Id |
Subject |
Object |
Predicate |
Lexical cue |
T27371 |
5407-5418 |
VB |
denotes |
demonstrate |
T27370 |
5404-5406 |
TO |
denotes |
to |
T27369 |
5390-5394 |
VBD |
denotes |
were |
T27368 |
5395-5403 |
VBN |
denotes |
included |
T27367 |
5377-5382 |
NN |
denotes |
Pygo2 |
T27366 |
5373-5376 |
CC |
denotes |
and |
T27365 |
5383-5389 |
NNS |
denotes |
probes |
T27364 |
5367-5372 |
NN |
denotes |
Pygo1 |
T27363 |
5366-5460 |
sentence |
denotes |
Pygo1 and Pygo2 probes were included to demonstrate significant changes in expression levels. |
T27362 |
5365-5366 |
. |
denotes |
. |
T27361 |
5358-5365 |
NNS |
denotes |
samples |
T27360 |
5350-5355 |
RBS |
denotes |
least |
T27359 |
5356-5357 |
CD |
denotes |
3 |
T27358 |
5347-5349 |
RB |
denotes |
at |
T27357 |
5344-5346 |
IN |
denotes |
in |
T27356 |
5340-5343 |
CD |
denotes |
100 |
T27355 |
5339-5340 |
SYM |
denotes |
> |
T27354 |
5336-5338 |
VB |
denotes |
be |
T27353 |
5333-5335 |
TO |
denotes |
to |
T27352 |
5312-5322 |
NN |
denotes |
expression |
T27351 |
5308-5311 |
JJ |
denotes |
raw |
T27350 |
5323-5332 |
NN |
denotes |
intensity |
T27349 |
5304-5307 |
DT |
denotes |
the |
T27348 |
5294-5303 |
VBG |
denotes |
requiring |
T27347 |
5276-5281 |
NN |
denotes |
level |
T27346 |
5265-5275 |
NN |
denotes |
expression |
T27345 |
5282-5293 |
NN |
denotes |
restriction |
T27344 |
5262-5264 |
DT |
denotes |
an |
T27343 |
5256-5261 |
VBG |
denotes |
using |
T27342 |
5250-5255 |
NNS |
denotes |
genes |
T27341 |
5247-5249 |
CD |
denotes |
40 |
T27340 |
5244-5246 |
IN |
denotes |
of |
T27339 |
5238-5243 |
NN |
denotes |
total |
T27338 |
5236-5237 |
DT |
denotes |
a |
T27337 |
5233-5235 |
IN |
denotes |
to |
T27336 |
5225-5232 |
VBN |
denotes |
reduced |
T27335 |
5220-5224 |
RB |
denotes |
then |
T27334 |
5216-5219 |
CC |
denotes |
and |
T27333 |
5204-5207 |
VBD |
denotes |
was |
T27332 |
5192-5195 |
NN |
denotes |
Wnt |
T27331 |
5196-5203 |
NNS |
denotes |
targets |
T27330 |
5189-5191 |
CD |
denotes |
82 |
T27329 |
5186-5188 |
IN |
denotes |
of |
T27328 |
5208-5215 |
VBN |
denotes |
created |
T27327 |
5176-5180 |
NN |
denotes |
gene |
T27326 |
5168-5175 |
JJ |
denotes |
initial |
T27325 |
5181-5185 |
NN |
denotes |
list |
T27324 |
5165-5167 |
DT |
denotes |
An |
T27323 |
5164-5366 |
sentence |
denotes |
An initial gene list of 82 Wnt targets was created and then reduced to a total of 40 genes using an expression level restriction requiring the raw expression intensity to be >100 in at least 3 samples. |
T27322 |
5163-5164 |
. |
denotes |
. |
T27321 |
5162-5163 |
-RRB- |
denotes |
] |
T27320 |
5160-5162 |
CD |
denotes |
39 |
T27319 |
5159-5160 |
-LRB- |
denotes |
[ |
T27318 |
5156-5158 |
IN |
denotes |
at |
T27317 |
5147-5155 |
VBN |
denotes |
compiled |
T27316 |
5141-5146 |
DT |
denotes |
those |
T27589 |
1444-1449 |
NN |
denotes |
Pygo2 |
T27588 |
1438-1443 |
NN |
denotes |
Pygo1 |
T27587 |
1461-1467 |
NN |
denotes |
kidney |
T27586 |
1434-1437 |
DT |
denotes |
the |
T27585 |
1431-1433 |
IN |
denotes |
in |
T27584 |
1429-1430 |
-RRB- |
denotes |
) |
T27583 |
1422-1429 |
JJR |
denotes |
greater |
T27582 |
1419-1421 |
CC |
denotes |
or |
T27581 |
1403-1411 |
JJ |
denotes |
two-fold |
T27580 |
1401-1403 |
, |
denotes |
, |
T27579 |
1395-1396 |
SYM |
denotes |
< |
T27578 |
1397-1401 |
CD |
denotes |
0.05 |
T27577 |
1393-1394 |
NN |
denotes |
p |
T27576 |
1412-1418 |
NN |
denotes |
change |
T27575 |
1392-1393 |
-LRB- |
denotes |
( |
T27574 |
1382-1391 |
VBN |
denotes |
expressed |
T27573 |
1367-1381 |
RB |
denotes |
differentially |
T27572 |
1361-1366 |
NNS |
denotes |
Genes |
T27310 |
5101-5109 |
JJ |
denotes |
Possible |
T27309 |
5100-5164 |
sentence |
denotes |
Possible Wnt targets were selected from those compiled at [39]. |
T27308 |
5099-5100 |
. |
denotes |
. |
T27307 |
5086-5091 |
NN |
denotes |
E18.5 |
T27306 |
5072-5080 |
NN |
denotes |
compound |
T27305 |
5070-5071 |
CD |
denotes |
2 |
T27304 |
5069-5070 |
HYPH |
denotes |
/ |
T27303 |
5081-5085 |
JJ |
denotes |
null |
T27302 |
5064-5069 |
NN |
denotes |
Pygo1 |
T27301 |
5060-5063 |
CC |
denotes |
and |
T27300 |
5092-5099 |
NNS |
denotes |
kidneys |
T27299 |
5054-5055 |
HYPH |
denotes |
- |
T27298 |
5055-5059 |
NN |
denotes |
type |
T27297 |
5050-5054 |
JJ |
denotes |
wild |
T27296 |
5047-5049 |
IN |
denotes |
on |
T27295 |
5036-5046 |
NN |
denotes |
triplicate |
T27294 |
5033-5035 |
IN |
denotes |
in |
T27293 |
5019-5022 |
VBD |
denotes |
was |
T27292 |
5023-5032 |
VBN |
denotes |
performed |
T27291 |
5010-5018 |
NN |
denotes |
analysis |
T27290 |
4999-5009 |
NN |
denotes |
Microarray |
T27289 |
4998-5100 |
sentence |
denotes |
Microarray analysis was performed in triplicate on wild-type and Pygo1/2 compound null E18.5 kidneys. |
T27288 |
4997-4998 |
. |
denotes |
. |
T27287 |
4986-4990 |
JJ |
denotes |
null |
T27286 |
4979-4980 |
HYPH |
denotes |
/ |
T27285 |
4980-4985 |
NN |
denotes |
Pygo2 |
T27284 |
4974-4979 |
NN |
denotes |
Pygo1 |
T27283 |
4968-4973 |
NN |
denotes |
E18.5 |
T27282 |
4991-4997 |
NN |
denotes |
kidney |
T27281 |
4964-4967 |
DT |
denotes |
the |
T27280 |
4961-4963 |
IN |
denotes |
in |
T27279 |
4943-4952 |
NN |
denotes |
signaling |
T27278 |
4939-4942 |
NN |
denotes |
Wnt |
T27277 |
4953-4960 |
NNS |
denotes |
targets |
T27276 |
4932-4938 |
JJ |
denotes |
common |
T27275 |
4929-4931 |
IN |
denotes |
of |
T27274 |
4921-4928 |
NNS |
denotes |
changes |
T27273 |
4910-4920 |
NN |
denotes |
expression |
T27272 |
4905-4909 |
NN |
denotes |
Gene |
T12571 |
4673-4677 |
IN |
denotes |
with |
T12570 |
4671-4673 |
, |
denotes |
, |
T12569 |
4650-4663 |
RB |
denotes |
significantly |
T12568 |
4645-4649 |
VBD |
denotes |
were |
T12567 |
4643-4644 |
-RRB- |
denotes |
] |
T12566 |
4641-4643 |
CD |
denotes |
30 |
T12565 |
4640-4641 |
-LRB- |
denotes |
[ |
T12564 |
4634-4639 |
NN |
denotes |
Wisp1 |
T12563 |
4630-4633 |
CC |
denotes |
and |
T12562 |
4628-4630 |
, |
denotes |
, |
T12561 |
4627-4628 |
-RRB- |
denotes |
] |
T12560 |
4625-4627 |
CD |
denotes |
29 |
T12559 |
4624-4625 |
-LRB- |
denotes |
[ |
T12558 |
4614-4620 |
NN |
denotes |
cyclin |
T12557 |
4621-4623 |
NN |
denotes |
D1 |
T12556 |
4605-4613 |
VBG |
denotes |
encoding |
T12555 |
4599-4604 |
NN |
denotes |
Ccnd1 |
T12554 |
4596-4598 |
IN |
denotes |
of |
T12553 |
4664-4671 |
VBN |
denotes |
changed |
T12552 |
4581-4584 |
DT |
denotes |
the |
T12551 |
4585-4595 |
NN |
denotes |
expression |
T12550 |
4576-4580 |
RB |
denotes |
Only |
T12549 |
4575-4744 |
sentence |
denotes |
Only the expression of Ccnd1 encoding cyclin D1 [29], and Wisp1 [30] were significantly changed, with expression level changes of <1.5-fold in Pygo1/Pygo2 null kidneys. |
T12548 |
4574-4575 |
. |
denotes |
. |
T12547 |
4573-4574 |
-RRB- |
denotes |
) |
T12546 |
4572-4573 |
CD |
denotes |
8 |
T12545 |
4565-4571 |
NN |
denotes |
Figure |
T12544 |
4564-4565 |
-LRB- |
denotes |
( |
T12543 |
4549-4555 |
NN |
denotes |
mutant |
T12542 |
4545-4548 |
CC |
denotes |
and |
T12541 |
4556-4563 |
NNS |
denotes |
kidneys |
T12540 |
4539-4540 |
HYPH |
denotes |
- |
T12539 |
4540-4544 |
NN |
denotes |
type |
T12538 |
4535-4539 |
JJ |
denotes |
wild |
T12537 |
4527-4534 |
IN |
denotes |
between |
T12536 |
4516-4526 |
NN |
denotes |
expression |
T12535 |
4513-4515 |
IN |
denotes |
in |
T12534 |
4501-4512 |
NNS |
denotes |
differences |
T12533 |
4497-4500 |
JJ |
denotes |
few |
T12532 |
4492-4496 |
RB |
denotes |
very |
T12531 |
4472-4478 |
NN |
denotes |
target |
T12530 |
4468-4471 |
NN |
denotes |
Wnt |
T12529 |
4462-4467 |
VBN |
denotes |
known |
T12528 |
4479-4484 |
NNS |
denotes |
genes |
T12527 |
4458-4461 |
DT |
denotes |
the |
T12526 |
4456-4458 |
, |
denotes |
, |
T12525 |
4485-4491 |
VBD |
denotes |
showed |
T12524 |
4446-4456 |
RB |
denotes |
Remarkably |
T12523 |
4445-4575 |
sentence |
denotes |
Remarkably, the known Wnt target genes showed very few differences in expression between wild-type and mutant kidneys (Figure 8). |
T12522 |
4444-4445 |
. |
denotes |
. |
T12521 |
4429-4433 |
JJ |
denotes |
down |
T12520 |
4434-4444 |
NN |
denotes |
regulation |
T12519 |
4417-4428 |
JJ |
denotes |
significant |
T12518 |
4406-4416 |
VB |
denotes |
illustrate |
T12517 |
4403-4405 |
TO |
denotes |
to |
T12516 |
4392-4402 |
NNS |
denotes |
references |
T12515 |
4389-4391 |
IN |
denotes |
as |
T12514 |
4375-4379 |
VBD |
denotes |
were |
T12513 |
4380-4388 |
VBN |
denotes |
included |
T12512 |
4364-4369 |
NN |
denotes |
probe |
T12511 |
4358-4363 |
NN |
denotes |
Pygo2 |
T12510 |
4354-4357 |
CC |
denotes |
and |
T12509 |
4370-4374 |
NNS |
denotes |
sets |
T12508 |
4348-4353 |
NN |
denotes |
Pygo1 |
T12507 |
4343-4347 |
CC |
denotes |
Both |
T12506 |
4342-4445 |
sentence |
denotes |
Both Pygo1 and Pygo2 probe sets were included as references to illustrate significant down regulation. |
T12505 |
4341-4342 |
. |
denotes |
. |
T12504 |
4336-4340 |
IN |
denotes |
from |
T12503 |
4323-4326 |
VBD |
denotes |
was |
T12502 |
4311-4314 |
NN |
denotes |
Wnt |
T12501 |
4315-4322 |
NNS |
denotes |
targets |
T12500 |
4305-4310 |
JJ |
denotes |
known |
T12499 |
4302-4304 |
IN |
denotes |
of |
T12498 |
4327-4335 |
VBN |
denotes |
compiled |
T12497 |
4297-4301 |
NN |
denotes |
list |
T12496 |
4295-4296 |
DT |
denotes |
A |
T12495 |
4294-4342 |
sentence |
denotes |
A list of known Wnt targets was compiled from . |
T12494 |
4293-4294 |
. |
denotes |
. |
T12493 |
4277-4278 |
SYM |
denotes |
- |
T12492 |
4276-4277 |
HYPH |
denotes |
/ |
T12491 |
4275-4276 |
