Id |
Subject |
Object |
Predicate |
Lexical cue |
T31605 |
66-68 |
NN |
denotes |
BD |
T31606 |
69-70 |
CD |
denotes |
9 |
T31592 |
0-5 |
NN |
denotes |
Mouse |
T31593 |
6-13 |
NNS |
denotes |
retinas |
T31594 |
19-30 |
VBN |
denotes |
homogenized |
T31595 |
14-18 |
VBD |
denotes |
were |
T31596 |
31-33 |
IN |
denotes |
by |
T31597 |
34-41 |
VBG |
denotes |
passing |
T31598 |
42-46 |
PRP |
denotes |
them |
T31599 |
47-54 |
IN |
denotes |
through |
T31600 |
55-56 |
DT |
denotes |
a |
T31601 |
90-96 |
NN |
denotes |
needle |
T31602 |
57-59 |
CD |
denotes |
30 |
T31603 |
60-65 |
NN |
denotes |
gauge |
T31604 |
59-60 |
HYPH |
denotes |
- |
T31607 |
71-88 |
NN |
denotes |
http://www.bd.com |
T31608 |
88-89 |
-RRB- |
denotes |
) |
T31609 |
97-98 |
CD |
denotes |
5 |
T31610 |
99-101 |
CD |
denotes |
10 |
T31611 |
98-99 |
SYM |
denotes |
– |
T31612 |
102-107 |
NNS |
denotes |
times |
T31613 |
108-110 |
IN |
denotes |
in |
T31614 |
111-112 |
CD |
denotes |
1 |
T31615 |
118-126 |
NN |
denotes |
solution |
T31616 |
112-113 |
SYM |
denotes |
× |
T31617 |
114-117 |
NN |
denotes |
PBS |
T31618 |
126-127 |
. |
denotes |
. |
T31619 |
127-297 |
sentence |
denotes |
Nuclear and cytoplasmic proteins were extracted using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Product# 78833, Pierce Biotechnology, http://www.piercenet.com). |
T31620 |
128-135 |
JJ |
denotes |
Nuclear |
T31621 |
152-160 |
NN |
denotes |
proteins |
T31622 |
136-139 |
CC |
denotes |
and |
T31623 |
140-151 |
JJ |
denotes |
cytoplasmic |
T31624 |
166-175 |
VBN |
denotes |
extracted |
T31625 |
161-165 |
VBD |
denotes |
were |
T31626 |
176-181 |
VBG |
denotes |
using |
T31627 |
182-185 |
DT |
denotes |
the |
T31628 |
228-231 |
NN |
denotes |
Kit |
T31629 |
186-188 |
NN |
denotes |
NE |
T31630 |
189-192 |
NN |
denotes |
PER |
T31631 |
188-189 |
HYPH |
denotes |
- |
T31632 |
193-200 |
JJ |
denotes |
Nuclear |
T31633 |
201-204 |
CC |
denotes |
and |
T31634 |
205-216 |
JJ |
denotes |
Cytoplasmic |
T31635 |
217-227 |
NN |
denotes |
Extraction |
T31636 |
232-233 |
-LRB- |
denotes |
( |
T31637 |
233-240 |
NN |
denotes |
Product |
T31638 |
240-241 |
SYM |
denotes |
# |
T31639 |
242-247 |
CD |
denotes |
78833 |
T31640 |
247-249 |
, |
denotes |
, |
T31641 |
256-269 |
NNP |
denotes |
Biotechnology |
T31642 |
249-255 |
NNP |
denotes |
Pierce |
T31643 |
269-271 |
, |
denotes |
, |
T31644 |
271-295 |
NN |
denotes |
http://www.piercenet.com |
T31645 |
295-296 |
-RRB- |
denotes |
) |
T31646 |
296-297 |
. |
denotes |
. |
T31647 |
297-372 |
sentence |
denotes |
Proteins were separated by 10% SDS-PAGE and transferred to nitrocellulose. |
T31648 |
298-306 |
NNS |
denotes |
Proteins |
T31649 |
312-321 |
VBN |
denotes |
separated |
T31650 |
307-311 |
VBD |
denotes |
were |
T31651 |
322-324 |
IN |
