Id |
Subject |
Object |
Predicate |
Lexical cue |
T22075 |
1315-1319 |
NN |
denotes |
Actb |
T22074 |
1314-1315 |
-LRB- |
denotes |
( |
T22073 |
1293-1305 |
NN |
denotes |
housekeeping |
T22072 |
1284-1292 |
JJ |
denotes |
internal |
T22071 |
1306-1313 |
NN |
denotes |
control |
T22070 |
1281-1283 |
DT |
denotes |
an |
T22069 |
1278-1280 |
IN |
denotes |
to |
T22068 |
1262-1266 |
VBD |
denotes |
were |
T22067 |
1267-1277 |
VBN |
denotes |
normalized |
T22066 |
1257-1261 |
NNS |
denotes |
data |
T22065 |
1253-1256 |
DT |
denotes |
All |
T22064 |
1252-1377 |
sentence |
denotes |
All data were normalized to an internal housekeeping control (Actb) and analyzed using the 2(-Delta Delta C(T)) method [38]. |
T22063 |
1251-1252 |
. |
denotes |
. |
T22062 |
1243-1251 |
NN |
denotes |
amplicon |
T22061 |
1239-1242 |
DT |
denotes |
the |
T22060 |
1236-1238 |
IN |
denotes |
of |
T22059 |
1224-1235 |
NN |
denotes |
specificity |
T22058 |
1217-1223 |
VB |
denotes |
insure |
T21924 |
363-371 |
NNP |
denotes |
Carlsbad |
T21923 |
361-363 |
, |
denotes |
, |
T21922 |
351-361 |
NNP |
denotes |
Invitrogen |
T21921 |
350-351 |
-LRB- |
denotes |
( |
T21920 |
340-349 |
NNS |
denotes |
protocols |
T21919 |
327-339 |
JJ |
denotes |
conventional |
T21918 |
324-326 |
IN |
denotes |
to |
T21917 |
314-323 |
VBG |
denotes |
according |
T21916 |
305-313 |
NNS |
denotes |
hexamers |
T21915 |
298-304 |
JJ |
denotes |
random |
T21914 |
292-297 |
VBG |
denotes |
using |
T21913 |
278-281 |
VBD |
denotes |
was |
T21912 |
282-291 |
VBN |
denotes |
generated |
T21911 |
273-277 |
NN |
denotes |
cDNA |
T21910 |
272-382 |
sentence |
denotes |
cDNA was generated using random hexamers according to conventional protocols (Invitrogen, Carlsbad, CA, USA). |
T21909 |
271-272 |
. |
denotes |
. |
T21908 |
262-271 |
NN |
denotes |
treatment |
T21907 |
255-261 |
NN |
denotes |
DNAse1 |
T21906 |
245-254 |
VBG |
denotes |
including |
T21905 |
243-244 |
-RRB- |
denotes |
) |
T21904 |
240-243 |
NNP |
denotes |
USA |
T21903 |
237-239 |
NNP |
denotes |
CA |
T21902 |
235-237 |
, |
denotes |
, |
T21901 |
230-235 |
NNP |
denotes |
Jolla |
T21900 |
227-229 |
NNP |
denotes |
La |
T21899 |
225-227 |
, |
denotes |
, |
T21898 |
215-225 |
NNP |
denotes |
Stratagene |
T21897 |
213-214 |
: |
denotes |
; |
T21896 |
200-209 |
NN |
denotes |
Microprep |
T21895 |
196-199 |
NNP |
denotes |
RNA |
T21894 |
210-213 |
NN |
denotes |
Kit |
T21893 |
185-195 |
NNP |
denotes |
Absolutely |
T21892 |
184-185 |
-LRB- |
denotes |
( |
T21891 |
169-179 |
JJ |
denotes |
commercial |
T21890 |
180-183 |
NN |
denotes |
kit |
T21889 |
167-168 |
DT |
denotes |
a |
T21888 |
161-166 |
VBG |
denotes |
using |
T21887 |
148-151 |
VBD |
denotes |
was |
T21886 |
146-147 |
-RRB- |
denotes |
) |
T21885 |
138-146 |
NN |
denotes |
analysis |
T21884 |
128-137 |
NN |
denotes |
microrray |
T21883 |
124-127 |
IN |
denotes |
for |
T21882 |
