PMC:1853120 / 23714-24797 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1853120","sourcedb":"PMC","sourceid":"1853120","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1853120","text":"Sertoli cells interact with germ cells during spermatogenesis and the interaction is critical for germ cell maturation [50]. Although we did not detect DMRT7 expression in Sertoli cells by antibody staining, we nevertheless considered the possibility that Sertoli cell defects might contribute to the male-specific germ line failure of Dmrt7 mutants. To characterize Sertoli cell differentiation, we examined expression of the Sertoli cell markers GATA4 (a marker of immature postnatal Sertoli cells) and GATA1 (a mature Sertoli cell marker). The levels of these proteins appeared normal relative to wild-type at P14 and P42 (Figure 6A–6C), as did the androgen receptor (Figure S3; unpublished data). However, the organization of Sertoli cells in Dmrt7 mutant testes was abnormal: in some tubules GATA1-positive Sertoli cell nuclei were displaced from their usual close apposition with the basement membrane (Figure 6C). In such tubules, nuclei of pre-meiotic germ cells and spermatocytes were packed close to the basal membrane and few germ cells were found in the adlumenal 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