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transgene reporter assay of canonical Wnt signaling\nX-gal staining of both embryos and developing kidneys was performed as previously described [21]. Care was taken to reduce endogenous β-galactosidase activity within the developing kidney by increasing pH of the X-gal staining solution to 8.0. Changes in transgene β-Gal expression were quantitated using a β-Gal ELISA (Enzyme-linked immunoassay) kit (Roche, Indianapolis, IN), normalizing according to total protein. Each genotype was represented by a sample size of 4 except the non-transgenic (n = 5), Pygo1+/- ; Pygo2+/+ (n = 6), Pygo1-/- ; Pygo2+/- (n = 8), and Pygo1-/- ; Pygo2-/- (n = 6) groups."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T20632","span":{"begin":645,"end":646},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20631","span":{"begin":643,"end":644},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20630","span":{"begin":638,"end":643},"obj":"PR_EXT:000013524"},{"id":"T20629","span":{"begin":634,"end":635},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20628","span":{"begin":632,"end":633},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20627","span":{"begin":627,"end":632},"obj":"PR_EXT:000013523"},{"id":"T20626","span":{"begin":612,"end":613},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20625","span":{"begin":610,"end":611},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T20624","span":{"begin":605,"end":610},"obj":"PR_EXT:000013524"},{"id":"T20623","span":{"begin":601,"end":602},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20622","span":{"begin":599,"end":600},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20621","span":{"begin":594,"end":599},"obj":"PR_EXT:000013523"},{"id":"T20620","span":{"begin":583,"end":584},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T20619","span":{"begin":581,"end":582},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T20618","span":{"begin":576,"end":581},"obj":"PR_EXT:000013524"},{"id":"T20617","span":{"begin":572,"end":573},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T20616","span":{"begin":570,"end":571},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T20615","span":{"begin":565,"end":570},"obj":"PR_EXT:000013523"},{"id":"T20614","span":{"begin":545,"end":555},"obj":"SO_EXT:transgenic_entity"},{"id":"T20613","span":{"begin":483,"end":491},"obj":"SO_EXT:genotype_or_entity_with_genotype"},{"id":"T20612","span":{"begin":469,"end":476},"obj":"CHEBI_PR_EXT:protein"},{"id":"T20611","span":{"begin":380,"end":386},"obj":"CHEBI_GO_SO_EXT:enzyme"},{"id":"T20610","span":{"begin":367,"end":372},"obj":"GO_EXT:0004565"},{"id":"T20609","span":{"begin":331,"end":341},"obj":"GO:0010467"},{"id":"T20608","span":{"begin":325,"end":330},"obj":"GO_EXT:0004565"},{"id":"T20607","span":{"begin":315,"end":324},"obj":"SO_EXT:0000902"},{"id":"T20606","span":{"begin":287,"end":295},"obj":"CHEBI:75958"},{"id":"T20605","span":{"begin":272,"end":277},"obj":"CHEBI:75055"},{"id":"T20604","span":{"begin":241,"end":247},"obj":"UBERON:0002113"},{"id":"T20603","span":{"begin":194,"end":209},"obj":"GO_EXT:0004565"},{"id":"T20602","span":{"begin":106,"end":113},"obj":"UBERON:0002113"},{"id":"T20601","span":{"begin":83,"end":90},"obj":"UBERON:0000922"},{"id":"T20600","span":{"begin":60,"end":65},"obj":"CHEBI:75055"},{"id":"T20599","span":{"begin":36,"end":59},"obj":"GO:0060070"},{"id":"T20598","span":{"begin":8,"end":17},"obj":"SO_EXT:0000902"},{"id":"T20597","span":{"begin":4,"end":7},"obj":"GO_EXT:0015925"}],"text":"BAT-gal transgene reporter assay of canonical Wnt signaling\nX-gal staining of both embryos and developing kidneys was performed as previously described [21]. Care was taken to reduce endogenous β-galactosidase activity within the developing kidney by increasing pH of the X-gal staining solution to 8.0. Changes in transgene β-Gal expression were quantitated using a β-Gal ELISA (Enzyme-linked immunoassay) kit (Roche, Indianapolis, IN), normalizing according to total protein. Each genotype was represented by a sample size of 4 except the non-transgenic (n = 5), Pygo1+/- ; Pygo2+/+ (n = 6), Pygo1-/- ; Pygo2+/- (n = 8), and Pygo1-/- ; Pygo2-/- (n = 6) groups."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T20592","span":{"begin":576,"end":581},"obj":"PR:000013524"},{"id":"T20591","span":{"begin":565,"end":570},"obj":"PR:000013523"},{"id":"T20589","span":{"begin":315,"end":324},"obj":"SO:0000902"},{"id":"T20588","span":{"begin":287,"end":295},"obj":"CHEBI:75958"},{"id":"T20587","span":{"begin":272,"end":277},"obj":"CHEBI:75055"},{"id":"T20586","span":{"begin":241,"end":247},"obj":"UBERON:0002113"},{"id":"T20585","span":{"begin":106,"end":113},"obj":"UBERON:0002113"},{"id":"T20584","span":{"begin":83,"end":90},"obj":"UBERON:0000922"},{"id":"T20583","span":{"begin":60,"end":65},"obj":"CHEBI:75055"},{"id":"T20582","span":{"begin":36,"end":59},"obj":"GO:0060070"},{"id":"T20581","span":{"begin":8,"end":17},"obj":"SO:0000902"},{"id":"T20596","span":{"begin":638,"end":643},"obj":"PR:000013524"},{"id":"T20595","span":{"begin":627,"end":632},"obj":"PR:000013523"},{"id":"T20594","span":{"begin":605,"end":610},"obj":"PR:000013524"},{"id":"T20593","span":{"begin":594,"end":599},"obj":"PR:000013523"},{"id":"T20590","span":{"begin":331,"end":341},"obj":"GO:0010467"}],"text":"BAT-gal transgene reporter assay of canonical Wnt signaling\nX-gal staining of both embryos and developing kidneys was performed as previously described [21]. Care was taken to reduce endogenous β-galactosidase activity within the developing kidney by increasing pH of the X-gal staining solution to 8.0. Changes in transgene β-Gal expression were quantitated using a β-Gal ELISA (Enzyme-linked immunoassay) kit (Roche, Indianapolis, IN), normalizing according to total protein. Each genotype was represented by a sample size of 4 except the non-transgenic (n = 5), Pygo1+/- ; Pygo2+/+ (n = 6), Pygo1-/- ; Pygo2+/- (n = 8), and Pygo1-/- ; Pygo2-/- (n = 6) groups."}

{"project":"2_test","denotations":[{"id":"17425782-12626757-8469490","span":{"begin":153,"end":155},"obj":"12626757"}],"text":"BAT-gal transgene reporter assay of canonical Wnt signaling\nX-gal staining of both embryos and developing kidneys was performed as previously described [21]. Care was taken to reduce endogenous β-galactosidase activity within the developing kidney by increasing pH of the X-gal staining solution to 8.0. Changes in transgene β-Gal expression were quantitated using a β-Gal ELISA (Enzyme-linked immunoassay) kit (Roche, Indianapolis, IN), normalizing according to total protein. Each genotype was represented by a sample size of 4 except the non-transgenic (n = 5), Pygo1+/- ; Pygo2+/+ (n = 6), Pygo1-/- ; Pygo2+/- (n = 8), and Pygo1-/- ; Pygo2-/- (n = 6) groups."}