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PMC:1914394 / 59183-60138 JSONTXT

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Id Subject Object Predicate Lexical cue
T31605 66-68 NN denotes BD
T31606 69-70 CD denotes 9
T31592 0-5 NN denotes Mouse
T31593 6-13 NNS denotes retinas
T31594 19-30 VBN denotes homogenized
T31595 14-18 VBD denotes were
T31596 31-33 IN denotes by
T31597 34-41 VBG denotes passing
T31598 42-46 PRP denotes them
T31599 47-54 IN denotes through
T31600 55-56 DT denotes a
T31601 90-96 NN denotes needle
T31602 57-59 CD denotes 30
T31603 60-65 NN denotes gauge
T31604 59-60 HYPH denotes -
T31607 71-88 NN denotes http://www.bd.com
T31608 88-89 -RRB- denotes )
T31609 97-98 CD denotes 5
T31610 99-101 CD denotes 10
T31611 98-99 SYM denotes –
T31612 102-107 NNS denotes times
T31613 108-110 IN denotes in
T31614 111-112 CD denotes 1
T31615 118-126 NN denotes solution
T31616 112-113 SYM denotes ×
T31617 114-117 NN denotes PBS
T31618 126-127 . denotes .
T31619 127-297 sentence denotes Nuclear and cytoplasmic proteins were extracted using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Product# 78833, Pierce Biotechnology, http://www.piercenet.com).
T31620 128-135 JJ denotes Nuclear
T31621 152-160 NN denotes proteins
T31622 136-139 CC denotes and
T31623 140-151 JJ denotes cytoplasmic
T31624 166-175 VBN denotes extracted
T31625 161-165 VBD denotes were
T31626 176-181 VBG denotes using
T31627 182-185 DT denotes the
T31628 228-231 NN denotes Kit
T31629 186-188 NN denotes NE
T31630 189-192 NN denotes PER
T31631 188-189 HYPH denotes -
T31632 193-200 JJ denotes Nuclear
T31633 201-204 CC denotes and
T31634 205-216 JJ denotes Cytoplasmic
T31635 217-227 NN denotes Extraction
T31636 232-233 -LRB- denotes (
T31637 233-240 NN denotes Product
T31638 240-241 SYM denotes #
T31639 242-247 CD denotes 78833
T31640 247-249 , denotes ,
T31641 256-269 NNP denotes Biotechnology
T31642 249-255 NNP denotes Pierce
T31643 269-271 , denotes ,
T31644 271-295 NN denotes http://www.piercenet.com
T31645 295-296 -RRB- denotes )
T31646 296-297 . denotes .
T31647 297-372 sentence denotes Proteins were separated by 10% SDS-PAGE and transferred to nitrocellulose.
T31648 298-306 NNS denotes Proteins
T31649 312-321 VBN denotes separated
T31650 307-311 VBD denotes were
T31651 322-324 IN denotes by
T31652 325-327 CD denotes 10
T31653 327-328 NN denotes %
T31654 333-337 NN denotes PAGE
T31655 329-332 NN denotes SDS
T31656 332-333 HYPH denotes -
T31657 338-341 CC denotes and
T31658 342-353 VBN denotes transferred
T31659 354-356 IN denotes to
T31660 357-371 NN denotes nitrocellulose
T31661 371-372 . denotes .
T31662 372-500 sentence denotes After blocking overnight at 4 °C in 5% skim milk, membranes were incubated in the primary antibody for 2 h at room temperature.
T31663 373-378 IN denotes After
T31664 438-447 VBN denotes incubated
T31665 379-387 VBG denotes blocking
T31666 388-397 RB denotes overnight
T31667 398-400 IN denotes at
T31668 401-402 CD denotes 4
T31669 403-405 NN denotes °C
T31670 406-408 IN denotes in
T31671 409-410 CD denotes 5
T31672 410-411 NN denotes %
T31673 417-421 NN denotes milk
T31674 412-416 NN denotes skim
T31675 421-423 , denotes ,
T31676 423-432 NNS denotes membranes
T31677 433-437 VBD denotes were
T31678 448-450 IN denotes in
T31679 451-454 DT denotes the
T31680 463-471 NN denotes antibody
T31681 455-462 JJ denotes primary
T31682 472-475 IN denotes for
T31683 476-477 CD denotes 2
T31684 478-479 NN denotes h
T31685 480-482 IN denotes at
T31686 483-487 NN denotes room
T31687 488-499 NN denotes temperature
T31688 499-500 . denotes .
