PMC:1920263 / 13045-15338
Annnotations
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4954 | 789-792 | Protein | denotes | Sp1 |
T4955 | 880-883 | Protein | denotes | Sp1 |
T4956 | 1111-1114 | Protein | denotes | Sp1 |
T4957 | 1263-1287 | Protein | denotes | T4 polynucleotide kinase |
T4958 | 1534-1554 | Protein | denotes | bovine serum albumin |
T4959 | 1995-1998 | Protein | denotes | Sp1 |
T4960 | 2008-2011 | Protein | denotes | Sp1 |
T4961 | 2107-2110 | Protein | denotes | Sp1 |
T4962 | 2158-2161 | Protein | denotes | Sp3 |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5852 | 97-102 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T5853 | 287-292 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T5854 | 2111-2119 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
T5855 | 2162-2170 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
T5856 | 2111-2119 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
T5857 | 2162-2170 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5850 | 2111-2119 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
T5851 | 2162-2170 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
UBERON-AE
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4963 | 1541-1546 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5390 | 0-15 | JJ | denotes | Electrophoretic |
T5391 | 16-24 | NN | denotes | mobility |
T5392 | 25-30 | NN | denotes | shift |
T5393 | 31-36 | NN | denotes | assay |
T5394 | 37-38 | -LRB- | denotes | ( |
T5395 | 38-42 | NNP | denotes | EMSA |
T5396 | 42-43 | -RRB- | denotes | ) |
T5397 | 44-47 | IN | denotes | For |
T5398 | 48-59 | NN | denotes | preparation |
T5399 | 60-62 | IN | denotes | of |
T5400 | 63-70 | JJ | denotes | nuclear |
T5401 | 71-79 | NNS | denotes | extracts |
T5402 | 79-80 | , | denotes | , |
T5403 | 81-82 | CD | denotes | 5 |
T5404 | 83-84 | NN | denotes | × |
T5405 | 85-88 | CD | denotes | 106 |
T5406 | 89-91 | CD | denotes | A3 |
T5407 | 91-94 | CD | denotes | .01 |
T5408 | 95-96 | NNP | denotes | T |
T5409 | 97-102 | NNS | denotes | cells |
T5410 | 103-107 | VBD | denotes | were |
T5411 | 108-114 | VBN | denotes | washed |
T5412 | 115-117 | IN | denotes | in |
T5413 | 118-122 | JJ | denotes | cold |
T5414 | 123-126 | NNP | denotes | PBS |
T5415 | 127-130 | CC | denotes | and |
T5416 | 131-142 | VBD | denotes | resuspended |
T5417 | 143-145 | IN | denotes | in |
T5418 | 146-149 | CD | denotes | 500 |
T5419 | 150-152 | NN | denotes | µl |
T5420 | 153-159 | NN | denotes | buffer |
T5421 | 160-161 | DT | denotes | A |
T5422 | 162-163 | -LRB- | denotes | ( |
T5423 | 163-165 | CD | denotes | 10 |
T5424 | 166-168 | NNP | denotes | mM |
T5425 | 169-174 | NNP | denotes | HEPES |
T5426 | 175-178 | NNP | denotes | pH7 |
T5427 | 178-180 | CD | denotes | .9 |
T5428 | 180-181 | , | denotes | , |
T5429 | 182-184 | CD | denotes | 10 |
T5430 | 185-187 | NNP | denotes | mM |
T5431 | 188-191 | NNP | denotes | KCl |
T5432 | 191-192 | , | denotes | , |
T5433 | 193-196 | CD | denotes | 0.1 |
T5434 | 197-199 | NNP | denotes | mM |
T5435 | 200-204 | NNP | denotes | EDTA |
T5436 | 204-205 | , | denotes | , |
T5437 | 206-209 | CD | denotes | 0.1 |
T5438 | 210-212 | NNP | denotes | mM |
T5439 | 213-217 | NNP | denotes | EGTA |
T5440 | 217-218 | , | denotes | , |
T5441 | 219-220 | CD | denotes | 1 |
T5442 | 221-223 | NNP | denotes | mM |
T5443 | 224-227 | NNP | denotes | DTT |
T5444 | 227-228 | , | denotes | , |
T5445 | 229-232 | CD | denotes | 0.5 |
T5446 | 233-235 | NNP | denotes | mM |
T5447 | 236-240 | NNP | denotes | PMSF |
T5448 | 240-241 | -RRB- | denotes | ) |
T5449 | 241-242 | . | denotes | . |
T5450 | 243-248 | IN | denotes | After |
T5451 | 249-259 | NN | denotes | incubation |
T5452 | 260-263 | IN | denotes | for |
T5453 | 264-266 | CD | denotes | 15 |
T5454 | 267-270 | NN | denotes | min |
T5455 | 271-273 | IN | denotes | on |
T5456 | 274-277 | NN | denotes | ice |
T5457 | 277-278 | , | denotes | , |
T5458 | 279-286 | JJ | denotes | swollen |
T5459 | 287-292 | NNS | denotes | cells |
T5460 | 293-297 | VBD | denotes | were |
T5461 | 298-305 | VBN | denotes | pressed |
T5462 | 306-308 | CD | denotes | 10 |
T5463 | 309-314 | NNS | denotes | times |
T5464 | 315-322 | IN | denotes | through |
T5465 | 323-324 | DT | denotes | a |
T5466 | 325-332 | NN | denotes | syringe |
T5467 | 333-337 | IN | denotes | with |
T5468 | 338-339 | DT | denotes | a |
T5469 | 340-343 | JJ | denotes | 26G |
T5470 | 344-350 | NN | denotes | needle |
T5471 | 351-354 | CC | denotes | and |
T5472 | 355-366 | VBN | denotes | centrifuged |
T5473 | 367-369 | IN | denotes | at |
T5474 | 370-374 | CD | denotes | 5000 |
T5475 | 375-381 | NN | denotes | r.p.m. |
T5476 | 382-385 | IN | denotes | for |
T5477 | 386-387 | CD | denotes | 5 |
T5478 | 388-391 | NN | denotes | min |
T5479 | 391-392 | . | denotes | . |
T5480 | 393-400 | NNS | denotes | Pellets |
T5481 | 401-410 | VBD | denotes | contained |
T5482 | 411-414 | DT | denotes | the |
T5483 | 415-421 | NNS | denotes | nuclei |
T5484 | 422-425 | CC | denotes | and |
T5485 | 426-430 | VBD | denotes | were |
T5486 | 431-437 | VBN | denotes | washed |
T5487 | 438-440 | IN | denotes | in |
T5488 | 441-447 | NN | denotes | buffer |
T5489 | 448-449 | DT | denotes | A |
T5490 | 450-453 | IN | denotes | for |
T5491 | 454-457 | CD | denotes | two |
T5492 | 458-463 | NNS | denotes | times |
T5493 | 464-467 | CC | denotes | and |
T5494 | 468-479 | VBN | denotes | resuspended |
T5495 | 480-482 | IN | denotes | in |
T5496 | 483-485 | CD | denotes | 50 |
T5497 | 486-488 | NN | denotes | µl |
T5498 | 489-495 | NN | denotes | buffer |
T5499 | 496-497 | NNP | denotes | C |
T5500 | 498-499 | -LRB- | denotes | ( |
T5501 | 499-501 | CD | denotes | 20 |
T5502 | 502-504 | NNP | denotes | mM |
T5503 | 505-510 | NNP | denotes | HEPES |
T5504 | 511-513 | NNP | denotes | pH |
T5505 | 514-517 | CD | denotes | 7.9 |
T5506 | 517-518 | , | denotes | , |
T5507 | 519-522 | CD | denotes | 400 |
T5508 | 523-525 | NNP | denotes | mM |
T5509 | 526-530 | NNP | denotes | NaCl |
T5510 | 530-531 | , | denotes | , |
T5511 | 532-533 | CD | denotes | 1 |
T5512 | 534-536 | NNP | denotes | mM |
T5513 | 537-541 | NNP | denotes | EDTA |
T5514 | 541-542 | , | denotes | , |
T5515 | 543-544 | CD | denotes | 1 |
T5516 | 545-547 | NNP | denotes | mM |
T5517 | 548-552 | NNP | denotes | EGTA |
T5518 | 553-554 | CD | denotes | 1 |
T5519 | 555-557 | NNP | denotes | mM |
T5520 | 558-561 | NNP | denotes | DTT |
T5521 | 561-562 | , | denotes | , |
T5522 | 563-564 | CD | denotes | 1 |
T5523 | 565-567 | NNP | denotes | mM |
T5524 | 568-572 | NNP | denotes | PMSF |
T5525 | 572-573 | -RRB- | denotes | ) |
T5526 | 573-574 | . | denotes | . |
T5527 | 575-580 | IN | denotes | After |
T5528 | 581-588 | VBG | denotes | shaking |
T5529 | 589-592 | IN | denotes | for |
T5530 | 593-595 | CD | denotes | 30 |
T5531 | 596-599 | NN | denotes | min |
T5532 | 600-602 | IN | denotes | at |
T5533 | 603-604 | CD | denotes | 4 |
T5534 | 604-605 | CD | denotes | ° |
T5535 | 605-606 | NNP | denotes | C |
T5536 | 607-610 | CC | denotes | and |
T5537 | 611-625 | NN | denotes | centrifugation |
T5538 | 626-629 | IN | denotes | for |
T5539 | 630-632 | CD | denotes | 10 |
T5540 | 633-636 | NN | denotes | min |
T5541 | 637-639 | IN | denotes | at |
T5542 | 640-642 | CD | denotes | 13 |
T5543 | 643-646 | CD | denotes | 000 |
T5544 | 647-653 | NN | denotes | r.p.m. |
T5545 | 653-654 | , | denotes | , |
T5546 | 655-667 | NNS | denotes | supernatants |
T5547 | 668-672 | VBD | denotes | were |
T5548 | 673-677 | VBN | denotes | used |
T5549 | 678-680 | IN | denotes | as |
T5550 | 681-688 | JJ | denotes | nuclear |
T5551 | 689-697 | NNS | denotes | extracts |
T5552 | 697-698 | . | denotes | . |
T5553 | 699-703 | NNP | denotes | EMSA |
T5554 | 704-710 | NNS | denotes | probes |
T5555 | 711-715 | VBD | denotes | were |
T5556 | 716-725 | VBN | denotes | generated |
T5557 | 726-728 | IN | denotes | by |
T5558 | 729-738 | VBG | denotes | annealing |
T5559 | 739-742 | DT | denotes | the |
T5560 | 743-752 | VBG | denotes | following |
T5561 | 753-766 | JJ | denotes | complementary |
T5562 | 767-783 | NNS | denotes | oligonucleotides |
T5563 | 783-784 | : | denotes | : |
T5564 | 785-792 | JJ | denotes | APO-Sp1 |
T5565 | 792-793 | NN | denotes | / |
T5566 | 793-794 | CD | denotes | 3 |
T5567 | 794-795 | , | denotes | , |
T5568 | 796-797 | CD | denotes | 5 |
T5569 | 797-798 | SYM | denotes | ′ |
T5570 | 798-799 | : | denotes | - |
T5571 | 800-832 | NN | denotes | CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3 |
T5572 | 832-833 | NN | denotes | ′ |
T5573 | 834-837 | CC | denotes | and |
T5574 | 838-839 | CD | denotes | 5 |
T5575 | 839-840 | SYM | denotes | ′ |
T5576 | 840-841 | : | denotes | - |
T5577 | 841-873 | NN | denotes | GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3 |
T5578 | 873-874 | NN | denotes | ′ |
T5579 | 874-875 | : | denotes | ; |
T5580 | 876-883 | NNP | denotes | APO-Sp1 |
T5581 | 883-884 | NNP | denotes | / |
T5582 | 884-888 | CD | denotes | 3mut |
T5583 | 888-889 | , | denotes | , |
T5584 | 890-891 | CD | denotes | 5 |
T5585 | 891-892 | SYM | denotes | ′ |
T5586 | 892-893 | : | denotes | - |
T5587 | 894-926 | NN | denotes | CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3 |
T5588 | 926-927 | NN | denotes | ′ |
T5589 | 928-931 | CC | denotes | and |
T5590 | 932-933 | CD | denotes | 5 |
T5591 | 933-934 | SYM | denotes | ′ |
T5592 | 934-935 | : | denotes | - |
T5593 | 936-968 | NN | denotes | GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3 |
T5594 | 968-969 | NN | denotes | ′ |
T5595 | 970-979 | VBG | denotes | according |
T5596 | 980-982 | TO | denotes | to |
T5597 | 983-991 | JJ | denotes | standard |
T5598 | 992-1002 | NNS | denotes | procedures |
T5599 | 1002-1003 | . | denotes | . |
T5600 | 1004-1015 | NNS | denotes | Nucleotides |
T5601 | 1016-1025 | VBG | denotes | differing |
T5602 | 1026-1030 | IN | denotes | from |
T5603 | 1031-1034 | DT | denotes | the |
T5604 | 1035-1043 | JJ | denotes | original |
T5605 | 1044-1052 | NN | denotes | promoter |
T5606 | 1053-1061 | NN | denotes | sequence |
T5607 | 1062-1065 | VBP | denotes | are |
T5608 | 1066-1071 | VBN | denotes | shown |
T5609 | 1072-1074 | IN | denotes | in |
T5610 | 1075-1079 | JJ | denotes | bold |
T5611 | 1080-1084 | NN | denotes | type |
T5612 | 1084-1085 | . | denotes | . |
T5613 | 1086-1087 | DT | denotes | A |
T5614 | 1088-1100 | RB | denotes | commercially |
T5615 | 1101-1110 | JJ | denotes | available |
T5616 | 1111-1114 | JJ | denotes | Sp1 |
T5617 | 1115-1120 | NN | denotes | probe |
T5618 | 1121-1122 | -LRB- | denotes | ( |
T5619 | 1122-1129 | JJ | denotes | sc-2502 |
T5620 | 1129-1130 | , | denotes | , |