119-123 |
VBN |
denotes |
used |
T21881 |
111-118 |
NNS |
denotes |
kidneys |
T21880 |
107-110 |
DT |
denotes |
the |
T21879 |
102-106 |
IN |
denotes |
from |
T21878 |
93-101 |
JJ |
denotes |
distinct |
T21877 |
89-92 |
CC |
denotes |
and |
T21876 |
79-88 |
NN |
denotes |
duplicate |
T21875 |
76-78 |
IN |
denotes |
in |
T21874 |
64-75 |
VBN |
denotes |
represented |
T21873 |
59-63 |
CC |
denotes |
both |
T21872 |
58-59 |
-LRB- |
denotes |
( |
T21871 |
42-49 |
NN |
denotes |
control |
T21870 |
38-41 |
CC |
denotes |
and |
T21869 |
33-37 |
JJ |
denotes |
null |
T21868 |
26-27 |
HYPH |
denotes |
/ |
T21867 |
27-32 |
NN |
denotes |
Pygo2 |
T21866 |
21-26 |
NN |
denotes |
Pygo1 |
T22057 |
1214-1216 |
TO |
denotes |
to |
T22056 |
1204-1208 |
VBD |
denotes |
were |
T22055 |
1200-1203 |
VBN |
denotes |
set |
T22054 |
1193-1199 |
NN |
denotes |
primer |
T22053 |
1188-1192 |
DT |
denotes |
each |
T22052 |
1185-1187 |
IN |
denotes |
of |
T22051 |
1209-1213 |
VBN |
denotes |
used |
T22050 |
1171-1172 |
HYPH |
denotes |
- |
T22049 |
1172-1175 |
NN |
denotes |
gel |
T22048 |
1164-1171 |
NN |
denotes |
agarose |
T22047 |
1160-1163 |
CC |
denotes |
and |
T22046 |
1176-1184 |
NN |
denotes |
analysis |
T22045 |
1154-1159 |
NN |
denotes |
curve |
T22044 |
1141-1153 |
NN |
denotes |
Dissociation |
T22043 |
1140-1252 |
sentence |
denotes |
Dissociation curve and agarose-gel analysis of each primer set were used to insure specificity of the amplicon. |
T22042 |
1139-1140 |
. |
denotes |
. |
T22041 |
1138-1139 |
-RRB- |
denotes |
) |
T22040 |
1126-1127 |
: |
denotes |
; |
T22039 |
1119-1126 |
NN |
denotes |
Mx3000p |
T22038 |
1128-1138 |
NNP |
denotes |
Stratagene |
T22037 |
1118-1119 |
-LRB- |
denotes |
( |
T22036 |
1107-1110 |
NN |
denotes |
PCR |
T22035 |
1094-1106 |
JJ |
denotes |
quantitative |
T22034 |
1111-1117 |
NN |
denotes |
system |
T22033 |
1092-1093 |
DT |
denotes |
a |
T22032 |
1086-1091 |
VBG |
denotes |
using |
T22031 |
1084-1085 |
-RRB- |
denotes |
) |
T22030 |
1074-1084 |
NNP |
denotes |
Stratagene |
T22029 |
1073-1074 |
-LRB- |
denotes |
( |
T22028 |
1058-1063 |
NNP |
denotes |
Green |
T22027 |
1053-1057 |
NNP |
denotes |
SYBR |
T22026 |
1040-1052 |
JJ |
denotes |
conventional |
T22025 |
1064-1072 |
NN |
denotes |
protocol |
T22024 |
1036-1039 |
DT |
denotes |
the |
T22023 |
1033-1035 |
IN |
denotes |
to |
T22022 |
1023-1032 |
VBG |
denotes |
according |
T22021 |
1009-1012 |
VBD |
denotes |
was |
T22020 |
1013-1022 |
VBN |
denotes |
performed |
T22019 |
992-1004 |
JJ |
denotes |
quantitative |
T22018 |
1005-1008 |
NN |
denotes |
PCR |
T22017 |
983-991 |
JJ |
denotes |
Relative |
T22016 |
982-1140 |
sentence |
denotes |
Relative quantitative PCR was performed according to the conventional SYBR Green protocol (Stratagene) using a quantitative PCR system (Mx3000p; Stratagene). |
T22015 |
981-982 |
. |
denotes |
. |
T22014 |
980-981 |
-RRB- |
