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PMC:1920263 / 13045-15338 JSONTXT

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test2

Id Subject Object Predicate Lexical cue
T4954 789-792 Protein denotes Sp1
T4955 880-883 Protein denotes Sp1
T4956 1111-1114 Protein denotes Sp1
T4957 1263-1287 Protein denotes T4 polynucleotide kinase
T4958 1534-1554 Protein denotes bovine serum albumin
T4959 1995-1998 Protein denotes Sp1
T4960 2008-2011 Protein denotes Sp1
T4961 2107-2110 Protein denotes Sp1
T4962 2158-2161 Protein denotes Sp3

GO-CC

Id Subject Object Predicate Lexical cue
T5852 97-102 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5853 287-292 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5854 2111-2119 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5855 2162-2170 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5856 2111-2119 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T5857 2162-2170 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

GO-MF

Id Subject Object Predicate Lexical cue
T5850 2111-2119 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5851 2162-2170 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

UBERON-AE

Id Subject Object Predicate Lexical cue
T4963 1541-1546 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T5390 0-15 JJ denotes Electrophoretic
T5391 16-24 NN denotes mobility
T5392 25-30 NN denotes shift
T5393 31-36 NN denotes assay
T5394 37-38 -LRB- denotes (
T5395 38-42 NNP denotes EMSA
T5396 42-43 -RRB- denotes )
T5397 44-47 IN denotes For
T5398 48-59 NN denotes preparation
T5399 60-62 IN denotes of
T5400 63-70 JJ denotes nuclear
T5401 71-79 NNS denotes extracts
T5402 79-80 , denotes ,
T5403 81-82 CD denotes 5
T5404 83-84 NN denotes ×
T5405 85-88 CD denotes 106
T5406 89-91 CD denotes A3
T5407 91-94 CD denotes .01
T5408 95-96 NNP denotes T
T5409 97-102 NNS denotes cells
T5410 103-107 VBD denotes were
T5411 108-114 VBN denotes washed
T5412 115-117 IN denotes in
T5413 118-122 JJ denotes cold
T5414 123-126 NNP denotes PBS
T5415 127-130 CC denotes and
T5416 131-142 VBD denotes resuspended
T5417 143-145 IN denotes in
T5418 146-149 CD denotes 500
T5419 150-152 NN denotes µl
T5420 153-159 NN denotes buffer
T5421 160-161 DT denotes A
T5422 162-163 -LRB- denotes (
T5423 163-165 CD denotes 10
T5424 166-168 NNP denotes mM
T5425 169-174 NNP denotes HEPES
T5426 175-178 NNP denotes pH7
T5427 178-180 CD denotes .9
T5428 180-181 , denotes ,
T5429 182-184 CD denotes 10
T5430 185-187 NNP denotes mM
T5431 188-191 NNP denotes KCl
T5432 191-192 , denotes ,
T5433 193-196 CD denotes 0.1
T5434 197-199 NNP denotes mM
T5435 200-204 NNP denotes EDTA
T5436 204-205 , denotes ,
T5437 206-209 CD denotes 0.1
T5438 210-212 NNP denotes mM
T5439 213-217 NNP denotes EGTA
T5440 217-218 , denotes ,
T5441 219-220 CD denotes 1
T5442 221-223 NNP denotes mM
T5443 224-227 NNP denotes DTT
T5444 227-228 , denotes ,
T5445 229-232 CD denotes 0.5
T5446 233-235 NNP denotes mM
T5447 236-240 NNP denotes PMSF
T5448 240-241 -RRB- denotes )
T5449 241-242 . denotes .
T5450 243-248 IN denotes After
T5451 249-259 NN denotes incubation
T5452 260-263 IN denotes for
T5453 264-266 CD denotes 15
T5454 267-270 NN denotes min
T5455 271-273 IN denotes on
T5456 274-277 NN denotes ice
T5457 277-278 , denotes ,
T5458 279-286 JJ denotes swollen
T5459 287-292 NNS denotes cells
T5460 293-297 VBD denotes were
T5461 298-305 VBN denotes pressed
T5462 306-308 CD denotes 10
T5463 309-314 NNS denotes times
T5464 315-322 IN denotes through
T5465 323-324 DT denotes a
T5466 325-332 NN denotes syringe
T5467 333-337 IN denotes with
T5468 338-339 DT denotes a
T5469 340-343 JJ denotes 26G
T5470 344-350 NN denotes needle
T5471 351-354 CC denotes and
T5472 355-366 VBN denotes centrifuged
T5473 367-369 IN denotes at
T5474 370-374 CD denotes 5000
T5475 375-381 NN denotes r.p.m.
T5476 382-385 IN denotes for
T5477 386-387 CD denotes 5
T5478 388-391 NN denotes min
T5479 391-392 . denotes .
T5480 393-400 NNS denotes Pellets
T5481 401-410 VBD denotes contained
T5482 411-414 DT denotes the
T5483 415-421 NNS denotes nuclei
T5484 422-425 CC denotes and
T5485 426-430 VBD denotes were
T5486 431-437 VBN denotes washed
T5487 438-440 IN denotes in
T5488 441-447 NN denotes buffer
T5489 448-449 DT denotes A
T5490 450-453 IN denotes for
T5491 454-457 CD denotes two
T5492 458-463 NNS denotes times
T5493 464-467 CC denotes and
T5494 468-479 VBN denotes resuspended
T5495 480-482 IN denotes in
T5496 483-485 CD denotes 50
T5497 486-488 NN denotes µl
T5498 489-495 NN denotes buffer
T5499 496-497 NNP denotes C
T5500 498-499 -LRB- denotes (
T5501 499-501 CD denotes 20
T5502 502-504 NNP denotes mM
T5503 505-510 NNP denotes HEPES
T5504 511-513 NNP denotes pH
T5505 514-517 CD denotes 7.9
T5506 517-518 , denotes ,
T5507 519-522 CD denotes 400
T5508 523-525 NNP denotes mM
T5509 526-530 NNP denotes NaCl
T5510 530-531 , denotes ,
T5511 532-533 CD denotes 1
T5512 534-536 NNP denotes mM
T5513 537-541 NNP denotes EDTA
T5514 541-542 , denotes ,
T5515 543-544 CD denotes 1
T5516 545-547 NNP denotes mM
T5517 548-552 NNP denotes EGTA
T5518 553-554 CD denotes 1
T5519 555-557 NNP denotes mM
T5520 558-561 NNP denotes DTT
T5521 561-562 , denotes ,
T5522 563-564 CD denotes 1
T5523 565-567 NNP denotes mM
T5524 568-572 NNP denotes PMSF
T5525 572-573 -RRB- denotes )
T5526 573-574 . denotes .
T5527 575-580 IN denotes After
T5528 581-588 VBG denotes shaking
T5529 589-592 IN denotes for
T5530 593-595 CD denotes 30
T5531 596-599 NN denotes min
T5532 600-602 IN denotes at
T5533 603-604 CD denotes 4
T5534 604-605 CD denotes °
T5535 605-606 NNP denotes C
T5536 607-610 CC denotes and
T5537 611-625 NN denotes centrifugation
T5538 626-629 IN denotes for
T5539 630-632 CD denotes 10
T5540 633-636 NN denotes min
T5541 637-639 IN denotes at
T5542 640-642 CD denotes 13
T5543 643-646 CD denotes 000
T5544 647-653 NN denotes r.p.m.
T5545 653-654 , denotes ,
T5546 655-667 NNS denotes supernatants
T5547 668-672 VBD denotes were
T5548 673-677 VBN denotes used
T5549 678-680 IN denotes as
T5550 681-688 JJ denotes nuclear
T5551 689-697 NNS denotes extracts
T5552 697-698 . denotes .
T5553 699-703 NNP denotes EMSA
T5554 704-710 NNS denotes probes
T5555 711-715 VBD denotes were
T5556 716-725 VBN denotes generated
T5557 726-728 IN denotes by
T5558 729-738 VBG denotes annealing
T5559 739-742 DT denotes the
T5560 743-752 VBG denotes following
T5561 753-766 JJ denotes complementary
T5562 767-783 NNS denotes oligonucleotides
T5563 783-784 : denotes :
T5564 785-792 JJ denotes APO-Sp1
T5565 792-793 NN denotes /
T5566 793-794 CD denotes 3
T5567 794-795 , denotes ,
T5568 796-797 CD denotes 5
T5569 797-798 SYM denotes
T5570 798-799 : denotes -
T5571 800-832 NN denotes CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3
T5572 832-833 NN denotes
T5573 834-837 CC denotes and
T5574 838-839 CD denotes 5
T5575 839-840 SYM denotes
T5576 840-841 : denotes -
T5577 841-873 NN denotes GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3
T5578 873-874 NN denotes
T5579 874-875 : denotes ;
T5580 876-883 NNP denotes APO-Sp1
T5581 883-884 NNP denotes /
T5582 884-888 CD denotes 3mut
T5583 888-889 , denotes ,
T5584 890-891 CD denotes 5
T5585 891-892 SYM denotes
T5586 892-893 : denotes -
T5587 894-926 NN denotes CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3
T5588 926-927 NN denotes
T5589 928-931 CC denotes and
T5590 932-933 CD denotes 5
T5591 933-934 SYM denotes
T5592 934-935 : denotes -
T5593 936-968 NN denotes GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3
T5594 968-969 NN denotes
T5595 970-979 VBG denotes according
T5596 980-982 TO denotes to
T5597 983-991 JJ denotes standard
T5598 992-1002 NNS denotes procedures
T5599 1002-1003 . denotes .
T5600 1004-1015 NNS denotes Nucleotides
T5601 1016-1025 VBG denotes differing
T5602 1026-1030 IN denotes from
T5603 1031-1034 DT denotes the
T5604 1035-1043 JJ denotes original
T5605 1044-1052 NN denotes promoter
T5606 1053-1061 NN denotes sequence
T5607 1062-1065 VBP denotes are
T5608 1066-1071 VBN denotes shown
T5609 1072-1074 IN denotes in
T5610 1075-1079 JJ denotes bold
T5611 1080-1084 NN denotes type
T5612 1084-1085 . denotes .
T5613 1086-1087 DT denotes A
T5614 1088-1100 RB denotes commercially
T5615 1101-1110 JJ denotes available
T5616 1111-1114 JJ denotes Sp1
T5617 1115-1120 NN denotes probe
T5618 1121-1122 -LRB- denotes (
T5619 1122-1129 JJ denotes sc-2502
T5620 1129-1130 , denotes ,
T5621 1131-1139 VBD denotes referred
T5622 1140-1142 TO denotes to
T5623 1143-1145 IN denotes as
T5624 1146-1153 NNS denotes Sp1cons
T5625 1153-1154 -RRB- denotes )
T5626 1155-1158 VBD denotes was
T5627 1159-1168 VBN denotes purchased
T5628 1169-1173 IN denotes from
T5629 1174-1179 NNP denotes Santa
T5630 1180-1184 NNP denotes Cruz
T5631 1185-1198 NNP denotes Biotechnology
T5632 1198-1199 . denotes .
T5633 1200-1203 DT denotes The
T5634 1204-1219 JJ denotes double-stranded
T5635 1220-1236 NNS denotes oligonucleotides
T5636 1237-1241 VBD denotes were
T5637 1242-1243 CD denotes 5
T5638 1243-1244 CD denotes
T5639 1245-1256 JJ denotes end-labeled
T5640 1257-1262 VBG denotes using
T5641 1263-1265 CD denotes T4
T5642 1266-1280 NN denotes polynucleotide
T5643 1281-1287 NN denotes kinase
T5644 1288-1289 -LRB- denotes (
T5645 1289-1292 NNP denotes New
T5646 1293-1300 NNP denotes England
T5647 1301-1308 NNPS denotes Biolabs
T5648 1308-1309 -RRB- denotes )
T5649 1310-1313 CC denotes and
T5650 1314-1315 NNP denotes [
T5651 1315-1320 NNP denotes γ-32P
T5652 1320-1321 NNP denotes ]
T5653 1321-1324 NNP denotes ATP
T5654 1325-1326 -LRB- denotes (
T5655 1326-1330 CD denotes 3000
T5656 1331-1338 NNP denotes Ci/mmol
T5657 1338-1339 , denotes ,
T5658 1340-1348 NNP denotes Amersham
T5659 1348-1349 -RRB- denotes )
T5660 1350-1353 CC denotes and
T5661 1354-1362 VBN denotes purified
T5662 1363-1365 IN denotes by
T5663 1366-1371 VBG denotes using
T5664 1372-1376 NNP denotes Nick
T5665 1377-1380 NNP denotes G50
T5666 1381-1388 NNS denotes columns
T5667 1389-1390 -LRB- denotes (
T5668 1390-1398 NNP denotes Amersham
T5669 1398-1399 -RRB- denotes )
T5670 1399-1400 . denotes .
T5671 1401-1404 IN denotes For
T5672 1405-1409 NNP denotes EMSA
T5673 1409-1410 , denotes ,
T5674 1411-1412 CD denotes 5
T5675 1413-1415 NN denotes µg
T5676 1416-1418 IN denotes of
T5677 1419-1426 JJ denotes nuclear
T5678 1427-1435 NNS denotes proteins
T5679 1436-1440 VBD denotes were
T5680 1441-1453 VBN denotes preincubated
T5681 1454-1456 IN denotes on
T5682 1457-1460 NN denotes ice
T5683 1461-1465 IN denotes with
T5684 1466-1467 CD denotes 2
T5685 1468-1470 NN denotes µg
T5686 1471-1473 IN denotes of
T5687 1474-1478 NN denotes poly
T5688 1478-1479 -LRB- denotes (
T5689 1479-1484 JJ denotes dI-dC
T5690 1484-1485 -RRB- denotes )
T5691 1486-1487 -LRB- denotes (
T5692 1487-1492 NNP denotes Roche
T5693 1492-1493 -RRB- denotes )
T5694 1494-1496 IN denotes as
T5695 1497-1499 DT denotes an
T5696 1500-1510 JJ denotes unspecific
T5697 1511-1521 NN denotes competitor
T5698 1522-1525 CC denotes and
T5699 1526-1527 CD denotes 1
