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PMC:1920263 / 10494-11164 JSONTXT

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test2

Id Subject Object Predicate Lexical cue
T3861 161-164 Protein denotes A3G

GO-CC

Id Subject Object Predicate Lexical cue
T4115 88-93 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-BP

Id Subject Object Predicate Lexical cue
T3868 130-145 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T3869 358-367 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T3983 0-1 CD denotes 5
T3984 1-2 SYM denotes ′
T3985 2-3 : denotes -
T3986 3-8 NNP denotes Rapid
T3987 9-22 NN denotes amplification
T3988 23-25 IN denotes of
T3989 26-30 NNP denotes cDNA
T3990 31-35 VBZ denotes ends
T3991 36-44 NN denotes analysis
T3992 45-46 -LRB- denotes (
T3993 46-50 NNP denotes RACE
T3994 50-51 -RRB- denotes )
T3995 52-57 JJ denotes Total
T3996 58-61 NNP denotes RNA
T3997 62-65 VBD denotes was
T3998 66-74 VBN denotes isolated
T3999 75-79 IN denotes from
T4000 80-82 CD denotes A3
T4001 82-85 CD denotes .01
T4002 86-87 NNP denotes T
T4003 88-93 NNS denotes cells
T4004 94-99 VBG denotes using
T4005 100-106 NNP denotes RNeasy
T4006 107-111 NNP denotes mini
T4007 112-115 NN denotes kit
T4008 116-117 -LRB- denotes (
T4009 117-123 NNP denotes Qiagen
T4010 123-124 -RRB- denotes )
T4011 124-125 . denotes .
T4012 126-129 DT denotes The
T4013 130-145 JJ denotes transcriptional
T4014 146-151 NN denotes start
T4015 152-157 NNS denotes sites
T4016 158-160 IN denotes of
T4017 161-164 NNP denotes A3G
T4018 165-169 VBD denotes were
T4019 170-180 VBN denotes identified
T4020 181-186 VBG denotes using
T4021 187-190 DT denotes the
T4022 191-192 CD denotes 5
T4023 192-193 NN denotes ′
T4024 193-194 NN denotes /
T4025 194-195 CD denotes 3
T4026 195-196 NN denotes ′
T4027 197-201 NNP denotes RACE
T4028 202-205 NNP denotes Kit
T4029 205-206 , denotes ,
T4030 207-210 CD denotes 2nd
T4031 211-221 NNP denotes Generation
T4032 222-223 -LRB- denotes (
T4033 223-228 NNP denotes Roche
T4034 228-229 -RRB- denotes )
T4035 230-239 VBG denotes according
T4036 240-242 TO denotes to
T4037 243-246 DT denotes the
T4038 247-259 NN denotes manufacturer
T4039 259-261 POS denotes 's
T4040 262-274 NNS denotes instructions
T4041 274-275 . denotes .
T4042 276-279 DT denotes The
T4043 280-289 VBG denotes following
T4044 290-297 NNS denotes primers
T4045 298-302 VBD denotes were
T4046 303-307 VBN denotes used
T4047 307-308 : denotes :
T4048 309-320 NN denotes RACE-APO3G1
T4049 321-322 -LRB- denotes (
T4050 322-323 CD denotes 5
T4051 323-324 SYM denotes ′
T4052 324-325 : denotes -
T4053 325-346 NN denotes TATCCCTTGTACACTTTGT-3
T4054 346-347 NN denotes ′
T4055 347-348 -RRB- denotes )
T4056 349-352 IN denotes for
T4057 353-357 NNP denotes cDNA
T4058 358-367 NN denotes synthesis
T4059 367-368 , denotes ,
T4060 369-380 NN denotes RACE-APO3G2
T4061 381-382 -LRB- denotes (
T4062 382-383 CD denotes 5
T4063 383-384 SYM denotes ′
T4064 384-385 : denotes -
T4065 385-405 NN denotes CATACTCCTGGTCACGAT-3
T4066 405-406 NN denotes ′
T4067 406-407 -RRB- denotes )
T4068 408-411 IN denotes for
T4069 412-415 DT denotes the
T4070 416-421 JJ denotes first
T4071 422-425 NNP denotes PCR
