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PMC:1892049 / 19166-28690 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T8952 23-29 SO_EXT:0001026 denotes Genome
T8953 46-50 NCBITaxon:10088 denotes mice
T8954 75-78 CHEBI_SO_EXT:DNA denotes DNA
T8955 115-121 SO_EXT:0001026 denotes genome
T8956 176-180 SO_EXT:0000694 denotes SNPs
T8957 311-315 NCBITaxon:10088 denotes mice
T8958 336-340 NCBITaxon:10088 denotes mice
T8959 342-350 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T8960 376-379 CHEBI_EXT:37958 denotes dye
T8961 525-535 GO_SO_EXT:chromosome denotes Chromosome
T8962 599-605 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T8963 764-768 NCBITaxon:10088 denotes mice
T8964 797-801 NCBITaxon:10088 denotes mice
T8965 851-859 SO:0000357 denotes flanking
T8966 860-872 SO_EXT:sequence_recombinant_entity denotes recombinants
T8967 903-904 CHEBI_SO_EXT:base denotes b
T8968 914-921 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T8969 986-991 SO_EXT:0000704 denotes genes
T8970 996-1017 SO_EXT:0002138 denotes predicted transcripts
T9560 1053-1060 SO_EXT:0000704 denotes genetic
T9561 1071-1075 NCBITaxon:10088 denotes mice
T9562 1117-1121 NCBITaxon:10088 denotes mice
T9563 1206-1211 PR_EXT:000009158 denotes Itpr1
T9564 1219-1224 NCBITaxon:10088 denotes mouse
T9565 1267-1275 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T9566 1276-1284 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T9567 1288-1293 SO_EXT:0000147 denotes exons
T9568 1307-1312 PR_EXT:000009158 denotes Itpr1
T9569 1314-1320 SO_EXT:0000112 denotes Primer
T9570 1365-1377 SO_EXT:0000195 denotes coding exons
T9571 1394-1396 SO_EXT:0000028 denotes bp
T9572 1405-1413 _FRAGMENT denotes flanking
T9573 1423-1431 SO_EXT:0000239 denotes sequence
T9574 1414-1422 SO_EXT:0000188 denotes intronic
T9575 1435-1440 PR_EXT:000009158 denotes Itpr1
T9576 1468-1472 SO_EXT:0000147 denotes exon
T9577 1493-1496 CHEBI_SO_EXT:DNA denotes DNA
T9578 1515-1519 NCBITaxon:10088 denotes mice
T9579 1538-1543 PR_EXT:000009158 denotes Itpr1
T9580 1551-1559 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T9581 1604-1608 NCBITaxon:10088 denotes mice
T9582 1622-1630 GO_EXT:breeding denotes Breeding
T9583 1670-1676 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T9584 1677-1681 NCBITaxon:10088 denotes mice
T9585 1711-1719 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T9586 1721-1726 PR_EXT:000009158 denotes Itpr1
T9587 1726-1728 SO_EXT:wild_type_entity_or_quality denotes wt
T9588 1740-1744 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T9589 1745-1750 NCBITaxon:10088 denotes mouse
T9590 1772-1777 PR_EXT:000009158 denotes Itpr1
T9591 1781-1789 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T9592 1791-1796 PR_EXT:000009158 denotes Itpr1
T9593 1796-1798 SO_EXT:wild_type_entity_or_quality denotes wt
T9594 1837-1841 NCBITaxon:10088 denotes mice
T9595 1872-1877 PR_EXT:000009158 denotes Itpr1
T9596 1885-1889 NCBITaxon_UBERON_EXT:pup denotes pups
T9597 1935-1941 GO:0007618 denotes mating
T9598 1972-1978 GO:0007618 denotes mating
T9599 2032-2037 PR_EXT:000009158 denotes Itpr1
T9600 2049-2054 PR_EXT:000009158 denotes Itpr1
T9601 2063-2067 NCBITaxon:10088 denotes mice
T10095 2082-2087 PR_EXT:000009158 denotes Itpr1
T10096 2088-2095 CHEBI_PR_EXT:protein denotes protein
T10097 2099-2103 NCBITaxon:10088 denotes mice
T10098 2252-2258 UBERON:0000955 denotes brains
T10099 2268-2273 GO:0007567 denotes natal
T10100 2315-2321 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T10101 2339-2343 CHEBI:9754 denotes Tris
T10102 2344-2347 CHEBI:17883 denotes HCl
T10103 2356-2360 CHEBI:26710 denotes NaCl
T10104 2367-2371 CHEBI_EXT:EDTA denotes EDTA
T10105 2376-2388 CHEBI:9750 denotes Triton X-100
T10106 2393-2412 CHEBI:9177 denotes sodium deoxycholate
T10107 2419-2422 CHEBI:8984 denotes SDS
T10108 2430-2438 CHEBI:60004 denotes cocktail
T10109 2442-2461 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T10110 2547-2555 MOP:0000569 denotes reducing
T10111 2563-2569 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T10112 2655-2658 CHEBI:8984 denotes SDS
T10113 2688-2698 GO:0042571 denotes antibodies
T10114 2702-2707 PR_EXT:000009158 denotes Itpr1
T10115 2722-2726 PR_EXT:000003676 denotes Actb
T10896 2803-2809 UBERON:0000955 denotes Brains
T10897 2838-2842 NCBITaxon:10088 denotes mice
T10898 2861-2877 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T10899 2923-2931 CHEBI_EXT:50913 denotes fixative
T10900 2933-2939 UBERON:0000955 denotes Brains
T10901 2957-2964 CHEBI:5291 denotes gelatin
T10902 3109-3118 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10903 3149-3155 UBERON:0000955 denotes brains
T10904 3180-3185 UBERON:0000955 denotes Brain
T10905 3263-3271 CHEBI:75958 denotes solution
T10906 3306-3310 NCBITaxon:9925 denotes goat
T10907 3311-3316 UBERON:0001977 denotes serum
T10908 3326-3338 CHEBI:9750 denotes Triton X-100
T10909 3402-3412 GO:0042571 denotes antibodies
T10910 3443-3448 PR_EXT:000009158 denotes Itpr1
T10911 3449-3457 GO:0042571 denotes antibody
T10912 3537-3542 PR_EXT:000004967 denotes Calb1
T10913 3543-3551 GO:0042571 denotes antibody
T10914 3617-3624 GO_EXT:biological_mover_or_transporter denotes carrier
T10915 3625-3633 CHEBI:75958 denotes solution
T10916 3746-3756 GO:0042571 denotes antibodies
T10917 3758-3773 CHEBI:52673 denotes Alexa Fluor 555
T10918 3774-3778 NCBITaxon:9925 denotes goat
T10919 3784-3790 NCBITaxon:9986 denotes rabbit
T10920 3791-3794 GO:0071735 denotes IgG
T10921 3799-3814 CHEBI:52661 denotes Alexa Fluor 488
T10922 3815-3819 NCBITaxon:9925 denotes goat
T10923 3825-3830 NCBITaxon:10088 denotes mouse
T10924 3831-3834 GO:0071735 denotes IgG
T10925 3959-3967 CHEBI_CHMO_EXT:buffer_process denotes buffered
T10926 4272-4281 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10927 4312-4318 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T10928 4319-4325 UBERON:0000955 denotes brains
T10929 4346-4351 PR_EXT:000009158 denotes Itpr1
T10930 4352-4362 GO:0042571 denotes antibodies
T10931 4414-4422 GO:0042571 denotes Antibody
T10932 4485-4492 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T10933 4494-4500 UBERON:0000479 denotes Tissue
T10934 4546-4556 GO:0042571 denotes antibodies
T12667 4677-4682 PR_EXT:000009158 denotes ITPR1
T12668 4702-4705 CHEBI_SO_EXT:DNA denotes DNA
T12669 4725-4728 NCBITaxon:10376 denotes EBV
T12670 4742-4753 CL:0000542 denotes lymphocytes
T12671 4788-4800 SO_EXT:0000195 denotes coding exons
T12672 4817-4819 SO_EXT:0000028 denotes bp
T12673 4823-4831 SO:0000357 denotes flanking
T12674 4832-4839 SO_EXT:0000188 denotes introns
T12675 4843-4848 PR_EXT:000009158 denotes ITPR1
T12676 4888-4891 CHEBI_EXT:37958 denotes dye
T12677 4990-4998 SO_EXT:biological_sequence denotes Sequence
T12678 5189-5200 SO_EXT:genomic_DNA denotes genomic DNA
T12679 5197-5200 CHEBI_SO_EXT:DNA denotes DNA
T12680 5239-5245 SO_EXT:0000112 denotes Primer
T12681 5246-5255 SO_EXT:biological_sequence denotes sequences
T12682 5299-5307 SO_EXT:biological_sequence denotes Sequence
T12683 5345-5349 SO_EXT:0000704 denotes Gene
T12684 5396-5402 SO_EXT:0001026 denotes Genome
T12685 5408-5411 SO_EXT:0000694 denotes SNP
T12686 5464-5467 SO_EXT:0000694 denotes SNP
T12687 5592-5596 SO_EXT:0000694 denotes SNPs
T12688 5687-5696 SO_EXT:genotype_or_entity_with_genotype denotes Genotypes
T12689 5803-5809 SO_EXT:0001023 denotes allele
T12690 5846-5852 SO_EXT:0001026 denotes genome
T12691 5941-5949 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12692 5957-5962 PR_EXT:000009158 denotes ITPR1
T12693 5976-5980 SO_EXT:sequence_copy_entity denotes copy
T12694 5988-5995 SO_EXT:sequence_variant_entity denotes variant
T12695 6026-6032 SO_EXT:0001023 denotes allele
T12696 6105-6116 NCBITaxon:1 denotes individuals
T12697 6269-6277 SO:0000357 denotes flanking
T12698 6282-6290 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12699 6328-6335 SO_EXT:0000112 denotes primers
T12700 6419-6436 SO_EXT:0000239 denotes bordering regions
T12701 6438-6444 SO_EXT:0000112 denotes primer
T12702 6445-6453 SO_EXT:biological_sequence denotes sequence
T12703 6509-6515 SO_EXT:0000112 denotes primer
T12704 6529-6538 GO_SO_EXT:telomere_or_telomeric_region_or_telomeric_orientation denotes telomeric
T12705 6539-6554 SO_EXT:0000239 denotes flanking region
T12706 6575-6586 GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation denotes centromeric
T12707 6587-6602 SO_EXT:0000239 denotes flanking region
T12708 6649-6651 SO_EXT:0000028 denotes bp
T12709 6701-6712 SO_EXT:genomic_DNA denotes genomic DNA
T12710 6709-6712 CHEBI_SO_EXT:DNA denotes DNA
T12711 6745-6756 NCBITaxon:1 denotes individuals
T12712 6788-6791 CHEBI_EXT:37958 denotes Dye
T12713 6854-6861 _FRAGMENT denotes forward
T12714 6874-6881 SO_EXT:0000121 denotes primers
T12715 6866-6881 SO_EXT:0000132 denotes reverse primers
T12716 7032-7038 SO_EXT:0001026 denotes genome
T12717 7085-7097 SO_EXT:polymorphism denotes polymorphism
T12718 7148-7154 SO_EXT:0001026 denotes genome
T12719 7180-7187 SO_EXT:0001026 denotes genomic
T12720 7188-7196 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12721 7242-7248 SO_EXT:0001023 denotes allele
T12722 7263-7269 SO_EXT:0001023 denotes allele
T12723 7387-7398 NCBITaxon:1 denotes individuals
T12724 7423-7432 SO_EXT:0001024 denotes haplotype
T12725 7537-7545 SO:0000357 denotes flanking
T12726 7550-7558 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12727 7566-7567 CHEBI_SO_EXT:base denotes b
T12728 7575-7584 GO_SO_EXT:telomere_or_telomeric_region_or_telomeric_orientation denotes telomeric
T12729 7597-7598 CHEBI_SO_EXT:base denotes b
T12730 7606-7617 GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation denotes centromeric
T12731 7644-7659 SO_EXT:0000121 denotes forward primers
T12732 7690-7698 SO:0000357 denotes flanking
T12733 7703-7711 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12734 7719-7728 GO_SO_EXT:telomere_or_telomeric_region_or_telomeric_orientation denotes telomeric
T12735 7739-7754 SO_EXT:0000132 denotes reverse primers
T12736 7785-7793 SO:0000357 denotes flanking
T12737 7798-7806 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T12738 7814-7825 GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation denotes centromeric
T12739 7887-7890 CHEBI_SO_EXT:DNA denotes DNA
T12740 8034-8041 SO_EXT:sequence_deletion_process denotes deleted
T12741 8072-8082 SO_EXT:0001021 denotes breakpoint
T12742 8130-8144 SO_EXT:0000121 denotes forward primer
T12743 8154-8163 GO_SO_EXT:telomere_or_telomeric_region_or_telomeric_orientation denotes telomeric
T12744 8216-8230 SO_EXT:0000132 denotes reverse primer
T12745 8240-8251 GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation denotes centromeric
T12746 8319-8321 SO_EXT:0000028 denotes bp
T12747 8405-8410 NCBITaxon:9606 denotes human
T12748 8411-8417 SO_EXT:0001026 denotes genome
T12749 8481-8501 SO_EXT:0001415 denotes deletion breakpoints
T12750 8557-8559 SO_EXT:0000028 denotes bp
T12751 8560-8571 SO_EXT:0000006 denotes PCR product
T12752 8583-8593 SO_EXT:0001021 denotes breakpoint
T12753 8640-8646 SO_EXT:0000112 denotes primer
T12754 8788-8798 SO_EXT:0001021 denotes breakpoint
T13790 8856-8859 NCBITaxon:10376 denotes EBV
T13791 8935-8943 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T13792 8978-8986 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T13793 9031-9038 CHEBI_PR_EXT:protein denotes protein
T13794 9086-9093 CHEBI_PR_EXT:protein denotes Protein
T13795 9125-9130 GO:0019835 denotes lysis
T13796 9131-9137 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T13797 9160-9172 CHEBI:9750 denotes Triton X-100
T13798 9180-9188 CHEBI:60004 denotes cocktail
T13799 9192-9211 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T13800 9235-9240 GO:0019835 denotes lysis
T13801 9306-9314 MOP:0000569 denotes reducing
T13802 9322-9328 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T13803 9400-9403 CHEBI:8984 denotes SDS
T13804 9433-9443 GO:0042571 denotes antibodies
T13805 9447-9452 PR_EXT:000009158 denotes ITPR1
T13806 9467-9471 PR_EXT:000003676 denotes ACTB
R2708 T9573 T9572 _lexicallyChainedTo sequence,flanking
R3354 T12714 T12713 _lexicallyChainedTo primers,forward

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T8940 23-29 SO:0001026 denotes Genome
T8941 46-50 NCBITaxon:10088 denotes mice
T8942 115-121 SO:0001026 denotes genome
T8943 176-180 SO:0000694 denotes SNPs
T8944 311-315 NCBITaxon:10088 denotes mice
T8945 336-340 NCBITaxon:10088 denotes mice
T8946 376-379 CHEBI:37958 denotes dye
T8947 764-768 NCBITaxon:10088 denotes mice
T8948 797-801 NCBITaxon:10088 denotes mice
T8949 851-859 SO:0000357 denotes flanking
T8950 986-991 SO:0000704 denotes genes
T8951 996-1017 SO:0002138 denotes predicted transcripts
T9530 1053-1060 SO:0000704 denotes genetic
T9531 1071-1075 NCBITaxon:10088 denotes mice
T9532 1117-1121 NCBITaxon:10088 denotes mice
T9533 1206-1211 PR:000009158 denotes Itpr1
T9534 1219-1224 NCBITaxon:10088 denotes mouse
T9535 1288-1293 SO:0000147 denotes exons
T9536 1307-1312 PR:000009158 denotes Itpr1
T9537 1314-1320 SO:0000112 denotes Primer
T9538 1365-1377 SO:0000195 denotes coding exons
T9539 1394-1396 SO:0000028 denotes bp
T9540 1405-1413 _FRAGMENT denotes flanking
T9541 1423-1431 SO:0000239 denotes sequence
T9542 1414-1422 SO:0000188 denotes intronic
T9543 1435-1440 PR:000009158 denotes Itpr1
T9544 1468-1472 SO:0000147 denotes exon
T9545 1515-1519 NCBITaxon:10088 denotes mice
T9546 1538-1543 PR:000009158 denotes Itpr1
T9547 1604-1608 NCBITaxon:10088 denotes mice
T9548 1677-1681 NCBITaxon:10088 denotes mice
T9549 1721-1726 PR:000009158 denotes Itpr1
T9550 1745-1750 NCBITaxon:10088 denotes mouse
T9551 1772-1777 PR:000009158 denotes Itpr1
T9552 1791-1796 PR:000009158 denotes Itpr1
T9553 1837-1841 NCBITaxon:10088 denotes mice
T9554 1872-1877 PR:000009158 denotes Itpr1
T9555 1935-1941 GO:0007618 denotes mating
T9556 1972-1978 GO:0007618 denotes mating
T9557 2032-2037 PR:000009158 denotes Itpr1
T9558 2049-2054 PR:000009158 denotes Itpr1
T9559 2063-2067 NCBITaxon:10088 denotes mice
T10079 2082-2087 PR:000009158 denotes Itpr1
T10080 2099-2103 NCBITaxon:10088 denotes mice
T10081 2252-2258 UBERON:0000955 denotes brains
T10082 2268-2273 GO:0007567 denotes natal
T10083 2339-2343 CHEBI:9754 denotes Tris
T10084 2344-2347 CHEBI:17883 denotes HCl
T10085 2356-2360 CHEBI:26710 denotes NaCl
T10086 2376-2388 CHEBI:9750 denotes Triton X-100
T10087 2393-2412 CHEBI:9177 denotes sodium deoxycholate
T10088 2419-2422 CHEBI:8984 denotes SDS
T10089 2430-2438 CHEBI:60004 denotes cocktail
T10090 2547-2555 MOP:0000569 denotes reducing
T10091 2655-2658 CHEBI:8984 denotes SDS
T10092 2688-2698 GO:0042571 denotes antibodies
T10093 2702-2707 PR:000009158 denotes Itpr1
T10094 2722-2726 PR:000003676 denotes Actb
T10864 2803-2809 UBERON:0000955 denotes Brains
T10865 2838-2842 NCBITaxon:10088 denotes mice
T10866 2923-2931 CHEBI:50913 denotes fixative
T10867 2933-2939 UBERON:0000955 denotes Brains
T10868 2957-2964 CHEBI:5291 denotes gelatin
T10869 3149-3155 UBERON:0000955 denotes brains
T10870 3180-3185 UBERON:0000955 denotes Brain
T10871 3263-3271 CHEBI:75958 denotes solution
T10872 3306-3310 NCBITaxon:9925 denotes goat
T10873 3311-3316 UBERON:0001977 denotes serum
T10874 3326-3338 CHEBI:9750 denotes Triton X-100
T10875 3402-3412 GO:0042571 denotes antibodies
T10876 3443-3448 PR:000009158 denotes Itpr1
T10877 3449-3457 GO:0042571 denotes antibody
T10878 3537-3542 PR:000004967 denotes Calb1
T10879 3543-3551 GO:0042571 denotes antibody
T10880 3625-3633 CHEBI:75958 denotes solution
T10881 3746-3756 GO:0042571 denotes antibodies
T10882 3758-3773 CHEBI:52673 denotes Alexa Fluor 555
T10883 3774-3778 NCBITaxon:9925 denotes goat
T10884 3784-3790 NCBITaxon:9986 denotes rabbit
T10885 3791-3794 GO:0071735 denotes IgG
T10886 3799-3814 CHEBI:52661 denotes Alexa Fluor 488
T10887 3815-3819 NCBITaxon:9925 denotes goat
T10888 3825-3830 NCBITaxon:10088 denotes mouse
T10889 3831-3834 GO:0071735 denotes IgG
T10890 4319-4325 UBERON:0000955 denotes brains
T10891 4346-4351 PR:000009158 denotes Itpr1
T10892 4352-4362 GO:0042571 denotes antibodies
T10893 4414-4422 GO:0042571 denotes Antibody
T10894 4494-4500 UBERON:0000479 denotes Tissue
T10895 4546-4556 GO:0042571 denotes antibodies
T12608 4677-4682 PR:000009158 denotes ITPR1
T12609 4725-4728 NCBITaxon:10376 denotes EBV
T12610 4742-4753 CL:0000542 denotes lymphocytes
T12611 4788-4800 SO:0000195 denotes coding exons
T12612 4817-4819 SO:0000028 denotes bp
T12613 4823-4831 SO:0000357 denotes flanking
T12614 4832-4839 SO:0000188 denotes introns
T12615 4843-4848 PR:000009158 denotes ITPR1
T12616 4888-4891 CHEBI:37958 denotes dye
T12617 5189-5196 SO:0001026 denotes genomic
T12618 5239-5245 SO:0000112 denotes Primer
T12619 5345-5349 SO:0000704 denotes Gene
T12620 5396-5402 SO:0001026 denotes Genome
T12621 5408-5411 SO:0000694 denotes SNP
T12622 5464-5467 SO:0000694 denotes SNP
T12623 5592-5596 SO:0000694 denotes SNPs
T12624 5803-5809 SO:0001023 denotes allele
T12625 5846-5852 SO:0001026 denotes genome
T12626 5957-5962 PR:000009158 denotes ITPR1
T12627 6026-6032 SO:0001023 denotes allele
T12628 6105-6116 NCBITaxon:1 denotes individuals
T12629 6269-6277 SO:0000357 denotes flanking
T12630 6328-6335 SO:0000112 denotes primers
T12631 6419-6436 SO:0000239 denotes bordering regions
T12632 6438-6444 SO:0000112 denotes primer
T12633 6509-6515 SO:0000112 denotes primer
T12634 6539-6554 SO:0000239 denotes flanking region
T12635 6587-6602 SO:0000239 denotes flanking region
T12636 6649-6651 SO:0000028 denotes bp
T12637 6701-6708 SO:0001026 denotes genomic
T12638 6745-6756 NCBITaxon:1 denotes individuals
T12639 6788-6791 CHEBI:37958 denotes Dye
T12640 6854-6861 _FRAGMENT denotes forward
T12641 6874-6881 SO:0000121 denotes primers
T12642 6866-6881 SO:0000132 denotes reverse primers
T12643 7032-7038 SO:0001026 denotes genome
T12644 7148-7154 SO:0001026 denotes genome
T12645 7180-7187 SO:0001026 denotes genomic
T12646 7242-7248 SO:0001023 denotes allele
T12647 7263-7269 SO:0001023 denotes allele
T12648 7387-7398 NCBITaxon:1 denotes individuals
T12649 7423-7432 SO:0001024 denotes haplotype
T12650 7537-7545 SO:0000357 denotes flanking
T12651 7644-7659 SO:0000121 denotes forward primers
T12652 7690-7698 SO:0000357 denotes flanking
T12653 7739-7754 SO:0000132 denotes reverse primers
T12654 7785-7793 SO:0000357 denotes flanking
T12655 8072-8082 SO:0001021 denotes breakpoint
T12656 8130-8144 SO:0000121 denotes forward primer
T12657 8216-8230 SO:0000132 denotes reverse primer
T12658 8319-8321 SO:0000028 denotes bp
T12659 8405-8410 NCBITaxon:9606 denotes human
T12660 8411-8417 SO:0001026 denotes genome
T12661 8481-8501 SO:0001415 denotes deletion breakpoints
T12662 8557-8559 SO:0000028 denotes bp
T12663 8560-8571 SO:0000006 denotes PCR product
T12664 8583-8593 SO:0001021 denotes breakpoint
T12665 8640-8646 SO:0000112 denotes primer
T12666 8788-8798 SO:0001021 denotes breakpoint
T13780 8856-8859 NCBITaxon:10376 denotes EBV
T13781 9125-9130 GO:0019835 denotes lysis
T13782 9160-9172 CHEBI:9750 denotes Triton X-100
T13783 9180-9188 CHEBI:60004 denotes cocktail
T13784 9235-9240 GO:0019835 denotes lysis
T13785 9306-9314 MOP:0000569 denotes reducing
T13786 9400-9403 CHEBI:8984 denotes SDS
T13787 9433-9443 GO:0042571 denotes antibodies
T13788 9447-9452 PR:000009158 denotes ITPR1
T13789 9467-9471 PR:000003676 denotes ACTB
R2707 T9541 T9540 _lexicallyChainedTo sequence,flanking
R3353 T12641 T12640 _lexicallyChainedTo primers,forward

craft-sa-dev

Id Subject Object Predicate Lexical cue
T8971 23-29 NN denotes Genome
T8972 30-34 JJ denotes wide
T8973 29-30 HYPH denotes -
T8974 35-42 NN denotes linkage
T8975 43-45 IN denotes in
T8976 46-50 NNS denotes mice
T8977 50-51 . denotes .