T5621 | 1131-1139 | VBD | denotes | referred |
T5622 | 1140-1142 | TO | denotes | to |
T5623 | 1143-1145 | IN | denotes | as |
T5624 | 1146-1153 | NNS | denotes | Sp1cons |
T5625 | 1153-1154 | -RRB- | denotes | ) |
T5626 | 1155-1158 | VBD | denotes | was |
T5627 | 1159-1168 | VBN | denotes | purchased |
T5628 | 1169-1173 | IN | denotes | from |
T5629 | 1174-1179 | NNP | denotes | Santa |
T5630 | 1180-1184 | NNP | denotes | Cruz |
T5631 | 1185-1198 | NNP | denotes | Biotechnology |
T5632 | 1198-1199 | . | denotes | . |
T5633 | 1200-1203 | DT | denotes | The |
T5634 | 1204-1219 | JJ | denotes | double-stranded |
T5635 | 1220-1236 | NNS | denotes | oligonucleotides |
T5636 | 1237-1241 | VBD | denotes | were |
T5637 | 1242-1243 | CD | denotes | 5 |
T5638 | 1243-1244 | CD | denotes | ′ |
T5639 | 1245-1256 | JJ | denotes | end-labeled |
T5640 | 1257-1262 | VBG | denotes | using |
T5641 | 1263-1265 | CD | denotes | T4 |
T5642 | 1266-1280 | NN | denotes | polynucleotide |
T5643 | 1281-1287 | NN | denotes | kinase |
T5644 | 1288-1289 | -LRB- | denotes | ( |
T5645 | 1289-1292 | NNP | denotes | New |
T5646 | 1293-1300 | NNP | denotes | England |
T5647 | 1301-1308 | NNPS | denotes | Biolabs |
T5648 | 1308-1309 | -RRB- | denotes | ) |
T5649 | 1310-1313 | CC | denotes | and |
T5650 | 1314-1315 | NNP | denotes | [ |
T5651 | 1315-1320 | NNP | denotes | γ-32P |
T5652 | 1320-1321 | NNP | denotes | ] |
T5653 | 1321-1324 | NNP | denotes | ATP |
T5654 | 1325-1326 | -LRB- | denotes | ( |
T5655 | 1326-1330 | CD | denotes | 3000 |
T5656 | 1331-1338 | NNP | denotes | Ci/mmol |
T5657 | 1338-1339 | , | denotes | , |
T5658 | 1340-1348 | NNP | denotes | Amersham |
T5659 | 1348-1349 | -RRB- | denotes | ) |
T5660 | 1350-1353 | CC | denotes | and |
T5661 | 1354-1362 | VBN | denotes | purified |
T5662 | 1363-1365 | IN | denotes | by |
T5663 | 1366-1371 | VBG | denotes | using |
T5664 | 1372-1376 | NNP | denotes | Nick |
T5665 | 1377-1380 | NNP | denotes | G50 |
T5666 | 1381-1388 | NNS | denotes | columns |
T5667 | 1389-1390 | -LRB- | denotes | ( |
T5668 | 1390-1398 | NNP | denotes | Amersham |
T5669 | 1398-1399 | -RRB- | denotes | ) |
T5670 | 1399-1400 | . | denotes | . |
T5671 | 1401-1404 | IN | denotes | For |
T5672 | 1405-1409 | NNP | denotes | EMSA |
T5673 | 1409-1410 | , | denotes | , |
T5674 | 1411-1412 | CD | denotes | 5 |
T5675 | 1413-1415 | NN | denotes | µg |
T5676 | 1416-1418 | IN | denotes | of |
T5677 | 1419-1426 | JJ | denotes | nuclear |
T5678 | 1427-1435 | NNS | denotes | proteins |
T5679 | 1436-1440 | VBD | denotes | were |
T5680 | 1441-1453 | VBN | denotes | preincubated |
T5681 | 1454-1456 | IN | denotes | on |
T5682 | 1457-1460 | NN | denotes | ice |
T5683 | 1461-1465 | IN | denotes | with |
T5684 | 1466-1467 | CD | denotes | 2 |
T5685 | 1468-1470 | NN | denotes | µg |
T5686 | 1471-1473 | IN | denotes | of |
T5687 | 1474-1478 | NN | denotes | poly |
T5688 | 1478-1479 | -LRB- | denotes | ( |
T5689 | 1479-1484 | JJ | denotes | dI-dC |
T5690 | 1484-1485 | -RRB- | denotes | ) |
T5691 | 1486-1487 | -LRB- | denotes | ( |
T5692 | 1487-1492 | NNP | denotes | Roche |
T5693 | 1492-1493 | -RRB- | denotes | ) |
T5694 | 1494-1496 | IN | denotes | as |
T5695 | 1497-1499 | DT | denotes | an |
T5696 | 1500-1510 | JJ | denotes | unspecific |
T5697 | 1511-1521 | NN | denotes | competitor |
T5698 | 1522-1525 | CC | denotes | and |
T5699 | 1526-1527 | CD | denotes | 1 |
T5700 | 1528-1530 | NN | denotes | µg |
T5701 | 1531-1533 | IN | denotes | of |
T5702 | 1534-1540 | JJ | denotes | bovine |
T5703 | 1541-1546 | NN | denotes | serum |
T5704 | 1547-1554 | NN | denotes | albumin |
T5705 | 1555-1557 | IN | denotes | in |
T5706 | 1558-1562 | NN | denotes | band |
T5707 | 1563-1568 | NN | denotes | shift |
T5708 | 1569-1575 | NN | denotes | buffer |
T5709 | 1576-1577 | -LRB- | denotes | ( |
T5710 | 1577-1579 | CD | denotes | 50 |
T5711 | 1580-1582 | NNP | denotes | mM |
T5712 | 1583-1587 | NNP | denotes | Tris |
T5713 | 1587-1588 | , | denotes | , |
T5714 | 1589-1592 | CD | denotes | 150 |
T5715 | 1593-1595 | NNP | denotes | mM |
T5716 | 1596-1599 | NNP | denotes | KCl |
T5717 | 1599-1600 | , | denotes | , |
T5718 | 1601-1602 | CD | denotes | 5 |
T5719 | 1603-1605 | NNP | denotes | mM |
T5720 | 1606-1610 | NNP | denotes | EDTA |
T5721 | 1610-1611 | , | denotes | , |
T5722 | 1612-1615 | CD | denotes | 2.5 |
T5723 | 1616-1618 | NNP | denotes | mM |
T5724 | 1619-1633 | NN | denotes | dithiothreitol |
T5725 | 1633-1634 | , | denotes | , |
T5726 | 1635-1637 | CD | denotes | 20 |
T5727 | 1637-1638 | NN | denotes | % |
T5728 | 1639-1645 | NNP | denotes | Ficoll |
T5729 | 1645-1646 | -RRB- | denotes | ) |
T5730 | 1647-1650 | IN | denotes | for |
T5731 | 1651-1653 | CD | denotes | 15 |
T5732 | 1654-1657 | NN | denotes | min |
T5733 | 1657-1658 | . | denotes | . |
T5734 | 1659-1670 | JJ | denotes | 32P-labeled |
T5735 | 1671-1687 | NNS | denotes | oligonucleotides |
T5736 | 1688-1689 | -LRB- | denotes | ( |
T5737 | 1689-1691 | CD | denotes | 50 |
T5738 | 1692-1695 | CD | denotes | 000 |
T5739 | 1696-1702 | NN | denotes | c.p.m. |
T5740 | 1702-1703 | -RRB- | denotes | ) |
T5741 | 1704-1708 | VBD | denotes | were |
T5742 | 1709-1714 | VBN | denotes | added |
T5743 | 1715-1717 | IN | denotes | in |
T5744 | 1718-1719 | DT | denotes | a |
T5745 | 1720-1725 | JJ | denotes | total |
T5746 | 1726-1732 | NN | denotes | volume |
T5747 | 1733-1735 | IN | denotes | of |
T5748 | 1736-1738 | CD | denotes | 20 |
T5749 | 1739-1741 | NN | denotes | µl |
T5750 | 1741-1742 | , | denotes | , |
T5751 | 1743-1752 | VBN | denotes | incubated |
T5752 | 1753-1755 | IN | denotes | on |
T5753 | 1756-1759 | NN | denotes | ice |
T5754 | 1760-1763 | IN | denotes | for |
T5755 | 1764-1766 | CD | denotes | 20 |
T5756 | 1767-1770 | NN | denotes | min |
T5757 | 1771-1774 | CC | denotes | and |
T5758 | 1775-1781 | VBN | denotes | loaded |
T5759 | 1782-1786 | IN | denotes | onto |
T5760 | 1787-1788 | CD | denotes | 5 |
T5761 | 1788-1789 | NN | denotes | % |
T5762 | 1790-1796 | JJ | denotes | native |
T5763 | 1797-1811 | NN | denotes | polyacrylamide |
T5764 | 1812-1816 | NNS | denotes | gels |
T5765 | 1817-1819 | IN | denotes | in |
T5766 | 1820-1823 | CD | denotes | 0.5 |
T5767 | 1823-1824 | CD | denotes | × |
T5768 | 1824-1840 | NNP | denotes | Tris-borate-EDTA |
T5769 | 1841-1847 | NN | denotes | buffer |
T5770 | 1847-1848 | . | denotes | . |
T5771 | 1849-1853 | IN | denotes | Upon |
T5772 | 1854-1867 | NN | denotes | fractionation |
T5773 | 1867-1868 | , | denotes | , |
T5774 | 1869-1873 | NNS | denotes | gels |
T5775 | 1874-1878 | VBD | denotes | were |
T5776 | 1879-1884 | VBN | denotes | dried |
T5777 | 1885-1888 | CC | denotes | and |
T5778 | 1889-1896 | VBN | denotes | exposed |
T5779 | 1897-1900 | IN | denotes | for |
T5780 | 1901-1916 | NN | denotes | autoradiography |
T5781 | 1916-1917 | . | denotes | . |
T5782 | 1918-1921 | IN | denotes | For |
T5783 | 1922-1933 | NN | denotes | competition |
T5784 | 1934-1945 | NNS | denotes | experiments |
T5785 | 1945-1946 | , | denotes | , |
T5786 | 1947-1948 | LS | denotes | 1 |
T5787 | 1948-1949 | : | denotes | - |
T5788 | 1950-1952 | CC | denotes | or |
T5789 | 1953-1960 | JJ | denotes | 30-fold |
T5790 | 1961-1966 | NN | denotes | molar |
T5791 | 1967-1973 | NN | denotes | excess |
T5792 | 1974-1976 | IN | denotes | of |
T5793 | 1977-1980 | DT | denotes | the |
T5794 | 1981-1990 | JJ | denotes | unlabeled |
T5795 | 1991-1998 | JJ | denotes | APO-Sp1 |
T5796 | 1998-1999 | NN | denotes | / |
T5797 | 1999-2000 | CD | denotes | 3 |
T5798 | 2001-2003 | CC | denotes | or |
T5799 | 2004-2011 | JJ | denotes | APO-Sp1 |
T5800 | 2011-2012 | NN | denotes | / |
T5801 | 2012-2016 | CD | denotes | 3mut |
T5802 | 2017-2033 | NNS | denotes | oligonucleotides |
T5803 | 2034-2037 | VBD | denotes | was |
T5804 | 2038-2043 | VBN | denotes | added |
T5805 | 2044-2046 | TO | denotes | to |
T5806 | 2047-2050 | DT | denotes | the |
T5807 | 2051-2064 | NN | denotes | preincubation |
T5808 | 2065-2072 | NN | denotes | mixture |
T5809 | 2072-2073 | . | denotes | . |
T5810 | 2074-2077 | IN | denotes | For |
T5811 | 2078-2088 | JJ | denotes | supershift |
T5812 | 2089-2100 | NNS | denotes | experiments |
T5813 | 2100-2101 | , | denotes | , |
T5814 | 2102-2103 | CD | denotes | 2 |
T5815 | 2104-2106 | NN | denotes | µg |
T5816 | 2107-2110 | JJ | denotes | Sp1 |
T5817 | 2111-2119 | NN | denotes | antibody |
T5818 | 2120-2121 | -LRB- | denotes | ( |
T5819 | 2121-2127 | JJ | denotes | sc-59x |
T5820 | 2127-2128 | , | denotes | , |
T5821 | 2129-2134 | NNP | denotes | Santa |
T5822 | 2135-2139 | NNP | denotes | Cruz |
T5823 | 2140-2153 | NNP | denotes | Biotechnology |
T5824 | 2153-2154 | -RRB- | denotes | ) |
T5825 | 2155-2157 | CC | denotes | or |
T5826 | 2158-2161 | JJ | denotes | Sp3 |
T5827 | 2162-2170 | NN | denotes | antibody |
T5828 | 2171-2172 | -LRB- | denotes | ( |
T5829 | 2172-2179 | JJ | denotes | sc-644x |
T5830 | 2179-2180 | , | denotes | , |
T5831 | 2181-2186 | NNP | denotes | Santa |
T5832 | 2187-2191 | NNP | denotes | Cruz |
T5833 | 2192-2205 | NNP | denotes | Biotechnology |
T5834 | 2205-2206 | -RRB- | denotes | ) |
T5835 | 2207-2211 | VBD | denotes | were |
T5836 | 2212-2217 | VBN | denotes | added |
T5837 | 2218-2220 | TO | denotes | to |
T5838 | 2221-2224 | DT | denotes | the |
T5839 | 2225-2238 | NN | denotes | preincubation |
T5840 | 2239-2246 | NN | denotes | mixture |
T5841 | 2247-2250 | CC | denotes | and |
T5842 | 2251-2264 | NN | denotes | preincubation |
T5843 | 2265-2269 | NN | denotes | time |
T5844 | 2270-2273 | VBD | denotes | was |
T5845 | 2274-2282 | VBN | denotes | extended |
T5846 | 2283-2285 | TO | denotes | to |
T5847 | 2286-2288 | CD | denotes | 30 |
T5848 | 2289-2292 | NN | denotes | min |
T5849 | 2292-2293 | . | denotes | . |
R4455 | T5390 | T5393 | amod | Electrophoretic,assay |
R4456 | T5391 | T5392 | compound | mobility,shift |
R4457 | T5392 | T5393 | compound | shift,assay |
R4458 | T5393 | T5393 | ROOT | assay,assay |
R4459 | T5394 | T5393 | punct | (,assay |
R4460 | T5395 | T5393 | appos | EMSA,assay |
R4461 | T5396 | T5393 | punct | ),assay |
R4462 | T5397 | T5411 | prep | For,washed |
R4463 | T5398 | T5397 | pobj | preparation,For |
R4464 | T5399 | T5398 | prep | of,preparation |
R4465 | T5400 | T5401 | amod | nuclear,extracts |
R4466 | T5401 | T5399 | pobj | extracts,of |
R4467 | T5402 | T5411 | punct | ",",washed |
R4468 | T5403 | T5409 | nummod | 5,cells |
R4469 | T5404 | T5406 | punct | ×,A3 |
R4470 | T5405 | T5404 | nummod | 106,× |
R4471 | T5406 | T5409 | nummod | A3,cells |
R4472 | T5407 | T5409 | nummod | .01,cells |
R4473 | T5408 | T5409 | compound | T,cells |
R4474 | T5409 | T5411 | nsubjpass | cells,washed |
R4475 | T5410 | T5411 | auxpass | were,washed |