T4072 426-429 CC denotes and
T4073 430-444 NNP denotes RACE-APO3Gnest
T4074 445-446 -LRB- denotes (
T4075 446-447 CD denotes 5
T4076 447-448 SYM denotes ′
T4077 448-449 : denotes -
T4078 449-470 NN denotes GAATACACCTGGCCTCGAA-3
T4079 470-471 NN denotes ′
T4080 471-472 -RRB- denotes )
T4081 473-476 IN denotes for
T4082 477-480 DT denotes the
T4083 481-487 JJ denotes nested
T4084 488-491 NNP denotes PCR
T4085 491-492 . denotes .
T4086 493-501 NN denotes Reaction
T4087 502-510 NNS denotes products
T4088 511-515 VBD denotes were
T4089 516-524 VBN denotes analyzed
T4090 525-527 IN denotes by
T4091 528-535 JJ denotes agarose
T4092 536-539 NN denotes gel
T4093 540-555 NN denotes electrophoresis
T4094 555-556 , denotes ,
T4095 557-565 VBN denotes purified
T4096 566-571 VBG denotes using
T4097 572-580 NNP denotes QIAquick
T4098 581-584 NN denotes gel
T4099 585-595 NN denotes extraction
T4100 596-599 NN denotes kit
T4101 600-601 -LRB- denotes (
T4102 601-607 NNP denotes Qiagen
T4103 607-608 -RRB- denotes )
T4104 608-609 , denotes ,
T4105 610-620 JJ denotes T/A-cloned
T4106 621-625 IN denotes into
T4107 626-632 NN denotes vector
T4108 633-642 NN denotes pCR4-TOPO
T4109 643-644 -LRB- denotes (
T4110 644-654 NNP denotes Invitrogen
T4111 654-655 -RRB- denotes )
T4112 656-659 CC denotes and
T4113 660-669 VBD denotes sequenced
T4114 669-670 . denotes .
R3254 T3983 T3987 nummod 5,amplification
R3255 T3984 T3986 punct ′,Rapid
R3256 T3985 T3986 punct -,Rapid
R3257 T3986 T3987 compound Rapid,amplification
R3258 T3987 T3990 nsubj amplification,ends
R3259 T3988 T3987 prep of,amplification
R3260 T3989 T3988 pobj cDNA,of
R3261 T3990 T3990 ROOT ends,ends
R3262 T3991 T3990 dobj analysis,ends
R3263 T3992 T3991 punct (,analysis
R3264 T3993 T3991 appos RACE,analysis
R3265 T3994 T3991 punct ),analysis
R3266 T3995 T3996 amod Total,RNA
R3267 T3996 T3998 nsubjpass RNA,isolated
R3268 T3997 T3998 auxpass was,isolated
R3269 T3998 T3998 ROOT isolated,isolated
R3270 T3999 T3998 prep from,isolated
R3271 T4000 T4003 nummod A3,cells
R3272 T4001 T4003 nummod .01,cells
R3273 T4002 T4003 compound T,cells
R3274 T4003 T3999 pobj cells,from
R3275 T4004 T3998 advcl using,isolated
R3276 T4005 T4007 compound RNeasy,kit
R3277 T4006 T4007 compound mini,kit
R3278 T4007 T4004 dobj kit,using
R3279 T4008 T4007 punct (,kit
R3280 T4009 T4007 appos Qiagen,kit
R3281 T4010 T4007 punct ),kit
R3282 T4011 T3998 punct .,isolated
R3283 T4012 T4015 det The,sites
R3284 T4013 T4014 nsubj transcriptional,start
R3285 T4014 T4015 compound start,sites
R3286 T4015 T4019 nsubjpass sites,identified
R3287 T4016 T4015 prep of,sites
R3288 T4017 T4016 pobj A3G,of
R3289 T4018 T4019 auxpass were,identified
R3290 T4019 T4019 ROOT identified,identified
R3291 T4020 T4019 advcl using,identified
R3292 T4021 T4028 det the,Kit
R3293 T4022 T4023 nummod 5,′
R3294 T4023 T4024 compound ′,/
R3295 T4024 T4028 nmod /,Kit
R3296 T4025 T4024 nummod 3,/
R3297 T4026 T4028 compound ′,Kit
R3298 T4027 T4028 compound RACE,Kit
R3299 T4028 T4020 dobj Kit,using
R3300 T4029 T4035 punct ",",according
R3301 T4030 T4031 nummod 2nd,Generation
R3302 T4031 T4035 nsubj Generation,according
R3303 T4032 T4033 punct (,Roche
