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PMC:1892049 / 21271-21945 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T10098 147-153 UBERON:0000955 denotes brains
T10099 163-168 GO:0007567 denotes natal
T10100 210-216 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T10101 234-238 CHEBI:9754 denotes Tris
T10102 239-242 CHEBI:17883 denotes HCl
T10103 251-255 CHEBI:26710 denotes NaCl
T10104 262-266 CHEBI_EXT:EDTA denotes EDTA
T10105 271-283 CHEBI:9750 denotes Triton X-100
T10106 288-307 CHEBI:9177 denotes sodium deoxycholate
T10107 314-317 CHEBI:8984 denotes SDS
T10108 325-333 CHEBI:60004 denotes cocktail
T10109 337-356 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T10110 442-450 MOP:0000569 denotes reducing
T10111 458-464 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T10112 550-553 CHEBI:8984 denotes SDS
T10113 583-593 GO:0042571 denotes antibodies
T10114 597-602 PR_EXT:000009158 denotes Itpr1
T10115 617-621 PR_EXT:000003676 denotes Actb

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T10081 147-153 UBERON:0000955 denotes brains
T10082 163-168 GO:0007567 denotes natal
T10083 234-238 CHEBI:9754 denotes Tris
T10084 239-242 CHEBI:17883 denotes HCl
T10085 251-255 CHEBI:26710 denotes NaCl
T10086 271-283 CHEBI:9750 denotes Triton X-100
T10087 288-307 CHEBI:9177 denotes sodium deoxycholate
T10088 314-317 CHEBI:8984 denotes SDS
T10089 325-333 CHEBI:60004 denotes cocktail
T10090 442-450 MOP:0000569 denotes reducing
T10091 550-553 CHEBI:8984 denotes SDS
T10092 583-593 GO:0042571 denotes antibodies
T10093 597-602 PR:000009158 denotes Itpr1
T10094 617-621 PR:000003676 denotes Actb

craft-sa-dev

Id Subject Object Predicate Lexical cue
T10124 0-2 PRP denotes We
T10125 3-12 VBD denotes performed
T10126 13-20 NNP denotes Western
T10127 21-25 NN denotes blot
T10128 26-34 NNS denotes analyses
T10129 35-40 VBG denotes using
T10130 41-49 JJ denotes standard
T10131 50-60 NNS denotes techniques
T10132 61-65 IN denotes with
T10133 66-69 NN denotes ECL
T10134 80-84 NNS denotes kits
T10135 70-79 NN denotes detection
T10136 85-86 -LRB- denotes (
T10137 86-94 NNP denotes Amersham
T10138 94-96 , denotes ,
T10139 96-119 NN denotes http://www.amersham.com
T10140 119-120 -RRB- denotes )
T10141 120-121 . denotes .
T10142 121-387 sentence denotes Briefly, dissected whole brains from postnatal day 21 littermates were homogenized in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and a cocktail of protease inhibitors (Roche, http://www.roche.com).
T10143 122-129 RB denotes Briefly
T10144 193-204 VBN denotes homogenized
T10145 129-131 , denotes ,
T10146 131-140 VBN denotes dissected
T10147 147-153 NNS denotes brains
T10148 141-146 JJ denotes whole
T10149 154-158 IN denotes from
T10150 159-168 JJ denotes postnatal
T10151 169-172 NN denotes day
T10152 176-187 NNS denotes littermates
T10153 173-175 CD denotes 21
T10154 188-192 VBD denotes were
T10155 205-207 IN denotes in
T10156 208-209 DT denotes a
T10157 210-216 NN denotes buffer
T10158 217-227 VBG denotes containing
T10159 228-230 CD denotes 50
T10160 231-233 NN denotes mM
T10161 239-242 NN denotes HCl
T10162 234-238 NN denotes Tris
T10163 238-239 HYPH denotes -
T10164 242-244 , denotes ,
T10165 244-247 CD denotes 150
T10166 248-250 NN denotes mM
T10167 251-255 NN denotes NaCl
T10168 255-257 , denotes ,
T10169 257-258 CD denotes 1
T10170 259-261 NN denotes mM
T10171 262-266 NN denotes EDTA
T10172 266-268 , denotes ,
T10173 268-269 CD denotes 1
T10174 269-270 NN denotes %
T10175 278-279 NN denotes X
T10176 271-277 NN denotes Triton
T10177 279-280 HYPH denotes -
T10178 280-283 CD denotes 100
T10179 283-285 , denotes ,
T10180 285-286 CD denotes 1
T10181 286-287 NN denotes %
T10182 295-307 NN denotes deoxycholate
T10183 288-294 NN denotes sodium
T10184 307-309 , denotes ,
T10185 309-312 CD denotes 0.1
T10186 312-313 NN denotes %
T10187 314-317 NN denotes SDS
T10188 317-319 , denotes ,
T10189 319-322 CC denotes and
T10190 323-324 DT denotes a
T10191 325-333 NN denotes cocktail
T10192 334-336 IN denotes of
T10193 337-345 NN denotes protease
T10194 346-356 NNS denotes inhibitors
T10195 357-358 -LRB- denotes (
T10196 358-363 NNP denotes Roche
T10197 363-365 , denotes ,
T10198 365-385 NN denotes http://www.roche.com
T10199 385-386 -RRB- denotes )
T10200 386-387 . denotes .
