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The targeting vector pDZ169 (diagrammed in Figure S2) was constructed by the following scheme: The vector pDZ157 was used as a backbone vector [31]. 3′ to Pgk-neo and the loxP site, we inserted, as a XmaI/XmaI DNA fragment, the third intron of Dmrt7 (from 366 bp to 2,773 bp downstream of exon 3) generated by PCR. 5′ to Pgk-neo, we inserted an EcoRI/NotI PCR fragment extending from 4,107 bp to 336 bp 5′ of the Dmrt7 translational start. Finally, we inserted a loxP site and NotI site 336 bp 5′ of the Dmrt7 translational start. In the resulting vector, the second and third exons of Dmrt7 are flanked by loxP sites (floxed). The Dmrt7-containing portions of pDZ169 were completely sequenced."}

{"project":"2_test","denotations":[{"id":"17447844-11040213-85805865","span":{"begin":412,"end":414},"obj":"11040213"},{"id":"T5457","span":{"begin":412,"end":414},"obj":"11040213"}],"text":"A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to screen a mouse BAC library from the 129/SvJ strain (Stratagene, http://www.stratagene.com), and clones containing promoter sequences were isolated and sequenced to obtain Dmrt7 genomic sequence. The targeting vector pDZ169 (diagrammed in Figure S2) was constructed by the following scheme: The vector pDZ157 was used as a backbone vector [31]. 3′ to Pgk-neo and the loxP site, we inserted, as a XmaI/XmaI DNA fragment, the third intron of Dmrt7 (from 366 bp to 2,773 bp downstream of exon 3) generated by PCR. 5′ to Pgk-neo, we inserted an EcoRI/NotI PCR fragment extending from 4,107 bp to 336 bp 5′ of the Dmrt7 translational start. Finally, we inserted a loxP site and NotI site 336 bp 5′ of the Dmrt7 translational start. In the resulting vector, the second and third exons of Dmrt7 are flanked by loxP sites (floxed). The Dmrt7-containing portions of pDZ169 were completely sequenced."}

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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T23703","span":{"begin":404,"end":410},"obj":"SO_EXT:0000440"},{"id":"T23688","span":{"begin":2,"end":7},"obj":"NCBITaxon:10088"},{"id":"T23689","span":{"begin":8,"end":13},"obj":"PR_EXT:000006549"},{"id":"T23690","span":{"begin":14,"end":18},"obj":"SO_EXT:cDNA"},{"id":"T23691","span":{"begin":39,"end":48},"obj":"SO_EXT:biological_sequence"},{"id":"T23692","span":{"begin":54,"end":58},"obj":"SO_EXT:0000147"},{"id":"T23693","span":{"begin":82,"end":87},"obj":"NCBITaxon:10088"},{"id":"T23694","span":{"begin":88,"end":91},"obj":"SO_EXT:0000153"},{"id":"T23695","span":{"begin":169,"end":175},"obj":"SO_EXT:sequence_cloned_entity"},{"id":"T23696","span":{"begin":187,"end":195},"obj":"SO_EXT:0000167"},{"id":"T23697","span":{"begin":196,"end":205},"obj":"SO_EXT:biological_sequence"},{"id":"T23698","span":{"begin":244,"end":249},"obj":"PR_EXT:000006549"},{"id":"T23699","span":{"begin":250,"end":257},"obj":"SO_EXT:0001026"},{"id":"T23700","span":{"begin":258,"end":266},"obj":"SO_EXT:biological_sequence"},{"id":"T23701","span":{"begin":272,"end":288},"obj":"SO_EXT:0001644"},{"id":"T23702","span":{"begin":367,"end":373},"obj":"SO_EXT:0000440"},{"id":"T23704","span":{"begin":423,"end":426},"obj":"GO_EXT:phosphoglycerate_kinase"},{"id":"T23705","span":{"begin":427,"end":430