T5700 1528-1530 NN denotes µg
T5701 1531-1533 IN denotes of
T5702 1534-1540 JJ denotes bovine
T5703 1541-1546 NN denotes serum
T5704 1547-1554 NN denotes albumin
T5705 1555-1557 IN denotes in
T5706 1558-1562 NN denotes band
T5707 1563-1568 NN denotes shift
T5708 1569-1575 NN denotes buffer
T5709 1576-1577 -LRB- denotes (
T5710 1577-1579 CD denotes 50
T5711 1580-1582 NNP denotes mM
T5712 1583-1587 NNP denotes Tris
T5713 1587-1588 , denotes ,
T5714 1589-1592 CD denotes 150
T5715 1593-1595 NNP denotes mM
T5716 1596-1599 NNP denotes KCl
T5717 1599-1600 , denotes ,
T5718 1601-1602 CD denotes 5
T5719 1603-1605 NNP denotes mM
T5720 1606-1610 NNP denotes EDTA
T5721 1610-1611 , denotes ,
T5722 1612-1615 CD denotes 2.5
T5723 1616-1618 NNP denotes mM
T5724 1619-1633 NN denotes dithiothreitol
T5725 1633-1634 , denotes ,
T5726 1635-1637 CD denotes 20
T5727 1637-1638 NN denotes %
T5728 1639-1645 NNP denotes Ficoll
T5729 1645-1646 -RRB- denotes )
T5730 1647-1650 IN denotes for
T5731 1651-1653 CD denotes 15
T5732 1654-1657 NN denotes min
T5733 1657-1658 . denotes .
T5734 1659-1670 JJ denotes 32P-labeled
T5735 1671-1687 NNS denotes oligonucleotides
T5736 1688-1689 -LRB- denotes (
T5737 1689-1691 CD denotes 50
T5738 1692-1695 CD denotes 000
T5739 1696-1702 NN denotes c.p.m.
T5740 1702-1703 -RRB- denotes )
T5741 1704-1708 VBD denotes were
T5742 1709-1714 VBN denotes added
T5743 1715-1717 IN denotes in
T5744 1718-1719 DT denotes a
T5745 1720-1725 JJ denotes total
T5746 1726-1732 NN denotes volume
T5747 1733-1735 IN denotes of
T5748 1736-1738 CD denotes 20
T5749 1739-1741 NN denotes µl
T5750 1741-1742 , denotes ,
T5751 1743-1752 VBN denotes incubated
T5752 1753-1755 IN denotes on
T5753 1756-1759 NN denotes ice
T5754 1760-1763 IN denotes for
T5755 1764-1766 CD denotes 20
T5756 1767-1770 NN denotes min
T5757 1771-1774 CC denotes and
T5758 1775-1781 VBN denotes loaded
T5759 1782-1786 IN denotes onto
T5760 1787-1788 CD denotes 5
T5761 1788-1789 NN denotes %
T5762 1790-1796 JJ denotes native
T5763 1797-1811 NN denotes polyacrylamide
T5764 1812-1816 NNS denotes gels
T5765 1817-1819 IN denotes in
T5766 1820-1823 CD denotes 0.5
T5767 1823-1824 CD denotes ×
T5768 1824-1840 NNP denotes Tris-borate-EDTA
T5769 1841-1847 NN denotes buffer
T5770 1847-1848 . denotes .
T5771 1849-1853 IN denotes Upon
T5772 1854-1867 NN denotes fractionation
T5773 1867-1868 , denotes ,
T5774 1869-1873 NNS denotes gels
T5775 1874-1878 VBD denotes were
T5776 1879-1884 VBN denotes dried
T5777 1885-1888 CC denotes and
T5778 1889-1896 VBN denotes exposed
T5779 1897-1900 IN denotes for
T5780 1901-1916 NN denotes autoradiography
T5781 1916-1917 . denotes .
T5782 1918-1921 IN denotes For
T5783 1922-1933 NN denotes competition
T5784 1934-1945 NNS denotes experiments
T5785 1945-1946 , denotes ,
T5786 1947-1948 LS denotes 1
T5787 1948-1949 : denotes -
T5788 1950-1952 CC denotes or
T5789 1953-1960 JJ denotes 30-fold
T5790 1961-1966 NN denotes molar
T5791 1967-1973 NN denotes excess
T5792 1974-1976 IN denotes of
T5793 1977-1980 DT denotes the
T5794 1981-1990 JJ denotes unlabeled
T5795 1991-1998 JJ denotes APO-Sp1
T5796 1998-1999 NN denotes /
T5797 1999-2000 CD denotes 3
T5798 2001-2003 CC denotes or
T5799 2004-2011 JJ denotes APO-Sp1
T5800 2011-2012 NN denotes /
T5801 2012-2016 CD denotes 3mut
T5802 2017-2033 NNS denotes oligonucleotides
T5803 2034-2037 VBD denotes was
T5804 2038-2043 VBN denotes added
T5805 2044-2046 TO denotes to
T5806 2047-2050 DT denotes the
T5807 2051-2064 NN denotes preincubation
T5808 2065-2072 NN denotes mixture
T5809 2072-2073 . denotes .
T5810 2074-2077 IN denotes For
T5811 2078-2088 JJ denotes supershift
T5812 2089-2100 NNS denotes experiments
T5813 2100-2101 , denotes ,
T5814 2102-2103 CD denotes 2
T5815 2104-2106 NN denotes µg
T5816 2107-2110 JJ denotes Sp1
T5817 2111-2119 NN denotes antibody
T5818 2120-2121 -LRB- denotes (
T5819 2121-2127 JJ denotes sc-59x
T5820 2127-2128 , denotes ,
T5821 2129-2134 NNP denotes Santa
T5822 2135-2139 NNP denotes Cruz
T5823 2140-2153 NNP denotes Biotechnology
T5824 2153-2154 -RRB- denotes )
T5825 2155-2157 CC denotes or
T5826 2158-2161 JJ denotes Sp3
T5827 2162-2170 NN denotes antibody
T5828 2171-2172 -LRB- denotes (
T5829 2172-2179 JJ denotes sc-644x
T5830 2179-2180 , denotes ,
T5831 2181-2186 NNP denotes Santa
T5832 2187-2191 NNP denotes Cruz
T5833 2192-2205 NNP denotes Biotechnology
T5834 2205-2206 -RRB- denotes )
T5835 2207-2211 VBD denotes were
T5836 2212-2217 VBN denotes added
T5837 2218-2220 TO denotes to
T5838 2221-2224 DT denotes the
T5839 2225-2238 NN denotes preincubation
T5840 2239-2246 NN denotes mixture
T5841 2247-2250 CC denotes and
T5842 2251-2264 NN denotes preincubation
T5843 2265-2269 NN denotes time
T5844 2270-2273 VBD denotes was
T5845 2274-2282 VBN denotes extended
T5846 2283-2285 TO denotes to
T5847 2286-2288 CD denotes 30
T5848 2289-2292 NN denotes min
T5849 2292-2293 . denotes .
R4455 T5390 T5393 amod Electrophoretic,assay
R4456 T5391 T5392 compound mobility,shift
R4457 T5392 T5393 compound shift,assay
R4458 T5393 T5393 ROOT assay,assay
R4459 T5394 T5393 punct (,assay
R4460 T5395 T5393 appos EMSA,assay
R4461 T5396 T5393 punct ),assay
R4462 T5397 T5411 prep For,washed
R4463 T5398 T5397 pobj preparation,For
R4464 T5399 T5398 prep of,preparation
R4465 T5400 T5401 amod nuclear,extracts
R4466 T5401 T5399 pobj extracts,of
R4467 T5402 T5411 punct ",",washed
R4468 T5403 T5409 nummod 5,cells
R4469 T5404 T5406 punct ×,A3
R4470 T5405 T5404 nummod 106,×
R4471 T5406 T5409 nummod A3,cells
R4472 T5407 T5409 nummod .01,cells
R4473 T5408 T5409 compound T,cells
R4474 T5409 T5411 nsubjpass cells,washed
R4475 T5410 T5411 auxpass were,washed
R4476 T5411 T5411 ROOT washed,washed
R4477 T5412 T5411 prep in,washed
R4478 T5413 T5414 amod cold,PBS
R4479 T5414 T5412 pobj PBS,in
R4480 T5415 T5411 cc and,washed
R4481 T5416 T5411 conj resuspended,washed
R4482 T5417 T5416 prep in,resuspended
R4483 T5418 T5419 nummod 500,µl
R4484 T5419 T5420 compound µl,buffer
R4485 T5420 T5421 compound buffer,A
R4486 T5421 T5417 pobj A,in
R4487 T5422 T5421 punct (,A
R4488 T5423 T5426 nummod 10,pH7
R4489 T5424 T5426 compound mM,pH7
R4490 T5425 T5426 compound HEPES,pH7
R4491 T5426 T5421 appos pH7,A
R4492 T5427 T5426 appos .9,pH7
R4493 T5428 T5426 punct ",",pH7
R4494 T5429 T5431 nummod 10,KCl
R4495 T5430 T5431 compound mM,KCl
R4496 T5431 T5426 appos KCl,pH7
R4497 T5432 T5431 punct ",",KCl
R4498 T5433 T5435 nummod 0.1,EDTA
R4499 T5434 T5435 compound mM,EDTA
R4500 T5435 T5431 conj EDTA,KCl
R4501 T5436 T5435 punct ",",EDTA
R4502 T5437 T5439 compound 0.1,EGTA
R4503 T5438 T5439 compound mM,EGTA
R4504 T5439 T5435 conj EGTA,EDTA
R4505 T5440 T5439 punct ",",EGTA
R4506 T5441 T5443 nummod 1,DTT
R4507 T5442 T5443 compound mM,DTT
R4508 T5443 T5439 conj DTT,EGTA
R4509 T5444 T5443 punct ",",DTT
R4510 T5445 T5447 nummod 0.5,PMSF
R4511 T5446 T5447 compound mM,PMSF
R4512 T5447 T5443 appos PMSF,DTT
R4513 T5448 T5443 punct ),DTT
R4514 T5449 T5411 punct .,washed
R4515 T5450 T5461 prep After,pressed
R4516 T5451 T5450 pobj incubation,After
R4517 T5452 T5451 prep for,incubation
R4518 T5453 T5454 nummod 15,min
R4519 T5454 T5452 pobj min,for
R4520 T5455 T5454 prep on,min
R4521 T5456 T5455 pobj ice,on
R4522 T5457 T5461 punct ",",pressed
R4523 T5458 T5459 amod swollen,cells
R4524 T5459 T5461 nsubjpass cells,pressed
R4525 T5460 T5461 auxpass were,pressed
R4526 T5461 T5461 ROOT pressed,pressed
R4527 T5462 T5463 nummod 10,times
R4528 T5463 T5461 npadvmod times,pressed
R4529 T5464 T5461 prep through,pressed
R4530 T5465 T5466 det a,syringe
R4531 T5466 T5464 pobj syringe,through
R4532 T5467 T5466 prep with,syringe
R4533 T5468 T5470 det a,needle
R4534 T5469 T5470 compound 26G,needle
R4535 T5470 T5467 pobj needle,with
R4536 T5471 T5461 cc and,pressed
R4537 T5472 T5461 conj centrifuged,pressed
R4538 T5473 T5472 prep at,centrifuged
R4539 T5474 T5475 nummod 5000,r.p.m.
R4540 T5475 T5473 pobj r.p.m.,at
R4541 T5476 T5472 prep for,centrifuged
R4542 T5477 T5478 nummod 5,min
R4543 T5478 T5476 pobj min,for
R4544 T5479 T5461 punct .,pressed
R4545 T5480 T5481 nsubj Pellets,contained
R4560 T5495 T5494 prep in,resuspended
R4561 T5496 T5499 nummod 50,C
R4562 T5497 T5499 compound µl,C
R4563 T5498 T5499 compound buffer,C
R4564 T5499 T5495 pobj C,in
R4565 T5500 T5499 punct (,C
R4566 T5501 T5504 nummod 20,pH
R4567 T5502 T5504 compound mM,pH
R4568 T5503 T5504 compound HEPES,pH
R4569 T5504 T5499 appos pH,C
R4570 T5505 T5509 nummod 7.9,NaCl
R4571 T5506 T5509 punct ",",NaCl
R4572 T5507 T5509 nummod 400,NaCl
R4573 T5508 T5509 compound mM,NaCl
R4574 T5509 T5499 appos NaCl,C
R4575 T5510 T5509 punct ",",NaCl
R4576 T5511 T5513 nummod 1,EDTA
R4577 T5512 T5513 compound mM,EDTA
R4578 T5513 T5509 conj EDTA,NaCl
R4579 T5514 T5513 punct ",",EDTA
R4580 T5515 T5520 nummod 1,DTT
R4581 T5516 T5517 compound mM,EGTA
R4582 T5517 T5520 nmod EGTA,DTT
R4583 T5518 T5520 nummod 1,DTT
R4584 T5519 T5520 compound mM,DTT
R4585 T5520 T5513 conj DTT,EDTA
R4586 T5521 T5520 punct ",",DTT
R4587 T5522 T5524 nummod 1,PMSF
R4588 T5523 T5524 compound mM,PMSF
R4589 T5524 T5520 appos PMSF,DTT
R4590 T5525 T5520 punct ),DTT
R4591 T5526 T5481 punct .,contained
R4592 T5527 T5548 prep After,used
R4593 T5528 T5527 pcomp shaking,After
R4594 T5529 T5528 prep for,shaking
R4595 T5530 T5531 nummod 30,min
R4596 T5531 T5529 pobj min,for
R4597 T5532 T5528 prep at,shaking
R4598 T5533 T5534 nummod 4,°
R4599 T5534 T5532 pobj °,at
R4600 T5535 T5528 npadvmod C,shaking
R4601 T5536 T5535 cc and,C
R4602 T5537 T5535 conj centrifugation,C
R4603 T5538 T5528 prep for,shaking
R4604 T5539 T5540 nummod 10,min
R4605 T5540 T5538 pobj min,for
R4606 T5541 T5528 prep at,shaking
R4607 T5542 T5544 nummod 13,r.p.m.
R4608 T5543 T5544 nummod 000,r.p.m.
R4609 T5544 T5541 pobj r.p.m.,at
R4610 T5545 T5548 punct ",",used
R4611 T5546 T5548 nsubjpass supernatants,used
R4612 T5547 T5548 auxpass were,used
R4613 T5548 T5548 ROOT used,used
R4614 T5549 T5548 prep as,used
R4615 T5550 T5551 amod nuclear,extracts
R4616 T5551 T5549 pobj extracts,as
R4617 T5552 T5548 punct .,used
R4618 T5553 T5554 compound EMSA,probes
R4619 T5554 T5556 nsubjpass probes,generated
R4620 T5555 T5556 auxpass were,generated
R4621 T5556 T5556 ROOT generated,generated
R4622 T5557 T5556 prep by,generated
R4623 T5558 T5557 pcomp annealing,by
R4624 T5559 T5562 det the,oligonucleotides
R4625 T5560 T5562 amod following,oligonucleotides
R4626 T5561 T5562 amod complementary,oligonucleotides
R4627 T5562 T5558 dobj oligonucleotides,annealing
R4628 T5563 T5562 punct :,oligonucleotides
R4629 T5564 T5565 compound APO-Sp1,/
R4630 T5565 T5562 appos /,oligonucleotides
R4631 T5566 T5565 nummod 3,/
R4632 T5567 T5565 punct ",",/
R4633 T5568 T5572 nummod 5,′
R4634 T5569 T5571 punct ′,CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3
R4635 T5570 T5571 punct -,CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3
R4636 T5571 T5572 compound CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3,′
R4637 T5572 T5565 appos ′,/
R4638 T5573 T5572 cc and,′
R4639 T5574 T5577 nummod 5,GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3