T10201 387-578 sentence denotes Homogenates were diluted appropriately, mixed with 4× reducing sample buffer, and loaded onto 4%–12% precast gradient gels (Novex, http://www.invitrogen.com) for SDS-PAGE and immunoblotting.
T10202 388-399 NNS denotes Homogenates
T10203 405-412 VBN denotes diluted
T10204 400-404 VBD denotes were
T10205 413-426 RB denotes appropriately
T10206 426-428 , denotes ,
T10207 428-433 VBN denotes mixed
T10208 434-438 IN denotes with
T10209 439-440 CD denotes 4
T10210 458-464 NN denotes buffer
T10211 440-441 SYM denotes ×
T10212 442-450 VBG denotes reducing
T10213 451-457 NN denotes sample
T10214 464-466 , denotes ,
T10215 466-469 CC denotes and
T10216 470-476 VBN denotes loaded
T10217 477-481 IN denotes onto
T10218 482-483 CD denotes 4
T10219 483-484 NN denotes %
T10220 506-510 NNS denotes gels
T10221 484-485 SYM denotes –
T10222 487-488 NN denotes %
T10223 485-487 CD denotes 12
T10224 489-496 NN denotes precast
T10225 497-505 NN denotes gradient
T10226 511-512 -LRB- denotes (
T10227 512-517 NNP denotes Novex
T10228 517-519 , denotes ,
T10229 519-544 NN denotes http://www.invitrogen.com
T10230 544-545 -RRB- denotes )
T10231 546-549 IN denotes for
T10232 550-553 NN denotes SDS
T10233 554-558 NN denotes PAGE
T10234 553-554 HYPH denotes -
T10235 559-562 CC denotes and
T10236 563-577 NN denotes immunoblotting
T10237 577-578 . denotes .
T10238 578-674 sentence denotes The antibodies to Itpr1 (1:2,000) and Actb (1:5,000) were used as recommended by manufacturers.
T10239 579-582 DT denotes The
T10240 583-593 NNS denotes antibodies
T10241 637-641 VBN denotes used
T10242 594-596 IN denotes to
T10243 597-602 NN denotes Itpr1
T10244 603-604 -LRB- denotes (
T10245 604-605 CD denotes 1
T10246 605-606 SYM denotes :
T10247 606-611 CD denotes 2,000
T10248 611-612 -RRB- denotes )
T10249 613-616 CC denotes and
T10250 617-621 NN denotes Actb
T10251 622-623 -LRB- denotes (
T10252 623-624 CD denotes 1
T10253 624-625 SYM denotes :
T10254 625-630 CD denotes 5,000
T10255 630-631 -RRB- denotes )
T10256 632-636 VBD denotes were
T10257 642-644 IN denotes as
T10258 645-656 VBN denotes recommended
T10259 657-659 IN denotes by
T10260 660-673 NNS denotes manufacturers
T10261 673-674 . denotes .
R2905 T10124 T10125 nsubj We,performed
R2906 T10126 T10127 compound Western,blot
R2907 T10127 T10128 compound blot,analyses
R2908 T10128 T10125 dobj analyses,performed
R2909 T10129 T10125 advcl using,performed
R2910 T10130 T10131 amod standard,techniques
R2911 T10131 T10129 dobj techniques,using
R2912 T10132 T10125 prep with,performed
R2913 T10133 T10134 compound ECL,kits
R2914 T10134 T10132 pobj kits,with
R2915 T10135 T10134 compound detection,kits
R2916 T10136 T10137 punct (,Amersham
R2917 T10137 T10134 parataxis Amersham,kits
R2918 T10138 T10137 punct ", ",Amersham
R2919 T10139 T10137 npadvmod http://www.amersham.com,Amersham
R2920 T10140 T10137 punct ),Amersham
R2921 T10141 T10125 punct .,performed
R2922 T10143 T10144 advmod Briefly,homogenized
R2923 T10145 T10144 punct ", ",homogenized
R2924 T10146 T10147 amod dissected,brains
R2925 T10147 T10144 nsubjpass brains,homogenized
R2926 T10148 T10147 amod whole,brains
R2927 T10149 T10147 prep from,brains
R2928 T10150 T10151 amod postnatal,day
R2929 T10151 T10152 nmod day,littermates
R2930 T10152 T10149 pobj littermates,from
R2931 T10153 T10151 nummod 21,day
R2932 T10154 T10144 auxpass were,homogenized
R2933 T10155 T10144 prep in,homogenized
R2934 T10156 T10157 det a,buffer
R2935 T10157 T10155 pobj buffer,in
R2936 T10158 T10157 acl containing,buffer
R2937 T10159 T10160 nummod 50,mM
R2938 T10160 T10161 compound mM,HCl