R3304 T4033 T4031 appos Roche,Generation
R3305 T4034 T4031 punct ),Generation
R3306 T4035 T4019 prep according,identified
R3307 T4036 T4035 prep to,according
R3308 T4037 T4038 det the,manufacturer
R3309 T4038 T4040 poss manufacturer,instructions
R3310 T4039 T4038 case 's,manufacturer
R3311 T4040 T4036 pobj instructions,to
R3312 T4041 T4035 punct .,according
R3313 T4042 T4044 det The,primers
R3314 T4043 T4044 amod following,primers
R3315 T4044 T4046 nsubjpass primers,used
R3316 T4045 T4046 auxpass were,used
R3317 T4046 T4048 ccomp used,RACE-APO3G1
R3318 T4047 T4048 punct :,RACE-APO3G1
R3319 T4048 T4048 ROOT RACE-APO3G1,RACE-APO3G1
R3320 T4049 T4050 punct (,5
R3321 T4050 T4054 nummod 5,′
R3322 T4051 T4050 punct ′,5
R3323 T4052 T4053 punct -,TATCCCTTGTACACTTTGT-3
R3324 T4053 T4054 compound TATCCCTTGTACACTTTGT-3,′
R3325 T4054 T4048 appos ′,RACE-APO3G1
R3326 T4055 T4048 punct ),RACE-APO3G1
R3327 T4056 T4048 prep for,RACE-APO3G1
R3328 T4057 T4058 compound cDNA,synthesis
R3329 T4058 T4056 pobj synthesis,for
R3330 T4059 T4048 punct ",",RACE-APO3G1
R3331 T4060 T4048 appos RACE-APO3G2,RACE-APO3G1
R3332 T4061 T4062 punct (,5
R3333 T4062 T4065 meta 5,CATACTCCTGGTCACGAT-3
R3334 T4063 T4062 punct ′,5
R3335 T4064 T4065 punct -,CATACTCCTGGTCACGAT-3
R3336 T4065 T4066 compound CATACTCCTGGTCACGAT-3,′
R3337 T4066 T4060 appos ′,RACE-APO3G2
R3338 T4067 T4060 punct ),RACE-APO3G2
R3339 T4068 T4048 prep for,RACE-APO3G1
R3340 T4069 T4071 det the,PCR
R3341 T4070 T4071 amod first,PCR
R3342 T4071 T4068 pobj PCR,for
R3343 T4072 T4071 cc and,PCR
R3344 T4073 T4071 conj RACE-APO3Gnest,PCR
R3345 T4074 T4075 punct (,5
R3346 T4075 T4078 acl 5,GAATACACCTGGCCTCGAA-3
R3347 T4076 T4078 punct ′,GAATACACCTGGCCTCGAA-3
R3348 T4077 T4078 punct -,GAATACACCTGGCCTCGAA-3
R3349 T4078 T4079 compound GAATACACCTGGCCTCGAA-3,′
R3350 T4079 T4071 appos ′,PCR
R3351 T4080 T4048 punct ),RACE-APO3G1
R3352 T4081 T4048 prep for,RACE-APO3G1
R3353 T4082 T4084 det the,PCR
R3354 T4083 T4084 amod nested,PCR
R3355 T4084 T4081 pobj PCR,for
R3356 T4085 T4048 punct .,RACE-APO3G1
R3357 T4086 T4087 amod Reaction,products
R3358 T4087 T4089 nsubjpass products,analyzed
R3359 T4088 T4089 auxpass were,analyzed
R3360 T4089 T4089 ROOT analyzed,analyzed
R3361 T4090 T4089 agent by,analyzed
R3362 T4091 T4093 amod agarose,electrophoresis
R3363 T4092 T4093 compound gel,electrophoresis
R3364 T4093 T4090 pobj electrophoresis,by
R3365 T4094 T4093 punct ",",electrophoresis
R3366 T4095 T4105 amod purified,T/A-cloned
R3367 T4096 T4095 xcomp using,purified
R3368 T4097 T4100 compound QIAquick,kit
R3369 T4098 T4099 compound gel,extraction
R3370 T4099 T4100 compound extraction,kit
R3371 T4100 T4096 dobj kit,using
R3372 T4101 T4100 punct (,kit
R3373 T4102 T4100 appos Qiagen,kit
R3374 T4103 T4100 punct ),kit
R3375 T4104 T4100 punct ",",kit
R3376 T4105 T4093 amod T/A-cloned,electrophoresis
R3377 T4106 T4105 prep into,T/A-cloned
R3378 T4107 T4108 compound vector,pCR4-TOPO
R3379 T4108 T4106 pobj pCR4-TOPO,into
R3380 T4109 T4110 punct (,Invitrogen
R3381 T4110 T4108 appos Invitrogen,pCR4-TOPO
R3382 T4111 T4110 punct ),Invitrogen
R3383 T4112 T4105 cc and,T/A-cloned
R3384 T4113 T4105 conj sequenced,T/A-cloned
R3385 T4114 T4089 punct .,analyzed

