Id |
Subject |
Object |
Predicate |
Lexical cue |
T31605 |
81-83 |
NN |
denotes |
BD |
T31606 |
84-85 |
CD |
denotes |
9 |
T31588 |
0-7 |
NNP |
denotes |
Western |
T31589 |
8-13 |
NNS |
denotes |
blots |
T31590 |
13-14 |
. |
denotes |
. |
T31591 |
14-142 |
sentence |
denotes |
Mouse retinas were homogenized by passing them through a 30-gauge BD 9 http://www.bd.com) needle 5–10 times in 1× PBS solution. |
T31592 |
15-20 |
NN |
denotes |
Mouse |
T31593 |
21-28 |
NNS |
denotes |
retinas |
T31594 |
34-45 |
VBN |
denotes |
homogenized |
T31595 |
29-33 |
VBD |
denotes |
were |
T31596 |
46-48 |
IN |
denotes |
by |
T31597 |
49-56 |
VBG |
denotes |
passing |
T31598 |
57-61 |
PRP |
denotes |
them |
T31599 |
62-69 |
IN |
denotes |
through |
T31600 |
70-71 |
DT |
denotes |
a |
T31601 |
105-111 |
NN |
denotes |
needle |
T31602 |
72-74 |
CD |
denotes |
30 |
T31603 |
75-80 |
NN |
denotes |
gauge |
T31604 |
74-75 |
HYPH |
denotes |
- |
T31607 |
86-103 |
NN |
denotes |
http://www.bd.com |
T31608 |
103-104 |
-RRB- |
denotes |
) |
T31609 |
112-113 |
CD |
denotes |
5 |
T31610 |
114-116 |
CD |
denotes |
10 |
T31611 |
113-114 |
SYM |
denotes |
– |
T31612 |
117-122 |
NNS |
denotes |
times |
T31613 |
123-125 |
IN |
denotes |
in |
T31614 |
126-127 |
CD |
denotes |
1 |
T31615 |
133-141 |
NN |
denotes |
solution |
T31616 |
127-128 |
SYM |
denotes |
× |
T31617 |
129-132 |
NN |
denotes |
PBS |
T31618 |
141-142 |
. |
denotes |
. |
T31619 |
142-312 |
sentence |
denotes |
Nuclear and cytoplasmic proteins were extracted using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Product# 78833, Pierce Biotechnology, http://www.piercenet.com). |
T31620 |
143-150 |
JJ |
denotes |
Nuclear |
T31621 |
167-175 |
NN |
denotes |
proteins |
T31622 |
151-154 |
CC |
denotes |
and |
T31623 |
155-166 |
JJ |
denotes |
cytoplasmic |
T31624 |
181-190 |
VBN |
denotes |
extracted |
T31625 |
176-180 |
VBD |
denotes |
were |
T31626 |
191-196 |
VBG |
denotes |
using |
T31627 |
197-200 |
DT |
denotes |
the |
T31628 |
243-246 |
NN |
denotes |
Kit |
T31629 |
201-203 |
NN |
denotes |
NE |
T31630 |
204-207 |
NN |
denotes |
PER |
T31631 |
203-204 |
HYPH |
denotes |
- |
T31632 |
208-215 |
JJ |
denotes |
Nuclear |
T31633 |
216-219 |
CC |
denotes |
and |
T31634 |
220-231 |
JJ |
denotes |
Cytoplasmic |
T31635 |
232-242 |
NN |
denotes |
Extraction |
T31636 |
247-248 |
-LRB- |
denotes |
( |
T31637 |
248-255 |
NN |
denotes |
Product |
T31638 |
255-256 |
SYM |
denotes |
# |
T31639 |
257-262 |
CD |
denotes |
78833 |
T31640 |
262-264 |
, |
denotes |
, |
T31641 |
271-284 |
NNP |
denotes |
Biotechnology |
T31642 |
264-270 |
NNP |
denotes |
Pierce |
T31643 |
284-286 |
, |
denotes |
, |
T31644 |
286-310 |
NN |
denotes |
http://www.piercenet.com |
T31645 |
310-311 |
-RRB- |
denotes |
) |
T31646 |
311-312 |
. |
denotes |
. |
T31647 |
312-387 |
sentence |
denotes |
Proteins were separated by 10% SDS-PAGE and transferred to nitrocellulose. |
T31648 |
313-321 |
NNS |
denotes |
Proteins |
T31649 |
327-336 |
VBN |
denotes |
separated |
T31650 |
322-326 |
VBD |
denotes |
were |
T31651 |
337-339 |
IN |
denotes |
by |
T31652 |
340-342 |
CD |
denotes |
10 |
T31653 |
342-343 |
NN |
denotes |
% |
T31654 |
348-352 |
NN |
denotes |
PAGE |
T31655 |
344-347 |
NN |
denotes |
SDS |