R4640 T5575 T5577 punct ′,GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3
R4641 T5576 T5577 punct -,GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3
R4642 T5577 T5578 compound GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3,′
R4643 T5578 T5572 conj ′,′
R4644 T5579 T5565 punct ;,/
R4645 T5580 T5581 compound APO-Sp1,/
R4646 T5581 T5595 nsubj /,according
R4647 T5582 T5581 nummod 3mut,/
R4648 T5583 T5581 punct ",",/
R4649 T5584 T5588 nummod 5,′
R4650 T5585 T5587 punct ′,CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3
R4651 T5586 T5587 punct -,CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3
R4652 T5587 T5588 compound CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3,′
R4653 T5588 T5581 appos ′,/
R4654 T5589 T5588 cc and,′
R4655 T5590 T5593 nummod 5,GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3
R4656 T5591 T5593 punct ′,GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3
R4657 T5592 T5593 punct -,GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3
R4658 T5593 T5594 compound GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3,′
R4661 T5596 T5595 prep to,according
R4662 T5597 T5598 amod standard,procedures
R4663 T5598 T5596 pobj procedures,to
R4664 T5599 T5556 punct .,generated
R4665 T5600 T5608 nsubjpass Nucleotides,shown
R4666 T5601 T5600 acl differing,Nucleotides
R4667 T5602 T5601 prep from,differing
R4668 T5603 T5606 det the,sequence
R4669 T5604 T5606 amod original,sequence
R4678 T5613 T5617 det A,probe
R4679 T5614 T5615 advmod commercially,available
R4680 T5615 T5617 amod available,probe
R4681 T5616 T5617 amod Sp1,probe
R4682 T5617 T5627 nsubjpass probe,purchased
R4683 T5618 T5621 punct (,referred
R4684 T5619 T5621 nsubj sc-2502,referred
R4685 T5620 T5621 punct ",",referred
R4686 T5621 T5617 parataxis referred,probe
R4687 T5622 T5621 prep to,referred
R4688 T5623 T5621 prep as,referred
R4689 T5624 T5623 pobj Sp1cons,as
R4690 T5625 T5621 punct ),referred
R4691 T5626 T5627 auxpass was,purchased
R4692 T5627 T5627 ROOT purchased,purchased
R4693 T5628 T5627 prep from,purchased
R4694 T5629 T5630 compound Santa,Cruz
R4695 T5630 T5631 compound Cruz,Biotechnology
R4696 T5631 T5628 pobj Biotechnology,from
R4697 T5632 T5627 punct .,purchased
R4698 T5633 T5635 det The,oligonucleotides
R4699 T5634 T5635 amod double-stranded,oligonucleotides
R4700 T5635 T5636 nsubj oligonucleotides,were
R4701 T5636 T5636 ROOT were,were
R4702 T5637 T5639 nummod 5,end-labeled
R4703 T5638 T5639 nummod ′,end-labeled
R4704 T5639 T5636 acomp end-labeled,were
R4705 T5640 T5639 advcl using,end-labeled
R4706 T5641 T5643 nummod T4,kinase
R4707 T5642 T5643 compound polynucleotide,kinase
R4708 T5643 T5640 dobj kinase,using
R4709 T5644 T5647 punct (,Biolabs
R4710 T5645 T5646 compound New,England
R4711 T5646 T5647 compound England,Biolabs
R4712 T5647 T5643 appos Biolabs,kinase
R4713 T5648 T5647 punct ),Biolabs
R4714 T5649 T5643 cc and,kinase
R4715 T5650 T5653 compound [,ATP
R4716 T5651 T5653 compound γ-32P,ATP
R4717 T5652 T5653 compound ],ATP
R4718 T5653 T5643 conj ATP,kinase
R4719 T5654 T5656 punct (,Ci/mmol
R4720 T5655 T5656 nummod 3000,Ci/mmol
R4721 T5656 T5653 appos Ci/mmol,ATP
R4722 T5657 T5656 punct ",",Ci/mmol
R4723 T5658 T5656 npadvmod Amersham,Ci/mmol
R4724 T5659 T5656 punct ),Ci/mmol
R4725 T5660 T5653 cc and,ATP
R4726 T5661 T5653 conj purified,ATP
R4727 T5662 T5661 prep by,purified
R4728 T5663 T5662 pcomp using,by
R4729 T5664 T5665 compound Nick,G50
R4730 T5665 T5666 compound G50,columns
R4731 T5666 T5663 dobj columns,using
R4732 T5667 T5666 punct (,columns
R4733 T5668 T5666 appos Amersham,columns
R4734 T5669 T5666 punct ),columns
R4735 T5670 T5636 punct .,were
R4736 T5671 T5680 prep For,preincubated
R4737 T5672 T5671 pobj EMSA,For
R4738 T5673 T5680 punct ",",preincubated
R4748 T5683 T5680 prep with,preincubated
R4761 T5696 T5697 amod unspecific,competitor
R4762 T5697 T5694 pobj competitor,as
R4763 T5698 T5697 cc and,competitor
R4764 T5699 T5700 nummod 1,µg
R4765 T5700 T5697 conj µg,competitor
R4766 T5701 T5700 prep of,µg
R4767 T5702 T5703 amod bovine,serum
R4768 T5703 T5701 pobj serum,of
R4769 T5704 T5700 conj albumin,µg
R4770 T5705 T5704 prep in,albumin
R4771 T5706 T5707 compound band,shift
R4772 T5707 T5708 compound shift,buffer
R4773 T5708 T5705 pobj buffer,in
R4774 T5709 T5712 punct (,Tris
R4775 T5710 T5712 nummod 50,Tris
R4776 T5711 T5712 compound mM,Tris
R4777 T5712 T5700 appos Tris,µg
R4778 T5713 T5712 punct ",",Tris
R4779 T5714 T5716 nummod 150,KCl
R4780 T5715 T5716 compound mM,KCl
R4781 T5716 T5712 appos KCl,Tris
R4782 T5717 T5716 punct ",",KCl
R4783 T5718 T5720 nummod 5,EDTA
R4784 T5719 T5720 compound mM,EDTA
R4785 T5720 T5716 appos EDTA,KCl
R4786 T5721 T5720 punct ",",EDTA
R4787 T5722 T5724 nummod 2.5,dithiothreitol
R4788 T5723 T5724 compound mM,dithiothreitol
R4789 T5724 T5720 appos dithiothreitol,EDTA
R4790 T5725 T5724 punct ",",dithiothreitol
R4791 T5726 T5727 nummod 20,%
R4792 T5727 T5728 compound %,Ficoll
R4793 T5728 T5724 appos Ficoll,dithiothreitol
R4794 T5729 T5724 punct ),dithiothreitol
R4795 T5730 T5720 prep for,EDTA
R4796 T5731 T5732 nummod 15,min
R4797 T5732 T5730 pobj min,for
R4798 T5733 T5680 punct .,preincubated
R4799 T5734 T5735 compound 32P-labeled,oligonucleotides
R4800 T5735 T5742 nsubjpass oligonucleotides,added
R4801 T5736 T5739 punct (,c.p.m.
R4802 T5737 T5738 nummod 50,000
R4803 T5738 T5739 nummod 000,c.p.m.
R4804 T5739 T5735 appos c.p.m.,oligonucleotides
R4805 T5740 T5735 punct ),oligonucleotides
R4806 T5741 T5742 auxpass were,added
R4807 T5742 T5742 ROOT added,added
R4808 T5743 T5742 prep in,added
R4809 T5744 T5746 det a,volume
R4810 T5745 T5746 amod total,volume
R4811 T5746 T5743 pobj volume,in
R4812 T5747 T5746 prep of,volume
R4813 T5748 T5749 nummod 20,µl
R4814 T5749 T5747 pobj µl,of
R4815 T5750 T5742 punct ",",added
R4827 T5762 T5764 amod native,gels
R4828 T5763 T5764 compound polyacrylamide,gels
R4829 T5764 T5759 pobj gels,onto
R4830 T5765 T5758 prep in,loaded
R4831 T5766 T5769 nummod 0.5,buffer
R4832 T5767 T5769 nummod ×,buffer
R4833 T5768 T5769 compound Tris-borate-EDTA,buffer
R4834 T5769 T5765 pobj buffer,in
R4835 T5770 T5742 punct .,added
R4836 T5771 T5776 prep Upon,dried
R4837 T5772 T5771 pobj fractionation,Upon
R4838 T5773 T5776 punct ",",dried
R4839 T5774 T5776 nsubjpass gels,dried
R4840 T5775 T5776 auxpass were,dried
R4841 T5776 T5776 ROOT dried,dried
R4842 T5777 T5776 cc and,dried
R4843 T5778 T5776 conj exposed,dried
R4844 T5779 T5778 prep for,exposed
R4845 T5780 T5779 pobj autoradiography,for
R4846 T5781 T5776 punct .,dried
R4847 T5782 T5804 prep For,added
R4848 T5783 T5784 compound competition,experiments
R4849 T5784 T5782 pobj experiments,For
R4850 T5785 T5804 punct ",",added
R4851 T5786 T5804 meta 1,added
R4852 T5787 T5786 punct -,1
R4853 T5788 T5786 cc or,1
R4854 T5789 T5791 amod 30-fold,excess
R4855 T5790 T5791 compound molar,excess
R4856 T5791 T5804 nsubjpass excess,added
R4857 T5792 T5791 prep of,excess
R4858 T5793 T5804 det the,added
R4859 T5794 T5796 amod unlabeled,/
R4860 T5795 T5796 compound APO-Sp1,/
R4861 T5796 T5804 nsubjpass /,added
R4862 T5797 T5796 nummod 3,/
R4863 T5798 T5796 cc or,/
R4864 T5799 T5796 conj APO-Sp1,/
R4865 T5800 T5796 conj /,/
R4866 T5801 T5800 nummod 3mut,/
R4867 T5802 T5796 appos oligonucleotides,/
R4868 T5803 T5804 auxpass was,added
R4869 T5804 T5804 ROOT added,added
R4870 T5805 T5804 prep to,added
R4871 T5806 T5808 det the,mixture
R4872 T5807 T5808 compound preincubation,mixture
R4873 T5808 T5805 pobj mixture,to
R4874 T5809 T5804 punct .,added
R4875 T5810 T5836 prep For,added
R4876 T5811 T5812 amod supershift,experiments
R4877 T5812 T5810 pobj experiments,For
R4878 T5813 T5836 punct ",",added
R4879 T5814 T5815 nummod 2,µg
R4880 T5815 T5817 nmod µg,antibody
R4881 T5816 T5817 amod Sp1,antibody
R4882 T5817 T5823 nmod antibody,Biotechnology
R4883 T5818 T5823 punct (,Biotechnology
R4884 T5819 T5823 nmod sc-59x,Biotechnology
R4885 T5820 T5823 punct ",",Biotechnology
R4886 T5821 T5822 compound Santa,Cruz
R4887 T5822 T5823 compound Cruz,Biotechnology
R4888 T5823 T5836 nsubj Biotechnology,added
R4889 T5824 T5823 punct ),Biotechnology
R4890 T5825 T5823 cc or,Biotechnology
R4891 T5826 T5827 amod Sp3,antibody
R4892 T5827 T5836 nsubjpass antibody,added
R4893 T5828 T5829 punct (,sc-644x
R4894 T5829 T5827 appos sc-644x,antibody
R4895 T5830 T5829 punct ",",sc-644x
R4896 T5831 T5833 compound Santa,Biotechnology
R4897 T5832 T5833 compound Cruz,Biotechnology
R4898 T5833 T5829 appos Biotechnology,sc-644x
R4899 T5834 T5836 punct ),added
R4900 T5835 T5836 auxpass were,added
R4901 T5836 T5836 ROOT added,added
R4902 T5837 T5836 prep to,added
R4903 T5838 T5840 det the,mixture
R4904 T5839 T5840 compound preincubation,mixture
R4905 T5840 T5843 nmod mixture,time
R4906 T5841 T5840 cc and,mixture
R4907 T5842 T5843 compound preincubation,time
R4908 T5843 T5837 pobj time,to
R4909 T5844 T5845 auxpass was,extended
R4910 T5845 T5836 conj extended,added
R4911 T5846 T5845 prep to,extended
R4912 T5847 T5848 nummod 30,min
R4913 T5848 T5846 pobj min,to
R4914 T5849 T5836 punct .,added
R4546 T5481 T5481 ROOT contained,contained
R4547 T5482 T5483 det the,nuclei
R4548 T5483 T5481 dobj nuclei,contained
R4549 T5484 T5481 cc and,contained
R4550 T5485 T5486 auxpass were,washed
R4551 T5486 T5481 conj washed,contained
R4552 T5487 T5486 prep in,washed
R4553 T5488 T5489 compound buffer,A
R4554 T5489 T5487 pobj A,in
R4555 T5490 T5486 prep for,washed
R4556 T5491 T5492 nummod two,times
R4557 T5492 T5490 pobj times,for
R4558 T5493 T5486 cc and,washed
R4559 T5494 T5486 conj resuspended,washed
R4659 T5594 T5588 conj ′,′
R4660 T5595 T5556 prep according,generated
R4670 T5605 T5606 compound promoter,sequence
R4671 T5606 T5602 pobj sequence,from
R4672 T5607 T5608 auxpass are,shown
R4673 T5608 T5608 ROOT shown,shown
R4674 T5609 T5608 prep in,shown
R4675 T5610 T5611 amod bold,type
R4676 T5611 T5609 pobj type,in
R4677 T5612 T5608 punct .,shown
R4739 T5674 T5675 nummod 5,µg
R4740 T5675 T5680 nsubjpass µg,preincubated
R4741 T5676 T5675 prep of,µg
R4742 T5677 T5678 amod nuclear,proteins
R4743 T5678 T5676 pobj proteins,of
R4744 T5679 T5680 auxpass were,preincubated
R4745 T5680 T5680 ROOT preincubated,preincubated
R4746 T5681 T5680 prep on,preincubated
R4747 T5682 T5681 pobj ice,on
R4749 T5684 T5685 nummod 2,µg
R4750 T5685 T5683 pobj µg,with
R4751 T5686 T5685 prep of,µg
R4752 T5687 T5686 pobj poly,of
R4753 T5688 T5689 punct (,dI-dC
R4754 T5689 T5687 appos dI-dC,poly
R4755 T5690 T5680 punct ),preincubated
R4756 T5691 T5692 punct (,Roche
R4757 T5692 T5680 appos Roche,preincubated
R4758 T5693 T5692 punct ),Roche
R4759 T5694 T5680 prep as,preincubated
R4760 T5695 T5697 det an,competitor
R4816 T5751 T5742 advcl incubated,added
R4817 T5752 T5751 prep on,incubated
R4818 T5753 T5752 pobj ice,on
R4819 T5754 T5751 prep for,incubated
R4820 T5755 T5756 nummod 20,min
R4821 T5756 T5754 pobj min,for
R4822 T5757 T5756 cc and,min
R4823 T5758 T5742 conj loaded,added
R4824 T5759 T5758 prep onto,loaded
R4825 T5760 T5761 nummod 5,%
R4826 T5761 T5764 nmod %,gels

