PMC:1858683 / 40109-41426 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T19252","span":{"begin":683,"end":694},"obj":"JJ"},{"id":"T19251","span":{"begin":671,"end":672},"obj":"HYPH"},{"id":"T19250","span":{"begin":668,"end":671},"obj":"CD"},{"id":"T19249","span":{"begin":672,"end":682},"obj":"VBN"},{"id":"T19248","span":{"begin":662,"end":667},"obj":"NNP"},{"id":"T19247","span":{"begin":657,"end":661},"obj":"VBD"},{"id":"T19246","span":{"begin":636,"end":645},"obj":"JJ"},{"id":"T19245","span":{"begin":646,"end":656},"obj":"NNS"},{"id":"T19244","span":{"begin":632,"end":635},"obj":"DT"},{"id":"T19243","span":{"begin":631,"end":776},"obj":"sentence"},{"id":"T19242","span":{"begin":630,"end":631},"obj":"."},{"id":"T19241","span":{"begin":629,"end":630},"obj":"-RRB-"},{"id":"T19240","span":{"begin":626,"end":629},"obj":"NNP"},{"id":"T19239","span":{"begin":624,"end":626},"obj":","},{"id":"T19238","span":{"begin":622,"end":624},"obj":"NNP"},{"id":"T19237","span":{"begin":620,"end":622},"obj":","},{"id":"T19236","span":{"begin":613,"end":620},"obj":"NNP"},{"id":"T19235","span":{"begin":611,"end":613},"obj":","},{"id":"T19234","span":{"begin":593,"end":599},"obj":"NNP"},{"id":"T19233","span":{"begin":589,"end":592},"obj":"NNP"},{"id":"T19232","span":{"begin":588,"end":589},"obj":"HYPH"},{"id":"T19231","span":{"begin":578,"end":588},"obj":"NNP"},{"id":"T19230","span":{"begin":600,"end":611},"obj":"NNP"},{"id":"T19229","span":{"begin":577,"end":578},"obj":"-LRB-"},{"id":"T19228","span":{"begin":565,"end":576},"obj":"JJ"},{"id":"T19227","span":{"begin":561,"end":564},"obj":"CC"},{"id":"T19226","span":{"begin":559,"end":561},"obj":","},{"id":"T19225","span":{"begin":558,"end":559},"obj":"-RRB-"},{"id":"T19224","span":{"begin":555,"end":558},"obj":"NNP"},{"id":"T19223","span":{"begin":553,"end":555},"obj":","},{"id":"T19222","span":{"begin":551,"end":553},"obj":"NNP"},{"id":"T19221","span":{"begin":549,"end":551},"obj":","},{"id":"T19220","span":{"begin":544,"end":549},"obj":"NNP"},{"id":"T19219","span":{"begin":540,"end":543},"obj":"NNP"},{"id":"T19218","span":{"begin":531,"end":532},"obj":"HYPH"},{"id":"T19217","span":{"begin":526,"end":531},"obj":"NNP"},{"id":"T19216","span":{"begin":532,"end":539},"obj":"NNP"},{"id":"T19215","span":{"begin":524,"end":526},"obj":","},{"id":"T19214","span":{"begin":520,"end":524},"obj":"NN"},{"id":"T19213","span":{"begin":518,"end":520},"obj":","},{"id":"T19212","span":{"begin":509,"end":510},"obj":"HYPH"},{"id":"T19211","span":{"begin":510,"end":518},"obj":"NN"},{"id":"T19210","span":{"begin":508,"end":509},"obj":"NN"},{"id":"T19209","span":{"begin":507,"end":508},"obj":"-LRB-"},{"id":"T19208","span":{"begin":491,"end":506},"obj":"JJ"},{"id":"T19207","span":{"begin":489,"end":491},"obj":","},{"id":"T19206","span":{"begin":488,"end":489},"obj":"-RRB-"},{"id":"T19205","span":{"begin":485,"end":488},"obj":"NNP"},{"id":"T19204","span":{"begin":483,"end":485},"obj":","},{"id":"T19203","span":{"begin":481,"end":483},"obj":"NNP"},{"id":"T19202","span":{"begin":479,"end":481},"obj":","},{"id":"T19201","span":{"begin":475,"end":479},"obj":"NNP"},{"id":"T19200","span":{"begin":469,"end":474},"obj":"NNP"},{"id":"T19199","span":{"begin":467,"end":469},"obj":","},{"id":"T19198","span":{"begin":449,"end":453},"obj":"NNP"},{"id":"T19197","span":{"begin":443,"end":448},"obj":"NNP"},{"id":"T19196","span":{"begin":441,"end":442},"obj":":"},{"id":"T19195","span":{"begin":439,"end":441},"obj":"CD"},{"id":"T19194","span":{"begin":438,"end":439},"obj":"HYPH"},{"id":"T19193","span":{"begin":437,"end":438},"obj":"NN"},{"id":"T19192","span":{"begin":454,"end":467},"obj":"NNP"},{"id":"T19191","span":{"begin":436,"end":437},"obj":"-LRB-"},{"id":"T19190","span":{"begin":427,"end":435},"obj":"JJ"},{"id":"T19189","span":{"begin":422,"end":426},"obj":"VBD"},{"id":"T19188","span":{"begin":403,"end":410},"obj":"JJ"},{"id":"T19187","span":{"begin":411,"end":421},"obj":"NNS"},{"id":"T19186","span":{"begin":399,"end":402},"obj":"DT"},{"id":"T19185","span":{"begin":398,"end":631},"obj":"sentence"},{"id":"T19184","span":{"begin":397,"end":398},"obj":"."},{"id":"T19183","span":{"begin":387,"end":397},"obj":"NNS"},{"id":"T19182","span":{"begin":383,"end":386},"obj":"DT"},{"id":"T19181","span":{"begin":378,"end":382},"obj":"IN"},{"id":"T19180","span":{"begin":366,"end":377},"obj":"NNS"},{"id":"T19179","span":{"begin":362,"end":365},"obj":"IN"},{"id":"T19178","span":{"begin":353,"end":356},"obj":"VBD"},{"id":"T19177","span":{"begin":342,"end":346},"obj":"NN"},{"id":"T19176","span":{"begin":347,"end":352},"obj":"NN"},{"id":"T19175","span":{"begin":340,"end":341},"obj":"NN"},{"id":"T19174","span":{"begin":339,"end":340},"obj":"CD"},{"id":"T19173","span":{"begin":334,"end":338},"obj":"CC"},{"id":"T19172","span":{"begin":357,"end":361},"obj":"VBN"},{"id":"T19171","span":{"begin":331,"end":332},"obj":"HYPH"},{"id":"T19170","span":{"begin":332,"end":333},"obj":"NN"},{"id":"T19169","span":{"begin":328,"end":331},"obj":"NN"},{"id":"T19168","span":{"begin":324,"end":327},"obj":"CC"},{"id":"T19167","span":{"begin":322,"end":324},"obj":","},{"id":"T19166","span":{"begin":321,"end":322},"obj":"-RRB-"},{"id":"T19165","span":{"begin":318,"end":321},"obj":"NNP"},{"id":"T19164","span":{"begin":