T31689 500-780 sentence denotes After three 10-min washes in TPBS (100 mM Na2HPO4, 100 mM NaH2PO4, 0.5 N NaCl, 0.1% Tween-20), membranes were incubated for 30 min at room temperature in the secondary horseradish peroxidase-conjugated antibody (Jackson ImmunoResearch Laboratories, http://www.jacksonimmuno.com).
T31690 501-506 IN denotes After
T31691 611-620 VBN denotes incubated
T31692 507-512 CD denotes three
T31693 520-526 NNS denotes washes
T31694 513-515 CD denotes 10
T31695 516-519 NN denotes min
T31696 515-516 HYPH denotes -
T31697 527-529 IN denotes in
T31698 530-534 NN denotes TPBS
T31699 535-536 -LRB- denotes (
T31700 536-539 CD denotes 100
T31701 540-542 NN denotes mM
T31702 543-550 NN denotes Na2HPO4
T31703 550-552 , denotes ,
T31704 552-555 CD denotes 100
T31705 556-558 NN denotes mM
T31706 559-566 NN denotes NaH2PO4
T31707 566-568 , denotes ,
T31708 568-571 CD denotes 0.5
T31709 572-573 NN denotes N
T31710 574-578 NN denotes NaCl
T31711 578-580 , denotes ,
T31712 580-583 CD denotes 0.1
T31713 583-584 NN denotes %
T31714 585-590 NN denotes Tween
T31715 590-591 HYPH denotes -
T31716 591-593 CD denotes 20
T31717 593-594 -RRB- denotes )
T31718 594-596 , denotes ,
T31719 596-605 NNS denotes membranes
T31720 606-610 VBD denotes were
T31721 621-624 IN denotes for
T31722 625-627 CD denotes 30
T31723 628-631 NN denotes min
T31724 632-634 IN denotes at
T31725 635-639 NN denotes room
T31726 640-651 NN denotes temperature
T31727 652-654 IN denotes in
T31728 655-658 DT denotes the
T31729 703-711 NN denotes antibody
T31730 659-668 JJ denotes secondary
T31731 669-680 NN denotes horseradish
T31732 681-691 NN denotes peroxidase
T31733 692-702 VBN denotes conjugated
T31734 691-692 HYPH denotes -
T31735 712-713 -LRB- denotes (
T31736 736-748 NNP denotes Laboratories
T31737 713-720 NNP denotes Jackson
T31738 721-735 NNP denotes ImmunoResearch
T31739 748-750 , denotes ,
T31740 750-778 NN denotes http://www.jacksonimmuno.com
T31741 778-779 -RRB- denotes )
T31742 779-780 . denotes .
T31743 780-955 sentence denotes Blots were developed using the ECL-Plus chemiluminescent detection system (Amersham Pharmacia Biotech, http://www.pharmacia.ca), according to the manufacturer's instructions.
T31744 781-786 NNS denotes Blots
T31745 792-801 VBN denotes developed
T31746 787-791 VBD denotes were
T31747 802-807 VBG denotes using
T31748 808-811 DT denotes the
T31749 848-854 NN denotes system
T31750 812-815 NN denotes ECL
T31751 816-820 NN denotes Plus
T31752 815-816 HYPH denotes -
T31753 821-837 JJ denotes chemiluminescent
T31754 838-847 NN denotes detection
T31755 855-856 -LRB- denotes (
T31756 875-882 NNP denotes Biotech
T31757 856-864 NNP denotes Amersham
T31758 865-874 NNP denotes Pharmacia
T31759 882-884 , denotes ,
T31760 884-907 NN denotes http://www.pharmacia.ca
T31761 907-908 -RRB- denotes )
T31762 908-910 , denotes ,
T31763 910-919 VBG denotes according
T31764 920-922 IN denotes to
T31765 923-926 DT denotes the
T31766 927-939 NN denotes manufacturer
T31767 942-954 NNS denotes instructions
T31768 939-941 POS denotes 's
T31769 954-955 . denotes .