sentences

Id Subject Object Predicate Lexical cue
T4973 0-43 Sentence denotes Electrophoretic mobility shift assay (EMSA)
T4974 44-242 Sentence denotes For preparation of nuclear extracts, 5 × 106 A3.01 T cells were washed in cold PBS and resuspended in 500 µl buffer A (10 mM HEPES pH7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM PMSF).
T4975 243-392 Sentence denotes After incubation for 15 min on ice, swollen cells were pressed 10 times through a syringe with a 26G needle and centrifuged at 5000 r.p.m. for 5 min.
T4976 393-574 Sentence denotes Pellets contained the nuclei and were washed in buffer A for two times and resuspended in 50 µl buffer C (20 mM HEPES pH 7.9, 400 mM NaCl, 1 mM EDTA, 1 mM EGTA 1 mM DTT, 1 mM PMSF).
T4977 575-698 Sentence denotes After shaking for 30 min at 4°C and centrifugation for 10 min at 13 000 r.p.m., supernatants were used as nuclear extracts.
T4978 699-1003 Sentence denotes EMSA probes were generated by annealing the following complementary oligonucleotides: APO-Sp1/3, 5′- CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′ and 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′; APO-Sp1/3mut, 5′- CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′ and 5′- GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′ according to standard procedures.
T4979 1004-1085 Sentence denotes Nucleotides differing from the original promoter sequence are shown in bold type.
T4980 1086-1400 Sentence denotes A commercially available Sp1 probe (sc-2502, referred to as Sp1cons) was purchased from Santa Cruz Biotechnology. The double-stranded oligonucleotides were 5′ end-labeled using T4 polynucleotide kinase (New England Biolabs) and [γ-32P]ATP (3000 Ci/mmol, Amersham) and purified by using Nick G50 columns (Amersham).
T4981 1401-1658 Sentence denotes For EMSA, 5 µg of nuclear proteins were preincubated on ice with 2 µg of poly(dI-dC) (Roche) as an unspecific competitor and 1 µg of bovine serum albumin in band shift buffer (50 mM Tris, 150 mM KCl, 5 mM EDTA, 2.5 mM dithiothreitol, 20% Ficoll) for 15 min.
T4982 1659-1848 Sentence denotes 32P-labeled oligonucleotides (50 000 c.p.m.) were added in a total volume of 20 µl, incubated on ice for 20 min and loaded onto 5% native polyacrylamide gels in 0.5×Tris-borate-EDTA buffer.
T4983 1849-1917 Sentence denotes Upon fractionation, gels were dried and exposed for autoradiography.
T4984 1918-2073 Sentence denotes For competition experiments, 1- or 30-fold molar excess of the unlabeled APO-Sp1/3 or APO-Sp1/3mut oligonucleotides was added to the preincubation mixture.
T4985 2074-2293 Sentence denotes For supershift experiments, 2 µg Sp1 antibody (sc-59x, Santa Cruz Biotechnology) or Sp3 antibody (sc-644x, Santa Cruz Biotechnology) were added to the preincubation mixture and preincubation time was extended to 30 min.
T85 0-43 Sentence denotes Electrophoretic mobility shift assay (EMSA)
T86 44-242 Sentence denotes For preparation of nuclear extracts, 5 × 106 A3.01 T cells were washed in cold PBS and resuspended in 500 µl buffer A (10 mM HEPES pH7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM PMSF).
T87 243-392 Sentence denotes After incubation for 15 min on ice, swollen cells were pressed 10 times through a syringe with a 26G needle and centrifuged at 5000 r.p.m. for 5 min.
T88 393-574 Sentence denotes Pellets contained the nuclei and were washed in buffer A for two times and resuspended in 50 µl buffer C (20 mM HEPES pH 7.9, 400 mM NaCl, 1 mM EDTA, 1 mM EGTA 1 mM DTT, 1 mM PMSF).
T89 575-698 Sentence denotes After shaking for 30 min at 4°C and centrifugation for 10 min at 13 000 r.p.m., supernatants were used as nuclear extracts.
T90 699-1003 Sentence denotes EMSA probes were generated by annealing the following complementary oligonucleotides: APO-Sp1/3, 5′- CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′ and 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′; APO-Sp1/3mut, 5′- CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′ and 5′- GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′ according to standard procedures.
T91 1004-1085 Sentence denotes Nucleotides differing from the original promoter sequence are shown in bold type.
T92 1086-1199 Sentence denotes A commercially available Sp1 probe (sc-2502, referred to as Sp1cons) was purchased from Santa Cruz Biotechnology.
T93 1200-1400 Sentence denotes The double-stranded oligonucleotides were 5′ end-labeled using T4 polynucleotide kinase (New England Biolabs) and [γ-32P]ATP (3000 Ci/mmol, Amersham) and purified by using Nick G50 columns (Amersham).
T94 1401-1658 Sentence denotes For EMSA, 5 µg of nuclear proteins were preincubated on ice with 2 µg of poly(dI-dC) (Roche) as an unspecific competitor and 1 µg of bovine serum albumin in band shift buffer (50 mM Tris, 150 mM KCl, 5 mM EDTA, 2.5 mM dithiothreitol, 20% Ficoll) for 15 min.
T95 1659-1848 Sentence denotes 32P-labeled oligonucleotides (50 000 c.p.m.) were added in a total volume of 20 µl, incubated on ice for 20 min and loaded onto 5% native polyacrylamide gels in 0.5×Tris-borate-EDTA buffer.
T96 1849-1917 Sentence denotes Upon fractionation, gels were dried and exposed for autoradiography.
T97 1918-2073 Sentence denotes For competition experiments, 1- or 30-fold molar excess of the unlabeled APO-Sp1/3 or APO-Sp1/3mut oligonucleotides was added to the preincubation mixture.
T98 2074-2293 Sentence denotes For supershift experiments, 2 µg Sp1 antibody (sc-59x, Santa Cruz Biotechnology) or Sp3 antibody (sc-644x, Santa Cruz Biotechnology) were added to the preincubation mixture and preincubation time was extended to 30 min.

