Id |
Subject |
Object |
Predicate |
Lexical cue |
T27371 |
513-524 |
VB |
denotes |
demonstrate |
T27370 |
510-512 |
TO |
denotes |
to |
T27369 |
496-500 |
VBD |
denotes |
were |
T27368 |
501-509 |
VBN |
denotes |
included |
T27367 |
483-488 |
NN |
denotes |
Pygo2 |
T27366 |
479-482 |
CC |
denotes |
and |
T27365 |
489-495 |
NNS |
denotes |
probes |
T27364 |
473-478 |
NN |
denotes |
Pygo1 |
T27363 |
472-566 |
sentence |
denotes |
Pygo1 and Pygo2 probes were included to demonstrate significant changes in expression levels. |
T27362 |
471-472 |
. |
denotes |
. |
T27361 |
464-471 |
NNS |
denotes |
samples |
T27360 |
456-461 |
RBS |
denotes |
least |
T27359 |
462-463 |
CD |
denotes |
3 |
T27358 |
453-455 |
RB |
denotes |
at |
T27357 |
450-452 |
IN |
denotes |
in |
T27356 |
446-449 |
CD |
denotes |
100 |
T27355 |
445-446 |
SYM |
denotes |
> |
T27354 |
442-444 |
VB |
denotes |
be |
T27353 |
439-441 |
TO |
denotes |
to |
T27352 |
418-428 |
NN |
denotes |
expression |
T27351 |
414-417 |
JJ |
denotes |
raw |
T27350 |
429-438 |
NN |
denotes |
intensity |
T27349 |
410-413 |
DT |
denotes |
the |
T27348 |
400-409 |
VBG |
denotes |
requiring |
T27347 |
382-387 |
NN |
denotes |
level |
T27346 |
371-381 |
NN |
denotes |
expression |
T27345 |
388-399 |
NN |
denotes |
restriction |
T27344 |
368-370 |
DT |
denotes |
an |
T27343 |
362-367 |
VBG |
denotes |
using |
T27342 |
356-361 |
NNS |
denotes |
genes |
T27341 |
353-355 |
CD |
denotes |
40 |
T27340 |
350-352 |
IN |
denotes |
of |
T27339 |
344-349 |
NN |
denotes |
total |
T27338 |
342-343 |
DT |
denotes |
a |
T27337 |
339-341 |
IN |
denotes |
to |
T27336 |
331-338 |
VBN |
denotes |
reduced |
T27335 |
326-330 |
RB |
denotes |
then |
T27334 |
322-325 |
CC |
denotes |
and |
T27333 |
310-313 |
VBD |
denotes |
was |
T27332 |
298-301 |
NN |
denotes |
Wnt |
T27331 |
302-309 |
NNS |
denotes |
targets |
T27330 |
295-297 |
CD |
denotes |
82 |
T27329 |
292-294 |
IN |
denotes |
of |
T27328 |
314-321 |
VBN |
denotes |
created |
T27327 |
282-286 |
NN |
denotes |
gene |
T27326 |
274-281 |
JJ |
denotes |
initial |
T27325 |
287-291 |
NN |
denotes |
list |
T27324 |
271-273 |
DT |
denotes |
An |
T27323 |
270-472 |
sentence |
denotes |
An initial gene list of 82 Wnt targets was created and then reduced to a total of 40 genes using an expression level restriction requiring the raw expression intensity to be >100 in at least 3 samples. |
T27322 |
269-270 |
. |
denotes |
. |
T27321 |
268-269 |
-RRB- |
denotes |
] |
T27320 |
266-268 |
CD |
denotes |
39 |
T27319 |
265-266 |
-LRB- |
denotes |
[ |
T27318 |
262-264 |
IN |
denotes |
at |
T27317 |
253-261 |
VBN |
denotes |
compiled |
T27316 |
247-252 |
DT |
denotes |
those |
T27310 |
207-215 |
JJ |
denotes |
Possible |
T27309 |
206-270 |
sentence |
denotes |
Possible Wnt targets were selected from those compiled at [39]. |
T27308 |
205-206 |
. |
denotes |
. |
T27307 |
192-197 |
NN |
denotes |
E18.5 |
T27306 |
178-186 |
NN |
denotes |
compound |
T27305 |
176-177 |
CD |
denotes |
2 |
T27304 |
175-176 |