sentences

Id Subject Object Predicate Lexical cue
T3863 0-51 Sentence denotes 5′-Rapid amplification of cDNA ends analysis (RACE)
T3864 52-125 Sentence denotes Total RNA was isolated from A3.01 T cells using RNeasy mini kit (Qiagen).
T3865 126-275 Sentence denotes The transcriptional start sites of A3G were identified using the 5′/3′ RACE Kit, 2nd Generation (Roche) according to the manufacturer's instructions.
T3866 276-492 Sentence denotes The following primers were used: RACE-APO3G1 (5′-TATCCCTTGTACACTTTGT-3′) for cDNA synthesis, RACE-APO3G2 (5′-CATACTCCTGGTCACGAT-3′) for the first PCR and RACE-APO3Gnest (5′-GAATACACCTGGCCTCGAA-3′) for the nested PCR.
T3867 493-670 Sentence denotes Reaction products were analyzed by agarose gel electrophoresis, purified using QIAquick gel extraction kit (Qiagen), T/A-cloned into vector pCR4-TOPO (Invitrogen) and sequenced.
T68 0-51 Sentence denotes 5′-Rapid amplification of cDNA ends analysis (RACE)
T69 52-125 Sentence denotes Total RNA was isolated from A3.01 T cells using RNeasy mini kit (Qiagen).
T70 126-275 Sentence denotes The transcriptional start sites of A3G were identified using the 5′/3′ RACE Kit, 2nd Generation (Roche) according to the manufacturer's instructions.
T71 276-492 Sentence denotes The following primers were used: RACE-APO3G1 (5′-TATCCCTTGTACACTTTGT-3′) for cDNA synthesis, RACE-APO3G2 (5′-CATACTCCTGGTCACGAT-3′) for the first PCR and RACE-APO3Gnest (5′-GAATACACCTGGCCTCGAA-3′) for the nested PCR.
T72 493-670 Sentence denotes Reaction products were analyzed by agarose gel electrophoresis, purified using QIAquick gel extraction kit (Qiagen), T/A-cloned into vector pCR4-TOPO (Invitrogen) and sequenced.