316,"end":318},"obj":","},{"id":"T19163","span":{"begin":306,"end":316},"obj":"NNP"},{"id":"T19162","span":{"begin":304,"end":305},"obj":":"},{"id":"T19161","span":{"begin":298,"end":304},"obj":"NNP"},{"id":"T19160","span":{"begin":296,"end":298},"obj":","},{"id":"T19159","span":{"begin":293,"end":296},"obj":"NNP"},{"id":"T19158","span":{"begin":292,"end":293},"obj":"-LRB-"},{"id":"T19157","span":{"begin":273,"end":281},"obj":"NN"},{"id":"T19156","span":{"begin":264,"end":272},"obj":"NNP"},{"id":"T19155","span":{"begin":282,"end":291},"obj":"NN"},{"id":"T19154","span":{"begin":252,"end":253},"obj":"HYPH"},{"id":"T19153","span":{"begin":253,"end":263},"obj":"VBN"},{"id":"T19152","span":{"begin":241,"end":252},"obj":"NN"},{"id":"T19151","span":{"begin":236,"end":240},"obj":"IN"},{"id":"T19150","span":{"begin":228,"end":235},"obj":"NNS"},{"id":"T19149","span":{"begin":224,"end":227},"obj":"DT"},{"id":"T19148","span":{"begin":221,"end":223},"obj":"IN"},{"id":"T19147","span":{"begin":209,"end":220},"obj":"NNS"},{"id":"T19146","span":{"begin":205,"end":208},"obj":"IN"},{"id":"T19145","span":{"begin":196,"end":199},"obj":"VBD"},{"id":"T19144","span":{"begin":200,"end":204},"obj":"VBN"},{"id":"T19143","span":{"begin":193,"end":194},"obj":"HYPH"},{"id":"T19142","span":{"begin":194,"end":195},"obj":"NN"},{"id":"T19141","span":{"begin":190,"end":193},"obj":"NN"},{"id":"T19140","span":{"begin":189,"end":398},"obj":"sentence"},{"id":"T19139","span":{"begin":188,"end":189},"obj":"."},{"id":"T19138","span":{"begin":178,"end":188},"obj":"NNS"},{"id":"T19137","span":{"begin":174,"end":177},"obj":"CC"},{"id":"T19136","span":{"begin":166,"end":173},"obj":"NNS"},{"id":"T19135","span":{"begin":161,"end":165},"obj":"IN"},{"id":"T19134","span":{"begin":151,"end":160},"obj":"NN"},{"id":"T19133","span":{"begin":148,"end":150},"obj":"IN"},{"id":"T19132","span":{"begin":142,"end":147},"obj":"JJ"},{"id":"T19131","span":{"begin":140,"end":141},"obj":"-RRB-"},{"id":"T19130","span":{"begin":138,"end":139},"obj":"HYPH"},{"id":"T19129","span":{"begin":139,"end":140},"obj":"NN"},{"id":"T19128","span":{"begin":135,"end":138},"obj":"NN"},{"id":"T19127","span":{"begin":134,"end":135},"obj":"-LRB-"},{"id":"T19126","span":{"begin":131,"end":133},"obj":"CD"},{"id":"T19125","span":{"begin":130,"end":131},"obj":"HYPH"},{"id":"T19124","span":{"begin":125,"end":130},"obj":"NN"},{"id":"T19123","span":{"begin":114,"end":124},"obj":"VBG"},{"id":"T19122","span":{"begin":110,"end":113},"obj":"NN"},{"id":"T19121","span":{"begin":105,"end":109},"obj":"IN"},{"id":"T19120","span":{"begin":98,"end":104},"obj":"VBN"},{"id":"T19119","span":{"begin":94,"end":97},"obj":"CC"},{"id":"T19118","span":{"begin":92,"end":94},"obj":","},{"id":"T19117","span":{"begin":84,"end":92},"obj":"NN"},{"id":"T19116","span":{"begin":79,"end":83},"obj":"IN"},{"id":"T19115","span":{"begin":71,"end":78},"obj":"VBN"},{"id":"T19114","span":{"begin":69,"end":71},"obj":","},{"id":"T19113","span":{"begin":53,"end":69},"obj":"NN"},{"id":"T19112","span":{"begin":50,"end":52},"obj":"IN"},{"id":"T19363","span":{"begin":1172,"end":1176},"obj":"DT"},{"id":"T19362","span":{"begin":1167,"end":1171},"obj":"IN"},{"id":"T19361","span":{"begin":1163,"end":1166},"obj":"CD"},{"id":"T19360","span":{"begin":1161,"end":1163},"obj":","},{"id":"T19359","span":{"begin":1148,"end":1152},"obj":"VBD"},{"id":"T19358","span":{"begin":1153,"end":1161},"obj":"VBN"},{"id":"T19357","span":{"begin":1134,"end":1135},"obj":"HYPH"},{"id":"T19356","span":{"begin":1135,"end":1140},"obj":"NN"},{"id":"T19355","span":{"begin":1133,"end":1134},"obj":"NN"},{"id":"T19354","span":{"begin":1141,"end":1147},"obj":"NNS"},{"id":"T19353","span":{"begin":1129,"end":1132},"obj":"CD"},{"id":"T19352","span":{"begin":1128,"end":1211},"obj":"sentence"},{"id":"T19351","span":{"begin":1127,"end":1128},"obj":"."},{"id":"T19350","span":{"begin":1125,"end":1127},"obj":"PRP"},{"id":"T19349","span":{"begin":1122,"end":1124},"obj":"IN"},{"id":"T19348","span":{"begin":1111,"end":1121},"obj":"JJ"},{"id":"T19347","span":{"begin":1105,"end":1110},"obj":"NN"},{"id":"T19346","span":{"begin":1103,"end":1104},"obj":"DT"},{"id":"T19345","span":{"begin":1100,"end":1102},"obj":"IN"},{"id":"T19344","span":{"begin":1095,"end":1099},"obj":"VBD"},{"id":"T19343","span":{"begin":1091,"end":1094},"obj":"CC"},{"id":"T19342","span":{"begin":1084,"end":1090},"obj":"NN"},{"id":"T19341","span":{"begin":1080,"end":1083},"obj":"DT"},{"id":"T19340","span":{"begin":1077,"end":1079},"obj":"IN"},{"id":"T19339","span":{"begin":1069,"end":1076},"obj":"NN"},{"id":"T19338","span":{"begin":1065,"end":1068},"obj":"DT"},{"id":"T19337","span":{"begin":1062,"end":1064},"obj":"IN"},{"id":"T19336","span":{"begin":1056,"end":1061},"obj":"VBD"},{"id":"T19335","span":{"begin":1047,"end":1055},"obj":"NNS"},{"id":"T19334","span":{"begin":1043,"end":1046},"obj":"DT"},{"id":"T19333","span":{"begin":1042,"end":1128},"obj":"sentence"},{"id":"T19332","span":{"begin":1041,"end":1042},"obj":"."