R4476 | T5411 | T5411 | ROOT | washed,washed |
R4477 | T5412 | T5411 | prep | in,washed |
R4478 | T5413 | T5414 | amod | cold,PBS |
R4479 | T5414 | T5412 | pobj | PBS,in |
R4480 | T5415 | T5411 | cc | and,washed |
R4481 | T5416 | T5411 | conj | resuspended,washed |
R4482 | T5417 | T5416 | prep | in,resuspended |
R4483 | T5418 | T5419 | nummod | 500,µl |
R4484 | T5419 | T5420 | compound | µl,buffer |
R4485 | T5420 | T5421 | compound | buffer,A |
R4486 | T5421 | T5417 | pobj | A,in |
R4487 | T5422 | T5421 | punct | (,A |
R4488 | T5423 | T5426 | nummod | 10,pH7 |
R4489 | T5424 | T5426 | compound | mM,pH7 |
R4490 | T5425 | T5426 | compound | HEPES,pH7 |
R4491 | T5426 | T5421 | appos | pH7,A |
R4492 | T5427 | T5426 | appos | .9,pH7 |
R4493 | T5428 | T5426 | punct | ",",pH7 |
R4494 | T5429 | T5431 | nummod | 10,KCl |
R4495 | T5430 | T5431 | compound | mM,KCl |
R4496 | T5431 | T5426 | appos | KCl,pH7 |
R4497 | T5432 | T5431 | punct | ",",KCl |
R4498 | T5433 | T5435 | nummod | 0.1,EDTA |
R4499 | T5434 | T5435 | compound | mM,EDTA |
R4500 | T5435 | T5431 | conj | EDTA,KCl |
R4501 | T5436 | T5435 | punct | ",",EDTA |
R4502 | T5437 | T5439 | compound | 0.1,EGTA |
R4503 | T5438 | T5439 | compound | mM,EGTA |
R4504 | T5439 | T5435 | conj | EGTA,EDTA |
R4505 | T5440 | T5439 | punct | ",",EGTA |
R4506 | T5441 | T5443 | nummod | 1,DTT |
R4507 | T5442 | T5443 | compound | mM,DTT |
R4508 | T5443 | T5439 | conj | DTT,EGTA |
R4509 | T5444 | T5443 | punct | ",",DTT |
R4510 | T5445 | T5447 | nummod | 0.5,PMSF |
R4511 | T5446 | T5447 | compound | mM,PMSF |
R4512 | T5447 | T5443 | appos | PMSF,DTT |
R4513 | T5448 | T5443 | punct | ),DTT |
R4514 | T5449 | T5411 | punct | .,washed |
R4515 | T5450 | T5461 | prep | After,pressed |
R4516 | T5451 | T5450 | pobj | incubation,After |
R4517 | T5452 | T5451 | prep | for,incubation |
R4518 | T5453 | T5454 | nummod | 15,min |
R4519 | T5454 | T5452 | pobj | min,for |
R4520 | T5455 | T5454 | prep | on,min |
R4521 | T5456 | T5455 | pobj | ice,on |
R4522 | T5457 | T5461 | punct | ",",pressed |
R4523 | T5458 | T5459 | amod | swollen,cells |
R4524 | T5459 | T5461 | nsubjpass | cells,pressed |
R4525 | T5460 | T5461 | auxpass | were,pressed |
R4526 | T5461 | T5461 | ROOT | pressed,pressed |
R4527 | T5462 | T5463 | nummod | 10,times |
R4528 | T5463 | T5461 | npadvmod | times,pressed |
R4529 | T5464 | T5461 | prep | through,pressed |
R4530 | T5465 | T5466 | det | a,syringe |
R4531 | T5466 | T5464 | pobj | syringe,through |
R4532 | T5467 | T5466 | prep | with,syringe |
R4533 | T5468 | T5470 | det | a,needle |
R4534 | T5469 | T5470 | compound | 26G,needle |
R4535 | T5470 | T5467 | pobj | needle,with |
R4536 | T5471 | T5461 | cc | and,pressed |
R4537 | T5472 | T5461 | conj | centrifuged,pressed |
R4538 | T5473 | T5472 | prep | at,centrifuged |
R4539 | T5474 | T5475 | nummod | 5000,r.p.m. |
R4540 | T5475 | T5473 | pobj | r.p.m.,at |
R4541 | T5476 | T5472 | prep | for,centrifuged |
R4542 | T5477 | T5478 | nummod | 5,min |
R4543 | T5478 | T5476 | pobj | min,for |
R4544 | T5479 | T5461 | punct | .,pressed |
R4545 | T5480 | T5481 | nsubj | Pellets,contained |
R4560 | T5495 | T5494 | prep | in,resuspended |
R4561 | T5496 | T5499 | nummod | 50,C |
R4562 | T5497 | T5499 | compound | µl,C |
R4563 | T5498 | T5499 | compound | buffer,C |
R4564 | T5499 | T5495 | pobj | C,in |
R4565 | T5500 | T5499 | punct | (,C |
R4566 | T5501 | T5504 | nummod | 20,pH |
R4567 | T5502 | T5504 | compound | mM,pH |
R4568 | T5503 | T5504 | compound | HEPES,pH |
R4569 | T5504 | T5499 | appos | pH,C |
R4570 | T5505 | T5509 | nummod | 7.9,NaCl |
R4571 | T5506 | T5509 | punct | ",",NaCl |
R4572 | T5507 | T5509 | nummod | 400,NaCl |
R4573 | T5508 | T5509 | compound | mM,NaCl |
R4574 | T5509 | T5499 | appos | NaCl,C |
R4575 | T5510 | T5509 | punct | ",",NaCl |
R4576 | T5511 | T5513 | nummod | 1,EDTA |
R4577 | T5512 | T5513 | compound | mM,EDTA |
R4578 | T5513 | T5509 | conj | EDTA,NaCl |
R4579 | T5514 | T5513 | punct | ",",EDTA |
R4580 | T5515 | T5520 | nummod | 1,DTT |
R4581 | T5516 | T5517 | compound | mM,EGTA |
R4582 | T5517 | T5520 | nmod | EGTA,DTT |
R4583 | T5518 | T5520 | nummod | 1,DTT |
R4584 | T5519 | T5520 | compound | mM,DTT |
R4585 | T5520 | T5513 | conj | DTT,EDTA |
R4586 | T5521 | T5520 | punct | ",",DTT |
R4587 | T5522 | T5524 | nummod | 1,PMSF |
R4588 | T5523 | T5524 | compound | mM,PMSF |
R4589 | T5524 | T5520 | appos | PMSF,DTT |
R4590 | T5525 | T5520 | punct | ),DTT |
R4591 | T5526 | T5481 | punct | .,contained |
R4592 | T5527 | T5548 | prep | After,used |
R4593 | T5528 | T5527 | pcomp | shaking,After |
R4594 | T5529 | T5528 | prep | for,shaking |
R4595 | T5530 | T5531 | nummod | 30,min |
R4596 | T5531 | T5529 | pobj | min,for |
R4597 | T5532 | T5528 | prep | at,shaking |
R4598 | T5533 | T5534 | nummod | 4,° |
R4599 | T5534 | T5532 | pobj | °,at |
R4600 | T5535 | T5528 | npadvmod | C,shaking |
R4601 | T5536 | T5535 | cc | and,C |
R4602 | T5537 | T5535 | conj | centrifugation,C |
R4603 | T5538 | T5528 | prep | for,shaking |
R4604 | T5539 | T5540 | nummod | 10,min |
R4605 | T5540 | T5538 | pobj | min,for |
R4606 | T5541 | T5528 | prep | at,shaking |
R4607 | T5542 | T5544 | nummod | 13,r.p.m. |
R4608 | T5543 | T5544 | nummod | 000,r.p.m. |
R4609 | T5544 | T5541 | pobj | r.p.m.,at |
R4610 | T5545 | T5548 | punct | ",",used |
R4611 | T5546 | T5548 | nsubjpass | supernatants,used |
R4612 | T5547 | T5548 | auxpass | were,used |
R4613 | T5548 | T5548 | ROOT | used,used |
R4614 | T5549 | T5548 | prep | as,used |
R4615 | T5550 | T5551 | amod | nuclear,extracts |
R4616 | T5551 | T5549 | pobj | extracts,as |
R4617 | T5552 | T5548 | punct | .,used |
R4618 | T5553 | T5554 | compound | EMSA,probes |
R4619 | T5554 | T5556 | nsubjpass | probes,generated |
R4620 | T5555 | T5556 | auxpass | were,generated |
R4621 | T5556 | T5556 | ROOT | generated,generated |
R4622 | T5557 | T5556 | prep | by,generated |
R4623 | T5558 | T5557 | pcomp | annealing,by |
R4624 | T5559 | T5562 | det | the,oligonucleotides |
R4625 | T5560 | T5562 | amod | following,oligonucleotides |
R4626 | T5561 | T5562 | amod | complementary,oligonucleotides |
R4627 | T5562 | T5558 | dobj | oligonucleotides,annealing |
R4628 | T5563 | T5562 | punct | :,oligonucleotides |
R4629 | T5564 | T5565 | compound | APO-Sp1,/ |
R4630 | T5565 | T5562 | appos | /,oligonucleotides |
R4631 | T5566 | T5565 | nummod | 3,/ |
R4632 | T5567 | T5565 | punct | ",",/ |
R4633 | T5568 | T5572 | nummod | 5,′ |
R4634 | T5569 | T5571 | punct | ′,CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3 |
R4635 | T5570 | T5571 | punct | -,CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3 |
R4636 | T5571 | T5572 | compound | CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3,′ |
R4637 | T5572 | T5565 | appos | ′,/ |
R4638 | T5573 | T5572 | cc | and,′ |
R4639 | T5574 | T5577 | nummod | 5,GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3 |
R4640 | T5575 | T5577 | punct | ′,GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3 |
R4641 | T5576 | T5577 | punct | -,GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3 |
R4642 | T5577 | T5578 | compound | GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3,′ |
R4643 | T5578 | T5572 | conj | ′,′ |
R4644 | T5579 | T5565 | punct | ;,/ |
R4645 | T5580 | T5581 | compound | APO-Sp1,/ |
R4646 | T5581 | T5595 | nsubj | /,according |
R4647 | T5582 | T5581 | nummod | 3mut,/ |
R4648 | T5583 | T5581 | punct | ",",/ |
R4649 | T5584 | T5588 | nummod | 5,′ |
R4650 | T5585 | T5587 | punct | ′,CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3 |
R4651 | T5586 | T5587 | punct | -,CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3 |
R4652 | T5587 | T5588 | compound | CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3,′ |
R4653 | T5588 | T5581 | appos | ′,/ |
R4654 | T5589 | T5588 | cc | and,′ |
R4655 | T5590 | T5593 | nummod | 5,GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3 |
R4656 | T5591 | T5593 | punct | ′,GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3 |
R4657 | T5592 | T5593 | punct | -,GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3 |
R4658 | T5593 | T5594 | compound | GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3,′ |
R4661 | T5596 | T5595 | prep | to,according |
R4662 | T5597 | T5598 | amod | standard,procedures |
R4663 | T5598 | T5596 | pobj | procedures,to |
R4664 | T5599 | T5556 | punct | .,generated |
R4665 | T5600 | T5608 | nsubjpass | Nucleotides,shown |
R4666 | T5601 | T5600 | acl | differing,Nucleotides |
R4667 | T5602 | T5601 | prep | from,differing |
R4668 | T5603 | T5606 | det | the,sequence |
R4669 | T5604 | T5606 | amod | original,sequence |
R4678 | T5613 | T5617 | det | A,probe |
R4679 | T5614 | T5615 | advmod | commercially,available |
R4680 | T5615 | T5617 | amod | available,probe |
R4681 | T5616 | T5617 | amod | Sp1,probe |
R4682 | T5617 | T5627 | nsubjpass | probe,purchased |
R4683 | T5618 | T5621 | punct | (,referred |
R4684 | T5619 | T5621 | nsubj | sc-2502,referred |
R4685 | T5620 | T5621 | punct | ",",referred |
R4686 | T5621 | T5617 | parataxis | referred,probe |
R4687 | T5622 | T5621 | prep | to,referred |
R4688 | T5623 | T5621 | prep | as,referred |
R4689 | T5624 | T5623 | pobj | Sp1cons,as |
R4690 | T5625 | T5621 | punct | ),referred |
R4691 | T5626 | T5627 | auxpass | was,purchased |
R4692 | T5627 | T5627 | ROOT | purchased,purchased |
R4693 | T5628 | T5627 | prep | from,purchased |
R4694 | T5629 | T5630 | compound | Santa,Cruz |
R4695 | T5630 | T5631 | compound | Cruz,Biotechnology |
R4696 | T5631 | T5628 | pobj | Biotechnology,from |
R4697 | T5632 | T5627 | punct | .,purchased |
R4698 | T5633 | T5635 | det | The,oligonucleotides |
R4699 | T5634 | T5635 | amod | double-stranded,oligonucleotides |
R4700 | T5635 | T5636 | nsubj | oligonucleotides,were |
R4701 | T5636 | T5636 | ROOT | were,were |
R4702 | T5637 | T5639 | nummod | 5,end-labeled |
R4703 | T5638 | T5639 | nummod | ′,end-labeled |
R4704 | T5639 | T5636 | acomp | end-labeled,were |
R4705 | T5640 | T5639 | advcl | using,end-labeled |
R4706 | T5641 | T5643 | nummod | T4,kinase |
R4707 | T5642 | T5643 | compound | polynucleotide,kinase |
R4708 | T5643 | T5640 | dobj | kinase,using |
R4709 | T5644 | T5647 | punct | (,Biolabs |
R4710 | T5645 | T5646 | compound | New,England |
R4711 | T5646 | T5647 | compound | England,Biolabs |
R4712 | T5647 | T5643 | appos | Biolabs,kinase |
R4713 | T5648 | T5647 | punct | ),Biolabs |
R4714 | T5649 | T5643 | cc | and,kinase |
R4715 | T5650 | T5653 | compound | [,ATP |
R4716 | T5651 | T5653 | compound | γ-32P,ATP |
R4717 | T5652 | T5653 | compound | ],ATP |
R4718 | T5653 | T5643 | conj | ATP,kinase |
R4719 | T5654 | T5656 | punct | (,Ci/mmol |
R4720 | T5655 | T5656 | nummod | 3000,Ci/mmol |
