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PMC:1914394 / 59168-60498 JSONTXT

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Id Subject Object Predicate Lexical cue
T31605 81-83 NN denotes BD
T31606 84-85 CD denotes 9
T31588 0-7 NNP denotes Western
T31589 8-13 NNS denotes blots
T31590 13-14 . denotes .
T31591 14-142 sentence denotes Mouse retinas were homogenized by passing them through a 30-gauge BD 9 http://www.bd.com) needle 5–10 times in 1× PBS solution.
T31592 15-20 NN denotes Mouse
T31593 21-28 NNS denotes retinas
T31594 34-45 VBN denotes homogenized
T31595 29-33 VBD denotes were
T31596 46-48 IN denotes by
T31597 49-56 VBG denotes passing
T31598 57-61 PRP denotes them
T31599 62-69 IN denotes through
T31600 70-71 DT denotes a
T31601 105-111 NN denotes needle
T31602 72-74 CD denotes 30
T31603 75-80 NN denotes gauge
T31604 74-75 HYPH denotes -
T31607 86-103 NN denotes http://www.bd.com
T31608 103-104 -RRB- denotes )
T31609 112-113 CD denotes 5
T31610 114-116 CD denotes 10
T31611 113-114 SYM denotes
T31612 117-122 NNS denotes times
T31613 123-125 IN denotes in
T31614 126-127 CD denotes 1
T31615 133-141 NN denotes solution
T31616 127-128 SYM denotes ×
T31617 129-132 NN denotes PBS
T31618 141-142 . denotes .
T31619 142-312 sentence denotes Nuclear and cytoplasmic proteins were extracted using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Product# 78833, Pierce Biotechnology, http://www.piercenet.com).
T31620 143-150 JJ denotes Nuclear
T31621 167-175 NN denotes proteins
T31622 151-154 CC denotes and
T31623 155-166 JJ denotes cytoplasmic
T31624 181-190 VBN denotes extracted
T31625 176-180 VBD denotes were
T31626 191-196 VBG denotes using
T31627 197-200 DT denotes the
T31628 243-246 NN denotes Kit
T31629 201-203 NN denotes NE
T31630 204-207 NN denotes PER
T31631 203-204 HYPH denotes -
T31632 208-215 JJ denotes Nuclear
T31633 216-219 CC denotes and
T31634 220-231 JJ denotes Cytoplasmic
T31635 232-242 NN denotes Extraction
T31636 247-248 -LRB- denotes (
T31637 248-255 NN denotes Product
T31638 255-256 SYM denotes #
T31639 257-262 CD denotes 78833
T31640 262-264 , denotes ,
T31641 271-284 NNP denotes Biotechnology
T31642 264-270 NNP denotes Pierce
T31643 284-286 , denotes ,
T31644 286-310 NN denotes http://www.piercenet.com
T31645 310-311 -RRB- denotes )
T31646 311-312 . denotes .
T31647 312-387 sentence denotes Proteins were separated by 10% SDS-PAGE and transferred to nitrocellulose.
T31648 313-321 NNS denotes Proteins
T31649 327-336 VBN denotes separated
T31650 322-326 VBD denotes were
T31651 337-339 IN denotes by
T31652 340-342 CD denotes 10
T31653 342-343 NN denotes %
T31654 348-352 NN denotes PAGE
T31655 344-347 NN denotes SDS
T31656 347-348 HYPH denotes -
T31657 353-356 CC denotes and
T31658 357-368 VBN denotes transferred
T31659 369-371 IN denotes to
T31660 372-386 NN denotes nitrocellulose
T31661 386-387 . denotes .
T31662 387-515 sentence denotes After blocking overnight at 4 °C in 5% skim milk, membranes were incubated in the primary antibody for 2 h at room temperature.