R9428 T31592 T31593 compound Mouse,retinas
R9429 T31593 T31594 nsubjpass retinas,homogenized
R9430 T31595 T31594 auxpass were,homogenized
R9431 T31596 T31594 prep by,homogenized
R9432 T31597 T31596 pcomp passing,by
R9433 T31598 T31597 dobj them,passing
R9434 T31599 T31597 prep through,passing
R9435 T31600 T31601 det a,needle
R9436 T31601 T31599 pobj needle,through
R9437 T31602 T31603 nummod 30,gauge
R9438 T31603 T31601 nmod gauge,needle
R9439 T31604 T31603 punct -,gauge
R9440 T31605 T31601 nmod BD,needle
R9441 T31606 T31605 nummod 9,BD
R9442 T31607 T31605 appos http://www.bd.com,BD
R9443 T31608 T31601 punct ),needle
R9444 T31609 T31610 quantmod 5,10
R9445 T31610 T31612 nummod 10,times
R9446 T31611 T31610 punct –,10
R9447 T31612 T31594 npadvmod times,homogenized
R9448 T31613 T31594 prep in,homogenized
R9449 T31614 T31615 nummod 1,solution
R9450 T31615 T31613 pobj solution,in
R9451 T31616 T31614 punct ×,1
R9452 T31617 T31615 compound PBS,solution
R9453 T31618 T31594 punct .,homogenized
R9454 T31620 T31621 amod Nuclear,proteins
R9455 T31621 T31624 nsubjpass proteins,extracted
R9456 T31622 T31620 cc and,Nuclear
R9457 T31623 T31620 conj cytoplasmic,Nuclear
R9458 T31625 T31624 auxpass were,extracted
R9459 T31626 T31624 advcl using,extracted
R9460 T31627 T31628 det the,Kit
R9461 T31628 T31626 dobj Kit,using
R9462 T31629 T31630 nmod NE,PER
R9463 T31630 T31628 nmod PER,Kit
R9464 T31631 T31630 punct -,PER
R9465 T31632 T31628 amod Nuclear,Kit
R9466 T31633 T31632 cc and,Nuclear
R9467 T31634 T31632 conj Cytoplasmic,Nuclear
R9468 T31635 T31628 compound Extraction,Kit
R9469 T31636 T31628 punct (,Kit
R9470 T31637 T31628 appos Product,Kit
R9471 T31638 T31637 punct #,Product
R9472 T31639 T31637 nummod 78833,Product
R9473 T31640 T31641 punct ", ",Biotechnology
R9474 T31641 T31628 parataxis Biotechnology,Kit
R9475 T31642 T31641 compound Pierce,Biotechnology
R9476 T31643 T31641 punct ", ",Biotechnology
R9477 T31644 T31641 npadvmod http://www.piercenet.com,Biotechnology
R9478 T31645 T31641 punct ),Biotechnology
R9479 T31646 T31624 punct .,extracted
R9480 T31648 T31649 nsubjpass Proteins,separated
R9481 T31650 T31649 auxpass were,separated
R9482 T31651 T31649 prep by,separated
R9483 T31652 T31653 nummod 10,%
R9484 T31653 T31654 compound %,PAGE
R9485 T31654 T31651 pobj PAGE,by
R9486 T31655 T31654 compound SDS,PAGE
R9487 T31656 T31654 punct -,PAGE
R9488 T31657 T31649 cc and,separated
R9489 T31658 T31649 conj transferred,separated
R9490 T31659 T31658 prep to,transferred
R9491 T31660 T31659 pobj nitrocellulose,to
R9492 T31661 T31649 punct .,separated
R9493 T31663 T31664 prep After,incubated
R9494 T31665 T31663 pcomp blocking,After
R9495 T31666 T31665 advmod overnight,blocking
R9496 T31667 T31665 prep at,blocking
R9497 T31668 T31669 nummod 4,°C
R9498 T31669 T31667 pobj °C,at
R9499 T31670 T31665 prep in,blocking
R9500 T31671 T31672 nummod 5,%
R9501 T31672 T31673 compound %,milk