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4986 0-15 JJ denotes Electrophoretic
T4987 16-24 NN denotes mobility
T4988 25-30 NN denotes shift
T4989 31-36 NN denotes assay
T4990 37-38 -LRB- denotes (
T4991 38-42 NN denotes EMSA
T4992 42-43 -RRB- denotes )
T4993 44-47 IN denotes For
T4994 48-59 NN denotes preparation
T4995 60-62 IN denotes of
T4996 63-70 JJ denotes nuclear
T4997 71-79 NN denotes extracts
T4998 79-80 -COMMA- denotes ,
T4999 81-88 CD denotes 5 × 106
T5000 89-96 NN denotes A3.01 T
T5001 97-102 NN denotes cells
T5002 103-107 VB denotes were
T5003 108-114 VB denotes washed
T5004 115-117 IN denotes in
T5005 118-122 JJ denotes cold
T5006 123-126 NN denotes PBS
T5007 127-130 CC denotes and
T5008 131-142 VB denotes resuspended
T5009 143-145 IN denotes in
T5010 146-152 CD denotes 500 µl
T5011 153-159 NN denotes buffer
T5012 160-161 NN denotes A
T5013 162-163 -LRB- denotes (
T5014 163-168 NN denotes 10 mM
T5015 169-174 NN denotes HEPES
T5016 175-180 NN denotes pH7.9
T5017 180-181 -COMMA- denotes ,
T5018 182-187 NN denotes 10 mM
T5019 188-191 NN denotes KCl
T5020 191-192 -COMMA- denotes ,
T5021 193-199 NNP denotes 0.1 mM
T5022 200-204 NNP denotes EDTA
T5023 204-205 -COMMA- denotes ,
T5024 206-212 NNP denotes 0.1 mM
T5025 213-217 NNP denotes EGTA
T5026 217-218 -COMMA- denotes ,
T5027 219-223 NN denotes 1 mM
T5028 224-227 NN denotes DTT
T5029 227-228 -COMMA- denotes ,
T5030 229-235 NN denotes 0.5 mM
T5031 236-240 NN denotes PMSF
T5032 240-241 -RRB- denotes )
T5033 243-248 IN denotes After
T5034 249-259 NN denotes incubation
T5035 260-263 IN denotes for
T5036 264-270 NN denotes 15 min
T5037 271-273 IN denotes on
T5038 274-277 NN denotes ice
T5039 277-278 -COMMA- denotes ,
T5040 279-286 NN denotes swollen
T5041 287-292 NN denotes cells
T5042 293-297 VB denotes were
T5043 298-305 VB denotes pressed
T5044 306-308 CD denotes 10
T5045 309-314 NN denotes times
T5046 315-322 IN denotes through
T5047 323-324 DT denotes a
T5048 325-332 NN denotes syringe
T5049 333-337 IN denotes with
T5050 338-339 DT denotes a
T5051 340-343 NN denotes 26G
T5052 344-350 NN denotes needle
T5053 351-354 CC denotes and
T5054 355-366 VB denotes centrifuged
T5055 367-369 IN denotes at
T5056 370-374 CD denotes 5000
T5057 375-381 NN denotes r.p.m.
T5058 382-385 IN denotes for
T5059 386-391 NN denotes 5 min
T5060 393-400 NN denotes Pellets
T5061 401-410 VB denotes contained
T5062 411-414 DT denotes the
T5063 415-421 NN denotes nuclei
T5064 422-425 CC denotes and
T5065 426-430 VB denotes were
T5066 431-437 VB denotes washed
T5067 438-440 IN denotes in
T5068 441-447 NN denotes buffer
T5069 448-449 NN denotes A
T5070 450-453 IN denotes for
T5071 454-457 CD denotes two
T5072 458-463 NN denotes times
T5073 464-467 CC denotes and
T5074 468-479 VB denotes resuspended
T5075 480-482 IN denotes in
T5076 483-488 CD denotes 50 µl
T5077 489-495 NN denotes buffer
T5078 496-497 NN denotes C
T5079 498-499 -LRB- denotes (
T5080 499-504 NN denotes 20 mM
T5081 505-510 NN denotes HEPES
T5082 511-513 NN denotes pH
T5083 514-517 CD denotes 7.9
T5084 517-518 -COMMA- denotes ,
T5085 519-525 NN denotes 400 mM
T5086 526-530 NN denotes NaCl
T5087 530-531 -COMMA- denotes ,
T5088 532-536 NN denotes 1 mM
T5089 537-541 NN denotes EDTA
T5090 541-542 -COMMA- denotes ,
T5091 543-547 NN denotes 1 mM
T5092 548-552 NN denotes EGTA
T5093 553-557 NN denotes 1 mM
T5094 558-561 NN denotes DTT
T5095 561-562 -COMMA- denotes ,
T5096 563-567 NN denotes 1 mM
T5097 568-572 NN denotes PMSF
T5098 572-573 -RRB- denotes )
T5099 575-580 IN denotes After
T5100 581-588 VB denotes shaking
T5101 589-592 IN denotes for
T5102 593-599 NN denotes 30 min
T5103 600-602 IN denotes at
T5104 603-606 NN denotes 4°C
T5105 607-610 CC denotes and
T5106 611-625 NN denotes centrifugation
T5107 626-629 IN denotes for
T5108 630-636 NN denotes 10 min
T5109 637-639 IN denotes at
T5110 640-642 CD denotes 13
T5111 643-646 CD denotes 000
T5112 647-653 NN denotes r.p.m.
T5113 653-654 -COMMA- denotes ,
T5114 655-667 NN denotes supernatants
T5115 668-672 VB denotes were
T5116 673-677 VB denotes used
T5117 678-680 IN denotes as
T5118 681-688 JJ denotes nuclear
T5119 689-697 NN denotes extracts
T5120 699-703 NN denotes EMSA
T5121 704-710 NN denotes probes
T5122 711-715 VB denotes were
T5123 716-725 VB denotes generated
T5124 726-728 IN denotes by
T5125 729-738 VB denotes annealing
T5126 739-742 DT denotes the
T5127 743-752 VB denotes following
T5128 753-766 JJ denotes complementary
T5129 767-783 NN denotes oligonucleotides
T5130 783-784 -COLON- denotes :
T5131 785-794 NN denotes APO-Sp1/3
T5132 794-795 -COMMA- denotes ,
T5133 796-799 JJ denotes 5′-
T5134 800-833 NN denotes CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′
T5135 834-837 CC denotes and
T5136 838-874 NN denotes 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′
T5137 874-875 -COLON- denotes ;
T5138 876-888 NN denotes APO-Sp1/3mut
T5139 888-889 -COMMA- denotes ,
T5140 890-893 JJ denotes 5′-
T5141 894-927 NN denotes CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′
T5142 928-931 CC denotes and
T5143 932-935 CD denotes 5′-
T5144 936-969 NN denotes GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′
T5145 970-979 VB denotes according
T5146 980-982 TO denotes to
T5147 983-991 JJ denotes standard
T5148 992-1002 NN denotes procedures
T5149 1004-1015 NN denotes Nucleotides
T5150 1016-1025 VB denotes differing
T5151 1026-1030 IN denotes from
T5152 1031-1034 DT denotes the
T5153 1035-1043 JJ denotes original
T5154 1044-1052 NN denotes promoter
T5155 1053-1061 NN denotes sequence
T5156 1062-1065 VB denotes are
T5157 1066-1071 VB denotes shown
T5158 1072-1074 IN denotes in
T5159 1075-1079 JJ denotes bold
T5160 1080-1084 NN denotes type
T5161 1086-1087 DT denotes A
T5162 1088-1100 RB denotes commercially
T5163 1101-1110 JJ denotes available
T5164 1111-1114 NN denotes Sp1
T5165 1115-1120 NN denotes probe
T5166 1121-1122 -LRB- denotes (
T5167 1122-1129 NN denotes sc-2502
T5168 1129-1130 -COMMA- denotes ,
T5169 1131-1139 VB denotes referred
T5170 1140-1142 TO denotes to
T5171 1143-1145 IN denotes as
T5172 1146-1153 NN denotes Sp1cons
T5173 1153-1154 -RRB- denotes )
T5174 1155-1158 VB denotes was
T5175 1159-1168 VB denotes purchased
T5176 1169-1173 IN denotes from
T5177 1174-1179 NNP denotes Santa
T5178 1180-1184 NNP denotes Cruz
T5179 1185-1199 NNP denotes Biotechnology.
T5180 1200-1203 DT denotes The
T5181 1204-1219 JJ denotes double-stranded
T5182 1220-1236 NN denotes oligonucleotides
T5183 1237-1241 VB denotes were
T5184 1242-1244 CD denotes 5′
T5185 1245-1256 VB denotes end-labeled
T5186 1257-1262 VB denotes using
T5187 1263-1265 NN denotes T4
T5188 1266-1280 NN denotes polynucleotide
T5189 1281-1287 NN denotes kinase
T5190 1288-1289 -LRB- denotes (
T5191 1289-1292 NNP denotes New
T5192 1293-1300 NNP denotes England
T5193 1301-1308 NNP denotes Biolabs
T5194 1308-1309 -RRB- denotes )
T5195 1310-1313 CC denotes and
T5196 1314-1315 -LRB- denotes [
T5197 1315-1320 NN denotes γ-32P
T5198 1320-1321 -RRB- denotes ]
T5199 1321-1324 NN denotes ATP
T5200 1325-1326 -LRB- denotes (
T5201 1326-1338 NN denotes 3000 Ci/mmol
T5202 1338-1339 -COMMA- denotes ,
T5203 1340-1348 NNP denotes Amersham
T5204 1348-1349 -RRB- denotes )
T5205 1350-1353 CC denotes and
T5206 1354-1362 VB denotes purified
T5207 1363-1365 IN denotes by
T5208 1366-1371 VB denotes using
T5209 1372-1376 NNP denotes Nick
T5210 1377-1380 NN denotes G50
T5211 1381-1388 NN denotes columns
T5212 1389-1390 -LRB- denotes (
T5213 1390-1398 NNP denotes Amersham
T5214 1398-1399 -RRB- denotes )
T5215 1401-1404 IN denotes For
T5216 1405-1409 NN denotes EMSA
T5217 1409-1410 -COMMA- denotes ,
T5218 1411-1415 NN denotes 5 µg
T5219 1416-1418 IN denotes of
T5220 1419-1426 JJ denotes nuclear
T5221 1427-1435 NN denotes proteins
T5222 1436-1440 VB denotes were
T5223 1441-1453 VB denotes preincubated
T5224 1454-1456 IN denotes on
T5225 1457-1460 NN denotes ice
T5226 1461-1465 IN denotes with
T5227 1466-1470 NN denotes 2 µg
T5228 1471-1473 IN denotes of
T5229 1474-1478 NN denotes poly
T5230 1478-1479 -LRB- denotes (
T5231 1479-1484 NN denotes dI-dC
T5232 1484-1485 -RRB- denotes )
T5233 1486-1487 -LRB- denotes (
T5234 1487-1492 NNP denotes Roche
T5235 1492-1493 -RRB- denotes )
T5236 1494-1496 IN denotes as
T5237 1497-1499 DT denotes an
T5238 1500-1510 JJ denotes unspecific
T5239 1511-1521 NN denotes competitor
T5240 1522-1525 CC denotes and
T5241 1526-1530 NN denotes 1 µg
T5242 1531-1533 IN denotes of
T5243 1534-1540 JJ denotes bovine
T5244 1541-1546 NN denotes serum
T5245 1547-1554 NN denotes albumin
T5246 1555-1557 IN denotes in
T5247 1558-1562 NN denotes band
T5248 1563-1568 NN denotes shift
T5249 1569-1575 NN denotes buffer
T5250 1576-1577 -LRB- denotes (
T5251 1577-1582 NNP denotes 50 mM
T5252 1583-1587 NNP denotes Tris
T5253 1587-1588 -COMMA- denotes ,
T5254 1589-1595 NN denotes 150 mM
T5255 1596-1599 NNP denotes KCl
T5256 1599-1600 -COMMA- denotes ,
T5257 1601-1605 NN denotes 5 mM
T5258 1606-1610 NN denotes EDTA
T5259 1610-1611 -COMMA- denotes ,
T5260 1612-1618 NN denotes 2.5 mM
T5261 1619-1633 NN denotes dithiothreitol
T5262 1633-1634 -COMMA- denotes ,
T5263 1635-1637 CD denotes 20
T5264 1637-1638 NN denotes %
T5265 1639-1645 NN denotes Ficoll
T5266 1645-1646 -RRB- denotes )