denotes |
by |
T31652 |
325-327 |
CD |
denotes |
10 |
T31653 |
327-328 |
NN |
denotes |
% |
T31654 |
333-337 |
NN |
denotes |
PAGE |
T31655 |
329-332 |
NN |
denotes |
SDS |
T31656 |
332-333 |
HYPH |
denotes |
- |
T31657 |
338-341 |
CC |
denotes |
and |
T31658 |
342-353 |
VBN |
denotes |
transferred |
T31659 |
354-356 |
IN |
denotes |
to |
T31660 |
357-371 |
NN |
denotes |
nitrocellulose |
T31661 |
371-372 |
. |
denotes |
. |
T31662 |
372-500 |
sentence |
denotes |
After blocking overnight at 4 °C in 5% skim milk, membranes were incubated in the primary antibody for 2 h at room temperature. |
T31663 |
373-378 |
IN |
denotes |
After |
T31664 |
438-447 |
VBN |
denotes |
incubated |
T31665 |
379-387 |
VBG |
denotes |
blocking |
T31666 |
388-397 |
RB |
denotes |
overnight |
T31667 |
398-400 |
IN |
denotes |
at |
T31668 |
401-402 |
CD |
denotes |
4 |
T31669 |
403-405 |
NN |
denotes |
°C |
T31670 |
406-408 |
IN |
denotes |
in |
T31671 |
409-410 |
CD |
denotes |
5 |
T31672 |
410-411 |
NN |
denotes |
% |
T31673 |
417-421 |
NN |
denotes |
milk |
T31674 |
412-416 |
NN |
denotes |
skim |
T31675 |
421-423 |
, |
denotes |
, |
T31676 |
423-432 |
NNS |
denotes |
membranes |
T31677 |
433-437 |
VBD |
denotes |
were |
T31678 |
448-450 |
IN |
denotes |
in |
T31679 |
451-454 |
DT |
denotes |
the |
T31680 |
463-471 |
NN |
denotes |
antibody |
T31681 |
455-462 |
JJ |
denotes |
primary |
T31682 |
472-475 |
IN |
denotes |
for |
T31683 |
476-477 |
CD |
denotes |
2 |
T31684 |
478-479 |
NN |
denotes |
h |
T31685 |
480-482 |
IN |
denotes |
at |
T31686 |
483-487 |
NN |
denotes |
room |
T31687 |
488-499 |
NN |
denotes |
temperature |
T31688 |
499-500 |
. |
denotes |
. |
T31689 |
500-780 |
sentence |
denotes |
After three 10-min washes in TPBS (100 mM Na2HPO4, 100 mM NaH2PO4, 0.5 N NaCl, 0.1% Tween-20), membranes were incubated for 30 min at room temperature in the secondary horseradish peroxidase-conjugated antibody (Jackson ImmunoResearch Laboratories, http://www.jacksonimmuno.com). |
T31690 |
501-506 |
IN |
denotes |
After |
T31691 |
611-620 |
VBN |
denotes |
incubated |
T31692 |
507-512 |
CD |
denotes |
three |
T31693 |
520-526 |
NNS |
denotes |
washes |
T31694 |
513-515 |
CD |
denotes |
10 |
T31695 |
516-519 |
NN |
denotes |
min |
T31696 |
515-516 |
HYPH |
denotes |
- |
T31697 |
527-529 |
IN |
denotes |
in |
T31698 |
530-534 |
NN |
denotes |
TPBS |
T31699 |
535-536 |
-LRB- |
denotes |
( |
T31700 |
536-539 |
CD |
denotes |
100 |
T31701 |
540-542 |
NN |
denotes |
mM |
T31702 |
543-550 |
NN |
denotes |
Na2HPO4 |
T31703 |
550-552 |
, |
denotes |
, |
T31704 |
552-555 |
CD |
denotes |
100 |
T31705 |
556-558 |
NN |
denotes |
mM |
T31706 |
559-566 |
NN |
denotes |
NaH2PO4 |
T31707 |
566-568 |
, |
denotes |
, |
T31708 |
568-571 |
CD |
denotes |
0.5 |
T31709 |
572-573 |