T8978 51-257 sentence denotes One hundred and twenty DNA fragments were amplified across the genome, each selected to contain one or more strain-specific SNPs that would differentiate between C57BL/6J and 129x1/SvJ inbred strains [12].
T8979 52-55 CD denotes One
T8980 56-63 CD denotes hundred
T8981 79-88 NNS denotes fragments
T8982 64-67 CC denotes and
T8983 68-74 CD denotes twenty
T8984 75-78 NN denotes DNA
T8985 94-103 VBN denotes amplified
T8986 89-93 VBD denotes were
T8987 104-110 IN denotes across
T8988 111-114 DT denotes the
T8989 115-121 NN denotes genome
T8990 121-123 , denotes ,
T8991 123-127 DT denotes each
T8992 128-136 VBN denotes selected
T8993 137-139 TO denotes to
T8994 140-147 VB denotes contain
T8995 148-151 CD denotes one
T8996 176-180 NNS denotes SNPs
T8997 152-154 CC denotes or
T8998 155-159 JJR denotes more
T8999 160-166 NN denotes strain
T9000 167-175 JJ denotes specific
T9001 166-167 HYPH denotes -
T9002 181-185 WDT denotes that
T9003 192-205 VB denotes differentiate
T9004 186-191 MD denotes would
T9005 206-213 IN denotes between
T9006 214-219 NN denotes C57BL
T9007 220-222 NN denotes 6J
T9008 219-220 HYPH denotes /
T9009 244-251 NNS denotes strains
T9010 223-226 CC denotes and
T9011 227-232 NN denotes 129x1
T9012 233-236 NN denotes SvJ
T9013 232-233 HYPH denotes /
T9014 237-243 JJ denotes inbred
T9015 252-253 -LRB- denotes [
T9016 253-255 CD denotes 12
T9017 255-256 -RRB- denotes ]
T9018 256-257 . denotes .
T9019 257-421 sentence denotes Each fragment was initially amplified in 11 affected mice and nine unaffected mice; genotype calling was performed by dye-terminator sequencing of these fragments.
T9020 258-262 DT denotes Each
T9021 263-271 NN denotes fragment
T9022 286-295 VBN denotes amplified
T9023 272-275 VBD denotes was
T9024 276-285 RB denotes initially
T9025 363-372 VBN denotes performed
T9026 296-298 IN denotes in
T9027 299-301 CD denotes 11
T9028 311-315 NNS denotes mice
T9029 302-310 VBN denotes affected
T9030 316-319 CC denotes and
T9031 320-324 CD denotes nine
T9032 336-340 NNS denotes mice
T9033 325-335 JJ denotes unaffected
T9034 340-341 : denotes ;
T9035 342-350 NN denotes genotype
T9036 351-358 NN denotes calling
T9037 359-362 VBD denotes was
T9038 373-375 IN denotes by
T9039 376-379 NN denotes dye
T9040 380-390 NN denotes terminator
T9041 379-380 HYPH denotes -
T9042 391-401 NN denotes sequencing
T9043 402-404 IN denotes of
T9044 405-410 DT denotes these
T9045 411-420 NNS denotes fragments
T9046 420-421 . denotes .
T9047 421-622 sentence denotes Linkage analysis using these data was performed using mlink [13], which revealed a positive linkage at Chromosome 6qE1, on the 129x1/SvJ background (two-point LOD score 5.13 at marker 20.MMHAP85FLG2).
T9048 422-429 NN denotes Linkage
T9049 430-438 NN denotes analysis
T9050 460-469 VBN denotes performed
T9051 439-444 VBG denotes using
T9052 445-450 DT denotes these
T9053 451-455 NNS denotes data
T9054 456-459 VBD denotes was
T9055 470-475 VBG denotes using
T9056 476-481 NN denotes mlink
T9057 482-483 -LRB- denotes [
T9058 483-485 CD denotes 13
T9059 485-486 -RRB- denotes ]
T9060 486-488 , denotes ,
T9061 488-493 WDT denotes which
T9062 494-502 VBD denotes revealed
T9063 503-504 DT denotes a
T9064 514-521 NN denotes linkage
T9065 505-513 JJ denotes positive
T9066 522-524 IN denotes at
T9067 525-535 NN denotes Chromosome
T9068 536-540 NN denotes 6qE1
T9069 540-542 , denotes ,
T9070 542-544 IN denotes on
T9071 545-548 DT denotes the
T9072 559-569 NN denotes background
T9073 549-554 NN denotes 129x1
T9074 555-558 NN denotes SvJ
T9075 554-555 HYPH denotes /
T9076 570-571 -LRB- denotes (
T9077 585-590 NN denotes score
T9078 571-574 CD denotes two
T9079 575-580 NN denotes point
T9080 574-575 HYPH denotes -
T9081 581-584 NN denotes LOD
T9082 591-595 CD denotes 5.13
T9083 596-598 IN denotes at
T9084 599-605 NN denotes marker
T9085 606-620 NN denotes 20.MMHAP85FLG2
T9086 620-621 -RRB- denotes )
T9087 621-622 . denotes .
T9088 622-769 sentence denotes In an attempt to narrow the disease interval we performed backcross experiments that resulted in the generation of three additional affected mice.
T9089 623-625 IN denotes In
T9090 671-680 VBD denotes performed
T9091 626-628 DT denotes an
T9092 629-636 NN denotes attempt
T9093 637-639 TO denotes to
T9094 640-646 VB denotes narrow
T9095 647-650 DT denotes the
T9096 659-667 NN denotes interval
T9097 651-658 NN denotes disease
T9098 668-670 PRP denotes we
T9099 681-690 NN denotes backcross
T9100 691-702 NNS denotes experiments
T9101 703-707 WDT denotes that
T9102 708-716 VBD denotes resulted
T9103 717-719 IN denotes in
T9104 720-723 DT denotes the
T9105 724-734 NN denotes generation
T9106 735-737 IN denotes of
T9107 738-743 CD denotes three
T9108 764-768 NNS denotes mice
T9109 744-754 JJ denotes additional
T9110 755-763 VBN denotes affected
T9111 768-769 . denotes .
T9112 769-961 sentence denotes Genotyping of all affected mice across the disease-segregating interval revealed flanking recombinants and a candidate region of ~5 Mb, between markers D6Mit37 and 44.MMHAP85FLG5 (Figure S1).
T9113 770-780 NN denotes Genotyping
T9114 842-850 VBD denotes revealed
T9115 781-783 IN denotes of
T9116 784-787 DT denotes all
T9117 797-801 NNS denotes mice
T9118 788-796 VBN denotes affected
T9119 802-808 IN denotes across
T9120 809-812 DT denotes the
T9121 833-841 NN denotes interval
T9122 813-820 NN denotes disease
T9123 821-832 VBG denotes segregating
T9124 820-821 HYPH denotes -
T9125 851-859 VBG denotes flanking
T9126 860-872 NNS denotes recombinants
T9127 873-876 CC denotes and
T9128 877-878 DT denotes a
T9129 889-895 NN denotes region
T9130 879-888 NN denotes candidate
T9131 896-898 IN denotes of
T9132 899-900 SYM denotes ~
T9133 900-901 CD denotes 5
T9134 902-904 NN denotes Mb
T9135 904-906 , denotes ,
T9136 906-913 IN denotes between
T9137 914-921 NNS denotes markers
T9138 922-929 NN denotes D6Mit37
T9139 930-933 CC denotes and
T9140 934-948 NN denotes 44.MMHAP85FLG5
T9141 949-950 -LRB- denotes (
T9142 957-959 NN denotes S1
T9143 950-956 NN denotes Figure
T9144 959-960 -RRB- denotes )
T9145 960-961 . denotes .
T9146 961-1018 sentence denotes This region contains 16 genes and predicted transcripts.
T9147 962-966 DT denotes This
T9148 967-973 NN denotes region
T9149 974-982 VBZ denotes contains
T9150 983-985 CD denotes 16
T9151 986-991 NNS denotes genes
T9152 992-995 CC denotes and
T9153 996-1005 VBN denotes predicted
T9154 1006-1017 NNS denotes transcripts
T9155 1017-1018 . denotes .
T9602 1020-1034 NN denotes Identification
T9603 1035-1037 IN denotes of
T9604 1038-1041 DT denotes the
T9605 1061-1067 NN denotes lesion
T9606 1042-1052 VBG denotes underlying
T9607 1053-1060 JJ denotes genetic
T9608 1068-1070 IN denotes in
T9609 1071-1075 NNS denotes mice
T9610 1075-1076 . denotes .
T9611 1076-1187 sentence denotes Identification of similar phenotypes in mice linked to the 6qE1 interval was performed by literature searches.
T9612 1077-1091 NN denotes Identification
T9613 1154-1163 VBN denotes performed
T9614 1092-1094 IN denotes of
T9615 1095-1102 JJ denotes similar
T9616 1103-1113 NNS denotes phenotypes
T9617 1114-1116 IN denotes in
T9618 1117-1121 NNS denotes mice
T9619 1122-1128 VBN denotes linked
T9620 1129-1131 IN denotes to
T9621 1132-1135 DT denotes the
T9622 1141-1149 NN denotes interval
T9623 1136-1140 NN denotes 6qE1
T9624 1150-1153 VBD denotes was
T9625 1164-1166 IN denotes by
T9626 1167-1177 NN denotes literature
T9627 1178-1186 NNS denotes searches
T9628 1186-1187 . denotes .
T9629 1187-1313 sentence denotes This revealed the Itpr1opt/opt mouse, in which disease is caused by homozygous deletion mutation of exons 43 and 44 of Itpr1.
T9630 1188-1192 DT denotes This
T9631 1193-1201 VBD denotes revealed
T9632 1202-1205 DT denotes the
T9633 1219-1224 NN denotes mouse
T9634 1206-1214 NN denotes Itpr1opt
T9635 1215-1218 NN denotes opt
T9636 1214-1215 HYPH denotes /
T9637 1224-1226 , denotes ,
T9638 1226-1228 IN denotes in
T9639 1246-1252 VBN denotes caused
T9640 1229-1234 WDT denotes which
T9641 1235-1242 NN denotes disease
T9642 1243-1245 VBZ denotes is
T9643 1253-1255 IN denotes by
T9644 1256-1266 JJ denotes homozygous
T9645 1276-1284 NN denotes mutation
T9646 1267-1275 NN denotes deletion
T9647 1285-1287 IN denotes of
T9648 1288-1293 NNS denotes exons
T9649 1294-1296 CD denotes 43
T9650 1297-1300 CC denotes and
T9651 1301-1303 CD denotes 44
T9652 1304-1306 IN denotes of
T9653 1307-1312 NN denotes Itpr1
T9654 1312-1313 . denotes .
T9655 1313-1441 sentence denotes Primer pairs were designed to sequence each of the coding exons and at least 50 bp of each flanking intronic sequence of Itpr1.
T9656 1314-1320 NN denotes Primer
T9657 1321-1326 NNS denotes pairs
T9658 1332-1340 VBN denotes designed
T9659 1327-1331 VBD denotes were
T9660 1341-1343 TO denotes to
T9661 1344-1352 VB denotes sequence
T9662 1353-1357 DT denotes each
T9663 1358-1360 IN denotes of
T9664 1361-1364 DT denotes the
T9665 1372-1377 NNS denotes exons
T9666 1365-1371 NN denotes coding
T9667 1378-1381 CC denotes and
T9668 1382-1384 RB denotes at
T9669 1391-1393 CD denotes 50
T9670 1385-1390 RBS denotes least
T9671 1394-1396 NN denotes bp
T9672 1397-1399 IN denotes of
T9673 1400-1404 DT denotes each
T9674 1423-1431 NN denotes sequence
T9675 1405-1413 VBG denotes flanking
T9676 1414-1422 JJ denotes intronic
T9677 1432-1434 IN denotes of
T9678 1435-1440 NN denotes Itpr1
T9679 1440-1441 . denotes .
T9680 1441-1533 sentence denotes PCR amplification of each exon was performed using DNA from two affected mice as templates.
T9681 1442-1445 NN denotes PCR
T9682 1446-1459 NN denotes amplification
T9683 1477-1486 VBN denotes performed
T9684 1460-1462 IN denotes of
T9685 1463-1467 DT denotes each
T9686 1468-1472 NN denotes exon
T9687 1473-1476 VBD denotes was
T9688 1487-1492 VBG denotes using
T9689 1493-1496 NN denotes DNA
T9690 1497-1501 IN denotes from
T9691 1502-1505 CD denotes two
T9692 1515-1519 NNS denotes mice
T9693 1506-1514 VBN denotes affected
T9694 1520-1522 IN denotes as
T9695 1523-1532 NNS denotes templates
T9696 1532-1533 . denotes .
T9697 1533-1621 sentence denotes The Itpr1Δ18/Δ18 mutation was confirmed by sequencing in all affected mice (Figure S2).
T9698 1534-1537 DT denotes The
T9699 1551-1559 NN denotes mutation
T9700 1538-1546 NN denotes Itpr1Δ18
T9701 1547-1550 NN denotes Δ18
T9702 1546-1547 HYPH denotes /
T9703 1564-1573 VBN denotes confirmed
T9704 1560-1563 VBD denotes was
T9705 1574-1576 IN denotes by
T9706 1577-1587 NN denotes sequencing
T9707 1588-1590 IN denotes in
T9708 1591-1594 DT denotes all
T9709 1604-1608 NNS denotes mice
T9710 1595-1603 VBN denotes affected
T9711 1609-1610 -LRB- denotes (
T9712 1617-1619 NN denotes S2
T9713 1610-1616 NN denotes Figure
T9714 1619-1620 -RRB- denotes )
T9715 1620-1621 . denotes .
T9716 1621-1804 sentence denotes Breeding experiments were performed between two female mice heterozygous for the current mutation (Itpr1wt/Δ18) and a male mouse heterozygous for the Itpr1opt mutation (Itpr1wt/opt).
T9717 1622-1630 VBG denotes Breeding
T9718 1631-1642 NNS denotes experiments
T9719 1648-1657 VBN denotes performed
T9720 1643-1647 VBD denotes were
T9721 1658-1665 IN denotes between
T9722 1666-1669 CD denotes two
T9723 1677-1681 NNS denotes mice
T9724 1670-1676 JJ denotes female
T9725 1682-1694 JJ denotes heterozygous
T9726 1695-1698 IN denotes for
T9727 1699-1702 DT denotes the
T9728 1711-1719 NN denotes mutation
T9729 1703-1710 JJ denotes current
T9730 1720-1721 -LRB- denotes (
T9731 1729-1732 NN denotes Δ18
T9732 1721-1728 NN denotes Itpr1wt
T9733 1728-1729 HYPH denotes /
T9734 1732-1733 -RRB- denotes )
T9735 1734-1737 CC denotes and
T9736 1738-1739 DT denotes a
T9737 1745-1750 NN denotes mouse
T9738 1740-1744 JJ denotes male
T9739 1751-1763 JJ denotes heterozygous
T9740 1764-1767 IN denotes for
T9741 1768-1771 DT denotes the
T9742 1781-1789 NN denotes mutation
T9743 1772-1780 NN denotes Itpr1opt
T9744 1790-1791 -LRB- denotes (
T9745 1799-1802 NN denotes opt
T9746 1791-1798 NN denotes Itpr1wt
T9747 1798-1799 HYPH denotes /
T9748 1802-1803 -RRB- denotes )
T9749 1803-1804 . denotes .
T9750 1804-2068 sentence denotes This resulted in two litters of mice with a total of four affected Itpr1opt/Δ18 pups (from a total of 15; two of seven from first mating; two of eight from the second mating) with a phenotype indistinguishable from that of the Itpr1Δ18/Δ18 and Itpr1opt /opt mice.
T9751 1805-1809 DT denotes This
T9752 1810-1818 VBD denotes resulted
T9753 1819-1821 IN denotes in
T9754 1822-1825 CD denotes two
T9755 1826-1833 NNS denotes litters
T9756 1834-1836 IN denotes of
T9757 1837-1841 NNS denotes mice
T9758 1842-1846 IN denotes with
T9759 1847-1848 DT denotes a
T9760 1849-1854 NN denotes total
T9761 1855-1857 IN denotes of
T9762 1858-1862 CD denotes four
T9763 1885-1889 NNS denotes pups
T9764 1863-1871 VBN denotes affected
T9765 1872-1880 NN denotes Itpr1opt
T9766 1881-1884 NN denotes Δ18
T9767 1880-1881 HYPH denotes /
T9768 1890-1891 -LRB- denotes (
T9769 1950-1955 CD denotes eight
T9770 1891-1895 IN denotes from
T9771 1896-1897 DT denotes a
T9772 1898-1903 NN denotes total
T9773 1904-1906 IN denotes of
T9774 1907-1909 CD denotes 15
T9775 1909-1910 : denotes ;
T9776 1911-1914 CD denotes two
T9777 1918-1923 CD denotes seven
T9778 1915-1917 IN denotes of
T9779 1924-1928 IN denotes from
T9780 1929-1934 JJ denotes first
T9781 1935-1941 NN denotes mating
T9782 1941-1942 : denotes ;
T9783 1943-1946 CD denotes two
T9784 1947-1949 IN denotes of
T9785 1956-1960 IN denotes from
T9786 1961-1964 DT denotes the
T9787 1972-1978 NN denotes mating
T9788 1965-1971 JJ denotes second
T9789 1978-1979 -RRB- denotes )
T9790 1980-1984 IN denotes with
T9791 1985-1986 DT denotes a
T9792 1987-1996 NN denotes phenotype
T9793 1997-2014 JJ denotes indistinguishable
T9794 2015-2019 IN denotes from
T9795 2020-2024 DT denotes that
T9796 2025-2027 IN denotes of
T9797 2028-2031 DT denotes the
T9798 2063-2067 NNS denotes mice
T9799 2032-2040 NN denotes Itpr1Δ18
T9800 2041-2044 NN denotes Δ18
T9801 2040-2041 HYPH denotes /
T9802 2045-2048 CC denotes and
T9803 2049-2057 NN denotes Itpr1opt
T9804 2059-2062 NN denotes opt
T9805 2058-2059 HYPH denotes /
T9806 2067-2068 . denotes .
T10116 2070-2078 NN denotes Analysis
T10117 2079-2081 IN denotes of
T10118 2082-2087 NN denotes Itpr1
T10119 2088-2095 NN denotes protein
T10120 2096-2098 IN denotes in
T10121 2099-2103 NNS denotes mice
T10122 2103-2104 . denotes .
T10123 2104-2226 sentence denotes We performed Western blot analyses using standard techniques with ECL detection kits (Amersham, http://www.amersham.com).
T10124 2105-2107 PRP denotes We
T10125 2108-2117 VBD denotes performed
T10126 2118-2125 NNP denotes Western
T10127 2126-2130 NN denotes blot
T10128 2131-2139 NNS denotes analyses
T10129 2140-2145 VBG denotes using
T10130 2146-2154 JJ denotes standard
T10131 2155-2165 NNS denotes techniques
T10132 2166-2170 IN denotes with
T10133 2171-2174 NN denotes ECL
T10134 2185-2189 NNS denotes kits
T10135 2175-2184 NN denotes detection
T10136 2190-2191 -LRB- denotes (
T10137 2191-2199 NNP denotes Amersham
T10138 2199-2201 , denotes ,
T10139 2201-2224 NN denotes http://www.amersham.com
T10140 2224-2225 -RRB- denotes )
T10141 2225-2226 . denotes .
T10142 2226-2492 sentence denotes Briefly, dissected whole brains from postnatal day 21 littermates were homogenized in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and a cocktail of protease inhibitors (Roche, http://www.roche.com).
T10143 2227-2234 RB denotes Briefly
T10144 2298-2309 VBN denotes homogenized
T10145 2234-2236 , denotes ,
T10146 2236-2245 VBN denotes dissected
T10147 2252-2258 NNS denotes brains
T10148 2246-2251 JJ denotes whole
T10149 2259-2263 IN denotes from
T10150 2264-2273 JJ denotes postnatal
T10151 2274-2277 NN denotes day
T10152 2281-2292 NNS denotes littermates
T10153 2278-2280 CD denotes 21
T10154 2293-2297 VBD denotes were
T10155 2310-2312 IN denotes in
T10156 2313-2314 DT denotes a
T10157 2315-2321 NN denotes buffer
T10158 2322-2332 VBG denotes containing
T10159 2333-2335 CD denotes 50
T10160 2336-2338 NN denotes mM
T10161 2344-2347 NN denotes HCl
T10162 2339-2343 NN denotes Tris
T10163 2343-2344 HYPH denotes -
T10164 2347-2349 , denotes ,
T10165 2349-2352 CD denotes 150
T10166 2353-2355 NN denotes mM
T10167 2356-2360 NN denotes NaCl
T10168 2360-2362 , denotes ,
T10169 2362-2363 CD denotes 1
T10170 2364-2366 NN denotes mM
T10171 2367-2371 NN denotes EDTA
T10172 2371-2373 , denotes ,
T10173 2373-2374 CD denotes 1
T10174 2374-2375 NN denotes %
T10175 2383-2384 NN denotes X
T10176 2376-2382 NN denotes Triton
T10177 2384-2385 HYPH denotes -
T10178 2385-2388 CD denotes 100
T10179 2388-2390 , denotes ,
T10180 2390-2391 CD denotes 1
T10181 2391-2392 NN denotes %
T10182 2400-2412 NN denotes deoxycholate
T10183 2393-2399 NN denotes sodium
T10184 2412-2414 , denotes ,
T10185 2414-2417 CD denotes 0.1
T10186 2417-2418 NN denotes %
T10187 2419-2422 NN denotes SDS
T10188 2422-2424 , denotes ,
T10189 2424-2427 CC denotes and
T10190 2428-2429 DT denotes a
T10191 2430-2438 NN denotes cocktail
T10192 2439-2441 IN denotes of
T10193 2442-2450 NN denotes protease
T10194 2451-2461 NNS denotes inhibitors
T10195 2462-2463 -LRB- denotes (
T10196 2463-2468 NNP denotes Roche
T10197 2468-2470 , denotes ,
T10198 2470-2490 NN denotes http://www.roche.com
T10199 2490-2491 -RRB- denotes )
T10200 2491-2492 . denotes .
T10201 2492-2683 sentence denotes Homogenates were diluted appropriately, mixed with 4× reducing sample buffer, and loaded onto 4%–12% precast gradient gels (Novex, http://www.invitrogen.com) for SDS-PAGE and immunoblotting.
T10202 2493-2504 NNS denotes Homogenates
T10203 2510-2517 VBN denotes diluted
T10204 2505-2509 VBD denotes were
T10205 2518-2531 RB denotes appropriately
T10206 2531-2533 , denotes ,
T10207 2533-2538 VBN denotes mixed
T10208 2539-2543 IN denotes with
T10209 2544-2545 CD denotes 4
T10210 2563-2569 NN denotes buffer
T10211 2545-2546 SYM denotes ×
T10212 2547-2555 VBG denotes reducing
T10213 2556-2562 NN denotes sample
T10214 2569-2571 , denotes ,
T10215 2571-2574 CC denotes and
T10216 2575-2581 VBN denotes loaded
T10217 2582-2586 IN denotes onto
T10218 2587-2588 CD denotes 4
T10219 2588-2589 NN denotes %
T10220 2611-2615 NNS denotes gels
T10221 2589-2590 SYM denotes –
T10222 2592-2593 NN denotes %
T10223 2590-2592 CD denotes 12
T10224 2594-2601 NN denotes precast
T10225 2602-2610 NN denotes gradient
T10226 2616-2617 -LRB- denotes (
T10227 2617-2622 NNP denotes Novex
T10228 2622-2624 , denotes ,
T10229 2624-2649 NN denotes http://www.invitrogen.com
T10230 2649-2650 -RRB- denotes )
T10231 2651-2654 IN denotes for
T10232 2655-2658 NN denotes SDS
T10233 2659-2663 NN denotes PAGE
T10234 2658-2659 HYPH denotes -
T10235 2664-2667 CC denotes and
T10236 2668-2682 NN denotes immunoblotting
T10237 2682-2683 . denotes .
T10238 2683-2779 sentence denotes The antibodies to Itpr1 (1:2,000) and Actb (1:5,000) were used as recommended by manufacturers.
