Id |
Subject |
Object |
Predicate |
Lexical cue |
T8122 |
0-802 |
sentence |
denotes |
Figure 4 Western Blot Analysis of ITPR1 Protein Levels in EBV Immortalized Lymphoblasts from AUS1 Family Members
Western blot performed to examine ITPR1 levels in EBV immortalized lymphocytes from AUS1 affected family members carrying the ITPR1 deletion and from an AUS1 family member of unknown disease status who does not carry the deletion. Notably the samples from patients with ITPR1 deletion show a dramatic decrease in ITPR1 levels. To demonstrate equal loading, these samples were diluted one in five, and the Western blot was repeated using an antibody against ACTB. Itpr1 contains three domains, an N-terminal inositol triphosphate binding domain, a coupling domain, and a C-terminal transmembrane domain; it also contains two protein kinase A phosphorylation sites and an ATP-binding site. |
T8123 |
578-583 |
NN |
denotes |
Itpr1 |
T8124 |
584-592 |
VBZ |
denotes |
contains |
T8125 |
726-734 |
VBZ |
denotes |
contains |
T8126 |
593-598 |
CD |
denotes |
three |
T8127 |
599-606 |
NNS |
denotes |
domains |
T8128 |
606-608 |
, |
denotes |
, |
T8129 |
608-610 |
DT |
denotes |
an |
T8130 |
652-658 |
NN |
denotes |
domain |
T8131 |
611-612 |
NN |
denotes |
N |
T8132 |
613-621 |
JJ |
denotes |
terminal |
T8133 |
612-613 |
HYPH |
denotes |
- |
T8134 |
622-630 |
NN |
denotes |
inositol |
T8135 |
631-643 |
NN |
denotes |
triphosphate |
T8136 |
644-651 |
NN |
denotes |
binding |
T8137 |
658-660 |
, |
denotes |
, |
T8138 |
660-661 |
DT |
denotes |
a |
T8139 |
671-677 |
NN |
denotes |
domain |
T8140 |
662-670 |
VBG |
denotes |
coupling |
T8141 |
677-679 |
, |
denotes |
, |
T8142 |
679-682 |
CC |
denotes |
and |
T8143 |
683-684 |
DT |
denotes |
a |
T8144 |
710-716 |
NN |
denotes |
domain |
T8145 |
685-686 |
NN |
denotes |
C |
T8146 |
687-695 |
JJ |
denotes |
terminal |
T8147 |
686-687 |
HYPH |
denotes |
- |
T8148 |
696-709 |
NN |
denotes |
transmembrane |
T8149 |
716-717 |
: |
denotes |
; |
T8150 |
718-720 |
PRP |
denotes |
it |
T8151 |
721-725 |
RB |
denotes |
also |
T8152 |
735-738 |
CD |
denotes |
two |
T8153 |
772-777 |
NNS |
denotes |
sites |
T8154 |
739-746 |
NN |
denotes |
protein |
T8155 |
754-755 |
NN |
denotes |
A |
T8156 |
747-753 |
NN |
denotes |
kinase |
T8157 |
756-771 |
NN |
denotes |
phosphorylation |
T8158 |
778-781 |
CC |
denotes |
and |
T8159 |
782-784 |
DT |
denotes |
an |
T8160 |
797-801 |
NN |
denotes |
site |
T8161 |
785-788 |
NN |
denotes |
ATP |
T8162 |
789-796 |
VBG |
denotes |
binding |
T8163 |
788-789 |
HYPH |
denotes |
- |
T8164 |
801-802 |
. |
denotes |
. |
T16337 |
555-563 |
NN |
denotes |
antibody |
T16338 |
564-571 |
IN |
denotes |
against |