T31663 388-393 IN denotes After
T31664 453-462 VBN denotes incubated
T31665 394-402 VBG denotes blocking
T31666 403-412 RB denotes overnight
T31667 413-415 IN denotes at
T31668 416-417 CD denotes 4
T31669 418-420 NN denotes °C
T31670 421-423 IN denotes in
T31671 424-425 CD denotes 5
T31672 425-426 NN denotes %
T31673 432-436 NN denotes milk
T31674 427-431 NN denotes skim
T31675 436-438 , denotes ,
T31676 438-447 NNS denotes membranes
T31677 448-452 VBD denotes were
T31678 463-465 IN denotes in
T31679 466-469 DT denotes the
T31680 478-486 NN denotes antibody
T31681 470-477 JJ denotes primary
T31682 487-490 IN denotes for
T31683 491-492 CD denotes 2
T31684 493-494 NN denotes h
T31685 495-497 IN denotes at
T31686 498-502 NN denotes room
T31687 503-514 NN denotes temperature
T31688 514-515 . denotes .
T31689 515-795 sentence denotes After three 10-min washes in TPBS (100 mM Na2HPO4, 100 mM NaH2PO4, 0.5 N NaCl, 0.1% Tween-20), membranes were incubated for 30 min at room temperature in the secondary horseradish peroxidase-conjugated antibody (Jackson ImmunoResearch Laboratories, http://www.jacksonimmuno.com).
T31690 516-521 IN denotes After
T31691 626-635 VBN denotes incubated
T31692 522-527 CD denotes three
T31693 535-541 NNS denotes washes
T31694 528-530 CD denotes 10
T31695 531-534 NN denotes min
T31696 530-531 HYPH denotes -
T31697 542-544 IN denotes in
T31698 545-549 NN denotes TPBS
T31699 550-551 -LRB- denotes (
T31700 551-554 CD denotes 100
T31701 555-557 NN denotes mM
T31702 558-565 NN denotes Na2HPO4
T31703 565-567 , denotes ,
T31704 567-570 CD denotes 100
T31705 571-573 NN denotes mM
T31706 574-581 NN denotes NaH2PO4
T31707 581-583 , denotes ,
T31708 583-586 CD denotes 0.5
T31709 587-588 NN denotes N
T31710 589-593 NN denotes NaCl
T31711 593-595 , denotes ,
T31712 595-598 CD denotes 0.1
T31713 598-599 NN denotes %
T31714 600-605 NN denotes Tween
T31715 605-606 HYPH denotes -
T31716 606-608 CD denotes 20
T31717 608-609 -RRB- denotes )
T31718 609-611 , denotes ,
T31719 611-620 NNS denotes membranes
T31720 621-625 VBD denotes were
T31721 636-639 IN denotes for
T31722 640-642 CD denotes 30
T31723 643-646 NN denotes min
T31724 647-649 IN denotes at
T31725 650-654 NN denotes room
T31726 655-666 NN denotes temperature
T31727 667-669 IN denotes in
T31728 670-673 DT denotes the
T31729 718-726 NN denotes antibody
T31730 674-683 JJ denotes secondary
T31731 684-695 NN denotes horseradish
T31732 696-706 NN denotes peroxidase
T31733 707-717 VBN denotes conjugated
T31734 706-707 HYPH denotes -
T31735 727-728 -LRB- denotes (
T31736 751-763 NNP denotes Laboratories
T31737 728-735 NNP denotes Jackson
T31738 736-750 NNP denotes ImmunoResearch
T31739 763-765 , denotes ,
T31740 765-793 NN denotes http://www.jacksonimmuno.com
T31741 793-794 -RRB- denotes )
T31742 794-795 . denotes .
T31743 795-970 sentence denotes Blots were developed using the ECL-Plus chemiluminescent detection system (Amersham Pharmacia Biotech, http://www.pharmacia.ca), according to the manufacturer's instructions.
T31744 796-801 NNS denotes Blots
T31745 807-816 VBN denotes developed
T31746 802-806 VBD denotes were
T31747 817-822 VBG denotes using
T31748 823-826 DT denotes the
T31749 863-869 NN denotes system
T31750 827-830 NN denotes ECL
T31751 831-835 NN denotes Plus
T31752 830-831 HYPH denotes -
T31753 836-852 JJ denotes chemiluminescent
T31754 853-862 NN denotes detection
T31755 870-871 -LRB- denotes (
T31756 890-897 NNP denotes Biotech
T31757 871-879 NNP denotes Amersham
T31758 880-889 NNP denotes Pharmacia
T31759 897-899 , denotes ,
T31760 899-922 NN denotes http://www.pharmacia.ca
T31761 922-923 -RRB- denotes )
T31762 923-925 , denotes ,
T31763 925-934 VBG denotes according
T31764 935-937 IN denotes to
T31765 938-941 DT denotes the
T31766 942-954 NN denotes manufacturer
T31767 957-969 NNS denotes instructions
T31768 954-956 POS denotes 's
T31769 969-970 . denotes .