T5267 1647-1650 IN denotes for
T5268 1651-1657 NN denotes 15 min
T5269 1659-1670 JJ denotes 32P-labeled
T5270 1671-1687 NN denotes oligonucleotides
T5271 1688-1689 -LRB- denotes (
T5272 1689-1691 CD denotes 50
T5273 1692-1702 CD denotes 000 c.p.m.
T5274 1702-1703 -RRB- denotes )
T5275 1704-1708 VB denotes were
T5276 1709-1714 VB denotes added
T5277 1715-1717 IN denotes in
T5278 1718-1719 DT denotes a
T5279 1720-1725 JJ denotes total
T5280 1726-1732 NN denotes volume
T5281 1733-1735 IN denotes of
T5282 1736-1741 NN denotes 20 µl
T5283 1741-1742 -COMMA- denotes ,
T5284 1743-1752 VB denotes incubated
T5285 1753-1755 IN denotes on
T5286 1756-1759 NN denotes ice
T5287 1760-1763 IN denotes for
T5288 1764-1770 NN denotes 20 min
T5289 1771-1774 CC denotes and
T5290 1775-1781 VB denotes loaded
T5291 1782-1786 IN denotes onto
T5292 1787-1788 CD denotes 5
T5293 1788-1789 NN denotes %
T5294 1790-1796 JJ denotes native
T5295 1797-1811 NN denotes polyacrylamide
T5296 1812-1816 NN denotes gels
T5297 1817-1819 IN denotes in
T5298 1820-1840 NN denotes 0.5×Tris-borate-EDTA
T5299 1841-1847 NN denotes buffer
T5300 1849-1853 IN denotes Upon
T5301 1854-1867 NN denotes fractionation
T5302 1867-1868 -COMMA- denotes ,
T5303 1869-1873 NN denotes gels
T5304 1874-1878 VB denotes were
T5305 1879-1884 VB denotes dried
T5306 1885-1888 CC denotes and
T5307 1889-1896 VB denotes exposed
T5308 1897-1900 IN denotes for
T5309 1901-1916 NN denotes autoradiography
T5310 1918-1921 IN denotes For
T5311 1922-1933 NN denotes competition
T5312 1934-1945 NN denotes experiments
T5313 1945-1946 -COMMA- denotes ,
T5314 1947-1949 CD denotes 1-
T5315 1950-1952 CC denotes or
T5316 1953-1960 RB denotes 30-fold
T5317 1961-1966 JJ denotes molar
T5318 1967-1973 NN denotes excess
T5319 1974-1976 IN denotes of
T5320 1977-1980 DT denotes the
T5321 1981-1990 JJ denotes unlabeled
T5322 1991-2000 NN denotes APO-Sp1/3
T5323 2001-2003 CC denotes or
T5324 2004-2016 NN denotes APO-Sp1/3mut
T5325 2017-2033 NN denotes oligonucleotides
T5326 2034-2037 VB denotes was
T5327 2038-2043 VB denotes added
T5328 2044-2046 TO denotes to
T5329 2047-2050 DT denotes the
T5330 2051-2064 NN denotes preincubation
T5331 2065-2072 NN denotes mixture
T5332 2074-2077 IN denotes For
T5333 2078-2088 NN denotes supershift
T5334 2089-2100 NN denotes experiments
T5335 2100-2101 -COMMA- denotes ,
T5336 2102-2106 NN denotes 2 µg
T5337 2107-2110 NN denotes Sp1
T5338 2111-2119 NN denotes antibody
T5339 2120-2121 -LRB- denotes (
T5340 2121-2127 NN denotes sc-59x
T5341 2127-2128 -COMMA- denotes ,
T5342 2129-2134 NNP denotes Santa
T5343 2135-2139 NNP denotes Cruz
T5344 2140-2153 NNP denotes Biotechnology
T5345 2153-2154 -RRB- denotes )
T5346 2155-2157 CC denotes or
T5347 2158-2161 NN denotes Sp3
T5348 2162-2170 NN denotes antibody
T5349 2171-2172 -LRB- denotes (
T5350 2172-2179 NN denotes sc-644x
T5351 2179-2180 -COMMA- denotes ,
T5352 2181-2186 NNP denotes Santa
T5353 2187-2191 NNP denotes Cruz
T5354 2192-2205 NNP denotes Biotechnology
T5355 2205-2206 -RRB- denotes )
T5356 2207-2211 VB denotes were
T5357 2212-2217 VB denotes added
T5358 2218-2220 TO denotes to
T5359 2221-2224 DT denotes the
T5360 2225-2238 NN denotes preincubation
T5361 2239-2246 NN denotes mixture
T5362 2247-2250 CC denotes and
T5363 2251-2264 NN denotes preincubation
T5364 2265-2269 NN denotes time
T5365 2270-2273 VB denotes was
T5366 2274-2282 VB denotes extended
T5367 2283-2285 TO denotes to
T5368 2286-2292 VB denotes 30 min
R4059 T4989 T4986 arg1Of assay,Electrophoretic
R4060 T4989 T4987 arg1Of assay,mobility
R4061 T4989 T4988 arg1Of assay,shift
R4062 T4989 T4990 arg1Of assay,(
R4063 T4991 T4990 arg2Of EMSA,(
R4064 T4992 T4990 arg3Of ),(
R4065 T4994 T4993 arg2Of preparation,For
R4066 T4994 T4995 arg1Of preparation,of
R4067 T4997 T4995 arg2Of extracts,of
R4068 T4997 T4996 arg1Of extracts,nuclear
R4069 T5001 T4999 arg1Of cells,5 × 106
R4070 T5001 T5000 arg1Of cells,A3.01 T
R4071 T5001 T5002 arg1Of cells,were
R4072 T5001 T5003 arg2Of cells,washed
R4073 T5001 T5008 arg1Of cells,resuspended
R4074 T5003 T5002 arg2Of washed,were
R4075 T5003 T5004 arg1Of washed,in
R4076 T5003 T5007 arg1Of washed,and
R4077 T5006 T5004 arg2Of PBS,in
R4078 T5006 T5005 arg1Of PBS,cold
R4079 T5007 T4993 arg1Of and,For
R4080 T5007 T4998 arg1Of and,","
R4081 T5008 T5007 arg2Of resuspended,and
R4082 T5008 T5009 arg1Of resuspended,in
R4083 T5012 T5009 arg2Of A,in
R4084 T5012 T5010 arg1Of A,500 µl
R4085 T5012 T5011 arg1Of A,buffer
R4086 T5012 T5013 arg1Of A,(
R4087 T5016 T5014 arg1Of pH7.9,10 mM
R4088 T5016 T5015 arg1Of pH7.9,HEPES
R4089 T5016 T5017 arg1Of pH7.9,","
R4090 T5016 T5020 arg1Of pH7.9,","
R4091 T5016 T5023 arg1Of pH7.9,","
R4092 T5019 T5017 arg2Of KCl,","
R4093 T5019 T5018 arg1Of KCl,10 mM
R4094 T5022 T5020 arg2Of EDTA,","
R4095 T5022 T5021 arg1Of EDTA,0.1 mM
R4096 T5023 T5013 arg2Of ",",(
R4097 T5023 T5029 arg1Of ",",","
R4098 T5025 T5023 arg2Of EGTA,","
R4099 T5025 T5024 arg1Of EGTA,0.1 mM
R4100 T5025 T5026 arg1Of EGTA,","
R4101 T5028 T5026 arg2Of DTT,","
R4102 T5028 T5027 arg1Of DTT,1 mM
R4103 T5031 T5029 arg2Of PMSF,","
R4104 T5031 T5030 arg1Of PMSF,0.5 mM
R4105 T5032 T5013 arg3Of ),(
R4106 T5034 T5033 arg2Of incubation,After
R4107 T5034 T5035 arg1Of incubation,for
R4108 T5034 T5037 arg1Of incubation,on
R4109 T5036 T5035 arg2Of 15 min,for
R4110 T5038 T5037 arg2Of ice,on
R4111 T5041 T5040 arg1Of cells,swollen
R4112 T5041 T5042 arg1Of cells,were
R4113 T5041 T5043 arg2Of cells,pressed
R4114 T5041 T5054 arg2Of cells,centrifuged
R4115 T5043 T5045 arg1Of pressed,times
R4116 T5043 T5046 arg1Of pressed,through
R4117 T5043 T5053 arg1Of pressed,and
R4118 T5045 T5044 arg1Of times,10
R4119 T5048 T5046 arg2Of syringe,through
R4120 T5048 T5047 arg1Of syringe,a
R4121 T5048 T5049 arg1Of syringe,with
R4122 T5052 T5049 arg2Of needle,with
R4123 T5052 T5050 arg1Of needle,a
R4124 T5052 T5051 arg1Of needle,26G
R4125 T5053 T5033 arg1Of and,After
R4126 T5053 T5039 arg1Of and,","
R4127 T5053 T5042 arg2Of and,were
R4128 T5054 T5053 arg2Of centrifuged,and
R4129 T5054 T5055 arg1Of centrifuged,at
R4130 T5057 T5055 arg2Of r.p.m.,at
R4131 T5057 T5056 arg1Of r.p.m.,5000
R4132 T5057 T5058 arg1Of r.p.m.,for
R4133 T5059 T5058 arg2Of 5 min,for
R4134 T5060 T5061 arg1Of Pellets,contained
R4135 T5060 T5065 arg1Of Pellets,were
R4136 T5060 T5066 arg2Of Pellets,washed
R4137 T5060 T5074 arg1Of Pellets,resuspended
R4138 T5061 T5064 arg1Of contained,and
R4139 T5063 T5061 arg2Of nuclei,contained
R4140 T5063 T5062 arg1Of nuclei,the
R4141 T5064 T5073 arg1Of and,and
R4142 T5066 T5064 arg2Of washed,and
R4143 T5066 T5065 arg2Of washed,were
R4144 T5066 T5067 arg1Of washed,in
R4145 T5069 T5067 arg2Of A,in
R4146 T5069 T5068 arg1Of A,buffer
R4147 T5069 T5070 arg1Of A,for
R4148 T5072 T5070 arg2Of times,for
R4149 T5072 T5071 arg1Of times,two
R4150 T5074 T5073 arg2Of resuspended,and
R4151 T5074 T5075 arg1Of resuspended,in
R4152 T5078 T5075 arg2Of C,in
R4153 T5078 T5076 arg1Of C,50 µl
R4154 T5078 T5077 arg1Of C,buffer
R4155 T5078 T5079 arg1Of C,(
R4156 T5082 T5080 arg1Of pH,20 mM
R4157 T5082 T5081 arg1Of pH,HEPES
R4158 T5082 T5083 arg1Of pH,7.9
R4159 T5082 T5084 arg1Of pH,","
R4160 T5084 T5087 arg1Of ",",","
R4161 T5086 T5084 arg2Of NaCl,","
R4162 T5086 T5085 arg1Of NaCl,400 mM
R4163 T5087 T5090 arg1Of ",",","
R4164 T5089 T5087 arg2Of EDTA,","
R4165 T5089 T5088 arg1Of EDTA,1 mM
R4166 T5090 T5079 arg2Of ",",(
R4167 T5090 T5095 arg1Of ",",","
R4168 T5094 T5090 arg2Of DTT,","
R4169 T5094 T5091 arg1Of DTT,1 mM
R4170 T5094 T5092 arg1Of DTT,EGTA
R4171 T5094 T5093 arg1Of DTT,1 mM
R4172 T5097 T5095 arg2Of PMSF,","
R4173 T5097 T5096 arg1Of PMSF,1 mM
R4174 T5098 T5079 arg3Of ),(
R4175 T5100 T5099 arg2Of shaking,After
R4176 T5100 T5101 arg1Of shaking,for
R4177 T5100 T5109 arg1Of shaking,at
R4178 T5102 T5101 arg2Of 30 min,for
R4179 T5102 T5103 arg1Of 30 min,at
R4180 T5104 T5105 arg1Of 4°C,and
R4181 T5105 T5103 arg2Of and,at
R4182 T5105 T5107 arg1Of and,for
R4183 T5106 T5105 arg2Of centrifugation,and
R4184 T5108 T5107 arg2Of 10 min,for
R4185 T5111 T5110 arg1Of 000,13
R4186 T5112 T5109 arg2Of r.p.m.,at
R4187 T5112 T5111 arg1Of r.p.m.,000
R4188 T5114 T5100 arg1Of supernatants,shaking
R4189 T5114 T5115 arg1Of supernatants,were
R4190 T5114 T5116 arg2Of supernatants,used
R4191 T5116 T5099 arg1Of used,After
R4192 T5116 T5113 arg1Of used,","
R4193 T5116 T5115 arg2Of used,were
R4194 T5116 T5117 arg1Of used,as
R4195 T5119 T5117 arg2Of extracts,as
R4196 T5119 T5118 arg1Of extracts,nuclear
R4197 T5121 T5120 arg1Of probes,EMSA
R4198 T5121 T5122 arg1Of probes,were
R4199 T5121 T5123 arg2Of probes,generated
R4200 T5123 T5122 arg2Of generated,were
R4201 T5123 T5124 arg1Of generated,by
R4202 T5123 T5130 arg1Of generated,:
R4203 T5123 T5131 arg1Of generated,APO-Sp1/3
R4204 T5123 T5145 arg1Of generated,according
R4205 T5125 T5124 arg2Of annealing,by
R4206 T5129 T5125 arg2Of oligonucleotides,annealing
R4207 T5129 T5126 arg1Of oligonucleotides,the
R4208 T5129 T5127 arg1Of oligonucleotides,following
R4209 T5129 T5128 arg1Of oligonucleotides,complementary
R4210 T5131 T5132 arg1Of APO-Sp1/3,","
R4211 T5131 T5139 arg1Of APO-Sp1/3,","
R4212 T5134 T5133 arg1Of CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′,5′-
R4213 T5134 T5135 arg1Of CCAGCTGGGCGGGACCACCAGGGGAGGGGC-3′,and