SYM |
denotes |
- |
T12490 |
4270-4275 |
NN |
denotes |
Pygo2 |
T12489 |
4269-4270 |
HYPH |
denotes |
/ |
T12488 |
4268-4269 |
SYM |
denotes |
- |
T12487 |
4267-4268 |
HYPH |
denotes |
/ |
T12486 |
4266-4267 |
SYM |
denotes |
- |
T12485 |
4279-4285 |
NN |
denotes |
mutant |
T12484 |
4261-4266 |
NN |
denotes |
Pygo1 |
T12483 |
4286-4293 |
NNS |
denotes |
kidneys |
T12482 |
4257-4260 |
DT |
denotes |
the |
T12481 |
4254-4256 |
IN |
denotes |
in |
T12480 |
4242-4245 |
NN |
denotes |
Wnt |
T12479 |
4246-4253 |
NNS |
denotes |
targets |
T12478 |
4236-4241 |
VBN |
denotes |
known |
T12477 |
4225-4235 |
RB |
denotes |
previously |
T12476 |
4222-4224 |
IN |
denotes |
of |
T12475 |
4203-4210 |
VBN |
denotes |
altered |
T12474 |
4194-4202 |
JJ |
denotes |
possible |
T12473 |
4211-4221 |
NN |
denotes |
expression |
T12472 |
4190-4193 |
DT |
denotes |
the |
T12471 |
4187-4189 |
IN |
denotes |
in |
T12470 |
4176-4186 |
JJ |
denotes |
interested |
T12469 |
4171-4175 |
VBD |
denotes |
were |
T12468 |
4168-4170 |
PRP |
denotes |
We |
T12467 |
4167-4294 |
sentence |
denotes |
We were interested in the possible altered expression of previously known Wnt targets in the Pygo1-/-/Pygo2-/- mutant kidneys. |
T12466 |
4166-4167 |
. |
denotes |
. |
T12465 |
4165-4166 |
-RRB- |
denotes |
] |
T12464 |
4163-4165 |
CD |
denotes |
28 |
T12463 |
4162-4163 |
-LRB- |
denotes |
[ |
T12462 |
4138-4147 |
NN |
denotes |
branching |
T12461 |
4148-4161 |
NN |
denotes |
morphogenesis |
T12460 |
4129-4137 |
JJ |
denotes |
ureteric |
T12459 |
4120-4128 |
VB |
denotes |
regulate |
T12458 |
4117-4119 |
TO |
denotes |
to |
T12457 |
4106-4110 |
VBN |
denotes |
been |
T12456 |
4101-4105 |
RB |
denotes |
also |
T12455 |
4097-4100 |
VBZ |
denotes |
has |
T12454 |
4095-4097 |
, |
denotes |
, |
T12453 |
4082-4090 |
JJ |
denotes |
ureteric |
T12452 |
4072-4081 |
VBG |
denotes |
branching |
T12451 |
4091-4095 |
NNS |
denotes |
tips |
T12450 |
4068-4071 |
DT |
denotes |
the |
T12449 |
4065-4067 |
IN |
denotes |
in |
T12448 |
4055-4064 |
VBN |
denotes |
expressed |
T12447 |
4051-4054 |
CC |
denotes |
and |
T12446 |
4049-4051 |
, |
denotes |
, |
T12445 |
4042-4049 |
NNS |
denotes |
rhythms |
T12444 |
4032-4041 |
JJ |
denotes |
circadian |
T12443 |
4029-4031 |
IN |
denotes |
of |
T12442 |
4018-4028 |
NN |
denotes |
regulation |
T12441 |
4014-4017 |
DT |
denotes |
the |
T12440 |
4011-4013 |
IN |
denotes |
in |
T12439 |
4002-4010 |
VBN |
denotes |
involved |
T12438 |
3981-3994 |
NN |
denotes |
transcription |
T12437 |
3995-4001 |
NN |
denotes |
factor |
T12436 |
3979-3980 |
DT |
denotes |
a |
T12435 |
3977-3979 |
, |
denotes |
, |
T12434 |
4111-4116 |
VBN |
denotes |
shown |
T12398 |
3790-3792 |
, |
denotes |
, |
T12397 |
3771-3772 |
HYPH |
denotes |
- |
T12396 |
3772-3783 |
NN |
denotes |
angiotensin |
T12395 |
3766-3771 |
NN |
denotes |
renin |
T12394 |
3784-3790 |
NN |
denotes |
system |
T12393 |
3762-3765 |
DT |
denotes |
the |
T12392 |
3750-3752 |
, |
denotes |
, |
T12391 |
3749-3750 |
CD |
denotes |
1 |
T12390 |
3743-3748 |
NN |
denotes |
Renin |
T12389 |
3729-3742 |
NN |
denotes |
endopeptidase |
T12388 |
3725-3728 |
DT |
denotes |
the |
T12387 |
3722-3724 |
IN |
denotes |
of |
T12386 |
3714-3721 |
NN |
denotes |
homolog |
T12385 |
3712-3713 |
DT |
denotes |
a |
T12384 |
3710-3712 |
, |
denotes |
, |
T12383 |
3752-3761 |
VBZ |
denotes |
activates |
T12382 |
3706-3710 |
NN |
denotes |
Ren2 |
T12381 |
3705-3823 |
sentence |
denotes |
Ren2, a homolog of the endopeptidase Renin 1, activates the renin-angiotensin system, increasing blood pressure [26]. |
T12380 |
3704-3705 |
. |
denotes |
. |
T12379 |
3703-3704 |
-RRB- |
denotes |
] |
T12378 |
3701-3703 |
CD |
denotes |
25 |
T12377 |
3700-3701 |
-LRB- |
denotes |
[ |
T12376 |
3691-3699 |
NN |
denotes |
pressure |
T12375 |
3685-3690 |
NN |
denotes |
blood |
T12374 |
3682-3684 |
IN |
denotes |
of |
T12373 |
3671-3681 |
NN |
denotes |
regulation |
T12372 |
3667-3670 |
CC |
denotes |
and |
T12371 |
3665-3667 |
, |
denotes |
, |
T12370 |
3655-3665 |
NN |
denotes |
remodeling |
T12369 |
3648-3654 |
NN |
denotes |
tissue |
T12368 |
3646-3648 |
, |
denotes |
, |
T12367 |
3640-3646 |
NN |
denotes |
cancer |
T12366 |
3637-3639 |
IN |
denotes |
in |
T12365 |
3627-3636 |
NNS |
denotes |
functions |
T12364 |
3619-3626 |
JJ |
denotes |
diverse |
T12363 |
3614-3618 |
VBP |
denotes |
have |
T12361 |
3601-3705 |
sentence |
denotes |
Kallikreins have diverse functions in cancer, tissue remodeling, and regulation of blood pressure [25]. |
T12688 |
5869-5870 |
. |
denotes |
. |
T12687 |
5854-5864 |
NN |
denotes |
microarray |
T12686 |
5865-5869 |
NNS |
denotes |
data |
T12685 |
5850-5853 |
DT |
denotes |
the |
T12684 |
5840-5849 |
VBG |
denotes |
screening |
T12683 |
5837-5839 |
IN |
denotes |
in |
T12682 |
5832-5836 |
VBN |
denotes |
used |
T12681 |
5817-5820 |
JJ |
denotes |
low |
T12680 |
5806-5816 |
RB |
denotes |
relatively |
T12679 |
5821-5831 |
NN |
denotes |
stringency |
T12678 |
5802-5805 |
DT |
denotes |
the |
T12677 |
5790-5801 |
VBG |
denotes |
considering |
T12676 |
5788-5790 |
, |
denotes |
, |
T12675 |
5776-5778 |
VB |
denotes |
be |
T12674 |
5772-5775 |
RB |
denotes |
not |
T12673 |
5766-5771 |
MD |
denotes |
could |
T12672 |
5755-5759 |
CD |
denotes |
nine |
T12671 |
5760-5765 |
NNS |
denotes |
genes |
T12670 |
5751-5754 |
DT |
denotes |
the |
T12669 |
5748-5750 |
IN |
denotes |
of |
T12668 |
5744-5747 |
CD |
denotes |
two |
T12667 |
5779-5788 |
VBN |
denotes |
validated |
T12666 |
5739-5743 |
IN |
denotes |
that |
T12665 |
5728-5738 |
JJ |
denotes |
surprising |
T12664 |
5724-5727 |
RB |
denotes |
not |
T12663 |
5721-5723 |
VBZ |
denotes |
is |
T12662 |
5718-5720 |
PRP |
denotes |
It |
T12661 |
5717-5870 |
sentence |
denotes |
It is not surprising that two of the nine genes could not be validated, considering the relatively low stringency used in screening the microarray data. |
T12660 |
5716-5717 |
. |
denotes |
. |
T12659 |
5715-5716 |
-RRB- |
denotes |
) |
T12658 |
5714-5715 |
CD |
denotes |
4 |
T12657 |
5708-5713 |
NN |
denotes |
Table |
T12656 |
5707-5708 |
-LRB- |
denotes |
( |
T12655 |
5688-5698 |
NN |
denotes |
microarray |
T12654 |
5699-5706 |
NNS |
denotes |
results |
T12653 |
5684-5687 |
DT |
denotes |
the |
T12652 |
5681-5683 |
IN |
denotes |
by |
T12360 |
3600-3601 |
. |
denotes |
. |
T12359 |
3584-3590 |
NN |
denotes |
serine |
T12358 |
3591-3600 |
NNS |
denotes |
proteases |
T12357 |
3578-3579 |
HYPH |
denotes |
- |
T12356 |
3579-3583 |
JJ |
denotes |
like |
T12355 |
3571-3578 |
NN |
denotes |
trypsin |
T12354 |
3568-3570 |
IN |
denotes |
of |
T12353 |
3550-3560 |
NN |
denotes |
kallikrein |
T12352 |
3561-3567 |
NN |
denotes |
family |
T12351 |
3546-3549 |
DT |
denotes |
the |
T12350 |
3543-3545 |
IN |
denotes |
of |
T12349 |
3535-3542 |
NNS |
denotes |
members |
T12348 |
3526-3530 |
NN |
denotes |
Klk6 |
T12347 |
3522-3525 |
CC |
denotes |
and |
T12346 |
3531-3534 |
VBP |
denotes |
are |
T12345 |
3517-3521 |
NN |
denotes |
Klk5 |
T12344 |
3516-3601 |
sentence |
denotes |
Klk5 and Klk6 are members of the kallikrein family of trypsin-like serine proteases. |
T12343 |
3515-3516 |
. |
denotes |
. |
T12342 |
3514-3515 |
-RRB- |
denotes |
) |
T12341 |
3506-3514 |
RB |
denotes |
2.4-fold |
T12340 |
3505-3506 |
-LRB- |
denotes |
( |
T12339 |
3496-3504 |
NNP |
denotes |
Timeless |
T12338 |
3492-3495 |
CC |
denotes |
and |
T12337 |
3490-3492 |
, |
denotes |
, |
T12336 |
3489-3490 |
-RRB- |
denotes |
) |
T12335 |
3481-3489 |
RB |
denotes |
2.2-fold |
T12334 |
3480-3481 |
-LRB- |
denotes |
( |
T12333 |
3475-3479 |
NN |
denotes |
Ren2 |
T12332 |
3473-3475 |
, |
denotes |
, |
T12331 |
3472-3473 |
-RRB- |
denotes |
) |
T12330 |
3464-3472 |
RB |
denotes |
2.7-fold |
T12329 |
3463-3464 |
-LRB- |
denotes |
( |
T12328 |
3458-3462 |
NN |
denotes |
Klk6 |
T12327 |
3456-3458 |
, |
denotes |
, |
T12326 |
3455-3456 |
-RRB- |
denotes |
) |
T12325 |
3447-3455 |
RB |
denotes |
2.8-fold |
T12324 |
3446-3447 |
-LRB- |
denotes |
( |
T12323 |
3441-3445 |
NN |
denotes |
Klk5 |
T12322 |
3420-3424 |
JJ |
denotes |
null |
T12321 |
3413-3414 |
HYPH |
denotes |
/ |
T12320 |
3414-3419 |
NN |
denotes |
Pygo2 |
T12319 |
3408-3413 |
NN |
denotes |
Pygo1 |
T12318 |
3425-3431 |
NN |
denotes |
kidney |
T12317 |
3404-3407 |
DT |
denotes |
the |
T12287 |
1235-1236 |
-RRB- |
denotes |
] |
T12286 |
1233-1235 |
CD |
denotes |
24 |
T12285 |
1232-1233 |
-LRB- |
denotes |
[ |
T12284 |
1219-1224 |
JJ |
denotes |
adult |
T12283 |
1225-1231 |
NN |
denotes |
kidney |
T12282 |
1215-1218 |
DT |
denotes |
the |
T12281 |
1212-1214 |
IN |
denotes |
of |
T12280 |
1204-1211 |
NNS |
denotes |
tubules |
T12279 |
1200-1203 |
DT |
denotes |
the |
T12278 |
1197-1199 |
IN |
denotes |
in |
T12277 |
1178-1186 |
RB |
denotes |
strongly |
T12276 |
1173-1177 |
RB |
denotes |
also |
T12275 |
1170-1172 |
VBZ |
denotes |
is |
T12274 |
1168-1170 |
, |
denotes |
, |
T12273 |
1161-1168 |
NN |
denotes |
Slco1a4 |
T12272 |
1159-1161 |
, |
denotes |
, |
T12271 |
1187-1196 |
VBN |
denotes |
expressed |
T12270 |
1142-1147 |
NN |
denotes |
anion |
T12269 |
1134-1141 |
JJ |
denotes |
organic |
T12268 |
1148-1159 |
NN |
denotes |
transporter |
T12267 |
1130-1133 |
DT |
denotes |
The |
T12266 |
1129-1237 |
sentence |
denotes |
The organic anion transporter, Slco1a4, is also strongly expressed in the tubules of the adult kidney [24]. |
T12265 |
1128-1129 |
. |
denotes |
. |
T12264 |
1127-1128 |
-RRB- |
denotes |
] |
T12263 |