R9502 T31673 T31670 pobj milk,in
R9503 T31674 T31673 compound skim,milk
R9504 T31675 T31664 punct ", ",incubated
R9505 T31676 T31664 nsubjpass membranes,incubated
R9506 T31677 T31664 auxpass were,incubated
R9507 T31678 T31664 prep in,incubated
R9508 T31679 T31680 det the,antibody
R9509 T31680 T31678 pobj antibody,in
R9510 T31681 T31680 amod primary,antibody
R9511 T31682 T31664 prep for,incubated
R9512 T31683 T31684 nummod 2,h
R9513 T31684 T31682 pobj h,for
R9514 T31685 T31664 prep at,incubated
R9515 T31686 T31687 compound room,temperature
R9516 T31687 T31685 pobj temperature,at
R9517 T31688 T31664 punct .,incubated
R9518 T31690 T31691 prep After,incubated
R9519 T31692 T31693 nummod three,washes
R9520 T31693 T31690 pobj washes,After
R9521 T31694 T31695 nummod 10,min
R9522 T31695 T31693 compound min,washes
R9523 T31696 T31695 punct -,min
R9524 T31697 T31693 prep in,washes
R9525 T31698 T31697 pobj TPBS,in
R9526 T31699 T31698 punct (,TPBS
R9527 T31700 T31701 nummod 100,mM
R9528 T31701 T31702 compound mM,Na2HPO4
R9529 T31702 T31698 appos Na2HPO4,TPBS
R9530 T31703 T31702 punct ", ",Na2HPO4
R9531 T31704 T31705 nummod 100,mM
R9532 T31705 T31706 compound mM,NaH2PO4
R9533 T31706 T31702 appos NaH2PO4,Na2HPO4
R9534 T31707 T31702 punct ", ",Na2HPO4
R9535 T31708 T31709 nummod 0.5,N
R9536 T31709 T31710 compound N,NaCl
R9537 T31710 T31702 appos NaCl,Na2HPO4
R9538 T31711 T31702 punct ", ",Na2HPO4
R9539 T31712 T31713 nummod 0.1,%
R9540 T31713 T31714 compound %,Tween
R9541 T31714 T31702 appos Tween,Na2HPO4
R9542 T31715 T31714 punct -,Tween
R9543 T31716 T31714 nummod 20,Tween
R9544 T31717 T31691 punct ),incubated
R9545 T31718 T31691 punct ", ",incubated
R9546 T31719 T31691 nsubjpass membranes,incubated
R9547 T31720 T31691 auxpass were,incubated
R9548 T31721 T31691 prep for,incubated
R9549 T31722 T31723 nummod 30,min
R9550 T31723 T31721 pobj min,for
R9551 T31724 T31691 prep at,incubated
R9552 T31725 T31726 compound room,temperature
R9553 T31726 T31724 pobj temperature,at
R9554 T31727 T31691 prep in,incubated
R9555 T31728 T31729 det the,antibody
R9556 T31729 T31727 pobj antibody,in
R9557 T31730 T31729 amod secondary,antibody
R9558 T31731 T31732 compound horseradish,peroxidase
R9559 T31732 T31733 npadvmod peroxidase,conjugated
R9560 T31733 T31729 amod conjugated,antibody
R9561 T31734 T31733 punct -,conjugated
R9562 T31735 T31736 punct (,Laboratories
R9563 T31736 T31729 parataxis Laboratories,antibody
R9564 T31737 T31736 compound Jackson,Laboratories
R9565 T31738 T31736 compound ImmunoResearch,Laboratories
R9566 T31739 T31736 punct ", ",Laboratories
R9567 T31740 T31736 npadvmod http://www.jacksonimmuno.com,Laboratories
R9568 T31741 T31736 punct ),Laboratories
R9569 T31742 T31691 punct .,incubated
R9570 T31744 T31745 nsubjpass Blots,developed
R9571 T31746 T31745 auxpass were,developed
R9572 T31747 T31745 advcl using,developed
R9573 T31748 T31749 det the,system