T31770 970-1330 sentence denotes The following primary antibodies were used: E2f-1 (SC-193), E2f-3 (SC-878), Cdkn1a (p21, SC-471), Cdkn1b (p27, SC-528), Pou4f2 (Brn3b, SC-6062), and Tfdp1 (Dp1, SC-610) from Santa Cruz Biotechnology (http://www.scbt.com), pRB (554136) from BD Science-Pharmingen (http://www.bdbiosciences.com), and Slc18a3 (VAChT, G448A) from Promega (http://www.promega.com).
T31771 971-974 DT denotes The
T31772 993-1003 NNS denotes antibodies
T31773 975-984 VBG denotes following
T31774 985-992 JJ denotes primary
T31775 1009-1013 VBN denotes used
T31776 1004-1008 VBD denotes were
T31777 1013-1015 : denotes :
T31778 1015-1018 NN denotes E2f
T31779 1018-1019 HYPH denotes -
T31780 1019-1020 CD denotes 1
T31781 1021-1022 -LRB- denotes (
T31782 1022-1024 NN denotes SC
T31783 1024-1025 HYPH denotes -
T31784 1025-1028 CD denotes 193
T31785 1028-1029 -RRB- denotes )
T31786 1029-1031 , denotes ,
T31787 1031-1034 NN denotes E2f
T31788 1034-1035 HYPH denotes -
T31789 1035-1036 CD denotes 3
T31790 1037-1038 -LRB- denotes (
T31791 1038-1040 NN denotes SC
T31792 1040-1041 HYPH denotes -
T31793 1041-1044 CD denotes 878
T31794 1044-1045 -RRB- denotes )
T31795 1045-1047 , denotes ,
T31796 1047-1053 NN denotes Cdkn1a
T31797 1054-1055 -LRB- denotes (
T31798 1055-1058 NN denotes p21
T31799 1058-1060 , denotes ,
T31800 1060-1062 NN denotes SC
T31801 1062-1063 HYPH denotes -
T31802 1063-1066 CD denotes 471
T31803 1066-1067 -RRB- denotes )
T31804 1067-1069 , denotes ,
T31805 1069-1075 NN denotes Cdkn1b
T31806 1076-1077 -LRB- denotes (
T31807 1077-1080 NN denotes p27
T31808 1080-1082 , denotes ,
T31809 1082-1084 NN denotes SC
T31810 1084-1085 HYPH denotes -
T31811 1085-1088 CD denotes 528
T31812 1088-1089 -RRB- denotes )
T31813 1089-1091 , denotes ,
T31814 1091-1097 NN denotes Pou4f2
T31815 1098-1099 -LRB- denotes (
T31816 1099-1104 NN denotes Brn3b
T31817 1104-1106 , denotes ,
T31818 1106-1108 NN denotes SC
T31819 1108-1109 HYPH denotes -
T31820 1109-1113 CD denotes 6062
T31821 1113-1114 -RRB- denotes )
T31822 1114-1116 , denotes ,
T31823 1116-1119 CC denotes and
T31824 1120-1125 NN denotes Tfdp1
T31825 1126-1127 -LRB- denotes (
T31826 1127-1130 NN denotes Dp1
T31827 1130-1132 , denotes ,
T31828 1132-1134 NN denotes SC
T31829 1134-1135 HYPH denotes -
T31830 1135-1138 CD denotes 610
T31831 1138-1139 -RRB- denotes )
T31832 1140-1144 IN denotes from
T31833 1145-1150 NNP denotes Santa
T31834 1156-1169 NNP denotes Biotechnology
T31835 1151-1155 NNP denotes Cruz
T31836 1170-1171 -LRB- denotes (
T31837 1171-1190 NN denotes http://www.scbt.com
T31838 1190-1191 -RRB- denotes )
T31839 1191-1193 , denotes ,
T31840 1193-1196 NN denotes pRB
T31841 1197-1198 -LRB- denotes (
T31842 1198-1204 CD denotes 554136
T31843 1204-1205 -RRB- denotes )
T31844 1206-1210 IN denotes from
T31845 1211-1213 NNP denotes BD