R4721 | T5656 | T5653 | appos | Ci/mmol,ATP |
R4722 | T5657 | T5656 | punct | ",",Ci/mmol |
R4723 | T5658 | T5656 | npadvmod | Amersham,Ci/mmol |
R4724 | T5659 | T5656 | punct | ),Ci/mmol |
R4725 | T5660 | T5653 | cc | and,ATP |
R4726 | T5661 | T5653 | conj | purified,ATP |
R4727 | T5662 | T5661 | prep | by,purified |
R4728 | T5663 | T5662 | pcomp | using,by |
R4729 | T5664 | T5665 | compound | Nick,G50 |
R4730 | T5665 | T5666 | compound | G50,columns |
R4731 | T5666 | T5663 | dobj | columns,using |
R4732 | T5667 | T5666 | punct | (,columns |
R4733 | T5668 | T5666 | appos | Amersham,columns |
R4734 | T5669 | T5666 | punct | ),columns |
R4735 | T5670 | T5636 | punct | .,were |
R4736 | T5671 | T5680 | prep | For,preincubated |
R4737 | T5672 | T5671 | pobj | EMSA,For |
R4738 | T5673 | T5680 | punct | ",",preincubated |
R4748 | T5683 | T5680 | prep | with,preincubated |
R4761 | T5696 | T5697 | amod | unspecific,competitor |
R4762 | T5697 | T5694 | pobj | competitor,as |
R4763 | T5698 | T5697 | cc | and,competitor |
R4764 | T5699 | T5700 | nummod | 1,µg |
R4765 | T5700 | T5697 | conj | µg,competitor |
R4766 | T5701 | T5700 | prep | of,µg |
R4767 | T5702 | T5703 | amod | bovine,serum |
R4768 | T5703 | T5701 | pobj | serum,of |
R4769 | T5704 | T5700 | conj | albumin,µg |
R4770 | T5705 | T5704 | prep | in,albumin |
R4771 | T5706 | T5707 | compound | band,shift |
R4772 | T5707 | T5708 | compound | shift,buffer |
R4773 | T5708 | T5705 | pobj | buffer,in |
R4774 | T5709 | T5712 | punct | (,Tris |
R4775 | T5710 | T5712 | nummod | 50,Tris |
R4776 | T5711 | T5712 | compound | mM,Tris |
R4777 | T5712 | T5700 | appos | Tris,µg |
R4778 | T5713 | T5712 | punct | ",",Tris |
R4779 | T5714 | T5716 | nummod | 150,KCl |
R4780 | T5715 | T5716 | compound | mM,KCl |
R4781 | T5716 | T5712 | appos | KCl,Tris |
R4782 | T5717 | T5716 | punct | ",",KCl |
R4783 | T5718 | T5720 | nummod | 5,EDTA |
R4784 | T5719 | T5720 | compound | mM,EDTA |
R4785 | T5720 | T5716 | appos | EDTA,KCl |
R4786 | T5721 | T5720 | punct | ",",EDTA |
R4787 | T5722 | T5724 | nummod | 2.5,dithiothreitol |
R4788 | T5723 | T5724 | compound | mM,dithiothreitol |
R4789 | T5724 | T5720 | appos | dithiothreitol,EDTA |
R4790 | T5725 | T5724 | punct | ",",dithiothreitol |
R4791 | T5726 | T5727 | nummod | 20,% |
R4792 | T5727 | T5728 | compound | %,Ficoll |
R4793 | T5728 | T5724 | appos | Ficoll,dithiothreitol |
R4794 | T5729 | T5724 | punct | ),dithiothreitol |
R4795 | T5730 | T5720 | prep | for,EDTA |
R4796 | T5731 | T5732 | nummod | 15,min |
R4797 | T5732 | T5730 | pobj | min,for |
R4798 | T5733 | T5680 | punct | .,preincubated |
R4799 | T5734 | T5735 | compound | 32P-labeled,oligonucleotides |
R4800 | T5735 | T5742 | nsubjpass | oligonucleotides,added |
R4801 | T5736 | T5739 | punct | (,c.p.m. |
R4802 | T5737 | T5738 | nummod | 50,000 |
R4803 | T5738 | T5739 | nummod | 000,c.p.m. |
R4804 | T5739 | T5735 | appos | c.p.m.,oligonucleotides |
R4805 | T5740 | T5735 | punct | ),oligonucleotides |
R4806 | T5741 | T5742 | auxpass | were,added |
R4807 | T5742 | T5742 | ROOT | added,added |
R4808 | T5743 | T5742 | prep | in,added |
R4809 | T5744 | T5746 | det | a,volume |
R4810 | T5745 | T5746 | amod | total,volume |
R4811 | T5746 | T5743 | pobj | volume,in |
R4812 | T5747 | T5746 | prep | of,volume |
R4813 | T5748 | T5749 | nummod | 20,µl |
R4814 | T5749 | T5747 | pobj | µl,of |
R4815 | T5750 | T5742 | punct | ",",added |
R4827 | T5762 | T5764 | amod | native,gels |
R4828 | T5763 | T5764 | compound | polyacrylamide,gels |
R4829 | T5764 | T5759 | pobj | gels,onto |
R4830 | T5765 | T5758 | prep | in,loaded |
R4831 | T5766 | T5769 | nummod | 0.5,buffer |
R4832 | T5767 | T5769 | nummod | ×,buffer |
R4833 | T5768 | T5769 | compound | Tris-borate-EDTA,buffer |
R4834 | T5769 | T5765 | pobj | buffer,in |
R4835 | T5770 | T5742 | punct | .,added |
R4836 | T5771 | T5776 | prep | Upon,dried |
R4837 | T5772 | T5771 | pobj | fractionation,Upon |
R4838 | T5773 | T5776 | punct | ",",dried |
R4839 | T5774 | T5776 | nsubjpass | gels,dried |
R4840 | T5775 | T5776 | auxpass | were,dried |
R4841 | T5776 | T5776 | ROOT | dried,dried |
R4842 | T5777 | T5776 | cc | and,dried |
R4843 | T5778 | T5776 | conj | exposed,dried |
R4844 | T5779 | T5778 | prep | for,exposed |
R4845 | T5780 | T5779 | pobj | autoradiography,for |
R4846 | T5781 | T5776 | punct | .,dried |
R4847 | T5782 | T5804 | prep | For,added |
R4848 | T5783 | T5784 | compound | competition,experiments |
R4849 | T5784 | T5782 | pobj | experiments,For |
R4850 | T5785 | T5804 | punct | ",",added |
R4851 | T5786 | T5804 | meta | 1,added |
R4852 | T5787 | T5786 | punct | -,1 |
R4853 | T5788 | T5786 | cc | or,1 |
R4854 | T5789 | T5791 | amod | 30-fold,excess |
R4855 | T5790 | T5791 | compound | molar,excess |
R4856 | T5791 | T5804 | nsubjpass | excess,added |
R4857 | T5792 | T5791 | prep | of,excess |
R4858 | T5793 | T5804 | det | the,added |
R4859 | T5794 | T5796 | amod | unlabeled,/ |
R4860 | T5795 | T5796 | compound | APO-Sp1,/ |
R4861 | T5796 | T5804 | nsubjpass | /,added |
R4862 | T5797 | T5796 | nummod | 3,/ |
R4863 | T5798 | T5796 | cc | or,/ |
R4864 | T5799 | T5796 | conj | APO-Sp1,/ |
R4865 | T5800 | T5796 | conj | /,/ |
R4866 | T5801 | T5800 | nummod | 3mut,/ |
R4867 | T5802 | T5796 | appos | oligonucleotides,/ |
R4868 | T5803 | T5804 | auxpass | was,added |
R4869 | T5804 | T5804 | ROOT | added,added |
R4870 | T5805 | T5804 | prep | to,added |
R4871 | T5806 | T5808 | det | the,mixture |
R4872 | T5807 | T5808 | compound | preincubation,mixture |
R4873 | T5808 | T5805 | pobj | mixture,to |
R4874 | T5809 | T5804 | punct | .,added |
R4875 | T5810 | T5836 | prep | For,added |
R4876 | T5811 | T5812 | amod | supershift,experiments |
R4877 | T5812 | T5810 | pobj | experiments,For |
R4878 | T5813 | T5836 | punct | ",",added |
R4879 | T5814 | T5815 | nummod | 2,µg |
R4880 | T5815 | T5817 | nmod | µg,antibody |
R4881 | T5816 | T5817 | amod | Sp1,antibody |
R4882 | T5817 | T5823 | nmod | antibody,Biotechnology |
R4883 | T5818 | T5823 | punct | (,Biotechnology |
R4884 | T5819 | T5823 | nmod | sc-59x,Biotechnology |
R4885 | T5820 | T5823 | punct | ",",Biotechnology |
R4886 | T5821 | T5822 | compound | Santa,Cruz |
R4887 | T5822 | T5823 | compound | Cruz,Biotechnology |
R4888 | T5823 | T5836 | nsubj | Biotechnology,added |
R4889 | T5824 | T5823 | punct | ),Biotechnology |
R4890 | T5825 | T5823 | cc | or,Biotechnology |
R4891 | T5826 | T5827 | amod | Sp3,antibody |
R4892 | T5827 | T5836 | nsubjpass | antibody,added |
R4893 | T5828 | T5829 | punct | (,sc-644x |
R4894 | T5829 | T5827 | appos | sc-644x,antibody |
R4895 | T5830 | T5829 | punct | ",",sc-644x |
R4896 | T5831 | T5833 | compound | Santa,Biotechnology |
R4897 | T5832 | T5833 | compound | Cruz,Biotechnology |
R4898 | T5833 | T5829 | appos | Biotechnology,sc-644x |
R4899 | T5834 | T5836 | punct | ),added |
R4900 | T5835 | T5836 | auxpass | were,added |
R4901 | T5836 | T5836 | ROOT | added,added |
R4902 | T5837 | T5836 | prep | to,added |
R4903 | T5838 | T5840 | det | the,mixture |
R4904 | T5839 | T5840 | compound | preincubation,mixture |
R4905 | T5840 | T5843 | nmod | mixture,time |
R4906 | T5841 | T5840 | cc | and,mixture |
R4907 | T5842 | T5843 | compound | preincubation,time |
R4908 | T5843 | T5837 | pobj | time,to |
R4909 | T5844 | T5845 | auxpass | was,extended |
R4910 | T5845 | T5836 | conj | extended,added |
R4911 | T5846 | T5845 | prep | to,extended |
R4912 | T5847 | T5848 | nummod | 30,min |
R4913 | T5848 | T5846 | pobj | min,to |
R4914 | T5849 | T5836 | punct | .,added |
R4546 | T5481 | T5481 | ROOT | contained,contained |
R4547 | T5482 | T5483 | det | the,nuclei |
R4548 | T5483 | T5481 | dobj | nuclei,contained |
R4549 | T5484 | T5481 | cc | and,contained |
R4550 | T5485 | T5486 | auxpass | were,washed |
R4551 | T5486 | T5481 | conj | washed,contained |
R4552 | T5487 | T5486 | prep | in,washed |
R4553 | T5488 | T5489 | compound | buffer,A |
R4554 | T5489 | T5487 | pobj | A,in |
R4555 | T5490 | T5486 | prep | for,washed |
R4556 | T5491 | T5492 | nummod | two,times |
R4557 | T5492 | T5490 | pobj | times,for |
R4558 | T5493 | T5486 | cc | and,washed |
R4559 | T5494 | T5486 | conj | resuspended,washed |
R4659 | T5594 | T5588 | conj | ′,′ |
R4660 | T5595 | T5556 | prep | according,generated |
R4670 | T5605 | T5606 | compound | promoter,sequence |
R4671 | T5606 | T5602 | pobj | sequence,from |
R4672 | T5607 | T5608 | auxpass | are,shown |
R4673 | T5608 | T5608 | ROOT | shown,shown |
R4674 | T5609 | T5608 | prep | in,shown |
R4675 | T5610 | T5611 | amod | bold,type |
R4676 | T5611 | T5609 | pobj | type,in |
R4677 | T5612 | T5608 | punct | .,shown |
R4739 | T5674 | T5675 | nummod | 5,µg |
R4740 | T5675 | T5680 | nsubjpass | µg,preincubated |
R4741 | T5676 | T5675 | prep | of,µg |
R4742 | T5677 | T5678 | amod | nuclear,proteins |
R4743 | T5678 | T5676 | pobj | proteins,of |
R4744 | T5679 | T5680 | auxpass | were,preincubated |
R4745 | T5680 | T5680 | ROOT | preincubated,preincubated |
R4746 | T5681 | T5680 | prep | on,preincubated |
R4747 | T5682 | T5681 | pobj | ice,on |
R4749 | T5684 | T5685 | nummod | 2,µg |
R4750 | T5685 | T5683 | pobj | µg,with |
R4751 | T5686 | T5685 | prep | of,µg |
R4752 | T5687 | T5686 | pobj | poly,of |
R4753 | T5688 | T5689 | punct | (,dI-dC |
R4754 | T5689 | T5687 | appos | dI-dC,poly |
R4755 | T5690 | T5680 | punct | ),preincubated |
R4756 | T5691 | T5692 | punct | (,Roche |
R4757 | T5692 | T5680 | appos | Roche,preincubated |
R4758 | T5693 | T5692 | punct | ),Roche |
R4759 | T5694 | T5680 | prep | as,preincubated |
R4760 | T5695 | T5697 | det | an,competitor |
R4816 | T5751 | T5742 | advcl | incubated,added |
R4817 | T5752 | T5751 | prep | on,incubated |
R4818 | T5753 | T5752 | pobj | ice,on |
R4819 | T5754 | T5751 | prep | for,incubated |
R4820 | T5755 | T5756 | nummod | 20,min |
R4821 | T5756 | T5754 | pobj | min,for |
R4822 | T5757 | T5756 | cc | and,min |
R4823 | T5758 | T5742 | conj | loaded,added |
R4824 | T5759 | T5758 | prep | onto,loaded |
R4825 | T5760 | T5761 | nummod | 5,% |
R4826 | T5761 | T5764 | nmod | %,gels |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4973 | 0-43 | Sentence | denotes | Electrophoretic mobility shift assay (EMSA) |
T4974 | 44-242 | Sentence | denotes | For preparation of nuclear extracts, 5 × 106 A3.01 T cells were washed in cold PBS and resuspended in 500 µl buffer A (10 mM HEPES pH7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM PMSF). |
T4975 | 243-392 | Sentence | denotes | After incubation for 15 min on ice, swollen cells were pressed 10 times through a syringe with a 26G needle and centrifuged at 5000 r.p.m. for 5 min. |
T4976 | 393-574 | Sentence | denotes | Pellets contained the nuclei and were washed in buffer A for two times and resuspended in 50 µl buffer C (20 mM HEPES pH 7.9, 400 mM NaCl, 1 mM EDTA, 1 mM EGTA 1 mM DTT, 1 mM PMSF). |
