PMC:1914394 / 57889-58021 JSONTXT

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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T30734","span":{"begin":14,"end":18},"obj":"SO_EXT:cDNA"},{"id":"T30735","span":{"begin":60,"end":63},"obj":"CHEBI_SO_EXT:RNA"}],"text":" First-strand cDNA was synthesized from 0.2–0.5 μg of total RNA using the SuperScript II first-strand synthesis system (Invitrogen)."}