T31846 1222-1232 NNP denotes Pharmingen
T31847 1214-1221 NNP denotes Science
T31848 1221-1222 HYPH denotes -
T31849 1233-1234 -LRB- denotes (
T31850 1234-1262 NN denotes http://www.bdbiosciences.com
T31851 1262-1263 -RRB- denotes )
T31852 1263-1265 , denotes ,
T31853 1265-1268 CC denotes and
T31854 1269-1276 NN denotes Slc18a3
T31855 1277-1278 -LRB- denotes (
T31856 1278-1283 NN denotes VAChT
T31857 1283-1285 , denotes ,
T31858 1285-1290 NN denotes G448A
T31859 1290-1291 -RRB- denotes )
T31860 1292-1296 IN denotes from
T31861 1297-1304 NNP denotes Promega
T31862 1305-1306 -LRB- denotes (
T31863 1306-1328 NN denotes http://www.promega.com
T31864 1328-1329 -RRB- denotes )
T31865 1329-1330 . denotes .
R9426 T31588 T31589 compound Western,blots
R9427 T31590 T31589 punct .,blots
R9428 T31592 T31593 compound Mouse,retinas
R9429 T31593 T31594 nsubjpass retinas,homogenized
R9430 T31595 T31594 auxpass were,homogenized
R9431 T31596 T31594 prep by,homogenized
R9432 T31597 T31596 pcomp passing,by
R9433 T31598 T31597 dobj them,passing
R9434 T31599 T31597 prep through,passing
R9435 T31600 T31601 det a,needle
R9436 T31601 T31599 pobj needle,through
R9437 T31602 T31603 nummod 30,gauge
R9438 T31603 T31601 nmod gauge,needle
R9439 T31604 T31603 punct -,gauge
R9440 T31605 T31601 nmod BD,needle
R9441 T31606 T31605 nummod 9,BD
R9442 T31607 T31605 appos http://www.bd.com,BD
R9443 T31608 T31601 punct ),needle
R9444 T31609 T31610 quantmod 5,10
R9445 T31610 T31612 nummod 10,times
R9446 T31611 T31610 punct –,10
R9447 T31612 T31594 npadvmod times,homogenized
R9448 T31613 T31594 prep in,homogenized
R9449 T31614 T31615 nummod 1,solution
R9450 T31615 T31613 pobj solution,in
R9451 T31616 T31614 punct ×,1
R9452 T31617 T31615 compound PBS,solution
R9453 T31618 T31594 punct .,homogenized
R9454 T31620 T31621 amod Nuclear,proteins
R9455 T31621 T31624 nsubjpass proteins,extracted
R9456 T31622 T31620 cc and,Nuclear
R9457 T31623 T31620 conj cytoplasmic,Nuclear
R9458 T31625 T31624 auxpass were,extracted
R9459 T31626 T31624 advcl using,extracted
R9460 T31627 T31628 det the,Kit
R9461 T31628 T31626 dobj Kit,using
R9462 T31629 T31630 nmod NE,PER
R9463 T31630 T31628 nmod PER,Kit
R9464 T31631 T31630 punct -,PER
R9465 T31632 T31628 amod Nuclear,Kit
R9466 T31633 T31632 cc and,Nuclear
R9467 T31634 T31632 conj Cytoplasmic,Nuclear
R9468 T31635 T31628 compound Extraction,Kit
R9469 T31636 T31628 punct (,Kit
R9470 T31637 T31628 appos Product,Kit
R9471 T31638 T31637 punct #,Product
R9472 T31639 T31637 nummod 78833,Product
R9473 T31640 T31641 punct ", ",Biotechnology
R9474 T31641 T31628 parataxis Biotechnology,Kit
R9475 T31642 T31641 compound Pierce,Biotechnology
R9476 T31643 T31641 punct ", ",Biotechnology
R9477 T31644 T31641 npadvmod http://www.piercenet.com,Biotechnology
R9478 T31645 T31641 punct ),Biotechnology