denotes |
] |
T22013 |
978-980 |
CD |
denotes |
37 |
T22012 |
977-978 |
-LRB- |
denotes |
[ |
T22011 |
968-976 |
NN |
denotes |
software |
T22010 |
960-967 |
NN |
denotes |
Primer3 |
T22009 |
954-959 |
VBG |
denotes |
using |
T22008 |
952-953 |
-RRB- |
denotes |
) |
T22007 |
930-932 |
, |
denotes |
, |
T22006 |
906-930 |
NN |
denotes |
TGATTGAAGGGCTACTAGAGTGGT |
T22005 |
932-952 |
NN |
denotes |
AGCCCCAGAGGACAATTTTT |
T22004 |
905-906 |
-LRB- |
denotes |
( |
T22003 |
900-904 |
NN |
denotes |
Tia1 |
T22002 |
896-899 |
CC |
denotes |
and |
T22001 |
894-895 |
-RRB- |
denotes |
) |
T22000 |
872-874 |
, |
denotes |
, |
T21999 |
852-872 |
NN |
denotes |
TTGTGAGCTGTAGCCAGGTG |
T21998 |
874-894 |
NN |
denotes |
TGTGCACAGCTTGTCTCTCC |
T21997 |
851-852 |
-LRB- |
denotes |
( |
T21996 |
846-850 |
NN |
denotes |
Ren2 |
T21995 |
844-845 |
: |
denotes |
; |
T21994 |
843-844 |
-RRB- |
denotes |
) |
T21993 |
821-823 |
, |
denotes |
, |
T21992 |
801-821 |
NN |
denotes |
TTCTGGGAACTTGTGCACTG |
T21991 |
823-843 |
NN |
denotes |
AACTTCCTCCAGCCCATTTT |
T21990 |
800-801 |
-LRB- |
denotes |
( |
T21989 |
794-799 |
NN |
denotes |
Pygo2 |
T21988 |
792-793 |
: |
denotes |
; |
T21987 |
791-792 |
-RRB- |
denotes |
) |
T21986 |
770-772 |
, |
denotes |
, |
T21985 |
750-770 |
NN |
denotes |
GGGAGGGAACAGAAATCCTT |
T21984 |
772-791 |
NN |
denotes |
GCACCGATCAACAGTGCAG |
T21983 |
749-750 |
-LRB- |
denotes |
( |
T21982 |
742-748 |
NN |
denotes |
Picalm |
T21981 |
740-741 |
: |
denotes |
; |
T21980 |
739-740 |
-RRB- |
denotes |
) |
T21979 |
717-719 |
, |
denotes |
, |
T21978 |
697-717 |
NN |
denotes |
GCAGCATTACACCCGTCATA |
T21977 |
719-739 |
NN |
denotes |
TTGCCTCCATCCATATCTCC |
T21976 |
696-697 |
-LRB- |
denotes |
( |
T21975 |
691-695 |
NN |
denotes |
Klk5 |
T21974 |
689-690 |
: |
denotes |
; |
T21973 |
688-689 |
-RRB- |
denotes |
) |
T21972 |
666-668 |
, |
denotes |
, |
T21971 |
646-666 |
NN |
denotes |
CAGCATAGCCCAGGGTAGAG |
T21970 |
668-688 |
NN |
denotes |
TGGGCAAGGTAGTGAAGGTT |
T21969 |
645-646 |
-LRB- |
denotes |
( |
T21968 |
639-644 |
NN |
denotes |
Csrp1 |
T21967 |
637-638 |
: |
denotes |
; |
T21966 |
636-637 |
-RRB- |
denotes |
) |
T21965 |
614-616 |
, |
denotes |
, |
T21964 |
594-614 |
NN |
denotes |
GCAGACAGGCATCTTCACCT |
T21963 |
616-636 |
NN |
denotes |
TGTGTACATCATGGGCTCGT |
T21962 |
593-594 |
-LRB- |
denotes |
( |
T21961 |
586-592 |
NN |
denotes |
Col8a1 |
T21960 |
584-585 |
: |
denotes |
; |
T21959 |
583-584 |
-RRB- |
denotes |
) |
T21958 |
558-560 |
, |
denotes |
, |
T21957 |
538-558 |
NN |
denotes |
CCAGAAAGTGGTGTTTTGCT |
T21956 |
560-583 |
NN |
denotes |
GAGTTACAGAGAGCTTGCACCTT |
T21955 |
537-538 |
-LRB- |
denotes |
( |
T21954 |
529-536 |
NN |
denotes |
Aldh1a7 |
T21953 |
527-528 |
: |
denotes |
; |
T21952 |
526-527 |
-RRB- |
denotes |
) |
T21951 |
504-506 |
, |
denotes |
, |
T21950 |
484-504 |
NN |
denotes |
TTGCTGACAGGATGCAGAAG |
T21949 |
506-526 |
NN |