R4214 T5135 T5132 arg2Of and,","
R4215 T5136 T5137 arg1Of 5′-GCCCCTCCCCTGGTGGTCCCGCCCAGCTGG-3′,;
R4216 T5137 T5135 arg2Of ;,and
R4217 T5138 T5137 arg2Of APO-Sp1/3mut,;
R4218 T5140 T5141 arg1Of 5′-,CCAGCTGTTCGGGACCACCAGGGGAGGGGC-3′
R4219 T5140 T5142 arg1Of 5′-,and
R4220 T5143 T5142 arg2Of 5′-,and
R4221 T5144 T5139 arg2Of GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′,","
R4222 T5144 T5140 arg1Of GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′,5′-
R4223 T5144 T5143 arg1Of GCCCCTCCCCTGGTGGTCCCGAACAGCTGG-3′,5′-
R4224 T5146 T5145 arg2Of to,according
R4225 T5148 T5146 arg2Of procedures,to
R4226 T5148 T5147 arg1Of procedures,standard
R4227 T5149 T5150 arg1Of Nucleotides,differing
R4228 T5149 T5156 arg1Of Nucleotides,are
R4229 T5149 T5157 arg2Of Nucleotides,shown
R4230 T5150 T5151 arg1Of differing,from
R4231 T5155 T5151 arg2Of sequence,from
R4232 T5155 T5152 arg1Of sequence,the
R4233 T5155 T5153 arg1Of sequence,original
R4234 T5155 T5154 arg1Of sequence,promoter
R4235 T5157 T5156 arg2Of shown,are
R4236 T5157 T5158 arg1Of shown,in
R4237 T5160 T5158 arg2Of type,in
R4238 T5160 T5159 arg1Of type,bold
R4239 T5163 T5162 arg1Of available,commercially
R4240 T5165 T5161 arg1Of probe,A
R4241 T5165 T5163 arg1Of probe,available
R4242 T5165 T5164 arg1Of probe,Sp1
R4243 T5165 T5166 arg1Of probe,(
R4244 T5165 T5174 arg1Of probe,was
R4245 T5165 T5175 arg2Of probe,purchased
R4246 T5165 T5206 arg2Of probe,purified
R4247 T5167 T5166 arg2Of sc-2502,(
R4248 T5167 T5168 arg1Of sc-2502,","
R4249 T5167 T5169 arg2Of sc-2502,referred
R4250 T5169 T5170 arg1Of referred,to
R4251 T5169 T5171 arg1Of referred,as
R4252 T5172 T5171 arg2Of Sp1cons,as
R4253 T5173 T5166 arg3Of ),(
R4254 T5175 T5176 arg1Of purchased,from
R4255 T5175 T5186 modOf purchased,using
R4256 T5175 T5205 arg1Of purchased,and
R4257 T5179 T5177 arg1Of Biotechnology.,Santa
R4258 T5179 T5178 arg1Of Biotechnology.,Cruz
R4259 T5179 T5183 modOf Biotechnology.,were
R4260 T5182 T5180 arg1Of oligonucleotides,The
R4261 T5182 T5181 arg1Of oligonucleotides,double-stranded
R4262 T5182 T5183 arg1Of oligonucleotides,were
R4263 T5182 T5184 arg1Of oligonucleotides,5′
R4264 T5184 T5183 arg2Of 5′,were
R4265 T5185 T5176 arg2Of end-labeled,from
R4266 T5185 T5179 arg1Of end-labeled,Biotechnology.
R4267 T5189 T5187 arg1Of kinase,T4
R4268 T5189 T5188 arg1Of kinase,polynucleotide
R4269 T5189 T5190 arg1Of kinase,(
R4270 T5189 T5195 arg1Of kinase,and
R4271 T5193 T5190 arg2Of Biolabs,(
R4272 T5193 T5191 arg1Of Biolabs,New
R4273 T5193 T5192 arg1Of Biolabs,England
R4274 T5194 T5190 arg3Of ),(
R4275 T5195 T5186 arg2Of and,using
R4276 T5197 T5196 arg2Of γ-32P,[
R4277 T5198 T5196 arg3Of ],[
R4278 T5199 T5195 arg2Of ATP,and
R4279 T5199 T5196 arg1Of ATP,[
R4280 T5199 T5200 arg1Of ATP,(
R4281 T5201 T5200 arg2Of 3000 Ci/mmol,(
R4282 T5201 T5202 arg1Of 3000 Ci/mmol,","
R4283 T5203 T5202 arg2Of Amersham,","
R4284 T5204 T5200 arg3Of ),(
R4285 T5205 T5174 arg2Of and,was
R4286 T5206 T5205 arg2Of purified,and
R4287 T5206 T5207 arg1Of purified,by
R4288 T5208 T5207 arg2Of using,by
R4289 T5211 T5208 arg2Of columns,using
R4290 T5211 T5209 arg1Of columns,Nick
R4291 T5211 T5210 arg1Of columns,G50
R4292 T5211 T5212 arg1Of columns,(
R4293 T5213 T5212 arg2Of Amersham,(
R4294 T5214 T5212 arg3Of ),(
R4295 T5216 T5215 arg2Of EMSA,For
R4296 T5218 T5219 arg1Of 5 µg,of
R4297 T5218 T5222 arg1Of 5 µg,were
R4298 T5218 T5223 arg2Of 5 µg,preincubated
R4299 T5221 T5219 arg2Of proteins,of
R4300 T5221 T5220 arg1Of proteins,nuclear
R4301 T5223 T5215 arg1Of preincubated,For
R4302 T5223 T5217 arg1Of preincubated,","
R4303 T5223 T5222 arg2Of preincubated,were
R4304 T5223 T5224 arg1Of preincubated,on
R4305 T5225 T5224 arg2Of ice,on
R4306 T5225 T5226 arg1Of ice,with
R4307 T5227 T5226 arg2Of 2 µg,with
R4308 T5227 T5228 arg1Of 2 µg,of
R4309 T5229 T5230 arg1Of poly,(
R4310 T5229 T5233 arg1Of poly,(
R4311 T5229 T5236 arg1Of poly,as
R4312 T5229 T5240 arg1Of poly,and
R4313 T5231 T5230 arg2Of dI-dC,(
R4314 T5232 T5230 arg3Of ),(
R4315 T5234 T5233 arg2Of Roche,(
R4316 T5235 T5233 arg3Of ),(
R4317 T5239 T5236 arg2Of competitor,as
R4318 T5239 T5237 arg1Of competitor,an
R4319 T5239 T5238 arg1Of competitor,unspecific
R4320 T5240 T5228 arg2Of and,of
R4321 T5240 T5267 arg1Of and,for
R4322 T5241 T5240 arg2Of 1 µg,and
R4323 T5241 T5242 arg1Of 1 µg,of
R4324 T5241 T5246 arg1Of 1 µg,in
R4325 T5241 T5250 arg1Of 1 µg,(
R4326 T5245 T5242 arg2Of albumin,of
R4327 T5245 T5243 arg1Of albumin,bovine
R4328 T5245 T5244 arg1Of albumin,serum
R4329 T5249 T5246 arg2Of buffer,in
R4330 T5249 T5247 arg1Of buffer,band
R4331 T5249 T5248 arg1Of buffer,shift
R4332 T5252 T5251 arg1Of Tris,50 mM
R4333 T5252 T5253 arg1Of Tris,","
R4334 T5253 T5256 arg1Of ",",","
R4335 T5255 T5253 arg2Of KCl,","
R4336 T5255 T5254 arg1Of KCl,150 mM
R4337 T5256 T5259 arg1Of ",",","
R4338 T5258 T5256 arg2Of EDTA,","
R4339 T5258 T5257 arg1Of EDTA,5 mM
R4340 T5259 T5262 arg1Of ",",","
R4341 T5261 T5259 arg2Of dithiothreitol,","
R4342 T5261 T5260 arg1Of dithiothreitol,2.5 mM
R4343 T5262 T5250 arg2Of ",",(
R4344 T5263 T5264 arg1Of 20,%
R4345 T5265 T5262 arg2Of Ficoll,","
R4346 T5265 T5263 arg1Of Ficoll,20
R4347 T5266 T5250 arg3Of ),(
R4348 T5268 T5267 arg2Of 15 min,for
R4349 T5270 T5269 arg1Of oligonucleotides,32P-labeled
R4350 T5270 T5271 arg1Of oligonucleotides,(
R4351 T5270 T5275 arg1Of oligonucleotides,were
R4352 T5270 T5276 arg2Of oligonucleotides,added
R4353 T5270 T5290 arg2Of oligonucleotides,loaded
R4354 T5273 T5271 arg2Of 000 c.p.m.,(
R4355 T5273 T5272 arg1Of 000 c.p.m.,50
R4356 T5274 T5271 arg3Of ),(
R4357 T5276 T5277 arg1Of added,in
R4358 T5276 T5289 arg1Of added,and
R4359 T5280 T5277 arg2Of volume,in
R4360 T5280 T5278 arg1Of volume,a
R4361 T5280 T5279 arg1Of volume,total
R4362 T5280 T5281 arg1Of volume,of
R4363 T5280 T5283 arg1Of volume,","
R4364 T5280 T5284 arg2Of volume,incubated
R4365 T5282 T5281 arg2Of 20 µl,of
R4366 T5284 T5285 arg1Of incubated,on
R4367 T5286 T5285 arg2Of ice,on
R4368 T5286 T5287 arg1Of ice,for
R4369 T5288 T5287 arg2Of 20 min,for
R4370 T5289 T5275 arg2Of and,were
R4371 T5290 T5289 arg2Of loaded,and
R4372 T5290 T5291 arg1Of loaded,onto
R4373 T5292 T5293 arg1Of 5,%
R4374 T5296 T5291 arg2Of gels,onto
R4375 T5296 T5292 arg1Of gels,5
R4376 T5296 T5294 arg1Of gels,native
R4377 T5296 T5295 arg1Of gels,polyacrylamide
R4378 T5296 T5297 arg1Of gels,in
R4379 T5299 T5297 arg2Of buffer,in
R4380 T5299 T5298 arg1Of buffer,0.5×Tris-borate-EDTA
R4381 T5301 T5300 arg2Of fractionation,Upon
R4382 T5303 T5304 arg1Of gels,were
R4383 T5303 T5305 arg2Of gels,dried
R4384 T5303 T5307 arg2Of gels,exposed
R4385 T5305 T5306 arg1Of dried,and
R4386 T5306 T5300 arg1Of and,Upon
R4387 T5306 T5302 arg1Of and,","
R4388 T5306 T5304 arg2Of and,were
R4389 T5307 T5306 arg2Of exposed,and
R4390 T5307 T5308 arg1Of exposed,for
R4391 T5309 T5308 arg2Of autoradiography,for
R4392 T5312 T5310 arg2Of experiments,For
R4393 T5312 T5311 arg1Of experiments,competition
R4394 T5314 T5315 arg1Of 1-,or
R4395 T5317 T5315 arg2Of molar,or
R4396 T5317 T5316 arg1Of molar,30-fold
R4397 T5318 T5314 arg1Of excess,1-
R4398 T5318 T5317 arg1Of excess,molar
R4399 T5318 T5319 arg1Of excess,of
R4400 T5318 T5326 arg1Of excess,was
R4401 T5318 T5327 arg2Of excess,added
R4402 T5322 T5323 arg1Of APO-Sp1/3,or
R4403 T5324 T5323 arg2Of APO-Sp1/3mut,or
R4404 T5325 T5319 arg2Of oligonucleotides,of
R4405 T5325 T5320 arg1Of oligonucleotides,the
R4406 T5325 T5321 arg1Of oligonucleotides,unlabeled
R4407 T5325 T5322 arg1Of oligonucleotides,APO-Sp1/3
R4408 T5325 T5324 arg1Of oligonucleotides,APO-Sp1/3mut
R4409 T5327 T5310 arg1Of added,For
R4410 T5327 T5313 arg1Of added,","
R4411 T5327 T5326 arg2Of added,was
R4412 T5327 T5328 arg1Of added,to
R4413 T5331 T5328 arg2Of mixture,to
R4414 T5331 T5329 arg1Of mixture,the
R4415 T5331 T5330 arg1Of mixture,preincubation
R4416 T5334 T5332 arg2Of experiments,For
R4417 T5334 T5333 arg1Of experiments,supershift
R4418 T5338 T5336 arg1Of antibody,2 µg
R4419 T5338 T5337 arg1Of antibody,Sp1
R4420 T5338 T5339 arg1Of antibody,(
R4421 T5338 T5346 arg1Of antibody,or
R4422 T5340 T5339 arg2Of sc-59x,(
R4423 T5340 T5341 arg1Of sc-59x,","
R4424 T5344 T5341 arg2Of Biotechnology,","
R4425 T5344 T5342 arg1Of Biotechnology,Santa
R4426 T5344 T5343 arg1Of Biotechnology,Cruz
R4427 T5345 T5339 arg3Of ),(
R4428 T5346 T5356 arg1Of or,were
R4429 T5346 T5357 arg2Of or,added
R4430 T5348 T5346 arg2Of antibody,or
R4431 T5348 T5347 arg1Of antibody,Sp3
R4432 T5348 T5349 arg1Of antibody,(
R4433 T5350 T5349 arg2Of sc-644x,(
R4434 T5350 T5351 arg1Of sc-644x,","
R4435 T5354 T5351 arg2Of Biotechnology,","
R4436 T5354 T5352 arg1Of Biotechnology,Santa
R4437 T5354 T5353 arg1Of Biotechnology,Cruz
R4438 T5355 T5349 arg3Of ),(
R4439 T5357 T5356 arg2Of added,were
R4440 T5357 T5358 arg1Of added,to
R4441 T5357 T5362 arg1Of added,and
R4442 T5361 T5358 arg2Of mixture,to
R4443 T5361 T5359 arg1Of mixture,the
R4444 T5361 T5360 arg1Of mixture,preincubation
R4445 T5362 T5332 arg1Of and,For
R4446 T5362 T5335 arg1Of and,","
R4447 T5364 T5363 arg1Of time,preincubation
R4448 T5364 T5365 arg1Of time,was
R4449 T5364 T5366 arg2Of time,extended
R4450 T5364 T5368 arg1Of time,30 min
R4451 T5366 T5362 arg2Of extended,and
R4452 T5366 T5365 arg2Of extended,was
R4453 T5368 T5366 arg3Of 30 min,extended
R4454 T5368 T5367 arg1Of 30 min,to