NN |
denotes |
N |
T31710 |
574-578 |
NN |
denotes |
NaCl |
T31711 |
578-580 |
, |
denotes |
, |
T31712 |
580-583 |
CD |
denotes |
0.1 |
T31713 |
583-584 |
NN |
denotes |
% |
T31714 |
585-590 |
NN |
denotes |
Tween |
T31715 |
590-591 |
HYPH |
denotes |
- |
T31716 |
591-593 |
CD |
denotes |
20 |
T31717 |
593-594 |
-RRB- |
denotes |
) |
T31718 |
594-596 |
, |
denotes |
, |
T31719 |
596-605 |
NNS |
denotes |
membranes |
T31720 |
606-610 |
VBD |
denotes |
were |
T31721 |
621-624 |
IN |
denotes |
for |
T31722 |
625-627 |
CD |
denotes |
30 |
T31723 |
628-631 |
NN |
denotes |
min |
T31724 |
632-634 |
IN |
denotes |
at |
T31725 |
635-639 |
NN |
denotes |
room |
T31726 |
640-651 |
NN |
denotes |
temperature |
T31727 |
652-654 |
IN |
denotes |
in |
T31728 |
655-658 |
DT |
denotes |
the |
T31729 |
703-711 |
NN |
denotes |
antibody |
T31730 |
659-668 |
JJ |
denotes |
secondary |
T31731 |
669-680 |
NN |
denotes |
horseradish |
T31732 |
681-691 |
NN |
denotes |
peroxidase |
T31733 |
692-702 |
VBN |
denotes |
conjugated |
T31734 |
691-692 |
HYPH |
denotes |
- |
T31735 |
712-713 |
-LRB- |
denotes |
( |
T31736 |
736-748 |
NNP |
denotes |
Laboratories |
T31737 |
713-720 |
NNP |
denotes |
Jackson |
T31738 |
721-735 |
NNP |
denotes |
ImmunoResearch |
T31739 |
748-750 |
, |
denotes |
, |
T31740 |
750-778 |
NN |
denotes |
http://www.jacksonimmuno.com |
T31741 |
778-779 |
-RRB- |
denotes |
) |
T31742 |
779-780 |
. |
denotes |
. |
T31743 |
780-955 |
sentence |
denotes |
Blots were developed using the ECL-Plus chemiluminescent detection system (Amersham Pharmacia Biotech, http://www.pharmacia.ca), according to the manufacturer's instructions. |
T31744 |
781-786 |
NNS |
denotes |
Blots |
T31745 |
792-801 |
VBN |
denotes |
developed |
T31746 |
787-791 |
VBD |
denotes |
were |
T31747 |
802-807 |
VBG |
denotes |
using |
T31748 |
808-811 |
DT |
denotes |
the |
T31749 |
848-854 |
NN |
denotes |
system |
T31750 |
812-815 |
NN |
denotes |
ECL |
T31751 |
816-820 |
NN |
denotes |
Plus |
T31752 |
815-816 |
HYPH |
denotes |
- |
T31753 |
821-837 |
JJ |
denotes |
chemiluminescent |
T31754 |
838-847 |
NN |
denotes |
detection |
T31755 |
855-856 |
-LRB- |
denotes |
( |
T31756 |
875-882 |
NNP |
denotes |
Biotech |
T31757 |
856-864 |
NNP |
denotes |
Amersham |
T31758 |
865-874 |
NNP |
denotes |
Pharmacia |
T31759 |
882-884 |
, |
denotes |
, |
T31760 |
884-907 |
NN |
denotes |
http://www.pharmacia.ca |
T31761 |
907-908 |
-RRB- |
denotes |
) |
T31762 |
908-910 |
, |
denotes |
, |
T31763 |
910-919 |
VBG |
denotes |
according |
T31764 |
920-922 |
IN |
denotes |
to |
T31765 |
923-926 |
DT |
denotes |
the |
T31766 |
927-939 |
NN |
denotes |
manufacturer |
T31767 |
942-954 |
NNS |
denotes |
instructions |
T31768 |
939-941 |
POS |
denotes |
's |
T31769 |
954-955 |
. |
denotes |