denotes |
ACATCTGCTGGAAGGTGGAC |
T21948 |
483-484 |
-LRB- |
denotes |
( |
T21947 |
478-482 |
NN |
denotes |
Actb |
T21946 |
476-478 |
: |
denotes |
: |
T21945 |
462-470 |
NNP |
denotes |
Illumina |
T21944 |
471-476 |
NN |
denotes |
probe |
T21943 |
458-461 |
DT |
denotes |
the |
T21942 |
453-457 |
IN |
denotes |
from |
T21941 |
444-452 |
VBN |
denotes |
obtained |
T21940 |
435-443 |
NN |
denotes |
sequence |
T21939 |
431-434 |
DT |
denotes |
the |
T21938 |
423-430 |
VB |
denotes |
include |
T21937 |
420-422 |
TO |
denotes |
to |
T21936 |
405-409 |
VBD |
denotes |
were |
T21935 |
410-419 |
VBN |
denotes |
generated |
T21934 |
387-396 |
VBG |
denotes |
following |
T21933 |
397-404 |
NNS |
denotes |
primers |
T21932 |
383-386 |
DT |
denotes |
The |
T21931 |
382-982 |
sentence |
denotes |
The following primers were generated to include the sequence obtained from the Illumina probe: Actb (TTGCTGACAGGATGCAGAAG, ACATCTGCTGGAAGGTGGAC); Aldh1a7 (CCAGAAAGTGGTGTTTTGCT, GAGTTACAGAGAGCTTGCACCTT); Col8a1 (GCAGACAGGCATCTTCACCT, TGTGTACATCATGGGCTCGT); Csrp1 (CAGCATAGCCCAGGGTAGAG, TGGGCAAGGTAGTGAAGGTT); Klk5 (GCAGCATTACACCCGTCATA, TTGCCTCCATCCATATCTCC); Picalm (GGGAGGGAACAGAAATCCTT, GCACCGATCAACAGTGCAG); Pygo2 (TTCTGGGAACTTGTGCACTG, AACTTCCTCCAGCCCATTTT); Ren2 (TTGTGAGCTGTAGCCAGGTG, TGTGCACAGCTTGTCTCTCC) and Tia1 (TGATTGAAGGGCTACTAGAGTGGT, AGCCCCAGAGGACAATTTTT) using Primer3 software [37]. |
T21930 |
381-382 |
. |
denotes |
. |
T21929 |
380-381 |
-RRB- |
denotes |
) |
T21928 |
377-380 |
NNP |
denotes |
USA |
T21927 |
375-377 |
, |
denotes |
, |
T21926 |
373-375 |
NNP |
denotes |
CA |
T21925 |
371-373 |
, |
denotes |
, |
T21865 |
50-57 |
NNS |
denotes |
kidneys |
T21864 |
15-20 |
NN |
denotes |
E18.5 |
T21863 |
10-14 |
IN |
denotes |
from |
T21862 |
152-160 |
VBN |
denotes |
purified |
T21861 |
6-9 |
NN |
denotes |
RNA |
T21860 |
0-5 |
JJ |
denotes |
Total |
T22081 |
1365-1371 |
NN |
denotes |
method |
T22080 |
1340-1343 |
DT |
denotes |
the |
T22095 |
1376-1377 |
. |
denotes |
. |
T22094 |
1375-1376 |
-RRB- |
denotes |
] |
T22093 |
1373-1375 |
CD |
denotes |
38 |
T22092 |
1372-1373 |
-LRB- |
denotes |
[ |
T22091 |
1363-1364 |
-RRB- |
denotes |
) |
T22090 |
1362-1363 |
-RRB- |
denotes |
) |
T22089 |
1360-1361 |
-LRB- |
denotes |
( |
T22088 |
1359-1360 |
NN |
denotes |
C |
T22087 |
1353-1358 |
NN |
denotes |
Delta |
T22086 |
1347-1352 |
NN |
denotes |
Delta |
T22085 |
1346-1347 |
HYPH |
denotes |
- |
T22084 |
1345-1346 |
-LRB- |
denotes |
( |
T22083 |
1361-1362 |
NN |
denotes |
T |
T22082 |
1344-1345 |
CD |
denotes |
2 |
T22079 |
1334-1339 |
VBG |
denotes |
using |
T22078 |
1325-1333 |
VBN |
denotes |
analyzed |
T22077 |
1321-1324 |
CC |
denotes |
and |
T22076 |
1319-1320 |
-RRB- |
denotes |
) |
R6175 |
T21860 |
T21861 |
amod |
Total,RNA |
R6176 |
T21861 |
T21862 |
nsubjpass |
RNA,purified |
R6177 |
T21863 |
T21861 |
prep |
from,RNA |
R6178 |
T21864 |
T21865 |
