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polyclonal antibodies to DMRT7 were raised against a purified fusion protein containing glutathione-S-transferase (GST) fused to the C-terminal 279 amino acids of DMRT7. Antibodies to GST were removed by GST-affigel 10 chromatography and the antiserum was then purified by GST-DMRT7-affigel 15 chromatography. DMRT7 antibody was used at 1:200 dilution with a goat anti-rabbit secondary antibody (Molecular Probes, http://www.invitrogen.com) at 1:200 dilution. Other primary antibodies used for immunofluorescence were rat anti-GATA1 (1:200, Santa Cruz Biotechnology, http://www.scbt.com, sc-265), goat anti-GATA4 (1:200, Santa Cruz Biotechnology, sc-1237), rat anti-TRA98 (1:200, gift of H. Tanaka and Y. Nishimune), rat anti-BC7 (1:50, gift of H. Tanaka and Y. Nishimune), rat anti-TRA369 (1:200, gift of H. Tanaka and Y. Nishimune), rabbit anti-RAD51 (1:600 Calbiochem, http://www.calbiochem.com, PC130), mouse anti-GMP-1/SUMO-1 (1:200, Zymed, http://invitrogen.com, 33–2400), rabbit anti-phospho-H2AX (Ser139) (1:200, Upstate, http://www.millipore.com, 01–164), mouse anti-phospho-H2AX (1:200, Upstate, 05–636), mouse anti-SYCP3 (1:200, Abcam, http://www.abcam.com, ab12452), rabbit anti-HP1β (1:100, Abcam, ab10478), rabbit anti-H3-2meK9 (1:100, Upstate, 07–441), rabbit anti-H3-3meK9 (1:200, Upstate, 07–442), rabbit anti-AR (N-20) (1:200, Santa Cruz Biotechnology, sc-816), and mouse anti-αSMA clone 1A4 (1:800, Sigma, http://www.sigmaaldrich.com, A2547). Secondary antibodies used were goat anti-rabbit Alexa 488, goat anti-rabbit Alexa 594, goat anti-rat Alexa 594, and goat anti-mouse Alexa 488 (Molecular Probes) used at 1:250. Donkey anti-goat FITC (Jackson ImmunoResearch Laboratories, http://www.jacksonimmuno.com) and donkey anti-rabbit Texas Red (Jackson) were used at 1:50 according to the manufacturer's instructions."}

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Antibodies to GST were removed by GST-affigel 10 chromatography and the antiserum was then purified by GST-DMRT7-affigel 15 chromatography. DMRT7 antibody was used at 1:200 dilution with a goat anti-rabbit secondary antibody (Molecular Probes, http://www.invitrogen.com) at 1:200 dilution. Other primary antibodies used for immunofluorescence were rat anti-GATA1 (1:200, Santa Cruz Biotechnology, http://www.scbt.com, sc-265), goat anti-GATA4 (1:200, Santa Cruz Biotechnology, sc-1237), rat anti-TRA98 (1:200, gift of H. Tanaka and Y. Nishimune), rat anti-BC7 (1:50, gift of H. Tanaka and Y. Nishimune), rat anti-TRA369 (1:200, gift of H. Tanaka and Y. Nishimune), rabbit anti-RAD51 (1:600 Calbiochem, http://www.calbiochem.com, PC130), mouse anti-GMP-1/SUMO-1 (1:200, Zymed, http://invitrogen.com, 33–2400), rabbit anti-phospho-H2AX (Ser139) (1:200, Upstate, http://www.millipore.com, 01–164), mouse anti-phospho-H2AX (1:200, Upstate, 05–636), mouse anti-SYCP3 (1:200, Abcam, http://www.abcam.com, ab12452), rabbit anti-HP1β (1:100, Abcam, ab10478), rabbit anti-H3-2meK9 (1:100, Upstate, 07–441), rabbit anti-H3-3meK9 (1:200, Upstate, 07–442), rabbit anti-AR (N-20) (1:200, Santa Cruz Biotechnology, sc-816), and mouse anti-αSMA clone 1A4 (1:800, Sigma, http://www.sigmaaldrich.com, A2547). Secondary antibodies used were goat anti-rabbit Alexa 488, goat anti-rabbit Alexa 594, goat anti-rat Alexa 594, and goat anti-mouse Alexa 488 (Molecular Probes) used at 1:250. Donkey anti-goat FITC (Jackson ImmunoResearch Laboratories, http://www.jacksonimmuno.com) and donkey anti-rabbit Texas Red (Jackson) were used at 1:50 according to the manufacturer's instructions."}

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polyclonal antibodies to DMRT7 were raised against a purified fusion protein containing glutathione-S-transferase (GST) fused to the C-terminal 279 amino acids of DMRT7. Antibodies to GST were removed by GST-affigel 10 chromatography and the antiserum was then purified by GST-DMRT7-affigel 15 chromatography. DMRT7 antibody was used at 1:200 dilution with a goat anti-rabbit secondary antibody (Molecular Probes, http://www.invitrogen.com) at 1:200 dilution. Other primary antibodies used for immunofluorescence were rat anti-GATA1 (1:200, Santa Cruz Biotechnology, http://www.scbt.com, sc-265), goat anti-GATA4 (1:200, Santa Cruz Biotechnology, sc-1237), rat anti-TRA98 (1:200, gift of H. Tanaka and Y. Nishimune), rat anti-BC7 (1:50, gift of H. Tanaka and Y. Nishimune), rat anti-TRA369 (1:200, gift of H. Tanaka and Y. Nishimune), rabbit anti-RAD51 (1:600 Calbiochem, http://www.calbiochem.com, PC130), mouse anti-GMP-1/SUMO-1 (1:200, Zymed, http://invitrogen.com, 33–2400), rabbit anti-phospho-H2AX (Ser139) (1:200, Upstate, http://www.millipore.com, 01–164), mouse anti-phospho-H2AX (1:200, Upstate, 05–636), mouse anti-SYCP3 (1:200, Abcam, http://www.abcam.com, ab12452), rabbit anti-HP1β (1:100, Abcam, ab10478), rabbit anti-H3-2meK9 (1:100, Upstate, 07–441), rabbit anti-H3-3meK9 (1:200, Upstate, 07–442), rabbit anti-AR (N-20) (1:200, Santa Cruz Biotechnology, sc-816), and mouse anti-αSMA clone 1A4 (1:800, Sigma, http://www.sigmaaldrich.com, A2547). Secondary antibodies used were goat anti-rabbit Alexa 488, goat anti-rabbit Alexa 594, goat anti-rat Alexa 594, and goat anti-mouse Alexa 488 (Molecular Probes) used at 1:250. Donkey anti-goat FITC (Jackson ImmunoResearch Laboratories, http://www.jacksonimmuno.com) and donkey anti-rabbit Texas Red (Jackson) were used at 1:50 according to the manufacturer's instructions."}