T10239 2684-2687 DT denotes The
T10240 2688-2698 NNS denotes antibodies
T10241 2742-2746 VBN denotes used
T10242 2699-2701 IN denotes to
T10243 2702-2707 NN denotes Itpr1
T10244 2708-2709 -LRB- denotes (
T10245 2709-2710 CD denotes 1
T10246 2710-2711 SYM denotes :
T10247 2711-2716 CD denotes 2,000
T10248 2716-2717 -RRB- denotes )
T10249 2718-2721 CC denotes and
T10250 2722-2726 NN denotes Actb
T10251 2727-2728 -LRB- denotes (
T10252 2728-2729 CD denotes 1
T10253 2729-2730 SYM denotes :
T10254 2730-2735 CD denotes 5,000
T10255 2735-2736 -RRB- denotes )
T10256 2737-2741 VBD denotes were
T10257 2747-2749 IN denotes as
T10258 2750-2761 VBN denotes recommended
T10259 2762-2764 IN denotes by
T10260 2765-2778 NNS denotes manufacturers
T10261 2778-2779 . denotes .
T10935 2781-2801 NN denotes Immunohistochemistry
T10936 2801-2802 . denotes .
T10937 2802-2932 sentence denotes Brains were isolated from 21-d-old mice, perfused with 4% paraformaldehyde in PBS, and post-fixed overnight in the same fixative.
T10938 2803-2809 NNS denotes Brains
T10939 2815-2823 VBN denotes isolated
T10940 2810-2814 VBD denotes were
T10941 2824-2828 IN denotes from
T10942 2829-2831 CD denotes 21
T10943 2832-2833 NN denotes d
T10944 2831-2832 HYPH denotes -
T10945 2834-2837 JJ denotes old
T10946 2833-2834 HYPH denotes -
T10947 2838-2842 NNS denotes mice
T10948 2842-2844 , denotes ,
T10949 2844-2852 VBN denotes perfused
T10950 2853-2857 IN denotes with
T10951 2858-2859 CD denotes 4
T10952 2859-2860 NN denotes %
T10953 2861-2877 NN denotes paraformaldehyde
T10954 2878-2880 IN denotes in
T10955 2881-2884 NN denotes PBS
T10956 2884-2886 , denotes ,
T10957 2886-2889 CC denotes and
T10958 2890-2900 VBN denotes post-fixed
T10959 2901-2910 RB denotes overnight
T10960 2911-2913 IN denotes in
T10961 2914-2917 DT denotes the
T10962 2923-2931 NN denotes fixative
T10963 2918-2922 JJ denotes same
T10964 2931-2932 . denotes .
T10965 2932-3094 sentence denotes Brains were embedded in gelatin, and 35-μm sagittal sections were cut using a sliding microtome (NeuroScience Associates, http://www.neuroscienceassociates.com).
T10966 2933-2939 NNS denotes Brains
T10967 2945-2953 VBN denotes embedded
T10968 2940-2944 VBD denotes were
T10969 2954-2956 IN denotes in
T10970 2957-2964 NN denotes gelatin
T10971 2964-2966 , denotes ,
T10972 2966-2969 CC denotes and
T10973 2970-2972 CD denotes 35
T10974 2973-2975 NN denotes μm
T10975 2972-2973 HYPH denotes -
T10976 2985-2993 NNS denotes sections
T10977 2976-2984 JJ denotes sagittal
T10978 2999-3002 VBN denotes cut
T10979 2994-2998 VBD denotes were
T10980 3003-3008 VBG denotes using
T10981 3009-3010 DT denotes a
T10982 3019-3028 NN denotes microtome
T10983 3011-3018 VBG denotes sliding
T10984 3029-3030 -LRB- denotes (
T10985 3043-3053 NNP denotes Associates
T10986 3030-3042 NNP denotes NeuroScience
T10987 3053-3055 , denotes ,
T10988 3055-3092 NN denotes http://www.neuroscienceassociates.com
T10989 3092-3093 -RRB- denotes )
T10990 3093-3094 . denotes .
T10991 3094-3195 sentence denotes Sections from wild-type, heterozygote, and homozygous brains were placed in the MultiBrain template.
T10992 3095-3103 NNS denotes Sections
T10993 3161-3167 VBN denotes placed
T10994 3104-3108 IN denotes from
T10995 3109-3113 JJ denotes wild
T10996 3114-3118 NN denotes type
T10997 3113-3114 HYPH denotes -
T10998 3149-3155 NNS denotes brains
T10999 3118-3120 , denotes ,
T11000 3120-3132 NN denotes heterozygote
T11001 3132-3134 , denotes ,
T11002 3134-3137 CC denotes and
T11003 3138-3148 JJ denotes homozygous
T11004 3156-3160 VBD denotes were
T11005 3168-3170 IN denotes in
T11006 3171-3174 DT denotes the
T11007 3186-3194 NN denotes template
T11008 3175-3185 NN denotes MultiBrain
T11009 3194-3195 . denotes .
T11010 3195-3348 sentence denotes Sections were washed in 1× PBS prior to 1 h of incubation in block solution containing 1× PBS with 20% normal goat serum and 0.3% Triton X-100 (pH 7.4).
T11011 3196-3204 NNS denotes Sections
T11012 3210-3216 VBN denotes washed
T11013 3205-3209 VBD denotes were
T11014 3217-3219 IN denotes in
T11015 3220-3221 CD denotes 1
T11016 3223-3226 NN denotes PBS
T11017 3221-3222 SYM denotes ×
T11018 3227-3232 RB denotes prior
T11019 3233-3235 IN denotes to
T11020 3236-3237 CD denotes 1
T11021 3238-3239 NN denotes h
T11022 3240-3242 IN denotes of
T11023 3243-3253 NN denotes incubation
T11024 3254-3256 IN denotes in
T11025 3257-3262 NN denotes block
T11026 3263-3271 NN denotes solution
T11027 3272-3282 VBG denotes containing
T11028 3283-3284 CD denotes 1
T11029 3286-3289 NN denotes PBS
T11030 3284-3285 SYM denotes ×
T11031 3290-3294 IN denotes with
T11032 3295-3297 CD denotes 20
T11033 3297-3298 NN denotes %
T11034 3311-3316 NN denotes serum
T11035 3299-3305 JJ denotes normal
T11036 3306-3310 NN denotes goat
T11037 3317-3320 CC denotes and
T11038 3321-3324 CD denotes 0.3
T11039 3324-3325 NN denotes %
T11040 3333-3334 NN denotes X
T11041 3326-3332 NN denotes Triton
T11042 3334-3335 HYPH denotes -
T11043 3335-3338 CD denotes 100
T11044 3339-3340 -LRB- denotes (
T11045 3340-3342 NN denotes pH
T11046 3343-3346 CD denotes 7.4
T11047 3346-3347 -RRB- denotes )
T11048 3347-3348 . denotes .
T11049 3348-3634 sentence denotes Sections were incubated overnight at 4 °C in primary antibodies: affinity purified polyclonal Itpr1 antibody (1:2,000, Chemicon International, http://www.chemicon.com) and monoclonal anti-Calb1 antibody (1:6,000, Sigma-Aldrich, http://www.sigmaaldrich.com) diluted in carrier solution.
T11050 3349-3357 NNS denotes Sections
T11051 3363-3372 VBN denotes incubated
T11052 3358-3362 VBD denotes were
T11053 3373-3382 RB denotes overnight
T11054 3383-3385 IN denotes at
T11055 3386-3387 CD denotes 4
T11056 3388-3390 NN denotes °C
T11057 3391-3393 IN denotes in
T11058 3394-3401 JJ denotes primary
T11059 3402-3412 NNS denotes antibodies
T11060 3412-3414 : denotes :
T11061 3414-3422 NN denotes affinity
T11062 3449-3457 NN denotes antibody
T11063 3423-3431 VBN denotes purified
T11064 3432-3442 JJ denotes polyclonal
T11065 3443-3448 NN denotes Itpr1
T11066 3458-3459 -LRB- denotes (
T11067 3477-3490 NNP denotes International
T11068 3459-3460 CD denotes 1
T11069 3460-3461 SYM denotes :
T11070 3461-3466 CD denotes 2,000
T11071 3466-3468 , denotes ,
T11072 3468-3476 NNP denotes Chemicon
T11073 3490-3492 , denotes ,
T11074 3492-3515 NN denotes http://www.chemicon.com
T11075 3515-3516 -RRB- denotes )
T11076 3517-3520 CC denotes and
T11077 3521-3531 JJ denotes monoclonal
T11078 3543-3551 NN denotes antibody
T11079 3532-3542 JJ denotes anti-Calb1
T11080 3552-3553 -LRB- denotes (
T11081 3568-3575 NNP denotes Aldrich
T11082 3553-3554 CD denotes 1
T11083 3554-3555 SYM denotes :
T11084 3555-3560 CD denotes 6,000
T11085 3560-3562 , denotes ,
T11086 3562-3567 NNP denotes Sigma
T11087 3567-3568 HYPH denotes -
T11088 3575-3577 , denotes ,
T11089 3577-3604 NN denotes http://www.sigmaaldrich.com
T11090 3604-3605 -RRB- denotes )
T11091 3606-3613 VBN denotes diluted
T11092 3614-3616 IN denotes in
T11093 3617-3624 NN denotes carrier
T11094 3625-3633 NN denotes solution
T11095 3633-3634 . denotes .
T11096 3634-3904 sentence denotes Following extensive washes (in 6.0 ml of PBS, three times), sections were incubated with appropriate secondary antibodies (Alexa Fluor 555 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG [Invitrogen, http://www.invitrogen.com]) for 1 h at room temperature.
T11097 3635-3644 VBG denotes Following
T11098 3709-3718 VBN denotes incubated
T11099 3645-3654 JJ denotes extensive
T11100 3655-3661 NNS denotes washes
T11101 3662-3663 -LRB- denotes (
T11102 3663-3665 IN denotes in
T11103 3666-3669 CD denotes 6.0
T11104 3670-3672 NN denotes ml
T11105 3673-3675 IN denotes of
T11106 3676-3679 NN denotes PBS
T11107 3679-3681 , denotes ,
T11108 3687-3692 NNS denotes times
T11109 3681-3686 CD denotes three
T11110 3692-3693 -RRB- denotes )
T11111 3693-3695 , denotes ,
T11112 3695-3703 NNS denotes sections
T11113 3704-3708 VBD denotes were
T11114 3719-3723 IN denotes with
T11115 3724-3735 JJ denotes appropriate
T11116 3746-3756 NNS denotes antibodies
T11117 3736-3745 JJ denotes secondary
T11118 3757-3758 -LRB- denotes (
T11119 3791-3794 NN denotes IgG
T11120 3758-3763 NNP denotes Alexa
T11121 3764-3769 NNP denotes Fluor
T11122 3770-3773 CD denotes 555
T11123 3774-3778 NN denotes goat
T11124 3779-3790 JJ denotes anti-rabbit
T11125 3795-3798 CC denotes and
T11126 3799-3804 NNP denotes Alexa
T11127 3805-3810 NNP denotes Fluor
T11128 3831-3834 NN denotes IgG
T11129 3811-3814 CD denotes 488
T11130 3815-3819 NN denotes goat
T11131 3820-3830 JJ denotes anti-mouse
T11132 3835-3836 -LRB- denotes [
T11133 3836-3846 NNP denotes Invitrogen
T11134 3846-3848 , denotes ,
T11135 3848-3873 NN denotes http://www.invitrogen.com
T11136 3873-3874 -RRB- denotes ]
T11137 3874-3875 -RRB- denotes )
T11138 3876-3879 IN denotes for
T11139 3880-3881 CD denotes 1
T11140 3882-3883 NN denotes h
T11141 3884-3886 IN denotes at
T11142 3887-3891 NN denotes room
T11143 3892-3903 NN denotes temperature
T11144 3903-3904 . denotes .
T11145 3904-4063 sentence denotes Sections were washed and mounted on glass slides in a buffered medium containing Mowiol (Calbiochem, http://www.emdbiosciences.com) as described earlier [14].
T11146 3905-3913 NNS denotes Sections
T11147 3919-3925 VBN denotes washed
T11148 3914-3918 VBD denotes were
T11149 3926-3929 CC denotes and
T11150 3930-3937 VBN denotes mounted
T11151 3938-3940 IN denotes on
T11152 3941-3946 NN denotes glass
T11153 3947-3953 NNS denotes slides
T11154 3954-3956 IN denotes in
T11155 3957-3958 DT denotes a
T11156 3968-3974 NN denotes medium
T11157 3959-3967 VBN denotes buffered
T11158 3975-3985 VBG denotes containing
T11159 3986-3992 NN denotes Mowiol
T11160 3993-3994 -LRB- denotes (
T11161 3994-4004 NNP denotes Calbiochem
T11162 4004-4006 , denotes ,
T11163 4006-4035 NN denotes http://www.emdbiosciences.com
T11164 4035-4036 -RRB- denotes )
T11165 4037-4039 IN denotes as
T11166 4040-4049 VBN denotes described
T11167 4050-4057 RBR denotes earlier
T11168 4058-4059 -LRB- denotes [
T11169 4059-4061 CD denotes 14
T11170 4061-4062 -RRB- denotes ]
T11171 4062-4063 . denotes .
T11172 4063-4167 sentence denotes Sections were imaged using a laser scanning confocal microscope (LSM 510; Zeiss, http://www.zeiss.com).
T11173 4064-4072 NNS denotes Sections
T11174 4078-4084 VBN denotes imaged
T11175 4073-4077 VBD denotes were
T11176 4085-4090 VBG denotes using
T11177 4091-4092 DT denotes a
T11178 4117-4127 NN denotes microscope
T11179 4093-4098 NN denotes laser
T11180 4099-4107 VBG denotes scanning
T11181 4108-4116 JJ denotes confocal
T11182 4128-4129 -LRB- denotes (
T11183 4138-4143 NNP denotes Zeiss
T11184 4129-4132 NN denotes LSM
T11185 4133-4136 CD denotes 510
T11186 4136-4137 : denotes ;
T11187 4143-4145 , denotes ,
T11188 4145-4165 NN denotes http://www.zeiss.com
T11189 4165-4166 -RRB- denotes )
T11190 4166-4167 . denotes .
T11191 4167-4326 sentence denotes Imaging parameters (pinhole, detector gain, laser power) were optimized, and were kept constant for the wild-type, heterozygous, and homozygous mutant brains.
T11192 4168-4175 NN denotes Imaging
T11193 4176-4186 NNS denotes parameters
T11194 4230-4239 VBN denotes optimized
T11195 4187-4188 -LRB- denotes (
T11196 4188-4195 NN denotes pinhole
T11197 4195-4197 , denotes ,
T11198 4197-4205 NN denotes detector
T11199 4206-4210 NN denotes gain
T11200 4210-4212 , denotes ,
T11201 4212-4217 NN denotes laser
T11202 4218-4223 NN denotes power
T11203 4223-4224 -RRB- denotes )
T11204 4225-4229 VBD denotes were
T11205 4239-4241 , denotes ,
T11206 4241-4244 CC denotes and
T11207 4245-4249 VBD denotes were
T11208 4250-4254 VBN denotes kept
T11209 4255-4263 JJ denotes constant
T11210 4264-4267 IN denotes for
T11211 4268-4271 DT denotes the
T11212 4319-4325 NNS denotes brains
T11213 4272-4276 JJ denotes wild
T11214 4277-4281 NN denotes type
T11215 4276-4277 HYPH denotes -
T11216 4281-4283 , denotes ,
T11217 4283-4295 JJ denotes heterozygous
T11218 4295-4297 , denotes ,
T11219 4297-4300 CC denotes and
T11220 4301-4311 JJ denotes homozygous
T11221 4312-4318 NN denotes mutant
T11222 4325-4326 . denotes .
T11223 4326-4413 sentence denotes Specificity of the Itpr1 antibodies was verified by preabsorption control experiments.
T11224 4327-4338 NN denotes Specificity
T11225 4367-4375 VBN denotes verified
T11226 4339-4341 IN denotes of
T11227 4342-4345 DT denotes the
T11228 4352-4362 NNS denotes antibodies
T11229 4346-4351 NN denotes Itpr1
T11230 4363-4366 VBD denotes was
T11231 4376-4378 IN denotes by
T11232 4379-4392 NN denotes preabsorption
T11233 4401-4412 NNS denotes experiments
T11234 4393-4400 NN denotes control
T11235 4412-4413 . denotes .
T11236 4413-4493 sentence denotes Antibody dilutions were incubated for 24 h at 4 °C with the immunizing peptide.
T11237 4414-4422 NN denotes Antibody
T11238 4423-4432 NNS denotes dilutions
T11239 4438-4447 VBN denotes incubated
T11240 4433-4437 VBD denotes were
T11241 4448-4451 IN denotes for
T11242 4452-4454 CD denotes 24
T11243 4455-4456 NN denotes h
T11244 4457-4459 IN denotes at
T11245 4460-4461 CD denotes 4
T11246 4462-4464 NN denotes °C
T11247 4465-4469 IN denotes with
T11248 4470-4473 DT denotes the
T11249 4485-4492 NN denotes peptide
T11250 4474-4484 VBG denotes immunizing
T11251 4492-4493 . denotes .
T11252 4493-4590 sentence denotes Tissue sections were incubated with the preabsorbed antibodies and processed as described above.
T11253 4494-4500 NN denotes Tissue
T11254 4501-4509 NNS denotes sections
T11255 4515-4524 VBN denotes incubated
T11256 4510-4514 VBD denotes were
T11257 4525-4529 IN denotes with
T11258 4530-4533 DT denotes the
T11259 4546-4556 NNS denotes antibodies
T11260 4534-4545 VBN denotes preabsorbed
T11261 4557-4560 CC denotes and
T11262 4561-4570 VBN denotes processed
T11263 4571-4573 IN denotes as
T11264 4574-4583 VBN denotes described
T11265 4584-4589 RB denotes above
T11266 4589-4590 . denotes .
T11267 4590-4663 sentence denotes Under these conditions, no staining above autofluorescence was detected.
T11268 4591-4596 IN denotes Under
T11269 4654-4662 VBN denotes detected
T11270 4597-4602 DT denotes these
T11271 4603-4613 NNS denotes conditions
T11272 4613-4615 , denotes ,
T11273 4615-4617 DT denotes no
T11274 4618-4626 NN denotes staining
T11275 4627-4632 IN denotes above
T11276 4633-4649 NN denotes autofluorescence
T11277 4650-4653 VBD denotes was
T11278 4662-4663 . denotes .
T12755 4665-4673 NN denotes Analysis
T12756 4674-4676 IN denotes of
T12757 4677-4682 NN denotes ITPR1
T12758 4683-4685 IN denotes in
T12759 4686-4691 NN denotes SCA15
T12760 4692-4700 NNS denotes patients
T12761 4700-4701 . denotes .
T12762 4701-4783 sentence denotes DNA was extracted from EBV immortalized lymphocytes, derived from family members.
T12763 4702-4705 NN denotes DNA
T12764 4710-4719 VBN denotes extracted
T12765 4706-4709 VBD denotes was
T12766 4720-4724 IN denotes from
T12767 4725-4728 NN denotes EBV
T12768 4742-4753 NNS denotes lymphocytes
T12769 4729-4741 VBN denotes immortalized
T12770 4753-4755 , denotes ,
T12771 4755-4762 VBN denotes derived
T12772 4763-4767 IN denotes from
T12773 4768-4774 NN denotes family
T12774 4775-4782 NNS denotes members
T12775 4782-4783 . denotes .
T12776 4783-4989 sentence denotes The coding exons and at least 50 bp of flanking introns of ITPR1 were PCR amplified and sequenced using dye-terminator sequencing (BigDye version 3.1; Applied Biosystems, http://www.appliedbiosystems.com).
T12777 4784-4787 DT denotes The
T12778 4795-4800 NNS denotes exons
T12779 4788-4794 VBG denotes coding
T12780 4858-4867 VBN denotes amplified
T12781 4801-4804 CC denotes and
T12782 4805-4807 RB denotes at
T12783 4814-4816 CD denotes 50
T12784 4808-4813 RBS denotes least
T12785 4817-4819 NN denotes bp
T12786 4820-4822 IN denotes of
T12787 4823-4831 VBG denotes flanking
T12788 4832-4839 NNS denotes introns
T12789 4840-4842 IN denotes of
T12790 4843-4848 NN denotes ITPR1
T12791 4849-4853 VBD denotes were
T12792 4854-4857 NN denotes PCR
T12793 4868-4871 CC denotes and
T12794 4872-4881 VBN denotes sequenced
T12795 4882-4887 VBG denotes using
T12796 4888-4891 NN denotes dye
T12797 4892-4902 NN denotes terminator
T12798 4891-4892 HYPH denotes -
T12799 4903-4913 NN denotes sequencing
T12800 4914-4915 -LRB- denotes (
T12801 4943-4953 NNP denotes Biosystems
T12802 4915-4921 NNP denotes BigDye
T12803 4922-4929 NN denotes version
T12804 4930-4933 CD denotes 3.1
T12805 4933-4934 : denotes ;
T12806 4935-4942 NNP denotes Applied
T12807 4953-4955 , denotes ,
T12808 4955-4987 NN denotes http://www.appliedbiosystems.com
T12809 4987-4988 -RRB- denotes )
T12810 4988-4989 . denotes .
T12811 4989-5114 sentence denotes Sequence reactions were run on an ABI3730XP automated sequencer as per the manufacturer's instructions (Applied Biosystems).
T12812 4990-4998 NN denotes Sequence
T12813 4999-5008 NNS denotes reactions
T12814 5014-5017 VBN denotes run
T12815 5009-5013 VBD denotes were
T12816 5018-5020 IN denotes on
T12817 5021-5023 DT denotes an
T12818 5044-5053 NN denotes sequencer
T12819 5024-5033 NN denotes ABI3730XP
T12820 5034-5043 VBN denotes automated
T12821 5054-5056 IN denotes as
T12822 5057-5060 IN denotes per
T12823 5061-5064 DT denotes the
T12824 5065-5077 NN denotes manufacturer
T12825 5080-5092 NNS denotes instructions
T12826 5077-5079 POS denotes 's
T12827 5093-5094 -LRB- denotes (
T12828 5102-5112 NNP denotes Biosystems
T12829 5094-5101 NNP denotes Applied
T12830 5112-5113 -RRB- denotes )
T12831 5113-5114 . denotes .
T12832 5114-5238 sentence denotes This analysis was performed in all three affected family members for whom genomic DNA was available (members 6, 7, and 19).
T12833 5115-5119 DT denotes This
T12834 5120-5128 NN denotes analysis
T12835 5133-5142 VBN denotes performed
T12836 5129-5132 VBD denotes was
T12837 5143-5145 IN denotes in
T12838 5146-5149 DT denotes all
T12839 5172-5179 NNS denotes members
T12840 5150-5155 CD denotes three
T12841 5156-5164 VBN denotes affected
T12842 5165-5171 NN denotes family
T12843 5180-5183 IN denotes for
T12844 5201-5204 VBD denotes was
T12845 5184-5188 WP denotes whom
T12846 5189-5196 JJ denotes genomic
T12847 5197-5200 NN denotes DNA
T12848 5205-5214 JJ denotes available
T12849 5215-5216 -LRB- denotes (
T12850 5224-5225 CD denotes 6
T12851 5216-5223 NNS denotes members
T12852 5225-5227 , denotes ,
T12853 5227-5228 CD denotes 7
T12854 5228-5230 , denotes ,
T12855 5230-5233 CC denotes and
T12856 5234-5236 CD denotes 19
T12857 5236-5237 -RRB- denotes )
T12858 5237-5238 . denotes .
T12859 5238-5298 sentence denotes Primer sequences and conditions are available upon request.
T12860 5239-5245 NN denotes Primer
T12861 5246-5255 NNS denotes sequences
T12862 5271-5274 VBP denotes are
T12863 5256-5259 CC denotes and
T12864 5260-5270 NNS denotes conditions
T12865 5275-5284 JJ denotes available
T12866 5285-5289 IN denotes upon
T12867 5290-5297 NN denotes request
T12868 5297-5298 . denotes .
T12869 5298-5395 sentence denotes Sequence data were analyzed using Sequencher (Gene Codes Corporation, http://www.genecodes.com).
T12870 5299-5307 NN denotes Sequence
T12871 5308-5312 NNS denotes data
T12872 5318-5326 VBN denotes analyzed
T12873 5313-5317 VBD denotes were
T12874 5327-5332 VBG denotes using
T12875 5333-5343 NNP denotes Sequencher
T12876 5344-5345 -LRB- denotes (
T12877 5356-5367 NNP denotes Corporation
T12878 5345-5349 NNP denotes Gene
T12879 5350-5355 NNPS denotes Codes
T12880 5367-5369 , denotes ,
T12881 5369-5393 NN denotes http://www.genecodes.com
T12882 5393-5394 -RRB- denotes )
T12883 5394-5395 . denotes .