T16245 |
11-18 |
NNP |
denotes |
Western |
T16246 |
19-23 |
NN |
denotes |
Blot |
T16247 |
24-32 |
NN |
denotes |
Analysis |
T16248 |
33-35 |
IN |
denotes |
of |
T16249 |
36-41 |
NN |
denotes |
ITPR1 |
T16250 |
50-56 |
NNS |
denotes |
Levels |
T16251 |
42-49 |
NN |
denotes |
Protein |
T16252 |
57-59 |
IN |
denotes |
in |
T16253 |
60-63 |
NN |
denotes |
EBV |
T16254 |
77-89 |
NNS |
denotes |
Lymphoblasts |
T16255 |
64-76 |
VBN |
denotes |
Immortalized |
T16256 |
90-94 |
IN |
denotes |
from |
T16257 |
95-99 |
NN |
denotes |
AUS1 |
T16258 |
107-114 |
NNS |
denotes |
Members |
T16259 |
100-106 |
NN |
denotes |
Family |
T16260 |
114-345 |
sentence |
denotes |
Western blot performed to examine ITPR1 levels in EBV immortalized lymphocytes from AUS1 affected family members carrying the ITPR1 deletion and from an AUS1 family member of unknown disease status who does not carry the deletion. |
T16261 |
115-122 |
NNP |
denotes |
Western |
T16262 |
123-127 |
NN |
denotes |
blot |
T16263 |
128-137 |
VBN |
denotes |
performed |
T16264 |
138-140 |
TO |
denotes |
to |
T16265 |
141-148 |
VB |
denotes |
examine |
T16266 |
149-154 |
NN |
denotes |
ITPR1 |
T16267 |
155-161 |
NNS |
denotes |
levels |
T16268 |
162-164 |
IN |
denotes |
in |
T16269 |
165-168 |
NN |
denotes |
EBV |
T16270 |
182-193 |
NNS |
denotes |
lymphocytes |
T16271 |
169-181 |
VBN |
denotes |
immortalized |
T16272 |
194-198 |
IN |
denotes |
from |
T16273 |
199-203 |
NN |
denotes |
AUS1 |
T16274 |
204-212 |
VBN |
denotes |
affected |
T16275 |
220-227 |
NNS |
denotes |
members |
T16276 |
213-219 |
NN |
denotes |
family |
T16277 |
228-236 |
VBG |
denotes |
carrying |
T16278 |
237-240 |
DT |
denotes |
the |
T16279 |
247-255 |
NN |
denotes |
deletion |
T16280 |
241-246 |
NN |
denotes |
ITPR1 |
T16281 |
256-259 |
CC |
denotes |
and |
T16282 |
260-264 |
IN |
denotes |
from |
T16283 |
265-267 |
DT |
denotes |
an |
T16284 |
280-286 |
NN |
denotes |
member |
T16285 |
268-272 |
NN |
denotes |
AUS1 |
T16286 |
273-279 |
NN |
denotes |
family |
T16287 |
287-289 |
IN |
denotes |
of |
T16288 |
290-297 |
JJ |
denotes |
unknown |
T16289 |
306-312 |
NN |
denotes |
status |
T16290 |
298-305 |
NN |
denotes |
disease |
T16291 |
313-316 |
WP |
denotes |
who |
T16292 |
326-331 |
VB |
denotes |
carry |
T16293 |
317-321 |
VBZ |
denotes |
does |
T16294 |
322-325 |
RB |
denotes |
not |
T16295 |
332-335 |
DT |
denotes |
the |
T16296 |
336-344 |
NN |
denotes |
deletion |
T16297 |
344-345 |
. |
denotes |
. |
T16298 |
345-441 |
sentence |
denotes |
Notably the samples from patients with ITPR1 deletion show a dramatic decrease in ITPR1 levels. |
T16299 |
346-353 |
RB |
denotes |
Notably |
T16300 |
400-404 |
VBP |
denotes |