R9479 T31646 T31624 punct .,extracted
R9480 T31648 T31649 nsubjpass Proteins,separated
R9481 T31650 T31649 auxpass were,separated
R9482 T31651 T31649 prep by,separated
R9483 T31652 T31653 nummod 10,%
R9484 T31653 T31654 compound %,PAGE
R9485 T31654 T31651 pobj PAGE,by
R9486 T31655 T31654 compound SDS,PAGE
R9487 T31656 T31654 punct -,PAGE
R9488 T31657 T31649 cc and,separated
R9489 T31658 T31649 conj transferred,separated
R9490 T31659 T31658 prep to,transferred
R9491 T31660 T31659 pobj nitrocellulose,to
R9492 T31661 T31649 punct .,separated
R9493 T31663 T31664 prep After,incubated
R9494 T31665 T31663 pcomp blocking,After
R9495 T31666 T31665 advmod overnight,blocking
R9496 T31667 T31665 prep at,blocking
R9497 T31668 T31669 nummod 4,°C
R9498 T31669 T31667 pobj °C,at
R9499 T31670 T31665 prep in,blocking
R9500 T31671 T31672 nummod 5,%
R9501 T31672 T31673 compound %,milk
R9502 T31673 T31670 pobj milk,in
R9503 T31674 T31673 compound skim,milk
R9504 T31675 T31664 punct ", ",incubated
R9505 T31676 T31664 nsubjpass membranes,incubated
R9506 T31677 T31664 auxpass were,incubated
R9507 T31678 T31664 prep in,incubated
R9508 T31679 T31680 det the,antibody
R9509 T31680 T31678 pobj antibody,in
R9510 T31681 T31680 amod primary,antibody
R9511 T31682 T31664 prep for,incubated
R9512 T31683 T31684 nummod 2,h
R9513 T31684 T31682 pobj h,for
R9514 T31685 T31664 prep at,incubated
R9515 T31686 T31687 compound room,temperature
R9516 T31687 T31685 pobj temperature,at
R9517 T31688 T31664 punct .,incubated
R9518 T31690 T31691 prep After,incubated
R9519 T31692 T31693 nummod three,washes
R9520 T31693 T31690 pobj washes,After
R9521 T31694 T31695 nummod 10,min
R9522 T31695 T31693 compound min,washes
R9523 T31696 T31695 punct -,min
R9524 T31697 T31693 prep in,washes
R9525 T31698 T31697 pobj TPBS,in
R9526 T31699 T31698 punct (,TPBS
R9527 T31700 T31701 nummod 100,mM
R9528 T31701 T31702 compound mM,Na2HPO4
R9529 T31702 T31698 appos Na2HPO4,TPBS
R9530 T31703 T31702 punct ", ",Na2HPO4
R9531 T31704 T31705 nummod 100,mM
R9532 T31705 T31706 compound mM,NaH2PO4
R9533 T31706 T31702 appos NaH2PO4,Na2HPO4
R9534 T31707 T31702 punct ", ",Na2HPO4
R9535 T31708 T31709 nummod 0.5,N
R9536 T31709 T31710 compound N,NaCl
R9537 T31710 T31702 appos NaCl,Na2HPO4
R9538 T31711 T31702 punct ", ",Na2HPO4
R9539 T31712 T31713 nummod 0.1,%
R9540 T31713 T31714 compound %,Tween
R9541 T31714 T31702 appos Tween,Na2HPO4
R9542 T31715 T31714 punct -,Tween
R9543 T31716 T31714 nummod 20,Tween
R9544 T31717 T31691 punct ),incubated
R9545 T31718 T31691 punct ", ",incubated
R9546 T31719 T31691 nsubjpass membranes,incubated
R9547 T31720 T31691 auxpass were,incubated
R9548 T31721 T31691 prep for,incubated
R9549 T31722 T31723 nummod 30,min
R9550 T31723 T31721 pobj min,for
R9551 T31724 T31691 prep at,incubated
R9552 T31725 T31726 compound room,temperature