. |
R9428 |
T31592 |
T31593 |
compound |
Mouse,retinas |
R9429 |
T31593 |
T31594 |
nsubjpass |
retinas,homogenized |
R9430 |
T31595 |
T31594 |
auxpass |
were,homogenized |
R9431 |
T31596 |
T31594 |
prep |
by,homogenized |
R9432 |
T31597 |
T31596 |
pcomp |
passing,by |
R9433 |
T31598 |
T31597 |
dobj |
them,passing |
R9434 |
T31599 |
T31597 |
prep |
through,passing |
R9435 |
T31600 |
T31601 |
det |
a,needle |
R9436 |
T31601 |
T31599 |
pobj |
needle,through |
R9437 |
T31602 |
T31603 |
nummod |
30,gauge |
R9438 |
T31603 |
T31601 |
nmod |
gauge,needle |
R9439 |
T31604 |
T31603 |
punct |
-,gauge |
R9440 |
T31605 |
T31601 |
nmod |
BD,needle |
R9441 |
T31606 |
T31605 |
nummod |
9,BD |
R9442 |
T31607 |
T31605 |
appos |
http://www.bd.com,BD |
R9443 |
T31608 |
T31601 |
punct |
),needle |
R9444 |
T31609 |
T31610 |
quantmod |
5,10 |
R9445 |
T31610 |
T31612 |
nummod |
10,times |
R9446 |
T31611 |
T31610 |
punct |
–,10 |
R9447 |
T31612 |
T31594 |
npadvmod |
times,homogenized |
R9448 |
T31613 |
T31594 |
prep |
in,homogenized |
R9449 |
T31614 |
T31615 |
nummod |
1,solution |
R9450 |
T31615 |
T31613 |
pobj |
solution,in |
R9451 |
T31616 |
T31614 |
punct |
×,1 |
R9452 |
T31617 |
T31615 |
compound |
PBS,solution |
R9453 |
T31618 |
T31594 |
punct |
.,homogenized |
R9454 |
T31620 |
T31621 |
amod |
Nuclear,proteins |
R9455 |
T31621 |
T31624 |
nsubjpass |
proteins,extracted |
R9456 |
T31622 |
T31620 |
cc |
and,Nuclear |
R9457 |
T31623 |
T31620 |
conj |
cytoplasmic,Nuclear |
R9458 |
T31625 |
T31624 |
auxpass |
were,extracted |
R9459 |
T31626 |
T31624 |
advcl |
using,extracted |
R9460 |
T31627 |
T31628 |
det |
the,Kit |
R9461 |
T31628 |
T31626 |
dobj |
Kit,using |
R9462 |
T31629 |
T31630 |
nmod |
NE,PER |
R9463 |
T31630 |
T31628 |
nmod |
PER,Kit |
R9464 |
T31631 |
T31630 |
punct |
-,PER |
R9465 |
T31632 |
T31628 |
amod |
Nuclear,Kit |
R9466 |
T31633 |
T31632 |
cc |
and,Nuclear |
R9467 |
T31634 |
T31632 |
conj |
Cytoplasmic,Nuclear |
R9468 |
T31635 |
T31628 |
compound |
Extraction,Kit |
R9469 |
T31636 |
T31628 |
punct |
(,Kit |
R9470 |
T31637 |
T31628 |
appos |
Product,Kit |
R9471 |
T31638 |
T31637 |
punct |
#,Product |
R9472 |
T31639 |
T31637 |
nummod |
78833,Product |
R9473 |
T31640 |
T31641 |
punct |
", ",Biotechnology |
R9474 |
T31641 |
T31628 |
parataxis |
Biotechnology,Kit |
R9475 |
T31642 |
T31641 |
compound |
Pierce,Biotechnology |
R9476 |
T31643 |
T31641 |
punct |
", ",Biotechnology |
R9477 |
T31644 |
T31641 |
npadvmod |
http://www.piercenet.com,Biotechnology |
R9478 |
T31645 |
T31641 |
punct |
),Biotechnology |
R9479 |
T31646 |
T31624 |
punct |
.,extracted |
R9480 |
T31648 |
T31649 |
nsubjpass |
Proteins,separated |
R9481 |
T31650 |
T31649 |
auxpass |
were,separated |
R9482 |
T31651 |
T31649 |
prep |
by,separated |
R9483 |
T31652 |
T31653 |
nummod |