T4977 | 575-698 | Sentence | denotes | After shaking for 30 min at 4°C and centrifugation for 10 min at 13 000 r.p.m., supernatants were used as nuclear extracts. |
T4978 | 699-1003 | Sentence | denotes | EMSA probes were generated by annealing the following complementary oligonucleotides: APO-Sp1/3, 5′- CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′ and 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′; APO-Sp1/3mut, 5′- CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′ and 5′- GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′ according to standard procedures. |
T4979 | 1004-1085 | Sentence | denotes | Nucleotides differing from the original promoter sequence are shown in bold type. |
T4980 | 1086-1400 | Sentence | denotes | A commercially available Sp1 probe (sc-2502, referred to as Sp1cons) was purchased from Santa Cruz Biotechnology. The double-stranded oligonucleotides were 5′ end-labeled using T4 polynucleotide kinase (New England Biolabs) and [γ-32P]ATP (3000 Ci/mmol, Amersham) and purified by using Nick G50 columns (Amersham). |
T4981 | 1401-1658 | Sentence | denotes | For EMSA, 5 µg of nuclear proteins were preincubated on ice with 2 µg of poly(dI-dC) (Roche) as an unspecific competitor and 1 µg of bovine serum albumin in band shift buffer (50 mM Tris, 150 mM KCl, 5 mM EDTA, 2.5 mM dithiothreitol, 20% Ficoll) for 15 min. |
T4982 | 1659-1848 | Sentence | denotes | 32P-labeled oligonucleotides (50 000 c.p.m.) were added in a total volume of 20 µl, incubated on ice for 20 min and loaded onto 5% native polyacrylamide gels in 0.5×Tris-borate-EDTA buffer. |
T4983 | 1849-1917 | Sentence | denotes | Upon fractionation, gels were dried and exposed for autoradiography. |
T4984 | 1918-2073 | Sentence | denotes | For competition experiments, 1- or 30-fold molar excess of the unlabeled APO-Sp1/3 or APO-Sp1/3mut oligonucleotides was added to the preincubation mixture. |
T4985 | 2074-2293 | Sentence | denotes | For supershift experiments, 2 µg Sp1 antibody (sc-59x, Santa Cruz Biotechnology) or Sp3 antibody (sc-644x, Santa Cruz Biotechnology) were added to the preincubation mixture and preincubation time was extended to 30 min. |
T85 | 0-43 | Sentence | denotes | Electrophoretic mobility shift assay (EMSA) |
T86 | 44-242 | Sentence | denotes | For preparation of nuclear extracts, 5 × 106 A3.01 T cells were washed in cold PBS and resuspended in 500 µl buffer A (10 mM HEPES pH7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM PMSF). |
T87 | 243-392 | Sentence | denotes | After incubation for 15 min on ice, swollen cells were pressed 10 times through a syringe with a 26G needle and centrifuged at 5000 r.p.m. for 5 min. |
T88 | 393-574 | Sentence | denotes | Pellets contained the nuclei and were washed in buffer A for two times and resuspended in 50 µl buffer C (20 mM HEPES pH 7.9, 400 mM NaCl, 1 mM EDTA, 1 mM EGTA 1 mM DTT, 1 mM PMSF). |
T89 | 575-698 | Sentence | denotes | After shaking for 30 min at 4°C and centrifugation for 10 min at 13 000 r.p.m., supernatants were used as nuclear extracts. |
T90 | 699-1003 | Sentence | denotes | EMSA probes were generated by annealing the following complementary oligonucleotides: APO-Sp1/3, 5′- CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′ and 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′; APO-Sp1/3mut, 5′- CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′ and 5′- GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′ according to standard procedures. |
T91 | 1004-1085 | Sentence | denotes | Nucleotides differing from the original promoter sequence are shown in bold type. |
T92 | 1086-1199 | Sentence | denotes | A commercially available Sp1 probe (sc-2502, referred to as Sp1cons) was purchased from Santa Cruz Biotechnology. |
T93 | 1200-1400 | Sentence | denotes | The double-stranded oligonucleotides were 5′ end-labeled using T4 polynucleotide kinase (New England Biolabs) and [γ-32P]ATP (3000 Ci/mmol, Amersham) and purified by using Nick G50 columns (Amersham). |
T94 | 1401-1658 | Sentence | denotes | For EMSA, 5 µg of nuclear proteins were preincubated on ice with 2 µg of poly(dI-dC) (Roche) as an unspecific competitor and 1 µg of bovine serum albumin in band shift buffer (50 mM Tris, 150 mM KCl, 5 mM EDTA, 2.5 mM dithiothreitol, 20% Ficoll) for 15 min. |
T95 | 1659-1848 | Sentence | denotes | 32P-labeled oligonucleotides (50 000 c.p.m.) were added in a total volume of 20 µl, incubated on ice for 20 min and loaded onto 5% native polyacrylamide gels in 0.5×Tris-borate-EDTA buffer. |
T96 | 1849-1917 | Sentence | denotes | Upon fractionation, gels were dried and exposed for autoradiography. |
T97 | 1918-2073 | Sentence | denotes | For competition experiments, 1- or 30-fold molar excess of the unlabeled APO-Sp1/3 or APO-Sp1/3mut oligonucleotides was added to the preincubation mixture. |
T98 | 2074-2293 | Sentence | denotes | For supershift experiments, 2 µg Sp1 antibody (sc-59x, Santa Cruz Biotechnology) or Sp3 antibody (sc-644x, Santa Cruz Biotechnology) were added to the preincubation mixture and preincubation time was extended to 30 min. |
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4986 | 0-15 | JJ | denotes | Electrophoretic |
T4987 | 16-24 | NN | denotes | mobility |
T4988 | 25-30 | NN | denotes | shift |
T4989 | 31-36 | NN | denotes | assay |
T4990 | 37-38 | -LRB- | denotes | ( |
T4991 | 38-42 | NN | denotes | EMSA |
T4992 | 42-43 | -RRB- | denotes | ) |
T4993 | 44-47 | IN | denotes | For |
T4994 | 48-59 | NN | denotes | preparation |
T4995 | 60-62 | IN | denotes | of |
T4996 | 63-70 | JJ | denotes | nuclear |
T4997 | 71-79 | NN | denotes | extracts |
T4998 | 79-80 | -COMMA- | denotes | , |
T4999 | 81-88 | CD | denotes | 5 × 106 |
T5000 | 89-96 | NN | denotes | A3.01 T |
T5001 | 97-102 | NN | denotes | cells |
T5002 | 103-107 | VB | denotes | were |
T5003 | 108-114 | VB | denotes | washed |
T5004 | 115-117 | IN | denotes | in |
T5005 | 118-122 | JJ | denotes | cold |
T5006 | 123-126 | NN | denotes | PBS |
T5007 | 127-130 | CC | denotes | and |
T5008 | 131-142 | VB | denotes | resuspended |
T5009 | 143-145 | IN | denotes | in |
T5010 | 146-152 | CD | denotes | 500 µl |
T5011 | 153-159 | NN | denotes | buffer |
T5012 | 160-161 | NN | denotes | A |
T5013 | 162-163 | -LRB- | denotes | ( |
T5014 | 163-168 | NN | denotes | 10 mM |
T5015 | 169-174 | NN | denotes | HEPES |
T5016 | 175-180 | NN | denotes | pH7.9 |
T5017 | 180-181 | -COMMA- | denotes | , |
T5018 | 182-187 | NN | denotes | 10 mM |
T5019 | 188-191 | NN | denotes | KCl |
T5020 | 191-192 | -COMMA- | denotes | , |
T5021 | 193-199 | NNP | denotes | 0.1 mM |
T5022 | 200-204 | NNP | denotes | EDTA |
T5023 | 204-205 | -COMMA- | denotes | , |
T5024 | 206-212 | NNP | denotes | 0.1 mM |
T5025 | 213-217 | NNP | denotes | EGTA |
T5026 | 217-218 | -COMMA- | denotes | , |
T5027 | 219-223 | NN | denotes | 1 mM |
T5028 | 224-227 | NN | denotes | DTT |
T5029 | 227-228 | -COMMA- | denotes | , |
T5030 | 229-235 | NN | denotes | 0.5 mM |
T5031 | 236-240 | NN | denotes | PMSF |
T5032 | 240-241 | -RRB- | denotes | ) |
T5033 | 243-248 | IN | denotes | After |
T5034 | 249-259 | NN | denotes | incubation |
T5035 | 260-263 | IN | denotes | for |
T5036 | 264-270 | NN | denotes | 15 min |
T5037 | 271-273 | IN | denotes | on |
T5038 | 274-277 | NN | denotes | ice |
T5039 | 277-278 | -COMMA- | denotes | , |
T5040 | 279-286 | NN | denotes | swollen |
T5041 | 287-292 | NN | denotes | cells |
T5042 | 293-297 | VB | denotes | were |
T5043 | 298-305 | VB | denotes | pressed |
T5044 | 306-308 | CD | denotes | 10 |
T5045 | 309-314 | NN | denotes | times |
T5046 | 315-322 | IN | denotes | through |
T5047 | 323-324 | DT | denotes | a |
T5048 | 325-332 | NN | denotes | syringe |
T5049 | 333-337 | IN | denotes | with |
T5050 | 338-339 | DT | denotes | a |
T5051 | 340-343 | NN | denotes | 26G |
T5052 | 344-350 | NN | denotes | needle |
T5053 | 351-354 | CC | denotes | and |
T5054 | 355-366 | VB | denotes | centrifuged |
T5055 | 367-369 | IN | denotes | at |
T5056 | 370-374 | CD | denotes | 5000 |
T5057 | 375-381 | NN | denotes | r.p.m. |
T5058 | 382-385 | IN | denotes | for |
T5059 | 386-391 | NN | denotes | 5 min |
T5060 | 393-400 | NN | denotes | Pellets |
T5061 | 401-410 | VB | denotes | contained |
T5062 | 411-414 | DT | denotes | the |
T5063 | 415-421 | NN | denotes | nuclei |
T5064 | 422-425 | CC | denotes | and |
T5065 | 426-430 | VB | denotes | were |
T5066 | 431-437 | VB | denotes | washed |
T5067 | 438-440 | IN | denotes | in |
T5068 | 441-447 | NN | denotes | buffer |
T5069 | 448-449 | NN | denotes | A |
T5070 | 450-453 | IN | denotes | for |
T5071 | 454-457 | CD | denotes | two |
T5072 | 458-463 | NN | denotes | times |
T5073 | 464-467 | CC | denotes | and |
T5074 | 468-479 | VB | denotes | resuspended |
T5075 | 480-482 | IN | denotes | in |
T5076 | 483-488 | CD | denotes | 50 µl |
T5077 | 489-495 | NN | denotes | buffer |
T5078 | 496-497 | NN | denotes | C |
T5079 | 498-499 | -LRB- | denotes | ( |
T5080 | 499-504 | NN | denotes | 20 mM |
T5081 | 505-510 | NN | denotes | HEPES |
T5082 | 511-513 | NN | denotes | pH |
T5083 | 514-517 | CD | denotes | 7.9 |
T5084 | 517-518 | -COMMA- | denotes | , |
T5085 | 519-525 | NN | denotes | 400 mM |
T5086 | 526-530 | NN | denotes | NaCl |
T5087 | 530-531 | -COMMA- | denotes | , |
T5088 | 532-536 | NN | denotes | 1 mM |
T5089 | 537-541 | NN | denotes | EDTA |
T5090 | 541-542 | -COMMA- | denotes | , |
T5091 | 543-547 | NN | denotes | 1 mM |
T5092 | 548-552 | NN | denotes | EGTA |
T5093 | 553-557 | NN | denotes | 1 mM |
T5094 | 558-561 | NN | denotes | DTT |
T5095 | 561-562 | -COMMA- | denotes | , |
T5096 | 563-567 | NN | denotes | 1 mM |
T5097 | 568-572 | NN | denotes | PMSF |
T5098 | 572-573 | -RRB- | denotes | ) |
T5099 | 575-580 | IN | denotes | After |
T5100 | 581-588 | VB | denotes | shaking |
T5101 | 589-592 | IN | denotes | for |
T5102 | 593-599 | NN | denotes | 30 min |
T5103 | 600-602 | IN | denotes | at |
T5104 | 603-606 | NN | denotes | 4°C |
T5105 | 607-610 | CC | denotes | and |
T5106 | 611-625 | NN | denotes | centrifugation |
T5107 | 626-629 | IN | denotes | for |
T5108 | 630-636 | NN | denotes | 10 min |
T5109 | 637-639 | IN | denotes | at |
T5110 | 640-642 | CD | denotes | 13 |
T5111 | 643-646 | CD | denotes | 000 |
T5112 | 647-653 | NN | denotes | r.p.m. |
T5113 | 653-654 | -COMMA- | denotes | , |
T5114 | 655-667 | NN | denotes | supernatants |
T5115 | 668-672 | VB | denotes | were |
T5116 | 673-677 | VB | denotes | used |
T5117 | 678-680 | IN | denotes | as |
T5118 | 681-688 | JJ | denotes | nuclear |
T5119 | 689-697 | NN | denotes | extracts |