T31656 |
347-348 |
HYPH |
denotes |
- |
T31657 |
353-356 |
CC |
denotes |
and |
T31658 |
357-368 |
VBN |
denotes |
transferred |
T31659 |
369-371 |
IN |
denotes |
to |
T31660 |
372-386 |
NN |
denotes |
nitrocellulose |
T31661 |
386-387 |
. |
denotes |
. |
T31662 |
387-515 |
sentence |
denotes |
After blocking overnight at 4 °C in 5% skim milk, membranes were incubated in the primary antibody for 2 h at room temperature. |
T31663 |
388-393 |
IN |
denotes |
After |
T31664 |
453-462 |
VBN |
denotes |
incubated |
T31665 |
394-402 |
VBG |
denotes |
blocking |
T31666 |
403-412 |
RB |
denotes |
overnight |
T31667 |
413-415 |
IN |
denotes |
at |
T31668 |
416-417 |
CD |
denotes |
4 |
T31669 |
418-420 |
NN |
denotes |
°C |
T31670 |
421-423 |
IN |
denotes |
in |
T31671 |
424-425 |
CD |
denotes |
5 |
T31672 |
425-426 |
NN |
denotes |
% |
T31673 |
432-436 |
NN |
denotes |
milk |
T31674 |
427-431 |
NN |
denotes |
skim |
T31675 |
436-438 |
, |
denotes |
, |
T31676 |
438-447 |
NNS |
denotes |
membranes |
T31677 |
448-452 |
VBD |
denotes |
were |
T31678 |
463-465 |
IN |
denotes |
in |
T31679 |
466-469 |
DT |
denotes |
the |
T31680 |
478-486 |
NN |
denotes |
antibody |
T31681 |
470-477 |
JJ |
denotes |
primary |
T31682 |
487-490 |
IN |
denotes |
for |
T31683 |
491-492 |
CD |
denotes |
2 |
T31684 |
493-494 |
NN |
denotes |
h |
T31685 |
495-497 |
IN |
denotes |
at |
T31686 |
498-502 |
NN |
denotes |
room |
T31687 |
503-514 |
NN |
denotes |
temperature |
T31688 |
514-515 |
. |
denotes |
. |
T31689 |
515-795 |
sentence |
denotes |
After three 10-min washes in TPBS (100 mM Na2HPO4, 100 mM NaH2PO4, 0.5 N NaCl, 0.1% Tween-20), membranes were incubated for 30 min at room temperature in the secondary horseradish peroxidase-conjugated antibody (Jackson ImmunoResearch Laboratories, http://www.jacksonimmuno.com). |
T31690 |
516-521 |
IN |
denotes |
After |
T31691 |
626-635 |
VBN |
denotes |
incubated |
T31692 |
522-527 |
CD |
denotes |
three |
T31693 |
535-541 |
NNS |
denotes |
washes |
T31694 |
528-530 |
CD |
denotes |
10 |
T31695 |
531-534 |
NN |
denotes |
min |
T31696 |
530-531 |
HYPH |
denotes |
- |
T31697 |
542-544 |
IN |
denotes |
in |
T31698 |
545-549 |
NN |
denotes |
TPBS |
T31699 |
550-551 |
-LRB- |
denotes |
( |
T31700 |
551-554 |
CD |
denotes |
100 |
T31701 |
555-557 |
NN |
denotes |
mM |
T31702 |
558-565 |
NN |
denotes |
Na2HPO4 |
T31703 |
565-567 |
, |
denotes |
, |
T31704 |
567-570 |
CD |
denotes |
100 |
T31705 |
571-573 |
NN |
denotes |
mM |
T31706 |
574-581 |
NN |
denotes |
NaH2PO4 |
T31707 |
581-583 |
, |
denotes |
, |
T31708 |
583-586 |
CD |
denotes |
0.5 |
T31709 |
587-588 |
NN |
denotes |
N |
T31710 |
589-593 |
NN |
denotes |
NaCl |
T31711 |
593-595 |
, |
denotes |
, |
T31712 |
595-598 |
CD |
denotes |
0.1 |
T31713 |
598-599 |
NN |
denotes |
% |
T31714 |
600-605 |
NN |
denotes |
Tween |
T31715 |
605-606 |
HYPH |
denotes |
- |
T31716 |
606-608 |
CD |
denotes |
20 |
T31717 |
608-609 |
-RRB- |
denotes |
) |
T31718 |
609-611 |
, |
denotes |
, |
T31719 |
611-620 |
NNS |
denotes |
membranes |
T31720 |
621-625 |
VBD |
denotes |
were |
T31721 |
636-639 |
IN |
denotes |
for |
T31722 |
640-642 |
CD |
denotes |
30 |
T31723 |
643-646 |
NN |
denotes |
min |
T31724 |
647-649 |
IN |
denotes |
at |
T31725 |
650-654 |
