PMC:1885650 / 5748-6569 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1885650","sourcedb":"PMC","sourceid":"1885650","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1885650","text":"Figure 2. (A) The left-hand panels show the Sybr-green real-time PCR profiles (fluorescence versus cycle number) of 5-fold dilutions of quantified mouse genomic DNA standards for two primer pairs within (ppA) and 3′ (ppB) of the Stil promoter. The right-hand panel shows that DNA template derived from the 40 units DNaseI-treated sample amplified nearly six PCR cycles in advance of the 0 DNaseI-treated sample with ppA. No difference in amplification kinetics was seen between the 0 and 40 samples with ppB. (B) Quantification of samples relative to genomic standards using primers for the ‘housekeeping’ Hmbs promoter shows maximal enrichment with 40 units DNaseI treatment for primary mouse thymocytes (left panel) compared with maximal enrichment with 120 units DNaseI for the mouse T-cell line, BW5147 (right panel).","tracks":[]}