},{"id":"T19331","span":{"begin":1039,"end":1041},"obj":"NN"},{"id":"T19330","span":{"begin":1030,"end":1035},"obj":"RBS"},{"id":"T19329","span":{"begin":1036,"end":1038},"obj":"CD"},{"id":"T19328","span":{"begin":1027,"end":1029},"obj":"RB"},{"id":"T19327","span":{"begin":1024,"end":1026},"obj":"IN"},{"id":"T19326","span":{"begin":1018,"end":1023},"obj":"NN"},{"id":"T19325","span":{"begin":1016,"end":1017},"obj":"DT"},{"id":"T19324","span":{"begin":1013,"end":1015},"obj":"IN"},{"id":"T19323","span":{"begin":1008,"end":1009},"obj":"CD"},{"id":"T19322","span":{"begin":1010,"end":1012},"obj":"NN"},{"id":"T19321","span":{"begin":1002,"end":1007},"obj":"DT"},{"id":"T19320","span":{"begin":988,"end":992},"obj":"VBD"},{"id":"T19319","span":{"begin":982,"end":987},"obj":"NN"},{"id":"T19318","span":{"begin":979,"end":981},"obj":"NN"},{"id":"T19317","span":{"begin":977,"end":978},"obj":"CD"},{"id":"T19316","span":{"begin":963,"end":976},"obj":"RB"},{"id":"T19315","span":{"begin":993,"end":1001},"obj":"VBN"},{"id":"T19314","span":{"begin":954,"end":962},"obj":"NNS"},{"id":"T19313","span":{"begin":946,"end":953},"obj":"JJ"},{"id":"T19312","span":{"begin":945,"end":1042},"obj":"sentence"},{"id":"T19311","span":{"begin":944,"end":945},"obj":"."},{"id":"T19310","span":{"begin":943,"end":944},"obj":"-RRB-"},{"id":"T19309","span":{"begin":941,"end":943},"obj":"NN"},{"id":"T19308","span":{"begin":937,"end":940},"obj":"CD"},{"id":"T19307","span":{"begin":933,"end":936},"obj":"CC"},{"id":"T19306","span":{"begin":926,"end":929},"obj":"CD"},{"id":"T19305","span":{"begin":930,"end":932},"obj":"NN"},{"id":"T19304","span":{"begin":925,"end":926},"obj":"-LRB-"},{"id":"T19303","span":{"begin":918,"end":924},"obj":"NNS"},{"id":"T19302","span":{"begin":913,"end":917},"obj":"NN"},{"id":"T19301","span":{"begin":909,"end":912},"obj":"CD"},{"id":"T19300","span":{"begin":905,"end":908},"obj":"CC"},{"id":"T19299","span":{"begin":903,"end":904},"obj":"-RRB-"},{"id":"T19298","span":{"begin":897,"end":900},"obj":"CD"},{"id":"T19297","span":{"begin":901,"end":903},"obj":"NN"},{"id":"T19296","span":{"begin":896,"end":897},"obj":"-LRB-"},{"id":"T19295","span":{"begin":890,"end":895},"obj":"NN"},{"id":"T19294","span":{"begin":887,"end":889},"obj":"CD"},{"id":"T19293","span":{"begin":882,"end":886},"obj":"IN"},{"id":"T19292","span":{"begin":873,"end":881},"obj":"VBN"},{"id":"T19291","span":{"begin":871,"end":872},"obj":"-RRB-"},{"id":"T19290","span":{"begin":868,"end":871},"obj":"NNP"},{"id":"T19289","span":{"begin":866,"end":868},"obj":","},{"id":"T19288","span":{"begin":862,"end":866},"obj":"NNP"},{"id":"T19287","span":{"begin":858,"end":861},"obj":"NNP"},{"id":"T19286","span":{"begin":856,"end":858},"obj":","},{"id":"T19285","span":{"begin":847,"end":856},"obj":"NNP"},{"id":"T19284","span":{"begin":845,"end":847},"obj":","},{"id":"T19283","span":{"begin":835,"end":839},"obj":"NNP"},{"id":"T19282","span":{"begin":840,"end":845},"obj":"NNP"},{"id":"T19281","span":{"begin":834,"end":835},"obj":"-LRB-"},{"id":"T19280","span":{"begin":814,"end":822},"obj":"NN"},{"id":"T19279","span":{"begin":808,"end":813},"obj":"NN"},{"id":"T19278","span":{"begin":823,"end":833},"obj":"NN"},{"id":"T19277","span":{"begin":806,"end":807},"obj":"DT"},{"id":"T19276","span":{"begin":801,"end":805},"obj":"IN"},{"id":"T19275","span":{"begin":789,"end":793},"obj":"VBD"},{"id":"T19274","span":{"begin":794,"end":800},"obj":"VBN"},{"id":"T19273","span":{"begin":781,"end":788},"obj":"NNS"},{"id":"T19272","span":{"begin":777,"end":780},"obj":"DT"},{"id":"T19271","span":{"begin":776,"end":945},"obj":"sentence"},{"id":"T19270","span":{"begin":775,"end":776},"obj":"."},{"id":"T19269","span":{"begin":774,"end":775},"obj":"-RRB-"},{"id":"T19268","span":{"begin":771,"end":774},"obj":"NNP"},{"id":"T19267","span":{"begin":769,"end":771},"obj":","},{"id":"T19266","span":{"begin":767,"end":769},"obj":"NNP"},{"id":"T19265","span":{"begin":765,"end":767},"obj":","},{"id":"T19264","span":{"begin":759,"end":765},"obj":"NNP"},{"id":"T19263","span":{"begin":757,"end":759},"obj":","},{"id":"T19262","span":{"begin":741,"end":750},"obj":"NNP"},{"id":"T19261","span":{"begin":751,"end":757},"obj":"NNP"},{"id":"T19260","span":{"begin":740,"end":741},"obj":"-LRB-"},{"id":"T19259","span":{"begin":720,"end":728},"obj":"JJ"},{"id":"T19258","span":{"begin":708,"end":709},"obj":"HYPH"},{"id":"T19257","span":{"begin":705,"end":708},"obj":"CD"},{"id":"T19256","span":{"begin":709,"end":719},"obj":"VBN"},{"id":"T19255","span":{"begin":699,"end":704},"obj":"NNP"},{"id":"T19254","span":{"begin":695,"end":698},"obj":"CC"},{"id":"T19253","span":{"begin":729,"end":739},"obj":"NNS"},{"id":"T19111","span":{"begin":44,"end":49},"obj":"VBN"},{"id":"T19110","span":{"begin":42,"end":44},"obj":","},{"id":"T19109","span":{"begin":28,"end":32},"obj":"VBD"},{"id":"T19108","span":{"begin":33,"end":42},"obj":"VBN"},{"id":"T19107","span":{"begin":20,"end":27},"obj":"NNS"},{"id":"T19106","span":{"begin":19,"end":189},"obj":"sentence"},{"id":"T19105","span":{"begin":9,"end":19},"obj":"NN"},{"id":"T19104","span":{"begin":0,"end":8},"obj":"JJ"},{"id":"T19390","span":{"begin":1316,"end":1317},"obj":"."