NN |
denotes |
room |
T31726 |
655-666 |
NN |
denotes |
temperature |
T31727 |
667-669 |
IN |
denotes |
in |
T31728 |
670-673 |
DT |
denotes |
the |
T31729 |
718-726 |
NN |
denotes |
antibody |
T31730 |
674-683 |
JJ |
denotes |
secondary |
T31731 |
684-695 |
NN |
denotes |
horseradish |
T31732 |
696-706 |
NN |
denotes |
peroxidase |
T31733 |
707-717 |
VBN |
denotes |
conjugated |
T31734 |
706-707 |
HYPH |
denotes |
- |
T31735 |
727-728 |
-LRB- |
denotes |
( |
T31736 |
751-763 |
NNP |
denotes |
Laboratories |
T31737 |
728-735 |
NNP |
denotes |
Jackson |
T31738 |
736-750 |
NNP |
denotes |
ImmunoResearch |
T31739 |
763-765 |
, |
denotes |
, |
T31740 |
765-793 |
NN |
denotes |
http://www.jacksonimmuno.com |
T31741 |
793-794 |
-RRB- |
denotes |
) |
T31742 |
794-795 |
. |
denotes |
. |
T31743 |
795-970 |
sentence |
denotes |
Blots were developed using the ECL-Plus chemiluminescent detection system (Amersham Pharmacia Biotech, http://www.pharmacia.ca), according to the manufacturer's instructions. |
T31744 |
796-801 |
NNS |
denotes |
Blots |
T31745 |
807-816 |
VBN |
denotes |
developed |
T31746 |
802-806 |
VBD |
denotes |
were |
T31747 |
817-822 |
VBG |
denotes |
using |
T31748 |
823-826 |
DT |
denotes |
the |
T31749 |
863-869 |
NN |
denotes |
system |
T31750 |
827-830 |
NN |
denotes |
ECL |
T31751 |
831-835 |
NN |
denotes |
Plus |
T31752 |
830-831 |
HYPH |
denotes |
- |
T31753 |
836-852 |
JJ |
denotes |
chemiluminescent |
T31754 |
853-862 |
NN |
denotes |
detection |
T31755 |
870-871 |
-LRB- |
denotes |
( |
T31756 |
890-897 |
NNP |
denotes |
Biotech |
T31757 |
871-879 |
NNP |
denotes |
Amersham |
T31758 |
880-889 |
NNP |
denotes |
Pharmacia |
T31759 |
897-899 |
, |
denotes |
, |
T31760 |
899-922 |
NN |
denotes |
http://www.pharmacia.ca |
T31761 |
922-923 |
-RRB- |
denotes |
) |
T31762 |
923-925 |
, |
denotes |
, |
T31763 |
925-934 |
VBG |
denotes |
according |
T31764 |
935-937 |
IN |
denotes |
to |
T31765 |
938-941 |
DT |
denotes |
the |
T31766 |
942-954 |
NN |
denotes |
manufacturer |
T31767 |
957-969 |
NNS |
denotes |
instructions |
T31768 |
954-956 |
POS |
denotes |
's |
T31769 |
969-970 |
. |
denotes |
. |
T31770 |
970-1330 |
sentence |
denotes |
The following primary antibodies were used: E2f-1 (SC-193), E2f-3 (SC-878), Cdkn1a (p21, SC-471), Cdkn1b (p27, SC-528), Pou4f2 (Brn3b, SC-6062), and Tfdp1 (Dp1, SC-610) from Santa Cruz Biotechnology (http://www.scbt.com), pRB (554136) from BD Science-Pharmingen (http://www.bdbiosciences.com), and Slc18a3 (VAChT, G448A) from Promega (http://www.promega.com). |
T31771 |
971-974 |
DT |
denotes |
The |
T31772 |
993-1003 |
NNS |
denotes |
antibodies |
T31773 |
975-984 |
VBG |
denotes |
following |
T31774 |
985-992 |
JJ |
denotes |
primary |
T31775 |
1009-1013 |
VBN |
denotes |
used |
T31776 |
1004-1008 |
VBD |
denotes |
were |
T31777 |
1013-1015 |
: |
denotes |
: |
T31778 |
1015-1018 |
NN |
denotes |
E2f |
T31779 |
1018-1019 |
HYPH |
denotes |
- |
T31780 |
1019-1020 |
CD |
denotes |
1 |
T31781 |
1021-1022 |
-LRB- |
denotes |
( |
T31782 |
1022-1024 |
NN |
denotes |
SC |
T31783 |
1024-1025 |
HYPH |
denotes |
- |
T31784 |
1025-1028 |
CD |
denotes |
193 |
T31785 |
1028-1029 |
-RRB- |
denotes |
) |
T31786 |
1029-1031 |
, |
denotes |
, |
T31787 |
1031-1034 |
