc-59,","
R5057 T6114 T6120 arg1Of ",",or
R5058 T6117 T6116 arg2Of D-20,(
R5059 T6118 T6116 arg3Of ),(
R5060 T6119 T6114 arg2Of sc-644,","
R5061 T6119 T6115 arg1Of sc-644,Sp3
R5062 T6119 T6116 arg1Of sc-644,(
R5063 T6120 T6108 arg2Of or,(
R5064 T6123 T6122 arg2Of H-196,(
R5065 T6124 T6122 arg3Of ),(
R5066 T6125 T6120 arg2Of sc-7210,or
R5067 T6125 T6121 arg1Of sc-7210,actin
R5068 T6125 T6122 arg1Of sc-7210,(
R5069 T6125 T6126 arg1Of sc-7210,","
R5070 T6129 T6126 arg2Of Biotechnology,","
R5071 T6129 T6127 arg1Of Biotechnology,Santa
R5072 T6129 T6128 arg1Of Biotechnology,Cruz
R5073 T6130 T6108 arg3Of ),(
R5074 T6132 T6131 arg2Of used,were
R5075 T6132 T6133 arg1Of used,for
R5076 T6135 T6133 arg2Of immunoprecipitation,for
R5077 T6135 T6134 arg1Of immunoprecipitation,each
R5078 T6137 T6136 arg1Of buffers,All
R5079 T6137 T6138 arg1Of buffers,were
R5080 T6137 T6140 arg2Of buffers,supplied
R5081 T6140 T6138 arg2Of supplied,were
R5082 T6140 T6139 arg1Of supplied,freshly
R5083 T6140 T6141 arg1Of supplied,with
R5084 T6143 T6141 arg2Of inhibitors,with
R5085 T6143 T6142 arg1Of inhibitors,protease
R5086 T6146 T6145 arg1Of extraction,phenol/chloroform
R5087 T6146 T6147 arg1Of extraction,and
R5088 T6147 T6144 arg2Of and,After
R5089 T6149 T6147 arg2Of precipitation,and
R5090 T6149 T6148 arg1Of precipitation,ethanol
R5091 T6151 T6152 arg1Of DNA,was
R5092 T6151 T6153 arg2Of DNA,resolved
R5093 T6153 T6144 arg1Of resolved,After
R5094 T6153 T6150 arg1Of resolved,","
R5095 T6153 T6152 arg2Of resolved,was
R5096 T6153 T6154 arg1Of resolved,in
R5097 T6156 T6154 arg2Of H2O,in
R5098 T6156 T6155 arg1Of H2O,16 µl
R5099 T6158 T6157 arg2Of DNA,Immunoprecipitated
R5100 T6158 T6159 arg1Of DNA,was
R5101 T6158 T6160 arg2Of DNA,detected
R5102 T6158 T6164 arg1Of DNA,using
R5103 T6160 T6159 arg2Of detected,was
R5104 T6160 T6164 modOf detected,using
R5105 T6163 T6160 arg1Of PCR,detected
R5106 T6163 T6161 arg2Of PCR,by
R5107 T6163 T6162 arg2Of PCR,nested
R5108 T6165 T6164 arg2Of 4 µl,using
R5109 T6165 T6166 arg1Of 4 µl,of
R5110 T6169 T6168 arg2Of DNA,resolved
R5111 T6169 T6170 arg1Of DNA,in
R5112 T6169 T6175 arg1Of DNA,and
R5113 T6173 T6170 arg2Of PCR,in
R5114 T6173 T6171 arg1Of PCR,the
R5115 T6173 T6172 arg1Of PCR,first
R5116 T6175 T6166 arg2Of and,of
R5117 T6175 T6167 arg1Of and,the
R5118 T6175 T6174 arg1Of and,","
R5119 T6176 T6175 arg2Of 1 µl,and
R5120 T6176 T6177 arg1Of 1 µl,for
R5121 T6180 T6177 arg2Of PCR,for
R5122 T6180 T6178 arg1Of PCR,the
R5123 T6180 T6179 arg1Of PCR,nested
R5124 T6182 T6181 arg2Of amplification,For
R5125 T6182 T6183 arg1Of amplification,of
R5126 T6186 T6183 arg2Of promoter,of
R5127 T6186 T6184 arg1Of promoter,the
R5128 T6186 T6185 arg1Of promoter,A3G
R5129 T6190 T6188 arg1Of primers,the
R5130 T6190 T6189 arg1Of primers,following
R5131 T6190 T6191 arg1Of primers,were
R5132 T6190 T6192 arg2Of primers,used
R5133 T6192 T6181 arg1Of used,For
R5134 T6192 T6187 arg1Of used,","
R5135 T6192 T6191 arg2Of used,were
R5136 T6192 T6193 arg1Of used,:
R5137 T6194 T6195 arg1Of ChIP3Gplus,5′-ccacggtggcctccgagggtga-3′
R5138 T6194 T6196 arg1Of ChIP3Gplus,and
R5139 T6197 T6196 arg2Of ChIP3Gminus,and
R5140 T6197 T6198 arg1Of ChIP3Gminus,:
R5141 T6199 T6200 arg1Of 5′-ctctccaccatcaagacagac-3′,(
R5142 T6199 T6201 arg1Of 5′-ctctccaccatcaagacagac-3′,1
R5143 T6202 T6203 arg1Of PCR,)
R5144 T6202 T6204 arg1Of PCR,;
R5145 T6205 T6204 arg2Of ChIP3G2plus,;
R5146 T6205 T6206 arg1Of ChIP3G2plus,:
R5147 T6207 T6208 arg1Of 5′-tactctccctccctgtcccca-3′,and
R5148 T6208 T6212 arg1Of and,:
R5149 T6210 T6209 arg1Of nested,ChIP3G
R5150 T6211 T6208 arg2Of minus,and
R5151 T6211 T6210 arg1Of minus,nested
R5152 T6213 T6214 arg1Of 5′-aggctgatgcctccgcag-3′,(
R5153 T6215 T6214 arg2Of nested,(
R5154 T6216 T6215 arg3Of PCR,nested
R5155 T6217 T6214 arg3Of ),(
R5156 T6219 T6218 arg1Of polymerase,Taq
R5157 T6219 T6220 arg1Of polymerase,(
R5158 T6219 T6223 arg1Of polymerase,was
R5159 T6219 T6224 arg2Of polymerase,used
R5160 T6221 T6220 arg2Of Qiagen,(
R5161 T6222 T6220 arg3Of ),(
R5162 T6224 T6223 arg2Of used,was
R5163 T6224 T6225 arg1Of used,together
R5164 T6224 T6226 arg1Of used,with
R5165 T6230 T6226 arg2Of conditions,with
R5166 T6230 T6227 arg1Of conditions,the
R5167 T6230 T6228 arg1Of conditions,following
R5168 T6230 T6229 arg1Of conditions,cycle
R5169 T6230 T6231 arg1Of conditions,:
R5170 T6234 T6231 arg2Of 94°C,:
R5171 T6234 T6232 arg1Of 94°C,one
R5172 T6234 T6233 arg1Of 94°C,cycle
R5173 T6234 T6235 arg1Of 94°C,for
R5174 T6234 T6237 arg1Of 94°C,;
R5175 T6236 T6235 arg2Of 2 min,for
R5176 T6239 T6238 arg1Of cycles,30
R5177 T6240 T6241 arg1Of 94°C,for
R5178 T6240 T6249 arg1Of 94°C,for
R5179 T6240 T6251 arg1Of 94°C,;
R5180 T6242 T6241 arg2Of 30 s,for
R5181 T6242 T6243 arg1Of 30 s,","
R5182 T6242 T6247 arg1Of 30 s,","
R5183 T6244 T6243 arg2Of 60°C,","
R5184 T6244 T6245 arg1Of 60°C,for
R5185 T6246 T6245 arg2Of 60 s,for
R5186 T6248 T6247 arg2Of 72°C,","
R5187 T6250 T6249 arg2Of 2 min,for
R5188 T6251 T6237 arg2Of ;,;
R5189 T6251 T6239 arg1Of ;,cycles
R5190 T6254 T6251 arg2Of 72°C,;
R5191 T6254 T6252 arg1Of 72°C,one
R5192 T6254 T6253 arg1Of 72°C,cycle
R5193 T6254 T6255 arg1Of 72°C,for
R5194 T6256 T6255 arg2Of 10 min,for
R5195 T6260 T6257 arg2Of control,As
R5196 T6260 T6258 arg1Of control,a
R5197 T6260 T6259 arg1Of control,negative
R5198 T6263 T6262 arg1Of region,a
R5199 T6263 T6264 arg1Of region,in
R5200 T6263 T6268 arg1Of region,was
R5201 T6263 T6269 arg2Of region,targeted
R5202 T6267 T6264 arg2Of gene,in
R5203 T6267 T6265 arg1Of gene,the
R5204 T6267 T6266 arg1Of gene,A3G
R5205 T6269 T6257 arg1Of targeted,As
R5206 T6269 T6261 arg1Of targeted,","
R5207 T6269 T6268 arg2Of targeted,was
R5208 T6269 T6270 modOf targeted,using
R5209 T6269 T6274 arg1Of targeted,:
R5210 T6273 T6270 arg2Of ChIP3Gneg_plus,using
R5211 T6273 T6271 arg1Of ChIP3Gneg_plus,the
R5212 T6273 T6272 arg1Of ChIP3Gneg_plus,primers
R5213 T6275 T6276 arg1Of 5′-taagtaccacccagagatgag-3′,and
R5214 T6276 T6278 arg1Of and,:
R5215 T6277 T6276 arg2Of ChIP3Gneg_minus,and
R5216 T6279 T6280 arg1Of 5′-catgatcttcatggtggcacg-3′,for
R5217 T6283 T6280 arg2Of steps,for
R5218 T6283 T6281 arg1Of steps,both
R5219 T6283 T6282 arg1Of steps,PCR
R5220 T6285 T6284 arg1Of conditions,PCR
R5221 T6285 T6286 arg1Of conditions,were
R5222 T6286 T6295 arg1Of were,","
R5223 T6286 T6296 arg1Of were,with
R5224 T6288 T6286 arg2Of same,were
R5225 T6288 T6287 arg1Of same,the
R5226 T6288 T6289 arg1Of same,as
R5227 T6289 T6290 arg1Of as,for
R5228 T6294 T6290 arg2Of sequence,for
R5229 T6294 T6291 arg1Of sequence,the
R5230 T6294 T6292 arg1Of sequence,A3G
R5231 T6294 T6293 arg1Of sequence,promoter
R5232 T6298 T6296 arg2Of exception,with
R5233 T6298 T6297 arg1Of exception,the
R5234 T6298 T6299 arg1Of exception,that
R5235 T6301 T6300 arg1Of temperature,annealing
R5236 T6301 T6302 arg1Of temperature,was
R5237 T6301 T6303 arg2Of temperature,decreased
R5238 T6303 T6299 arg2Of decreased,that
R5239 T6303 T6302 arg2Of decreased,was
R5240 T6303 T6304 arg1Of decreased,to
R5241 T6305 T6304 arg2Of 55°C,to