1125-1127 |
CD |
denotes |
23 |
T12262 |
1124-1125 |
-LRB- |
denotes |
[ |
T12261 |
1116-1123 |
NN |
denotes |
nephron |
T12260 |
1112-1115 |
DT |
denotes |
the |
T12259 |
1109-1111 |
IN |
denotes |
by |
T12258 |
1102-1108 |
NN |
denotes |
uptake |
T12257 |
1094-1101 |
NN |
denotes |
glucose |
T12256 |
1091-1093 |
IN |
denotes |
of |
T12255 |
1086-1090 |
NN |
denotes |
loss |
T12254 |
1083-1085 |
IN |
denotes |
by |
T12253 |
1069-1082 |
VBN |
denotes |
characterized |
T12252 |
1067-1069 |
, |
denotes |
, |
T12251 |
1051-1056 |
JJ |
denotes |
renal |
T12250 |
1057-1067 |
NN |
denotes |
glucosuria |
T12249 |
1041-1050 |
JJ |
denotes |
recessive |
T12248 |
1031-1040 |
JJ |
denotes |
autosomal |
T12247 |
1028-1030 |
IN |
denotes |
in |
T12246 |
1012-1016 |
VBN |
denotes |
been |
T12245 |
1017-1027 |
VBN |
denotes |
implicated |
T12244 |
1008-1011 |
VBZ |
denotes |
has |
T12243 |
1004-1007 |
CC |
denotes |
and |
T12242 |
990-995 |
JJ |
denotes |
adult |
T12241 |
996-1003 |
NN |
denotes |
nephron |
T12240 |
986-989 |
DT |
denotes |
the |
T12239 |
983-985 |
IN |
denotes |
of |
T12238 |
966-974 |
JJ |
denotes |
proximal |
T12237 |
975-982 |
NNS |
denotes |
tubules |
T12236 |
962-965 |
DT |
denotes |
the |
T12235 |
959-961 |
IN |
denotes |
in |
T12234 |
946-948 |
VBZ |
denotes |
is |
T12233 |
949-958 |
VBN |
denotes |
expressed |
T12232 |
939-945 |
NN |
denotes |
Slc5a2 |
T12231 |
938-1129 |
sentence |
denotes |
Slc5a2 is expressed in the proximal tubules of the adult nephron and has been implicated in autosomal recessive renal glucosuria, characterized by loss of glucose uptake by the nephron [23]. |
T12230 |
937-938 |
. |
denotes |
. |
T12229 |
936-937 |
-RRB- |
denotes |
) |
T12228 |
926-927 |
SYM |
denotes |
- |
T12227 |
928-936 |
RB |
denotes |
2.1-fold |
T12226 |
925-926 |
-LRB- |
denotes |
( |
T12225 |
917-924 |
NN |
denotes |
Slco1a4 |
T12224 |
913-916 |
CC |
denotes |
and |
T12223 |
911-913 |
, |
denotes |
, |
T12222 |
910-911 |
-RRB- |
denotes |
) |
T12221 |
900-901 |
SYM |
denotes |
- |
T12220 |
902-910 |
RB |
denotes |
2.8-fold |
T12219 |
899-900 |
-LRB- |
denotes |
( |
T12218 |
892-898 |
NN |
denotes |
Slc5a2 |
T12217 |
890-892 |
, |
denotes |
, |
T12216 |
889-890 |
-RRB- |
denotes |
) |
T12214 |
881-889 |
RB |
denotes |
2.3-fold |
T12213 |
878-879 |
-LRB- |
denotes |
( |
T12212 |
871-877 |
NN |
denotes |
Cxcl13 |
T12211 |
854-861 |
NNS |
denotes |
kidneys |
T12210 |
847-853 |
NN |
denotes |
mutant |
T12209 |
844-846 |
IN |
denotes |
in |
T12208 |
834-843 |
NNS |
denotes |
decreases |
T12207 |
822-833 |
JJ |
denotes |
significant |
T12206 |
817-821 |
IN |
denotes |
with |
T12205 |
862-870 |
VBD |
denotes |
included |
T12204 |
811-816 |
NNS |
denotes |
genes |
T12203 |
805-810 |
JJ |
denotes |
Other |
T12202 |
804-938 |
sentence |
denotes |
Other genes with significant decreases in mutant kidneys included Cxcl13 (- 2.3-fold), Slc5a2 (- 2.8-fold), and Slco1a4 (- 2.1-fold). |
T12201 |
803-804 |
. |
denotes |
. |
T12200 |
789-795 |
NN |
denotes |
mutant |
T12199 |
796-803 |
NNS |
denotes |
kidneys |
T12198 |
785-788 |
DT |
denotes |
the |
T12197 |
782-784 |
IN |
denotes |
in |
T12196 |
780-781 |
-RRB- |
denotes |
) |
T12195 |
766-768 |
, |
denotes |
, |
T12194 |
758-766 |
RB |
denotes |
3.9-fold |
T12193 |
756-757 |
SYM |
denotes |
- |
T12192 |
752-755 |
CC |
denotes |
and |
T12191 |
743-751 |
RB |
denotes |
5.3-fold |
T12190 |
741-742 |
SYM |
denotes |
- |
T12189 |
768-780 |
RB |
denotes |
respectively |
T12188 |
740-741 |
-LRB- |
denotes |
( |
T12187 |
726-739 |
VBN |
denotes |
downregulated |
T12186 |
723-725 |
IN |
denotes |
as |
T12185 |
707-711 |
VBD |
denotes |
were |
T12184 |
701-706 |
NN |
denotes |
Pygo2 |
T12183 |
697-700 |
CC |
denotes |
and |
T12433 |
3969-3977 |
NN |
denotes |
Timeless |
T12432 |
3968-4167 |
sentence |
denotes |
Timeless, a transcription factor involved in the regulation of circadian rhythms, and expressed in the branching ureteric tips, has also been shown to regulate ureteric branching morphogenesis [28]. |
T12431 |
3967-3968 |
. |
denotes |
. |
T12430 |
3966-3967 |
-RRB- |
denotes |
] |
T12429 |
3964-3966 |
CD |
denotes |
27 |
T12428 |
3963-3964 |
-LRB- |
denotes |
[ |
T12427 |
3956-3962 |
NN |
denotes |
system |
T12426 |
3950-3951 |
HYPH |
denotes |
- |
T12425 |
3951-3955 |
NN |
denotes |
duct |
T12424 |
3940-3950 |
NN |
denotes |
collecting |
T12423 |
3936-3939 |
CC |
denotes |
and |
T12422 |
3929-3935 |
NN |
denotes |
ureter |
T12421 |
3925-3928 |
DT |
denotes |
the |
T12420 |
3922-3924 |
IN |
denotes |
of |
T12419 |
3908-3921 |
NNS |
denotes |
abnormalities |
T12418 |
3897-3907 |
JJ |
denotes |
congenital |
T12417 |
3894-3896 |
IN |
denotes |
in |
T12416 |
3874-3885 |
NN |
denotes |
development |
T12415 |
3867-3873 |
IN |
denotes |
during |
T12414 |
3847-3848 |
HYPH |
denotes |
- |
T12413 |
3848-3859 |
NN |
denotes |
angiotensin |
T12412 |
3842-3847 |
NN |
denotes |
renin |
T12411 |
3860-3866 |
NN |
denotes |
system |
T12410 |
3838-3841 |
DT |
denotes |
the |
T12409 |
3835-3837 |
IN |
denotes |
of |
T12408 |
3886-3893 |
VBZ |
denotes |
results |
T12407 |
3824-3834 |
NN |
denotes |
Disruption |
T12406 |
3823-3968 |
sentence |
denotes |
Disruption of the renin-angiotensin system during development results in congenital abnormalities of the ureter and collecting-duct system [27]. |
T12405 |
3822-3823 |
. |
denotes |
. |
T12404 |
3821-3822 |
-RRB- |
denotes |
] |
T12403 |
3819-3821 |
CD |
denotes |
26 |
T12402 |
3818-3819 |
-LRB- |
denotes |
[ |
T12401 |
3809-3817 |
NN |
denotes |
pressure |
T12400 |
3803-3808 |
NN |
denotes |
blood |
T12399 |
3792-3802 |
VBG |
denotes |
increasing |
T12167 |
647-650 |
CC |
denotes |
and |
T12166 |
645-647 |
, |
denotes |
, |
T12165 |
641-645 |
CD |
denotes |
0.05 |
T12164 |
640-641 |
SYM |
denotes |
< |
T12163 |
637-639 |
IN |
denotes |
of |
T12162 |
623-624 |
HYPH |
denotes |
- |
T12161 |
624-629 |
NN |
denotes |
value |
T12160 |
622-623 |
NN |
denotes |
p |
T12159 |
630-636 |
NN |
denotes |
cutoff |
T12158 |
620-621 |
DT |
denotes |
a |
T12157 |
615-619 |
IN |
denotes |
with |
T12156 |
613-615 |
, |
denotes |
, |
T12155 |
609-613 |
NNS |
denotes |
data |
T12154 |
605-608 |
DT |
denotes |
the |
T12153 |
602-604 |
IN |
denotes |
of |
T12152 |
584-594 |
NN |
denotes |
stringency |
T12151 |
580-583 |
JJ |
denotes |
low |
T12150 |
569-579 |
RB |
denotes |
relatively |
T12149 |
595-601 |
NN |
denotes |
screen |
T12148 |
567-568 |
DT |
denotes |
a |
T12147 |
561-566 |
VBG |
denotes |
using |
T12146 |
559-561 |
, |
denotes |
, |
T12145 |
552-559 |
VBN |
denotes |
changed |
T12144 |
538-551 |
RB |
denotes |
significantly |
T12143 |
535-537 |
IN |
denotes |
as |
T12142 |
519-523 |
VBD |
denotes |
were |
T12141 |
513-518 |
NNS |
denotes |
genes |
T12140 |
510-512 |
CD |
denotes |
45 |
T12139 |
508-510 |
, |
denotes |
, |
T12138 |
503-508 |
JJ |
denotes |
total |
T12137 |
524-534 |
VBN |
denotes |
identified |
T12136 |
500-502 |
IN |
denotes |
In |
T12135 |
499-676 |
sentence |
denotes |
In total, 45 genes were identified as significantly changed, using a relatively low stringency screen of the data, with a p-value cutoff of <0.05, and fold change >2 (Table 3). |
T12134 |
498-499 |
. |
denotes |
. |
T12133 |
488-498 |
NN |
denotes |
triplicate |
T12132 |
477-487 |
JJ |
denotes |
biological |
T12131 |
474-476 |
IN |
denotes |
in |
T12130 |
460-464 |
VBD |
denotes |
were |
T12129 |
465-473 |
VBN |
denotes |
examined |
T12128 |
450-451 |
SYM |
denotes |
- |
T12127 |
449-450 |
HYPH |
denotes |
/ |
T12126 |
448-449 |
SYM |
denotes |
- |
T12125 |
443-448 |
NN |
denotes |
Pygo2 |
T12124 |
442-443 |
HYPH |
denotes |
/ |
T12123 |
441-442 |
SYM |
denotes |
- |
T12122 |
440-441 |
HYPH |
denotes |
/ |
T12121 |
439-440 |
SYM |
denotes |
- |
T12120 |
434-439 |
NN |
denotes |
Pygo1 |
T12119 |
430-433 |
CC |
denotes |
and |
T12118 |
424-425 |
HYPH |
denotes |
- |
T12117 |
425-429 |
NN |
denotes |
type |
T12116 |
420-424 |
JJ |
denotes |
wild |
T12115 |
452-459 |
NNS |
denotes |
kidneys |
T12114 |
414-419 |
NN |
denotes |
E18.5 |
T12113 |
413-499 |
sentence |
denotes |
E18.5 wild-type and Pygo1-/-/Pygo2-/- kidneys were examined in biological triplicate. |
T12112 |
412-413 |
. |
denotes |
. |
T12111 |
401-404 |
NN |
denotes |
Wnt |
T12110 |
395-400 |
VBN |
denotes |
known |
T12109 |
405-412 |
NNS |
denotes |
targets |
T12108 |
391-394 |
DT |
denotes |
all |
T12107 |
381-390 |
VBG |
denotes |
including |
T12106 |
379-381 |
, |
denotes |
, |
T12105 |
374-379 |
NNS |
denotes |
genes |
T12104 |
370-373 |
DT |
denotes |
all |
T12103 |
367-369 |
IN |
denotes |
of |
T12102 |
359-366 |
NNS |
denotes |
changes |
T12101 |
348-358 |
NN |
denotes |
expression |
T12100 |
341-347 |
VB |
denotes |
follow |
T12099 |
338-340 |
TO |
denotes |
to |
T12098 |
330-332 |
VB |
denotes |
be |
T12097 |
326-329 |
MD |
denotes |
can |
T12096 |
314-325 |
NNS |
denotes |
microarrays |
T12095 |
312-314 |
, |
denotes |
, |
T12094 |
303-312 |
NN |
denotes |
signaling |
T12093 |
299-302 |
NN |
denotes |
Wnt |
T12092 |
296-298 |
IN |
denotes |
to |
T12091 |
287-295 |
NN |
denotes |
promoter |
T12090 |
283-286 |
CD |
denotes |
one |
T12089 |
280-282 |
IN |
denotes |
of |
T12088 |
271-279 |
NN |
denotes |
response |
T12087 |
267-270 |
DT |
denotes |
the |
T12086 |
333-337 |
VBN |
denotes |
used |
T12085 |
239-248 |
NN |
denotes |
transgene |
T12084 |
234-235 |
HYPH |
denotes |
- |
T12083 |
235-238 |
NN |
denotes |
gal |
T12082 |
231-234 |
NN |
denotes |