NN |
denotes |
E2f |
T31788 |
1034-1035 |
HYPH |
denotes |
- |
T31789 |
1035-1036 |
CD |
denotes |
3 |
T31790 |
1037-1038 |
-LRB- |
denotes |
( |
T31791 |
1038-1040 |
NN |
denotes |
SC |
T31792 |
1040-1041 |
HYPH |
denotes |
- |
T31793 |
1041-1044 |
CD |
denotes |
878 |
T31794 |
1044-1045 |
-RRB- |
denotes |
) |
T31795 |
1045-1047 |
, |
denotes |
, |
T31796 |
1047-1053 |
NN |
denotes |
Cdkn1a |
T31797 |
1054-1055 |
-LRB- |
denotes |
( |
T31798 |
1055-1058 |
NN |
denotes |
p21 |
T31799 |
1058-1060 |
, |
denotes |
, |
T31800 |
1060-1062 |
NN |
denotes |
SC |
T31801 |
1062-1063 |
HYPH |
denotes |
- |
T31802 |
1063-1066 |
CD |
denotes |
471 |
T31803 |
1066-1067 |
-RRB- |
denotes |
) |
T31804 |
1067-1069 |
, |
denotes |
, |
T31805 |
1069-1075 |
NN |
denotes |
Cdkn1b |
T31806 |
1076-1077 |
-LRB- |
denotes |
( |
T31807 |
1077-1080 |
NN |
denotes |
p27 |
T31808 |
1080-1082 |
, |
denotes |
, |
T31809 |
1082-1084 |
NN |
denotes |
SC |
T31810 |
1084-1085 |
HYPH |
denotes |
- |
T31811 |
1085-1088 |
CD |
denotes |
528 |
T31812 |
1088-1089 |
-RRB- |
denotes |
) |
T31813 |
1089-1091 |
, |
denotes |
, |
T31814 |
1091-1097 |
NN |
denotes |
Pou4f2 |
T31815 |
1098-1099 |
-LRB- |
denotes |
( |
T31816 |
1099-1104 |
NN |
denotes |
Brn3b |
T31817 |
1104-1106 |
, |
denotes |
, |
T31818 |
1106-1108 |
NN |
denotes |
SC |
T31819 |
1108-1109 |
HYPH |
denotes |
- |
T31820 |
1109-1113 |
CD |
denotes |
6062 |
T31821 |
1113-1114 |
-RRB- |
denotes |
) |
T31822 |
1114-1116 |
, |
denotes |
, |
T31823 |
1116-1119 |
CC |
denotes |
and |
T31824 |
1120-1125 |
NN |
denotes |
Tfdp1 |
T31825 |
1126-1127 |
-LRB- |
denotes |
( |
T31826 |
1127-1130 |
NN |
denotes |
Dp1 |
T31827 |
1130-1132 |
, |
denotes |
, |
T31828 |
1132-1134 |
NN |
denotes |
SC |
T31829 |
1134-1135 |
HYPH |
denotes |
- |
T31830 |
1135-1138 |
CD |
denotes |
610 |
T31831 |
1138-1139 |
-RRB- |
denotes |
) |
T31832 |
1140-1144 |
IN |
denotes |
from |
T31833 |
1145-1150 |
NNP |
denotes |
Santa |
T31834 |
1156-1169 |
NNP |
denotes |
Biotechnology |
T31835 |
1151-1155 |
NNP |
denotes |
Cruz |
T31836 |
1170-1171 |
-LRB- |
denotes |
( |
T31837 |
1171-1190 |
NN |
denotes |
http://www.scbt.com |
T31838 |
1190-1191 |
-RRB- |
denotes |
) |
T31839 |
1191-1193 |
, |
denotes |
, |
T31840 |
1193-1196 |
NN |
denotes |
pRB |
T31841 |
1197-1198 |
-LRB- |
denotes |
( |
T31842 |
1198-1204 |
CD |
denotes |
554136 |
T31843 |
1204-1205 |
-RRB- |
denotes |
) |
T31844 |
1206-1210 |
IN |
denotes |
from |
T31845 |
1211-1213 |
NNP |
denotes |
BD |
T31846 |
1222-1232 |
NNP |
denotes |
Pharmingen |
T31847 |
1214-1221 |
NNP |
denotes |
Science |
T31848 |
1221-1222 |
HYPH |
denotes |
- |
T31849 |
1233-1234 |
-LRB- |
denotes |
( |
T31850 |
1234-1262 |
NN |
denotes |
http://www.bdbiosciences.com |
T31851 |
1262-1263 |
-RRB- |
denotes |
) |
T31852 |
1263-1265 |
, |
denotes |
, |
T31853 |
1265-1268 |
CC |
denotes |
and |
T31854 |
1269-1276 |
NN |
denotes |
Slc18a3 |
T31855 |
1277-1278 |
-LRB- |
denotes |
( |
T31856 |
1278-1283 |
NN |
denotes |
VAChT |
T31857 |
1283-1285 |
, |
denotes |
, |
T31858 |
1285-1290 |
NN |
denotes |
G448A |
T31859 |
1290-1291 |
-RRB- |
denotes |
) |
T31860 |
1292-1296 |
IN |
denotes |
from |
T31861 |
1297-1304 |
NNP |
denotes |
Promega |
T31862 |
1305-1306 |
-LRB- |
denotes |