T12884 5395-5556 sentence denotes Genome-wide SNP genotyping was performed using Infinium HumanHap550 SNP genotyping chips as per the manufacturer's protocol (Illumina, http://www.illumina.com).
T12885 5396-5402 NN denotes Genome
T12886 5403-5407 JJ denotes wide
T12887 5402-5403 HYPH denotes -
T12888 5412-5422 NN denotes genotyping
T12889 5408-5411 NN denotes SNP
T12890 5427-5436 VBN denotes performed
T12891 5423-5426 VBD denotes was
T12892 5437-5442 VBG denotes using
T12893 5443-5451 NNP denotes Infinium
T12894 5464-5467 NN denotes SNP
T12895 5452-5463 NN denotes HumanHap550
T12896 5479-5484 NNS denotes chips
T12897 5468-5478 NN denotes genotyping
T12898 5485-5487 IN denotes as
T12899 5488-5491 IN denotes per
T12900 5492-5495 DT denotes the
T12901 5496-5508 NN denotes manufacturer
T12902 5511-5519 NN denotes protocol
T12903 5508-5510 POS denotes 's
T12904 5520-5521 -LRB- denotes (
T12905 5521-5529 NNP denotes Illumina
T12906 5529-5531 , denotes ,
T12907 5531-5554 NN denotes http://www.illumina.com
T12908 5554-5555 -RRB- denotes )
T12909 5555-5556 . denotes .
T12910 5556-5597 sentence denotes This product assays 555,352 unique SNPs.
T12911 5557-5561 DT denotes This
T12912 5562-5569 NN denotes product
T12913 5570-5576 VBZ denotes assays
T12914 5577-5584 CD denotes 555,352
T12915 5592-5596 NNS denotes SNPs
T12916 5585-5591 JJ denotes unique
T12917 5596-5597 . denotes .
T12918 5597-5686 sentence denotes Data were collected using the Illumina BeadStation scanner and data collection software.
T12919 5598-5602 NNS denotes Data
T12920 5608-5617 VBN denotes collected
T12921 5603-5607 VBD denotes were
T12922 5618-5623 VBG denotes using
T12923 5624-5627 DT denotes the
T12924 5649-5656 NN denotes scanner
T12925 5628-5636 NNP denotes Illumina
T12926 5637-5648 NNP denotes BeadStation
T12927 5657-5660 CC denotes and
T12928 5661-5665 NNS denotes data
T12929 5666-5676 NN denotes collection
T12930 5677-5685 NN denotes software
T12931 5685-5686 . denotes .
T12932 5686-5885 sentence denotes Genotypes were produced using the genotyping module of BeadStudio (version 2.3.25; Illumina), and log R ratio and B allele frequency were visualized using the genome viewer tool within this package.
T12933 5687-5696 NNS denotes Genotypes
T12934 5702-5710 VBN denotes produced
T12935 5697-5701 VBD denotes were
T12936 5711-5716 VBG denotes using
T12937 5717-5720 DT denotes the
T12938 5732-5738 NN denotes module
T12939 5721-5731 NN denotes genotyping
T12940 5739-5741 IN denotes of
T12941 5742-5752 NNP denotes BeadStudio
T12942 5753-5754 -LRB- denotes (
T12943 5754-5761 NN denotes version
T12944 5762-5768 CD denotes 2.3.25
T12945 5768-5769 : denotes ;
T12946 5770-5778 NNP denotes Illumina
T12947 5778-5779 -RRB- denotes )
T12948 5779-5781 , denotes ,
T12949 5781-5784 CC denotes and
T12950 5785-5788 NN denotes log
T12951 5789-5790 NN denotes R
T12952 5791-5796 NN denotes ratio
T12953 5825-5835 VBN denotes visualized
T12954 5797-5800 CC denotes and
T12955 5801-5802 NN denotes B
T12956 5803-5809 NN denotes allele
T12957 5810-5819 NN denotes frequency
T12958 5820-5824 VBD denotes were
T12959 5836-5841 VBG denotes using
T12960 5842-5845 DT denotes the
T12961 5860-5864 NN denotes tool
T12962 5846-5852 NN denotes genome
T12963 5853-5859 JJR denotes viewer
T12964 5865-5871 IN denotes within
T12965 5872-5876 DT denotes this
T12966 5877-5884 NN denotes package
T12967 5884-5885 . denotes .
T12968 5885-6222 sentence denotes In order to rule out the possibility that the observed deletion within ITPR1 was a benign copy number variant we examined log R ratio and B allele frequency metrics of HumanHap550 genotyping data at this locus from 577 individuals of Northern European descent from North America and Europe, produced by us as a part of an ongoing study.
T12969 5886-5888 IN denotes In
T12970 5999-6007 VBD denotes examined
T12971 5889-5894 NN denotes order
T12972 5895-5897 TO denotes to
T12973 5898-5902 VB denotes rule
T12974 5903-5906 RP denotes out
T12975 5907-5910 DT denotes the
T12976 5911-5922 NN denotes possibility
T12977 5923-5927 IN denotes that
T12978 5963-5966 VBD denotes was
T12979 5928-5931 DT denotes the
T12980 5941-5949 NN denotes deletion
T12981 5932-5940 VBN denotes observed
T12982 5950-5956 IN denotes within
T12983 5957-5962 NN denotes ITPR1
T12984 5967-5968 DT denotes a
T12985 5988-5995 NN denotes variant
T12986 5969-5975 JJ denotes benign
T12987 5976-5980 NN denotes copy
T12988 5981-5987 NN denotes number
T12989 5996-5998 PRP denotes we
T12990 6008-6011 NN denotes log
T12991 6012-6013 NN denotes R
T12992 6014-6019 NN denotes ratio
T12993 6043-6050 NNS denotes metrics
T12994 6020-6023 CC denotes and
T12995 6024-6025 NN denotes B
T12996 6026-6032 NN denotes allele
T12997 6033-6042 NN denotes frequency
T12998 6051-6053 IN denotes of
T12999 6054-6065 NN denotes HumanHap550
T13000 6077-6081 NNS denotes data
T13001 6066-6076 NN denotes genotyping
T13002 6082-6084 IN denotes at
T13003 6085-6089 DT denotes this
T13004 6090-6095 NN denotes locus
T13005 6096-6100 IN denotes from
T13006 6101-6104 CD denotes 577
T13007 6105-6116 NNS denotes individuals
T13008 6117-6119 IN denotes of
T13009 6120-6128 JJ denotes Northern
T13010 6129-6137 JJ denotes European
T13011 6138-6145 NN denotes descent
T13012 6146-6150 IN denotes from
T13013 6151-6156 NNP denotes North
T13014 6157-6164 NNP denotes America
T13015 6165-6168 CC denotes and
T13016 6169-6175 NNP denotes Europe
T13017 6175-6177 , denotes ,
T13018 6177-6185 VBN denotes produced
T13019 6186-6188 IN denotes by
T13020 6189-6191 PRP denotes us
T13021 6192-6194 IN denotes as
T13022 6195-6196 DT denotes a
T13023 6197-6201 NN denotes part
T13024 6202-6204 IN denotes of
T13025 6205-6207 DT denotes an
T13026 6216-6221 NN denotes study
T13027 6208-6215 JJ denotes ongoing
T13028 6221-6222 . denotes .
T13029 6222-6493 sentence denotes In an attempt to narrow the unknown intervals flanking the deletion observed in family AUS1, we designed primers for 30 PCR amplifications that would generate overlapping fragments across the two bordering regions (primer sequence and conditions available upon request).
T13030 6223-6225 IN denotes In
T13031 6319-6327 VBD denotes designed
T13032 6226-6228 DT denotes an
T13033 6229-6236 NN denotes attempt
T13034 6237-6239 TO denotes to
T13035 6240-6246 VB denotes narrow
T13036 6247-6250 DT denotes the
T13037 6259-6268 NNS denotes intervals
T13038 6251-6258 JJ denotes unknown
T13039 6269-6277 VBG denotes flanking
T13040 6278-6281 DT denotes the
T13041 6282-6290 NN denotes deletion
T13042 6291-6299 VBN denotes observed
T13043 6300-6302 IN denotes in
T13044 6303-6309 NN denotes family
T13045 6310-6314 NN denotes AUS1
T13046 6314-6316 , denotes ,
T13047 6316-6318 PRP denotes we
T13048 6328-6335 NNS denotes primers
T13049 6336-6339 IN denotes for
T13050 6340-6342 CD denotes 30
T13051 6347-6361 NNS denotes amplifications
T13052 6343-6346 NN denotes PCR
T13053 6362-6366 WDT denotes that
T13054 6373-6381 VB denotes generate
T13055 6367-6372 MD denotes would
T13056 6382-6393 VBG denotes overlapping
T13057 6394-6403 NNS denotes fragments
T13058 6404-6410 IN denotes across
T13059 6411-6414 DT denotes the
T13060 6429-6436 NNS denotes regions
T13061 6415-6418 CD denotes two
T13062 6419-6428 NN denotes bordering
T13063 6437-6438 -LRB- denotes (
T13064 6445-6453 NN denotes sequence
T13065 6438-6444 NN denotes primer
T13066 6454-6457 CC denotes and
T13067 6458-6468 NNS denotes conditions
T13068 6469-6478 JJ denotes available
T13069 6479-6483 IN denotes upon
T13070 6484-6491 NN denotes request
T13071 6491-6492 -RRB- denotes )
T13072 6492-6493 . denotes .
T13073 6493-6615 sentence denotes There were ten primer pairs in the telomeric flanking region and 20 pairs in the centromeric flanking region (Figure S3).
T13074 6494-6499 EX denotes There
T13075 6500-6504 VBD denotes were
T13076 6505-6508 CD denotes ten
T13077 6516-6521 NNS denotes pairs
T13078 6509-6515 NN denotes primer
T13079 6522-6524 IN denotes in
T13080 6525-6528 DT denotes the
T13081 6548-6554 NN denotes region
T13082 6529-6538 JJ denotes telomeric
T13083 6539-6547 NN denotes flanking
T13084 6555-6558 CC denotes and
T13085 6559-6561 CD denotes 20
T13086 6562-6567 NNS denotes pairs
T13087 6568-6570 IN denotes in
T13088 6571-6574 DT denotes the
T13089 6596-6602 NN denotes region
T13090 6575-6586 JJ denotes centromeric
T13091 6587-6595 NN denotes flanking
T13092 6603-6604 -LRB- denotes (
T13093 6611-6613 NN denotes S3
T13094 6604-6610 NN denotes Figure
T13095 6613-6614 -RRB- denotes )
T13096 6614-6615 . denotes .
T13097 6615-6787 sentence denotes On average each product was ~750 bp in size, and amplifications were performed using genomic DNA from each of the three affected individuals (family members 6, 7, and 19).
T13098 6616-6618 IN denotes On
T13099 6640-6643 VBD denotes was
T13100 6619-6626 JJ denotes average
T13101 6627-6631 DT denotes each
T13102 6632-6639 NN denotes product
T13103 6644-6645 SYM denotes ~
T13104 6645-6648 CD denotes 750
T13105 6649-6651 NN denotes bp
T13106 6652-6654 IN denotes in
T13107 6655-6659 NN denotes size
T13108 6659-6661 , denotes ,
T13109 6661-6664 CC denotes and
T13110 6665-6679 NNS denotes amplifications
T13111 6685-6694 VBN denotes performed
T13112 6680-6684 VBD denotes were
T13113 6695-6700 VBG denotes using
T13114 6701-6708 JJ denotes genomic
T13115 6709-6712 NN denotes DNA
T13116 6713-6717 IN denotes from
T13117 6718-6722 DT denotes each
T13118 6723-6725 IN denotes of
T13119 6726-6729 DT denotes the
T13120 6745-6756 NNS denotes individuals
T13121 6730-6735 CD denotes three
T13122 6736-6744 VBN denotes affected
T13123 6757-6758 -LRB- denotes (
T13124 6773-6774 CD denotes 6
T13125 6758-6764 NN denotes family
T13126 6765-6772 NNS denotes members
T13127 6774-6776 , denotes ,
T13128 6776-6777 CD denotes 7
T13129 6777-6779 , denotes ,
T13130 6779-6782 CC denotes and
T13131 6783-6785 CD denotes 19
T13132 6785-6786 -RRB- denotes )
T13133 6786-6787 . denotes .
T13134 6787-6981 sentence denotes Dye-terminator sequencing of each product was performed using the forward and reverse primers designed for amplification; running and analysis of each fragment was performed as described above.
T13135 6788-6791 NN denotes Dye
T13136 6792-6802 NN denotes terminator
T13137 6791-6792 HYPH denotes -
T13138 6803-6813 NN denotes sequencing
T13139 6834-6843 VBN denotes performed
T13140 6814-6816 IN denotes of
T13141 6817-6821 DT denotes each
T13142 6822-6829 NN denotes product
T13143 6830-6833 VBD denotes was
T13144 6952-6961 VBN denotes performed
T13145 6844-6849 VBG denotes using
T13146 6850-6853 DT denotes the
T13147 6874-6881 NNS denotes primers
T13148 6854-6861 JJ denotes forward
T13149 6862-6865 CC denotes and
T13150 6866-6873 JJ denotes reverse
T13151 6882-6890 VBN denotes designed
T13152 6891-6894 IN denotes for
T13153 6895-6908 NN denotes amplification
T13154 6908-6909 : denotes ;
T13155 6910-6917 VBG denotes running
T13156 6922-6930 NN denotes analysis
T13157 6918-6921 CC denotes and
T13158 6931-6933 IN denotes of
T13159 6934-6938 DT denotes each
T13160 6939-6947 NN denotes fragment
T13161 6948-6951 VBD denotes was
T13162 6962-6964 IN denotes as
T13163 6965-6974 VBN denotes described
T13164 6975-6980 RB denotes above
T13165 6980-6981 . denotes .
T13166 6981-7434 sentence denotes Amplification of a fragment from a normal diploid genome was denoted by the presence of a heterozygous polymorphism; amplification of a fragment from a region of the genome harboring a heterozygous genomic deletion was inferred when homozygosity for the major allele and the minor allele were noted among the three affected family members (i.e., this is inconsistent with Mendelian inheritance in related individuals known to share a common haplotype).
T13167 6982-6995 NN denotes Amplification
T13168 7043-7050 VBN denotes denoted
T13169 6996-6998 IN denotes of
T13170 6999-7000 DT denotes a
T13171 7001-7009 NN denotes fragment
T13172 7010-7014 IN denotes from
T13173 7015-7016 DT denotes a
T13174 7032-7038 NN denotes genome
T13175 7017-7023 JJ denotes normal
T13176 7024-7031 JJ denotes diploid
T13177 7039-7042 VBD denotes was
T13178 7201-7209 VBN denotes inferred
T13179 7051-7053 IN denotes by
T13180 7054-7057 DT denotes the
T13181 7058-7066 NN denotes presence
T13182 7067-7069 IN denotes of
T13183 7070-7071 DT denotes a
T13184 7085-7097 NN denotes polymorphism
T13185 7072-7084 JJ denotes heterozygous
T13186 7097-7098 : denotes ;
T13187 7099-7112 NN denotes amplification
T13188 7113-7115 IN denotes of
T13189 7116-7117 DT denotes a
T13190 7118-7126 NN denotes fragment
T13191 7127-7131 IN denotes from
T13192 7132-7133 DT denotes a
T13193 7134-7140 NN denotes region
T13194 7141-7143 IN denotes of
T13195 7144-7147 DT denotes the
T13196 7148-7154 NN denotes genome
T13197 7155-7164 VBG denotes harboring
T13198 7165-7166 DT denotes a
T13199 7188-7196 NN denotes deletion
T13200 7167-7179 JJ denotes heterozygous
T13201 7180-7187 JJ denotes genomic
T13202 7197-7200 VBD denotes was
T13203 7210-7214 WRB denotes when
T13204 7275-7280 VBN denotes noted
T13205 7215-7227 NN denotes homozygosity
T13206 7228-7231 IN denotes for
T13207 7232-7235 DT denotes the
T13208 7242-7248 NN denotes allele
T13209 7236-7241 JJ denotes major
T13210 7249-7252 CC denotes and
T13211 7253-7256 DT denotes the
T13212 7263-7269 NN denotes allele
T13213 7257-7262 JJ denotes minor
T13214 7270-7274 VBD denotes were
T13215 7281-7286 IN denotes among
T13216 7287-7290 DT denotes the
T13217 7313-7320 NNS denotes members
T13218 7291-7296 CD denotes three
T13219 7297-7305 VBN denotes affected
T13220 7306-7312 NN denotes family
T13221 7321-7322 -LRB- denotes (
T13222 7333-7335 VBZ denotes is
T13223 7322-7326 FW denotes i.e.
T13224 7326-7328 , denotes ,
T13225 7328-7332 DT denotes this
T13226 7336-7348 JJ denotes inconsistent
T13227 7349-7353 IN denotes with
T13228 7354-7363 JJ denotes Mendelian
T13229 7364-7375 NN denotes inheritance
T13230 7376-7378 IN denotes in
T13231 7379-7386 JJ denotes related
T13232 7387-7398 NNS denotes individuals
T13233 7399-7404 VBN denotes known
T13234 7405-7407 TO denotes to
T13235 7408-7413 VB denotes share
T13236 7414-7415 DT denotes a
T13237 7423-7432 NN denotes haplotype
T13238 7416-7422 JJ denotes common
T13239 7432-7433 -RRB- denotes )
T13240 7433-7434 . denotes .
T13241 7434-7623 sentence denotes Using the data from the experiments described above we were able to limit the size of unknown regions flanking the deletion to ~4 kb on the telomeric side and 7 kb on the centromeric side.
T13242 7435-7440 VBG denotes Using
T13243 7490-7494 VBD denotes were
T13244 7441-7444 DT denotes the
T13245 7445-7449 NNS denotes data
T13246 7450-7454 IN denotes from
T13247 7455-7458 DT denotes the
T13248 7459-7470 NNS denotes experiments
T13249 7471-7480 VBN denotes described
T13250 7481-7486 RB denotes above
T13251 7487-7489 PRP denotes we
T13252 7495-7499 JJ denotes able
T13253 7500-7502 TO denotes to
T13254 7503-7508 VB denotes limit
T13255 7509-7512 DT denotes the
T13256 7513-7517 NN denotes size
T13257 7518-7520 IN denotes of
T13258 7521-7528 JJ denotes unknown
T13259 7529-7536 NNS denotes regions
T13260 7537-7545 VBG denotes flanking
T13261 7546-7549 DT denotes the
T13262 7550-7558 NN denotes deletion
T13263 7559-7561 IN denotes to
T13264 7562-7563 SYM denotes ~
T13265 7563-7564 CD denotes 4
T13266 7565-7567 NN denotes kb
T13267 7568-7570 IN denotes on
T13268 7571-7574 DT denotes the
T13269 7585-7589 NN denotes side
T13270 7575-7584 JJ denotes telomeric
T13271 7590-7593 CC denotes and
T13272 7594-7595 CD denotes 7
T13273 7596-7598 NN denotes kb
T13274 7599-7601 IN denotes on
T13275 7602-7605 DT denotes the
T13276 7618-7622 NN denotes side
T13277 7606-7617 JJ denotes centromeric
T13278 7622-7623 . denotes .
T13279 7623-7967 sentence denotes All combinations of forward primers from the newly defined region flanking the deletion on the telomeric side with reverse primers from the newly defined region flanking the deletion on the centromeric side were used in PCR amplification reactions performed with DNA from the three affected family members and single unaffected family members.
T13280 7624-7627 DT denotes All
T13281 7628-7640 NNS denotes combinations
T13282 7836-7840 VBN denotes used
T13283 7641-7643 IN denotes of
T13284 7644-7651 JJ denotes forward
T13285 7652-7659 NNS denotes primers
T13286 7660-7664 IN denotes from
T13287 7665-7668 DT denotes the
T13288 7683-7689 NN denotes region
T13289 7669-7674 RB denotes newly
T13290 7675-7682 VBN denotes defined
T13291 7690-7698 VBG denotes flanking
T13292 7699-7702 DT denotes the
T13293 7703-7711 NN denotes deletion
T13294 7712-7714 IN denotes on
T13295 7715-7718 DT denotes the
T13296 7729-7733 NN denotes side
T13297 7719-7728 JJ denotes telomeric
T13298 7734-7738 IN denotes with
T13299 7739-7746 JJ denotes reverse
T13300 7747-7754 NNS denotes primers
T13301 7755-7759 IN denotes from
T13302 7760-7763 DT denotes the
T13303 7778-7784 NN denotes region
T13304 7764-7769 RB denotes newly
T13305 7770-7777 VBN denotes defined
T13306 7785-7793 VBG denotes flanking
T13307 7794-7797 DT denotes the
T13308 7798-7806 NN denotes deletion
T13309 7807-7809 IN denotes on
T13310 7810-7813 DT denotes the
T13311 7826-7830 NN denotes side
T13312 7814-7825 JJ denotes centromeric
T13313 7831-7835 VBD denotes were
T13314 7841-7843 IN denotes in
T13315 7844-7847 NN denotes PCR
T13316 7848-7861 NN denotes amplification
T13317 7862-7871 NNS denotes reactions
T13318 7872-7881 VBN denotes performed
T13319 7882-7886 IN denotes with
T13320 7887-7890 NN denotes DNA
T13321 7891-7895 IN denotes from
T13322 7896-7899 DT denotes the
T13323 7922-7929 NNS denotes members
T13324 7900-7905 CD denotes three
T13325 7906-7914 VBN denotes affected
T13326 7915-7921 NN denotes family
T13327 7930-7933 CC denotes and
T13328 7934-7940 JJ denotes single
T13329 7959-7966 NNS denotes members
T13330 7941-7951 JJ denotes unaffected
T13331 7952-7958 NN denotes family
T13332 7966-7967 . denotes .
T13333 7967-8083 sentence denotes This experiment was performed in an attempt to amplify across the deleted fragment and define the exact breakpoint.
T13334 7968-7972 DT denotes This
T13335 7973-7983 NN denotes experiment
T13336 7988-7997 VBN denotes performed
T13337 7984-7987 VBD denotes was
T13338 7998-8000 IN denotes in
T13339 8001-8003 DT denotes an
T13340 8004-8011 NN denotes attempt
T13341 8012-8014 TO denotes to
T13342 8015-8022 VB denotes amplify
T13343 8023-8029 IN denotes across
T13344 8030-8033 DT denotes the
T13345 8042-8050 NN denotes fragment
T13346 8034-8041 VBN denotes deleted
T13347 8051-8054 CC denotes and
T13348 8055-8061 VB denotes define
T13349 8062-8065 DT denotes the
T13350 8072-8082 NN denotes breakpoint
T13351 8066-8071 JJ denotes exact
T13352 8082-8083 . denotes .
T13353 8083-8291 sentence denotes A single fragment was obtained from the third forward primer from the telomeric side (T3f 5′-TGAATGCTCAATTTTCCAGC-3′) with the 11th reverse primer from the centromeric side (C11r 5′-GGGAAAATGGATAGAGGGTG-3′).
T13354 8084-8085 DT denotes A
T13355 8093-8101 NN denotes fragment
T13356 8086-8092 JJ denotes single
T13357 8106-8114 VBN denotes obtained
T13358 8102-8105 VBD denotes was
T13359 8115-8119 IN denotes from
T13360 8120-8123 DT denotes the
T13361 8138-8144 NN denotes primer
T13362 8124-8129 JJ denotes third
T13363 8130-8137 JJ denotes forward
T13364 8145-8149 IN denotes from
T13365 8150-8153 DT denotes the
T13366 8164-8168 NN denotes side
T13367 8154-8163 JJ denotes telomeric
T13368 8169-8170 -LRB- denotes (
T13369 8177-8197 NN denotes TGAATGCTCAATTTTCCAGC
T13370 8170-8173 NN denotes T3f
T13371 8174-8175 CD denotes 5
T13372 8175-8176 SYM denotes ′
T13373 8176-8177 HYPH denotes -
T13374 8197-8198 HYPH denotes -
T13375 8198-8199 CD denotes 3
T13376 8199-8200 SYM denotes ′
T13377 8200-8201 -RRB- denotes )
T13378 8202-8206 IN denotes with
T13379 8207-8210 DT denotes the
T13380 8224-8230 NN denotes primer
T13381 8211-8215 JJ denotes 11th
T13382 8216-8223 JJ denotes reverse
T13383 8231-8235 IN denotes from
T13384 8236-8239 DT denotes the
T13385 8252-8256 NN denotes side
T13386 8240-8251 JJ denotes centromeric
T13387 8257-8258 -LRB- denotes (
T13388 8266-8286 NN denotes GGGAAAATGGATAGAGGGTG
T13389 8258-8262 NN denotes C11r
T13390 8263-8264 CD denotes 5
T13391 8264-8265 SYM denotes ′
T13392 8265-8266 HYPH denotes -
T13393 8286-8287 HYPH denotes -
T13394 8287-8288 CD denotes 3
T13395 8288-8289 SYM denotes ′
T13396 8289-8290 -RRB- denotes )
T13397 8290-8291 . denotes .