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3870 0-8 JJ denotes 5′-Rapid
T3871 9-22 NN denotes amplification
T3872 23-25 IN denotes of
T3873 26-30 NN denotes cDNA
T3874 31-35 VB denotes ends
T3875 36-44 NN denotes analysis
T3876 45-46 -LRB- denotes (
T3877 46-50 NN denotes RACE
T3878 50-51 -RRB- denotes )
T3879 52-57 JJ denotes Total
T3880 58-61 NN denotes RNA
T3881 62-65 VB denotes was
T3882 66-74 VB denotes isolated
T3883 75-79 IN denotes from
T3884 80-87 NN denotes A3.01 T
T3885 88-93 NN denotes cells
T3886 94-99 VB denotes using
T3887 100-106 NN denotes RNeasy
T3888 107-111 NN denotes mini
T3889 112-115 NN denotes kit
T3890 116-117 -LRB- denotes (
T3891 117-123 NN denotes Qiagen
T3892 123-124 -RRB- denotes )
T3893 126-129 DT denotes The
T3894 130-145 JJ denotes transcriptional
T3895 146-151 NN denotes start
T3896 152-157 NN denotes sites
T3897 158-160 IN denotes of
T3898 161-164 NN denotes A3G
T3899 165-169 VB denotes were
T3900 170-180 VB denotes identified
T3901 181-186 VB denotes using
T3902 187-190 DT denotes the
T3903 191-196 NN denotes 5′/3′
T3904 197-201 NN denotes RACE
T3905 202-205 NN denotes Kit
T3906 205-206 -COMMA- denotes ,
T3907 207-210 NN denotes 2nd
T3908 211-221 NN denotes Generation
T3909 222-223 -LRB- denotes (
T3910 223-228 NNP denotes Roche
T3911 228-229 -RRB- denotes )
T3912 230-239 VB denotes according
T3913 240-242 TO denotes to
T3914 243-246 DT denotes the
T3915 247-259 NN denotes manufacturer
T3916 259-261 POS denotes 's
T3917 262-274 NN denotes instructions
T3918 276-279 DT denotes The
T3919 280-289 VB denotes following
T3920 290-297 NN denotes primers
T3921 298-302 VB denotes were
T3922 303-307 VB denotes used
T3923 307-308 -COLON- denotes :
T3924 309-320 NN denotes RACE-APO3G1
T3925 321-322 -LRB- denotes (
T3926 322-347 NN denotes 5′-TATCCCTTGTACACTTTGT-3′
T3927 347-348 -RRB- denotes )
T3928 349-352 IN denotes for
T3929 353-357 NN denotes cDNA
T3930 358-367 NN denotes synthesis
T3931 367-368 -COMMA- denotes ,
T3932 369-380 NN denotes RACE-APO3G2
T3933 381-382 -LRB- denotes (
T3934 382-406 NN denotes 5′-CATACTCCTGGTCACGAT-3′
T3935 406-407 -RRB- denotes )
T3936 408-411 IN denotes for
T3937 412-415 DT denotes the
T3938 416-421 JJ denotes first
T3939 422-425 NN denotes PCR
T3940 426-429 CC denotes and
T3941 430-444 NN denotes RACE-APO3Gnest
T3942 445-446 -LRB- denotes (
T3943 446-471 NN denotes 5′-GAATACACCTGGCCTCGAA-3′
T3944 471-472 -RRB- denotes )
T3945 473-476 IN denotes for
T3946 477-480 DT denotes the
T3947 481-487 JJ denotes nested
T3948 488-491 NN denotes PCR
T3949 493-501 NN denotes Reaction
T3950 502-510 NN denotes products
T3951 511-515 VB denotes were
T3952 516-524 VB denotes analyzed