show |
T16301 |
354-357 |
DT |
denotes |
the |
T16302 |
358-365 |
NNS |
denotes |
samples |
T16303 |
366-370 |
IN |
denotes |
from |
T16304 |
371-379 |
NNS |
denotes |
patients |
T16305 |
380-384 |
IN |
denotes |
with |
T16306 |
385-390 |
NN |
denotes |
ITPR1 |
T16307 |
391-399 |
NN |
denotes |
deletion |
T16308 |
405-406 |
DT |
denotes |
a |
T16309 |
416-424 |
NN |
denotes |
decrease |
T16310 |
407-415 |
JJ |
denotes |
dramatic |
T16311 |
425-427 |
IN |
denotes |
in |
T16312 |
428-433 |
NN |
denotes |
ITPR1 |
T16313 |
434-440 |
NNS |
denotes |
levels |
T16314 |
440-441 |
. |
denotes |
. |
T16315 |
441-577 |
sentence |
denotes |
To demonstrate equal loading, these samples were diluted one in five, and the Western blot was repeated using an antibody against ACTB. |
T16316 |
442-444 |
TO |
denotes |
To |
T16317 |
445-456 |
VB |
denotes |
demonstrate |
T16318 |
491-498 |
VBN |
denotes |
diluted |
T16319 |
457-462 |
JJ |
denotes |
equal |
T16320 |
463-470 |
NN |
denotes |
loading |
T16321 |
470-472 |
, |
denotes |
, |
T16322 |
472-477 |
DT |
denotes |
these |
T16323 |
478-485 |
NNS |
denotes |
samples |
T16324 |
486-490 |
VBD |
denotes |
were |
T16325 |
499-502 |
CD |
denotes |
one |
T16326 |
503-505 |
IN |
denotes |
in |
T16327 |
506-510 |
CD |
denotes |
five |
T16328 |
510-512 |
, |
denotes |
, |
T16329 |
512-515 |
CC |
denotes |
and |
T16330 |
516-519 |
DT |
denotes |
the |
T16331 |
528-532 |
NN |
denotes |
blot |
T16332 |
520-527 |
NNP |
denotes |
Western |
T16333 |
537-545 |
VBN |
denotes |
repeated |
T16334 |
533-536 |
VBD |
denotes |
was |
T16335 |
546-551 |
VBG |
denotes |
using |
T16336 |
552-554 |
DT |
denotes |
an |
T16339 |
572-576 |
NN |
denotes |
ACTB |
T16340 |
576-577 |
. |
denotes |
. |
R2029 |
T8123 |
T8124 |
nsubj |
Itpr1,contains |
R2030 |
T8124 |
T8125 |
ccomp |
contains,contains |
R2032 |
T8126 |
T8127 |
nummod |
three,domains |
R2033 |
T8127 |
T8124 |
dobj |
domains,contains |
R2035 |
T8128 |
T8127 |
punct |
", ",domains |
R2037 |
T8129 |
T8130 |
det |
an,domain |
R2039 |
T8130 |
T8127 |
appos |
domain,domains |
R2040 |
T8131 |
T8132 |
npadvmod |
N,terminal |
R2041 |
T8132 |
T8130 |
amod |
terminal,domain |
R2043 |
T8133 |
T8132 |
punct |
-,terminal |
R2044 |
T8134 |
T8130 |
compound |
inositol,domain |
R2045 |
T8135 |
T8130 |
compound |
triphosphate,domain |
R2047 |
T8136 |
T8130 |
compound |
binding,domain |
R2048 |
T8137 |
T8130 |
punct |
", ",domain |
R2050 |
T8138 |
T8139 |
det |
a,domain |
R2051 |
T8139 |
T8130 |
conj |
domain,domain |
R2052 |
T8140 |
T8139 |
amod |
coupling,domain |
R2054 |
T8141 |
T8139 |
punct |
", ",domain |
R2055 |
T8142 |
T8139 |
cc |
and,domain |
R2057 |
T8143 |
T8144 |
det |
a,domain |
R2058 |