HYPH |
denotes |
/ |
T27303 |
187-191 |
JJ |
denotes |
null |
T27302 |
170-175 |
NN |
denotes |
Pygo1 |
T27301 |
166-169 |
CC |
denotes |
and |
T27300 |
198-205 |
NNS |
denotes |
kidneys |
T27299 |
160-161 |
HYPH |
denotes |
- |
T27298 |
161-165 |
NN |
denotes |
type |
T27297 |
156-160 |
JJ |
denotes |
wild |
T27296 |
153-155 |
IN |
denotes |
on |
T27295 |
142-152 |
NN |
denotes |
triplicate |
T27294 |
139-141 |
IN |
denotes |
in |
T27293 |
125-128 |
VBD |
denotes |
was |
T27292 |
129-138 |
VBN |
denotes |
performed |
T27291 |
116-124 |
NN |
denotes |
analysis |
T27290 |
105-115 |
NN |
denotes |
Microarray |
T27289 |
104-206 |
sentence |
denotes |
Microarray analysis was performed in triplicate on wild-type and Pygo1/2 compound null E18.5 kidneys. |
T27288 |
103-104 |
. |
denotes |
. |
T27287 |
92-96 |
JJ |
denotes |
null |
T27286 |
85-86 |
HYPH |
denotes |
/ |
T27285 |
86-91 |
NN |
denotes |
Pygo2 |
T27284 |
80-85 |
NN |
denotes |
Pygo1 |
T27283 |
74-79 |
NN |
denotes |
E18.5 |
T27282 |
97-103 |
NN |
denotes |
kidney |
T27281 |
70-73 |
DT |
denotes |
the |
T27280 |
67-69 |
IN |
denotes |
in |
T27279 |
49-58 |
NN |
denotes |
signaling |
T27278 |
45-48 |
NN |
denotes |
Wnt |
T27277 |
59-66 |
NNS |
denotes |
targets |
T27276 |
38-44 |
JJ |
denotes |
common |
T27275 |
35-37 |
IN |
denotes |
of |
T27274 |
27-34 |
NNS |
denotes |
changes |
T27273 |
16-26 |
NN |
denotes |
expression |
T27272 |
11-15 |
NN |
denotes |
Gene |
T27315 |
242-246 |
IN |
denotes |
from |
T27314 |
228-232 |
VBD |
denotes |
were |
T27313 |
233-241 |
VBN |
denotes |
selected |
T27312 |
216-219 |
NN |
denotes |
Wnt |
T27311 |
220-227 |
NNS |
denotes |
targets |
T27377 |
565-566 |
. |
denotes |
. |
T27376 |
559-565 |
NNS |
denotes |
levels |
T27375 |
548-558 |
NN |
denotes |
expression |
T27374 |
545-547 |
IN |
denotes |
in |
T27373 |
537-544 |
NNS |
denotes |
changes |
T27372 |
525-536 |
JJ |
denotes |
significant |
T12614 |
575-587 |
JJ |
denotes |
quantitative |
T12613 |
570-574 |
VBD |
denotes |
used |
T12612 |
567-569 |
PRP |
denotes |
We |
T12611 |
0-675 |
sentence |
denotes |
Figure 8 Gene expression changes of common Wnt signaling targets in the E18.5 Pygo1/Pygo2 null kidney. Microarray analysis was performed in triplicate on wild-type and Pygo1/2 compound null E18.5 kidneys. Possible Wnt targets were selected from those compiled at [39]. An initial gene list of 82 Wnt targets was created and then reduced to a total of 40 genes using an expression level restriction requiring the raw expression intensity to be >100 in at least 3 samples. Pygo1 and Pygo2 probes were included to demonstrate significant changes in expression levels. We used quantitative real-time PCR, with independent biological samples, to validate the microarray results. |
T12630 |
674-675 |
. |
denotes |
. |
T12629 |
656-666 |
NN |
denotes |
microarray |
T12628 |
667-674 |
NNS |
denotes |
results |
T12627 |
652-655 |
DT |
denotes |
the |
T12626 |
643-651 |
VB |
denotes |
validate |
T12625 |
640-642 |
TO |
