PMC:1885650 / 9958-10849 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1885650","sourcedb":"PMC","sourceid":"1885650","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1885650","text":"Figure 3. The upper panel shows a Southern blot DHS map of the human STIL promoter in K562 cells using a 3′ probe (grey box labelled ‘P’) to analyse the BglII-restricted fragment (cut −4 kb and +4.2 kb relative to the STIL transcription start site). This revealed a core DHS ∼500 bp wide. The lower panel represents the mean ± SD real-time PCR quantification of enrichments from three biological replicates of K562 material relative to known genomic standards, using 10 separate primer sets that together amplify ∼1200 bp centred around the STIL transcription start site. The black bar represents the approximate location of the DHS, as identified by the Southern blot experiment in the upper panel. The real-time PCR amplification profile closely represents the Southern blot profile, although a central dip in real-time PCR signal is observed over the site of transcription factor binding.","tracks":[]}