T8144 |
T8139 |
conj |
domain,domain |
R2059 |
T8145 |
T8146 |
npadvmod |
C,terminal |
R2061 |
T8146 |
T8144 |
amod |
terminal,domain |
R2062 |
T8147 |
T8146 |
punct |
-,terminal |
R2063 |
T8148 |
T8144 |
compound |
transmembrane,domain |
R2064 |
T8149 |
T8125 |
punct |
;,contains |
R2065 |
T8150 |
T8125 |
nsubj |
it,contains |
R2067 |
T8151 |
T8125 |
advmod |
also,contains |
R2068 |
T8152 |
T8153 |
nummod |
two,sites |
R2069 |
T8153 |
T8125 |
dobj |
sites,contains |
R2071 |
T8154 |
T8155 |
compound |
protein,A |
R2072 |
T8155 |
T8153 |
compound |
A,sites |
R2073 |
T8156 |
T8155 |
compound |
kinase,A |
R2075 |
T8157 |
T8153 |
compound |
phosphorylation,sites |
R2076 |
T8158 |
T8153 |
cc |
and,sites |
R2077 |
T8159 |
T8160 |
det |
an,site |
R2079 |
T8160 |
T8153 |
conj |
site,sites |
R2080 |
T8161 |
T8162 |
npadvmod |
ATP,binding |
R2081 |
T8162 |
T8160 |
amod |
binding,site |
R2083 |
T8163 |
T8162 |
punct |
-,binding |
R2084 |
T8164 |
T8125 |
punct |
.,contains |
R4848 |
T16245 |
T16246 |
compound |
Western,Blot |
R4849 |
T16246 |
T16247 |
compound |
Blot,Analysis |
R4850 |
T16248 |
T16247 |
prep |
of,Analysis |
R4851 |
T16249 |
T16250 |
compound |
ITPR1,Levels |
R4852 |
T16250 |
T16248 |
pobj |
Levels,of |
R4853 |
T16251 |
T16250 |
compound |
Protein,Levels |
R4854 |
T16252 |
T16247 |
prep |
in,Analysis |
R4855 |
T16253 |
T16254 |
nmod |
EBV,Lymphoblasts |
R4856 |
T16254 |
T16252 |
pobj |
Lymphoblasts,in |
R4857 |
T16255 |
T16254 |
amod |
Immortalized,Lymphoblasts |
R4858 |
T16256 |
T16254 |
prep |
from,Lymphoblasts |
R4859 |
T16257 |
T16258 |
compound |
AUS1,Members |
R4860 |
T16258 |
T16256 |
pobj |
Members,from |
R4861 |
T16259 |
T16258 |
compound |
Family,Members |
R4862 |
T16261 |
T16262 |
compound |
Western,blot |
R4863 |
T16263 |
T16262 |
acl |
performed,blot |
R4864 |
T16264 |
T16265 |
aux |
to,examine |
R4865 |
T16265 |
T16263 |
advcl |
examine,performed |
R4866 |
T16266 |
T16267 |
compound |
ITPR1,levels |
R4867 |
T16267 |
T16265 |
dobj |
levels,examine |
R4868 |
T16268 |
T16267 |
prep |
in,levels |
R4869 |
T16269 |
T16270 |
nmod |
EBV,lymphocytes |
R4870 |
T16270 |
T16268 |
pobj |
lymphocytes,in |
R4871 |
T16271 |
T16270 |
amod |
immortalized,lymphocytes |
R4872 |
T16272 |
T16270 |
prep |
from,lymphocytes |
R4873 |
T16273 |
T16274 |
npadvmod |
AUS1,affected |
R4874 |
T16274 |
T16275 |
amod |
affected,members |
R4875 |
T16275 |
T16272 |
pobj |
members,from |
R4876 |
T16276 |
T16275 |
compound |
family,members |
R4877 |
T16277 |
T16275 |
acl |
carrying,members |
R4878 |
T16278 |
T16279 |
det |
the,deletion |
R4879 |
T16279 |
T16277 |
dobj |
deletion,carrying |
R4880 |
T16280 |
T16279 |
compound |
ITPR1,deletion |
R4881 |
T16281 |
T16272 |