events-check-again

Id Subject Object Predicate Lexical cue
T5903 789-792 Protein denotes Sp1
T5904 880-883 Protein denotes Sp1
T5905 1111-1114 Protein denotes Sp1
T5906 1263-1287 Protein denotes T4 polynucleotide kinase
T5907 1534-1554 Protein denotes bovine serum albumin
T5908 1995-1998 Protein denotes Sp1
T5909 2008-2011 Protein denotes Sp1
T5910 2107-2110 Protein denotes Sp1
T5911 2158-2161 Protein denotes Sp3

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T5912 785-788 Protein denotes APO
T5913 789-792 Protein denotes Sp1
T5914 876-879 Protein denotes APO
T5915 880-883 Protein denotes Sp1
T5916 1111-1114 Protein denotes Sp1
T5917 1146-1153 Protein denotes Sp1cons
T5918 1263-1287 Protein denotes T4 polynucleotide kinase
T5919 1534-1554 Protein denotes bovine serum albumin
T5920 1991-2000 Protein denotes APO-Sp1/3
T5921 2004-2007 Protein denotes APO
T5922 2008-2011 Protein denotes Sp1
T5923 2012-2016 Protein denotes 3mut
T5924 2107-2110 Protein denotes Sp1
T5925 2158-2161 Protein denotes Sp3

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T4964 789-792 Protein denotes Sp1
T4965 880-883 Protein denotes Sp1
T4966 1111-1114 Protein denotes Sp1
T4967 1263-1287 Protein denotes T4 polynucleotide kinase
T4968 1534-1554 Protein denotes bovine serum albumin
T4969 1995-1998 Protein denotes Sp1
T4970 2008-2011 Protein denotes Sp1
T4971 2107-2110 Protein denotes Sp1
T4972 2158-2161 Protein denotes Sp3

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T5369 511-513 P0A7Z4 denotes pH
T5370 789-792 P08047 denotes Sp1
T5371 880-883 P08047 denotes Sp1
T5372 1111-1114 P08047 denotes Sp1
T5373 1995-1998 P08047 denotes Sp1
T5374 2008-2011 P08047 denotes Sp1
T5375 2107-2110 P08047 denotes Sp1
T5376 2158-2161 Q02447 denotes Sp3