10,% |
R9484 |
T31653 |
T31654 |
compound |
%,PAGE |
R9485 |
T31654 |
T31651 |
pobj |
PAGE,by |
R9486 |
T31655 |
T31654 |
compound |
SDS,PAGE |
R9487 |
T31656 |
T31654 |
punct |
-,PAGE |
R9488 |
T31657 |
T31649 |
cc |
and,separated |
R9489 |
T31658 |
T31649 |
conj |
transferred,separated |
R9490 |
T31659 |
T31658 |
prep |
to,transferred |
R9491 |
T31660 |
T31659 |
pobj |
nitrocellulose,to |
R9492 |
T31661 |
T31649 |
punct |
.,separated |
R9493 |
T31663 |
T31664 |
prep |
After,incubated |
R9494 |
T31665 |
T31663 |
pcomp |
blocking,After |
R9495 |
T31666 |
T31665 |
advmod |
overnight,blocking |
R9496 |
T31667 |
T31665 |
prep |
at,blocking |
R9497 |
T31668 |
T31669 |
nummod |
4,°C |
R9498 |
T31669 |
T31667 |
pobj |
°C,at |
R9499 |
T31670 |
T31665 |
prep |
in,blocking |
R9500 |
T31671 |
T31672 |
nummod |
5,% |
R9501 |
T31672 |
T31673 |
compound |
%,milk |
R9502 |
T31673 |
T31670 |
pobj |
milk,in |
R9503 |
T31674 |
T31673 |
compound |
skim,milk |
R9504 |
T31675 |
T31664 |
punct |
", ",incubated |
R9505 |
T31676 |
T31664 |
nsubjpass |
membranes,incubated |
R9506 |
T31677 |
T31664 |
auxpass |
were,incubated |
R9507 |
T31678 |
T31664 |
prep |
in,incubated |
R9508 |
T31679 |
T31680 |
det |
the,antibody |
R9509 |
T31680 |
T31678 |
pobj |
antibody,in |
R9510 |
T31681 |
T31680 |
amod |
primary,antibody |
R9511 |
T31682 |
T31664 |
prep |
for,incubated |
R9512 |
T31683 |
T31684 |
nummod |
2,h |
R9513 |
T31684 |
T31682 |
pobj |
h,for |
R9514 |
T31685 |
T31664 |
prep |
at,incubated |
R9515 |
T31686 |
T31687 |
compound |
room,temperature |
R9516 |
T31687 |
T31685 |
pobj |
temperature,at |
R9517 |
T31688 |
T31664 |
punct |
.,incubated |
R9518 |
T31690 |
T31691 |
prep |
After,incubated |
R9519 |
T31692 |
T31693 |
nummod |
three,washes |
R9520 |
T31693 |
T31690 |
pobj |
washes,After |
R9521 |
T31694 |
T31695 |
nummod |
10,min |
R9522 |
T31695 |
T31693 |
compound |
min,washes |
R9523 |
T31696 |
T31695 |
punct |
-,min |
R9524 |
T31697 |
T31693 |
prep |
in,washes |
R9525 |
T31698 |
T31697 |
pobj |
TPBS,in |
R9526 |
T31699 |
T31698 |
punct |
(,TPBS |
R9527 |
T31700 |
T31701 |
nummod |
100,mM |
R9528 |
T31701 |
T31702 |
compound |
mM,Na2HPO4 |
R9529 |
T31702 |
T31698 |
appos |
Na2HPO4,TPBS |
R9530 |
T31703 |
T31702 |
punct |
", ",Na2HPO4 |
R9531 |
T31704 |
T31705 |
nummod |
100,mM |
R9532 |
T31705 |
T31706 |
compound |
mM,NaH2PO4 |
R9533 |
T31706 |
T31702 |
appos |
NaH2PO4,Na2HPO4 |
R9534 |
T31707 |
T31702 |
punct |
", ",Na2HPO4 |
R9535 |
T31708 |
T31709 |
nummod |
0.5,N |
R9536 |
T31709 |
T31710 |
compound |
N,NaCl |
R9537 |
T31710 |
T31702 |
appos |
NaCl,Na2HPO4 |
R9538 |
T31711 |
T31702 |
punct |
", ",Na2HPO4 |
R9539 |
T31712 |
T31713 |
nummod |
0.1,% |
R9540 |
T31713 |
T31714 |
compound |
%,Tween |
R9541 |
T31714 |
T31702 |
appos |
Tween,Na2HPO4 |