BAT |
T12081 |
249-257 |
NN |
denotes |
reporter |
T12080 |
227-230 |
DT |
denotes |
the |
T12079 |
258-266 |
VBZ |
denotes |
monitors |
T12078 |
219-226 |
IN |
denotes |
Whereas |
T12077 |
218-413 |
sentence |
denotes |
Whereas the BAT-gal transgene reporter monitors the response of one promoter to Wnt signaling, microarrays can be used to follow expression changes of all genes, including all known Wnt targets. |
T12076 |
217-218 |
. |
denotes |
. |
T12075 |
210-217 |
NNS |
denotes |
changes |
T12074 |
199-209 |
NN |
denotes |
expression |
T12073 |
194-198 |
NN |
denotes |
gene |
T12072 |
191-193 |
IN |
denotes |
of |
T12071 |
175-181 |
JJ |
denotes |
global |
T12070 |
182-190 |
NN |
denotes |
analysis |
T12069 |
173-174 |
DT |
denotes |
a |
T12068 |
165-172 |
VB |
denotes |
perform |
T12067 |
162-164 |
TO |
denotes |
to |
T12066 |
150-161 |
NNS |
denotes |
microarrays |
T12065 |
142-144 |
PRP |
denotes |
we |
T12064 |
140-142 |
, |
denotes |
, |
T12063 |
126-132 |
NN |
denotes |
mutant |
T12062 |
119-120 |
HYPH |
denotes |
/ |
T12061 |
120-125 |
NN |
denotes |
Pygo2 |
T12060 |
114-119 |
NN |
denotes |
Pygo1 |
T12059 |
133-140 |
NNS |
denotes |
kidneys |
T12058 |
110-113 |
DT |
denotes |
the |
T12057 |
107-109 |
IN |
denotes |
in |
T12056 |
96-106 |
NN |
denotes |
expression |
T12055 |
91-95 |
NN |
denotes |
gene |
T12054 |
88-90 |
IN |
denotes |
of |
T12053 |
76-87 |
NN |
denotes |
disturbance |
T12052 |
67-75 |
JJ |
denotes |
possible |
T12051 |
145-149 |
VBD |
denotes |
used |
T12050 |
51-58 |
RB |
denotes |
further |
T12049 |
59-66 |
VB |
denotes |
examine |
T12048 |
48-50 |
TO |
denotes |
To |
T12047 |
47-218 |
sentence |
denotes |
To further examine possible disturbance of gene expression in the Pygo1/Pygo2 mutant kidneys, we used microarrays to perform a global analysis of gene expression changes. |
T12046 |
40-47 |
NNS |
denotes |
kidneys |
T12045 |
35-39 |
JJ |
denotes |
null |
T12044 |
28-29 |
HYPH |
denotes |
/ |
T12043 |
29-34 |
NN |
denotes |
Pygo2 |
T12042 |
23-28 |
NN |
denotes |
Pygo1 |
T12041 |
20-22 |
IN |
denotes |
of |
T12040 |
11-19 |
NN |
denotes |
analysis |
T12039 |
0-10 |
NN |
denotes |
Microarray |
T12182 |
691-696 |
NN |
denotes |
Pygo1 |
T12181 |
686-690 |
CC |
denotes |
both |
T12180 |
684-686 |
, |
denotes |
, |
T12179 |
712-722 |
VBN |
denotes |
identified |
T12178 |
677-684 |
RB |
denotes |
Notably |
T12177 |
676-804 |
sentence |
denotes |
Notably, both Pygo1 and Pygo2 were identified as downregulated (- 5.3-fold and - 3.9-fold, respectively) in the mutant kidneys. |
T12176 |
675-676 |
. |
denotes |
. |
T12175 |
674-675 |
-RRB- |
denotes |
) |
T12174 |
673-674 |
CD |
denotes |
3 |
T12173 |
667-672 |
NN |
denotes |
Table |
T12172 |
666-667 |
-LRB- |
denotes |
( |
T12171 |
664-665 |
CD |
denotes |
2 |
T12170 |
663-664 |
SYM |
denotes |
> |
T12169 |
656-662 |
NN |
denotes |
change |
T12168 |
651-655 |
NN |
denotes |
fold |
T12215 |
879-880 |
SYM |
denotes |
- |
T27315 |
5136-5140 |
IN |
denotes |
from |
T27314 |
5122-5126 |
VBD |
denotes |
were |
T27313 |
5127-5135 |
VBN |
denotes |
selected |
T27312 |
5110-5113 |
NN |
denotes |
Wnt |
T27311 |
5114-5121 |
NNS |
denotes |
targets |
T27377 |
5459-5460 |
. |
denotes |
. |
T27376 |
5453-5459 |
NNS |
denotes |
levels |
T27375 |
5442-5452 |
NN |
denotes |
expression |
T27374 |
5439-5441 |
IN |
denotes |
in |
T27373 |
5431-5438 |
NNS |
denotes |
changes |
T27372 |
5419-5430 |
JJ |
denotes |
significant |
T27661 |
5921-5924 |
DT |
denotes |
the |
T27660 |
5918-5920 |
IN |
denotes |
in |
T27659 |
5910-5917 |
NNS |
denotes |
changes |
T27658 |
5899-5909 |
NN |
denotes |
expression |
T27657 |
5894-5898 |
NN |
denotes |
gene |
T27656 |
5891-5893 |
IN |
denotes |
of |
T27655 |
5880-5890 |
NN |
denotes |
Validation |
T27599 |
1499-1500 |
. |
denotes |
. |
T27598 |
1492-1499 |
NNS |
denotes |
samples |
T27597 |
1486-1487 |
HYPH |
denotes |
- |
T27596 |
1487-1491 |
NN |
denotes |
type |
T27595 |
1482-1486 |
JJ |
denotes |
wild |
T27594 |
1479-1481 |
IN |
denotes |
to |
T27593 |
1468-1478 |
VBN |
denotes |
normalized |
T27592 |
1455-1460 |
NN |
denotes |
E18.5 |
T27591 |
1450-1454 |
JJ |
denotes |
null |
T27590 |
1443-1444 |
HYPH |
denotes |
/ |
T27675 |
5993-5994 |
. |
denotes |
. |
T27674 |
5980-5981 |
HYPH |
denotes |
- |
T27673 |
5981-5985 |
NN |
denotes |
type |
T27672 |
5976-5980 |
JJ |
denotes |
wild |
T27671 |
5986-5993 |
NNS |
denotes |
kidneys |
T27670 |
5970-5975 |
NN |
denotes |
E18.5 |
T27669 |
5967-5969 |
IN |
denotes |
to |
T27668 |
5956-5966 |
VBN |
denotes |
normalized |
T27667 |
5943-5947 |
JJ |
denotes |
null |
T27666 |
5936-5937 |
HYPH |
denotes |
/ |
T27665 |
5937-5942 |
NN |
denotes |
Pygo2 |
T27664 |
5931-5936 |
NN |
denotes |
Pygo1 |
T27663 |
5925-5930 |
NN |
denotes |
E18.5 |
T27662 |
5948-5955 |
NNS |
denotes |
kidneys |
T12301 |
1298-1299 |
DT |
denotes |
a |
T12300 |
1287-1292 |
MD |
denotes |
might |
T12299 |
1274-1275 |
HYPH |
denotes |
/ |
T12298 |
1275-1280 |
NN |
denotes |
Pygo2 |
T12297 |
1269-1274 |
NN |
denotes |
Pygo1 |
T12296 |
1281-1286 |
NNS |
denotes |
genes |
T12295 |
1265-1268 |
DT |
denotes |
the |
T12294 |
1293-1297 |
VB |
denotes |
play |
T12293 |
1260-1264 |
IN |
denotes |
that |
T12292 |
1252-1259 |
VBP |
denotes |
suggest |
T12291 |
1244-1251 |
NNS |
denotes |
results |
T12290 |
1238-1243 |
DT |
denotes |
These |
T12289 |
1237-1351 |
sentence |
denotes |
These results suggest that the Pygo1/Pygo2 genes might play a role in nephron maturation and subsequent function. |
T12288 |
1236-1237 |
. |
denotes |
. |
T12596 |
4813-4815 |
IN |
denotes |
of |
T12595 |
4802-4812 |
NN |
denotes |
expression |
T12594 |
4799-4801 |
IN |
denotes |
in |
T12593 |
4791-4798 |
NNS |
denotes |
changes |
T12592 |
4782-4790 |
JJ |
denotes |
dramatic |
T12591 |
4779-4781 |
IN |
denotes |
of |
T12590 |
4771-4778 |
NN |
denotes |
absence |
T12589 |
4768-4770 |
DT |
denotes |
an |
T12588 |
4759-4767 |
VBP |
denotes |
indicate |
T12587 |
4751-4758 |
NNS |
denotes |
results |
T12586 |
4745-4750 |
DT |
denotes |
These |
T12585 |
4744-4894 |
sentence |
denotes |
These results indicate an absence of dramatic changes in expression of previously known Wnt signaling target genes in the Pygo1/Pygo2 mutant kidneys. |
T12584 |
4743-4744 |
. |
denotes |
. |
T12583 |
4736-4743 |
NNS |
denotes |
kidneys |
T12582 |
4731-4735 |
JJ |
denotes |
null |
T12581 |
4724-4725 |
HYPH |
denotes |
/ |
T12580 |
4725-4730 |
NN |
denotes |
Pygo2 |
T12579 |
4719-4724 |
NN |
denotes |
Pygo1 |
T12578 |
4716-4718 |
IN |
denotes |
in |
T12577 |
4707-4715 |
RB |
denotes |
1.5-fold |
T12576 |
4706-4707 |
SYM |
denotes |
< |
T12575 |
4703-4705 |
IN |
denotes |
of |
T12574 |
4695-4702 |
NNS |
denotes |
changes |
T12573 |
4689-4694 |
NN |
denotes |
level |
T12572 |
4678-4688 |
NN |
denotes |
expression |
T12614 |
5469-5481 |
JJ |
denotes |
quantitative |
T12613 |
5464-5468 |
VBD |
denotes |
used |
T12612 |
5461-5463 |
PRP |
denotes |
We |
T12611 |
4894-5569 |
sentence |
denotes |
Figure 8 Gene expression changes of common Wnt signaling targets in the E18.5 Pygo1/Pygo2 null kidney. Microarray analysis was performed in triplicate on wild-type and Pygo1/2 compound null E18.5 kidneys. Possible Wnt targets were selected from those compiled at [39]. An initial gene list of 82 Wnt targets was created and then reduced to a total of 40 genes using an expression level restriction requiring the raw expression intensity to be >100 in at least 3 samples. Pygo1 and Pygo2 probes were included to demonstrate significant changes in expression levels. We used quantitative real-time PCR, with independent biological samples, to validate the microarray results. |
T12651 |
5671-5680 |
VBN |
denotes |
predicted |
T12650 |
5656-5660 |
JJ |
denotes |
same |
T12316 |
3401-3403 |
IN |
denotes |
in |
T12315 |
3389-3400 |
VBN |
denotes |
upregulated |
T12314 |
3432-3440 |
VBN |
denotes |
included |
T12313 |
3383-3388 |
NNS |
denotes |
genes |
T12312 |
3372-3382 |
JJ |
denotes |
Noteworthy |
T12311 |
3372-3516 |
sentence |
denotes |
Noteworthy genes upregulated in the Pygo1/Pygo2 null kidney included Klk5 (2.8-fold), Klk6 (2.7-fold), Ren2 (2.2-fold), and Timeless (2.4-fold). |
T12310 |
1351-1352 |
sentence |
denotes |
|
T12309 |
1350-1351 |
. |
denotes |
. |
T12308 |
1342-1350 |
NN |
denotes |
function |
T12307 |
1331-1341 |
JJ |
denotes |
subsequent |
T12306 |
1327-1330 |
CC |
denotes |
and |
T12305 |
1316-1326 |
NN |
denotes |
maturation |
T12304 |
1308-1315 |
NN |
denotes |
nephron |
T12303 |
1305-1307 |
IN |
denotes |
in |
T12302 |
1300-1304 |
NN |
denotes |
role |
T12362 |
3602-3613 |
NNS |
denotes |
Kallikreins |
T12610 |
4893-4894 |
. |
denotes |
. |
T12609 |
4879-4885 |
NN |
denotes |
mutant |
T12608 |
4872-4873 |
HYPH |
denotes |
/ |
T12607 |
4873-4878 |
NN |
denotes |
Pygo2 |
T12606 |
4867-4872 |
NN |
denotes |
Pygo1 |
T12605 |
4886-4893 |
NNS |
denotes |
kidneys |
T12604 |
4863-4866 |
DT |
denotes |
the |
T12603 |
4860-4862 |
IN |
denotes |
in |
T12602 |
4847-4853 |
NN |
denotes |
target |
T12601 |
4837-4846 |
NN |
denotes |
signaling |
T12600 |
4833-4836 |
NN |
denotes |
Wnt |
T12599 |
4854-4859 |
NNS |
denotes |
genes |
T12598 |
4827-4832 |
VBN |
denotes |
known |
T12597 |
4816-4826 |
RB |
denotes |
previously |
T12649 |
5661-5670 |
NN |
denotes |
direction |
T12648 |
5652-5655 |
DT |
denotes |
the |
T12647 |
5649-5651 |
IN |
denotes |
in |
T12646 |
5637-5641 |
NN |
denotes |
fold |
T12645 |
5625-5636 |
JJ |
denotes |