},{"id":"T19389","span":{"begin":1302,"end":1310},"obj":"JJ"},{"id":"T19388","span":{"begin":1311,"end":1316},"obj":"NN"},{"id":"T19387","span":{"begin":1298,"end":1301},"obj":"DT"},{"id":"T19386","span":{"begin":1295,"end":1297},"obj":"IN"},{"id":"T19385","span":{"begin":1282,"end":1289},"obj":"VBN"},{"id":"T19384","span":{"begin":1290,"end":1294},"obj":"NN"},{"id":"T19383","span":{"begin":1280,"end":1281},"obj":"DT"},{"id":"T19382","span":{"begin":1273,"end":1279},"obj":"IN"},{"id":"T19381","span":{"begin":1260,"end":1264},"obj":"VBD"},{"id":"T19380","span":{"begin":1252,"end":1259},"obj":"NN"},{"id":"T19379","span":{"begin":1244,"end":1251},"obj":"NN"},{"id":"T19378","span":{"begin":1241,"end":1243},"obj":"IN"},{"id":"T19377","span":{"begin":1230,"end":1240},"obj":"VBN"},{"id":"T19376","span":{"begin":1265,"end":1272},"obj":"VBN"},{"id":"T19375","span":{"begin":1225,"end":1229},"obj":"NNS"},{"id":"T19374","span":{"begin":1221,"end":1224},"obj":"NN"},{"id":"T19373","span":{"begin":1212,"end":1220},"obj":"JJ"},{"id":"T19372","span":{"begin":1211,"end":1317},"obj":"sentence"},{"id":"T19371","span":{"begin":1210,"end":1211},"obj":"."},{"id":"T19370","span":{"begin":1204,"end":1210},"obj":"NN"},{"id":"T19369","span":{"begin":1199,"end":1203},"obj":"DT"},{"id":"T19368","span":{"begin":1196,"end":1198},"obj":"IN"},{"id":"T19367","span":{"begin":1184,"end":1187},"obj":"CD"},{"id":"T19366","span":{"begin":1188,"end":1195},"obj":"NNS"},{"id":"T19365","span":{"begin":1180,"end":1183},"obj":"DT"},{"id":"T19364","span":{"begin":1177,"end":1179},"obj":"IN"}],"relations":[{"id":"R5320","pred":"amod","subj":"T19104","obj":"T19105"},{"id":"R5321","pred":"nsubj","subj":"T19107","obj":"T19108"},{"id":"R5322","pred":"aux","subj":"T19109","obj":"T19108"},{"id":"R5323","pred":"punct","subj":"T19110","obj":"T19108"},{"id":"R5324","pred":"conj","subj":"T19111","obj":"T19108"},{"id":"R5325","pred":"prep","subj":"T19112","obj":"T19111"},{"id":"R5326","pred":"pobj","subj":"T19113","obj":"T19112"},{"id":"R5327","pred":"punct","subj":"T19114","obj":"T19111"},{"id":"R5328","pred":"conj","subj":"T19115","obj":"T19111"},{"id":"R5329","pred":"prep","subj":"T19116","obj":"T19115"},{"id":"R5330","pred":"pobj","subj":"T19117","obj":"T19116"},{"id":"R5331","pred":"punct","subj":"T19118","obj":"T19115"},{"id":"R5332","pred":"cc","subj":"T19119","obj":"T19115"},{"id":"R5333","pred":"conj","subj":"T19120","obj":"T19115"},{"id":"R5334","pred":"prep","subj":"T19121","obj":"T19120"},{"id":"R5335","pred":"pobj","subj":"T19122","obj":"T19121"},{"id":"R5336","pred":"acl","subj":"T19123","obj":"T19122"},{"id":"R5337","pred":"dobj","subj":"T19124","obj":"T19123"},{"id":"R5338","pred":"punct","subj":"T19125","obj":"T19124"},{"id":"R5339","pred":"nummod","subj":"T19126","obj":"T19124"},{"id":"R5340","pred":"punct","subj":"T19127","obj":"T19122"},{"id":"R5341","pred":"compound","subj":"T19128","obj":"T19129"},{"id":"R5342","pred":"appos","subj":"T19129","obj":"T19122"},{"id":"R5343","pred":"punct","subj":"T19130","obj":"T19129"},{"id":"R5344","pred":"punct","subj":"T19131","obj":"T19108"},{"id":"R5345","pred":"amod","subj":"T19132","obj":"T19133"},{"id":"R5346","pred":"prep","subj":"T19133","obj":"T19108"},{"id":"R5347","pred":"pobj","subj":"T19134","obj":"T19133"},{"id":"R5348","pred":"prep","subj":"T19135","obj":"T19134"},{"id":"R5349","pred":"pobj","subj":"T19136","obj":"T19135"},{"id":"R5350","pred":"cc","subj":"T19137","obj":"T19136"},{"id":"R5351","pred":"conj","subj":"T19138","obj":"T19136"},{"id":"R5352","pred":"punct","subj":"T19139","obj":"T19108"},{"id":"R5353","pred":"compound","subj":"T19141","obj":"T19142"},{"id":"R5354","pred":"nsubjpass","subj":"T19142","obj":"T19144"},{"id":"R5355","pred":"punct","subj":"T19143","obj":"T19142"},{"id":"R5356","pred":"auxpass","subj":"T19145","obj":"T19144"},{"id":"R5357","pred":"prep","subj":"T19146","obj":"T19144"},{"id":"R5358","pred":"pobj","subj":"T19147","obj":"T19146"},{"id":"R5359","pred":"prep","subj":"T19148","obj":"T19147"},{"id":"R5360","pred":"det","subj":"T19149","obj":"T19150"},{"id":"R5361","pred":"pobj","subj":"T19150","obj":"T19148"},{"id":"R5362","pred":"prep","subj":"T19151","obj":"T19147"},{"id":"R5363","pred":"npadvmod","subj":"T19152","obj":"T19153"},{"id":"R5364","pred":"amod","subj":"T19153","obj":"T19155"},{"id":"R5365","pred":"punct","subj":"T19154","obj":"T19153"},{"id":"R5366","pred":"pobj","subj":"T19155","obj":"T19151"},{"id":"R5367","pred":"compound","subj":"T19156","obj":"T19155"},{"id":"R5368","pred":"compound","subj":"T19157","obj":"T19155"},{"id":"R5369","pred":"punct","subj":"T19158","obj":"T19155"},{"id":"R5370","pred":"appos","subj":"T19159","obj":"T19155"},{"id":"R5371","pred":"punct","subj":"T19160","obj":"T19155"},{"id":"R5372","pred":"parataxis","subj":"T19161","obj":"T19155"},{"id":"R5373","pred":"punct","subj":"T19162","obj":"T19161"},{"id":"R5374","pred":"npadvmod","subj":"T19163","obj":"T19161"},{"id":"R5375","pred":"punct","subj":"T19164","obj":"T19161"},{"id":"R5376","pred":"npadvmod","subj":"T19165","obj":"T19161"},{"id":"R5377","pred":"punct","subj":"T19166","obj":"T19161"},{"id":"R5378","pred":"punct","subj":"T19167","obj":"T19144"},{"id":"R5379","pred":"cc","subj":"T19168","obj":"T19144"},{"id":"R5380","pred":"compound","subj":"T19169","obj":"T19170"},{"id":"R5381","pred":"nsubjpass","subj":"T19170","obj":"T19172"},{"id":"R5382","pred":"punct","subj":"T19171","obj":"T19170"},{"id":"R5383","pred":"conj","subj":"T19172","obj":"T19144"},{"id":"R5384","pred":"cc","subj":"T19173","obj":"T19170"},{"id":"R5385","pred":"nummod","subj":"T19174","obj":"T19175"},{"id":"R5386","pred