nmod |
E18.5,kidneys |
R6179 |
T21865 |
T21863 |
pobj |
kidneys,from |
R6180 |
T21866 |
T21867 |
compound |
Pygo1,Pygo2 |
R6181 |
T21867 |
T21869 |
npadvmod |
Pygo2,null |
R6182 |
T21868 |
T21867 |
punct |
/,Pygo2 |
R6183 |
T21869 |
T21865 |
amod |
null,kidneys |
R6184 |
T21870 |
T21869 |
cc |
and,null |
R6185 |
T21871 |
T21869 |
conj |
control,null |
R6186 |
T21872 |
T21861 |
punct |
(,RNA |
R6187 |
T21873 |
T21874 |
preconj |
both,represented |
R6188 |
T21874 |
T21861 |
acl |
represented,RNA |
R6189 |
T21875 |
T21874 |
prep |
in,represented |
R6190 |
T21876 |
T21875 |
pobj |
duplicate,in |
R6191 |
T21877 |
T21874 |
cc |
and,represented |
R6192 |
T21878 |
T21874 |
conj |
distinct,represented |
R6193 |
T21879 |
T21878 |
prep |
from,distinct |
R6194 |
T21880 |
T21881 |
det |
the,kidneys |
R6195 |
T21881 |
T21879 |
pobj |
kidneys,from |
R6196 |
T21882 |
T21881 |
acl |
used,kidneys |
R6197 |
T21883 |
T21882 |
prep |
for,used |
R6198 |
T21884 |
T21885 |
compound |
microrray,analysis |
R6199 |
T21885 |
T21883 |
pobj |
analysis,for |
R6200 |
T21886 |
T21862 |
punct |
),purified |
R6201 |
T21887 |
T21862 |
auxpass |
was,purified |
R6202 |
T21888 |
T21862 |
advcl |
using,purified |
R6203 |
T21889 |
T21890 |
det |
a,kit |
R6204 |
T21890 |
T21888 |
dobj |
kit,using |
R6205 |
T21891 |
T21890 |
amod |
commercial,kit |
R6206 |
T21892 |
T21890 |
punct |
(,kit |
R6207 |
T21893 |
T21894 |
compound |
Absolutely,Kit |
R6208 |
T21894 |
T21890 |
appos |
Kit,kit |
R6209 |
T21895 |
T21894 |
compound |
RNA,Kit |
R6210 |
T21896 |
T21894 |
compound |
Microprep,Kit |
R6211 |
T21897 |
T21898 |
punct |
;,Stratagene |
R6212 |
T21898 |
T21890 |
parataxis |
Stratagene,kit |
R6213 |
T21899 |
T21898 |
punct |
", ",Stratagene |
R6214 |
T21900 |
T21901 |
compound |
La,Jolla |
R6215 |
T21901 |
T21898 |
npadvmod |
Jolla,Stratagene |
R6216 |
T21902 |
T21898 |
punct |
", ",Stratagene |
R6217 |
T21903 |
T21904 |
compound |
CA,USA |
R6218 |
T21904 |
T21898 |
npadvmod |
USA,Stratagene |
R6219 |
T21905 |
T21898 |
punct |
),Stratagene |
R6220 |
T21906 |
T21890 |
prep |
including,kit |
R6221 |
T21907 |
T21908 |
compound |
DNAse1,treatment |
R6222 |
T21908 |
T21906 |
pobj |
treatment,including |
R6223 |
T21909 |
T21862 |
punct |
.,purified |
R6224 |
T21911 |
T21912 |
nsubjpass |
cDNA,generated |
R6225 |
T21913 |
T21912 |
auxpass |
was,generated |
R6226 |
T21914 |
T21912 |
advcl |
using,generated |
R6227 |
T21915 |
T21916 |
amod |
random,hexamers |
R6228 |
T21916 |
T21914 |
dobj |
hexamers,using |
R6229 |
T21917 |
T21914 |
prep |
according,using |
R6230 |
T21918 |
T21917 |
prep |
to,according |
R6231 |
T21919 |
T21920 |
amod |
conventional,protocols |
R6232 |
T21920 |
T21918 |
pobj |
protocols,to |
R6233 |
T21921 |
T21922 |
punct |
(,Invitrogen |
R6234 |
T21922 |
T21920 |
parataxis |
Invitrogen,protocols |
R6235 |
T21923 |
T21922 |
punct |
", ",Invitrogen |
R6236 |
T21924 |