T5120 | 699-703 | NN | denotes | EMSA |
T5121 | 704-710 | NN | denotes | probes |
T5122 | 711-715 | VB | denotes | were |
T5123 | 716-725 | VB | denotes | generated |
T5124 | 726-728 | IN | denotes | by |
T5125 | 729-738 | VB | denotes | annealing |
T5126 | 739-742 | DT | denotes | the |
T5127 | 743-752 | VB | denotes | following |
T5128 | 753-766 | JJ | denotes | complementary |
T5129 | 767-783 | NN | denotes | oligonucleotides |
T5130 | 783-784 | -COLON- | denotes | : |
T5131 | 785-794 | NN | denotes | APO-Sp1/3 |
T5132 | 794-795 | -COMMA- | denotes | , |
T5133 | 796-799 | JJ | denotes | 5′- |
T5134 | 800-833 | NN | denotes | CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′ |
T5135 | 834-837 | CC | denotes | and |
T5136 | 838-874 | NN | denotes | 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′ |
T5137 | 874-875 | -COLON- | denotes | ; |
T5138 | 876-888 | NN | denotes | APO-Sp1/3mut |
T5139 | 888-889 | -COMMA- | denotes | , |
T5140 | 890-893 | JJ | denotes | 5′- |
T5141 | 894-927 | NN | denotes | CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′ |
T5142 | 928-931 | CC | denotes | and |
T5143 | 932-935 | CD | denotes | 5′- |
T5144 | 936-969 | NN | denotes | GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′ |
T5145 | 970-979 | VB | denotes | according |
T5146 | 980-982 | TO | denotes | to |
T5147 | 983-991 | JJ | denotes | standard |
T5148 | 992-1002 | NN | denotes | procedures |
T5149 | 1004-1015 | NN | denotes | Nucleotides |
T5150 | 1016-1025 | VB | denotes | differing |
T5151 | 1026-1030 | IN | denotes | from |
T5152 | 1031-1034 | DT | denotes | the |
T5153 | 1035-1043 | JJ | denotes | original |
T5154 | 1044-1052 | NN | denotes | promoter |
T5155 | 1053-1061 | NN | denotes | sequence |
T5156 | 1062-1065 | VB | denotes | are |
T5157 | 1066-1071 | VB | denotes | shown |
T5158 | 1072-1074 | IN | denotes | in |
T5159 | 1075-1079 | JJ | denotes | bold |
T5160 | 1080-1084 | NN | denotes | type |
T5161 | 1086-1087 | DT | denotes | A |
T5162 | 1088-1100 | RB | denotes | commercially |
T5163 | 1101-1110 | JJ | denotes | available |
T5164 | 1111-1114 | NN | denotes | Sp1 |
T5165 | 1115-1120 | NN | denotes | probe |
T5166 | 1121-1122 | -LRB- | denotes | ( |
T5167 | 1122-1129 | NN | denotes | sc-2502 |
T5168 | 1129-1130 | -COMMA- | denotes | , |
T5169 | 1131-1139 | VB | denotes | referred |
T5170 | 1140-1142 | TO | denotes | to |
T5171 | 1143-1145 | IN | denotes | as |
T5172 | 1146-1153 | NN | denotes | Sp1cons |
T5173 | 1153-1154 | -RRB- | denotes | ) |
T5174 | 1155-1158 | VB | denotes | was |
T5175 | 1159-1168 | VB | denotes | purchased |
T5176 | 1169-1173 | IN | denotes | from |
T5177 | 1174-1179 | NNP | denotes | Santa |
T5178 | 1180-1184 | NNP | denotes | Cruz |
T5179 | 1185-1199 | NNP | denotes | Biotechnology. |
T5180 | 1200-1203 | DT | denotes | The |
T5181 | 1204-1219 | JJ | denotes | double-stranded |
T5182 | 1220-1236 | NN | denotes | oligonucleotides |
T5183 | 1237-1241 | VB | denotes | were |
T5184 | 1242-1244 | CD | denotes | 5′ |
T5185 | 1245-1256 | VB | denotes | end-labeled |
T5186 | 1257-1262 | VB | denotes | using |
T5187 | 1263-1265 | NN | denotes | T4 |
T5188 | 1266-1280 | NN | denotes | polynucleotide |
T5189 | 1281-1287 | NN | denotes | kinase |
T5190 | 1288-1289 | -LRB- | denotes | ( |
T5191 | 1289-1292 | NNP | denotes | New |
T5192 | 1293-1300 | NNP | denotes | England |
T5193 | 1301-1308 | NNP | denotes | Biolabs |
T5194 | 1308-1309 | -RRB- | denotes | ) |
T5195 | 1310-1313 | CC | denotes | and |
T5196 | 1314-1315 | -LRB- | denotes | [ |
T5197 | 1315-1320 | NN | denotes | γ-32P |
T5198 | 1320-1321 | -RRB- | denotes | ] |
T5199 | 1321-1324 | NN | denotes | ATP |
T5200 | 1325-1326 | -LRB- | denotes | ( |
T5201 | 1326-1338 | NN | denotes | 3000 Ci/mmol |
T5202 | 1338-1339 | -COMMA- | denotes | , |
T5203 | 1340-1348 | NNP | denotes | Amersham |
T5204 | 1348-1349 | -RRB- | denotes | ) |
T5205 | 1350-1353 | CC | denotes | and |
T5206 | 1354-1362 | VB | denotes | purified |
T5207 | 1363-1365 | IN | denotes | by |
T5208 | 1366-1371 | VB | denotes | using |
T5209 | 1372-1376 | NNP | denotes | Nick |
T5210 | 1377-1380 | NN | denotes | G50 |
T5211 | 1381-1388 | NN | denotes | columns |
T5212 | 1389-1390 | -LRB- | denotes | ( |
T5213 | 1390-1398 | NNP | denotes | Amersham |
T5214 | 1398-1399 | -RRB- | denotes | ) |
T5215 | 1401-1404 | IN | denotes | For |
T5216 | 1405-1409 | NN | denotes | EMSA |
T5217 | 1409-1410 | -COMMA- | denotes | , |
T5218 | 1411-1415 | NN | denotes | 5 µg |
T5219 | 1416-1418 | IN | denotes | of |
T5220 | 1419-1426 | JJ | denotes | nuclear |
T5221 | 1427-1435 | NN | denotes | proteins |
T5222 | 1436-1440 | VB | denotes | were |
T5223 | 1441-1453 | VB | denotes | preincubated |
T5224 | 1454-1456 | IN | denotes | on |
T5225 | 1457-1460 | NN | denotes | ice |
T5226 | 1461-1465 | IN | denotes | with |
T5227 | 1466-1470 | NN | denotes | 2 µg |
T5228 | 1471-1473 | IN | denotes | of |
T5229 | 1474-1478 | NN | denotes | poly |
T5230 | 1478-1479 | -LRB- | denotes | ( |
T5231 | 1479-1484 | NN | denotes | dI-dC |
T5232 | 1484-1485 | -RRB- | denotes | ) |
T5233 | 1486-1487 | -LRB- | denotes | ( |
T5234 | 1487-1492 | NNP | denotes | Roche |
T5235 | 1492-1493 | -RRB- | denotes | ) |
T5236 | 1494-1496 | IN | denotes | as |
T5237 | 1497-1499 | DT | denotes | an |
T5238 | 1500-1510 | JJ | denotes | unspecific |
T5239 | 1511-1521 | NN | denotes | competitor |
T5240 | 1522-1525 | CC | denotes | and |
T5241 | 1526-1530 | NN | denotes | 1 µg |
T5242 | 1531-1533 | IN | denotes | of |
T5243 | 1534-1540 | JJ | denotes | bovine |
T5244 | 1541-1546 | NN | denotes | serum |
T5245 | 1547-1554 | NN | denotes | albumin |
T5246 | 1555-1557 | IN | denotes | in |
T5247 | 1558-1562 | NN | denotes | band |
T5248 | 1563-1568 | NN | denotes | shift |
T5249 | 1569-1575 | NN | denotes | buffer |
T5250 | 1576-1577 | -LRB- | denotes | ( |
T5251 | 1577-1582 | NNP | denotes | 50 mM |
T5252 | 1583-1587 | NNP | denotes | Tris |
T5253 | 1587-1588 | -COMMA- | denotes | , |
T5254 | 1589-1595 | NN | denotes | 150 mM |
T5255 | 1596-1599 | NNP | denotes | KCl |
T5256 | 1599-1600 | -COMMA- | denotes | , |
T5257 | 1601-1605 | NN | denotes | 5 mM |
T5258 | 1606-1610 | NN | denotes | EDTA |
T5259 | 1610-1611 | -COMMA- | denotes | , |
T5260 | 1612-1618 | NN | denotes | 2.5 mM |
T5261 | 1619-1633 | NN | denotes | dithiothreitol |
T5262 | 1633-1634 | -COMMA- | denotes | , |
T5263 | 1635-1637 | CD | denotes | 20 |
T5264 | 1637-1638 | NN | denotes | % |
T5265 | 1639-1645 | NN | denotes | Ficoll |
T5266 | 1645-1646 | -RRB- | denotes | ) |
T5267 | 1647-1650 | IN | denotes | for |
T5268 | 1651-1657 | NN | denotes | 15 min |
T5269 | 1659-1670 | JJ | denotes | 32P-labeled |
T5270 | 1671-1687 | NN | denotes | oligonucleotides |
T5271 | 1688-1689 | -LRB- | denotes | ( |
T5272 | 1689-1691 | CD | denotes | 50 |
T5273 | 1692-1702 | CD | denotes | 000 c.p.m. |
T5274 | 1702-1703 | -RRB- | denotes | ) |
T5275 | 1704-1708 | VB | denotes | were |
T5276 | 1709-1714 | VB | denotes | added |
T5277 | 1715-1717 | IN | denotes | in |
T5278 | 1718-1719 | DT | denotes | a |
T5279 | 1720-1725 | JJ | denotes | total |
T5280 | 1726-1732 | NN | denotes | volume |
T5281 | 1733-1735 | IN | denotes | of |
T5282 | 1736-1741 | NN | denotes | 20 µl |
T5283 | 1741-1742 | -COMMA- | denotes | , |
T5284 | 1743-1752 | VB | denotes | incubated |
T5285 | 1753-1755 | IN | denotes | on |
T5286 | 1756-1759 | NN | denotes | ice |
T5287 | 1760-1763 | IN | denotes | for |
T5288 | 1764-1770 | NN | denotes | 20 min |
T5289 | 1771-1774 | CC | denotes | and |
T5290 | 1775-1781 | VB | denotes | loaded |
T5291 | 1782-1786 | IN | denotes | onto |
T5292 | 1787-1788 | CD | denotes | 5 |
T5293 | 1788-1789 | NN | denotes | % |
T5294 | 1790-1796 | JJ | denotes | native |
T5295 | 1797-1811 | NN | denotes | polyacrylamide |
T5296 | 1812-1816 | NN | denotes | gels |
T5297 | 1817-1819 | IN | denotes | in |
T5298 | 1820-1840 | NN | denotes | 0.5×Tris-borate-EDTA |
T5299 | 1841-1847 | NN | denotes | buffer |
T5300 | 1849-1853 | IN | denotes | Upon |
T5301 | 1854-1867 | NN | denotes | fractionation |
T5302 | 1867-1868 | -COMMA- | denotes | , |
T5303 | 1869-1873 | NN | denotes | gels |
T5304 | 1874-1878 | VB | denotes | were |
T5305 | 1879-1884 | VB | denotes | dried |
T5306 | 1885-1888 | CC | denotes | and |
T5307 | 1889-1896 | VB | denotes | exposed |
T5308 | 1897-1900 | IN | denotes | for |
T5309 | 1901-1916 | NN | denotes | autoradiography |
T5310 | 1918-1921 | IN | denotes | For |
T5311 | 1922-1933 | NN | denotes | competition |
T5312 | 1934-1945 | NN | denotes | experiments |
T5313 | 1945-1946 | -COMMA- | denotes | , |
T5314 | 1947-1949 | CD | denotes | 1- |
T5315 | 1950-1952 | CC | denotes | or |
T5316 | 1953-1960 | RB | denotes | 30-fold |
T5317 | 1961-1966 | JJ | denotes | molar |
T5318 | 1967-1973 | NN | denotes | excess |
T5319 | 1974-1976 | IN | denotes | of |
T5320 | 1977-1980 | DT | denotes | the |
T5321 | 1981-1990 | JJ | denotes | unlabeled |
T5322 | 1991-2000 | NN | denotes | APO-Sp1/3 |
T5323 | 2001-2003 | CC | denotes | or |
T5324 | 2004-2016 | NN | denotes | APO-Sp1/3mut |
T5325 | 2017-2033 | NN | denotes | oligonucleotides |
T5326 | 2034-2037 | VB | denotes | was |
T5327 | 2038-2043 | VB | denotes | added |
T5328 | 2044-2046 | TO | denotes | to |
T5329 | 2047-2050 | DT | denotes | the |
T5330 | 2051-2064 | NN | denotes | preincubation |
T5331 | 2065-2072 | NN | denotes | mixture |
T5332 | 2074-2077 | IN | denotes | For |
T5333 | 2078-2088 | NN | denotes | supershift |
T5334 | 2089-2100 | NN | denotes | experiments |
T5335 | 2100-2101 | -COMMA- | denotes | , |
T5336 | 2102-2106 | NN | denotes | 2 µg |
T5337 | 2107-2110 | NN | denotes | Sp1 |
T5338 | 2111-2119 | NN | denotes | antibody |
T5339 | 2120-2121 | -LRB- | denotes | ( |
T5340 | 2121-2127 | NN | denotes | sc-59x |
T5341 | 2127-2128 | -COMMA- | denotes | , |
T5342 | 2129-2134 | NNP | denotes | Santa |
T5343 | 2135-2139 | NNP | denotes | Cruz |
T5344 | 2140-2153 | NNP | denotes | Biotechnology |
T5345 | 2153-2154 | -RRB- | denotes | ) |
T5346 | 2155-2157 | CC | denotes | or |
T5347 | 2158-2161 | NN | denotes | Sp3 |
T5348 | 2162-2170 | NN | denotes | antibody |
T5349 | 2171-2172 | -LRB- | denotes | ( |
T5350 | 2172-2179 | NN | denotes | sc-644x |
T5351 | 2179-2180 | -COMMA- | denotes | , |
T5352 | 2181-2186 | NNP | denotes | Santa |
T5353 | 2187-2191 | NNP | denotes | Cruz |
T5354 | 2192-2205 | NNP | denotes | Biotechnology |
T5355 | 2205-2206 | -RRB- | denotes | ) |
T5356 | 2207-2211 | VB | denotes | were |
T5357 | 2212-2217 | VB | denotes | added |
T5358 | 2218-2220 | TO | denotes | to |
T5359 | 2221-2224 | DT | denotes | the |
T5360 | 2225-2238 | NN | denotes | preincubation |
T5361 | 2239-2246 | NN | denotes | mixture |