significant |
T12644 |
5642-5648 |
NN |
denotes |
change |
T12643 |
5623-5624 |
DT |
denotes |
a |
T12642 |
5611-5614 |
VBD |
denotes |
did |
T12641 |
5608-5610 |
PRP |
denotes |
we |
T12640 |
5602-5607 |
CD |
denotes |
seven |
T12639 |
5615-5622 |
VB |
denotes |
observe |
T12638 |
5598-5601 |
IN |
denotes |
for |
T12637 |
5594-5597 |
CC |
denotes |
and |
T12636 |
5592-5594 |
, |
denotes |
, |
T12635 |
5581-5585 |
VBD |
denotes |
were |
T12634 |
5586-5592 |
VBN |
denotes |
tested |
T12633 |
5575-5580 |
NNS |
denotes |
genes |
T12632 |
5570-5574 |
CD |
denotes |
Nine |
T12631 |
5569-5717 |
sentence |
denotes |
Nine genes were tested, and for seven we did observe a significant fold change in the same direction predicted by the microarray results (Table 4). |
T12630 |
5568-5569 |
. |
denotes |
. |
T12629 |
5550-5560 |
NN |
denotes |
microarray |
T12628 |
5561-5568 |
NNS |
denotes |
results |
T12627 |
5546-5549 |
DT |
denotes |
the |
T12626 |
5537-5545 |
VB |
denotes |
validate |
T12625 |
5534-5536 |
TO |
denotes |
to |
T12624 |
5532-5534 |
, |
denotes |
, |
T12623 |
5514-5524 |
JJ |
denotes |
biological |
T12622 |
5525-5532 |
NNS |
denotes |
samples |
T12621 |
5502-5513 |
JJ |
denotes |
independent |
T12620 |
5497-5501 |
IN |
denotes |
with |
T12619 |
5495-5497 |
, |
denotes |
, |
T12618 |
5486-5487 |
HYPH |
denotes |
- |
T12617 |
5487-5491 |
NN |
denotes |
time |
T12616 |
5482-5486 |
JJ |
denotes |
real |
T12615 |
5492-5495 |
NN |
denotes |
PCR |
R3017 |
T12039 |
T12040 |
compound |
Microarray,analysis |
R3018 |
T12041 |
T12040 |
prep |
of,analysis |
R3019 |
T12042 |
T12043 |
compound |
Pygo1,Pygo2 |
R3020 |
T12043 |
T12045 |
npadvmod |
Pygo2,null |
R3021 |
T12044 |
T12043 |
punct |
/,Pygo2 |
R3022 |
T12045 |
T12046 |
amod |
null,kidneys |
R3023 |
T12046 |
T12041 |
pobj |
kidneys,of |
R3024 |
T12048 |
T12049 |
aux |
To,examine |
R3025 |
T12049 |
T12051 |
advcl |
examine,used |
R3026 |
T12050 |
T12049 |
advmod |
further,examine |
R3027 |
T12052 |
T12053 |
amod |
possible,disturbance |
R3028 |
T12053 |
T12049 |
dobj |
disturbance,examine |
R3029 |
T12054 |
T12053 |
prep |
of,disturbance |
R3030 |
T12055 |
T12056 |
compound |
gene,expression |
R3031 |
T12056 |
T12054 |
pobj |
expression,of |
R3032 |
T12057 |
T12049 |
prep |
in,examine |
R3033 |
T12058 |
T12059 |
det |
the,kidneys |
R3034 |
T12059 |
T12057 |
pobj |
kidneys,in |
R3035 |
T12060 |
T12061 |
compound |
Pygo1,Pygo2 |
R3036 |
T12061 |
T12059 |
compound |
Pygo2,kidneys |
R3037 |
T12062 |
T12061 |
punct |
/,Pygo2 |
R3038 |
T12063 |
T12059 |
compound |
mutant,kidneys |
R3039 |
T12064 |
T12051 |
punct |
", ",used |
R3040 |
T12065 |
T12051 |
nsubj |
we,used |
R3041 |
T12066 |
T12051 |
dobj |
microarrays,used |
R3042 |
T12067 |
T12068 |
aux |
to,perform |
R3043 |
T12068 |
T12051 |
advcl |
perform,used |
R3044 |
T12069 |
T12070 |
det |
a,analysis |
R3045 |
T12070 |
T12068 |
dobj |
analysis,perform |
R3046 |
T12071 |
T12070 |
amod |
global,analysis |
R3047 |
T12072 |
T12070 |
prep |
of,analysis |
R3048 |
T12073 |
T12074 |
compound |
gene,expression |
R3049 |
T12074 |
T12075 |
compound |
expression,changes |
R3050 |
T12075 |
T12072 |
pobj |
changes,of |
R3051 |
T12076 |
T12051 |
punct |
.,used |
R3052 |
T12078 |
T12079 |
mark |
Whereas,monitors |
R3053 |
T12079 |
T12086 |
advcl |
monitors,used |
R3054 |
T12080 |
T12081 |
det |
the,reporter |
R3055 |
T12081 |
T12079 |
nsubj |
reporter,monitors |
R3056 |
T12082 |
T12083 |
compound |
BAT,gal |
R3057 |
T12083 |
T12085 |
compound |
gal,transgene |
R3058 |
T12084 |
T12083 |
punct |
-,gal |
R3059 |
T12085 |
T12081 |
compound |
transgene,reporter |
R3060 |
T12087 |
T12088 |
det |
the,response |
R3061 |
T12088 |
T12079 |
dobj |
response,monitors |
R3062 |
T12089 |
T12088 |
prep |
of,response |
R3063 |
T12090 |
T12091 |
nummod |
one,promoter |
R3064 |
T12091 |
T12089 |
pobj |
promoter,of |
R3065 |
T12092 |
T12088 |
prep |
to,response |
R3066 |
T12093 |
T12094 |
compound |
Wnt,signaling |
R3067 |
T12094 |
T12092 |
pobj |
signaling,to |
R3068 |
T12095 |
T12086 |
punct |
", ",used |
R3069 |
T12096 |
T12086 |
nsubjpass |
microarrays,used |
R3070 |
T12097 |
T12086 |
aux |
can,used |
R3071 |
T12098 |
T12086 |
auxpass |
be,used |
R3072 |
T12099 |
T12100 |
aux |
to,follow |
R3073 |
T12100 |
T12086 |
advcl |
follow,used |
R3074 |
T12101 |
T12102 |
compound |
expression,changes |
R3075 |
T12102 |
T12100 |
dobj |
changes,follow |
R3076 |
T12103 |
T12102 |
prep |
of,changes |
R3077 |
T12104 |
T12105 |
det |
all,genes |
R3078 |
T12105 |
T12103 |
pobj |
genes,of |
R3079 |
T12106 |
T12105 |
punct |
", ",genes |
R3080 |
T12107 |
T12105 |
prep |
including,genes |
R3081 |
T12108 |
T12109 |
det |
all,targets |
R3082 |
T12109 |
T12107 |
pobj |
targets,including |
R3083 |
T12110 |
T12109 |
amod |
known,targets |
R3084 |
T12111 |
T12109 |
compound |
Wnt,targets |
R3085 |
T12112 |
T12086 |
punct |
.,used |
R3086 |
T12114 |
T12115 |
nmod |
E18.5,kidneys |
R3087 |
T12115 |
T12129 |
nsubjpass |
kidneys,examined |
R3088 |
T12116 |
T12117 |
amod |
wild,type |
R3089 |
T12117 |
T12115 |
nmod |
type,kidneys |
R3090 |
T12118 |
T12117 |
punct |
-,type |
R3091 |
T12119 |
T12117 |
cc |
and,type |
R3092 |
T12120 |
T12117 |
conj |
Pygo1,type |
R3093 |
T12121 |
T12120 |
punct |
-,Pygo1 |
R3094 |
T12122 |
T12120 |
punct |
/,Pygo1 |
R3095 |
T12123 |
T12120 |
punct |
-,Pygo1 |
R3096 |
T12124 |
T12120 |
punct |
/,Pygo1 |
R3097 |
T12125 |
T12120 |
appos |
Pygo2,Pygo1 |
R3098 |
T12126 |
T12125 |
punct |
-,Pygo2 |
R3099 |
T12127 |
T12125 |
punct |
/,Pygo2 |
R3100 |
T12128 |
T12125 |
punct |
-,Pygo2 |
R3101 |
T12130 |
T12129 |
auxpass |
were,examined |
R3102 |
T12131 |
T12129 |
prep |
in,examined |
R3103 |
T12132 |
T12133 |
amod |
biological,triplicate |
R3104 |
T12133 |
T12131 |
pobj |
triplicate,in |
R3105 |
T12134 |
T12129 |
punct |
.,examined |
R3106 |
T12136 |
T12137 |
prep |
In,identified |
R3107 |
T12138 |
T12136 |
amod |
total,In |
R3108 |
T12139 |
T12137 |
punct |
", ",identified |
R3109 |
T12140 |
T12141 |
nummod |
45,genes |
R3110 |
T12141 |
T12137 |
nsubjpass |
genes,identified |
R3111 |
T12142 |
T12137 |
auxpass |
were,identified |
R3112 |
T12143 |
T12137 |
prep |
as,identified |
R3113 |
T12144 |
T12145 |
advmod |
significantly,changed |
R3114 |
T12145 |
T12143 |
amod |
changed,as |
R3115 |
T12146 |
T12137 |
punct |
", ",identified |
R3116 |
T12147 |
T12137 |
advcl |
using,identified |
R3117 |
T12148 |
T12149 |
det |
a,screen |
R3118 |
T12149 |
T12147 |
dobj |
screen,using |
R3119 |
T12150 |
T12151 |
advmod |
relatively,low |
R3120 |
T12151 |
T12149 |
amod |
low,screen |
R3121 |
T12152 |
T12149 |
compound |
stringency,screen |
R3122 |
T12153 |
T12149 |
prep |
of,screen |
R3123 |
T12154 |
T12155 |
det |
the,data |
R3124 |
T12155 |
T12153 |
pobj |
data,of |
R3125 |
T12156 |
T12137 |
punct |
", ",identified |
R3126 |
T12157 |
T12137 |
prep |
with,identified |
R3127 |
T12158 |
T12159 |
det |
a,cutoff |
R3128 |
T12159 |
T12157 |
pobj |
cutoff,with |
R3129 |
T12160 |
T12161 |
compound |
p,value |
R3130 |
T12161 |
T12159 |
compound |
value,cutoff |
R3131 |
T12162 |
T12161 |
punct |
-,value |
R3132 |
T12163 |
T12159 |
prep |
of,cutoff |
R3133 |
T12164 |
T12165 |
punct |
<,0.05 |
R3134 |
T12165 |
T12163 |
pobj |
0.05,of |
R3135 |
T12166 |
T12159 |
punct |
", ",cutoff |
R3136 |
T12167 |
T12159 |
cc |
and,cutoff |
R3137 |
T12168 |
T12169 |
compound |
fold,change |
R3138 |
T12169 |
T12159 |
conj |
change,cutoff |
R3139 |
T12170 |
T12171 |
punct |
>,2 |
R3140 |
T12171 |
T12169 |
appos |
2,change |
R3141 |
T12172 |
T12173 |
punct |
(,Table |
R3142 |
T12173 |
T12137 |
parataxis |
Table,identified |
R3143 |
T12174 |
T12173 |
nummod |
3,Table |
R3144 |
T12175 |
T12173 |
punct |
),Table |
R3145 |
T12176 |
T12137 |
punct |
.,identified |
R3146 |
T12178 |
T12179 |
advmod |
Notably,identified |
R3147 |
T12180 |
T12179 |
punct |
", ",identified |
R3148 |
T12181 |
T12182 |
preconj |
both,Pygo1 |
R3149 |
T12182 |
T12179 |
nsubjpass |
Pygo1,identified |
R3150 |
T12183 |
T12182 |
cc |
and,Pygo1 |
R3151 |
T12184 |
T12182 |
conj |
Pygo2,Pygo1 |
R3152 |
T12185 |
T12179 |
auxpass |
were,identified |
R3153 |
T12186 |
T12179 |
prep |
as,identified |
R3154 |
T12187 |
T12186 |
amod |
downregulated,as |
R3155 |
T12188 |
T12189 |
punct |
(,respectively |
R3156 |
T12189 |
T12187 |
parataxis |
respectively,downregulated |
R3157 |
T12190 |
T12191 |
punct |
-,5.3-fold |
R3158 |
T12191 |
T12189 |
advmod |
5.3-fold,respectively |
R3159 |
T12192 |
T12191 |
cc |
and,5.3-fold |
R3160 |
T12193 |
T12194 |
punct |
-,3.9-fold |
R3161 |
T12194 |
T12191 |
conj |
3.9-fold,5.3-fold |
R3162 |
T12195 |
T12189 |
punct |
", ",respectively |
R3163 |
T12196 |
T12189 |
punct |
),respectively |
R3164 |
T12197 |
T12179 |
prep |
in,identified |
R3165 |
T12198 |
T12199 |
det |
the,kidneys |
R3166 |
T12199 |
T12197 |
pobj |
kidneys,in |
R3167 |
T12200 |
T12199 |
compound |
mutant,kidneys |
R3168 |
T12201 |
T12179 |
punct |
.,identified |
R3169 |
T12203 |
T12204 |
amod |
Other,genes |
R3170 |
T12204 |
T12205 |
nsubj |
genes,included |
R3171 |
T12206 |
T12204 |
prep |
with,genes |
R3172 |
T12207 |
T12208 |
amod |
significant,decreases |
R3173 |
T12208 |
T12206 |
pobj |
decreases,with |
R3174 |
T12209 |
T12208 |
prep |
in,decreases |
R3175 |
T12210 |
T12211 |
compound |
mutant,kidneys |
R3176 |
T12211 |
T12209 |
pobj |
kidneys,in |
R3177 |
T12212 |
T12205 |
dobj |
Cxcl13,included |
R3178 |
T12213 |
T12214 |
punct |
(,2.3-fold |
R3179 |
T12214 |
T12212 |
parataxis |