T3953 525-527 IN denotes by
T3954 528-535 NN denotes agarose
T3955 536-539 NN denotes gel
T3956 540-555 NN denotes electrophoresis
T3957 555-556 -COMMA- denotes ,
T3958 557-565 VB denotes purified
T3959 566-571 VB denotes using
T3960 572-580 JJ denotes QIAquick
T3961 581-584 NN denotes gel
T3962 585-595 NN denotes extraction
T3963 596-599 NN denotes kit
T3964 600-601 -LRB- denotes (
T3965 601-607 NN denotes Qiagen
T3966 607-608 -RRB- denotes )
T3967 608-609 -COMMA- denotes ,
T3968 610-620 JJ denotes T/A-cloned
T3969 621-625 IN denotes into
T3970 626-632 NN denotes vector
T3971 633-642 NN denotes pCR4-TOPO
T3972 643-644 -LRB- denotes (
T3973 644-654 NN denotes Invitrogen
T3974 654-655 -RRB- denotes )
T3975 656-659 CC denotes and
T3976 660-669 VB denotes sequenced
R3145 T3871 T3870 arg1Of amplification,5′-Rapid
R3146 T3871 T3872 arg1Of amplification,of
R3147 T3871 T3874 arg1Of amplification,ends
R3148 T3873 T3872 arg2Of cDNA,of
R3149 T3875 T3874 arg2Of analysis,ends
R3150 T3875 T3876 arg1Of analysis,(
R3151 T3877 T3876 arg2Of RACE,(
R3152 T3878 T3876 arg3Of ),(
R3153 T3880 T3879 arg1Of RNA,Total
R3154 T3880 T3881 arg1Of RNA,was
R3155 T3880 T3882 arg2Of RNA,isolated
R3156 T3882 T3881 arg2Of isolated,was
R3157 T3882 T3883 arg1Of isolated,from
R3158 T3885 T3883 arg2Of cells,from
R3159 T3885 T3884 arg1Of cells,A3.01 T
R3160 T3886 T3882 arg3Of using,isolated
R3161 T3889 T3886 arg2Of kit,using
R3162 T3889 T3887 arg1Of kit,RNeasy
R3163 T3889 T3888 arg1Of kit,mini
R3164 T3889 T3890 arg1Of kit,(
R3165 T3891 T3890 arg2Of Qiagen,(
R3166 T3892 T3890 arg3Of ),(
R3167 T3896 T3893 arg1Of sites,The
R3168 T3896 T3894 arg1Of sites,transcriptional
R3169 T3896 T3895 arg1Of sites,start
R3170 T3896 T3897 arg1Of sites,of
R3171 T3896 T3899 arg1Of sites,were
R3172 T3896 T3900 arg2Of sites,identified
R3173 T3898 T3897 arg2Of A3G,of
R3174 T3900 T3899 arg2Of identified,were
R3175 T3901 T3900 arg3Of using,identified
R3176 T3901 T3909 arg1Of using,(
R3177 T3901 T3912 arg1Of using,according
R3178 T3905 T3901 arg2Of Kit,using
R3179 T3905 T3902 arg1Of Kit,the
R3180 T3905 T3903 arg1Of Kit,5′/3′
R3181 T3905 T3904 arg1Of Kit,RACE
R3182 T3905 T3906 arg1Of Kit,","
R3183 T3908 T3906 arg2Of Generation,","
R3184 T3908 T3907 arg1Of Generation,2nd
R3185 T3910 T3909 arg2Of Roche,(
R3186 T3911 T3909 arg3Of ),(
R3187 T3913 T3912 arg2Of to,according
R3188 T3915 T3914 arg1Of manufacturer,the
R3189 T3915 T3916 arg2Of manufacturer,'s
R3190 T3917 T3913 arg2Of instructions,to
R3191 T3917 T3916 arg1Of instructions,'s
R3192 T3920 T3918 arg1Of primers,The
R3193 T3920 T3919 arg1Of primers,following
R3194 T3920 T3921 arg1Of primers,were
R3195 T3920 T3922 arg2Of primers,used