R9553 T31726 T31724 pobj temperature,at
R9554 T31727 T31691 prep in,incubated
R9555 T31728 T31729 det the,antibody
R9556 T31729 T31727 pobj antibody,in
R9557 T31730 T31729 amod secondary,antibody
R9558 T31731 T31732 compound horseradish,peroxidase
R9559 T31732 T31733 npadvmod peroxidase,conjugated
R9560 T31733 T31729 amod conjugated,antibody
R9561 T31734 T31733 punct -,conjugated
R9562 T31735 T31736 punct (,Laboratories
R9563 T31736 T31729 parataxis Laboratories,antibody
R9564 T31737 T31736 compound Jackson,Laboratories
R9565 T31738 T31736 compound ImmunoResearch,Laboratories
R9566 T31739 T31736 punct ", ",Laboratories
R9567 T31740 T31736 npadvmod http://www.jacksonimmuno.com,Laboratories
R9568 T31741 T31736 punct ),Laboratories
R9569 T31742 T31691 punct .,incubated
R9570 T31744 T31745 nsubjpass Blots,developed
R9571 T31746 T31745 auxpass were,developed
R9572 T31747 T31745 advcl using,developed
R9573 T31748 T31749 det the,system
R9574 T31749 T31747 dobj system,using
R9575 T31750 T31751 nmod ECL,Plus
R9576 T31751 T31749 nmod Plus,system
R9577 T31752 T31751 punct -,Plus
R9578 T31753 T31749 amod chemiluminescent,system
R9579 T31754 T31749 compound detection,system
R9580 T31755 T31756 punct (,Biotech
R9581 T31756 T31749 parataxis Biotech,system
R9582 T31757 T31756 compound Amersham,Biotech
R9583 T31758 T31756 compound Pharmacia,Biotech
R9584 T31759 T31756 punct ", ",Biotech
R9585 T31760 T31756 npadvmod http://www.pharmacia.ca,Biotech
R9586 T31761 T31756 punct ),Biotech
R9587 T31762 T31747 punct ", ",using
R9588 T31763 T31747 prep according,using
R9589 T31764 T31763 prep to,according
R9590 T31765 T31766 det the,manufacturer
R9591 T31766 T31767 poss manufacturer,instructions
R9592 T31767 T31764 pobj instructions,to
R9593 T31768 T31766 case 's,manufacturer
R9594 T31769 T31745 punct .,developed
R9595 T31771 T31772 det The,antibodies
R9596 T31772 T31775 nsubjpass antibodies,used
R9597 T31773 T31772 amod following,antibodies
R9598 T31774 T31772 amod primary,antibodies
R9599 T31776 T31775 auxpass were,used
R9600 T31777 T31775 punct : ,used
R9601 T31778 T31775 dobj E2f,used
R9602 T31779 T31778 punct -,E2f
R9603 T31780 T31778 nummod 1,E2f
R9604 T31781 T31778 punct (,E2f
R9605 T31782 T31778 appos SC,E2f
R9606 T31783 T31782 punct -,SC
R9607 T31784 T31782 nummod 193,SC
R9608 T31785 T31778 punct ),E2f
R9609 T31786 T31778 punct ", ",E2f
R9610 T31787 T31778 conj E2f,E2f
R9611 T31788 T31787 punct -,E2f
R9612 T31789 T31787 nummod 3,E2f
R9613 T31790 T31787 punct (,E2f
R9614 T31791 T31787 appos SC,E2f
R9615 T31792 T31791 punct -,SC
R9616 T31793 T31791 nummod 878,SC
R9617 T31794 T31787 punct ),E2f
R9618 T31795 T31787 punct ", ",E2f
R9619 T31796 T31787 conj Cdkn1a,E2f
R9620 T31797 T31796 punct (,Cdkn1a
R9621 T31798 T31796 appos p21,Cdkn1a
R9622 T31799 T31796 punct ", ",Cdkn1a
R9623 T31800 T31796 appos SC,Cdkn1a