2.3-fold,Cxcl13 |
R3180 |
T12215 |
T12214 |
punct |
-,2.3-fold |
R3181 |
T12216 |
T12214 |
punct |
),2.3-fold |
R3182 |
T12217 |
T12212 |
punct |
", ",Cxcl13 |
R3183 |
T12218 |
T12212 |
conj |
Slc5a2,Cxcl13 |
R3184 |
T12219 |
T12220 |
punct |
(,2.8-fold |
R3185 |
T12220 |
T12218 |
parataxis |
2.8-fold,Slc5a2 |
R3186 |
T12221 |
T12220 |
punct |
-,2.8-fold |
R3187 |
T12222 |
T12220 |
punct |
),2.8-fold |
R3188 |
T12223 |
T12218 |
punct |
", ",Slc5a2 |
R3189 |
T12224 |
T12218 |
cc |
and,Slc5a2 |
R3190 |
T12225 |
T12218 |
conj |
Slco1a4,Slc5a2 |
R3191 |
T12226 |
T12227 |
punct |
(,2.1-fold |
R3192 |
T12227 |
T12225 |
parataxis |
2.1-fold,Slco1a4 |
R3193 |
T12228 |
T12227 |
punct |
-,2.1-fold |
R3194 |
T12229 |
T12227 |
punct |
),2.1-fold |
R3195 |
T12230 |
T12205 |
punct |
.,included |
R3196 |
T12232 |
T12233 |
nsubjpass |
Slc5a2,expressed |
R3197 |
T12234 |
T12233 |
auxpass |
is,expressed |
R3198 |
T12235 |
T12233 |
prep |
in,expressed |
R3199 |
T12236 |
T12237 |
det |
the,tubules |
R3200 |
T12237 |
T12235 |
pobj |
tubules,in |
R3201 |
T12238 |
T12237 |
amod |
proximal,tubules |
R3202 |
T12239 |
T12237 |
prep |
of,tubules |
R3203 |
T12240 |
T12241 |
det |
the,nephron |
R3204 |
T12241 |
T12239 |
pobj |
nephron,of |
R3205 |
T12242 |
T12241 |
amod |
adult,nephron |
R3206 |
T12243 |
T12233 |
cc |
and,expressed |
R3207 |
T12244 |
T12245 |
aux |
has,implicated |
R3208 |
T12245 |
T12233 |
conj |
implicated,expressed |
R3209 |
T12246 |
T12245 |
auxpass |
been,implicated |
R3210 |
T12247 |
T12245 |
prep |
in,implicated |
R3211 |
T12248 |
T12249 |
amod |
autosomal,recessive |
R3212 |
T12249 |
T12250 |
amod |
recessive,glucosuria |
R3213 |
T12250 |
T12247 |
pobj |
glucosuria,in |
R3214 |
T12251 |
T12250 |
amod |
renal,glucosuria |
R3215 |
T12252 |
T12250 |
punct |
", ",glucosuria |
R3216 |
T12253 |
T12250 |
acl |
characterized,glucosuria |
R3217 |
T12254 |
T12253 |
agent |
by,characterized |
R3218 |
T12255 |
T12254 |
pobj |
loss,by |
R3219 |
T12256 |
T12255 |
prep |
of,loss |
R3220 |
T12257 |
T12258 |
compound |
glucose,uptake |
R3221 |
T12258 |
T12256 |
pobj |
uptake,of |
R3222 |
T12259 |
T12258 |
prep |
by,uptake |
R3223 |
T12260 |
T12261 |
det |
the,nephron |
R3224 |
T12261 |
T12259 |
pobj |
nephron,by |
R3225 |
T12262 |
T12263 |
punct |
[,23 |
R3226 |
T12263 |
T12253 |
parataxis |
23,characterized |
R3227 |
T12264 |
T12263 |
punct |
],23 |
R3228 |
T12265 |
T12233 |
punct |
.,expressed |
R3229 |
T12267 |
T12268 |
det |
The,transporter |
R3230 |
T12268 |
T12271 |
nsubjpass |
transporter,expressed |
R3231 |
T12269 |
T12268 |
amod |
organic,transporter |
R3232 |
T12270 |
T12268 |
compound |
anion,transporter |
R3233 |
T12272 |
T12268 |
punct |
", ",transporter |
R3234 |
T12273 |
T12268 |
appos |
Slco1a4,transporter |
R3235 |
T12274 |
T12271 |
punct |
", ",expressed |
R3236 |
T12275 |
T12271 |
auxpass |
is,expressed |
R3237 |
T12276 |
T12271 |
advmod |
also,expressed |
R3266 |
T12307 |
T12308 |
amod |
subsequent,function |
R3267 |
T12308 |
T12305 |
conj |
function,maturation |
R3268 |
T12309 |
T12292 |
punct |
.,suggest |
R3269 |
T12312 |
T12313 |
amod |
Noteworthy,genes |
R3270 |
T12313 |
T12314 |
nsubj |
genes,included |
R3271 |
T12315 |
T12313 |
acl |
upregulated,genes |
R3272 |
T12316 |
T12315 |
prep |
in,upregulated |
R3273 |
T12317 |
T12318 |
det |
the,kidney |
R3274 |
T12318 |
T12316 |
pobj |
kidney,in |
R3275 |
T12319 |
T12320 |
compound |
Pygo1,Pygo2 |
R3276 |
T12320 |
T12322 |
npadvmod |
Pygo2,null |
R3277 |
T12321 |
T12320 |
punct |
/,Pygo2 |
R3278 |
T12322 |
T12318 |
amod |
null,kidney |
R3279 |
T12323 |
T12314 |
dobj |
Klk5,included |
R3280 |
T12324 |
T12325 |
punct |
(,2.8-fold |
R3281 |
T12325 |
T12323 |
parataxis |
2.8-fold,Klk5 |
R3282 |
T12326 |
T12325 |
punct |
),2.8-fold |
R3283 |
T12327 |
T12323 |
punct |
", ",Klk5 |
R3284 |
T12328 |
T12323 |
conj |
Klk6,Klk5 |
R3285 |
T12329 |
T12330 |
punct |
(,2.7-fold |
R3286 |
T12330 |
T12328 |
parataxis |
2.7-fold,Klk6 |
R3287 |
T12331 |
T12330 |
punct |
),2.7-fold |
R3288 |
T12332 |
T12328 |
punct |
", ",Klk6 |
R3289 |
T12333 |
T12328 |
conj |
Ren2,Klk6 |
R3290 |
T12334 |
T12335 |
punct |
(,2.2-fold |
R3291 |
T12335 |
T12333 |
parataxis |
2.2-fold,Ren2 |
R3292 |
T12336 |
T12335 |
punct |
),2.2-fold |
R3293 |
T12337 |
T12333 |
punct |
", ",Ren2 |
R3294 |
T12338 |
T12333 |
cc |
and,Ren2 |
R3295 |
T12339 |
T12333 |
conj |
Timeless,Ren2 |
R3296 |
T12340 |
T12341 |
punct |
(,2.4-fold |
R3297 |
T12341 |
T12339 |
parataxis |
2.4-fold,Timeless |
R3298 |
T12342 |
T12341 |
punct |
),2.4-fold |
R3299 |
T12343 |
T12314 |
punct |
.,included |
R3300 |
T12345 |
T12346 |
nsubj |
Klk5,are |
R3301 |
T12347 |
T12345 |
cc |
and,Klk5 |
R3302 |
T12348 |
T12345 |
conj |
Klk6,Klk5 |
R3303 |
T12349 |
T12346 |
attr |
members,are |
R3304 |
T12350 |
T12349 |
prep |
of,members |
R3305 |
T12351 |
T12352 |
det |
the,family |
R3306 |
T12352 |
T12350 |
pobj |
family,of |
R3307 |
T12353 |
T12352 |
compound |
kallikrein,family |
R3308 |
T12354 |
T12352 |
prep |
of,family |
R3309 |
T12355 |
T12356 |
npadvmod |
trypsin,like |
R3310 |
T12356 |
T12358 |
amod |
like,proteases |
R3311 |
T12357 |
T12356 |
punct |
-,like |
R3312 |
T12358 |
T12354 |
pobj |
proteases,of |
R3313 |
T12359 |
T12358 |
compound |
serine,proteases |
R3314 |
T12360 |
T12346 |
punct |
.,are |
R3315 |
T12362 |
T12363 |
nsubj |
Kallikreins,have |
R3316 |
T12364 |
T12365 |
amod |
diverse,functions |
R3317 |
T12365 |
T12363 |
dobj |
functions,have |
R3318 |
T12366 |
T12363 |
prep |
in,have |
R3319 |
T12367 |
T12366 |
pobj |
cancer,in |
R3320 |
T12368 |
T12367 |
punct |
", ",cancer |
R3321 |
T12369 |
T12370 |
compound |
tissue,remodeling |
R3322 |
T12370 |
T12367 |
conj |
remodeling,cancer |
R3323 |
T12371 |
T12370 |
punct |
", ",remodeling |
R3324 |
T12372 |
T12370 |
cc |
and,remodeling |
R3325 |
T12373 |
T12370 |
conj |
regulation,remodeling |
R3326 |
T12374 |
T12373 |
prep |
of,regulation |
R3327 |
T12375 |
T12376 |
compound |
blood,pressure |
R3328 |
T12376 |
T12374 |
pobj |
pressure,of |
R3329 |
T12377 |
T12378 |
punct |
[,25 |
R3330 |
T12378 |
T12363 |
parataxis |
25,have |
R3331 |
T12379 |
T12378 |
punct |
],25 |
R3332 |
T12380 |
T12363 |
punct |
.,have |
R3333 |
T12382 |
T12383 |
nsubj |
Ren2,activates |
R3334 |
T12384 |
T12382 |
punct |
", ",Ren2 |
R3335 |
T12385 |
T12386 |
det |
a,homolog |
R3336 |
T12386 |
T12382 |
appos |
homolog,Ren2 |
R3337 |
T12387 |
T12386 |
prep |
of,homolog |
R3338 |
T12388 |
T12389 |
det |
the,endopeptidase |
R3339 |
T12389 |
T12387 |
pobj |
endopeptidase,of |
R3340 |
T12390 |
T12389 |
appos |
Renin,endopeptidase |
R3341 |
T12391 |
T12390 |
nummod |
1,Renin |
R3342 |
T12392 |
T12383 |
punct |
", ",activates |
R3343 |
T12393 |
T12394 |
det |
the,system |
R3344 |
T12394 |
T12383 |
dobj |
system,activates |
R3345 |
T12395 |
T12396 |
compound |
renin,angiotensin |
R3346 |
T12396 |
T12394 |
compound |
angiotensin,system |
R3347 |
T12397 |
T12396 |
punct |
-,angiotensin |
R3348 |
T12398 |
T12383 |
punct |
", ",activates |
R3349 |
T12399 |
T12383 |
advcl |
increasing,activates |
R3350 |
T12400 |
T12401 |
compound |
blood,pressure |
R3351 |
T12401 |
T12399 |
dobj |
pressure,increasing |
R3352 |
T12402 |
T12403 |
punct |
[,26 |
R3353 |
T12403 |
T12383 |
parataxis |
26,activates |
R3354 |
T12404 |
T12403 |
punct |
],26 |
R3355 |
T12405 |
T12383 |
punct |
.,activates |
R3356 |
T12407 |
T12408 |
nsubj |
Disruption,results |
R3357 |
T12409 |
T12407 |
prep |
of,Disruption |
R3358 |
T12410 |
T12411 |
det |
the,system |
R3359 |
T12411 |
T12409 |
pobj |
system,of |
R3360 |
T12412 |
T12413 |
compound |
renin,angiotensin |
R3361 |
T12413 |
T12411 |
compound |
angiotensin,system |
R3362 |
T12414 |
T12413 |
punct |
-,angiotensin |
R3363 |
T12415 |
T12407 |
prep |
during,Disruption |
R3364 |
T12416 |
T12415 |
pobj |
development,during |
R3365 |
T12417 |
T12408 |
prep |
in,results |
R3366 |
T12418 |
T12419 |
amod |
congenital,abnormalities |
R3367 |
T12419 |
T12417 |
pobj |
abnormalities,in |
R3368 |
T12420 |
T12419 |
prep |
of,abnormalities |
R3369 |
T12421 |
T12422 |
det |
the,ureter |
R3370 |
T12422 |
T12420 |
pobj |
ureter,of |
R3371 |
T12423 |
T12422 |
cc |
and,ureter |
R3372 |
T12424 |
T12425 |
compound |
collecting,duct |
R3373 |
T12425 |
T12427 |
compound |
duct,system |
R3374 |
T12426 |
T12425 |
punct |
-,duct |
R3375 |
T12427 |
T12422 |
conj |
system,ureter |
R3376 |
T12428 |
T12429 |
punct |
[,27 |
R3377 |
T12429 |
T12408 |
parataxis |
27,results |
R3378 |
T12430 |
T12429 |
punct |
],27 |
R3379 |
T12431 |
T12408 |
punct |
.,results |
R3380 |
T12433 |
T12434 |
nsubjpass |
Timeless,shown |
R3381 |
T12435 |
T12433 |
punct |
", ",Timeless |
R3382 |
T12436 |
T12437 |
det |
a,factor |
R3383 |
T12437 |
T12433 |
appos |
factor,Timeless |
R3384 |
T12438 |
T12437 |
compound |
transcription,factor |
R3385 |
T12439 |
T12437 |
acl |
involved,factor |
R3386 |
T12440 |
T12439 |
prep |
in,involved |
R3387 |
T12441 |
T12442 |
det |
the,regulation |
R3388 |
T12442 |
T12440 |
pobj |
regulation,in |
R3389 |
T12443 |
T12442 |
prep |
of,regulation |
R3390 |
T12444 |
T12445 |
amod |
circadian,rhythms |
R3391 |
T12445 |
T12443 |
pobj |
rhythms,of |
R3392 |
T12446 |
T12439 |
punct |
", ",involved |
R3393 |
T12447 |
T12439 |
cc |
and,involved |
R3394 |
T12448 |
T12439 |
conj |
expressed,involved |
R3395 |
T12449 |
T12448 |
prep |
in,expressed |
R3396 |
T12450 |
T12451 |
det |
the,tips |
R3397 |
T12451 |
T12449 |
pobj |
tips,in |
R3398 |
T12452 |
T12451 |
amod |
branching,tips |
