PMC:1802744 / 33949-34930 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/1802744","sourcedb":"PMC","sourceid":"1802744","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1802744","text":"Data acquisition and statistical analysis\nImmediately following the washing steps, the fluorescence intensities were measured using a confocal laser scanner (G2505B, Agilent Technologies). After image formatting by Tiff Image Channel Splitter Utility (Agilent Technologies) and grid annotation, a complete set of spots was visually inspected for each slide. Using GenePix Pro (Molecular Devices Corp. USA) flags for artifactual spots were annotated for each spot. Median pixel intensity minus the median local background for both dyes was used to obtain a test over reference intensity ratio. Data normalization was performed per array subgrid using LOWESS curve fitting with a smoothing factor of 0.33 [52,53]. All normalizations, filtering, merging of technical replicates and analyses were performed in the BioArray Software Environment database [32]. To visualize sample-dependent variation of spot intensities, data was uploaded to the TIGR MultiExperiment Viewer (MEV; [34]).","divisions":[{"label":"Title","span":{"begin":0,"end":41}}],"tracks":[]}