T21922 |
npadvmod |
Carlsbad,Invitrogen |
R6237 |
T21925 |
T21922 |
punct |
", ",Invitrogen |
R6238 |
T21926 |
T21922 |
npadvmod |
CA,Invitrogen |
R6239 |
T21927 |
T21922 |
punct |
", ",Invitrogen |
R6240 |
T21928 |
T21922 |
npadvmod |
USA,Invitrogen |
R6241 |
T21929 |
T21922 |
punct |
),Invitrogen |
R6242 |
T21930 |
T21912 |
punct |
.,generated |
R6243 |
T21932 |
T21933 |
det |
The,primers |
R6244 |
T21933 |
T21935 |
nsubjpass |
primers,generated |
R6245 |
T21934 |
T21933 |
amod |
following,primers |
R6246 |
T21936 |
T21935 |
auxpass |
were,generated |
R6247 |
T21937 |
T21938 |
aux |
to,include |
R6248 |
T21938 |
T21935 |
advcl |
include,generated |
R6249 |
T21939 |
T21940 |
det |
the,sequence |
R6250 |
T21940 |
T21938 |
dobj |
sequence,include |
R6251 |
T21941 |
T21940 |
acl |
obtained,sequence |
R6252 |
T21942 |
T21941 |
prep |
from,obtained |
R6253 |
T21943 |
T21944 |
det |
the,probe |
R6254 |
T21944 |
T21942 |
pobj |
probe,from |
R6255 |
T21945 |
T21944 |
compound |
Illumina,probe |
R6256 |
T21946 |
T21935 |
punct |
: ,generated |
R6257 |
T21947 |
T21935 |
dobj |
Actb,generated |
R6258 |
T21948 |
T21949 |
punct |
(,ACATCTGCTGGAAGGTGGAC |
R6259 |
T21949 |
T21947 |
parataxis |
ACATCTGCTGGAAGGTGGAC,Actb |
R6260 |
T21950 |
T21949 |
nmod |
TTGCTGACAGGATGCAGAAG,ACATCTGCTGGAAGGTGGAC |
R6261 |
T21951 |
T21949 |
punct |
", ",ACATCTGCTGGAAGGTGGAC |
R6262 |
T21952 |
T21949 |
punct |
),ACATCTGCTGGAAGGTGGAC |
R6263 |
T21953 |
T21947 |
punct |
;,Actb |
R6264 |
T21954 |
T21947 |
conj |
Aldh1a7,Actb |
R6265 |
T21955 |
T21956 |
punct |
(,GAGTTACAGAGAGCTTGCACCTT |
R6266 |
T21956 |
T21954 |
parataxis |
GAGTTACAGAGAGCTTGCACCTT,Aldh1a7 |
R6267 |
T21957 |
T21956 |
nmod |
CCAGAAAGTGGTGTTTTGCT,GAGTTACAGAGAGCTTGCACCTT |
R6268 |
T21958 |
T21956 |
punct |
", ",GAGTTACAGAGAGCTTGCACCTT |
R6269 |
T21959 |
T21956 |
punct |
),GAGTTACAGAGAGCTTGCACCTT |
R6270 |
T21960 |
T21954 |
punct |
;,Aldh1a7 |
R6271 |
T21961 |
T21954 |
conj |
Col8a1,Aldh1a7 |
R6272 |
T21962 |
T21963 |
punct |
(,TGTGTACATCATGGGCTCGT |
R6273 |
T21963 |
T21961 |
parataxis |
TGTGTACATCATGGGCTCGT,Col8a1 |
R6274 |
T21964 |
T21963 |
nmod |
GCAGACAGGCATCTTCACCT,TGTGTACATCATGGGCTCGT |
R6275 |
T21965 |
T21963 |
punct |
", ",TGTGTACATCATGGGCTCGT |
R6276 |
T21966 |
T21963 |
punct |
),TGTGTACATCATGGGCTCGT |
R6277 |
T21967 |
T21961 |
punct |
;,Col8a1 |
R6278 |
T21968 |
T21961 |
conj |
Csrp1,Col8a1 |
R6279 |
T21969 |
T21970 |
punct |
(,TGGGCAAGGTAGTGAAGGTT |
R6280 |
T21970 |
T21968 |
parataxis |
TGGGCAAGGTAGTGAAGGTT,Csrp1 |
R6281 |
T21971 |
T21970 |
nmod |
CAGCATAGCCCAGGGTAGAG,TGGGCAAGGTAGTGAAGGTT |
R6282 |
T21972 |
T21970 |
punct |
", ",TGGGCAAGGTAGTGAAGGTT |
R6283 |
T21973 |
T21970 |
punct |
),TGGGCAAGGTAGTGAAGGTT |
R6284 |
T21974 |
T21968 |
punct |
;,Csrp1 |
R6285 |
T21975 |
T21968 |
conj |
Klk5,Csrp1 |
R6286 |
T21976 |
T21977 |
punct |
(,TTGCCTCCATCCATATCTCC |
R6287 |
T21977 |
T21975 |
parataxis |