( |
T31863 |
1306-1328 |
NN |
denotes |
http://www.promega.com |
T31864 |
1328-1329 |
-RRB- |
denotes |
) |
T31865 |
1329-1330 |
. |
denotes |
. |
R9426 |
T31588 |
T31589 |
compound |
Western,blots |
R9427 |
T31590 |
T31589 |
punct |
.,blots |
R9428 |
T31592 |
T31593 |
compound |
Mouse,retinas |
R9429 |
T31593 |
T31594 |
nsubjpass |
retinas,homogenized |
R9430 |
T31595 |
T31594 |
auxpass |
were,homogenized |
R9431 |
T31596 |
T31594 |
prep |
by,homogenized |
R9432 |
T31597 |
T31596 |
pcomp |
passing,by |
R9433 |
T31598 |
T31597 |
dobj |
them,passing |
R9434 |
T31599 |
T31597 |
prep |
through,passing |
R9435 |
T31600 |
T31601 |
det |
a,needle |
R9436 |
T31601 |
T31599 |
pobj |
needle,through |
R9437 |
T31602 |
T31603 |
nummod |
30,gauge |
R9438 |
T31603 |
T31601 |
nmod |
gauge,needle |
R9439 |
T31604 |
T31603 |
punct |
-,gauge |
R9440 |
T31605 |
T31601 |
nmod |
BD,needle |
R9441 |
T31606 |
T31605 |
nummod |
9,BD |
R9442 |
T31607 |
T31605 |
appos |
http://www.bd.com,BD |
R9443 |
T31608 |
T31601 |
punct |
),needle |
R9444 |
T31609 |
T31610 |
quantmod |
5,10 |
R9445 |
T31610 |
T31612 |
nummod |
10,times |
R9446 |
T31611 |
T31610 |
punct |
–,10 |
R9447 |
T31612 |
T31594 |
npadvmod |
times,homogenized |
R9448 |
T31613 |
T31594 |
prep |
in,homogenized |
R9449 |
T31614 |
T31615 |
nummod |
1,solution |
R9450 |
T31615 |
T31613 |
pobj |
solution,in |
R9451 |
T31616 |
T31614 |
punct |
×,1 |
R9452 |
T31617 |
T31615 |
compound |
PBS,solution |
R9453 |
T31618 |
T31594 |
punct |
.,homogenized |
R9454 |
T31620 |
T31621 |
amod |
Nuclear,proteins |
R9455 |
T31621 |
T31624 |
nsubjpass |
proteins,extracted |
R9456 |
T31622 |
T31620 |
cc |
and,Nuclear |
R9457 |
T31623 |
T31620 |
conj |
cytoplasmic,Nuclear |
R9458 |
T31625 |
T31624 |
auxpass |
were,extracted |
R9459 |
T31626 |
T31624 |
advcl |
using,extracted |
R9460 |
T31627 |
T31628 |
det |
the,Kit |
R9461 |
T31628 |
T31626 |
dobj |
Kit,using |
R9462 |
T31629 |
T31630 |
nmod |
NE,PER |
R9463 |
T31630 |
T31628 |
nmod |
PER,Kit |
R9464 |
T31631 |
T31630 |
punct |
-,PER |
R9465 |
T31632 |
T31628 |
amod |
Nuclear,Kit |
R9466 |
T31633 |
T31632 |
cc |
and,Nuclear |
R9467 |
T31634 |
T31632 |
conj |
Cytoplasmic,Nuclear |
R9468 |
T31635 |
T31628 |
compound |
Extraction,Kit |
R9469 |
T31636 |
T31628 |
punct |
(,Kit |
R9470 |
T31637 |
T31628 |
appos |
Product,Kit |
R9471 |
T31638 |
T31637 |
punct |
#,Product |
R9472 |
T31639 |
T31637 |
nummod |
78833,Product |
R9473 |
T31640 |
T31641 |
punct |
", ",Biotechnology |
R9474 |
T31641 |
T31628 |
parataxis |
Biotechnology,Kit |
R9475 |
T31642 |
T31641 |
compound |
Pierce,Biotechnology |
R9476 |
T31643 |
T31641 |
punct |
", ",Biotechnology |
R9477 |
T31644 |
T31641 |
npadvmod |
http://www.piercenet.com,Biotechnology |
R9478 |
T31645 |
T31641 |
punct |
),Biotechnology |
R9479 |
T31646 |
T31624 |
punct |
.,extracted |
R9480 |
T31648 |
T31649 |
nsubjpass |
Proteins,separated |
R9481 |
T31650 |
T31649 |
auxpass |
were,separated |
R9482 |
T31651 |
T31649 |
prep |
by,separated |
R9483 |
T31652 |
T31653 |
nummod |
10,% |
R9484 |
T31653 |
T31654 |
compound |
%,PAGE |
R9485 |
T31654 |
T31651 |
pobj |
PAGE,by |
R9486 |
T31655 |
T31654 |
compound |
SDS,PAGE |
R9487 |
T31656 |
T31654 |
punct |
-,PAGE |
R9488 |
T31657 |
T31649 |
cc |
and,separated |