":"compound","subj":"T19175","obj":"T19176"},{"id":"R5387","pred":"conj","subj":"T19176","obj":"T19170"},{"id":"R5388","pred":"compound","subj":"T19177","obj":"T19176"},{"id":"R5389","pred":"auxpass","subj":"T19178","obj":"T19172"},{"id":"R5390","pred":"prep","subj":"T19179","obj":"T19172"},{"id":"R5391","pred":"pobj","subj":"T19180","obj":"T19179"},{"id":"R5392","pred":"prep","subj":"T19181","obj":"T19180"},{"id":"R5393","pred":"det","subj":"T19182","obj":"T19183"},{"id":"R5394","pred":"pobj","subj":"T19183","obj":"T19181"},{"id":"R5395","pred":"punct","subj":"T19184","obj":"T19172"},{"id":"R5396","pred":"det","subj":"T19186","obj":"T19187"},{"id":"R5397","pred":"nsubj","subj":"T19187","obj":"T19189"},{"id":"R5398","pred":"amod","subj":"T19188","obj":"T19187"},{"id":"R5399","pred":"attr","subj":"T19190","obj":"T19189"},{"id":"R5400","pred":"punct","subj":"T19191","obj":"T19192"},{"id":"R5401","pred":"parataxis","subj":"T19192","obj":"T19190"},{"id":"R5402","pred":"dep","subj":"T19193","obj":"T19192"},{"id":"R5403","pred":"punct","subj":"T19194","obj":"T19193"},{"id":"R5404","pred":"nummod","subj":"T19195","obj":"T19193"},{"id":"R5405","pred":"punct","subj":"T19196","obj":"T19192"},{"id":"R5406","pred":"compound","subj":"T19197","obj":"T19198"},{"id":"R5407","pred":"compound","subj":"T19198","obj":"T19192"},{"id":"R5408","pred":"punct","subj":"T19199","obj":"T19192"},{"id":"R5409","pred":"compound","subj":"T19200","obj":"T19201"},{"id":"R5410","pred":"npadvmod","subj":"T19201","obj":"T19192"},{"id":"R5411","pred":"punct","subj":"T19202","obj":"T19192"},{"id":"R5412","pred":"npadvmod","subj":"T19203","obj":"T19192"},{"id":"R5413","pred":"punct","subj":"T19204","obj":"T19192"},{"id":"R5414","pred":"npadvmod","subj":"T19205","obj":"T19192"},{"id":"R5415","pred":"punct","subj":"T19206","obj":"T19192"},{"id":"R5416","pred":"punct","subj":"T19207","obj":"T19190"},{"id":"R5417","pred":"conj","subj":"T19208","obj":"T19190"},{"id":"R5418","pred":"punct","subj":"T19209","obj":"T19208"},{"id":"R5419","pred":"compound","subj":"T19210","obj":"T19211"},{"id":"R5420","pred":"appos","subj":"T19211","obj":"T19208"},{"id":"R5421","pred":"punct","subj":"T19212","obj":"T19211"},{"id":"R5422","pred":"punct","subj":"T19213","obj":"T19208"},{"id":"R5423","pred":"appos","subj":"T19214","obj":"T19208"},{"id":"R5424","pred":"punct","subj":"T19215","obj":"T19216"},{"id":"R5425","pred":"parataxis","subj":"T19216","obj":"T19208"},{"id":"R5426","pred":"compound","subj":"T19217","obj":"T19216"},{"id":"R5427","pred":"punct","subj":"T19218","obj":"T19216"},{"id":"R5428","pred":"compound","subj":"T19219","obj":"T19220"},{"id":"R5429","pred":"npadvmod","subj":"T19220","obj":"T19216"},{"id":"R5430","pred":"punct","subj":"T19221","obj":"T19220"},{"id":"R5431","pred":"npadvmod","subj":"T19222","obj":"T19216"},{"id":"R5432","pred":"punct","subj":"T19223","obj":"T19216"},{"id":"R5433","pred":"npadvmod","subj":"T19224","obj":"T19216"},{"id":"R5434","pred":"punct","subj":"T19225","obj":"T19216"},{"id":"R5435","pred":"punct","subj":"T19226","obj":"T19208"},{"id":"R5436","pred":"cc","subj":"T19227","obj":"T19208"},{"id":"R5437","pred":"conj","subj":"T19228","obj":"T19208"},{"id":"R5438","pred":"punct","subj":"T19229","obj":"T19230"},{"id":"R5439","pred":"parataxis","subj":"T19230","obj":"T19228"},{"id":"R5440","pred":"compound","subj":"T19231","obj":"T19230"},{"id":"R5441","pred":"punct","subj":"T19232","obj":"T19230"},{"id":"R5442","pred":"compound","subj":"T19233","obj":"T19230"},{"id":"R5443","pred":"compound","subj":"T19234","obj":"T19230"},{"id":"R5444","pred":"punct","subj":"T19235","obj":"T19230"},{"id":"R5445","pred":"npadvmod","subj":"T19236","obj":"T19230"},{"id":"R5446","pred":"punct","subj":"T19237","obj":"T19230"},{"id":"R5447","pred":"npadvmod","subj":"T19238","obj":"T19230"},{"id":"R5448","pred":"punct","subj":"T19239","obj":"T19230"},{"id":"R5449","pred":"npadvmod","subj":"T19240","obj":"T19230"},{"id":"R5450","pred":"punct","subj":"T19241","obj":"T19230"},{"id":"R5451","pred":"punct","subj":"T19242","obj":"T19189"},{"id":"R5452","pred":"det","subj":"T19244","obj":"T19245"},{"id":"R5453","pred":"nsubj","subj":"T19245","obj":"T19247"},{"id":"R5454","pred":"amod","subj":"T19246","obj":"T19245"},{"id":"R5455","pred":"npadvmod","subj":"T19248","obj":"T19249"},{"id":"R5456","pred":"amod","subj":"T19249","obj":"T19252"},{"id":"R5457","pred":"nummod","subj":"T19250","obj":"T19248"},{"id":"R5458","pred":"punct","subj":"T19251","obj":"T19249"},{"id":"R5459","pred":"nmod","subj":"T19252","obj":"T19253"},{"id":"R5460","pred":"attr","subj":"T19253","obj":"T19247"},{"id":"R5461","pred":"cc","subj":"T19254","obj":"T19252"},{"id":"R5462","pred":"npadvmod","subj":"T19255","obj":"T19256"},{"id":"R5463","pred":"amod","subj":"T19256","obj":"T19259"},{"id":"R5464","pred":"nummod","subj":"T19257","obj":"T19255"},{"id":"R5465","pred":"punct","subj":"T19258","obj":"T19256"},{"id":"R5466","pred":"conj","subj":"T19259","obj":"T19252"},{"id":"R5467","pred":"punct","subj":"T19260","obj":"T19261"},{"id":"R5468","pred":"parataxis","subj":"T19261","obj":"T19253"},{"id":"R5469","pred":"compound","subj":"T19262","obj":"T19261"},{"id":"R5470","pred":"punct","subj":"T19263","obj":"T19261"},{"id":"R5471","pred":"npadvmod","subj":