R9624 T31801 T31800 punct -,SC
R9625 T31802 T31800 nummod 471,SC
R9626 T31803 T31796 punct ),Cdkn1a
R9627 T31804 T31796 punct ", ",Cdkn1a
R9628 T31805 T31796 conj Cdkn1b,Cdkn1a
R9629 T31806 T31805 punct (,Cdkn1b
R9630 T31807 T31805 appos p27,Cdkn1b
R9631 T31808 T31805 punct ", ",Cdkn1b
R9632 T31809 T31805 appos SC,Cdkn1b
R9633 T31810 T31809 punct -,SC
R9634 T31811 T31809 nummod 528,SC
R9635 T31812 T31805 punct ),Cdkn1b
R9636 T31813 T31805 punct ", ",Cdkn1b
R9637 T31814 T31805 conj Pou4f2,Cdkn1b
R9638 T31815 T31814 punct (,Pou4f2
R9639 T31816 T31814 appos Brn3b,Pou4f2
R9640 T31817 T31814 punct ", ",Pou4f2
R9641 T31818 T31814 appos SC,Pou4f2
R9642 T31819 T31818 punct -,SC
R9643 T31820 T31818 nummod 6062,SC
R9644 T31821 T31814 punct ),Pou4f2
R9645 T31822 T31814 punct ", ",Pou4f2
R9646 T31823 T31814 cc and,Pou4f2
R9647 T31824 T31814 conj Tfdp1,Pou4f2
R9648 T31825 T31824 punct (,Tfdp1
R9649 T31826 T31824 appos Dp1,Tfdp1
R9650 T31827 T31824 punct ", ",Tfdp1
R9651 T31828 T31824 appos SC,Tfdp1
R9652 T31829 T31828 punct -,SC
R9653 T31830 T31828 nummod 610,SC
R9654 T31831 T31778 punct ),E2f
R9655 T31832 T31778 prep from,E2f
R9656 T31833 T31834 compound Santa,Biotechnology
R9657 T31834 T31832 pobj Biotechnology,from
R9658 T31835 T31834 compound Cruz,Biotechnology
R9659 T31836 T31834 punct (,Biotechnology
R9660 T31837 T31834 npadvmod http://www.scbt.com,Biotechnology
R9661 T31838 T31778 punct ),E2f
R9662 T31839 T31778 punct ", ",E2f
R9663 T31840 T31778 conj pRB,E2f
R9664 T31841 T31840 punct (,pRB
R9665 T31842 T31840 appos 554136,pRB
R9666 T31843 T31840 punct ),pRB
R9667 T31844 T31840 prep from,pRB
R9668 T31845 T31846 compound BD,Pharmingen
R9669 T31846 T31844 pobj Pharmingen,from
R9670 T31847 T31846 compound Science,Pharmingen
R9671 T31848 T31846 punct -,Pharmingen
R9672 T31849 T31846 punct (,Pharmingen
R9673 T31850 T31846 npadvmod http://www.bdbiosciences.com,Pharmingen
R9674 T31851 T31840 punct ),pRB
R9675 T31852 T31840 punct ", ",pRB
R9676 T31853 T31840 cc and,pRB
R9677 T31854 T31840 conj Slc18a3,pRB
R9678 T31855 T31854 punct (,Slc18a3
R9679 T31856 T31854 appos VAChT,Slc18a3
R9680 T31857 T31854 punct ", ",Slc18a3
R9681 T31858 T31854 appos G448A,Slc18a3
R9682 T31859 T31854 punct ),Slc18a3
R9683 T31860 T31854 prep from,Slc18a3
R9684 T31861 T31860 pobj Promega,from
R9685 T31862 T31861 punct (,Promega
R9686 T31863 T31861 npadvmod http://www.promega.com,Promega
R9687 T31864 T31775 punct ),used
R9688 T31865 T31775 punct .,used

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T31552 15-20 NCBITaxon:10088 denotes Mouse
T31553 21-28 UBERON:0000966 denotes retinas
T31554 49-56 GO_EXT:biological_movement_or_translocation_process denotes passing
T31555 133-141 CHEBI:75958 denotes solution
T31556 143-150 GO:0005634 denotes Nuclear
T31557 155-166 GO:0005737 denotes cytoplasmic