TTGCCTCCATCCATATCTCC,Klk5 |
R6288 |
T21978 |
T21977 |
nmod |
GCAGCATTACACCCGTCATA,TTGCCTCCATCCATATCTCC |
R6289 |
T21979 |
T21977 |
punct |
", ",TTGCCTCCATCCATATCTCC |
R6290 |
T21980 |
T21977 |
punct |
),TTGCCTCCATCCATATCTCC |
R6291 |
T21981 |
T21975 |
punct |
;,Klk5 |
R6292 |
T21982 |
T21975 |
conj |
Picalm,Klk5 |
R6293 |
T21983 |
T21984 |
punct |
(,GCACCGATCAACAGTGCAG |
R6294 |
T21984 |
T21982 |
parataxis |
GCACCGATCAACAGTGCAG,Picalm |
R6295 |
T21985 |
T21984 |
nmod |
GGGAGGGAACAGAAATCCTT,GCACCGATCAACAGTGCAG |
R6296 |
T21986 |
T21984 |
punct |
", ",GCACCGATCAACAGTGCAG |
R6297 |
T21987 |
T21984 |
punct |
),GCACCGATCAACAGTGCAG |
R6298 |
T21988 |
T21982 |
punct |
;,Picalm |
R6299 |
T21989 |
T21982 |
conj |
Pygo2,Picalm |
R6300 |
T21990 |
T21991 |
punct |
(,AACTTCCTCCAGCCCATTTT |
R6301 |
T21991 |
T21989 |
parataxis |
AACTTCCTCCAGCCCATTTT,Pygo2 |
R6302 |
T21992 |
T21991 |
nmod |
TTCTGGGAACTTGTGCACTG,AACTTCCTCCAGCCCATTTT |
R6303 |
T21993 |
T21991 |
punct |
", ",AACTTCCTCCAGCCCATTTT |
R6304 |
T21994 |
T21991 |
punct |
),AACTTCCTCCAGCCCATTTT |
R6305 |
T21995 |
T21989 |
punct |
;,Pygo2 |
R6306 |
T21996 |
T21989 |
conj |
Ren2,Pygo2 |
R6307 |
T21997 |
T21998 |
punct |
(,TGTGCACAGCTTGTCTCTCC |
R6308 |
T21998 |
T21996 |
parataxis |
TGTGCACAGCTTGTCTCTCC,Ren2 |
R6309 |
T21999 |
T21998 |
nmod |
TTGTGAGCTGTAGCCAGGTG,TGTGCACAGCTTGTCTCTCC |
R6310 |
T22000 |
T21998 |
punct |
", ",TGTGCACAGCTTGTCTCTCC |
R6311 |
T22001 |
T21998 |
punct |
),TGTGCACAGCTTGTCTCTCC |
R6312 |
T22002 |
T21996 |
cc |
and,Ren2 |
R6313 |
T22003 |
T21996 |
conj |
Tia1,Ren2 |
R6314 |
T22004 |
T22005 |
punct |
(,AGCCCCAGAGGACAATTTTT |
R6315 |
T22005 |
T22003 |
parataxis |
AGCCCCAGAGGACAATTTTT,Tia1 |
R6316 |
T22006 |
T22005 |
nmod |
TGATTGAAGGGCTACTAGAGTGGT,AGCCCCAGAGGACAATTTTT |
R6317 |
T22007 |
T22005 |
punct |
", ",AGCCCCAGAGGACAATTTTT |
R6318 |
T22008 |
T22005 |
punct |
),AGCCCCAGAGGACAATTTTT |
R6319 |
T22009 |
T21935 |
advcl |
using,generated |
R6320 |
T22010 |
T22011 |
compound |
Primer3,software |
R6321 |
T22011 |
T22009 |
dobj |
software,using |
R6322 |
T22012 |
T22013 |
punct |
[,37 |
R6323 |
T22013 |
T22011 |
parataxis |
37,software |
R6324 |
T22014 |
T22013 |
punct |
],37 |
R6325 |
T22015 |
T21935 |
punct |
.,generated |
R6326 |
T22017 |
T22018 |
amod |
Relative,PCR |
R6327 |
T22018 |
T22020 |
nsubjpass |
PCR,performed |
R6328 |
T22019 |
T22018 |
amod |
quantitative,PCR |
R6329 |
T22021 |
T22020 |
auxpass |
was,performed |
R6330 |
T22022 |
T22020 |
prep |
according,performed |
R6331 |
T22023 |
T22022 |
prep |
to,according |
R6332 |
T22024 |
T22025 |
det |
the,protocol |
R6333 |
T22025 |
T22023 |
pobj |
protocol,to |
R6334 |
T22026 |
T22025 |
amod |
conventional,protocol |
R6335 |
T22027 |
T22028 |
compound |
SYBR,Green |
R6336 |
T22028 |
T22025 |
compound |
Green,protocol |
R6337 |
T22029 |
T22030 |
punct |
(,Stratagene |
R6338 |
T22030 |
T22025 |
parataxis |
Stratagene,protocol |
R6339 |