R3196 T3922 T3921 arg2Of used,were
R3197 T3922 T3923 arg1Of used,:
R3198 T3922 T3924 arg1Of used,RACE-APO3G1
R3199 T3922 T3928 arg1Of used,for
R3200 T3922 T3945 arg1Of used,for
R3201 T3924 T3925 arg1Of RACE-APO3G1,(
R3202 T3926 T3925 arg2Of 5′-TATCCCTTGTACACTTTGT-3′,(
R3203 T3927 T3925 arg3Of ),(
R3204 T3930 T3929 arg1Of synthesis,cDNA
R3205 T3930 T3931 arg1Of synthesis,","
R3206 T3931 T3940 arg1Of ",",and
R3207 T3932 T3931 arg2Of RACE-APO3G2,","
R3208 T3932 T3933 arg1Of RACE-APO3G2,(
R3209 T3932 T3936 arg1Of RACE-APO3G2,for
R3210 T3934 T3933 arg2Of 5′-CATACTCCTGGTCACGAT-3′,(
R3211 T3935 T3933 arg3Of ),(
R3212 T3939 T3936 arg2Of PCR,for
R3213 T3939 T3937 arg1Of PCR,the
R3214 T3939 T3938 arg1Of PCR,first
R3215 T3940 T3928 arg2Of and,for
R3216 T3941 T3940 arg2Of RACE-APO3Gnest,and
R3217 T3941 T3942 arg1Of RACE-APO3Gnest,(
R3218 T3943 T3942 arg2Of 5′-GAATACACCTGGCCTCGAA-3′,(
R3219 T3944 T3942 arg3Of ),(
R3220 T3948 T3945 arg2Of PCR,for
R3221 T3948 T3946 arg1Of PCR,the
R3222 T3948 T3947 arg1Of PCR,nested
R3223 T3950 T3949 arg1Of products,Reaction
R3224 T3950 T3951 arg1Of products,were
R3225 T3950 T3952 arg2Of products,analyzed
R3235 T3956 T3952 arg1Of electrophoresis,analyzed
R3236 T3956 T3953 arg2Of electrophoresis,by
R3252 T3975 T3951 arg2Of and,were
R3226 T3950 T3958 arg2Of products,purified
R3227 T3950 T3968 arg1Of products,T/A-cloned
R3228 T3950 T3976 arg2Of products,sequenced
R3229 T3952 T3957 arg1Of analyzed,","
R3230 T3952 T3958 modOf analyzed,purified
R3231 T3952 T3959 modOf analyzed,using
R3232 T3952 T3967 arg1Of analyzed,","
R3233 T3952 T3968 modOf analyzed,T/A-cloned
R3234 T3952 T3975 arg1Of analyzed,and
R3237 T3956 T3954 arg1Of electrophoresis,agarose
R3238 T3956 T3955 arg1Of electrophoresis,gel
R3239 T3963 T3959 arg2Of kit,using
R3240 T3963 T3960 arg1Of kit,QIAquick
R3241 T3963 T3961 arg1Of kit,gel
R3242 T3963 T3962 arg1Of kit,extraction
R3243 T3963 T3964 arg1Of kit,(
R3244 T3965 T3964 arg2Of Qiagen,(
R3245 T3966 T3964 arg3Of ),(
R3246 T3968 T3969 arg1Of T/A-cloned,into
R3247 T3971 T3969 arg2Of pCR4-TOPO,into
R3248 T3971 T3970 arg1Of pCR4-TOPO,vector
R3249 T3971 T3972 arg1Of pCR4-TOPO,(
R3250 T3973 T3972 arg2Of Invitrogen,(
R3251 T3974 T3972 arg3Of ),(
R3253 T3976 T3975 arg2Of sequenced,and

events-check-again

Id Subject Object Predicate Lexical cue
T4121 161-164 Protein denotes A3G

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T4122 161-164 Protein denotes A3G
T4123 369-373 Protein denotes RACE
T4124 374-380 Protein denotes APO3G2

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T3862 161-164 Protein denotes A3G

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T3977 161-164 Q9HC16 denotes A3G