PMC:17827 / 55019-58106
Annnotations
2_test
{"project":"2_test","denotations":[{"id":"11178129-8912499-4386941","span":{"begin":40,"end":41},"obj":"8912499"},{"id":"11178129-8003048-4386942","span":{"begin":42,"end":44},"obj":"8003048"},{"id":"11178129-7531793-4386943","span":{"begin":45,"end":47},"obj":"7531793"},{"id":"11178129-1380043-4386944","span":{"begin":48,"end":50},"obj":"1380043"},{"id":"11178129-1411083-4386945","span":{"begin":88,"end":90},"obj":"1411083"},{"id":"11178129-8660103-4386946","span":{"begin":91,"end":93},"obj":"8660103"},{"id":"11178129-1870583-4386947","span":{"begin":94,"end":96},"obj":"1870583"},{"id":"11178129-7531793-4386948","span":{"begin":574,"end":576},"obj":"7531793"},{"id":"11178129-1380043-4386949","span":{"begin":577,"end":579},"obj":"1380043"},{"id":"11178129-8912499-4386950","span":{"begin":690,"end":691},"obj":"8912499"},{"id":"11178129-8003048-4386951","span":{"begin":692,"end":694},"obj":"8003048"},{"id":"11178129-10233853-4386952","span":{"begin":695,"end":697},"obj":"10233853"},{"id":"11178129-8912499-4386953","span":{"begin":712,"end":713},"obj":"8912499"},{"id":"11178129-8003048-4386954","span":{"begin":714,"end":716},"obj":"8003048"},{"id":"11178129-10233853-4386955","span":{"begin":999,"end":1001},"obj":"10233853"},{"id":"11178129-10820282-4386956","span":{"begin":1002,"end":1004},"obj":"10820282"},{"id":"11178129-1411083-4386957","span":{"begin":1155,"end":1157},"obj":"1411083"},{"id":"11178129-8660103-4386958","span":{"begin":1158,"end":1160},"obj":"8660103"},{"id":"11178129-1870583-4386959","span":{"begin":1620,"end":1622},"obj":"1870583"},{"id":"11178129-1411083-4386960","span":{"begin":2592,"end":2594},"obj":"1411083"},{"id":"11178129-2694400-4386961","span":{"begin":3082,"end":3084},"obj":"2694400"}],"text":"Discrepancies\nThe expression of VCAM-1 [1,15,64,65] and Jun and Fos proto-oncogenes [23,25,66,67] showed some in situ / in vitro discrepancies.\nVCAM-1. Surprisingly, only a moderate and variable percentage of RA-SFB (whether conventionally passaged or negatively isolated from primary culture) expressed the adhesion molecule VCAM-1 (Tables 4 and 5). There were also no significant differences between RA-SFB and OA-SFB or normal skin-FB (Table 4). This is in apparent contrast to the enhanced expression of VCAM-1 reported in the lining layer of RA and OA synovial tissue [64,65]; however, it is well compatible with the large variability in VCAM-1 expression observed in vitro (Table 4) [1,15,68] and in situ [1,15]. The significant, positive correlation between VCAM-1 expression in isolated primary-culture RA-SFB and the erythrocyte sedimentation rate in RA patients (ρ=1.00, P = 0.000, n = 4) indicates that the variability may depend on disease activity, as also suggested in recent reports [68,69,70].\nProto-oncogene expression. Negatively isolated RA-SFB expressed c-Fos, c-Jun, and Jun-D (Table 4), in analogy to the features of SFB in RASM [23,25,66]. The expression of c-Jun and Jun-D in the present study could be unequivocally demonstrated by FACS analysis (Table 4), while previous immunohistochemical studies had failed to detect these molecules in situ [23]. Notably, however, the degree of proto-oncogene expression (both percentage of positive cells and MFI) did not significantly differ from that of normal skin-FB (Fig. 6 and Table 4) or, as previously reported, of SFB from traumatic joint injury [67]. This finding supports in situ observations that, at a single-cell level, the degree of proto-oncogene expression in RA, OA, and joint trauma is similar [23], thereby questioning whether proto-oncogene expression in RA reflects per se cell transformation and/or severe metabolic abnormalities.\nNotably, the expression of the proto-oncogenes c-Fos and Jun-D was strikingly increased in conventional fourth-passage RA-SFB as compared with isolated primary-culture RA-SFB (increase of positive cells ≥ 38%; Fig. 7 and Table 5). This increase was limited to RA, since the percentage of cells positive for these molecules was significantly decreased in OA-SFB and numerically decreased in normal skin-FB upon passaging. As a consequence of these reciprocal changes, the percentage of positive cells and/or MFI for c-Fos and Jun-D in conventional fourth-passage RA-SFB was significantly higher than in OA-SFB, under these circumstances confirming previously published data [25].\nFinally, a significant, positive correlation between the percentage of c-fos+ and procollagen I and III+ isolated primary-culture SFB in both RA and OA patients (RA: c-fos/procollagen I, ρ=1.000, P = 0.00, n = 7; c-fos/procollagen III, ρ=0.964, P = 0.00, n = 7) suggests a link between c-fos expression and augmented matrix production by SFB in rheumatic disorders, in line with the known regulation of collagen expression by the activator protein-1 transcription factor (reviewed in [13])."}