R2939 T10161 T10158 dobj HCl,containing
R2940 T10162 T10161 compound Tris,HCl
R2941 T10163 T10161 punct -,HCl
R2942 T10164 T10161 punct ", ",HCl
R2943 T10165 T10166 nummod 150,mM
R2944 T10166 T10167 compound mM,NaCl
R2945 T10167 T10161 conj NaCl,HCl
R2946 T10168 T10167 punct ", ",NaCl
R2947 T10169 T10170 nummod 1,mM
R2948 T10170 T10171 compound mM,EDTA
R2949 T10171 T10167 conj EDTA,NaCl
R2950 T10172 T10171 punct ", ",EDTA
R2951 T10173 T10174 nummod 1,%
R2952 T10174 T10175 compound %,X
R2953 T10175 T10171 conj X,EDTA
R2954 T10176 T10175 compound Triton,X
R2955 T10177 T10175 punct -,X
R2956 T10178 T10175 nummod 100,X
R2957 T10179 T10175 punct ", ",X
R2958 T10180 T10181 nummod 1,%
R2959 T10181 T10182 compound %,deoxycholate
R2960 T10182 T10175 conj deoxycholate,X
R2961 T10183 T10182 compound sodium,deoxycholate
R2962 T10184 T10182 punct ", ",deoxycholate
R2963 T10185 T10186 nummod 0.1,%
R2964 T10186 T10187 compound %,SDS
R2965 T10187 T10182 conj SDS,deoxycholate
R2966 T10188 T10187 punct ", ",SDS
R2967 T10189 T10187 cc and,SDS
R2968 T10190 T10191 det a,cocktail
R2969 T10191 T10187 conj cocktail,SDS
R2970 T10192 T10191 prep of,cocktail
R2971 T10193 T10194 compound protease,inhibitors
R2972 T10194 T10192 pobj inhibitors,of
R2973 T10195 T10196 punct (,Roche
R2974 T10196 T10191 parataxis Roche,cocktail
R2975 T10197 T10196 punct ", ",Roche
R2976 T10198 T10196 npadvmod http://www.roche.com,Roche
R2977 T10199 T10196 punct ),Roche
R2978 T10200 T10144 punct .,homogenized
R2979 T10202 T10203 nsubjpass Homogenates,diluted
R2980 T10204 T10203 auxpass were,diluted
R2981 T10205 T10203 advmod appropriately,diluted
R2982 T10206 T10203 punct ", ",diluted
R2983 T10207 T10203 conj mixed,diluted
R2984 T10208 T10207 prep with,mixed
R2985 T10209 T10210 nummod 4,buffer
R2986 T10210 T10208 pobj buffer,with
R2987 T10211 T10209 punct ×,4
R2988 T10212 T10210 amod reducing,buffer
R2989 T10213 T10210 compound sample,buffer
R2990 T10214 T10207 punct ", ",mixed
R2991 T10215 T10207 cc and,mixed
R2992 T10216 T10207 conj loaded,mixed
R2993 T10217 T10216 prep onto,loaded
R2994 T10218 T10219 nummod 4,%
R2995 T10219 T10220 nmod %,gels
R2996 T10220 T10217 pobj gels,onto
R2997 T10221 T10222 punct –,%
R2998 T10222 T10219 prep %,%
R2999 T10223 T10222 nummod 12,%
R3000 T10224 T10220 compound precast,gels
R3001 T10225 T10220 compound gradient,gels
R3002 T10226 T10227 punct (,Novex
R3003 T10227 T10220 parataxis Novex,gels
R3004 T10228 T10227 punct ", ",Novex
R3005 T10229 T10227 npadvmod http://www.invitrogen.com,Novex
R3006 T10230 T10227 punct ),Novex
R3007 T10231 T10216 prep for,loaded
R3008 T10232 T10233 compound SDS,PAGE
R3009 T10233 T10231 pobj PAGE,for
R3010 T10234 T10233 punct -,PAGE
R3011 T10235 T10233 cc and,PAGE
R3012 T10236 T10233 conj immunoblotting,PAGE
R3013 T10237 T10203 punct .,diluted
R3014 T10239 T10240 det The,antibodies
R3015 T10240 T10241 nsubjpass antibodies,used
R3016 T10242 T10240 prep to,antibodies
R3017 T10243 T10242 pobj Itpr1,to
R3018 T10244 T10245 punct (,1
R3019 T10245 T10243 parataxis 1,Itpr1
R3020 T10246 T10247 punct :,"2,000"
R3021 T10247 T10245 prep "2,000",1
R3022 T10248 T10245 punct ),1
R3023 T10249 T10243 cc and,Itpr1
R3024 T10250 T10243 conj Actb,Itpr1
R3025 T10251 T10252 punct (,1
R3026 T10252 T10250 parataxis 1,Actb
R3027 T10253 T10254 punct :,"5,000"
R3028 T10254 T10252 prep "5,000",1
R3029 T10255 T10252 punct ),1
R3030 T10256 T10241 auxpass were,used
R3031 T10257 T10258 mark as,recommended
R3032 T10258 T10241 advcl recommended,used
R3033 T10259 T10258 agent by,recommended
R3034 T10260 T10259 pobj manufacturers,by
R3035 T10261 T10241 punct .,used