R9542 |
T31715 |
T31714 |
punct |
-,Tween |
R9543 |
T31716 |
T31714 |
nummod |
20,Tween |
R9544 |
T31717 |
T31691 |
punct |
),incubated |
R9545 |
T31718 |
T31691 |
punct |
", ",incubated |
R9546 |
T31719 |
T31691 |
nsubjpass |
membranes,incubated |
R9547 |
T31720 |
T31691 |
auxpass |
were,incubated |
R9548 |
T31721 |
T31691 |
prep |
for,incubated |
R9549 |
T31722 |
T31723 |
nummod |
30,min |
R9550 |
T31723 |
T31721 |
pobj |
min,for |
R9551 |
T31724 |
T31691 |
prep |
at,incubated |
R9552 |
T31725 |
T31726 |
compound |
room,temperature |
R9553 |
T31726 |
T31724 |
pobj |
temperature,at |
R9554 |
T31727 |
T31691 |
prep |
in,incubated |
R9555 |
T31728 |
T31729 |
det |
the,antibody |
R9556 |
T31729 |
T31727 |
pobj |
antibody,in |
R9557 |
T31730 |
T31729 |
amod |
secondary,antibody |
R9558 |
T31731 |
T31732 |
compound |
horseradish,peroxidase |
R9559 |
T31732 |
T31733 |
npadvmod |
peroxidase,conjugated |
R9560 |
T31733 |
T31729 |
amod |
conjugated,antibody |
R9561 |
T31734 |
T31733 |
punct |
-,conjugated |
R9562 |
T31735 |
T31736 |
punct |
(,Laboratories |
R9563 |
T31736 |
T31729 |
parataxis |
Laboratories,antibody |
R9564 |
T31737 |
T31736 |
compound |
Jackson,Laboratories |
R9565 |
T31738 |
T31736 |
compound |
ImmunoResearch,Laboratories |
R9566 |
T31739 |
T31736 |
punct |
", ",Laboratories |
R9567 |
T31740 |
T31736 |
npadvmod |
http://www.jacksonimmuno.com,Laboratories |
R9568 |
T31741 |
T31736 |
punct |
),Laboratories |
R9569 |
T31742 |
T31691 |
punct |
.,incubated |
R9570 |
T31744 |
T31745 |
nsubjpass |
Blots,developed |
R9571 |
T31746 |
T31745 |
auxpass |
were,developed |
R9572 |
T31747 |
T31745 |
advcl |
using,developed |
R9573 |
T31748 |
T31749 |
det |
the,system |
R9574 |
T31749 |
T31747 |
dobj |
system,using |
R9575 |
T31750 |
T31751 |
nmod |
ECL,Plus |
R9576 |
T31751 |
T31749 |
nmod |
Plus,system |
R9577 |
T31752 |
T31751 |
punct |
-,Plus |
R9578 |
T31753 |
T31749 |
amod |
chemiluminescent,system |
R9579 |
T31754 |
T31749 |
compound |
detection,system |
R9580 |
T31755 |
T31756 |
punct |
(,Biotech |
R9581 |
T31756 |
T31749 |
parataxis |
Biotech,system |
R9582 |
T31757 |
T31756 |
compound |
Amersham,Biotech |
R9583 |
T31758 |
T31756 |
compound |
Pharmacia,Biotech |
R9584 |
T31759 |
T31756 |
punct |
", ",Biotech |
R9585 |
T31760 |
T31756 |
npadvmod |
http://www.pharmacia.ca,Biotech |
R9586 |
T31761 |
T31756 |
punct |
),Biotech |
R9587 |
T31762 |
T31747 |
punct |
", ",using |
R9588 |
T31763 |
T31747 |
prep |
according,using |
R9589 |
T31764 |
T31763 |
prep |
to,according |
R9590 |
T31765 |
T31766 |
det |
the,manufacturer |
R9591 |
T31766 |
T31767 |
poss |
manufacturer,instructions |
R9592 |
T31767 |
T31764 |
pobj |
instructions,to |
R9593 |
T31768 |
T31766 |
case |
's,manufacturer |
R9594 |
T31769 |
T31745 |
punct |
.,developed |