R9489 |
T31658 |
T31649 |
conj |
transferred,separated |
R9490 |
T31659 |
T31658 |
prep |
to,transferred |
R9491 |
T31660 |
T31659 |
pobj |
nitrocellulose,to |
R9492 |
T31661 |
T31649 |
punct |
.,separated |
R9493 |
T31663 |
T31664 |
prep |
After,incubated |
R9494 |
T31665 |
T31663 |
pcomp |
blocking,After |
R9495 |
T31666 |
T31665 |
advmod |
overnight,blocking |
R9496 |
T31667 |
T31665 |
prep |
at,blocking |
R9497 |
T31668 |
T31669 |
nummod |
4,°C |
R9498 |
T31669 |
T31667 |
pobj |
°C,at |
R9499 |
T31670 |
T31665 |
prep |
in,blocking |
R9500 |
T31671 |
T31672 |
nummod |
5,% |
R9501 |
T31672 |
T31673 |
compound |
%,milk |
R9502 |
T31673 |
T31670 |
pobj |
milk,in |
R9503 |
T31674 |
T31673 |
compound |
skim,milk |
R9504 |
T31675 |
T31664 |
punct |
", ",incubated |
R9505 |
T31676 |
T31664 |
nsubjpass |
membranes,incubated |
R9506 |
T31677 |
T31664 |
auxpass |
were,incubated |
R9507 |
T31678 |
T31664 |
prep |
in,incubated |
R9508 |
T31679 |
T31680 |
det |
the,antibody |
R9509 |
T31680 |
T31678 |
pobj |
antibody,in |
R9510 |
T31681 |
T31680 |
amod |
primary,antibody |
R9511 |
T31682 |
T31664 |
prep |
for,incubated |
R9512 |
T31683 |
T31684 |
nummod |
2,h |
R9513 |
T31684 |
T31682 |
pobj |
h,for |
R9514 |
T31685 |
T31664 |
prep |
at,incubated |
R9515 |
T31686 |
T31687 |
compound |
room,temperature |
R9516 |
T31687 |
T31685 |
pobj |
temperature,at |
R9517 |
T31688 |
T31664 |
punct |
.,incubated |
R9518 |
T31690 |
T31691 |
prep |
After,incubated |
R9519 |
T31692 |
T31693 |
nummod |
three,washes |
R9520 |
T31693 |
T31690 |
pobj |
washes,After |
R9521 |
T31694 |
T31695 |
nummod |
10,min |
R9522 |
T31695 |
T31693 |
compound |
min,washes |
R9523 |
T31696 |
T31695 |
punct |
-,min |
R9524 |
T31697 |
T31693 |
prep |
in,washes |
R9525 |
T31698 |
T31697 |
pobj |
TPBS,in |
R9526 |
T31699 |
T31698 |
punct |
(,TPBS |
R9527 |
T31700 |
T31701 |
nummod |
100,mM |
R9528 |
T31701 |
T31702 |
compound |
mM,Na2HPO4 |
R9529 |
T31702 |
T31698 |
appos |
Na2HPO4,TPBS |
R9530 |
T31703 |
T31702 |
punct |
", ",Na2HPO4 |
R9531 |
T31704 |
T31705 |
nummod |
100,mM |
R9532 |
T31705 |
T31706 |
compound |
mM,NaH2PO4 |
R9533 |
T31706 |
T31702 |
appos |
NaH2PO4,Na2HPO4 |
R9534 |
T31707 |
T31702 |
punct |
", ",Na2HPO4 |
R9535 |
T31708 |
T31709 |
nummod |
0.5,N |
R9536 |
T31709 |
T31710 |
compound |
N,NaCl |
R9537 |
T31710 |
T31702 |
appos |
NaCl,Na2HPO4 |
R9538 |
T31711 |
T31702 |
punct |
", ",Na2HPO4 |
R9539 |
T31712 |
T31713 |
nummod |
0.1,% |
R9540 |
T31713 |
T31714 |
compound |
%,Tween |
R9541 |
T31714 |
T31702 |
appos |
Tween,Na2HPO4 |
R9542 |
T31715 |
T31714 |
punct |
-,Tween |
R9543 |
T31716 |
T31714 |
nummod |
20,Tween |
R9544 |
T31717 |
T31691 |
punct |
),incubated |
R9545 |
T31718 |
T31691 |
punct |
", ",incubated |
R9546 |
T31719 |
T31691 |
nsubjpass |
membranes,incubated |
R9547 |
T31720 |
T31691 |
auxpass |
were,incubated |
R9548 |
T31721 |
T31691 |
prep |
for,incubated |
R9549 |
T31722 |
T31723 |
nummod |
30,min |
R9550 |
T31723 |
T31721 |
pobj |
min,for |
R9551 |
T31724 |
T31691 |
prep |
at,incubated |
R9552 |
T31725 |
T31726 |
compound |
room,temperature |
R9553 |
T31726 |
T31724 |
pobj |
temperature,at |
R9554 |
T31727 |
T31691 |
prep |
in,incubated |
R9555 |
T31728 |
T31729 |
det |
the,antibody |
R9556 |