R3399 |
T12453 |
T12451 |
amod |
ureteric,tips |
R3400 |
T12454 |
T12434 |
punct |
", ",shown |
R3401 |
T12455 |
T12434 |
aux |
has,shown |
R3402 |
T12456 |
T12434 |
advmod |
also,shown |
R3403 |
T12457 |
T12434 |
auxpass |
been,shown |
R3404 |
T12458 |
T12459 |
aux |
to,regulate |
R3405 |
T12459 |
T12434 |
xcomp |
regulate,shown |
R3406 |
T12460 |
T12461 |
amod |
ureteric,morphogenesis |
R3407 |
T12461 |
T12459 |
dobj |
morphogenesis,regulate |
R3408 |
T12462 |
T12461 |
compound |
branching,morphogenesis |
R3409 |
T12463 |
T12464 |
punct |
[,28 |
R3410 |
T12464 |
T12434 |
parataxis |
28,shown |
R3411 |
T12465 |
T12464 |
punct |
],28 |
R3412 |
T12466 |
T12434 |
punct |
.,shown |
R3413 |
T12468 |
T12469 |
nsubj |
We,were |
R3414 |
T12470 |
T12469 |
acomp |
interested,were |
R3415 |
T12471 |
T12470 |
prep |
in,interested |
R3416 |
T12472 |
T12473 |
det |
the,expression |
R3417 |
T12473 |
T12471 |
pobj |
expression,in |
R3418 |
T12474 |
T12473 |
amod |
possible,expression |
R3419 |
T12475 |
T12473 |
amod |
altered,expression |
R3420 |
T12476 |
T12473 |
prep |
of,expression |
R3421 |
T12477 |
T12478 |
advmod |
previously,known |
R3422 |
T12478 |
T12479 |
amod |
known,targets |
R3423 |
T12479 |
T12476 |
pobj |
targets,of |
R3424 |
T12480 |
T12479 |
compound |
Wnt,targets |
R3425 |
T12481 |
T12469 |
prep |
in,were |
R3426 |
T12482 |
T12483 |
det |
the,kidneys |
R3427 |
T12483 |
T12481 |
pobj |
kidneys,in |
R3428 |
T12484 |
T12485 |
nmod |
Pygo1,mutant |
R3429 |
T12485 |
T12483 |
compound |
mutant,kidneys |
R3430 |
T12486 |
T12484 |
punct |
-,Pygo1 |
R3431 |
T12487 |
T12484 |
punct |
/,Pygo1 |
R3432 |
T12488 |
T12484 |
punct |
-,Pygo1 |
R3433 |
T12489 |
T12484 |
punct |
/,Pygo1 |
R3434 |
T12490 |
T12484 |
appos |
Pygo2,Pygo1 |
R3435 |
T12491 |
T12490 |
punct |
-,Pygo2 |
R3436 |
T12492 |
T12490 |
punct |
/,Pygo2 |
R3437 |
T12493 |
T12490 |
punct |
-,Pygo2 |
R3438 |
T12494 |
T12469 |
punct |
.,were |
R3439 |
T12496 |
T12497 |
det |
A,list |
R3440 |
T12497 |
T12498 |
nsubjpass |
list,compiled |
R3441 |
T12499 |
T12497 |
prep |
of,list |
R3442 |
T12500 |
T12501 |
amod |
known,targets |
R3443 |
T12501 |
T12499 |
pobj |
targets,of |
R3444 |
T12502 |
T12501 |
compound |
Wnt,targets |
R3445 |
T12503 |
T12498 |
auxpass |
was,compiled |
R3446 |
T12504 |
T12498 |
prep |
from,compiled |
R3447 |
T12505 |
T12498 |
punct |
.,compiled |
R3448 |
T12507 |
T12508 |
preconj |
Both,Pygo1 |
R3449 |
T12508 |
T12509 |
nmod |
Pygo1,sets |
R3450 |
T12509 |
T12513 |
nsubjpass |
sets,included |
R3451 |
T12510 |
T12508 |
cc |
and,Pygo1 |
R3452 |
T12511 |
T12508 |
conj |
Pygo2,Pygo1 |
R3453 |
T12512 |
T12509 |
compound |
probe,sets |
R3454 |
T12514 |
T12513 |
auxpass |
were,included |
R3455 |
T12515 |
T12513 |
prep |
as,included |
R3456 |
T12516 |
T12515 |
pobj |
references,as |
R3457 |
T12517 |
T12518 |
aux |
to,illustrate |
R3458 |
T12518 |
T12513 |
advcl |
illustrate,included |
R3459 |
T12519 |
T12520 |
amod |
significant,regulation |
R3460 |
T12520 |
T12518 |
dobj |
regulation,illustrate |
R3461 |
T12521 |
T12520 |
amod |
down,regulation |
R3462 |
T12522 |
T12513 |
punct |
.,included |
R3463 |
T12524 |
T12525 |
advmod |
Remarkably,showed |
R3464 |
T12526 |
T12525 |
punct |
", ",showed |
R3465 |
T12527 |
T12528 |
det |
the,genes |
R3466 |
T12528 |
T12525 |
nsubj |
genes,showed |
R3467 |
T12529 |
T12528 |
amod |
known,genes |
R3468 |
T12530 |
T12531 |
compound |
Wnt,target |
R3469 |
T12531 |
T12528 |
compound |
target,genes |
R3470 |
T12532 |
T12533 |
advmod |
very,few |
R3471 |
T12533 |
T12534 |
amod |
few,differences |
R3472 |
T12534 |
T12525 |
dobj |
differences,showed |
R3473 |
T12535 |
T12534 |
prep |
in,differences |
R3474 |
T12536 |
T12535 |
pobj |
expression,in |
R3475 |
T12537 |
T12534 |
prep |
between,differences |
R3476 |
T12538 |
T12539 |
amod |
wild,type |
R3477 |
T12539 |
T12541 |
nmod |
type,kidneys |
R3478 |
T12540 |
T12539 |
punct |
-,type |
R3479 |
T12541 |
T12537 |
pobj |
kidneys,between |
R3480 |
T12542 |
T12539 |
cc |
and,type |
R3481 |
T12543 |
T12539 |
conj |
mutant,type |
R3482 |
T12544 |
T12545 |
punct |
(,Figure |
R3483 |
T12545 |
T12525 |
parataxis |
Figure,showed |
R3484 |
T12546 |
T12545 |
nummod |
8,Figure |
R3485 |
T12547 |
T12545 |
punct |
),Figure |
R3486 |
T12548 |
T12525 |
punct |
.,showed |
R3487 |
T12550 |
T12551 |
advmod |
Only,expression |
R3488 |
T12551 |
T12553 |
nsubjpass |
expression,changed |
R3489 |
T12552 |
T12551 |
det |
the,expression |
R3490 |
T12554 |
T12551 |
prep |
of,expression |
R3491 |
T12555 |
T12556 |
npadvmod |
Ccnd1,encoding |
R3492 |
T12556 |
T12557 |
amod |
encoding,D1 |
R3493 |
T12557 |
T12554 |
pobj |
D1,of |
R3494 |
T12558 |
T12557 |
compound |
cyclin,D1 |
R3495 |
T12559 |
T12560 |
punct |
[,29 |
R3496 |
T12560 |
T12557 |
parataxis |
29,D1 |
R3497 |
T12561 |
T12560 |
punct |
],29 |
R3498 |
T12562 |
T12557 |
punct |
", ",D1 |
R3499 |
T12563 |
T12557 |
cc |
and,D1 |
R3500 |
T12564 |
T12557 |
conj |
Wisp1,D1 |
R3501 |
T12565 |
T12566 |
punct |
[,30 |
R3502 |
T12566 |
T12564 |
parataxis |
30,Wisp1 |
R3503 |
T12567 |
T12566 |
punct |
],30 |
R3504 |
T12568 |
T12553 |
auxpass |
were,changed |
R3505 |
T12569 |
T12553 |
advmod |
significantly,changed |
R3506 |
T12570 |
T12553 |
punct |
", ",changed |
R3507 |
T12571 |
T12553 |
prep |
with,changed |
R3508 |
T12572 |
T12573 |
compound |
expression,level |
R3509 |
T12573 |
T12574 |
compound |
level,changes |
R3510 |
T12574 |
T12571 |
pobj |
changes,with |
R3511 |
T12575 |
T12574 |
prep |
of,changes |
R3512 |
T12576 |
T12577 |
punct |
<,1.5-fold |
R3513 |
T12577 |
T12575 |
pobj |
1.5-fold,of |
R3514 |
T12578 |
T12574 |
prep |
in,changes |
R3515 |
T12579 |
T12580 |
compound |
Pygo1,Pygo2 |
R3516 |
T12580 |
T12582 |
npadvmod |
Pygo2,null |
R3517 |
T12581 |
T12580 |
punct |
/,Pygo2 |
R3518 |
T12582 |
T12583 |
amod |
null,kidneys |
R3519 |
T12583 |
T12578 |
pobj |
kidneys,in |
R3520 |
T12584 |
T12553 |
punct |
.,changed |
R3521 |
T12586 |
T12587 |
det |
These,results |
R3522 |
T12587 |
T12588 |
nsubj |
results,indicate |
R3523 |
T12589 |
T12590 |
det |
an,absence |
R3524 |
T12590 |
T12588 |
dobj |
absence,indicate |
R3525 |
T12591 |
T12590 |
prep |
of,absence |
R3526 |
T12592 |
T12593 |
amod |
dramatic,changes |
R3527 |
T12593 |
T12591 |
pobj |
changes,of |
R3528 |
T12594 |
T12593 |
prep |
in,changes |
R3529 |
T12595 |
T12594 |
pobj |
expression,in |
R3530 |
T12596 |
T12595 |
prep |
of,expression |
R3531 |
T12597 |
T12598 |
advmod |
previously,known |
R3532 |
T12598 |
T12599 |
amod |
known,genes |
R3534 |
T12600 |
T12601 |
compound |
Wnt,signaling |
R3573 |
T12643 |
T12644 |
det |
a,change |
R3574 |
T12644 |
T12639 |
dobj |
change,observe |
R3575 |
T12645 |
T12644 |
amod |
significant,change |
R3576 |
T12646 |
T12644 |
compound |
fold,change |
R3577 |
T12647 |
T12644 |
prep |
in,change |
R3578 |
T12648 |
T12649 |
det |
the,direction |
R3579 |
T12649 |
T12647 |
pobj |
direction,in |
R3580 |
T12650 |
T12649 |
amod |
same,direction |
R3581 |
T12651 |
T12649 |
acl |
predicted,direction |
R3582 |
T12652 |
T12651 |
agent |
by,predicted |
R3583 |
T12653 |
T12654 |
det |
the,results |
R3584 |
T12654 |
T12652 |
pobj |
results,by |
R3585 |
T12655 |
T12654 |
compound |
microarray,results |
R3586 |
T12656 |
T12657 |
punct |
(,Table |
R3587 |
T12657 |
T12639 |
parataxis |
Table,observe |
R3588 |
T12658 |
T12657 |
nummod |
4,Table |
R3589 |
T12659 |
T12657 |
punct |
),Table |
R3590 |
T12660 |
T12639 |
punct |
.,observe |
R3591 |
T12662 |
T12663 |
nsubj |
It,is |
R3592 |
T12664 |
T12665 |
neg |
not,surprising |
R3593 |
T12665 |
T12663 |
acomp |
surprising,is |
R3594 |
T12666 |
T12667 |
mark |
that,validated |
R3595 |
T12667 |
T12663 |
ccomp |
validated,is |
R3596 |
T12668 |
T12667 |
nsubjpass |
two,validated |
R3597 |
T12669 |
T12668 |
prep |
of,two |
R3598 |
T12670 |
T12671 |
det |
the,genes |
R3599 |
T12671 |
T12669 |
pobj |
genes,of |
R3600 |
T12672 |
T12671 |
nummod |
nine,genes |
R3601 |
T12673 |
T12667 |
aux |
could,validated |
R3602 |
T12674 |
T12667 |
neg |
not,validated |
R3603 |
T12675 |
T12667 |
auxpass |
be,validated |
R3604 |
T12676 |
T12663 |
punct |
", ",is |
R3605 |
T12677 |
T12663 |
advcl |
considering,is |
R3606 |
T12678 |
T12679 |
det |
the,stringency |
R3607 |
T12679 |
T12677 |
dobj |
stringency,considering |
R3608 |
T12680 |
T12681 |
advmod |
relatively,low |
R3609 |
T12681 |
T12679 |
amod |
low,stringency |
R3610 |
T12682 |
T12679 |
acl |
used,stringency |
R3611 |
T12683 |
T12682 |
prep |
in,used |
R3612 |
T12684 |
T12683 |
pcomp |
screening,in |
R3613 |
T12685 |
T12686 |
det |
the,data |
R3614 |
T12686 |
T12684 |
dobj |
data,screening |
R3615 |
T12687 |
T12686 |
compound |
microarray,data |
R3616 |
T12688 |
T12663 |
punct |
.,is |
R7885 |
T27272 |
T27273 |
compound |
Gene,expression |
R7886 |
T27273 |
T27274 |
compound |
expression,changes |
R7887 |
T27275 |
T27274 |
prep |
of,changes |
R7888 |
T27276 |
T27277 |
amod |
common,targets |
R7889 |
T27277 |
T27275 |
pobj |
targets,of |
R7890 |
T27278 |
T27277 |
compound |
Wnt,targets |
R7891 |
T27279 |
T27277 |
compound |
signaling,targets |
R7892 |
T27280 |
T27274 |
prep |
in,changes |
R7893 |
T27281 |
T27282 |
det |
the,kidney |
R7894 |
T27282 |
T27280 |
pobj |
kidney,in |
R7895 |
T27283 |
T27282 |
nmod |
E18.5,kidney |
R7896 |
T27284 |
T27285 |
compound |
Pygo1,Pygo2 |
R7897 |
T27285 |
T27287 |
npadvmod |
Pygo2,null |
R7898 |
T27286 |
T27285 |
punct |
/,Pygo2 |
R7899 |
T27287 |
T27282 |
amod |
null,kidney |
R7900 |
T27288 |
T27274 |