events-check-again

Id Subject Object Predicate Lexical cue
T6790 464-484 Protein denotes Micrococcal Nuclease
T6791 778-781 Protein denotes Sp1
T6792 795-798 Protein denotes Sp3
T6793 815-820 Protein denotes actin
T6794 1209-1212 Protein denotes A3G
T6795 1456-1470 Protein denotes Taq polymerase
T6796 1683-1686 Protein denotes A3G
T6797 1876-1879 Protein denotes A3G

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T6798 0-9 Protein denotes Chromatin
T6799 10-29 Binding denotes immunoprecipitation
T6800 332-337 Protein denotes MNase
T6801 649-658 Protein denotes Chromatin
T6802 763-776 Protein denotes 2 µg antibody
T6803 778-781 Protein denotes Sp1
T6804 782-786 Protein denotes Pep2
T6805 795-798 Protein denotes Sp3
T6806 799-803 Protein denotes D-20
T6807 815-820 Protein denotes actin
T6808 1209-1221 Protein denotes A3G promoter
T6809 1456-1470 Protein denotes Taq polymerase
T6810 1683-1691 Protein denotes A3G gene
T6811 1876-1897 Protein denotes A3G promoter sequence
R5651 T6798 T6799 themeOf Chromatin,immunoprecipitation

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T5943 464-484 Protein denotes Micrococcal Nuclease
T5944 778-781 Protein denotes Sp1
T5945 795-798 Protein denotes Sp3
T5946 815-820 Protein denotes actin
T5947 1209-1212 Protein denotes A3G
T5948 1456-1470 Protein denotes Taq polymerase
T5949 1683-1686 Protein denotes A3G
T5950 1876-1879 Protein denotes A3G

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T6306 370-372 P0A7Z4 denotes pH
T6307 778-781 P08047 denotes Sp1
T6308 795-798 Q02447 denotes Sp3
T6309 1209-1212 Q9HC16 denotes A3G
T6310 1683-1686 Q9HC16 denotes A3G
T6311 1876-1879 Q9HC16 denotes A3G