denotes |
to |
T12624 |
638-640 |
, |
denotes |
, |
T12623 |
620-630 |
JJ |
denotes |
biological |
T12622 |
631-638 |
NNS |
denotes |
samples |
T12621 |
608-619 |
JJ |
denotes |
independent |
T12620 |
603-607 |
IN |
denotes |
with |
T12619 |
601-603 |
, |
denotes |
, |
T12618 |
592-593 |
HYPH |
denotes |
- |
T12617 |
593-597 |
NN |
denotes |
time |
T12616 |
588-592 |
JJ |
denotes |
real |
T12615 |
598-601 |
NN |
denotes |
PCR |
R7885 |
T27272 |
T27273 |
compound |
Gene,expression |
R7886 |
T27273 |
T27274 |
compound |
expression,changes |
R7887 |
T27275 |
T27274 |
prep |
of,changes |
R7888 |
T27276 |
T27277 |
amod |
common,targets |
R7889 |
T27277 |
T27275 |
pobj |
targets,of |
R7890 |
T27278 |
T27277 |
compound |
Wnt,targets |
R7891 |
T27279 |
T27277 |
compound |
signaling,targets |
R7892 |
T27280 |
T27274 |
prep |
in,changes |
R7893 |
T27281 |
T27282 |
det |
the,kidney |
R7894 |
T27282 |
T27280 |
pobj |
kidney,in |
R7895 |
T27283 |
T27282 |
nmod |
E18.5,kidney |
R7896 |
T27284 |
T27285 |
compound |
Pygo1,Pygo2 |
R7897 |
T27285 |
T27287 |
npadvmod |
Pygo2,null |
R7898 |
T27286 |
T27285 |
punct |
/,Pygo2 |
R7899 |
T27287 |
T27282 |
amod |
null,kidney |
R7900 |
T27288 |
T27274 |
punct |
.,changes |
R7901 |
T27290 |
T27291 |
compound |
Microarray,analysis |
R7902 |
T27291 |
T27292 |
nsubjpass |
analysis,performed |
R7903 |
T27293 |
T27292 |
auxpass |
was,performed |
R7904 |
T27294 |
T27292 |
prep |
in,performed |
R7905 |
T27295 |
T27294 |
pobj |
triplicate,in |
R7906 |
T27296 |
T27292 |
prep |
on,performed |
R7907 |
T27297 |
T27298 |
amod |
wild,type |
R7908 |
T27298 |
T27300 |
nmod |
type,kidneys |
R7909 |
T27299 |
T27298 |
punct |
-,type |
R7910 |
T27300 |
T27296 |
pobj |
kidneys,on |
R7911 |
T27301 |
T27298 |
cc |
and,type |
R7912 |
T27302 |
T27303 |
npadvmod |
Pygo1,null |
R7913 |
T27303 |
T27298 |
conj |
null,type |
R7914 |
T27304 |
T27302 |
punct |
/,Pygo1 |
R7915 |
T27305 |
T27302 |
nummod |
2,Pygo1 |
R7916 |
T27306 |
T27303 |
npadvmod |
compound,null |
R7917 |
T27307 |
T27300 |
compound |
E18.5,kidneys |
R7918 |
T27308 |
T27292 |
punct |
.,performed |
R7919 |
T27310 |
T27311 |
amod |
Possible,targets |
R7920 |
T27311 |
T27313 |
nsubjpass |
targets,selected |
R7921 |
T27312 |
T27311 |
compound |
Wnt,targets |
R7922 |
T27314 |
T27313 |
auxpass |
were,selected |
R7923 |
T27315 |
T27313 |
prep |
from,selected |
R7924 |
T27316 |
T27315 |
pobj |
those,from |
R7925 |
T27317 |
T27316 |
acl |
compiled,those |
R7926 |
T27318 |
T27317 |
prep |
at,compiled |
R7927 |
T27319 |
T27318 |
punct |
[,at |
R7928 |
T27320 |
T27318 |
pobj |
39,at |
R7929 |
T27321 |
T27313 |
punct |
],selected |
R7930 |
T27322 |
T27313 |
punct |
.,selected |
R7931 |
T27324 |
T27325 |
det |
An,list |
R7932 |
T27325 |
T27328 |
nsubjpass |
list,created |
R7933 |
T27326 |
T27325 |
amod |
initial,list |
R7934 |
T27327 |
T27325 |
compound |
gene,list |
R7935 |
T27329 |
T27325 |
prep |
of,list |
R7936 |
T27330 |
T27331 |
nummod |
82,targets |
R7937 |
T27331 |
T27329 |
pobj |
targets,of |