},"obj":"CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase"},{"id":"T23706","span":{"begin":439,"end":448},"obj":"SO_EXT:0000346"},{"id":"T23707","span":{"begin":453,"end":461},"obj":"SO_EXT:sequence_insertion_process"},{"id":"T23708","span":{"begin":478,"end":481},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T23709","span":{"begin":502,"end":508},"obj":"SO_EXT:0000188"},{"id":"T23710","span":{"begin":512,"end":517},"obj":"PR_EXT:000006549"},{"id":"T23711","span":{"begin":528,"end":530},"obj":"SO_EXT:0000028"},{"id":"T23712","span":{"begin":540,"end":542},"obj":"SO_EXT:0000028"},{"id":"T23713","span":{"begin":543,"end":553},"obj":"SO_EXT:sequence_downstreamness"},{"id":"T23714","span":{"begin":557,"end":561},"obj":"SO_EXT:0000147"},{"id":"T23715","span":{"begin":589,"end":592},"obj":"GO_EXT:phosphoglycerate_kinase"},{"id":"T23716","span":{"begin":593,"end":596},"obj":"CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase"},{"id":"T23717","span":{"begin":601,"end":609},"obj":"SO_EXT:sequence_insertion_process"},{"id":"T23718","span":{"begin":658,"end":660},"obj":"SO_EXT:0000028"},{"id":"T23719","span":{"begin":668,"end":670},"obj":"SO_EXT:0000028"},{"id":"T23720","span":{"begin":681,"end":686},"obj":"PR_EXT:000006549"},{"id":"T23721","span":{"begin":687,"end":706},"obj":"GO_SO_EXT:translational_start_entity_or_process"},{"id":"T23722","span":{"begin":720,"end":728},"obj":"SO_EXT:sequence_insertion_process"},{"id":"T23723","span":{"begin":731,"end":740},"obj":"SO_EXT:0000346"},{"id":"T23724","span":{"begin":750,"end":754},"obj":"SO_EXT:0000061"},{"id":"T23725","span":{"begin":759,"end":761},"obj":"SO_EXT:0000028"},{"id":"T23726","span":{"begin":772,"end":777},"obj":"PR_EXT:000006549"},{"id":"T23727","span":{"begin":778,"end":797},"obj":"GO_SO_EXT:translational_start_entity_or_process"},{"id":"T23728","span":{"begin":816,"end":822},"obj":"SO_EXT:0000440"},{"id":"T23729","span":{"begin":845,"end":850},"obj":"SO_EXT:0000147"},{"id":"T23730","span":{"begin":854,"end":859},"obj":"PR_EXT:000006549"},{"id":"T23731","span":{"begin":864,"end":871},"obj":"SO:0000357"},{"id":"T23732","span":{"begin":875,"end":885},"obj":"SO_EXT:0000346"},{"id":"T23733","span":{"begin":887,"end":893},"obj":"SO:0000359"},{"id":"T23734","span":{"begin":900,"end":905},"obj":"PR_EXT:000006549"}],"text":"A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to screen a mouse BAC library from the 129/SvJ strain (Stratagene, http://www.stratagene.com), and clones containing promoter sequences were isolated and sequenced to obtain Dmrt7 genomic sequence. The targeting vector pDZ169 (diagrammed in Figure S2) was constructed by the following scheme: The vector pDZ157 was used as a backbone vector [31]. 3′ to Pgk-neo and the loxP site, we inserted, as a XmaI/XmaI DNA fragment, the third intron of Dmrt7 (from 366 bp to 2,773 bp downstream of exon 3) generated by PCR. 5′ to Pgk-neo, we inserted an EcoRI/NotI PCR fragment extending from 4,107 bp to 336 bp 5′ of the Dmrt7 translational start. Finally, we inserted a loxP site and NotI site 336 bp 5′ of the Dmrt7 translational start. In the resulting vector, the second and third exons of Dmrt7 are flanked by loxP sites (floxed). The Dmrt7-containing portions of pDZ169 were completely sequenced."}