cc |
and,from |
R4882 |
T16282 |
T16272 |
conj |
from,from |
R4883 |
T16283 |
T16284 |
det |
an,member |
R4884 |
T16284 |
T16282 |
pobj |
member,from |
R4885 |
T16285 |
T16284 |
compound |
AUS1,member |
R4886 |
T16286 |
T16284 |
compound |
family,member |
R4887 |
T16287 |
T16284 |
prep |
of,member |
R4888 |
T16288 |
T16289 |
amod |
unknown,status |
R4889 |
T16289 |
T16287 |
pobj |
status,of |
R4890 |
T16290 |
T16289 |
compound |
disease,status |
R4891 |
T16291 |
T16292 |
dep |
who,carry |
R4892 |
T16292 |
T16284 |
relcl |
carry,member |
R4893 |
T16293 |
T16292 |
aux |
does,carry |
R4894 |
T16294 |
T16292 |
neg |
not,carry |
R4895 |
T16295 |
T16296 |
det |
the,deletion |
R4896 |
T16296 |
T16292 |
dobj |
deletion,carry |
R4897 |
T16297 |
T16262 |
punct |
.,blot |
R4898 |
T16299 |
T16300 |
advmod |
Notably,show |
R4899 |
T16301 |
T16302 |
det |
the,samples |
R4900 |
T16302 |
T16300 |
nsubj |
samples,show |
R4901 |
T16303 |
T16302 |
prep |
from,samples |
R4902 |
T16304 |
T16303 |
pobj |
patients,from |
R4903 |
T16305 |
T16304 |
prep |
with,patients |
R4904 |
T16306 |
T16307 |
compound |
ITPR1,deletion |
R4905 |
T16307 |
T16305 |
pobj |
deletion,with |
R4906 |
T16308 |
T16309 |
det |
a,decrease |
R4907 |
T16309 |
T16300 |
dobj |
decrease,show |
R4908 |
T16310 |
T16309 |
amod |
dramatic,decrease |
R4909 |
T16311 |
T16309 |
prep |
in,decrease |
R4910 |
T16312 |
T16313 |
compound |
ITPR1,levels |
R4911 |
T16313 |
T16311 |
pobj |
levels,in |
R4912 |
T16314 |
T16300 |
punct |
.,show |
R4913 |
T16316 |
T16317 |
aux |
To,demonstrate |
R4914 |
T16317 |
T16318 |
advcl |
demonstrate,diluted |
R4915 |
T16319 |
T16320 |
amod |
equal,loading |
R4916 |
T16320 |
T16317 |
dobj |
loading,demonstrate |
R4917 |
T16321 |
T16318 |
punct |
", ",diluted |
R4918 |
T16322 |
T16323 |
det |
these,samples |
R4919 |
T16323 |
T16318 |
nsubjpass |
samples,diluted |
R4920 |
T16324 |
T16318 |
auxpass |
were,diluted |
R4921 |
T16325 |
T16318 |
npadvmod |
one,diluted |
R4922 |
T16326 |
T16325 |
prep |
in,one |
R4923 |
T16327 |
T16326 |
pobj |
five,in |
R4924 |
T16328 |
T16318 |
punct |
", ",diluted |
R4925 |
T16329 |
T16318 |
cc |
and,diluted |
R4926 |
T16330 |
T16331 |
det |
the,blot |
R4927 |
T16331 |
T16333 |
nsubjpass |
blot,repeated |
R4928 |
T16332 |
T16331 |
compound |
Western,blot |
R4929 |
T16333 |
T16318 |
conj |
repeated,diluted |
R4930 |
T16334 |
T16333 |
auxpass |
was,repeated |
R4931 |
T16335 |
T16333 |
advcl |
using,repeated |
R4932 |
T16336 |
T16337 |
det |
an,antibody |
R4933 |
T16337 |
T16335 |
dobj |
antibody,using |
R4934 |
T16338 |
T16337 |
prep |
against,antibody |
R4935 |
T16339 |
T16338 |
pobj |
ACTB,against |
R4936 |
T16340 |
T16318 |
punct |
.,diluted |