T13398 8291-8418 sentence denotes The fragment, which is 953 bp in size, was sequenced as described above and compared to the current build of the human genome.
T13399 8292-8295 DT denotes The
T13400 8296-8304 NN denotes fragment
T13401 8335-8344 VBN denotes sequenced
T13402 8304-8306 , denotes ,
T13403 8306-8311 WDT denotes which
T13404 8312-8314 VBZ denotes is
T13405 8315-8318 CD denotes 953
T13406 8319-8321 NN denotes bp
T13407 8322-8324 IN denotes in
T13408 8325-8329 NN denotes size
T13409 8329-8331 , denotes ,
T13410 8331-8334 VBD denotes was
T13411 8345-8347 IN denotes as
T13412 8348-8357 VBN denotes described
T13413 8358-8363 RB denotes above
T13414 8364-8367 CC denotes and
T13415 8368-8376 VBN denotes compared
T13416 8377-8379 IN denotes to
T13417 8380-8383 DT denotes the
T13418 8392-8397 NN denotes build
T13419 8384-8391 JJ denotes current
T13420 8398-8400 IN denotes of
T13421 8401-8404 DT denotes the
T13422 8411-8417 NN denotes genome
T13423 8405-8410 JJ denotes human
T13424 8417-8418 . denotes .
T13425 8418-8813 sentence denotes A similar series of experiments was performed to identify the deletion breakpoints in families H27 and H33; we were able to amplify a 369-bp PCR product across the breakpoint found in affected members of family H27 using primer pair H27-11F 5′-GACCTCAAGAAGGCATGAATAC-3′ and H27-3R 5′-ATGGTGGCCAGGTACACAAG-3′ (Figure S4), but to date we have been unable to identify the breakpoint in family H33.
T13426 8419-8420 DT denotes A
T13427 8429-8435 NN denotes series
T13428 8421-8428 JJ denotes similar
T13429 8455-8464 VBN denotes performed
T13430 8436-8438 IN denotes of
T13431 8439-8450 NNS denotes experiments
T13432 8451-8454 VBD denotes was
T13433 8530-8534 VBD denotes were
T13434 8465-8467 TO denotes to
T13435 8468-8476 VB denotes identify
T13436 8477-8480 DT denotes the
T13437 8490-8501 NNS denotes breakpoints
T13438 8481-8489 NN denotes deletion
T13439 8502-8504 IN denotes in
T13440 8505-8513 NNS denotes families
T13441 8514-8517 NN denotes H27
T13442 8518-8521 CC denotes and
T13443 8522-8525 NN denotes H33
T13444 8525-8526 : denotes ;
T13445 8527-8529 PRP denotes we
T13446 8535-8539 JJ denotes able
T13447 8540-8542 TO denotes to
T13448 8543-8550 VB denotes amplify
T13449 8551-8552 DT denotes a
T13450 8564-8571 NN denotes product
T13451 8553-8556 CD denotes 369
T13452 8557-8559 NN denotes bp
T13453 8556-8557 HYPH denotes -
T13454 8560-8563 NN denotes PCR
T13455 8572-8578 IN denotes across
T13456 8579-8582 DT denotes the
T13457 8583-8593 NN denotes breakpoint
T13458 8594-8599 VBN denotes found
T13459 8600-8602 IN denotes in
T13460 8603-8611 VBN denotes affected
T13461 8612-8619 NNS denotes members
T13462 8620-8622 IN denotes of
T13463 8623-8629 NN denotes family
T13464 8630-8633 NN denotes H27
T13465 8634-8639 VBG denotes using
T13466 8640-8646 NN denotes primer
T13467 8647-8651 NN denotes pair
T13468 8652-8655 NN denotes H27
T13469 8656-8659 NN denotes 11F
T13470 8655-8656 HYPH denotes -
T13471 8663-8685 NN denotes GACCTCAAGAAGGCATGAATAC
T13472 8660-8661 CD denotes 5
T13473 8661-8662 SYM denotes ′
T13474 8662-8663 HYPH denotes -
T13475 8685-8686 HYPH denotes -
T13476 8686-8687 CD denotes 3
T13477 8687-8688 SYM denotes ′
T13478 8689-8692 CC denotes and
T13479 8693-8696 NN denotes H27
T13480 8697-8699 NN denotes 3R
T13481 8696-8697 HYPH denotes -
T13482 8703-8723 NN denotes ATGGTGGCCAGGTACACAAG
T13483 8700-8701 CD denotes 5
T13484 8701-8702 SYM denotes ′
T13485 8702-8703 HYPH denotes -
T13486 8723-8724 HYPH denotes -
T13487 8724-8725 CD denotes 3
T13488 8725-8726 SYM denotes ′
T13489 8727-8728 -LRB- denotes (
T13490 8735-8737 NN denotes S4
T13491 8728-8734 NN denotes Figure
T13492 8737-8738 -RRB- denotes )
T13493 8738-8740 , denotes ,
T13494 8740-8743 CC denotes but
T13495 8744-8746 IN denotes to
T13496 8760-8764 VBN denotes been
T13497 8747-8751 NN denotes date
T13498 8752-8754 PRP denotes we
T13499 8755-8759 VBP denotes have
T13500 8765-8771 JJ denotes unable
T13501 8772-8774 TO denotes to
T13502 8775-8783 VB denotes identify
T13503 8784-8787 DT denotes the
T13504 8788-8798 NN denotes breakpoint
T13505 8799-8801 IN denotes in
T13506 8802-8808 NN denotes family
T13507 8809-8812 NN denotes H33
T13508 8812-8813 . denotes .
T13807 8815-8822 NNP denotes Western
T13808 8823-8827 NN denotes blot
T13809 8828-8836 NN denotes analysis
T13810 8837-8839 IN denotes in
T13811 8840-8845 NN denotes SCA15
T13812 8846-8854 NNS denotes patients
T13813 8854-8855 . denotes .
T13814 8855-9085 sentence denotes EBV immortalized lymphoblasts from three affected family members who carry the deletion and one family member without the mutation were used as a readily accessible source of protein; all samples came from members of family AUS1.
T13815 8856-8859 NN denotes EBV
T13816 8873-8885 NNS denotes lymphoblasts
T13817 8860-8872 VBN denotes immortalized
T13818 8992-8996 VBN denotes used
T13819 8886-8890 IN denotes from
T13820 8891-8896 CD denotes three
T13821 8913-8920 NNS denotes members
T13822 8897-8905 VBN denotes affected
T13823 8906-8912 NN denotes family
T13824 8921-8924 WP denotes who
T13825 8925-8930 VBP denotes carry
T13826 8931-8934 DT denotes the
T13827 8935-8943 NN denotes deletion
T13828 8944-8947 CC denotes and
T13829 8948-8951 CD denotes one
T13830 8959-8965 NN denotes member
T13831 8952-8958 NN denotes family
T13832 8966-8973 IN denotes without
T13833 8974-8977 DT denotes the
T13834 8978-8986 NN denotes mutation
T13835 8987-8991 VBD denotes were
T13836 9052-9056 VBD denotes came
T13837 8997-8999 IN denotes as
T13838 9000-9001 DT denotes a
T13839 9021-9027 NN denotes source
T13840 9002-9009 RB denotes readily
T13841 9010-9020 JJ denotes accessible
T13842 9028-9030 IN denotes of
T13843 9031-9038 NN denotes protein
T13844 9038-9039 : denotes ;
T13845 9040-9043 DT denotes all
T13846 9044-9051 NNS denotes samples
T13847 9057-9061 IN denotes from
T13848 9062-9069 NNS denotes members
T13849 9070-9072 IN denotes of
T13850 9073-9079 NN denotes family
T13851 9080-9084 NN denotes AUS1
T13852 9084-9085 . denotes .
T13853 9085-9251 sentence denotes Protein extraction was performed using lysis buffer containing 1× TBS, 1% Triton X-100, and a cocktail of protease inhibitors (Roche) with overnight lysis at −80 °C.
T13854 9086-9093 NN denotes Protein
T13855 9094-9104 NN denotes extraction
T13856 9109-9118 VBN denotes performed
T13857 9105-9108 VBD denotes was
T13858 9119-9124 VBG denotes using
T13859 9125-9130 NN denotes lysis
T13860 9131-9137 NN denotes buffer
T13861 9138-9148 VBG denotes containing
T13862 9149-9150 CD denotes 1
T13863 9152-9155 NN denotes TBS
T13864 9150-9151 SYM denotes ×
T13865 9155-9157 , denotes ,
T13866 9157-9158 CD denotes 1
T13867 9158-9159 NN denotes %
T13868 9167-9168 NN denotes X
T13869 9160-9166 NN denotes Triton
T13870 9168-9169 HYPH denotes -
T13871 9169-9172 CD denotes 100
T13872 9172-9174 , denotes ,
T13873 9174-9177 CC denotes and
T13874 9178-9179 DT denotes a
T13875 9180-9188 NN denotes cocktail
T13876 9189-9191 IN denotes of
T13877 9192-9200 NN denotes protease
T13878 9201-9211 NNS denotes inhibitors
T13879 9212-9213 -LRB- denotes (
T13880 9213-9218 NNP denotes Roche
T13881 9218-9219 -RRB- denotes )
T13882 9220-9224 IN denotes with
T13883 9225-9234 JJ denotes overnight
T13884 9235-9240 NN denotes lysis
T13885 9241-9243 IN denotes at
T13886 9244-9245 SYM denotes −
T13887 9245-9247 CD denotes 80
T13888 9248-9250 NN denotes °C
T13889 9250-9251 . denotes .
T13890 9251-9428 sentence denotes Homogenates were diluted appropriately, mixed with 4× reducing sample buffer, and loaded onto 4%–12% precast gradient gels (NuPAGE, Invitrogen) for SDS-PAGE and immunoblotting.
T13891 9252-9263 NNS denotes Homogenates
T13892 9269-9276 VBN denotes diluted
T13893 9264-9268 VBD denotes were
T13894 9277-9290 RB denotes appropriately
T13895 9290-9292 , denotes ,
T13896 9292-9297 VBN denotes mixed
T13897 9298-9302 IN denotes with
T13898 9303-9304 CD denotes 4
T13899 9322-9328 NN denotes buffer
T13900 9304-9305 SYM denotes ×
T13901 9306-9314 VBG denotes reducing
T13902 9315-9321 NN denotes sample
T13903 9328-9330 , denotes ,
T13904 9330-9333 CC denotes and
T13905 9334-9340 VBN denotes loaded
T13906 9341-9345 IN denotes onto
T13907 9346-9347 CD denotes 4
T13908 9347-9348 NN denotes %
T13909 9370-9374 NNS denotes gels
T13910 9348-9349 SYM denotes –
T13911 9351-9352 NN denotes %
T13912 9349-9351 CD denotes 12
T13913 9353-9360 JJ denotes precast
T13914 9361-9369 NN denotes gradient
T13915 9375-9376 -LRB- denotes (
T13916 9376-9382 NN denotes NuPAGE
T13917 9382-9384 , denotes ,
T13918 9384-9394 NNP denotes Invitrogen
T13919 9394-9395 -RRB- denotes )
T13920 9396-9399 IN denotes for
T13921 9400-9403 NN denotes SDS
T13922 9404-9408 NN denotes PAGE
T13923 9403-9404 HYPH denotes -
T13924 9409-9412 CC denotes and
T13925 9413-9427 NN denotes immunoblotting
T13926 9427-9428 . denotes .
T13927 9428-9524 sentence denotes The antibodies to ITPR1 (1:1,000) and ACTB (1:5,000) were used as recommended by manufacturers.
T13928 9429-9432 DT denotes The
T13929 9433-9443 NNS denotes antibodies
T13930 9487-9491 VBN denotes used
T13931 9444-9446 IN denotes to
T13932 9447-9452 NN denotes ITPR1
T13933 9453-9454 -LRB- denotes (
T13934 9454-9455 CD denotes 1
T13935 9455-9456 SYM denotes :
T13936 9456-9461 CD denotes 1,000
T13937 9461-9462 -RRB- denotes )
T13938 9463-9466 CC denotes and
T13939 9467-9471 NN denotes ACTB
T13940 9472-9473 -LRB- denotes (
T13941 9473-9474 CD denotes 1
T13942 9474-9475 SYM denotes :
T13943 9475-9480 CD denotes 5,000
T13944 9480-9481 -RRB- denotes )
T13945 9482-9486 VBD denotes were
T13946 9492-9494 IN denotes as
T13947 9495-9506 VBN denotes recommended
T13948 9507-9509 IN denotes by
T13949 9510-9523 NNS denotes manufacturers
T13950 9523-9524 . denotes .
R2535 T8971 T8972 npadvmod Genome,wide
R2536 T8972 T8974 amod wide,linkage
R2537 T8973 T8972 punct -,wide
R2538 T8975 T8974 prep in,linkage
R2539 T8976 T8975 pobj mice,in
R2540 T8977 T8974 punct .,linkage
R2541 T8979 T8980 compound One,hundred
R2542 T8980 T8981 nummod hundred,fragments
R2543 T8981 T8985 nsubjpass fragments,amplified
R2544 T8982 T8980 cc and,hundred
R2545 T8983 T8980 conj twenty,hundred
R2546 T8984 T8981 compound DNA,fragments
R2547 T8986 T8985 auxpass were,amplified
R2548 T8987 T8985 prep across,amplified
R2549 T8988 T8989 det the,genome
R2550 T8989 T8987 pobj genome,across
R2551 T8990 T8985 punct ", ",amplified
R2552 T8991 T8985 npadvmod each,amplified
R2553 T8992 T8991 acl selected,each
R2554 T8993 T8994 aux to,contain
R2555 T8994 T8992 advcl contain,selected
R2556 T8995 T8996 nummod one,SNPs
R2557 T8996 T8994 dobj SNPs,contain
R2558 T8997 T8995 cc or,one
R2559 T8998 T8995 conj more,one
R2560 T8999 T9000 npadvmod strain,specific
R2561 T9000 T8996 amod specific,SNPs
R2562 T9001 T9000 punct -,specific
R2563 T9002 T9003 dep that,differentiate
R2564 T9003 T8996 relcl differentiate,SNPs
R2565 T9004 T9003 aux would,differentiate
R2566 T9005 T9003 prep between,differentiate
R2567 T9006 T9007 nmod C57BL,6J
R2568 T9007 T9009 nmod 6J,strains
R2569 T9008 T9007 punct /,6J
R2570 T9009 T9005 pobj strains,between
R2571 T9010 T9007 cc and,6J
R2572 T9011 T9012 compound 129x1,SvJ
R2573 T9012 T9007 conj SvJ,6J
R2574 T9013 T9012 punct /,SvJ
R2575 T9014 T9009 amod inbred,strains
R2576 T9015 T9016 punct [,12
R2577 T9016 T8985 parataxis 12,amplified
R2578 T9017 T9016 punct ],12
R2579 T9018 T8985 punct .,amplified
R2580 T9020 T9021 det Each,fragment
R2581 T9021 T9022 nsubjpass fragment,amplified
R2582 T9022 T9025 ccomp amplified,performed
R2583 T9023 T9022 auxpass was,amplified
R2584 T9024 T9022 advmod initially,amplified
R2585 T9026 T9022 prep in,amplified
R2586 T9027 T9028 nummod 11,mice
R2587 T9028 T9026 pobj mice,in
R2588 T9029 T9028 amod affected,mice
R2589 T9030 T9028 cc and,mice
R2590 T9031 T9032 nummod nine,mice
R2591 T9032 T9028 conj mice,mice
R2592 T9033 T9032 amod unaffected,mice
R2593 T9034 T9025 punct ;,performed
R2594 T9035 T9036 compound genotype,calling
R2595 T9036 T9025 nsubjpass calling,performed
R2596 T9037 T9025 auxpass was,performed
R2597 T9038 T9025 prep by,performed
R2598 T9039 T9040 compound dye,terminator
R2599 T9040 T9042 compound terminator,sequencing
R2600 T9041 T9040 punct -,terminator
R2601 T9042 T9038 pobj sequencing,by
R2602 T9043 T9042 prep of,sequencing
R2603 T9044 T9045 det these,fragments
R2604 T9045 T9043 pobj fragments,of
R2605 T9046 T9025 punct .,performed
R2606 T9048 T9049 compound Linkage,analysis
R2607 T9049 T9050 nsubjpass analysis,performed
R2608 T9051 T9049 acl using,analysis
R2609 T9052 T9053 det these,data
R2610 T9053 T9051 dobj data,using
R2611 T9054 T9050 auxpass was,performed
R2612 T9055 T9050 advcl using,performed
R2613 T9056 T9055 dobj mlink,using
R2614 T9057 T9058 punct [,13
R2615 T9058 T9055 parataxis 13,using
R2616 T9059 T9058 punct ],13
R2617 T9060 T9050 punct ", ",performed
R2618 T9061 T9062 dep which,revealed
R2619 T9062 T9050 advcl revealed,performed
R2620 T9063 T9064 det a,linkage
R2621 T9064 T9062 dobj linkage,revealed
R2622 T9065 T9064 amod positive,linkage
R2623 T9066 T9062 prep at,revealed
R2624 T9067 T9068 compound Chromosome,6qE1
R2625 T9068 T9066 pobj 6qE1,at
R2626 T9069 T9062 punct ", ",revealed
R2627 T9070 T9062 prep on,revealed
R2628 T9071 T9072 det the,background
R2629 T9072 T9070 pobj background,on
R2630 T9073 T9074 compound 129x1,SvJ
R2631 T9074 T9072 compound SvJ,background
R2632 T9075 T9074 punct /,SvJ
R2633 T9076 T9077 punct (,score
R2634 T9077 T9072 parataxis score,background
R2635 T9078 T9079 nummod two,point
R2636 T9079 T9077 compound point,score
R2637 T9080 T9079 punct -,point
R2638 T9081 T9077 compound LOD,score
R2639 T9082 T9077 nummod 5.13,score
R2640 T9083 T9077 prep at,score
R2641 T9084 T9085 compound marker,20.MMHAP85FLG2
R2642 T9085 T9083 pobj 20.MMHAP85FLG2,at
R2643 T9086 T9077 punct ),score
R2644 T9087 T9050 punct .,performed
R2645 T9089 T9090 prep In,performed
R2646 T9091 T9092 det an,attempt
R2647 T9092 T9089 pobj attempt,In
R2648 T9093 T9094 aux to,narrow
R2649 T9094 T9092 acl narrow,attempt
R2650 T9095 T9096 det the,interval
R2651 T9096 T9094 dobj interval,narrow
R2652 T9097 T9096 compound disease,interval
R2653 T9098 T9090 nsubj we,performed
R2654 T9099 T9100 compound backcross,experiments
R2655 T9100 T9090 dobj experiments,performed
R2656 T9101 T9102 dep that,resulted
R2657 T9102 T9100 relcl resulted,experiments
R2658 T9103 T9102 prep in,resulted
R2659 T9104 T9105 det the,generation
R2660 T9105 T9103 pobj generation,in
R2661 T9106 T9105 prep of,generation
R2662 T9107 T9108 nummod three,mice
R2663 T9108 T9106 pobj mice,of
R2664 T9109 T9108 amod additional,mice
R2665 T9110 T9108 amod affected,mice
R2666 T9111 T9090 punct .,performed
R2667 T9113 T9114 nsubj Genotyping,revealed
R2668 T9115 T9113 prep of,Genotyping
R2669 T9116 T9117 det all,mice
R2670 T9117 T9115 pobj mice,of
R2671 T9118 T9117 amod affected,mice
R2672 T9119 T9113 prep across,Genotyping
R2673 T9120 T9121 det the,interval
R2674 T9121 T9119 pobj interval,across
R2675 T9122 T9123 npadvmod disease,segregating
R2676 T9123 T9121 amod segregating,interval
R2677 T9124 T9123 punct -,segregating
R2678 T9125 T9126 amod flanking,recombinants
R2679 T9126 T9114 dobj recombinants,revealed
R2680 T9127 T9126 cc and,recombinants
R2681 T9128 T9129 det a,region
R2682 T9129 T9126 conj region,recombinants
R2683 T9130 T9129 compound candidate,region
R2684 T9131 T9129 prep of,region
R2685 T9132 T9133 punct ~,5
R2686 T9133 T9134 nummod 5,Mb
R2687 T9134 T9131 pobj Mb,of
R2688 T9135 T9114 punct ", ",revealed
R2689 T9136 T9114 prep between,revealed
R2690 T9137 T9138 compound markers,D6Mit37
R2691 T9138 T9136 pobj D6Mit37,between
R2692 T9139 T9138 cc and,D6Mit37
R2693 T9140 T9138 conj 44.MMHAP85FLG5,D6Mit37
R2694 T9141 T9142 punct (,S1
R2695 T9142 T9114 parataxis S1,revealed
R2696 T9143 T9142 compound Figure,S1
R2697 T9144 T9142 punct ),S1
R2698 T9145 T9114 punct .,revealed
R2699 T9147 T9148 det This,region
R2700 T9148 T9149 nsubj region,contains
R2701 T9150 T9151 nummod 16,genes
R2702 T9151 T9149 dobj genes,contains
R2703 T9152 T9151 cc and,genes
R2704 T9153 T9154 amod predicted,transcripts
R2705 T9154 T9151 conj transcripts,genes
R2706 T9155 T9149 punct .,contains
R2709 T9603 T9602 prep of,Identification
R2710 T9604 T9605 det the,lesion
R2711 T9605 T9603 pobj lesion,of
R2712 T9606 T9605 amod underlying,lesion
R2713 T9607 T9605 amod genetic,lesion
R2714 T9608 T9602 prep in,Identification
R2715 T9609 T9608 pobj mice,in
R2716 T9610 T9602 punct .,Identification
R2717 T9612 T9613 nsubjpass Identification,performed
R2718 T9614 T9612 prep of,Identification
R2719 T9615 T9616 amod similar,phenotypes
R2720 T9616 T9614 pobj phenotypes,of
R2721 T9617 T9616 prep in,phenotypes
R2722 T9618 T9617 pobj mice,in
R2723 T9619 T9618 acl linked,mice
R2724 T9620 T9619 prep to,linked
R2725 T9621 T9622 det the,interval
R2726 T9622 T9620 pobj interval,to
R2727 T9623 T9622 compound 6qE1,interval
R2728 T9624 T9613 auxpass was,performed
R2729 T9625 T9613 prep by,performed
R2730 T9626 T9627 compound literature,searches
R2731 T9627 T9625 pobj searches,by
R2732 T9628 T9613 punct .,performed
R2733 T9630 T9631 nsubj This,revealed
R2734 T9632 T9633 det the,mouse
R2735 T9633 T9631 dobj mouse,revealed
R2736 T9634 T9635 compound Itpr1opt,opt
R2737 T9635 T9633 compound opt,mouse
R2738 T9636 T9635 punct /,opt
R2739 T9637 T9633 punct ", ",mouse
R2740 T9638 T9639 prep in,caused
R2741 T9639 T9633 relcl caused,mouse
R2742 T9640 T9638 pobj which,in
R2743 T9641 T9639 nsubjpass disease,caused
R2744 T9642 T9639 auxpass is,caused
R2745 T9643 T9639 agent by,caused
R2746 T9644 T9645 amod homozygous,mutation
R2747 T9645 T9643 pobj mutation,by
R2748 T9646 T9645 compound deletion,mutation
R2749 T9647 T9645 prep of,mutation
R2750 T9648 T9649 nmod exons,43
R2751 T9649 T9647 pobj 43,of
R2752 T9650 T9649 cc and,43
R2753 T9651 T9649 conj 44,43
R2754 T9652 T9649 prep of,43
R2755 T9653 T9652 pobj Itpr1,of
R2756 T9654 T9631 punct .,revealed
R2757 T9656 T9657 compound Primer,pairs
R2758 T9657 T9658 nsubjpass pairs,designed
R2759 T9659 T9658 auxpass were,designed
R2760 T9660 T9661 aux to,sequence
R2761 T9661 T9658 advcl sequence,designed
R2762 T9662 T9661 dobj each,sequence
R2763 T9663 T9662 prep of,each
R2764 T9664 T9665 det the,exons
R2765 T9665 T9663 pobj exons,of
R2766 T9666 T9665 compound coding,exons
R2767 T9667 T9662 cc and,each
R2768 T9668 T9669 advmod at,50
R2769 T9669 T9671 nummod 50,bp
R2770 T9670 T9669 advmod least,50
R2771 T9671 T9662 conj bp,each
R2772 T9672 T9671 prep of,bp
R2773 T9673 T9674 det each,sequence