T31729 |
T31727 |
pobj |
antibody,in |
R9557 |
T31730 |
T31729 |
amod |
secondary,antibody |
R9558 |
T31731 |
T31732 |
compound |
horseradish,peroxidase |
R9559 |
T31732 |
T31733 |
npadvmod |
peroxidase,conjugated |
R9560 |
T31733 |
T31729 |
amod |
conjugated,antibody |
R9561 |
T31734 |
T31733 |
punct |
-,conjugated |
R9562 |
T31735 |
T31736 |
punct |
(,Laboratories |
R9563 |
T31736 |
T31729 |
parataxis |
Laboratories,antibody |
R9564 |
T31737 |
T31736 |
compound |
Jackson,Laboratories |
R9565 |
T31738 |
T31736 |
compound |
ImmunoResearch,Laboratories |
R9566 |
T31739 |
T31736 |
punct |
", ",Laboratories |
R9567 |
T31740 |
T31736 |
npadvmod |
http://www.jacksonimmuno.com,Laboratories |
R9568 |
T31741 |
T31736 |
punct |
),Laboratories |
R9569 |
T31742 |
T31691 |
punct |
.,incubated |
R9570 |
T31744 |
T31745 |
nsubjpass |
Blots,developed |
R9571 |
T31746 |
T31745 |
auxpass |
were,developed |
R9572 |
T31747 |
T31745 |
advcl |
using,developed |
R9573 |
T31748 |
T31749 |
det |
the,system |
R9574 |
T31749 |
T31747 |
dobj |
system,using |
R9575 |
T31750 |
T31751 |
nmod |
ECL,Plus |
R9576 |
T31751 |
T31749 |
nmod |
Plus,system |
R9577 |
T31752 |
T31751 |
punct |
-,Plus |
R9578 |
T31753 |
T31749 |
amod |
chemiluminescent,system |
R9579 |
T31754 |
T31749 |
compound |
detection,system |
R9580 |
T31755 |
T31756 |
punct |
(,Biotech |
R9581 |
T31756 |
T31749 |
parataxis |
Biotech,system |
R9582 |
T31757 |
T31756 |
compound |
Amersham,Biotech |
R9583 |
T31758 |
T31756 |
compound |
Pharmacia,Biotech |
R9584 |
T31759 |
T31756 |
punct |
", ",Biotech |
R9585 |
T31760 |
T31756 |
npadvmod |
http://www.pharmacia.ca,Biotech |
R9586 |
T31761 |
T31756 |
punct |
),Biotech |
R9587 |
T31762 |
T31747 |
punct |
", ",using |
R9588 |
T31763 |
T31747 |
prep |
according,using |
R9589 |
T31764 |
T31763 |
prep |
to,according |
R9590 |
T31765 |
T31766 |
det |
the,manufacturer |
R9591 |
T31766 |
T31767 |
poss |
manufacturer,instructions |
R9592 |
T31767 |
T31764 |
pobj |
instructions,to |
R9593 |
T31768 |
T31766 |
case |
's,manufacturer |
R9594 |
T31769 |
T31745 |
punct |
.,developed |
R9595 |
T31771 |
T31772 |
det |
The,antibodies |
R9596 |
T31772 |
T31775 |
nsubjpass |
antibodies,used |
R9597 |
T31773 |
T31772 |
amod |
following,antibodies |
R9598 |
T31774 |
T31772 |
amod |
primary,antibodies |
R9599 |
T31776 |
T31775 |
auxpass |
were,used |
R9600 |
T31777 |
T31775 |
punct |
: ,used |
R9601 |
T31778 |
T31775 |
dobj |
E2f,used |
R9602 |
T31779 |
T31778 |
punct |
-,E2f |
R9603 |
T31780 |
T31778 |
nummod |
1,E2f |
R9604 |
T31781 |
T31778 |
punct |
(,E2f |
R9605 |
T31782 |
T31778 |
appos |
SC,E2f |
R9606 |
T31783 |
T31782 |
punct |
-,SC |
R9607 |
T31784 |
T31782 |
nummod |
193,SC |
R9608 |
T31785 |
T31778 |
punct |
),E2f |
R9609 |
T31786 |
T31778 |
punct |
", ",E2f |
R9610 |
T31787 |
T31778 |
conj |
E2f,E2f |
R9611 |
T31788 |
T31787 |
punct |
-,E2f |
R9612 |
T31789 |
T31787 |
nummod |
3,E2f |
R9613 |
T31790 |
T31787 |
punct |
(,E2f |
R9614 |
T31791 |
T31787 |
appos |
SC,E2f |
R9615 |
T31792 |
T31791 |
punct |
-,SC |
R9616 |
T31793 |
T31791 |
nummod |
878,SC |
R9617 |
T31794 |
T31787 |
punct |
),E2f |
R9618 |
T31795 |
T31787 |
punct |
", ",E2f |
R9619 |
T31796 |
T31787 |
conj |
Cdkn1a,E2f |
R9620 |
T31797 |
T31796 |
punct |
(,Cdkn1a |