punct |
.,changes |
R7901 |
T27290 |
T27291 |
compound |
Microarray,analysis |
R7902 |
T27291 |
T27292 |
nsubjpass |
analysis,performed |
R7903 |
T27293 |
T27292 |
auxpass |
was,performed |
R7904 |
T27294 |
T27292 |
prep |
in,performed |
R7905 |
T27295 |
T27294 |
pobj |
triplicate,in |
R7906 |
T27296 |
T27292 |
prep |
on,performed |
R7907 |
T27297 |
T27298 |
amod |
wild,type |
R7908 |
T27298 |
T27300 |
nmod |
type,kidneys |
R7909 |
T27299 |
T27298 |
punct |
-,type |
R7910 |
T27300 |
T27296 |
pobj |
kidneys,on |
R7911 |
T27301 |
T27298 |
cc |
and,type |
R7912 |
T27302 |
T27303 |
npadvmod |
Pygo1,null |
R7913 |
T27303 |
T27298 |
conj |
null,type |
R7914 |
T27304 |
T27302 |
punct |
/,Pygo1 |
R7915 |
T27305 |
T27302 |
nummod |
2,Pygo1 |
R7916 |
T27306 |
T27303 |
npadvmod |
compound,null |
R7917 |
T27307 |
T27300 |
compound |
E18.5,kidneys |
R7918 |
T27308 |
T27292 |
punct |
.,performed |
R7919 |
T27310 |
T27311 |
amod |
Possible,targets |
R7920 |
T27311 |
T27313 |
nsubjpass |
targets,selected |
R7921 |
T27312 |
T27311 |
compound |
Wnt,targets |
R7922 |
T27314 |
T27313 |
auxpass |
were,selected |
R7923 |
T27315 |
T27313 |
prep |
from,selected |
R7924 |
T27316 |
T27315 |
pobj |
those,from |
R7925 |
T27317 |
T27316 |
acl |
compiled,those |
R7926 |
T27318 |
T27317 |
prep |
at,compiled |
R7927 |
T27319 |
T27318 |
punct |
[,at |
R7928 |
T27320 |
T27318 |
pobj |
39,at |
R7929 |
T27321 |
T27313 |
punct |
],selected |
R7930 |
T27322 |
T27313 |
punct |
.,selected |
R7931 |
T27324 |
T27325 |
det |
An,list |
R7932 |
T27325 |
T27328 |
nsubjpass |
list,created |
R7933 |
T27326 |
T27325 |
amod |
initial,list |
R7934 |
T27327 |
T27325 |
compound |
gene,list |
R7935 |
T27329 |
T27325 |
prep |
of,list |
R7936 |
T27330 |
T27331 |
nummod |
82,targets |
R7937 |
T27331 |
T27329 |
pobj |
targets,of |
R7938 |
T27332 |
T27331 |
compound |
Wnt,targets |
R7939 |
T27333 |
T27328 |
auxpass |
was,created |
R7940 |
T27334 |
T27328 |
cc |
and,created |
R7941 |
T27335 |
T27336 |
advmod |
then,reduced |
R7942 |
T27336 |
T27328 |
conj |
reduced,created |
R7943 |
T27337 |
T27336 |
prep |
to,reduced |
R7944 |
T27338 |
T27339 |
det |
a,total |
R7945 |
T27339 |
T27337 |
pobj |
total,to |
R7946 |
T27340 |
T27339 |
prep |
of,total |
R7947 |
T27341 |
T27342 |
nummod |
40,genes |
R7948 |
T27342 |
T27340 |
pobj |
genes,of |
R7949 |
T27343 |
T27336 |
advcl |
using,reduced |
R7950 |
T27344 |
T27345 |
det |
an,restriction |
R7951 |
T27345 |
T27343 |
dobj |
restriction,using |
R7952 |
T27346 |
T27347 |
compound |
expression,level |
R7953 |
T27347 |
T27345 |
compound |
level,restriction |
R7954 |
T27348 |
T27345 |
acl |
requiring,restriction |
R7955 |
T27349 |
T27350 |
det |
the,intensity |
R7956 |
T27350 |
T27348 |
dobj |
intensity,requiring |
R7957 |
T27351 |
T27350 |
amod |
raw,intensity |
R7958 |
T27352 |
T27350 |
compound |
expression,intensity |
R7959 |
T27353 |
T27354 |
aux |
to,be |
R7960 |
T27354 |
T27348 |
xcomp |
be,requiring |
R7961 |
T27355 |
T27356 |
punct |
>,100 |
R7962 |
T27356 |
T27354 |
attr |
100,be |
R7963 |
T27357 |
T27354 |
prep |
in,be |
R7964 |
T27358 |
T27359 |
advmod |
at,3 |
R7965 |
T27359 |
T27361 |
nummod |
3,samples |
R7966 |
T27360 |
T27359 |
advmod |
least,3 |
R7967 |
T27361 |
T27357 |
pobj |
samples,in |
R7968 |
T27362 |
T27328 |
punct |
.,created |
R7969 |
T27364 |
T27365 |
nmod |
Pygo1,probes |
R7970 |
T27365 |
T27368 |
nsubjpass |
probes,included |
R7971 |
T27366 |
T27364 |
cc |
and,Pygo1 |
R7972 |
T27367 |
T27364 |
conj |
Pygo2,Pygo1 |
R7973 |
T27369 |
T27368 |
auxpass |
were,included |
R7974 |
T27370 |
T27371 |
aux |
to,demonstrate |
R7975 |
T27371 |
T27368 |
advcl |
demonstrate,included |
R7976 |
T27372 |
T27373 |
amod |
significant,changes |
R7977 |
T27373 |
T27371 |
dobj |
changes,demonstrate |
R7978 |
T27374 |
T27373 |
prep |
in,changes |
R7979 |
T27375 |
T27376 |
compound |
expression,levels |
R7980 |
T27376 |
T27374 |
pobj |
levels,in |
R7981 |
T27377 |
T27368 |
punct |
.,included |
R8019 |
T27573 |
T27574 |
advmod |
differentially,expressed |
R8020 |
T27574 |
T27572 |
acl |
expressed,Genes |
R8021 |
T27575 |
T27576 |
punct |
(,change |
R8022 |
T27576 |
T27574 |
parataxis |
change,expressed |
R8023 |
T27577 |
T27578 |
nsubj |
p,0.05 |
R8024 |
T27578 |
T27576 |
ccomp |
0.05,change |
R8025 |
T27579 |
T27578 |
punct |
<,0.05 |
R8026 |
T27580 |
T27576 |
punct |
", ",change |
R8027 |
T27581 |
T27576 |
amod |
two-fold,change |
R8028 |
T27582 |
T27583 |
cc |
or,greater |
R8029 |
T27583 |
T27576 |
nummod |
greater,change |
R8030 |
T27584 |
T27576 |
punct |
),change |
R8031 |
T27585 |
T27574 |
prep |
in,expressed |
R8032 |
T27586 |
T27587 |
det |
the,kidney |
R8033 |
T27587 |
T27585 |
pobj |
kidney,in |
R8034 |
T27588 |
T27589 |
compound |
Pygo1,Pygo2 |
R8035 |
T27589 |
T27591 |
npadvmod |
Pygo2,null |
R8036 |
T27590 |
T27589 |
punct |
/,Pygo2 |
R8037 |
T27591 |
T27587 |
amod |
null,kidney |
R8038 |
T27592 |
T27587 |
compound |
E18.5,kidney |
R8039 |
T27593 |
T27587 |
acl |
normalized,kidney |
R8040 |
T27594 |
T27593 |
prep |
to,normalized |
R8041 |
T27595 |
T27596 |
amod |
wild,type |
R8042 |
T27596 |
T27598 |
compound |
type,samples |
R8043 |
T27597 |
T27596 |
punct |
-,type |
R8044 |
T27598 |
T27594 |
pobj |
samples,to |
R8045 |
T27599 |
T27572 |
punct |
.,Genes |
R8046 |
T27656 |
T27655 |
prep |
of,Validation |
R8047 |
T27657 |
T27658 |
compound |
gene,expression |
R8048 |
T27658 |
T27659 |
compound |
expression,changes |
R8049 |
T27659 |
T27656 |
pobj |
changes,of |
R8050 |
T27660 |
T27659 |
prep |
in,changes |
R8051 |
T27661 |
T27662 |
det |
the,kidneys |
R8052 |
T27662 |
T27660 |
pobj |
kidneys,in |
R8053 |
T27663 |
T27662 |
nmod |
E18.5,kidneys |
R8054 |
T27664 |
T27665 |
compound |
Pygo1,Pygo2 |
R8055 |
T27665 |
T27667 |
npadvmod |
Pygo2,null |
R8056 |
T27666 |
T27665 |
punct |
/,Pygo2 |
R8057 |
T27667 |
T27662 |
amod |
null,kidneys |
R8058 |
T27668 |
T27659 |
acl |
normalized,changes |
R8059 |
T27669 |
T27668 |
prep |
to,normalized |
R8060 |
T27670 |
T27671 |
nmod |
E18.5,kidneys |
R8061 |
T27671 |
T27669 |
pobj |
kidneys,to |
R8062 |
T27672 |
T27673 |
amod |
wild,type |
R8063 |
T27673 |
T27671 |
compound |
type,kidneys |
R8064 |
T27674 |
T27673 |
punct |
-,type |
R8065 |
T27675 |
T27655 |
punct |
.,Validation |
R3238 |
T12277 |
T12271 |
advmod |
strongly,expressed |
R3239 |
T12278 |
T12271 |
prep |
in,expressed |
R3240 |
T12279 |
T12280 |
det |
the,tubules |
R3241 |
T12280 |
T12278 |
pobj |
tubules,in |
R3242 |
T12281 |
T12280 |
prep |
of,tubules |
R3243 |
T12282 |
T12283 |
det |
the,kidney |
R3244 |
T12283 |
T12281 |
pobj |
kidney,of |
R3245 |
T12284 |
T12283 |
amod |
adult,kidney |
R3246 |
T12285 |
T12286 |
punct |
[,24 |
R3247 |
T12286 |
T12271 |
parataxis |
24,expressed |
R3248 |
T12287 |
T12286 |
punct |
],24 |
R3249 |
T12288 |
T12271 |
punct |
.,expressed |
R3250 |
T12290 |
T12291 |
det |
These,results |
R3251 |
T12291 |
T12292 |
nsubj |
results,suggest |
R3252 |
T12293 |
T12294 |
mark |
that,play |
R3253 |
T12294 |
T12292 |
ccomp |
play,suggest |
R3254 |
T12295 |
T12296 |
det |
the,genes |
R3255 |
T12296 |
T12294 |
nsubj |
genes,play |
R3256 |
T12297 |
T12298 |
compound |
Pygo1,Pygo2 |
R3257 |
T12298 |
T12296 |
compound |
Pygo2,genes |
R3258 |
T12299 |
T12298 |
punct |
/,Pygo2 |
R3259 |
T12300 |
T12294 |
aux |
might,play |
R3260 |
T12301 |
T12302 |
det |
a,role |
R3261 |
T12302 |
T12294 |
dobj |
role,play |
R3262 |
T12303 |
T12294 |
prep |
in,play |
R3263 |
T12304 |
T12305 |
compound |
nephron,maturation |
R3264 |
T12305 |
T12303 |
pobj |
maturation,in |
R3265 |
T12306 |
T12305 |
cc |
and,maturation |
R3533 |
T12599 |
T12596 |
pobj |
genes,of |
R3535 |
T12601 |
T12599 |
compound |
signaling,genes |
R3536 |
T12602 |
T12599 |
compound |
target,genes |
R3537 |
T12603 |
T12595 |
prep |
in,expression |
R3538 |
T12604 |
T12605 |
det |
the,kidneys |
R3539 |
T12605 |
T12603 |
pobj |
kidneys,in |
R3540 |
T12606 |
T12607 |
compound |
Pygo1,Pygo2 |
R3541 |
T12607 |
T12609 |
compound |
Pygo2,mutant |
R3542 |
T12608 |
T12607 |
punct |
/,Pygo2 |
R3543 |
T12609 |
T12605 |
compound |
mutant,kidneys |
R3544 |
T12610 |
T12588 |
punct |
.,indicate |
R3545 |
T12612 |
T12613 |
nsubj |
We,used |
R3546 |
T12614 |
T12615 |
amod |
quantitative,PCR |
R3547 |
T12615 |
T12613 |
dobj |
PCR,used |
R3548 |
T12616 |
T12617 |
amod |
real,time |
R3549 |
T12617 |
T12615 |
compound |
time,PCR |
R3550 |
T12618 |
T12617 |
punct |
-,time |
R3551 |
T12619 |
T12615 |
punct |
", ",PCR |
R3552 |
T12620 |
T12615 |
prep |
with,PCR |
R3553 |
T12621 |
T12622 |
amod |
independent,samples |
R3554 |
T12622 |
T12620 |
pobj |
samples,with |
R3555 |
T12623 |
T12622 |
amod |
biological,samples |
R3556 |
T12624 |
T12613 |
punct |
", ",used |
R3557 |
T12625 |
T12626 |
aux |
to,validate |
R3558 |
T12626 |
T12613 |
advcl |
validate,used |
R3559 |
T12627 |
T12628 |
det |
the,results |
R3560 |
T12628 |
T12626 |
dobj |
results,validate |
R3561 |
T12629 |
T12628 |
compound |
microarray,results |
R3562 |
T12630 |
T12613 |
punct |
.,used |
R3563 |
T12632 |
T12633 |
nummod |
Nine,genes |
R3564 |
T12633 |
T12634 |
nsubjpass |
genes,tested |
R3565 |
T12635 |
T12634 |
auxpass |
were,tested |
R3566 |
T12636 |
T12634 |
punct |
", ",tested |
R3567 |
T12637 |
T12634 |
cc |
and,tested |
R3568 |
T12638 |
T12639 |
prep |
for,observe |
R3569 |
T12639 |
T12634 |
conj |
observe,tested |
R3570 |
T12640 |
T12638 |
pobj |
seven,for |
R3571 |
T12641 |
T12639 |
nsubj |
we,observe |
R3572 |
T12642 |
T12639 |
aux |
did,observe |