R2774 T9674 T9672 pobj sequence,of
R2775 T9675 T9674 amod flanking,sequence
R2776 T9676 T9674 amod intronic,sequence
R2777 T9677 T9662 prep of,each
R2778 T9678 T9677 pobj Itpr1,of
R2779 T9679 T9658 punct .,designed
R2780 T9681 T9682 compound PCR,amplification
R2781 T9682 T9683 nsubjpass amplification,performed
R2782 T9684 T9682 prep of,amplification
R2783 T9685 T9686 det each,exon
R2784 T9686 T9684 pobj exon,of
R2785 T9687 T9683 auxpass was,performed
R2786 T9688 T9683 advcl using,performed
R2787 T9689 T9688 dobj DNA,using
R2788 T9690 T9689 prep from,DNA
R2789 T9691 T9692 nummod two,mice
R2790 T9692 T9690 pobj mice,from
R2791 T9693 T9692 amod affected,mice
R2792 T9694 T9688 prep as,using
R2793 T9695 T9694 pobj templates,as
R2794 T9696 T9683 punct .,performed
R2795 T9698 T9699 det The,mutation
R2796 T9699 T9703 nsubjpass mutation,confirmed
R2797 T9700 T9701 compound Itpr1Δ18,Δ18
R2798 T9701 T9699 compound Δ18,mutation
R2799 T9702 T9701 punct /,Δ18
R2800 T9704 T9703 auxpass was,confirmed
R2801 T9705 T9703 prep by,confirmed
R2802 T9706 T9705 pobj sequencing,by
R2803 T9707 T9703 prep in,confirmed
R2804 T9708 T9709 det all,mice
R2805 T9709 T9707 pobj mice,in
R2806 T9710 T9709 amod affected,mice
R2807 T9711 T9712 punct (,S2
R2808 T9712 T9703 parataxis S2,confirmed
R2809 T9713 T9712 compound Figure,S2
R2810 T9714 T9712 punct ),S2
R2811 T9715 T9703 punct .,confirmed
R2812 T9717 T9718 amod Breeding,experiments
R2813 T9718 T9719 nsubjpass experiments,performed
R2814 T9720 T9719 auxpass were,performed
R2815 T9721 T9719 prep between,performed
R2816 T9722 T9723 nummod two,mice
R2817 T9723 T9721 pobj mice,between
R2818 T9724 T9723 amod female,mice
R2819 T9725 T9723 amod heterozygous,mice
R2820 T9726 T9725 prep for,heterozygous
R2821 T9727 T9728 det the,mutation
R2822 T9728 T9726 pobj mutation,for
R2823 T9729 T9728 amod current,mutation
R2824 T9730 T9731 punct (,Δ18
R2825 T9731 T9728 parataxis Δ18,mutation
R2826 T9732 T9731 compound Itpr1wt,Δ18
R2827 T9733 T9731 punct /,Δ18
R2828 T9734 T9731 punct ),Δ18
R2829 T9735 T9723 cc and,mice
R2830 T9736 T9737 det a,mouse
R2831 T9737 T9723 conj mouse,mice
R2832 T9738 T9737 amod male,mouse
R2833 T9739 T9737 amod heterozygous,mouse
R2834 T9740 T9739 prep for,heterozygous
R2835 T9741 T9742 det the,mutation
R2836 T9742 T9740 pobj mutation,for
R2837 T9743 T9742 compound Itpr1opt,mutation
R2838 T9744 T9745 punct (,opt
R2839 T9745 T9742 parataxis opt,mutation
R2840 T9746 T9745 compound Itpr1wt,opt
R2841 T9747 T9745 punct /,opt
R2842 T9748 T9745 punct ),opt
R2843 T9749 T9719 punct .,performed
R2844 T9751 T9752 nsubj This,resulted
R2845 T9753 T9752 prep in,resulted
R2846 T9754 T9755 nummod two,litters
R2847 T9755 T9753 pobj litters,in
R2848 T9756 T9755 prep of,litters
R2849 T9757 T9756 pobj mice,of
R2850 T9758 T9755 prep with,litters
R2851 T9759 T9760 det a,total
R2852 T9760 T9758 pobj total,with
R2853 T9761 T9760 prep of,total
R2854 T9762 T9763 nummod four,pups
R2855 T9763 T9761 pobj pups,of
R2856 T9764 T9763 amod affected,pups
R2857 T9765 T9766 compound Itpr1opt,Δ18
R2858 T9766 T9763 compound Δ18,pups
R2859 T9767 T9766 punct /,Δ18
R2860 T9768 T9769 punct (,eight
R2861 T9769 T9763 parataxis eight,pups
R2862 T9770 T9769 prep from,eight
R2863 T9771 T9772 det a,total
R2864 T9772 T9770 pobj total,from
R2865 T9773 T9772 prep of,total
R2866 T9774 T9773 pobj 15,of
R2867 T9775 T9769 punct ;,eight
R2868 T9776 T9777 quantmod two,seven
R2869 T9777 T9769 dep seven,eight
R2870 T9778 T9777 quantmod of,seven
R2871 T9779 T9777 prep from,seven
R2872 T9780 T9781 amod first,mating
R2873 T9781 T9779 pobj mating,from
R2874 T9782 T9769 punct ;,eight
R2875 T9783 T9769 quantmod two,eight
R2876 T9784 T9769 quantmod of,eight
R2877 T9785 T9769 prep from,eight
R2878 T9786 T9787 det the,mating
R2879 T9787 T9785 pobj mating,from
R2880 T9788 T9787 amod second,mating
R2881 T9789 T9769 punct ),eight
R2882 T9790 T9763 prep with,pups
R2883 T9791 T9792 det a,phenotype
R2884 T9792 T9790 pobj phenotype,with
R2885 T9793 T9792 amod indistinguishable,phenotype
R2886 T9794 T9793 prep from,indistinguishable
R2887 T9795 T9794 pobj that,from
R2888 T9796 T9795 prep of,that
R2889 T9797 T9798 det the,mice
R2890 T9798 T9796 pobj mice,of
R2891 T9799 T9800 nmod Itpr1Δ18,Δ18
R2892 T9800 T9798 nmod Δ18,mice
R2893 T9801 T9800 punct /,Δ18
R2894 T9802 T9800 cc and,Δ18
R2895 T9803 T9804 compound Itpr1opt,opt
R2896 T9804 T9800 conj opt,Δ18
R2897 T9805 T9804 punct /,opt
R2898 T9806 T9752 punct .,resulted
R2899 T10117 T10116 prep of,Analysis
R2900 T10118 T10119 compound Itpr1,protein
R2901 T10119 T10117 pobj protein,of
R2902 T10120 T10119 prep in,protein
R2903 T10121 T10120 pobj mice,in
R2904 T10122 T10116 punct .,Analysis
R2905 T10124 T10125 nsubj We,performed
R2906 T10126 T10127 compound Western,blot
R2907 T10127 T10128 compound blot,analyses
R2908 T10128 T10125 dobj analyses,performed
R2909 T10129 T10125 advcl using,performed
R2910 T10130 T10131 amod standard,techniques
R2911 T10131 T10129 dobj techniques,using
R2912 T10132 T10125 prep with,performed
R2913 T10133 T10134 compound ECL,kits
R2914 T10134 T10132 pobj kits,with
R2915 T10135 T10134 compound detection,kits
R2916 T10136 T10137 punct (,Amersham
R2917 T10137 T10134 parataxis Amersham,kits
R2918 T10138 T10137 punct ", ",Amersham
R2919 T10139 T10137 npadvmod http://www.amersham.com,Amersham
R2920 T10140 T10137 punct ),Amersham
R2921 T10141 T10125 punct .,performed
R2922 T10143 T10144 advmod Briefly,homogenized
R2923 T10145 T10144 punct ", ",homogenized
R2924 T10146 T10147 amod dissected,brains
R2925 T10147 T10144 nsubjpass brains,homogenized
R2926 T10148 T10147 amod whole,brains
R2927 T10149 T10147 prep from,brains
R2928 T10150 T10151 amod postnatal,day
R2929 T10151 T10152 nmod day,littermates
R2930 T10152 T10149 pobj littermates,from
R2931 T10153 T10151 nummod 21,day
R2932 T10154 T10144 auxpass were,homogenized
R2933 T10155 T10144 prep in,homogenized
R2934 T10156 T10157 det a,buffer
R2935 T10157 T10155 pobj buffer,in
R2936 T10158 T10157 acl containing,buffer
R2937 T10159 T10160 nummod 50,mM
R2938 T10160 T10161 compound mM,HCl
R2939 T10161 T10158 dobj HCl,containing
R2940 T10162 T10161 compound Tris,HCl
R2941 T10163 T10161 punct -,HCl
R2942 T10164 T10161 punct ", ",HCl
R2943 T10165 T10166 nummod 150,mM
R2944 T10166 T10167 compound mM,NaCl
R2945 T10167 T10161 conj NaCl,HCl
R2946 T10168 T10167 punct ", ",NaCl
R2947 T10169 T10170 nummod 1,mM
R2948 T10170 T10171 compound mM,EDTA
R2949 T10171 T10167 conj EDTA,NaCl
R2950 T10172 T10171 punct ", ",EDTA
R2951 T10173 T10174 nummod 1,%
R2952 T10174 T10175 compound %,X
R2953 T10175 T10171 conj X,EDTA
R2954 T10176 T10175 compound Triton,X
R2955 T10177 T10175 punct -,X
R2956 T10178 T10175 nummod 100,X
R2957 T10179 T10175 punct ", ",X
R2958 T10180 T10181 nummod 1,%
R2959 T10181 T10182 compound %,deoxycholate
R2960 T10182 T10175 conj deoxycholate,X
R2961 T10183 T10182 compound sodium,deoxycholate
R2962 T10184 T10182 punct ", ",deoxycholate
R2963 T10185 T10186 nummod 0.1,%
R2964 T10186 T10187 compound %,SDS
R2965 T10187 T10182 conj SDS,deoxycholate
R2966 T10188 T10187 punct ", ",SDS
R2967 T10189 T10187 cc and,SDS
R2968 T10190 T10191 det a,cocktail
R2969 T10191 T10187 conj cocktail,SDS
R2970 T10192 T10191 prep of,cocktail
R2971 T10193 T10194 compound protease,inhibitors
R2972 T10194 T10192 pobj inhibitors,of
R2973 T10195 T10196 punct (,Roche
R2974 T10196 T10191 parataxis Roche,cocktail
R2975 T10197 T10196 punct ", ",Roche
R2976 T10198 T10196 npadvmod http://www.roche.com,Roche
R2977 T10199 T10196 punct ),Roche
R2978 T10200 T10144 punct .,homogenized
R2979 T10202 T10203 nsubjpass Homogenates,diluted
R2980 T10204 T10203 auxpass were,diluted
R2981 T10205 T10203 advmod appropriately,diluted
R2982 T10206 T10203 punct ", ",diluted
R2983 T10207 T10203 conj mixed,diluted
R2984 T10208 T10207 prep with,mixed
R2985 T10209 T10210 nummod 4,buffer
R2986 T10210 T10208 pobj buffer,with
R2987 T10211 T10209 punct ×,4
R2988 T10212 T10210 amod reducing,buffer
R2989 T10213 T10210 compound sample,buffer
R2990 T10214 T10207 punct ", ",mixed
R2991 T10215 T10207 cc and,mixed
R2992 T10216 T10207 conj loaded,mixed
R2993 T10217 T10216 prep onto,loaded
R2994 T10218 T10219 nummod 4,%
R2995 T10219 T10220 nmod %,gels
R2996 T10220 T10217 pobj gels,onto
R2997 T10221 T10222 punct –,%
R2998 T10222 T10219 prep %,%
R2999 T10223 T10222 nummod 12,%
R3000 T10224 T10220 compound precast,gels
R3001 T10225 T10220 compound gradient,gels
R3002 T10226 T10227 punct (,Novex
R3003 T10227 T10220 parataxis Novex,gels
R3004 T10228 T10227 punct ", ",Novex
R3005 T10229 T10227 npadvmod http://www.invitrogen.com,Novex
R3006 T10230 T10227 punct ),Novex
R3007 T10231 T10216 prep for,loaded
R3008 T10232 T10233 compound SDS,PAGE
R3009 T10233 T10231 pobj PAGE,for
R3010 T10234 T10233 punct -,PAGE
R3011 T10235 T10233 cc and,PAGE
R3012 T10236 T10233 conj immunoblotting,PAGE
R3013 T10237 T10203 punct .,diluted
R3014 T10239 T10240 det The,antibodies
R3015 T10240 T10241 nsubjpass antibodies,used
R3016 T10242 T10240 prep to,antibodies
R3017 T10243 T10242 pobj Itpr1,to
R3018 T10244 T10245 punct (,1
R3019 T10245 T10243 parataxis 1,Itpr1
R3020 T10246 T10247 punct :,"2,000"
R3021 T10247 T10245 prep "2,000",1
R3022 T10248 T10245 punct ),1
R3023 T10249 T10243 cc and,Itpr1
R3024 T10250 T10243 conj Actb,Itpr1
R3025 T10251 T10252 punct (,1
R3026 T10252 T10250 parataxis 1,Actb
R3027 T10253 T10254 punct :,"5,000"
R3028 T10254 T10252 prep "5,000",1
R3029 T10255 T10252 punct ),1
R3030 T10256 T10241 auxpass were,used
R3031 T10257 T10258 mark as,recommended
R3032 T10258 T10241 advcl recommended,used
R3033 T10259 T10258 agent by,recommended
R3034 T10260 T10259 pobj manufacturers,by
R3035 T10261 T10241 punct .,used
R3036 T10936 T10935 punct .,Immunohistochemistry
R3037 T10938 T10939 nsubjpass Brains,isolated
R3038 T10940 T10939 auxpass were,isolated
R3039 T10941 T10939 prep from,isolated
R3040 T10942 T10943 nummod 21,d
R3041 T10943 T10945 npadvmod d,old
R3042 T10944 T10943 punct -,d
R3043 T10945 T10947 amod old,mice
R3044 T10946 T10945 punct -,old
R3045 T10947 T10941 pobj mice,from
R3046 T10948 T10939 punct ", ",isolated
R3047 T10949 T10939 conj perfused,isolated
R3048 T10950 T10949 prep with,perfused
R3049 T10951 T10952 nummod 4,%
R3050 T10952 T10953 compound %,paraformaldehyde
R3051 T10953 T10950 pobj paraformaldehyde,with
R3052 T10954 T10953 prep in,paraformaldehyde
R3053 T10955 T10954 pobj PBS,in
R3054 T10956 T10949 punct ", ",perfused
R3055 T10957 T10949 cc and,perfused
R3056 T10958 T10949 conj post-fixed,perfused
R3057 T10959 T10958 advmod overnight,post-fixed
R3058 T10960 T10958 prep in,post-fixed
R3059 T10961 T10962 det the,fixative
R3060 T10962 T10960 pobj fixative,in
R3061 T10963 T10962 amod same,fixative
R3062 T10964 T10939 punct .,isolated
R3063 T10966 T10967 nsubjpass Brains,embedded
R3064 T10968 T10967 auxpass were,embedded
R3065 T10969 T10967 prep in,embedded
R3066 T10970 T10969 pobj gelatin,in
R3067 T10971 T10967 punct ", ",embedded
R3068 T10972 T10967 cc and,embedded
R3069 T10973 T10974 nummod 35,μm
R3070 T10974 T10976 nmod μm,sections
R3071 T10975 T10974 punct -,μm
R3072 T10976 T10978 nsubjpass sections,cut
R3073 T10977 T10976 amod sagittal,sections
R3074 T10978 T10967 conj cut,embedded
R3075 T10979 T10978 auxpass were,cut
R3076 T10980 T10978 advcl using,cut
R3077 T10981 T10982 det a,microtome
R3078 T10982 T10980 dobj microtome,using
R3079 T10983 T10982 amod sliding,microtome
R3080 T10984 T10985 punct (,Associates
R3081 T10985 T10982 parataxis Associates,microtome
R3082 T10986 T10985 compound NeuroScience,Associates
R3083 T10987 T10985 punct ", ",Associates
R3084 T10988 T10985 npadvmod http://www.neuroscienceassociates.com,Associates
R3085 T10989 T10985 punct ),Associates
R3086 T10990 T10978 punct .,cut
R3087 T10992 T10993 nsubjpass Sections,placed
R3088 T10994 T10992 prep from,Sections
R3089 T10995 T10996 amod wild,type
R3090 T10996 T10998 nmod type,brains
R3091 T10997 T10996 punct -,type
R3092 T10998 T10994 pobj brains,from
R3093 T10999 T10996 punct ", ",type
R3094 T11000 T10996 conj heterozygote,type
R3095 T11001 T11000 punct ", ",heterozygote
R3096 T11002 T11000 cc and,heterozygote
R3097 T11003 T11000 conj homozygous,heterozygote
R3098 T11004 T10993 auxpass were,placed
R3099 T11005 T10993 prep in,placed
R3100 T11006 T11007 det the,template
R3101 T11007 T11005 pobj template,in
R3102 T11008 T11007 compound MultiBrain,template
R3103 T11009 T10993 punct .,placed
R3104 T11011 T11012 nsubjpass Sections,washed
R3105 T11013 T11012 auxpass were,washed
R3106 T11014 T11012 prep in,washed
R3107 T11015 T11016 nummod 1,PBS
R3108 T11016 T11014 pobj PBS,in
R3109 T11017 T11015 punct ×,1
R3110 T11018 T11019 advmod prior,to
R3111 T11019 T11012 prep to,washed
R3112 T11020 T11021 nummod 1,h
R3113 T11021 T11019 pobj h,to
R3114 T11022 T11021 prep of,h
R3115 T11023 T11022 pobj incubation,of
R3116 T11024 T11023 prep in,incubation
R3117 T11025 T11026 compound block,solution
R3118 T11026 T11024 pobj solution,in
R3119 T11027 T11026 acl containing,solution
R3120 T11028 T11029 nummod 1,PBS
R3121 T11029 T11027 dobj PBS,containing
R3122 T11030 T11028 punct ×,1
R3123 T11031 T11029 prep with,PBS
R3124 T11032 T11033 nummod 20,%
R3125 T11033 T11034 nmod %,serum
R3126 T11034 T11031 pobj serum,with
R3127 T11035 T11034 amod normal,serum
R3128 T11036 T11034 compound goat,serum
R3129 T11037 T11034 cc and,serum
R3130 T11038 T11039 nummod 0.3,%
R3131 T11039 T11040 compound %,X
R3132 T11040 T11034 conj X,serum
R3133 T11041 T11040 compound Triton,X
R3134 T11042 T11040 punct -,X
R3135 T11043 T11040 nummod 100,X
R3136 T11044 T11045 punct (,pH
R3137 T11045 T11040 parataxis pH,X
R3138 T11046 T11045 nummod 7.4,pH
R3139 T11047 T11045 punct ),pH
R3140 T11048 T11012 punct .,washed
R3141 T11050 T11051 nsubjpass Sections,incubated
R3142 T11052 T11051 auxpass were,incubated
R3143 T11053 T11051 advmod overnight,incubated
R3144 T11054 T11051 prep at,incubated
R3145 T11055 T11056 nummod 4,°C
R3146 T11056 T11054 pobj °C,at
R3147 T11057 T11051 prep in,incubated
R3148 T11058 T11059 amod primary,antibodies
R3149 T11059 T11057 pobj antibodies,in
R3150 T11060 T11059 punct : ,antibodies
R3151 T11061 T11062 nmod affinity,antibody
R3152 T11062 T11059 appos antibody,antibodies
R3153 T11063 T11062 amod purified,antibody
R3154 T11064 T11062 amod polyclonal,antibody
R3155 T11065 T11062 compound Itpr1,antibody
R3156 T11066 T11067 punct (,International
R3157 T11067 T11062 parataxis International,antibody
R3158 T11068 T11067 dep 1,International
R3159 T11069 T11070 punct :,"2,000"
R3160 T11070 T11068 prep "2,000",1
R3161 T11071 T11067 punct ", ",International
R3162 T11072 T11067 compound Chemicon,International
R3163 T11073 T11067 punct ", ",International
R3164 T11074 T11067 npadvmod http://www.chemicon.com,International
R3165 T11075 T11067 punct ),International
R3166 T11076 T11062 cc and,antibody
R3167 T11077 T11078 amod monoclonal,antibody
R3168 T11078 T11062 conj antibody,antibody
R3169 T11079 T11078 amod anti-Calb1,antibody
R3170 T11080 T11081 punct (,Aldrich
R3171 T11081 T11078 parataxis Aldrich,antibody
R3172 T11082 T11081 dep 1,Aldrich
R3173 T11083 T11084 punct :,"6,000"
R3174 T11084 T11082 prep "6,000",1
R3175 T11085 T11081 punct ", ",Aldrich
R3176 T11086 T11081 compound Sigma,Aldrich
R3177 T11087 T11081 punct -,Aldrich
R3178 T11088 T11081 punct ", ",Aldrich
R3179 T11089 T11081 npadvmod http://www.sigmaaldrich.com,Aldrich
R3180 T11090 T11081 punct ),Aldrich
R3181 T11091 T11062 acl diluted,antibody
R3182 T11092 T11091 prep in,diluted
R3183 T11093 T11094 compound carrier,solution
R3184 T11094 T11092 pobj solution,in
R3185 T11095 T11051 punct .,incubated
R3186 T11097 T11098 prep Following,incubated
R3187 T11099 T11100 amod extensive,washes
R3188 T11100 T11097 pobj washes,Following
R3189 T11101 T11100 punct (,washes
R3190 T11102 T11100 prep in,washes
R3191 T11103 T11104 nummod 6.0,ml
R3192 T11104 T11102 pobj ml,in
R3193 T11105 T11104 prep of,ml
R3194 T11106 T11105 pobj PBS,of
R3195 T11107 T11108 punct ", ",times
R3196 T11108 T11100 parataxis times,washes
R3197 T11109 T11108 nummod three,times
R3198 T11110 T11108 punct ),times
R3199 T11111 T11098 punct ", ",incubated
R3200 T11112 T11098 nsubjpass sections,incubated
R3201 T11113 T11098 auxpass were,incubated
R3202 T11114 T11098 prep with,incubated
R3203 T11115 T11116 amod appropriate,antibodies
R3204 T11116 T11114 pobj antibodies,with
R3205 T11117 T11116 amod secondary,antibodies
R3206 T11118 T11119 punct (,IgG
R3207 T11119 T11116 parataxis IgG,antibodies
R3208 T11120 T11121 nmod Alexa,Fluor
R3209 T11121 T11119 nmod Fluor,IgG
R3210 T11122 T11121 nummod 555,Fluor
R3211 T11123 T11119 nmod goat,IgG
R3212 T11124 T11119 amod anti-rabbit,IgG
R3213 T11125 T11119 cc and,IgG
R3214 T11126 T11127 nmod Alexa,Fluor
R3215 T11127 T11128 nmod Fluor,IgG
R3216 T11128 T11119 conj IgG,IgG
R3217 T11129 T11127 nummod 488,Fluor
R3218 T11130 T11128 nmod goat,IgG
R3219 T11131 T11128 amod anti-mouse,IgG
R3220 T11132 T11133 punct [,Invitrogen
R3221 T11133 T11128 parataxis Invitrogen,IgG
R3222 T11134 T11133 punct ", ",Invitrogen
R3223 T11135 T11133 npadvmod http://www.invitrogen.com,Invitrogen
R3224 T11136 T11133 punct ],Invitrogen
R3225 T11137 T11119 punct ),IgG
R3226 T11138 T11098 prep for,incubated
R3227 T11139 T11140 nummod 1,h
R3228 T11140 T11138 pobj h,for
R3229 T11141 T11098 prep at,incubated
R3230 T11142 T11143 compound room,temperature
R3231 T11143 T11141 pobj temperature,at
R3232 T11144 T11098 punct .,incubated
R3233 T11146 T11147 nsubjpass Sections,washed
R3234 T11148 T11147 auxpass were,washed
R3235 T11149 T11147 cc and,washed
R3236 T11150 T11147 conj mounted,washed
R3237 T11151 T11150 prep on,mounted
R3238 T11152 T11153 compound glass,slides
R3239 T11153 T11151 pobj slides,on
R3240 T11154 T11150 prep in,mounted
R3241 T11155 T11156 det a,medium
R3242 T11156 T11154 pobj medium,in
R3243 T11157 T11156 amod buffered,medium
R3244 T11158 T11156 acl containing,medium
R3245 T11159 T11158 dobj Mowiol,containing
R3246 T11160 T11161 punct (,Calbiochem
R3247 T11161 T11159 parataxis Calbiochem,Mowiol
R3248 T11162 T11161 punct ", ",Calbiochem
R3249 T11163 T11161 npadvmod http://www.emdbiosciences.com,Calbiochem
R3250 T11164 T11161 punct ),Calbiochem
R3251 T11165 T11166 mark as,described
R3252 T11166 T11150 advcl described,mounted
R3253 T11167 T11166 advmod earlier,described
R3254 T11168 T11169 punct [,14
R3255 T11169 T11150 parataxis 14,mounted
R3256 T11170 T11169 punct ],14
R3257 T11171 T11147 punct .,washed
R3258 T11173 T11174 nsubjpass Sections,imaged
R3259 T11175 T11174 auxpass were,imaged
R3260 T11176 T11174 advcl using,imaged