T5362 | 2247-2250 | CC | denotes | and |
T5363 | 2251-2264 | NN | denotes | preincubation |
T5364 | 2265-2269 | NN | denotes | time |
T5365 | 2270-2273 | VB | denotes | was |
T5366 | 2274-2282 | VB | denotes | extended |
T5367 | 2283-2285 | TO | denotes | to |
T5368 | 2286-2292 | VB | denotes | 30 min |
R4059 | T4989 | T4986 | arg1Of | assay,Electrophoretic |
R4060 | T4989 | T4987 | arg1Of | assay,mobility |
R4061 | T4989 | T4988 | arg1Of | assay,shift |
R4062 | T4989 | T4990 | arg1Of | assay,( |
R4063 | T4991 | T4990 | arg2Of | EMSA,( |
R4064 | T4992 | T4990 | arg3Of | ),( |
R4065 | T4994 | T4993 | arg2Of | preparation,For |
R4066 | T4994 | T4995 | arg1Of | preparation,of |
R4067 | T4997 | T4995 | arg2Of | extracts,of |
R4068 | T4997 | T4996 | arg1Of | extracts,nuclear |
R4069 | T5001 | T4999 | arg1Of | cells,5 × 106 |
R4070 | T5001 | T5000 | arg1Of | cells,A3.01 T |
R4071 | T5001 | T5002 | arg1Of | cells,were |
R4072 | T5001 | T5003 | arg2Of | cells,washed |
R4073 | T5001 | T5008 | arg1Of | cells,resuspended |
R4074 | T5003 | T5002 | arg2Of | washed,were |
R4075 | T5003 | T5004 | arg1Of | washed,in |
R4076 | T5003 | T5007 | arg1Of | washed,and |
R4077 | T5006 | T5004 | arg2Of | PBS,in |
R4078 | T5006 | T5005 | arg1Of | PBS,cold |
R4079 | T5007 | T4993 | arg1Of | and,For |
R4080 | T5007 | T4998 | arg1Of | and,"," |
R4081 | T5008 | T5007 | arg2Of | resuspended,and |
R4082 | T5008 | T5009 | arg1Of | resuspended,in |
R4083 | T5012 | T5009 | arg2Of | A,in |
R4084 | T5012 | T5010 | arg1Of | A,500 µl |
R4085 | T5012 | T5011 | arg1Of | A,buffer |
R4086 | T5012 | T5013 | arg1Of | A,( |
R4087 | T5016 | T5014 | arg1Of | pH7.9,10 mM |
R4088 | T5016 | T5015 | arg1Of | pH7.9,HEPES |
R4089 | T5016 | T5017 | arg1Of | pH7.9,"," |
R4090 | T5016 | T5020 | arg1Of | pH7.9,"," |
R4091 | T5016 | T5023 | arg1Of | pH7.9,"," |
R4092 | T5019 | T5017 | arg2Of | KCl,"," |
R4093 | T5019 | T5018 | arg1Of | KCl,10 mM |
R4094 | T5022 | T5020 | arg2Of | EDTA,"," |
R4095 | T5022 | T5021 | arg1Of | EDTA,0.1 mM |
R4096 | T5023 | T5013 | arg2Of | ",",( |
R4097 | T5023 | T5029 | arg1Of | ",","," |
R4098 | T5025 | T5023 | arg2Of | EGTA,"," |
R4099 | T5025 | T5024 | arg1Of | EGTA,0.1 mM |
R4100 | T5025 | T5026 | arg1Of | EGTA,"," |
R4101 | T5028 | T5026 | arg2Of | DTT,"," |
R4102 | T5028 | T5027 | arg1Of | DTT,1 mM |
R4103 | T5031 | T5029 | arg2Of | PMSF,"," |
R4104 | T5031 | T5030 | arg1Of | PMSF,0.5 mM |
R4105 | T5032 | T5013 | arg3Of | ),( |
R4106 | T5034 | T5033 | arg2Of | incubation,After |
R4107 | T5034 | T5035 | arg1Of | incubation,for |
R4108 | T5034 | T5037 | arg1Of | incubation,on |
R4109 | T5036 | T5035 | arg2Of | 15 min,for |
R4110 | T5038 | T5037 | arg2Of | ice,on |
R4111 | T5041 | T5040 | arg1Of | cells,swollen |
R4112 | T5041 | T5042 | arg1Of | cells,were |
R4113 | T5041 | T5043 | arg2Of | cells,pressed |
R4114 | T5041 | T5054 | arg2Of | cells,centrifuged |
R4115 | T5043 | T5045 | arg1Of | pressed,times |
R4116 | T5043 | T5046 | arg1Of | pressed,through |
R4117 | T5043 | T5053 | arg1Of | pressed,and |
R4118 | T5045 | T5044 | arg1Of | times,10 |
R4119 | T5048 | T5046 | arg2Of | syringe,through |
R4120 | T5048 | T5047 | arg1Of | syringe,a |
R4121 | T5048 | T5049 | arg1Of | syringe,with |
R4122 | T5052 | T5049 | arg2Of | needle,with |
R4123 | T5052 | T5050 | arg1Of | needle,a |
R4124 | T5052 | T5051 | arg1Of | needle,26G |
R4125 | T5053 | T5033 | arg1Of | and,After |
R4126 | T5053 | T5039 | arg1Of | and,"," |
R4127 | T5053 | T5042 | arg2Of | and,were |
R4128 | T5054 | T5053 | arg2Of | centrifuged,and |
R4129 | T5054 | T5055 | arg1Of | centrifuged,at |
R4130 | T5057 | T5055 | arg2Of | r.p.m.,at |
R4131 | T5057 | T5056 | arg1Of | r.p.m.,5000 |
R4132 | T5057 | T5058 | arg1Of | r.p.m.,for |
R4133 | T5059 | T5058 | arg2Of | 5 min,for |
R4134 | T5060 | T5061 | arg1Of | Pellets,contained |
R4135 | T5060 | T5065 | arg1Of | Pellets,were |
R4136 | T5060 | T5066 | arg2Of | Pellets,washed |
R4137 | T5060 | T5074 | arg1Of | Pellets,resuspended |
R4138 | T5061 | T5064 | arg1Of | contained,and |
R4139 | T5063 | T5061 | arg2Of | nuclei,contained |
R4140 | T5063 | T5062 | arg1Of | nuclei,the |
R4141 | T5064 | T5073 | arg1Of | and,and |
R4142 | T5066 | T5064 | arg2Of | washed,and |
R4143 | T5066 | T5065 | arg2Of | washed,were |
R4144 | T5066 | T5067 | arg1Of | washed,in |
R4145 | T5069 | T5067 | arg2Of | A,in |
R4146 | T5069 | T5068 | arg1Of | A,buffer |
R4147 | T5069 | T5070 | arg1Of | A,for |
R4148 | T5072 | T5070 | arg2Of | times,for |
R4149 | T5072 | T5071 | arg1Of | times,two |
R4150 | T5074 | T5073 | arg2Of | resuspended,and |
R4151 | T5074 | T5075 | arg1Of | resuspended,in |
R4152 | T5078 | T5075 | arg2Of | C,in |
R4153 | T5078 | T5076 | arg1Of | C,50 µl |
R4154 | T5078 | T5077 | arg1Of | C,buffer |
R4155 | T5078 | T5079 | arg1Of | C,( |
R4156 | T5082 | T5080 | arg1Of | pH,20 mM |
R4157 | T5082 | T5081 | arg1Of | pH,HEPES |
R4158 | T5082 | T5083 | arg1Of | pH,7.9 |
R4159 | T5082 | T5084 | arg1Of | pH,"," |
R4160 | T5084 | T5087 | arg1Of | ",","," |
R4161 | T5086 | T5084 | arg2Of | NaCl,"," |
R4162 | T5086 | T5085 | arg1Of | NaCl,400 mM |
R4163 | T5087 | T5090 | arg1Of | ",","," |
R4164 | T5089 | T5087 | arg2Of | EDTA,"," |
R4165 | T5089 | T5088 | arg1Of | EDTA,1 mM |
R4166 | T5090 | T5079 | arg2Of | ",",( |
R4167 | T5090 | T5095 | arg1Of | ",","," |
R4168 | T5094 | T5090 | arg2Of | DTT,"," |
R4169 | T5094 | T5091 | arg1Of | DTT,1 mM |
R4170 | T5094 | T5092 | arg1Of | DTT,EGTA |
R4171 | T5094 | T5093 | arg1Of | DTT,1 mM |
R4172 | T5097 | T5095 | arg2Of | PMSF,"," |
R4173 | T5097 | T5096 | arg1Of | PMSF,1 mM |
R4174 | T5098 | T5079 | arg3Of | ),( |
R4175 | T5100 | T5099 | arg2Of | shaking,After |
R4176 | T5100 | T5101 | arg1Of | shaking,for |
R4177 | T5100 | T5109 | arg1Of | shaking,at |
R4178 | T5102 | T5101 | arg2Of | 30 min,for |
R4179 | T5102 | T5103 | arg1Of | 30 min,at |
R4180 | T5104 | T5105 | arg1Of | 4°C,and |
R4181 | T5105 | T5103 | arg2Of | and,at |
R4182 | T5105 | T5107 | arg1Of | and,for |
R4183 | T5106 | T5105 | arg2Of | centrifugation,and |
R4184 | T5108 | T5107 | arg2Of | 10 min,for |
R4185 | T5111 | T5110 | arg1Of | 000,13 |
R4186 | T5112 | T5109 | arg2Of | r.p.m.,at |
R4187 | T5112 | T5111 | arg1Of | r.p.m.,000 |
R4188 | T5114 | T5100 | arg1Of | supernatants,shaking |
R4189 | T5114 | T5115 | arg1Of | supernatants,were |
R4190 | T5114 | T5116 | arg2Of | supernatants,used |
R4191 | T5116 | T5099 | arg1Of | used,After |
R4192 | T5116 | T5113 | arg1Of | used,"," |
R4193 | T5116 | T5115 | arg2Of | used,were |
R4194 | T5116 | T5117 | arg1Of | used,as |
R4195 | T5119 | T5117 | arg2Of | extracts,as |
R4196 | T5119 | T5118 | arg1Of | extracts,nuclear |
R4197 | T5121 | T5120 | arg1Of | probes,EMSA |
R4198 | T5121 | T5122 | arg1Of | probes,were |
R4199 | T5121 | T5123 | arg2Of | probes,generated |
R4200 | T5123 | T5122 | arg2Of | generated,were |
R4201 | T5123 | T5124 | arg1Of | generated,by |
R4202 | T5123 | T5130 | arg1Of | generated,: |
R4203 | T5123 | T5131 | arg1Of | generated,APO-Sp1/3 |
R4204 | T5123 | T5145 | arg1Of | generated,according |
R4205 | T5125 | T5124 | arg2Of | annealing,by |
R4206 | T5129 | T5125 | arg2Of | oligonucleotides,annealing |
R4207 | T5129 | T5126 | arg1Of | oligonucleotides,the |
R4208 | T5129 | T5127 | arg1Of | oligonucleotides,following |
R4209 | T5129 | T5128 | arg1Of | oligonucleotides,complementary |
R4210 | T5131 | T5132 | arg1Of | APO-Sp1/3,"," |
R4211 | T5131 | T5139 | arg1Of | APO-Sp1/3,"," |
R4212 | T5134 | T5133 | arg1Of | CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′,5′- |
R4213 | T5134 | T5135 | arg1Of | CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′,and |
R4214 | T5135 | T5132 | arg2Of | and,"," |
R4215 | T5136 | T5137 | arg1Of | 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′,; |
R4216 | T5137 | T5135 | arg2Of | ;,and |
R4217 | T5138 | T5137 | arg2Of | APO-Sp1/3mut,; |
R4218 | T5140 | T5141 | arg1Of | 5′-,CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′ |
R4219 | T5140 | T5142 | arg1Of | 5′-,and |
R4220 | T5143 | T5142 | arg2Of | 5′-,and |
R4221 | T5144 | T5139 | arg2Of | GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′,"," |
R4222 | T5144 | T5140 | arg1Of | GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′,5′- |
R4223 | T5144 | T5143 | arg1Of | GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′,5′- |
R4224 | T5146 | T5145 | arg2Of | to,according |
R4225 | T5148 | T5146 | arg2Of | procedures,to |
R4226 | T5148 | T5147 | arg1Of | procedures,standard |
R4227 | T5149 | T5150 | arg1Of | Nucleotides,differing |
R4228 | T5149 | T5156 | arg1Of | Nucleotides,are |
R4229 | T5149 | T5157 | arg2Of | Nucleotides,shown |
R4230 | T5150 | T5151 | arg1Of | differing,from |
R4231 | T5155 | T5151 | arg2Of | sequence,from |
R4232 | T5155 | T5152 | arg1Of | sequence,the |
R4233 | T5155 | T5153 | arg1Of | sequence,original |
R4234 | T5155 | T5154 | arg1Of | sequence,promoter |
R4235 | T5157 | T5156 | arg2Of | shown,are |
R4236 | T5157 | T5158 | arg1Of | shown,in |
R4237 | T5160 | T5158 | arg2Of | type,in |
R4238 | T5160 | T5159 | arg1Of | type,bold |
R4239 | T5163 | T5162 | arg1Of | available,commercially |
R4240 | T5165 | T5161 | arg1Of | probe,A |
R4241 | T5165 | T5163 | arg1Of | probe,available |
R4242 | T5165 | T5164 | arg1Of | probe,Sp1 |
R4243 | T5165 | T5166 | arg1Of | probe,( |
R4244 | T5165 | T5174 | arg1Of | probe,was |
R4245 | T5165 | T5175 | arg2Of | probe,purchased |
R4246 | T5165 | T5206 | arg2Of | probe,purified |
R4247 | T5167 | T5166 | arg2Of | sc-2502,( |
R4248 | T5167 | T5168 | arg1Of | sc-2502,"," |
R4249 | T5167 | T5169 | arg2Of | sc-2502,referred |
R4250 | T5169 | T5170 | arg1Of | referred,to |
R4251 | T5169 | T5171 | arg1Of | referred,as |
R4252 | T5172 | T5171 | arg2Of | Sp1cons,as |
R4253 | T5173 | T5166 | arg3Of | ),( |
R4254 | T5175 | T5176 | arg1Of | purchased,from |
R4255 | T5175 | T5186 | modOf | purchased,using |
R4256 | T5175 | T5205 | arg1Of | purchased,and |
R4257 | T5179 | T5177 | arg1Of | Biotechnology.,Santa |
R4258 | T5179 | T5178 | arg1Of | Biotechnology.,Cruz |
R4259 | T5179 | T5183 | modOf | Biotechnology.,were |
R4260 | T5182 | T5180 | arg1Of | oligonucleotides,The |
R4261 | T5182 | T5181 | arg1Of | oligonucleotides,double-stranded |
R4262 | T5182 | T5183 | arg1Of | oligonucleotides,were |
R4263 | T5182 | T5184 | arg1Of | oligonucleotides,5′ |
R4264 | T5184 | T5183 | arg2Of | 5′,were |
R4265 | T5185 | T5176 | arg2Of | end-labeled,from |
R4266 | T5185 | T5179 | arg1Of | end-labeled,Biotechnology. |
R4267 | T5189 | T5187 | arg1Of | kinase,T4 |
R4268 | T5189 | T5188 | arg1Of | kinase,polynucleotide |
R4269 | T5189 | T5190 | arg1Of | kinase,( |
R4270 | T5189 | T5195 | arg1Of | kinase,and |
R4271 | T5193 | T5190 | arg2Of | Biolabs,( |
R4272 | T5193 | T5191 | arg1Of | Biolabs,New |