T31558 167-175 CHEBI_PR_EXT:protein denotes proteins
T31559 208-215 GO:0005634 denotes Nuclear
T31560 220-231 GO:0005737 denotes Cytoplasmic
T31561 313-321 CHEBI_PR_EXT:protein denotes Proteins
T31562 344-347 CHEBI:8984 denotes SDS
T31563 357-368 GO_EXT:biological_movement_or_translocation_process denotes transferred
T31564 372-386 CHEBI:53325 denotes nitrocellulose
T31565 432-436 UBERON:0001913 denotes milk
T31566 478-486 GO:0042571 denotes antibody
T31567 558-565 CHEBI:34683 denotes Na2HPO4
T31568 574-581 CHEBI:37585 denotes NaH2PO4
T31569 589-593 CHEBI:26710 denotes NaCl
T31570 600-608 CHEBI:53424 denotes Tween-20
T31571 684-695 NCBITaxon:3704 denotes horseradish
T31572 696-706 GO_EXT:0004601 denotes peroxidase
T31573 707-717 MOP:0000779 denotes conjugated
T31574 718-726 GO:0042571 denotes antibody
T31575 993-1003 GO:0042571 denotes antibodies
T31576 1015-1020 PR_EXT:000006852 denotes E2f-1
T31577 1031-1036 PR_EXT:000006854 denotes E2f-3
T31578 1047-1053 PR_EXT:000005274 denotes Cdkn1a
T31579 1055-1058 PR_EXT:000005274 denotes p21
T31580 1069-1075 PR_EXT:000003090 denotes Cdkn1b
T31581 1077-1080 PR_EXT:000003090 denotes p27
T31582 1091-1097 PR_EXT:000013042 denotes Pou4f2
T31583 1099-1104 PR_EXT:000013042 denotes Brn3b
T31584 1120-1125 PR_EXT:000016269 denotes Tfdp1
T31585 1127-1130 PR_EXT:000016269 denotes Dp1
T31586 1269-1276 PR_EXT:000014968 denotes Slc18a3
T31587 1278-1283 PR_EXT:000014968 denotes VAChT

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T31521 15-20 NCBITaxon:10088 denotes Mouse
T31522 21-28 UBERON:0000966 denotes retinas
T31523 133-141 CHEBI:75958 denotes solution
T31524 143-150 GO:0005634 denotes Nuclear
T31525 155-166 GO:0005737 denotes cytoplasmic
T31526 208-215 GO:0005634 denotes Nuclear
T31527 220-231 GO:0005737 denotes Cytoplasmic
T31528 344-347 CHEBI:8984 denotes SDS
T31529 372-386 CHEBI:53325 denotes nitrocellulose
T31530 432-436 UBERON:0001913 denotes milk
T31531 478-486 GO:0042571 denotes antibody
T31532 558-565 CHEBI:34683 denotes Na2HPO4
T31533 574-581 CHEBI:37585 denotes NaH2PO4
T31534 589-593 CHEBI:26710 denotes NaCl
T31535 600-608 CHEBI:53424 denotes Tween-20
T31536 684-695 NCBITaxon:3704 denotes horseradish
T31537 707-717 MOP:0000779 denotes conjugated
T31538 718-726 GO:0042571 denotes antibody
T31539 993-1003 GO:0042571 denotes antibodies
T31540 1015-1020 PR:000006852 denotes E2f-1
T31541 1031-1036 PR:000006854 denotes E2f-3
T31542 1047-1053 PR:000005274 denotes Cdkn1a
T31543 1055-1058 PR:000005274 denotes p21
T31544 1069-1075 PR:000003090 denotes Cdkn1b
T31545 1077-1080 PR:000003090 denotes p27
T31546 1091-1097 PR:000013042 denotes Pou4f2
T31547 1099-1104 PR:000013042 denotes Brn3b
T31548 1120-1125 PR:000016269 denotes Tfdp1
T31549 1127-1130 PR:000016269 denotes Dp1
T31550 1269-1276 PR:000014968 denotes Slc18a3
T31551 1278-1283 PR:000014968 denotes VAChT