"T19264","obj":"T19261"},{"id":"R5472","pred":"punct","subj":"T19265","obj":"T19261"},{"id":"R5473","pred":"npadvmod","subj":"T19266","obj":"T19261"},{"id":"R5474","pred":"punct","subj":"T19267","obj":"T19261"},{"id":"R5475","pred":"npadvmod","subj":"T19268","obj":"T19261"},{"id":"R5476","pred":"punct","subj":"T19269","obj":"T19261"},{"id":"R5477","pred":"punct","subj":"T19270","obj":"T19247"},{"id":"R5478","pred":"det","subj":"T19272","obj":"T19273"},{"id":"R5479","pred":"nsubjpass","subj":"T19273","obj":"T19274"},{"id":"R5480","pred":"auxpass","subj":"T19275","obj":"T19274"},{"id":"R5481","pred":"prep","subj":"T19276","obj":"T19274"},{"id":"R5482","pred":"det","subj":"T19277","obj":"T19278"},{"id":"R5483","pred":"pobj","subj":"T19278","obj":"T19276"},{"id":"R5484","pred":"compound","subj":"T19279","obj":"T19278"},{"id":"R5485","pred":"compound","subj":"T19280","obj":"T19278"},{"id":"R5486","pred":"punct","subj":"T19281","obj":"T19282"},{"id":"R5487","pred":"parataxis","subj":"T19282","obj":"T19278"},{"id":"R5488","pred":"compound","subj":"T19283","obj":"T19282"},{"id":"R5489","pred":"punct","subj":"T19284","obj":"T19282"},{"id":"R5490","pred":"npadvmod","subj":"T19285","obj":"T19282"},{"id":"R5491","pred":"punct","subj":"T19286","obj":"T19282"},{"id":"R5492","pred":"compound","subj":"T19287","obj":"T19288"},{"id":"R5493","pred":"npadvmod","subj":"T19288","obj":"T19282"},{"id":"R5494","pred":"punct","subj":"T19289","obj":"T19282"},{"id":"R5495","pred":"npadvmod","subj":"T19290","obj":"T19282"},{"id":"R5496","pred":"punct","subj":"T19291","obj":"T19282"},{"id":"R5497","pred":"acl","subj":"T19292","obj":"T19278"},{"id":"R5498","pred":"prep","subj":"T19293","obj":"T19292"},{"id":"R5499","pred":"nummod","subj":"T19294","obj":"T19295"},{"id":"R5500","pred":"pobj","subj":"T19295","obj":"T19293"},{"id":"R5501","pred":"punct","subj":"T19296","obj":"T19297"},{"id":"R5502","pred":"parataxis","subj":"T19297","obj":"T19295"},{"id":"R5503","pred":"nummod","subj":"T19298","obj":"T19297"},{"id":"R5504","pred":"punct","subj":"T19299","obj":"T19297"},{"id":"R5505","pred":"cc","subj":"T19300","obj":"T19295"},{"id":"R5506","pred":"nummod","subj":"T19301","obj":"T19302"},{"id":"R5507","pred":"compound","subj":"T19302","obj":"T19303"},{"id":"R5508","pred":"conj","subj":"T19303","obj":"T19295"},{"id":"R5509","pred":"punct","subj":"T19304","obj":"T19305"},{"id":"R5510","pred":"parataxis","subj":"T19305","obj":"T19303"},{"id":"R5511","pred":"nummod","subj":"T19306","obj":"T19305"},{"id":"R5512","pred":"cc","subj":"T19307","obj":"T19305"},{"id":"R5513","pred":"nummod","subj":"T19308","obj":"T19309"},{"id":"R5514","pred":"conj","subj":"T19309","obj":"T19305"},{"id":"R5515","pred":"punct","subj":"T19310","obj":"T19305"},{"id":"R5516","pred":"punct","subj":"T19311","obj":"T19274"},{"id":"R5517","pred":"amod","subj":"T19313","obj":"T19314"},{"id":"R5518","pred":"nsubjpass","subj":"T19314","obj":"T19315"},{"id":"R5519","pred":"advmod","subj":"T19316","obj":"T19317"},{"id":"R5520","pred":"nummod","subj":"T19317","obj":"T19318"},{"id":"R5521","pred":"compound","subj":"T19318","obj":"T19319"},{"id":"R5522","pred":"amod","subj":"T19319","obj":"T19314"},{"id":"R5523","pred":"auxpass","subj":"T19320","obj":"T19315"},{"id":"R5524","pred":"det","subj":"T19321","obj":"T19322"},{"id":"R5525","pred":"npadvmod","subj":"T19322","obj":"T19315"},{"id":"R5526","pred":"nummod","subj":"T19323","obj":"T19322"},{"id":"R5527","pred":"prep","subj":"T19324","obj":"T19315"},{"id":"R5528","pred":"det","subj":"T19325","obj":"T19326"},{"id":"R5529","pred":"pobj","subj":"T19326","obj":"T19324"},{"id":"R5530","pred":"prep","subj":"T19327","obj":"T19326"},{"id":"R5531","pred":"advmod","subj":"T19328","obj":"T19329"},{"id":"R5532","pred":"nummod","subj":"T19329","obj":"T19331"},{"id":"R5533","pred":"advmod","subj":"T19330","obj":"T19329"},{"id":"R5534","pred":"pobj","subj":"T19331","obj":"T19327"},{"id":"R5535","pred":"punct","subj":"T19332","obj":"T19315"},{"id":"R5536","pred":"det","subj":"T19334","obj":"T19335"},{"id":"R5537","pred":"nsubj","subj":"T19335","obj":"T19336"},{"id":"R5538","pred":"prep","subj":"T19337","obj":"T19336"},{"id":"R5539","pred":"det","subj":"T19338","obj":"T19339"},{"id":"R5540","pred":"pobj","subj":"T19339","obj":"T19337"},{"id":"R5541","pred":"prep","subj":"T19340","obj":"T19339"},{"id":"R5542","pred":"det","subj":"T19341","obj":"T19342"},{"id":"R5543","pred":"pobj","subj":"T19342","obj":"T19340"},{"id":"R5544","pred":"cc","subj":"T19343","obj":"T19336"},{"id":"R5545","pred":"conj","subj":"T19344","obj":"T19336"},{"id":"R5546","pred":"prep","subj":"T19345","obj":"T19344"},{"id":"R5547","pred":"det","subj":"T19346","obj":"T19347"},{"id":"R5548","pred":"pobj","subj":"T19347","obj":"T19345"},{"id":"R5549","pred":"amod","subj":"T19348","obj":"T19347"},{"id":"R5550","pred":"prep","subj":"T19349","obj":"T19348"},{"id":"R5551","pred":"pobj","subj":"T19350","obj":"T19349"},{"id":"R5552","pred":"punct","subj":"T19351","obj":"T19336"},{"id":"R5553","pred":"nummod","subj":"T19353","obj":"T19354"},{"id":"R5554","pred":"nsubjpass","subj":"T19354","obj":"T19358"},{"id":"R5555","pred":"compound","subj":"T19355","obj":"T19356"},{"id":"R5556","pred":"compound","subj":"T19356","obj":"T19354"},{"id":"R5557","pred":"punct","subj":"T19357","obj":"T19356"},{"id":"R5558","pred":"auxpass","subj":"T19359","obj":"T19358"},{"id":"R5559","pred":"punct","subj":"T19360","obj":"T19358"},{"id":"R5560","pred":"dobj","subj":"T19361","obj":"T19358"},{"id":"R5561","pred":"prep","subj":"T19362","obj":"T19361"},{"id":"R5562","pred":"pobj","subj":"T19363","obj":"T19362"},{"id":"R5563","pred":"prep","subj":"T19364","obj":"T19363"},{"id":"R5564","pred":"det","subj":"T19365","obj":"T19366"},{"id":"R5565","pred":"pobj","subj":"T19366","obj":"T19364"},{"id":"R5566","pred":"nummod","subj":"T19367","obj":"T19366"},{"id":"R5567","pred":"prep","subj":"T19368","obj":"T19366"},{"id":"R5568","pred":"det","subj":"T19369","obj":"T19370"},{"id":"R5569","pred":"pobj","subj":"T19370","obj":"T19368"},{"id":"R5570","pred":"punct","subj":"T19371","obj":"T19358"},{"id":"R5571","pred":"amod","subj":"T19373","obj":"T19374"},{"id":"R5572","pred":"compound","subj":"T19374","obj":"T19375"},{"id":"R5573","pred":"nsubjpass","subj":"T19375","obj":"T19376"},{"id":"R5574","pred":"acl","subj":"T19377","obj":"T19375"},{"id":"R5575","pred":"prep","subj":"T19378","obj":"T19377"},{"id":"R5576","pred":"compound","subj":"T19379","obj":"T19380"},{"id":"R5577","pred":"pobj","subj":"T19380","obj":"T19378"},{"id":"R5578","pred":"auxpass","subj":"T19381","obj":"T19376"},{"id":"R5579","pred":"prep","subj":"T19382","obj":"T19376"},{"id":"R5580","pred":"det","subj":"T19383","obj":"T19384"},{"id":"R5581","pred":"pobj","subj":"T19384","obj":"T19382"},{"id":"R5582","pred":"amod","subj":"T19385","obj":"T19384"},{"id":"R5583","pred":"prep","subj":"T19386","obj":"T19384"},{"id":"R5584","pred":"det","subj":"T19387","obj":"T19388"},{"id":"R5585","pred":"pobj","subj":"T19388","obj":"T19386"},{"id":"R5586","pred":"amod","subj":"T19389","obj":"T19388"},{"id":"R5587","pred":"punct","subj":"T19390","obj":"T19376"}],"text":"Confocal microscopy\nKidneys were dissected, fixed in paraformaldehyde, treated with methanol, and washed with PBS containing Tween-20 (PBS-T) prior to treatment with lectins and antibodies. PBS-T was used for incubations of the tissues with fluoroscein-conjugated Dilichos biflorus aggutinin (DBA, Vector; Burlingame, USA), and PBS-T plus 2% goat serum was used for incubations with the antibodies. The primary antibodies were anti-WT1 (c-19; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-uvomorulin (e-cadherin, Cdh1, Sigma-Aldrich St. Louis, MO, USA), and anti-Cited1 (Neomarkers-Lab Vision Corporation, Fremont, CA, USA). The secondary antibodies were Alexa 555-conjugated anti-rabbit and Alexa 633-conjugated anti-rat antibodies (Molecular Probes, Eugene, OR, USA).\nThe tissues were imaged with a laser scanning microscope (Carl Zeiss, Thornwood, New York, USA) equipped with an argon (488 nm) and two HeNe lasers (543 nm and 633 nm). Optical sections approximately 2 μm thick were obtained every 5 μm to a depth of at least 65 μm. The sections began at the surface of the kidney and were on a plane tangential to it. Two Z-stack series were obtained, one from each of the two kidneys of each embryo. Ureteric bud tips identified by section tracing were counted within a defined area of the confocal image."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T19093","span":{"begin":709,"end":719},"obj":"MOP:0000779"},{"id":"T19092","span":{"begin":688,"end":694},"obj":"NCBITaxon:9986"},{"id":"T19091","span":{"begin":672,"end":682},"obj":"MOP:0000779"},{"id":"T19090","span":{"begin":662,"end":671},"obj":"CHEBI:52673"},{"id":"T19089","span":{"begin":646,"end":656},"obj":"GO:0042571"},{"id":"T19088","span":{"begin":570,"end":576},"obj":"PR_EXT:000005505"},{"id":"T19087","span":{"begin":520,"end":524},"obj":"PR_EXT:000001447"},{"id":"T19086","span":{"begin":508,"end":518},"obj":"PR_EXT:000001447"},{"id":"T19085","span":{"begin":496,"end":506},"obj":"PR_EXT:000001447"},{"id":"T19084","span":{"begin":432,"end":435},"obj":"PR_EXT:000017459"},{"id":"T19083","span":{"begin":411,"end":421},"obj":"GO:0042571"},{"id":"T19082","span":{"begin":387,"end":397},"obj":"GO:0042571"},{"id":"T19081","span":{"begin":347,"end":352},"obj":"UBERON:0001977"},{"id":"T19080","span":{"begin":342,"end":346},"obj":"NCBITaxon:9925"},{"id":"T19079","span":{"begin":264,"end":281},"obj":"NCBITaxon:271171"},{"id":"T19078","span":{"begin":253,"end":263},"obj":"MOP:0000779"},{"id":"T19077","span":{"begin":241,"end":252},"obj":"CHEBI:31624"},{"id":"T19076","span":{"begin":228,"end":235},"obj":"UBERON:0000479"},{"id":"T19075","span":{"begin":194,"end":195},"obj":"CHEBI:53424"},{"id":"T19074","span":{"begin":178,"end":188},"obj":"GO:0042571"},{"id":"T19073","span":{"begin":139,"end":140},"obj":"CHEBI:53424"},{"id":"T19072","span":{"begin":125,"end":133},"obj":"CHEBI:53424"},{"id":"T19071","span":{"begin":84,"end":92},"obj":"CHEBI:17790"},{"id":"T19070","span":{"begin":53,"end":69},"obj":"CHEBI_EXT:paraformaldehye"},{"id":"T19069","span":{"begin":20,"end":27},"obj":"UBERON:0002113"},{"id":"T19103","span":{"begin":1212,"end":1229},"obj":"UBERON:0006364"},{"id":"T19102","span":{"begin":1204,"end":1210},"obj":"UBERON:0000922"},{"id":"T19101","span":{"begin":1188,"end":1195},"obj":"UBERON:0002113"},{"id":"T19100","span":{"begin":1084,"end":1090},"obj":"UBERON:0002113"},{"id":"T19099","span":{"begin":890,"end":895},"obj":"CHEBI_EXT:argon"},{"id":"T19098","span":{"begin":781,"end":788},"obj":"UBERON:0000479"},{"id":"T19097","span":{"begin":751,"end":757},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T19096","span":{"begin":741,"end":750},"obj":"CHEBI_EXT:polyatomic_entity_or_group"},{"id":"T19095","span":{"begin":729,"end":739},"obj":"GO:0042571"},{"id":"T19094","span":{"begin":725,"end":728},"obj":"NCBITaxon:10114"}],"text":"Confocal