R9621 |
T31798 |
T31796 |
appos |
p21,Cdkn1a |
R9622 |
T31799 |
T31796 |
punct |
", ",Cdkn1a |
R9623 |
T31800 |
T31796 |
appos |
SC,Cdkn1a |
R9624 |
T31801 |
T31800 |
punct |
-,SC |
R9625 |
T31802 |
T31800 |
nummod |
471,SC |
R9626 |
T31803 |
T31796 |
punct |
),Cdkn1a |
R9627 |
T31804 |
T31796 |
punct |
", ",Cdkn1a |
R9628 |
T31805 |
T31796 |
conj |
Cdkn1b,Cdkn1a |
R9629 |
T31806 |
T31805 |
punct |
(,Cdkn1b |
R9630 |
T31807 |
T31805 |
appos |
p27,Cdkn1b |
R9631 |
T31808 |
T31805 |
punct |
", ",Cdkn1b |
R9632 |
T31809 |
T31805 |
appos |
SC,Cdkn1b |
R9633 |
T31810 |
T31809 |
punct |
-,SC |
R9634 |
T31811 |
T31809 |
nummod |
528,SC |
R9635 |
T31812 |
T31805 |
punct |
),Cdkn1b |
R9636 |
T31813 |
T31805 |
punct |
", ",Cdkn1b |
R9637 |
T31814 |
T31805 |
conj |
Pou4f2,Cdkn1b |
R9638 |
T31815 |
T31814 |
punct |
(,Pou4f2 |
R9639 |
T31816 |
T31814 |
appos |
Brn3b,Pou4f2 |
R9640 |
T31817 |
T31814 |
punct |
", ",Pou4f2 |
R9641 |
T31818 |
T31814 |
appos |
SC,Pou4f2 |
R9642 |
T31819 |
T31818 |
punct |
-,SC |
R9643 |
T31820 |
T31818 |
nummod |
6062,SC |
R9644 |
T31821 |
T31814 |
punct |
),Pou4f2 |
R9645 |
T31822 |
T31814 |
punct |
", ",Pou4f2 |
R9646 |
T31823 |
T31814 |
cc |
and,Pou4f2 |
R9647 |
T31824 |
T31814 |
conj |
Tfdp1,Pou4f2 |
R9648 |
T31825 |
T31824 |
punct |
(,Tfdp1 |
R9649 |
T31826 |
T31824 |
appos |
Dp1,Tfdp1 |
R9650 |
T31827 |
T31824 |
punct |
", ",Tfdp1 |
R9651 |
T31828 |
T31824 |
appos |
SC,Tfdp1 |
R9652 |
T31829 |
T31828 |
punct |
-,SC |
R9653 |
T31830 |
T31828 |
nummod |
610,SC |
R9654 |
T31831 |
T31778 |
punct |
),E2f |
R9655 |
T31832 |
T31778 |
prep |
from,E2f |
R9656 |
T31833 |
T31834 |
compound |
Santa,Biotechnology |
R9657 |
T31834 |
T31832 |
pobj |
Biotechnology,from |
R9658 |
T31835 |
T31834 |
compound |
Cruz,Biotechnology |
R9659 |
T31836 |
T31834 |
punct |
(,Biotechnology |
R9660 |
T31837 |
T31834 |
npadvmod |
http://www.scbt.com,Biotechnology |
R9661 |
T31838 |
T31778 |
punct |
),E2f |
R9662 |
T31839 |
T31778 |
punct |
", ",E2f |
R9663 |
T31840 |
T31778 |
conj |
pRB,E2f |
R9664 |
T31841 |
T31840 |
punct |
(,pRB |
R9665 |
T31842 |
T31840 |
appos |
554136,pRB |
R9666 |
T31843 |
T31840 |
punct |
),pRB |
R9667 |
T31844 |
T31840 |
prep |
from,pRB |
R9668 |
T31845 |
T31846 |
compound |
BD,Pharmingen |
R9669 |
T31846 |
T31844 |
pobj |
Pharmingen,from |
R9670 |
T31847 |
T31846 |
compound |
Science,Pharmingen |
R9671 |
T31848 |
T31846 |
punct |
-,Pharmingen |
R9672 |
T31849 |
T31846 |
punct |
(,Pharmingen |
R9673 |
T31850 |
T31846 |
npadvmod |
http://www.bdbiosciences.com,Pharmingen |
R9674 |
T31851 |
T31840 |
punct |
),pRB |
R9675 |
T31852 |
T31840 |
punct |
", ",pRB |
R9676 |
T31853 |
T31840 |
cc |
and,pRB |
R9677 |
T31854 |
T31840 |
conj |
Slc18a3,pRB |
R9678 |
T31855 |
T31854 |
punct |
(,Slc18a3 |
R9679 |
T31856 |
T31854 |
appos |
VAChT,Slc18a3 |
R9680 |
T31857 |
T31854 |
punct |
", ",Slc18a3 |
R9681 |
T31858 |
T31854 |
appos |
G448A,Slc18a3 |
R9682 |
T31859 |
T31854 |
punct |
),Slc18a3 |
R9683 |
T31860 |
T31854 |
prep |
from,Slc18a3 |
R9684 |
T31861 |
T31860 |
pobj |
Promega,from |
R9685 |
T31862 |
T31861 |
punct |
(,Promega |
R9686 |
T31863 |
T31861 |
npadvmod |
http://www.promega.com,Promega |
R9687 |
T31864 |
T31775 |
punct |
),used |
R9688 |
T31865 |
T31775 |
punct |
.,used |