R3261 T11177 T11178 det a,microscope
R3262 T11178 T11176 dobj microscope,using
R3263 T11179 T11178 nmod laser,microscope
R3264 T11180 T11178 amod scanning,microscope
R3265 T11181 T11178 amod confocal,microscope
R3266 T11182 T11183 punct (,Zeiss
R3267 T11183 T11178 parataxis Zeiss,microscope
R3268 T11184 T11183 dep LSM,Zeiss
R3269 T11185 T11184 nummod 510,LSM
R3270 T11186 T11183 punct ;,Zeiss
R3271 T11187 T11183 punct ", ",Zeiss
R3272 T11188 T11183 npadvmod http://www.zeiss.com,Zeiss
R3273 T11189 T11183 punct ),Zeiss
R3274 T11190 T11174 punct .,imaged
R3275 T11192 T11193 compound Imaging,parameters
R3276 T11193 T11194 nsubjpass parameters,optimized
R3277 T11195 T11196 punct (,pinhole
R3278 T11196 T11193 parataxis pinhole,parameters
R3279 T11197 T11196 punct ", ",pinhole
R3280 T11198 T11199 compound detector,gain
R3281 T11199 T11196 appos gain,pinhole
R3282 T11200 T11196 punct ", ",pinhole
R3283 T11201 T11202 compound laser,power
R3284 T11202 T11196 appos power,pinhole
R3285 T11203 T11196 punct ),pinhole
R3286 T11204 T11194 auxpass were,optimized
R3287 T11205 T11194 punct ", ",optimized
R3288 T11206 T11194 cc and,optimized
R3289 T11207 T11208 auxpass were,kept
R3290 T11208 T11194 conj kept,optimized
R3291 T11209 T11208 oprd constant,kept
R3292 T11210 T11208 prep for,kept
R3293 T11211 T11212 det the,brains
R3294 T11212 T11210 pobj brains,for
R3295 T11213 T11214 amod wild,type
R3296 T11214 T11212 nmod type,brains
R3297 T11215 T11214 punct -,type
R3298 T11216 T11214 punct ", ",type
R3299 T11217 T11214 conj heterozygous,type
R3300 T11218 T11217 punct ", ",heterozygous
R3301 T11219 T11217 cc and,heterozygous
R3302 T11220 T11217 conj homozygous,heterozygous
R3303 T11221 T11212 compound mutant,brains
R3304 T11222 T11194 punct .,optimized
R3305 T11224 T11225 nsubjpass Specificity,verified
R3306 T11226 T11224 prep of,Specificity
R3307 T11227 T11228 det the,antibodies
R3308 T11228 T11226 pobj antibodies,of
R3309 T11229 T11228 compound Itpr1,antibodies
R3310 T11230 T11225 auxpass was,verified
R3311 T11231 T11225 prep by,verified
R3312 T11232 T11233 compound preabsorption,experiments
R3313 T11233 T11231 pobj experiments,by
R3314 T11234 T11233 compound control,experiments
R3315 T11235 T11225 punct .,verified
R3316 T11237 T11238 compound Antibody,dilutions
R3317 T11238 T11239 nsubjpass dilutions,incubated
R3318 T11240 T11239 auxpass were,incubated
R3319 T11241 T11239 prep for,incubated
R3320 T11242 T11243 nummod 24,h
R3321 T11243 T11241 pobj h,for
R3322 T11244 T11239 prep at,incubated
R3323 T11245 T11246 nummod 4,°C
R3324 T11246 T11244 pobj °C,at
R3325 T11247 T11239 prep with,incubated
R3326 T11248 T11249 det the,peptide
R3327 T11249 T11247 pobj peptide,with
R3328 T11250 T11249 amod immunizing,peptide
R3329 T11251 T11239 punct .,incubated
R3330 T11253 T11254 compound Tissue,sections
R3331 T11254 T11255 nsubjpass sections,incubated
R3332 T11256 T11255 auxpass were,incubated
R3333 T11257 T11255 prep with,incubated
R3334 T11258 T11259 det the,antibodies
R3335 T11259 T11257 pobj antibodies,with
R3336 T11260 T11259 amod preabsorbed,antibodies
R3337 T11261 T11255 cc and,incubated
R3338 T11262 T11255 conj processed,incubated
R3339 T11263 T11264 mark as,described
R3340 T11264 T11262 advcl described,processed
R3341 T11265 T11264 advmod above,described
R3342 T11266 T11255 punct .,incubated
R3343 T11268 T11269 prep Under,detected
R3344 T11270 T11271 det these,conditions
R3345 T11271 T11268 pobj conditions,Under
R3346 T11272 T11269 punct ", ",detected
R3347 T11273 T11274 det no,staining
R3348 T11274 T11269 nsubjpass staining,detected
R3349 T11275 T11274 prep above,staining
R3350 T11276 T11275 pobj autofluorescence,above
R3351 T11277 T11269 auxpass was,detected
R3352 T11278 T11269 punct .,detected
R3355 T12756 T12755 prep of,Analysis
R3356 T12757 T12756 pobj ITPR1,of
R3357 T12758 T12757 prep in,ITPR1
R3358 T12759 T12760 compound SCA15,patients
R3359 T12760 T12758 pobj patients,in
R3360 T12761 T12755 punct .,Analysis
R3361 T12763 T12764 nsubjpass DNA,extracted
R3362 T12765 T12764 auxpass was,extracted
R3363 T12766 T12764 prep from,extracted
R3364 T12767 T12768 nmod EBV,lymphocytes
R3365 T12768 T12766 pobj lymphocytes,from
R3366 T12769 T12768 amod immortalized,lymphocytes
R3367 T12770 T12768 punct ", ",lymphocytes
R3368 T12771 T12768 acl derived,lymphocytes
R3369 T12772 T12771 prep from,derived
R3370 T12773 T12774 compound family,members
R3371 T12774 T12772 pobj members,from
R3372 T12775 T12764 punct .,extracted
R3373 T12777 T12778 det The,exons
R3374 T12778 T12780 nsubjpass exons,amplified
R3375 T12779 T12778 amod coding,exons
R3376 T12781 T12778 cc and,exons
R3377 T12782 T12783 advmod at,50
R3378 T12783 T12785 nummod 50,bp
R3379 T12784 T12783 advmod least,50
R3380 T12785 T12778 conj bp,exons
R3381 T12786 T12785 prep of,bp
R3382 T12787 T12788 amod flanking,introns
R3383 T12788 T12786 pobj introns,of
R3384 T12789 T12788 prep of,introns
R3385 T12790 T12789 pobj ITPR1,of
R3386 T12791 T12780 auxpass were,amplified
R3387 T12792 T12780 dep PCR,amplified
R3388 T12793 T12780 cc and,amplified
R3389 T12794 T12780 conj sequenced,amplified
R3390 T12795 T12794 advcl using,sequenced
R3391 T12796 T12797 compound dye,terminator
R3392 T12797 T12799 compound terminator,sequencing
R3393 T12798 T12797 punct -,terminator
R3394 T12799 T12795 dobj sequencing,using
R3395 T12800 T12801 punct (,Biosystems
R3396 T12801 T12799 parataxis Biosystems,sequencing
R3397 T12802 T12803 compound BigDye,version
R3398 T12803 T12801 dep version,Biosystems
R3399 T12804 T12803 nummod 3.1,version
R3400 T12805 T12801 punct ;,Biosystems
R3401 T12806 T12801 compound Applied,Biosystems
R3402 T12807 T12801 punct ", ",Biosystems
R3403 T12808 T12801 npadvmod http://www.appliedbiosystems.com,Biosystems
R3404 T12809 T12801 punct ),Biosystems
R3405 T12810 T12780 punct .,amplified
R3406 T12812 T12813 compound Sequence,reactions
R3407 T12813 T12814 nsubjpass reactions,run
R3408 T12815 T12814 auxpass were,run
R3409 T12816 T12814 prep on,run
R3410 T12817 T12818 det an,sequencer
R3411 T12818 T12816 pobj sequencer,on
R3412 T12819 T12818 nmod ABI3730XP,sequencer
R3413 T12820 T12818 amod automated,sequencer
R3414 T12821 T12814 prep as,run
R3415 T12822 T12821 prep per,as
R3416 T12823 T12824 det the,manufacturer
R3417 T12824 T12825 poss manufacturer,instructions
R3418 T12825 T12822 pobj instructions,per
R3419 T12826 T12824 case 's,manufacturer
R3420 T12827 T12828 punct (,Biosystems
R3421 T12828 T12825 parataxis Biosystems,instructions
R3422 T12829 T12828 compound Applied,Biosystems
R3423 T12830 T12828 punct ),Biosystems
R3424 T12831 T12814 punct .,run
R3425 T12833 T12834 det This,analysis
R3426 T12834 T12835 nsubjpass analysis,performed
R3427 T12836 T12835 auxpass was,performed
R3428 T12837 T12835 prep in,performed
R3429 T12838 T12839 det all,members
R3430 T12839 T12837 pobj members,in
R3431 T12840 T12839 nummod three,members
R3432 T12841 T12839 amod affected,members
R3433 T12842 T12839 compound family,members
R3434 T12843 T12844 prep for,was
R3435 T12844 T12839 relcl was,members
R3436 T12845 T12843 pobj whom,for
R3437 T12846 T12847 amod genomic,DNA
R3438 T12847 T12844 nsubj DNA,was
R3439 T12848 T12844 acomp available,was
R3440 T12849 T12850 punct (,6
R3441 T12850 T12839 parataxis 6,members
R3442 T12851 T12850 nmod members,6
R3443 T12852 T12850 punct ", ",6
R3444 T12853 T12850 conj 7,6
R3445 T12854 T12853 punct ", ",7
R3446 T12855 T12853 cc and,7
R3447 T12856 T12853 conj 19,7
R3448 T12857 T12850 punct ),6
R3449 T12858 T12835 punct .,performed
R3450 T12860 T12861 compound Primer,sequences
R3451 T12861 T12862 nsubj sequences,are
R3452 T12863 T12861 cc and,sequences
R3453 T12864 T12861 conj conditions,sequences
R3454 T12865 T12862 acomp available,are
R3455 T12866 T12862 prep upon,are
R3456 T12867 T12866 pobj request,upon
R3457 T12868 T12862 punct .,are
R3458 T12870 T12871 compound Sequence,data
R3459 T12871 T12872 nsubjpass data,analyzed
R3460 T12873 T12872 auxpass were,analyzed
R3461 T12874 T12872 advcl using,analyzed
R3462 T12875 T12874 dobj Sequencher,using
R3463 T12876 T12877 punct (,Corporation
R3464 T12877 T12875 parataxis Corporation,Sequencher
R3465 T12878 T12879 compound Gene,Codes
R3466 T12879 T12877 compound Codes,Corporation
R3467 T12880 T12877 punct ", ",Corporation
R3468 T12881 T12877 npadvmod http://www.genecodes.com,Corporation
R3469 T12882 T12877 punct ),Corporation
R3470 T12883 T12872 punct .,analyzed
R3471 T12885 T12886 npadvmod Genome,wide
R3472 T12886 T12888 amod wide,genotyping
R3473 T12887 T12886 punct -,wide
R3474 T12888 T12890 nsubjpass genotyping,performed
R3475 T12889 T12888 compound SNP,genotyping
R3476 T12891 T12890 auxpass was,performed
R3477 T12892 T12890 advcl using,performed
R3478 T12893 T12894 compound Infinium,SNP
R3479 T12894 T12896 compound SNP,chips
R3480 T12895 T12894 compound HumanHap550,SNP
R3481 T12896 T12892 dobj chips,using
R3482 T12897 T12896 compound genotyping,chips
R3483 T12898 T12892 prep as,using
R3484 T12899 T12898 prep per,as
R3485 T12900 T12901 det the,manufacturer
R3486 T12901 T12902 poss manufacturer,protocol
R3487 T12902 T12899 pobj protocol,per
R3488 T12903 T12901 case 's,manufacturer
R3489 T12904 T12905 punct (,Illumina
R3490 T12905 T12902 parataxis Illumina,protocol
R3491 T12906 T12905 punct ", ",Illumina
R3492 T12907 T12905 npadvmod http://www.illumina.com,Illumina
R3493 T12908 T12905 punct ),Illumina
R3494 T12909 T12890 punct .,performed
R3495 T12911 T12912 det This,product
R3496 T12912 T12913 nsubj product,assays
R3497 T12914 T12915 nummod "555,352",SNPs
R3498 T12915 T12913 dobj SNPs,assays
R3499 T12916 T12915 amod unique,SNPs
R3500 T12917 T12913 punct .,assays
R3501 T12919 T12920 nsubjpass Data,collected
R3502 T12921 T12920 auxpass were,collected
R3503 T12922 T12920 advcl using,collected
R3504 T12923 T12924 det the,scanner
R3505 T12924 T12922 dobj scanner,using
R3506 T12925 T12926 compound Illumina,BeadStation
R3507 T12926 T12924 compound BeadStation,scanner
R3508 T12927 T12924 cc and,scanner
R3509 T12928 T12929 compound data,collection
R3510 T12929 T12930 compound collection,software
R3511 T12930 T12924 conj software,scanner
R3512 T12931 T12920 punct .,collected
R3513 T12933 T12934 nsubjpass Genotypes,produced
R3514 T12935 T12934 auxpass were,produced
R3515 T12936 T12934 advcl using,produced
R3516 T12937 T12938 det the,module
R3517 T12938 T12936 dobj module,using
R3518 T12939 T12938 compound genotyping,module
R3519 T12940 T12938 prep of,module
R3520 T12941 T12940 pobj BeadStudio,of
R3521 T12942 T12943 punct (,version
R3522 T12943 T12941 parataxis version,BeadStudio
R3523 T12944 T12943 nummod 2.3.25,version
R3524 T12945 T12943 punct ;,version
R3525 T12946 T12943 appos Illumina,version
R3526 T12947 T12943 punct ),version
R3527 T12948 T12934 punct ", ",produced
R3528 T12949 T12934 cc and,produced
R3529 T12950 T12951 compound log,R
R3530 T12951 T12952 compound R,ratio
R3531 T12952 T12953 nsubjpass ratio,visualized
R3532 T12953 T12934 conj visualized,produced
R3533 T12954 T12952 cc and,ratio
R3534 T12955 T12956 compound B,allele
R3535 T12956 T12957 compound allele,frequency
R3536 T12957 T12952 conj frequency,ratio
R3537 T12958 T12953 auxpass were,visualized
R3538 T12959 T12953 advcl using,visualized
R3539 T12960 T12961 det the,tool
R3540 T12961 T12959 dobj tool,using
R3541 T12962 T12961 nmod genome,tool
R3542 T12963 T12962 amod viewer,genome
R3543 T12964 T12961 prep within,tool
R3544 T12965 T12966 det this,package
R3545 T12966 T12964 pobj package,within
R3546 T12967 T12953 punct .,visualized
R3547 T12969 T12970 prep In,examined
R3548 T12971 T12969 pobj order,In
R3549 T12972 T12973 aux to,rule
R3550 T12973 T12971 acl rule,order
R3551 T12974 T12973 prt out,rule
R3552 T12975 T12976 det the,possibility
R3553 T12976 T12973 dobj possibility,rule
R3554 T12977 T12978 mark that,was
R3555 T12978 T12976 acl was,possibility
R3556 T12979 T12980 det the,deletion
R3557 T12980 T12978 nsubj deletion,was
R3558 T12981 T12980 amod observed,deletion
R3559 T12982 T12980 prep within,deletion
R3560 T12983 T12982 pobj ITPR1,within
R3561 T12984 T12985 det a,variant
R3562 T12985 T12978 attr variant,was
R3563 T12986 T12985 amod benign,variant
R3564 T12987 T12985 compound copy,variant
R3565 T12988 T12985 compound number,variant
R3566 T12989 T12970 nsubj we,examined
R3567 T12990 T12991 nmod log,R
R3568 T12991 T12992 nmod R,ratio
R3569 T12992 T12993 nmod ratio,metrics
R3570 T12993 T12970 dobj metrics,examined
R3571 T12994 T12992 cc and,ratio
R3572 T12995 T12996 compound B,allele
R3573 T12996 T12997 compound allele,frequency
R3574 T12997 T12992 conj frequency,ratio
R3575 T12998 T12993 prep of,metrics
R3576 T12999 T13000 compound HumanHap550,data
R3577 T13000 T12998 pobj data,of
R3578 T13001 T13000 compound genotyping,data
R3579 T13002 T13000 prep at,data
R3580 T13003 T13004 det this,locus
R3581 T13004 T13002 pobj locus,at
R3582 T13005 T13000 prep from,data
R3583 T13006 T13007 nummod 577,individuals
R3584 T13007 T13005 pobj individuals,from
R3585 T13008 T13007 prep of,individuals
R3586 T13009 T13010 amod Northern,European
R3587 T13010 T13011 amod European,descent
R3588 T13011 T13008 pobj descent,of
R3589 T13012 T13007 prep from,individuals
R3590 T13013 T13014 compound North,America
R3591 T13014 T13012 pobj America,from
R3592 T13015 T13014 cc and,America
R3593 T13016 T13014 conj Europe,America
R3594 T13017 T13000 punct ", ",data
R3595 T13018 T13000 acl produced,data
R3596 T13019 T13018 agent by,produced
R3597 T13020 T13019 pobj us,by
R3598 T13021 T13018 prep as,produced
R3599 T13022 T13023 det a,part
R3600 T13023 T13021 pobj part,as
R3601 T13024 T13023 prep of,part
R3602 T13025 T13026 det an,study
R3603 T13026 T13024 pobj study,of
R3604 T13027 T13026 amod ongoing,study
R3605 T13028 T12970 punct .,examined
R3606 T13030 T13031 prep In,designed
R3607 T13032 T13033 det an,attempt
R3608 T13033 T13030 pobj attempt,In
R3609 T13034 T13035 aux to,narrow
R3610 T13035 T13033 acl narrow,attempt
R3611 T13036 T13037 det the,intervals
R3612 T13037 T13035 dobj intervals,narrow
R3613 T13038 T13037 amod unknown,intervals
R3614 T13039 T13037 acl flanking,intervals
R3615 T13040 T13041 det the,deletion
R3616 T13041 T13039 dobj deletion,flanking
R3617 T13042 T13041 acl observed,deletion
R3618 T13043 T13042 prep in,observed
R3619 T13044 T13045 compound family,AUS1
R3620 T13045 T13043 pobj AUS1,in
R3621 T13046 T13031 punct ", ",designed
R3622 T13047 T13031 nsubj we,designed
R3623 T13048 T13031 dobj primers,designed
R3624 T13049 T13048 prep for,primers
R3625 T13050 T13051 nummod 30,amplifications
R3626 T13051 T13049 pobj amplifications,for
R3627 T13052 T13051 compound PCR,amplifications
R3628 T13053 T13054 dep that,generate
R3629 T13054 T13051 relcl generate,amplifications
R3630 T13055 T13054 aux would,generate
R3631 T13056 T13057 amod overlapping,fragments
R3632 T13057 T13054 dobj fragments,generate
R3633 T13058 T13054 prep across,generate
R3634 T13059 T13060 det the,regions
R3635 T13060 T13058 pobj regions,across
R3636 T13061 T13060 nummod two,regions
R3637 T13062 T13060 compound bordering,regions
R3638 T13063 T13064 punct (,sequence
R3639 T13064 T13060 parataxis sequence,regions
R3640 T13065 T13064 compound primer,sequence
R3641 T13066 T13064 cc and,sequence
R3642 T13067 T13064 conj conditions,sequence
R3643 T13068 T13064 amod available,sequence
R3644 T13069 T13068 prep upon,available
R3645 T13070 T13069 pobj request,upon
R3646 T13071 T13064 punct ),sequence
R3647 T13072 T13031 punct .,designed
R3648 T13074 T13075 expl There,were
R3649 T13076 T13077 nummod ten,pairs
R3650 T13077 T13075 attr pairs,were
R3651 T13078 T13077 compound primer,pairs
R3652 T13079 T13077 prep in,pairs
R3653 T13080 T13081 det the,region
R3654 T13081 T13079 pobj region,in
R3655 T13082 T13081 amod telomeric,region
R3656 T13083 T13081 compound flanking,region
R3657 T13084 T13077 cc and,pairs
R3658 T13085 T13086 nummod 20,pairs
R3659 T13086 T13077 conj pairs,pairs
R3660 T13087 T13086 prep in,pairs
R3661 T13088 T13089 det the,region
R3662 T13089 T13087 pobj region,in
R3663 T13090 T13089 amod centromeric,region
R3664 T13091 T13089 compound flanking,region
R3665 T13092 T13093 punct (,S3
R3666 T13093 T13075 parataxis S3,were
R3667 T13094 T13093 compound Figure,S3
R3668 T13095 T13093 punct ),S3
R3669 T13096 T13075 punct .,were
R3670 T13098 T13099 prep On,was
R3671 T13100 T13098 amod average,On
R3672 T13101 T13102 det each,product
R3673 T13102 T13099 nsubj product,was
R3674 T13103 T13104 punct ~,750
R3675 T13104 T13105 nummod 750,bp
R3676 T13105 T13099 attr bp,was
R3677 T13106 T13105 prep in,bp
R3678 T13107 T13106 pobj size,in
R3679 T13108 T13099 punct ", ",was
R3680 T13109 T13099 cc and,was
R3681 T13110 T13111 nsubjpass amplifications,performed
R3682 T13111 T13099 conj performed,was
R3683 T13112 T13111 auxpass were,performed
R3684 T13113 T13111 advcl using,performed
R3685 T13114 T13115 amod genomic,DNA
R3686 T13115 T13113 dobj DNA,using
R3687 T13116 T13113 prep from,using
R3688 T13117 T13116 pobj each,from
R3689 T13118 T13117 prep of,each
R3690 T13119 T13120 det the,individuals
R3691 T13120 T13118 pobj individuals,of
R3692 T13121 T13120 nummod three,individuals
R3693 T13122 T13120 amod affected,individuals
R3694 T13123 T13124 punct (,6
R3695 T13124 T13120 parataxis 6,individuals
R3696 T13125 T13124 nmod family,6
R3697 T13126 T13124 nmod members,6
R3698 T13127 T13124 punct ", ",6
R3699 T13128 T13124 conj 7,6
R3700 T13129 T13128 punct ", ",7
R3701 T13130 T13128 cc and,7
R3702 T13131 T13128 conj 19,7
R3703 T13132 T13124 punct ),6
R3704 T13133 T13111 punct .,performed
R3705 T13135 T13136 compound Dye,terminator
R3706 T13136 T13138 compound terminator,sequencing
R3707 T13137 T13136 punct -,terminator
R3708 T13138 T13139 nsubjpass sequencing,performed
R3709 T13139 T13144 ccomp performed,performed
R3710 T13140 T13138 prep of,sequencing
R3711 T13141 T13142 det each,product
R3712 T13142 T13140 pobj product,of
R3713 T13143 T13139 auxpass was,performed
R3714 T13145 T13139 advcl using,performed
R3715 T13146 T13147 det the,primers
R3716 T13147 T13145 dobj primers,using
R3717 T13148 T13147 amod forward,primers
R3718 T13149 T13148 cc and,forward
R3719 T13150 T13148 conj reverse,forward
R3720 T13151 T13147 acl designed,primers
R3721 T13152 T13151 prep for,designed
R3722 T13153 T13152 pobj amplification,for
R3723 T13154 T13144 punct ;,performed
R3724 T13155 T13156 amod running,analysis
R3725 T13156 T13144 nsubjpass analysis,performed
R3726 T13157 T13156 cc and,analysis
R3727 T13158 T13156 prep of,analysis
R3728 T13159 T13160 det each,fragment
R3729 T13160 T13158 pobj fragment,of
R3730 T13161 T13144 auxpass was,performed