Colil
{"project":"Colil","denotations":[{"id":"T123","span":{"begin":712,"end":713},"obj":"8912499"},{"id":"T124","span":{"begin":714,"end":716},"obj":"8003048"},{"id":"T125","span":{"begin":695,"end":697},"obj":"10233853"},{"id":"T131","span":{"begin":3082,"end":3084},"obj":"2694400"},{"id":"T135","span":{"begin":692,"end":694},"obj":"8003048"},{"id":"T137","span":{"begin":40,"end":41},"obj":"8912499"},{"id":"T138","span":{"begin":42,"end":44},"obj":"8003048"},{"id":"T139","span":{"begin":45,"end":47},"obj":"7531793"},{"id":"T140","span":{"begin":48,"end":50},"obj":"1380043"},{"id":"T141","span":{"begin":88,"end":90},"obj":"1411083"},{"id":"T142","span":{"begin":91,"end":93},"obj":"8660103"},{"id":"T143","span":{"begin":94,"end":96},"obj":"1870583"},{"id":"T145","span":{"begin":1155,"end":1157},"obj":"1411083"},{"id":"T146","span":{"begin":1620,"end":1622},"obj":"1870583"},{"id":"T148","span":{"begin":1158,"end":1160},"obj":"8660103"},{"id":"T156","span":{"begin":690,"end":691},"obj":"8912499"},{"id":"T157","span":{"begin":2592,"end":2594},"obj":"1411083"},{"id":"T166","span":{"begin":999,"end":1001},"obj":"10233853"},{"id":"T167","span":{"begin":1002,"end":1004},"obj":"10820282"},{"id":"T169","span":{"begin":574,"end":576},"obj":"7531793"},{"id":"T170","span":{"begin":577,"end":579},"obj":"1380043"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"text":"Discrepancies\nThe expression of VCAM-1 [1,15,64,65] and Jun and Fos proto-oncogenes [23,25,66,67] showed some in situ / in vitro discrepancies.\nVCAM-1. Surprisingly, only a moderate and variable percentage of RA-SFB (whether conventionally passaged or negatively isolated from primary culture) expressed the adhesion molecule VCAM-1 (Tables 4 and 5). There were also no significant differences between RA-SFB and OA-SFB or normal skin-FB (Table 4). This is in apparent contrast to the enhanced expression of VCAM-1 reported in the lining layer of RA and OA synovial tissue [64,65]; however, it is well compatible with the large variability in VCAM-1 expression observed in vitro (Table 4) [1,15,68] and in situ [1,15]. The significant, positive correlation between VCAM-1 expression in isolated primary-culture RA-SFB and the erythrocyte sedimentation rate in RA patients (ρ=1.00, P = 0.000, n = 4) indicates that the variability may depend on disease activity, as also suggested in recent reports [68,69,70].\nProto-oncogene expression. Negatively isolated RA-SFB expressed c-Fos, c-Jun, and Jun-D (Table 4), in analogy to the features of SFB in RASM [23,25,66]. The expression of c-Jun and Jun-D in the present study could be unequivocally demonstrated by FACS analysis (Table 4), while previous immunohistochemical studies had failed to detect these molecules in situ [23]. Notably, however, the degree of proto-oncogene expression (both percentage of positive cells and MFI) did not significantly differ from that of normal skin-FB (Fig. 6 and Table 4) or, as previously reported, of SFB from traumatic joint injury [67]. This finding supports in situ observations that, at a single-cell level, the degree of proto-oncogene expression in RA, OA, and joint trauma is similar [23], thereby questioning whether proto-oncogene expression in RA reflects per se cell transformation and/or severe metabolic abnormalities.\nNotably, the expression of the proto-oncogenes c-Fos and Jun-D was strikingly increased in conventional fourth-passage RA-SFB as compared with isolated primary-culture RA-SFB (increase of positive cells ≥ 38%; Fig. 7 and Table 5). This increase was limited to RA, since the percentage of cells positive for these molecules was significantly decreased in OA-SFB and numerically decreased in normal skin-FB upon passaging. As a consequence of these reciprocal changes, the percentage of positive cells and/or MFI for c-Fos and Jun-D in conventional fourth-passage RA-SFB was significantly higher than in OA-SFB, under these circumstances confirming previously published data [25].\nFinally, a significant, positive correlation between the percentage of c-fos+ and procollagen I and III+ isolated primary-culture SFB in both RA and OA patients (RA: c-fos/procollagen I, ρ=1.000, P = 0.00, n = 7; c-fos/procollagen III, ρ=0.964, P = 0.00, n = 7) suggests a link between c-fos expression and augmented matrix production by SFB in rheumatic disorders, in line with the known regulation of collagen expression by the activator protein-1 transcription factor (reviewed in [13])."}