R9574 T31749 T31747 dobj system,using
R9575 T31750 T31751 nmod ECL,Plus
R9576 T31751 T31749 nmod Plus,system
R9577 T31752 T31751 punct -,Plus
R9578 T31753 T31749 amod chemiluminescent,system
R9579 T31754 T31749 compound detection,system
R9580 T31755 T31756 punct (,Biotech
R9581 T31756 T31749 parataxis Biotech,system
R9582 T31757 T31756 compound Amersham,Biotech
R9583 T31758 T31756 compound Pharmacia,Biotech
R9584 T31759 T31756 punct ", ",Biotech
R9585 T31760 T31756 npadvmod http://www.pharmacia.ca,Biotech
R9586 T31761 T31756 punct ),Biotech
R9587 T31762 T31747 punct ", ",using
R9588 T31763 T31747 prep according,using
R9589 T31764 T31763 prep to,according
R9590 T31765 T31766 det the,manufacturer
R9591 T31766 T31767 poss manufacturer,instructions
R9592 T31767 T31764 pobj instructions,to
R9593 T31768 T31766 case 's,manufacturer
R9594 T31769 T31745 punct .,developed

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T31552 0-5 NCBITaxon:10088 denotes Mouse
T31553 6-13 UBERON:0000966 denotes retinas
T31554 34-41 GO_EXT:biological_movement_or_translocation_process denotes passing
T31555 118-126 CHEBI:75958 denotes solution
T31556 128-135 GO:0005634 denotes Nuclear
T31557 140-151 GO:0005737 denotes cytoplasmic
T31558 152-160 CHEBI_PR_EXT:protein denotes proteins
T31559 193-200 GO:0005634 denotes Nuclear
T31560 205-216 GO:0005737 denotes Cytoplasmic
T31561 298-306 CHEBI_PR_EXT:protein denotes Proteins
T31562 329-332 CHEBI:8984 denotes SDS
T31563 342-353 GO_EXT:biological_movement_or_translocation_process denotes transferred
T31564 357-371 CHEBI:53325 denotes nitrocellulose
T31565 417-421 UBERON:0001913 denotes milk
T31566 463-471 GO:0042571 denotes antibody
T31567 543-550 CHEBI:34683 denotes Na2HPO4
T31568 559-566 CHEBI:37585 denotes NaH2PO4
T31569 574-578 CHEBI:26710 denotes NaCl
T31570 585-593 CHEBI:53424 denotes Tween-20
T31571 669-680 NCBITaxon:3704 denotes horseradish
T31572 681-691 GO_EXT:0004601 denotes peroxidase
T31573 692-702 MOP:0000779 denotes conjugated
T31574 703-711 GO:0042571 denotes antibody

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T31521 0-5 NCBITaxon:10088 denotes Mouse
T31522 6-13 UBERON:0000966 denotes retinas
T31523 118-126 CHEBI:75958 denotes solution
T31524 128-135 GO:0005634 denotes Nuclear
T31525 140-151 GO:0005737 denotes cytoplasmic
T31526 193-200 GO:0005634 denotes Nuclear
T31527 205-216 GO:0005737 denotes Cytoplasmic
T31528 329-332 CHEBI:8984 denotes SDS
T31529 357-371 CHEBI:53325 denotes nitrocellulose
T31530 417-421 UBERON:0001913 denotes milk
T31531 463-471 GO:0042571 denotes antibody
T31532 543-550 CHEBI:34683 denotes Na2HPO4
T31533 559-566 CHEBI:37585 denotes NaH2PO4
T31534 574-578 CHEBI:26710 denotes NaCl
T31535 585-593 CHEBI:53424 denotes Tween-20
T31536 669-680 NCBITaxon:3704 denotes horseradish
T31537 692-702 MOP:0000779 denotes conjugated
T31538 703-711 GO:0042571 denotes antibody