R3731 T13162 T13163 mark as,described
R3732 T13163 T13144 advcl described,performed
R3733 T13164 T13163 advmod above,described
R3734 T13165 T13144 punct .,performed
R3735 T13167 T13168 nsubjpass Amplification,denoted
R3736 T13168 T13178 ccomp denoted,inferred
R3737 T13169 T13167 prep of,Amplification
R3738 T13170 T13171 det a,fragment
R3739 T13171 T13169 pobj fragment,of
R3740 T13172 T13171 prep from,fragment
R3741 T13173 T13174 det a,genome
R3742 T13174 T13172 pobj genome,from
R3743 T13175 T13174 amod normal,genome
R3744 T13176 T13174 amod diploid,genome
R3745 T13177 T13168 auxpass was,denoted
R3746 T13179 T13168 agent by,denoted
R3747 T13180 T13181 det the,presence
R3748 T13181 T13179 pobj presence,by
R3749 T13182 T13181 prep of,presence
R3750 T13183 T13184 det a,polymorphism
R3751 T13184 T13182 pobj polymorphism,of
R3752 T13185 T13184 amod heterozygous,polymorphism
R3753 T13186 T13178 punct ;,inferred
R3754 T13187 T13178 nsubjpass amplification,inferred
R3755 T13188 T13187 prep of,amplification
R3756 T13189 T13190 det a,fragment
R3757 T13190 T13188 pobj fragment,of
R3758 T13191 T13190 prep from,fragment
R3759 T13192 T13193 det a,region
R3760 T13193 T13191 pobj region,from
R3761 T13194 T13193 prep of,region
R3762 T13195 T13196 det the,genome
R3763 T13196 T13194 pobj genome,of
R3764 T13197 T13196 acl harboring,genome
R3765 T13198 T13199 det a,deletion
R3766 T13199 T13197 dobj deletion,harboring
R3767 T13200 T13199 amod heterozygous,deletion
R3768 T13201 T13199 amod genomic,deletion
R3769 T13202 T13178 auxpass was,inferred
R3770 T13203 T13204 advmod when,noted
R3771 T13204 T13178 advcl noted,inferred
R3772 T13205 T13204 nsubjpass homozygosity,noted
R3773 T13206 T13205 prep for,homozygosity
R3774 T13207 T13208 det the,allele
R3775 T13208 T13206 pobj allele,for
R3776 T13209 T13208 amod major,allele
R3777 T13210 T13208 cc and,allele
R3778 T13211 T13212 det the,allele
R3779 T13212 T13208 conj allele,allele
R3780 T13213 T13212 amod minor,allele
R3781 T13214 T13204 auxpass were,noted
R3782 T13215 T13204 prep among,noted
R3783 T13216 T13217 det the,members
R3784 T13217 T13215 pobj members,among
R3785 T13218 T13217 nummod three,members
R3786 T13219 T13217 amod affected,members
R3787 T13220 T13217 compound family,members
R3788 T13221 T13222 punct (,is
R3789 T13222 T13204 parataxis is,noted
R3790 T13223 T13222 advmod i.e.,is
R3791 T13224 T13222 punct ", ",is
R3792 T13225 T13222 nsubj this,is
R3793 T13226 T13222 acomp inconsistent,is
R3794 T13227 T13226 prep with,inconsistent
R3795 T13228 T13229 amod Mendelian,inheritance
R3796 T13229 T13227 pobj inheritance,with
R3797 T13230 T13229 prep in,inheritance
R3798 T13231 T13232 amod related,individuals
R3799 T13232 T13230 pobj individuals,in
R3800 T13233 T13232 acl known,individuals
R3801 T13234 T13235 aux to,share
R3802 T13235 T13233 xcomp share,known
R3803 T13236 T13237 det a,haplotype
R3804 T13237 T13235 dobj haplotype,share
R3805 T13238 T13237 amod common,haplotype
R3806 T13239 T13222 punct ),is
R3807 T13240 T13178 punct .,inferred
R3808 T13242 T13243 advcl Using,were
R3809 T13244 T13245 det the,data
R3810 T13245 T13242 dobj data,Using
R3811 T13246 T13242 prep from,Using
R3812 T13247 T13248 det the,experiments
R3813 T13248 T13246 pobj experiments,from
R3814 T13249 T13248 acl described,experiments
R3815 T13250 T13249 advmod above,described
R3816 T13251 T13243 nsubj we,were
R3817 T13252 T13243 acomp able,were
R3818 T13253 T13254 aux to,limit
R3819 T13254 T13252 xcomp limit,able
R3820 T13255 T13256 det the,size
R3821 T13256 T13254 dobj size,limit
R3822 T13257 T13256 prep of,size
R3823 T13258 T13259 amod unknown,regions
R3824 T13259 T13257 pobj regions,of
R3825 T13260 T13259 acl flanking,regions
R3826 T13261 T13262 det the,deletion
R3827 T13262 T13260 dobj deletion,flanking
R3828 T13263 T13254 prep to,limit
R3829 T13264 T13265 punct ~,4
R3830 T13265 T13266 nummod 4,kb
R3831 T13266 T13263 pobj kb,to
R3832 T13267 T13266 prep on,kb
R3833 T13268 T13269 det the,side
R3834 T13269 T13267 pobj side,on
R3835 T13270 T13269 amod telomeric,side
R3836 T13271 T13266 cc and,kb
R3837 T13272 T13273 nummod 7,kb
R3838 T13273 T13266 conj kb,kb
R3839 T13274 T13273 prep on,kb
R3840 T13275 T13276 det the,side
R3841 T13276 T13274 pobj side,on
R3842 T13277 T13276 amod centromeric,side
R3843 T13278 T13243 punct .,were
R3844 T13280 T13281 det All,combinations
R3845 T13281 T13282 nsubjpass combinations,used
R3846 T13283 T13281 prep of,combinations
R3847 T13284 T13285 amod forward,primers
R3848 T13285 T13283 pobj primers,of
R3849 T13286 T13285 prep from,primers
R3850 T13287 T13288 det the,region
R3851 T13288 T13286 pobj region,from
R3852 T13289 T13290 advmod newly,defined
R3853 T13290 T13288 amod defined,region
R3854 T13291 T13281 acl flanking,combinations
R3855 T13292 T13293 det the,deletion
R3856 T13293 T13291 dobj deletion,flanking
R3857 T13294 T13291 prep on,flanking
R3858 T13295 T13296 det the,side
R3859 T13296 T13294 pobj side,on
R3860 T13297 T13296 amod telomeric,side
R3861 T13298 T13291 prep with,flanking
R3862 T13299 T13300 amod reverse,primers
R3863 T13300 T13298 pobj primers,with
R3864 T13301 T13300 prep from,primers
R3865 T13302 T13303 det the,region
R3866 T13303 T13301 pobj region,from
R3867 T13304 T13305 advmod newly,defined
R3868 T13305 T13303 amod defined,region
R3869 T13306 T13300 acl flanking,primers
R3870 T13307 T13308 det the,deletion
R3871 T13308 T13306 dobj deletion,flanking
R3872 T13309 T13306 prep on,flanking
R3873 T13310 T13311 det the,side
R3874 T13311 T13309 pobj side,on
R3875 T13312 T13311 amod centromeric,side
R3876 T13313 T13282 auxpass were,used
R3877 T13314 T13282 prep in,used
R3878 T13315 T13316 compound PCR,amplification
R3879 T13316 T13317 compound amplification,reactions
R3880 T13317 T13314 pobj reactions,in
R3881 T13318 T13317 acl performed,reactions
R3882 T13319 T13318 prep with,performed
R3883 T13320 T13319 pobj DNA,with
R3884 T13321 T13320 prep from,DNA
R3885 T13322 T13323 det the,members
R3886 T13323 T13321 pobj members,from
R3887 T13324 T13323 nummod three,members
R3888 T13325 T13323 amod affected,members
R3889 T13326 T13323 compound family,members
R3890 T13327 T13323 cc and,members
R3891 T13328 T13329 amod single,members
R3892 T13329 T13323 conj members,members
R3893 T13330 T13329 amod unaffected,members
R3894 T13331 T13329 compound family,members
R3895 T13332 T13282 punct .,used
R3896 T13334 T13335 det This,experiment
R3897 T13335 T13336 nsubjpass experiment,performed
R3898 T13337 T13336 auxpass was,performed
R3899 T13338 T13336 prep in,performed
R3900 T13339 T13340 det an,attempt
R3901 T13340 T13338 pobj attempt,in
R3902 T13341 T13342 aux to,amplify
R3903 T13342 T13340 acl amplify,attempt
R3904 T13343 T13342 prep across,amplify
R3905 T13344 T13345 det the,fragment
R3906 T13345 T13343 pobj fragment,across
R3907 T13346 T13345 amod deleted,fragment
R3908 T13347 T13342 cc and,amplify
R3909 T13348 T13342 conj define,amplify
R3910 T13349 T13350 det the,breakpoint
R3911 T13350 T13348 dobj breakpoint,define
R3912 T13351 T13350 amod exact,breakpoint
R3913 T13352 T13336 punct .,performed
R3914 T13354 T13355 det A,fragment
R3915 T13355 T13357 nsubjpass fragment,obtained
R3916 T13356 T13355 amod single,fragment
R3917 T13358 T13357 auxpass was,obtained
R3918 T13359 T13357 prep from,obtained
R3919 T13360 T13361 det the,primer
R3920 T13361 T13359 pobj primer,from
R3921 T13362 T13361 amod third,primer
R3922 T13363 T13361 amod forward,primer
R3923 T13364 T13361 prep from,primer
R3924 T13365 T13366 det the,side
R3925 T13366 T13364 pobj side,from
R3926 T13367 T13366 amod telomeric,side
R3927 T13368 T13369 punct (,TGAATGCTCAATTTTCCAGC
R3928 T13369 T13361 parataxis TGAATGCTCAATTTTCCAGC,primer
R3929 T13370 T13369 nmod T3f,TGAATGCTCAATTTTCCAGC
R3930 T13371 T13369 nummod 5,TGAATGCTCAATTTTCCAGC
R3931 T13372 T13371 punct ′,5
R3932 T13373 T13369 punct -,TGAATGCTCAATTTTCCAGC
R3933 T13374 T13369 punct -,TGAATGCTCAATTTTCCAGC
R3934 T13375 T13369 nummod 3,TGAATGCTCAATTTTCCAGC
R3935 T13376 T13369 punct ′,TGAATGCTCAATTTTCCAGC
R3936 T13377 T13369 punct ),TGAATGCTCAATTTTCCAGC
R3937 T13378 T13357 prep with,obtained
R3938 T13379 T13380 det the,primer
R3939 T13380 T13378 pobj primer,with
R3940 T13381 T13380 amod 11th,primer
R3941 T13382 T13380 amod reverse,primer
R3942 T13383 T13380 prep from,primer
R3943 T13384 T13385 det the,side
R3944 T13385 T13383 pobj side,from
R3945 T13386 T13385 amod centromeric,side
R3946 T13387 T13388 punct (,GGGAAAATGGATAGAGGGTG
R3947 T13388 T13380 parataxis GGGAAAATGGATAGAGGGTG,primer
R3948 T13389 T13388 nmod C11r,GGGAAAATGGATAGAGGGTG
R3949 T13390 T13388 nummod 5,GGGAAAATGGATAGAGGGTG
R3950 T13391 T13390 punct ′,5
R3951 T13392 T13388 punct -,GGGAAAATGGATAGAGGGTG
R3952 T13393 T13388 punct -,GGGAAAATGGATAGAGGGTG
R3953 T13394 T13388 nummod 3,GGGAAAATGGATAGAGGGTG
R3954 T13395 T13388 punct ′,GGGAAAATGGATAGAGGGTG
R3955 T13396 T13388 punct ),GGGAAAATGGATAGAGGGTG
R3956 T13397 T13357 punct .,obtained
R3957 T13399 T13400 det The,fragment
R3958 T13400 T13401 nsubjpass fragment,sequenced
R3959 T13402 T13400 punct ", ",fragment
R3960 T13403 T13404 dep which,is
R3961 T13404 T13400 relcl is,fragment
R3962 T13405 T13406 nummod 953,bp
R3963 T13406 T13404 attr bp,is
R3964 T13407 T13406 prep in,bp
R3965 T13408 T13407 pobj size,in
R3966 T13409 T13401 punct ", ",sequenced
R3967 T13410 T13401 auxpass was,sequenced
R3968 T13411 T13412 mark as,described
R3969 T13412 T13401 advcl described,sequenced
R3970 T13413 T13412 advmod above,described
R3971 T13414 T13412 cc and,described
R3972 T13415 T13412 conj compared,described
R3973 T13416 T13415 prep to,compared
R3974 T13417 T13418 det the,build
R3975 T13418 T13416 pobj build,to
R3976 T13419 T13418 amod current,build
R3977 T13420 T13418 prep of,build
R3978 T13421 T13422 det the,genome
R3979 T13422 T13420 pobj genome,of
R3980 T13423 T13422 amod human,genome
R3981 T13424 T13401 punct .,sequenced
R3982 T13426 T13427 det A,series
R3983 T13427 T13429 nsubjpass series,performed
R3984 T13428 T13427 amod similar,series
R3985 T13429 T13433 ccomp performed,were
R3986 T13430 T13427 prep of,series
R3987 T13431 T13430 pobj experiments,of
R3988 T13432 T13429 auxpass was,performed
R3989 T13434 T13435 aux to,identify
R3990 T13435 T13429 advcl identify,performed
R3991 T13436 T13437 det the,breakpoints
R3992 T13437 T13435 dobj breakpoints,identify
R3993 T13438 T13437 compound deletion,breakpoints
R3994 T13439 T13437 prep in,breakpoints
R3995 T13440 T13441 compound families,H27
R3996 T13441 T13439 pobj H27,in
R3997 T13442 T13441 cc and,H27
R3998 T13443 T13441 conj H33,H27
R3999 T13444 T13433 punct ;,were
R4000 T13445 T13433 nsubj we,were
R4001 T13446 T13433 acomp able,were
R4002 T13447 T13448 aux to,amplify
R4003 T13448 T13446 xcomp amplify,able
R4004 T13449 T13450 det a,product
R4005 T13450 T13448 dobj product,amplify
R4006 T13451 T13452 nummod 369,bp
R4007 T13452 T13450 compound bp,product
R4008 T13453 T13452 punct -,bp
R4009 T13454 T13450 compound PCR,product
R4010 T13455 T13448 prep across,amplify
R4011 T13456 T13457 det the,breakpoint
R4012 T13457 T13455 pobj breakpoint,across
R4013 T13458 T13457 acl found,breakpoint
R4014 T13459 T13458 prep in,found
R4015 T13460 T13461 amod affected,members
R4016 T13461 T13459 pobj members,in
R4017 T13462 T13461 prep of,members
R4018 T13463 T13464 compound family,H27
R4019 T13464 T13462 pobj H27,of
R4020 T13465 T13448 advcl using,amplify
R4021 T13466 T13467 compound primer,pair
R4022 T13467 T13465 dobj pair,using
R4023 T13468 T13469 nmod H27,11F
R4024 T13469 T13471 nmod 11F,GACCTCAAGAAGGCATGAATAC
R4025 T13470 T13469 punct -,11F
R4026 T13471 T13467 appos GACCTCAAGAAGGCATGAATAC,pair
R4027 T13472 T13471 nummod 5,GACCTCAAGAAGGCATGAATAC
R4028 T13473 T13472 punct ′,5
R4029 T13474 T13471 punct -,GACCTCAAGAAGGCATGAATAC
R4030 T13475 T13471 punct -,GACCTCAAGAAGGCATGAATAC
R4031 T13476 T13471 nummod 3,GACCTCAAGAAGGCATGAATAC
R4032 T13477 T13471 punct ′,GACCTCAAGAAGGCATGAATAC
R4033 T13478 T13471 cc and,GACCTCAAGAAGGCATGAATAC
R4034 T13479 T13480 nmod H27,3R
R4035 T13480 T13482 nmod 3R,ATGGTGGCCAGGTACACAAG
R4036 T13481 T13480 punct -,3R
R4037 T13482 T13471 conj ATGGTGGCCAGGTACACAAG,GACCTCAAGAAGGCATGAATAC
R4038 T13483 T13482 nummod 5,ATGGTGGCCAGGTACACAAG
R4039 T13484 T13483 punct ′,5
R4040 T13485 T13482 punct -,ATGGTGGCCAGGTACACAAG
R4041 T13486 T13482 punct -,ATGGTGGCCAGGTACACAAG
R4042 T13487 T13482 nummod 3,ATGGTGGCCAGGTACACAAG
R4043 T13488 T13482 punct ′,ATGGTGGCCAGGTACACAAG
R4044 T13489 T13490 punct (,S4
R4045 T13490 T13465 parataxis S4,using
R4046 T13491 T13490 compound Figure,S4
R4047 T13492 T13490 punct ),S4
R4048 T13493 T13433 punct ", ",were
R4049 T13494 T13433 cc but,were
R4050 T13495 T13496 prep to,been
R4051 T13496 T13433 conj been,were
R4052 T13497 T13495 pobj date,to
R4053 T13498 T13496 nsubj we,been
R4054 T13499 T13496 aux have,been
R4055 T13500 T13496 acomp unable,been
R4056 T13501 T13502 aux to,identify
R4057 T13502 T13500 xcomp identify,unable
R4058 T13503 T13504 det the,breakpoint
R4059 T13504 T13502 dobj breakpoint,identify
R4060 T13505 T13504 prep in,breakpoint
R4061 T13506 T13507 compound family,H33
R4062 T13507 T13505 pobj H33,in
R4063 T13508 T13433 punct .,were
R4064 T13807 T13808 compound Western,blot
R4065 T13808 T13809 compound blot,analysis
R4066 T13810 T13809 prep in,analysis
R4067 T13811 T13812 compound SCA15,patients
R4068 T13812 T13810 pobj patients,in
R4069 T13813 T13809 punct .,analysis
R4070 T13815 T13816 nmod EBV,lymphoblasts
R4071 T13816 T13818 nsubjpass lymphoblasts,used
R4072 T13817 T13816 amod immortalized,lymphoblasts
R4073 T13818 T13836 ccomp used,came
R4074 T13819 T13816 prep from,lymphoblasts
R4075 T13820 T13821 nummod three,members
R4076 T13821 T13819 pobj members,from
R4077 T13822 T13821 amod affected,members
R4078 T13823 T13821 compound family,members
R4079 T13824 T13825 dep who,carry
R4080 T13825 T13821 relcl carry,members
R4081 T13826 T13827 det the,deletion
R4082 T13827 T13825 dobj deletion,carry
R4083 T13828 T13821 cc and,members
R4084 T13829 T13830 nummod one,member
R4085 T13830 T13821 conj member,members
R4086 T13831 T13830 compound family,member
R4087 T13832 T13830 prep without,member
R4088 T13833 T13834 det the,mutation
R4089 T13834 T13832 pobj mutation,without
R4090 T13835 T13818 auxpass were,used
R4091 T13837 T13818 prep as,used
R4092 T13838 T13839 det a,source
R4093 T13839 T13837 pobj source,as
R4094 T13840 T13841 advmod readily,accessible
R4095 T13841 T13839 amod accessible,source
R4096 T13842 T13839 prep of,source
R4097 T13843 T13842 pobj protein,of
R4098 T13844 T13836 punct ;,came
R4099 T13845 T13846 det all,samples
R4100 T13846 T13836 nsubj samples,came
R4101 T13847 T13836 prep from,came
R4102 T13848 T13847 pobj members,from
R4103 T13849 T13848 prep of,members
R4104 T13850 T13851 compound family,AUS1
R4105 T13851 T13849 pobj AUS1,of
R4106 T13852 T13836 punct .,came
R4107 T13854 T13855 compound Protein,extraction
R4108 T13855 T13856 nsubjpass extraction,performed
R4109 T13857 T13856 auxpass was,performed
R4110 T13858 T13856 advcl using,performed
R4111 T13859 T13860 compound lysis,buffer
R4112 T13860 T13858 dobj buffer,using
R4113 T13861 T13860 acl containing,buffer
R4114 T13862 T13863 nummod 1,TBS
R4115 T13863 T13861 dobj TBS,containing
R4116 T13864 T13862 punct ×,1
R4117 T13865 T13863 punct ", ",TBS
R4118 T13866 T13867 nummod 1,%
R4119 T13867 T13868 compound %,X
R4120 T13868 T13863 conj X,TBS
R4121 T13869 T13868 compound Triton,X
R4122 T13870 T13868 punct -,X
R4123 T13871 T13868 nummod 100,X
R4124 T13872 T13868 punct ", ",X
R4125 T13873 T13868 cc and,X
R4126 T13874 T13875 det a,cocktail
R4127 T13875 T13868 conj cocktail,X
R4128 T13876 T13875 prep of,cocktail
R4129 T13877 T13878 compound protease,inhibitors
R4130 T13878 T13876 pobj inhibitors,of
R4131 T13879 T13880 punct (,Roche
R4132 T13880 T13878 parataxis Roche,inhibitors
R4133 T13881 T13880 punct ),Roche
R4134 T13882 T13856 prep with,performed
R4135 T13883 T13884 amod overnight,lysis
R4136 T13884 T13882 pobj lysis,with
R4137 T13885 T13856 prep at,performed
R4138 T13886 T13887 punct −,80
R4139 T13887 T13888 nummod 80,°C
R4140 T13888 T13885 pobj °C,at
R4141 T13889 T13856 punct .,performed
R4142 T13891 T13892 nsubjpass Homogenates,diluted
R4143 T13893 T13892 auxpass were,diluted
R4144 T13894 T13892 advmod appropriately,diluted
R4145 T13895 T13892 punct ", ",diluted
R4146 T13896 T13892 conj mixed,diluted
R4147 T13897 T13896 prep with,mixed
R4148 T13898 T13899 nummod 4,buffer
R4149 T13899 T13897 pobj buffer,with
R4150 T13900 T13898 punct ×,4
R4151 T13901 T13899 amod reducing,buffer
R4152 T13902 T13899 compound sample,buffer
R4153 T13903 T13896 punct ", ",mixed
R4154 T13904 T13896 cc and,mixed
R4155 T13905 T13896 conj loaded,mixed
R4156 T13906 T13905 prep onto,loaded
R4157 T13907 T13908 nummod 4,%
R4158 T13908 T13909 nmod %,gels
R4159 T13909 T13906 pobj gels,onto
R4160 T13910 T13911 punct –,%
R4161 T13911 T13908 prep %,%
R4162 T13912 T13911 nummod 12,%
R4163 T13913 T13909 amod precast,gels
R4164 T13914 T13909 compound gradient,gels
R4165 T13915 T13916 punct (,NuPAGE
R4166 T13916 T13909 parataxis NuPAGE,gels
R4167 T13917 T13916 punct ", ",NuPAGE
R4168 T13918 T13916 appos Invitrogen,NuPAGE
R4169 T13919 T13916 punct ),NuPAGE
R4170 T13920 T13905 prep for,loaded
R4171 T13921 T13922 compound SDS,PAGE
R4172 T13922 T13920 pobj PAGE,for
R4173 T13923 T13922 punct -,PAGE
R4174 T13924 T13922 cc and,PAGE
R4175 T13925 T13922 conj immunoblotting,PAGE
R4176 T13926 T13892 punct .,diluted
R4177 T13928 T13929 det The,antibodies
R4178 T13929 T13930 nsubjpass antibodies,used
R4179 T13931 T13929 prep to,antibodies
R4180 T13932 T13931 pobj ITPR1,to
R4181 T13933 T13934 punct (,1
R4182 T13934 T13932 parataxis 1,ITPR1
R4183 T13935 T13936 punct :,"1,000"
R4184 T13936 T13934 prep "1,000",1
R4185 T13937 T13934 punct ),1
R4186 T13938 T13932 cc and,ITPR1
R4187 T13939 T13932 conj ACTB,ITPR1
R4188 T13940 T13941 punct (,1
R4189 T13941 T13939 parataxis 1,ACTB
R4190 T13942 T13943 punct :,"5,000"
R4191 T13943 T13941 prep "5,000",1
R4192 T13944 T13941 punct ),1
R4193 T13945 T13930 auxpass were,used
R4194 T13946 T13947 mark as,recommended
R4195 T13947 T13930 advcl recommended,used
R4196 T13948 T13947 agent by,recommended
R4197 T13949 T13948 pobj manufacturers,by
R4198 T13950 T13930 punct .,used

2_test

Id Subject Object Predicate Lexical cue
17590087-10742102-85657859 253-255 10742102 denotes 12
17590087-6587361-85657860 483-485 6587361 denotes 13
17590087-12112377-85657861 4059-4061 12112377 denotes 14
T7451 253-255 10742102 denotes 12
T31814 483-485 6587361 denotes 13
T31713 4059-4061 12112377 denotes 14