PMC:1914094 / 35499-35892 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1914094","sourcedb":"PMC","sourceid":"1914094","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1914094","text":"CD30L binding assay\nA total of 2.5 × 105 cells were incubated with 5 μM Trx1 (SBP-CCCCC) and 200 μM DTT for 30 min at 37°C, washed three times and incubated with 250 ng/ml recombinant CD30L-His10 (R\u0026D Systems) for 10 min at RT. After washing, cells were stained with anti-polyHis monoclonal antibody (Sigma) followed by incubation with R-PE-conjugated goat F(ab')2 anti-mouse Ig's (Biosource).","divisions":[{"label":"Title","span":{"begin":0,"end":19}}],"tracks":[]}