T22031 |
T22030 |
punct |
),Stratagene |
R6340 |
T22032 |
T22020 |
advcl |
using,performed |
R6341 |
T22033 |
T22034 |
det |
a,system |
R6342 |
T22034 |
T22032 |
dobj |
system,using |
R6343 |
T22035 |
T22034 |
amod |
quantitative,system |
R6344 |
T22036 |
T22034 |
compound |
PCR,system |
R6345 |
T22037 |
T22038 |
punct |
(,Stratagene |
R6346 |
T22038 |
T22032 |
parataxis |
Stratagene,using |
R6347 |
T22039 |
T22038 |
dep |
Mx3000p,Stratagene |
R6348 |
T22040 |
T22038 |
punct |
;,Stratagene |
R6349 |
T22041 |
T22038 |
punct |
),Stratagene |
R6350 |
T22042 |
T22020 |
punct |
.,performed |
R6351 |
T22044 |
T22045 |
nmod |
Dissociation,curve |
R6352 |
T22045 |
T22046 |
nmod |
curve,analysis |
R6353 |
T22046 |
T22051 |
nsubjpass |
analysis,used |
R6354 |
T22047 |
T22045 |
cc |
and,curve |
R6355 |
T22048 |
T22049 |
compound |
agarose,gel |
R6356 |
T22049 |
T22045 |
conj |
gel,curve |
R6357 |
T22050 |
T22049 |
punct |
-,gel |
R6358 |
T22052 |
T22046 |
prep |
of,analysis |
R6359 |
T22053 |
T22054 |
det |
each,primer |
R6360 |
T22054 |
T22052 |
pobj |
primer,of |
R6361 |
T22055 |
T22054 |
amod |
set,primer |
R6362 |
T22056 |
T22051 |
auxpass |
were,used |
R6363 |
T22057 |
T22058 |
aux |
to,insure |
R6364 |
T22058 |
T22051 |
advcl |
insure,used |
R6365 |
T22059 |
T22058 |
dobj |
specificity,insure |
R6366 |
T22060 |
T22059 |
prep |
of,specificity |
R6367 |
T22061 |
T22062 |
det |
the,amplicon |
R6368 |
T22062 |
T22060 |
pobj |
amplicon,of |
R6369 |
T22063 |
T22051 |
punct |
.,used |
R6370 |
T22065 |
T22066 |
det |
All,data |
R6371 |
T22066 |
T22067 |
nsubjpass |
data,normalized |
R6372 |
T22068 |
T22067 |
auxpass |
were,normalized |
R6373 |
T22069 |
T22067 |
prep |
to,normalized |
R6374 |
T22070 |
T22071 |
det |
an,control |
R6375 |
T22071 |
T22069 |
pobj |
control,to |
R6376 |
T22072 |
T22071 |
amod |
internal,control |
R6377 |
T22073 |
T22071 |
compound |
housekeeping,control |
R6378 |
T22074 |
T22071 |
punct |
(,control |
R6379 |
T22075 |
T22071 |
appos |
Actb,control |
R6380 |
T22076 |
T22067 |
punct |
),normalized |
R6381 |
T22077 |
T22067 |
cc |
and,normalized |
R6382 |
T22078 |
T22067 |
conj |
analyzed,normalized |
R6383 |
T22079 |
T22078 |
advcl |
using,analyzed |
R6384 |
T22080 |
T22081 |
det |
the,method |
R6385 |
T22081 |
T22079 |
dobj |
method,using |
R6386 |
T22082 |
T22083 |
nummod |
2,T |
R6387 |
T22083 |
T22081 |
nmod |
T,method |
R6388 |
T22084 |
T22083 |
punct |
(,T |
R6389 |
T22085 |
T22083 |
punct |
-,T |
R6390 |
T22086 |
T22083 |
nmod |
Delta,T |
R6391 |
T22087 |
T22083 |
nmod |
Delta,T |
R6392 |
T22088 |
T22083 |
nmod |
C,T |
R6393 |
T22089 |
T22083 |
punct |
(,T |
R6394 |
T22090 |
T22083 |
punct |
),T |
R6395 |
T22091 |
T22083 |
punct |
),T |
R6396 |
T22092 |
T22093 |
punct |
[,38 |
R6397 |
T22093 |
T22078 |
parataxis |
38,analyzed |
R6398 |
T22094 |
T22093 |
punct |
],38 |
R6399 |
T22095 |
T22067 |
punct |
.,normalized |