microscopy\nKidneys were dissected, fixed in paraformaldehyde, treated with methanol, and washed with PBS containing Tween-20 (PBS-T) prior to treatment with lectins and antibodies. PBS-T was used for incubations of the tissues with fluoroscein-conjugated Dilichos biflorus aggutinin (DBA, Vector; Burlingame, USA), and PBS-T plus 2% goat serum was used for incubations with the antibodies. The primary antibodies were anti-WT1 (c-19; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-uvomorulin (e-cadherin, Cdh1, Sigma-Aldrich St. Louis, MO, USA), and anti-Cited1 (Neomarkers-Lab Vision Corporation, Fremont, CA, USA). The secondary antibodies were Alexa 555-conjugated anti-rabbit and Alexa 633-conjugated anti-rat antibodies (Molecular Probes, Eugene, OR, USA).\nThe tissues were imaged with a laser scanning microscope (Carl Zeiss, Thornwood, New York, USA) equipped with an argon (488 nm) and two HeNe lasers (543 nm and 633 nm). Optical sections approximately 2 μm thick were obtained every 5 μm to a depth of at least 65 μm. The sections began at the surface of the kidney and were on a plane tangential to it. Two Z-stack series were obtained, one from each of the two kidneys of each embryo. Ureteric bud tips identified by section tracing were counted within a defined area of the confocal image."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T19068","span":{"begin":1212,"end":1229},"obj":"UBERON:0006364"},{"id":"T19067","span":{"begin":1204,"end":1210},"obj":"UBERON:0000922"},{"id":"T19066","span":{"begin":1188,"end":1195},"obj":"UBERON:0002113"},{"id":"T19065","span":{"begin":1084,"end":1090},"obj":"UBERON:0002113"},{"id":"T19064","span":{"begin":781,"end":788},"obj":"UBERON:0000479"},{"id":"T19063","span":{"begin":729,"end":739},"obj":"GO:0042571"},{"id":"T19062","span":{"begin":725,"end":728},"obj":"NCBITaxon:10114"},{"id":"T19061","span":{"begin":709,"end":719},"obj":"MOP:0000779"},{"id":"T19060","span":{"begin":688,"end":694},"obj":"NCBITaxon:9986"},{"id":"T19059","span":{"begin":672,"end":682},"obj":"MOP:0000779"},{"id":"T19058","span":{"begin":662,"end":671},"obj":"CHEBI:52673"},{"id":"T19057","span":{"begin":646,"end":656},"obj":"GO:0042571"},{"id":"T19056","span":{"begin":570,"end":576},"obj":"PR:000005505"},{"id":"T19055","span":{"begin":520,"end":524},"obj":"PR:000001447"},{"id":"T19054","span":{"begin":508,"end":518},"obj":"PR:000001447"},{"id":"T19053","span":{"begin":496,"end":506},"obj":"PR:000001447"},{"id":"T19052","span":{"begin":432,"end":435},"obj":"PR:000017459"},{"id":"T19051","span":{"begin":411,"end":421},"obj":"GO:0042571"},{"id":"T19050","span":{"begin":387,"end":397},"obj":"GO:0042571"},{"id":"T19049","span":{"begin":347,"end":352},"obj":"UBERON:0001977"},{"id":"T19048","span":{"begin":342,"end":346},"obj":"NCBITaxon:9925"},{"id":"T19047","span":{"begin":264,"end":281},"obj":"NCBITaxon:271171"},{"id":"T19046","span":{"begin":253,"end":263},"obj":"MOP:0000779"},{"id":"T19045","span":{"begin":241,"end":252},"obj":"CHEBI:31624"},{"id":"T19044","span":{"begin":228,"end":235},"obj":"UBERON:0000479"},{"id":"T19043","span":{"begin":194,"end":195},"obj":"CHEBI:53424"},{"id":"T19042","span":{"begin":178,"end":188},"obj":"GO:0042571"},{"id":"T19041","span":{"begin":139,"end":140},"obj":"CHEBI:53424"},{"id":"T19040","span":{"begin":125,"end":133},"obj":"CHEBI:53424"},{"id":"T19039","span":{"begin":84,"end":92},"obj":"CHEBI:17790"},{"id":"T19038","span":{"begin":20,"end":27},"obj":"UBERON:0002113"}],"text":"Confocal microscopy\nKidneys were dissected, fixed in paraformaldehyde, treated with methanol, and washed with PBS containing Tween-20 (PBS-T) prior to treatment with lectins and antibodies. PBS-T was used for incubations of the tissues with fluoroscein-conjugated Dilichos biflorus aggutinin (DBA, Vector; Burlingame, USA), and PBS-T plus 2% goat serum was used for incubations with the antibodies. The primary antibodies were anti-WT1 (c-19; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-uvomorulin (e-cadherin, Cdh1, Sigma-Aldrich St. Louis, MO, USA), and anti-Cited1 (Neomarkers-Lab Vision Corporation, Fremont, CA, USA). The secondary antibodies were Alexa 555-conjugated anti-rabbit and Alexa 633-conjugated anti-rat antibodies (Molecular Probes, Eugene, OR, USA).\nThe tissues were imaged with a laser scanning microscope (Carl Zeiss, Thornwood, New York, USA) equipped with an argon (488 nm) and two HeNe lasers (543 nm and 633 nm). Optical sections approximately 2 μm thick were obtained every 5 μm to a depth of at least 65 μm. The sections began at the surface of the kidney and were on a plane tangential to it. Two Z-stack series were obtained, one from each of the two kidneys of each embryo. Ureteric bud tips identified by section tracing were counted within a defined area of the confocal image."}