R4273 | T5193 | T5192 | arg1Of | Biolabs,England |
R4274 | T5194 | T5190 | arg3Of | ),( |
R4275 | T5195 | T5186 | arg2Of | and,using |
R4276 | T5197 | T5196 | arg2Of | γ-32P,[ |
R4277 | T5198 | T5196 | arg3Of | ],[ |
R4278 | T5199 | T5195 | arg2Of | ATP,and |
R4279 | T5199 | T5196 | arg1Of | ATP,[ |
R4280 | T5199 | T5200 | arg1Of | ATP,( |
R4281 | T5201 | T5200 | arg2Of | 3000 Ci/mmol,( |
R4282 | T5201 | T5202 | arg1Of | 3000 Ci/mmol,"," |
R4283 | T5203 | T5202 | arg2Of | Amersham,"," |
R4284 | T5204 | T5200 | arg3Of | ),( |
R4285 | T5205 | T5174 | arg2Of | and,was |
R4286 | T5206 | T5205 | arg2Of | purified,and |
R4287 | T5206 | T5207 | arg1Of | purified,by |
R4288 | T5208 | T5207 | arg2Of | using,by |
R4289 | T5211 | T5208 | arg2Of | columns,using |
R4290 | T5211 | T5209 | arg1Of | columns,Nick |
R4291 | T5211 | T5210 | arg1Of | columns,G50 |
R4292 | T5211 | T5212 | arg1Of | columns,( |
R4293 | T5213 | T5212 | arg2Of | Amersham,( |
R4294 | T5214 | T5212 | arg3Of | ),( |
R4295 | T5216 | T5215 | arg2Of | EMSA,For |
R4296 | T5218 | T5219 | arg1Of | 5 µg,of |
R4297 | T5218 | T5222 | arg1Of | 5 µg,were |
R4298 | T5218 | T5223 | arg2Of | 5 µg,preincubated |
R4299 | T5221 | T5219 | arg2Of | proteins,of |
R4300 | T5221 | T5220 | arg1Of | proteins,nuclear |
R4301 | T5223 | T5215 | arg1Of | preincubated,For |
R4302 | T5223 | T5217 | arg1Of | preincubated,"," |
R4303 | T5223 | T5222 | arg2Of | preincubated,were |
R4304 | T5223 | T5224 | arg1Of | preincubated,on |
R4305 | T5225 | T5224 | arg2Of | ice,on |
R4306 | T5225 | T5226 | arg1Of | ice,with |
R4307 | T5227 | T5226 | arg2Of | 2 µg,with |
R4308 | T5227 | T5228 | arg1Of | 2 µg,of |
R4309 | T5229 | T5230 | arg1Of | poly,( |
R4310 | T5229 | T5233 | arg1Of | poly,( |
R4311 | T5229 | T5236 | arg1Of | poly,as |
R4312 | T5229 | T5240 | arg1Of | poly,and |
R4313 | T5231 | T5230 | arg2Of | dI-dC,( |
R4314 | T5232 | T5230 | arg3Of | ),( |
R4315 | T5234 | T5233 | arg2Of | Roche,( |
R4316 | T5235 | T5233 | arg3Of | ),( |
R4317 | T5239 | T5236 | arg2Of | competitor,as |
R4318 | T5239 | T5237 | arg1Of | competitor,an |
R4319 | T5239 | T5238 | arg1Of | competitor,unspecific |
R4320 | T5240 | T5228 | arg2Of | and,of |
R4321 | T5240 | T5267 | arg1Of | and,for |
R4322 | T5241 | T5240 | arg2Of | 1 µg,and |
R4323 | T5241 | T5242 | arg1Of | 1 µg,of |
R4324 | T5241 | T5246 | arg1Of | 1 µg,in |
R4325 | T5241 | T5250 | arg1Of | 1 µg,( |
R4326 | T5245 | T5242 | arg2Of | albumin,of |
R4327 | T5245 | T5243 | arg1Of | albumin,bovine |
R4328 | T5245 | T5244 | arg1Of | albumin,serum |
R4329 | T5249 | T5246 | arg2Of | buffer,in |
R4330 | T5249 | T5247 | arg1Of | buffer,band |
R4331 | T5249 | T5248 | arg1Of | buffer,shift |
R4332 | T5252 | T5251 | arg1Of | Tris,50 mM |
R4333 | T5252 | T5253 | arg1Of | Tris,"," |
R4334 | T5253 | T5256 | arg1Of | ",","," |
R4335 | T5255 | T5253 | arg2Of | KCl,"," |
R4336 | T5255 | T5254 | arg1Of | KCl,150 mM |
R4337 | T5256 | T5259 | arg1Of | ",","," |
R4338 | T5258 | T5256 | arg2Of | EDTA,"," |
R4339 | T5258 | T5257 | arg1Of | EDTA,5 mM |
R4340 | T5259 | T5262 | arg1Of | ",","," |
R4341 | T5261 | T5259 | arg2Of | dithiothreitol,"," |
R4342 | T5261 | T5260 | arg1Of | dithiothreitol,2.5 mM |
R4343 | T5262 | T5250 | arg2Of | ",",( |
R4344 | T5263 | T5264 | arg1Of | 20,% |
R4345 | T5265 | T5262 | arg2Of | Ficoll,"," |
R4346 | T5265 | T5263 | arg1Of | Ficoll,20 |
R4347 | T5266 | T5250 | arg3Of | ),( |
R4348 | T5268 | T5267 | arg2Of | 15 min,for |
R4349 | T5270 | T5269 | arg1Of | oligonucleotides,32P-labeled |
R4350 | T5270 | T5271 | arg1Of | oligonucleotides,( |
R4351 | T5270 | T5275 | arg1Of | oligonucleotides,were |
R4352 | T5270 | T5276 | arg2Of | oligonucleotides,added |
R4353 | T5270 | T5290 | arg2Of | oligonucleotides,loaded |
R4354 | T5273 | T5271 | arg2Of | 000 c.p.m.,( |
R4355 | T5273 | T5272 | arg1Of | 000 c.p.m.,50 |
R4356 | T5274 | T5271 | arg3Of | ),( |
R4357 | T5276 | T5277 | arg1Of | added,in |
R4358 | T5276 | T5289 | arg1Of | added,and |
R4359 | T5280 | T5277 | arg2Of | volume,in |
R4360 | T5280 | T5278 | arg1Of | volume,a |
R4361 | T5280 | T5279 | arg1Of | volume,total |
R4362 | T5280 | T5281 | arg1Of | volume,of |
R4363 | T5280 | T5283 | arg1Of | volume,"," |
R4364 | T5280 | T5284 | arg2Of | volume,incubated |
R4365 | T5282 | T5281 | arg2Of | 20 µl,of |
R4366 | T5284 | T5285 | arg1Of | incubated,on |
R4367 | T5286 | T5285 | arg2Of | ice,on |
R4368 | T5286 | T5287 | arg1Of | ice,for |
R4369 | T5288 | T5287 | arg2Of | 20 min,for |
R4370 | T5289 | T5275 | arg2Of | and,were |
R4371 | T5290 | T5289 | arg2Of | loaded,and |
R4372 | T5290 | T5291 | arg1Of | loaded,onto |
R4373 | T5292 | T5293 | arg1Of | 5,% |
R4374 | T5296 | T5291 | arg2Of | gels,onto |
R4375 | T5296 | T5292 | arg1Of | gels,5 |
R4376 | T5296 | T5294 | arg1Of | gels,native |
R4377 | T5296 | T5295 | arg1Of | gels,polyacrylamide |
R4378 | T5296 | T5297 | arg1Of | gels,in |
R4379 | T5299 | T5297 | arg2Of | buffer,in |
R4380 | T5299 | T5298 | arg1Of | buffer,0.5×Tris-borate-EDTA |
R4381 | T5301 | T5300 | arg2Of | fractionation,Upon |
R4382 | T5303 | T5304 | arg1Of | gels,were |
R4383 | T5303 | T5305 | arg2Of | gels,dried |
R4384 | T5303 | T5307 | arg2Of | gels,exposed |
R4385 | T5305 | T5306 | arg1Of | dried,and |
R4386 | T5306 | T5300 | arg1Of | and,Upon |
R4387 | T5306 | T5302 | arg1Of | and,"," |
R4388 | T5306 | T5304 | arg2Of | and,were |
R4389 | T5307 | T5306 | arg2Of | exposed,and |
R4390 | T5307 | T5308 | arg1Of | exposed,for |
R4391 | T5309 | T5308 | arg2Of | autoradiography,for |
R4392 | T5312 | T5310 | arg2Of | experiments,For |
R4393 | T5312 | T5311 | arg1Of | experiments,competition |
R4394 | T5314 | T5315 | arg1Of | 1-,or |
R4395 | T5317 | T5315 | arg2Of | molar,or |
R4396 | T5317 | T5316 | arg1Of | molar,30-fold |
R4397 | T5318 | T5314 | arg1Of | excess,1- |
R4398 | T5318 | T5317 | arg1Of | excess,molar |
R4399 | T5318 | T5319 | arg1Of | excess,of |
R4400 | T5318 | T5326 | arg1Of | excess,was |
R4401 | T5318 | T5327 | arg2Of | excess,added |
R4402 | T5322 | T5323 | arg1Of | APO-Sp1/3,or |
R4403 | T5324 | T5323 | arg2Of | APO-Sp1/3mut,or |
R4404 | T5325 | T5319 | arg2Of | oligonucleotides,of |
R4405 | T5325 | T5320 | arg1Of | oligonucleotides,the |
R4406 | T5325 | T5321 | arg1Of | oligonucleotides,unlabeled |
R4407 | T5325 | T5322 | arg1Of | oligonucleotides,APO-Sp1/3 |
R4408 | T5325 | T5324 | arg1Of | oligonucleotides,APO-Sp1/3mut |
R4409 | T5327 | T5310 | arg1Of | added,For |
R4410 | T5327 | T5313 | arg1Of | added,"," |
R4411 | T5327 | T5326 | arg2Of | added,was |
R4412 | T5327 | T5328 | arg1Of | added,to |
R4413 | T5331 | T5328 | arg2Of | mixture,to |
R4414 | T5331 | T5329 | arg1Of | mixture,the |
R4415 | T5331 | T5330 | arg1Of | mixture,preincubation |
R4416 | T5334 | T5332 | arg2Of | experiments,For |
R4417 | T5334 | T5333 | arg1Of | experiments,supershift |
R4418 | T5338 | T5336 | arg1Of | antibody,2 µg |
R4419 | T5338 | T5337 | arg1Of | antibody,Sp1 |
R4420 | T5338 | T5339 | arg1Of | antibody,( |
R4421 | T5338 | T5346 | arg1Of | antibody,or |
R4422 | T5340 | T5339 | arg2Of | sc-59x,( |
R4423 | T5340 | T5341 | arg1Of | sc-59x,"," |
R4424 | T5344 | T5341 | arg2Of | Biotechnology,"," |
R4425 | T5344 | T5342 | arg1Of | Biotechnology,Santa |
R4426 | T5344 | T5343 | arg1Of | Biotechnology,Cruz |
R4427 | T5345 | T5339 | arg3Of | ),( |
R4428 | T5346 | T5356 | arg1Of | or,were |
R4429 | T5346 | T5357 | arg2Of | or,added |
R4430 | T5348 | T5346 | arg2Of | antibody,or |
R4431 | T5348 | T5347 | arg1Of | antibody,Sp3 |
R4432 | T5348 | T5349 | arg1Of | antibody,( |
R4433 | T5350 | T5349 | arg2Of | sc-644x,( |
R4434 | T5350 | T5351 | arg1Of | sc-644x,"," |
R4435 | T5354 | T5351 | arg2Of | Biotechnology,"," |
R4436 | T5354 | T5352 | arg1Of | Biotechnology,Santa |
R4437 | T5354 | T5353 | arg1Of | Biotechnology,Cruz |
R4438 | T5355 | T5349 | arg3Of | ),( |
R4439 | T5357 | T5356 | arg2Of | added,were |
R4440 | T5357 | T5358 | arg1Of | added,to |
R4441 | T5357 | T5362 | arg1Of | added,and |
R4442 | T5361 | T5358 | arg2Of | mixture,to |
R4443 | T5361 | T5359 | arg1Of | mixture,the |
R4444 | T5361 | T5360 | arg1Of | mixture,preincubation |
R4445 | T5362 | T5332 | arg1Of | and,For |
R4446 | T5362 | T5335 | arg1Of | and,"," |
R4447 | T5364 | T5363 | arg1Of | time,preincubation |
R4448 | T5364 | T5365 | arg1Of | time,was |
R4449 | T5364 | T5366 | arg2Of | time,extended |
R4450 | T5364 | T5368 | arg1Of | time,30 min |
R4451 | T5366 | T5362 | arg2Of | extended,and |
R4452 | T5366 | T5365 | arg2Of | extended,was |
R4453 | T5368 | T5366 | arg3Of | 30 min,extended |
R4454 | T5368 | T5367 | arg1Of | 30 min,to |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5903 | 789-792 | Protein | denotes | Sp1 |
T5904 | 880-883 | Protein | denotes | Sp1 |
T5905 | 1111-1114 | Protein | denotes | Sp1 |
T5906 | 1263-1287 | Protein | denotes | T4 polynucleotide kinase |
T5907 | 1534-1554 | Protein | denotes | bovine serum albumin |
T5908 | 1995-1998 | Protein | denotes | Sp1 |
T5909 | 2008-2011 | Protein | denotes | Sp1 |
T5910 | 2107-2110 | Protein | denotes | Sp1 |
T5911 | 2158-2161 | Protein | denotes | Sp3 |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5912 | 785-788 | Protein | denotes | APO |
T5913 | 789-792 | Protein | denotes | Sp1 |
T5914 | 876-879 | Protein | denotes | APO |
T5915 | 880-883 | Protein | denotes | Sp1 |
T5916 | 1111-1114 | Protein | denotes | Sp1 |
T5917 | 1146-1153 | Protein | denotes | Sp1cons |
T5918 | 1263-1287 | Protein | denotes | T4 polynucleotide kinase |
T5919 | 1534-1554 | Protein | denotes | bovine serum albumin |
T5920 | 1991-2000 | Protein | denotes | APO-Sp1/3 |
T5921 | 2004-2007 | Protein | denotes | APO |
T5922 | 2008-2011 | Protein | denotes | Sp1 |
T5923 | 2012-2016 | Protein | denotes | 3mut |
T5924 | 2107-2110 | Protein | denotes | Sp1 |
T5925 | 2158-2161 | Protein | denotes | Sp3 |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4964 | 789-792 | Protein | denotes | Sp1 |
T4965 | 880-883 | Protein | denotes | Sp1 |
T4966 | 1111-1114 | Protein | denotes | Sp1 |
T4967 | 1263-1287 | Protein | denotes | T4 polynucleotide kinase |
T4968 | 1534-1554 | Protein | denotes | bovine serum albumin |
T4969 | 1995-1998 | Protein | denotes | Sp1 |
T4970 | 2008-2011 | Protein | denotes | Sp1 |
T4971 | 2107-2110 | Protein | denotes | Sp1 |
T4972 | 2158-2161 | Protein | denotes | Sp3 |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5369 | 511-513 | P0A7Z4 | denotes | pH |
T5370 | 789-792 | P08047 | denotes | Sp1 |
T5371 | 880-883 | P08047 | denotes | Sp1 |
T5372 | 1111-1114 | P08047 | denotes | Sp1 |
T5373 | 1995-1998 | P08047 | denotes | Sp1 |
T5374 | 2008-2011 | P08047 | denotes | Sp1 |
T5375 | 2107-2110 | P08047 | denotes | Sp1 |
T5376 | 2158-2161 | Q02447 | denotes | Sp3 |