R7938 |
T27332 |
T27331 |
compound |
Wnt,targets |
R7939 |
T27333 |
T27328 |
auxpass |
was,created |
R7940 |
T27334 |
T27328 |
cc |
and,created |
R7941 |
T27335 |
T27336 |
advmod |
then,reduced |
R7942 |
T27336 |
T27328 |
conj |
reduced,created |
R7943 |
T27337 |
T27336 |
prep |
to,reduced |
R7944 |
T27338 |
T27339 |
det |
a,total |
R7945 |
T27339 |
T27337 |
pobj |
total,to |
R7946 |
T27340 |
T27339 |
prep |
of,total |
R7947 |
T27341 |
T27342 |
nummod |
40,genes |
R7948 |
T27342 |
T27340 |
pobj |
genes,of |
R7949 |
T27343 |
T27336 |
advcl |
using,reduced |
R7950 |
T27344 |
T27345 |
det |
an,restriction |
R7951 |
T27345 |
T27343 |
dobj |
restriction,using |
R7952 |
T27346 |
T27347 |
compound |
expression,level |
R7953 |
T27347 |
T27345 |
compound |
level,restriction |
R7954 |
T27348 |
T27345 |
acl |
requiring,restriction |
R7955 |
T27349 |
T27350 |
det |
the,intensity |
R7956 |
T27350 |
T27348 |
dobj |
intensity,requiring |
R7957 |
T27351 |
T27350 |
amod |
raw,intensity |
R7958 |
T27352 |
T27350 |
compound |
expression,intensity |
R7959 |
T27353 |
T27354 |
aux |
to,be |
R7960 |
T27354 |
T27348 |
xcomp |
be,requiring |
R7961 |
T27355 |
T27356 |
punct |
>,100 |
R7962 |
T27356 |
T27354 |
attr |
100,be |
R7963 |
T27357 |
T27354 |
prep |
in,be |
R7964 |
T27358 |
T27359 |
advmod |
at,3 |
R7965 |
T27359 |
T27361 |
nummod |
3,samples |
R7966 |
T27360 |
T27359 |
advmod |
least,3 |
R7967 |
T27361 |
T27357 |
pobj |
samples,in |
R7968 |
T27362 |
T27328 |
punct |
.,created |
R7969 |
T27364 |
T27365 |
nmod |
Pygo1,probes |
R7970 |
T27365 |
T27368 |
nsubjpass |
probes,included |
R7971 |
T27366 |
T27364 |
cc |
and,Pygo1 |
R7972 |
T27367 |
T27364 |
conj |
Pygo2,Pygo1 |
R7973 |
T27369 |
T27368 |
auxpass |
were,included |
R7974 |
T27370 |
T27371 |
aux |
to,demonstrate |
R7975 |
T27371 |
T27368 |
advcl |
demonstrate,included |
R7976 |
T27372 |
T27373 |
amod |
significant,changes |
R7977 |
T27373 |
T27371 |
dobj |
changes,demonstrate |
R7978 |
T27374 |
T27373 |
prep |
in,changes |
R7979 |
T27375 |
T27376 |
compound |
expression,levels |
R7980 |
T27376 |
T27374 |
pobj |
levels,in |
R7981 |
T27377 |
T27368 |
punct |
.,included |
R3545 |
T12612 |
T12613 |
nsubj |
We,used |
R3546 |
T12614 |
T12615 |
amod |
quantitative,PCR |
R3547 |
T12615 |
T12613 |
dobj |
PCR,used |
R3548 |
T12616 |
T12617 |
amod |
real,time |
R3549 |
T12617 |
T12615 |
compound |
time,PCR |
R3550 |
T12618 |
T12617 |
punct |
-,time |
R3551 |
T12619 |
T12615 |
punct |
", ",PCR |
R3552 |
T12620 |
T12615 |
prep |
with,PCR |
R3553 |
T12621 |
T12622 |
amod |
independent,samples |
R3554 |
T12622 |
T12620 |
pobj |
samples,with |
R3555 |
T12623 |
T12622 |
amod |
biological,samples |
R3556 |
T12624 |
T12613 |
punct |
", ",used |
R3557 |
T12625 |
T12626 |
aux |
to,validate |
R3558 |
T12626 |
T12613 |
advcl |
validate,used |
R3559 |
T12627 |
T12628 |
det |
the,results |
R3560 |
T12628 |
T12626 |
dobj |
results,validate |
R3561 |
T12629 |
T12628 |
compound |
microarray,results |
R3562 |
T12630 |
T12613 |
punct |
.,used |