PMC:1853120 / 51462-52238 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/1853120","sourcedb":"PMC","sourceid":"1853120","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1853120","text":"(A) Diagram of targeting strategy. Homologous recombination of the targeting vector with the wild-type Dmrt7 allele (Dmrt7+) resulted in the targeted allele Dmrtneo. This allele contains loxP sites flanking the translational start and the second and third exons (containing the DM domain, gray), as well as a neomycine-resistance cassette flanked by Flp recombinase recognition sites (frt sites). Mice heterozygous for the Dmrt1neo allele were mated with transgenic mice expressing Cre recombinase, resulting in deletion of the sequence between the two loxP sites, including the neo cassette. The resulting deletion allele is called Dmrt7−. Mating of Dmrt7neo mice with transgenic mice expressing the Flpe recombinase excised the neo cassette, generating the Dmrt7flox allele.","tracks":[]}