PMC:17827 / 30054-31050 JSONTXT

{"project":"2_test","denotations":[{"id":"11178129-6366990-4386856","span":{"begin":392,"end":394},"obj":"6366990"},{"id":"11178129-7510485-4386857","span":{"begin":398,"end":400},"obj":"7510485"},{"id":"11178129-9056477-4386858","span":{"begin":891,"end":893},"obj":"9056477"},{"id":"11178129-8752941-4386859","span":{"begin":894,"end":896},"obj":"8752941"},{"id":"11178129-10571119-4386860","span":{"begin":897,"end":899},"obj":"10571119"},{"id":"11178129-10571119-4386861","span":{"begin":992,"end":994},"obj":"10571119"}],"text":"Expression of Thy-1 in RA synovial tissue\nThe mAb AS02 was tested by immunohistochemistry on cryostat sections of RASM to verify the feasibility of this anti-Thy-1 mAb for positive identification/isolation of SFB. The mAb AS02 stained connective tissue cells, while largely sparing the lining layer (see Fig. 3A for details), therefore reproducing the known distribution of FB within the SM [21,23,24]. The mAb AS02 showed no overlap with an anti-CD14 mAb in double-staining experiments, thereby excluding crossreactivity with monocytes/macrophages (Fig. 3C,E). However, the mAb AS02 stained endothelial cells, as demonstrated by double-staining with rabbit Ig against von Willebrand factor (Fig. 3D,F,G). Cultured, non-stimulated HUVEC, in contrast, did not express Thy-1 (data not shown). These results, in line with cytokine induction of Thy-1 expression on endothelial cells in culture [44,45,46], suggest that Thy-1 expression on RASM endothelial cells may reflect ongoing inflammation [46]."}

{"project":"Colil","denotations":[{"id":"T63","span":{"begin":891,"end":893},"obj":"9056477"},{"id":"T64","span":{"begin":894,"end":896},"obj":"8752941"},{"id":"T65","span":{"begin":897,"end":899},"obj":"10571119"},{"id":"T66","span":{"begin":992,"end":994},"obj":"10571119"},{"id":"T67","span":{"begin":392,"end":394},"obj":"6366990"},{"id":"T68","span":{"begin":398,"end":400},"obj":"7510485"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"text":"Expression of Thy-1 in RA synovial tissue\nThe mAb AS02 was tested by immunohistochemistry on cryostat sections of RASM to verify the feasibility of this anti-Thy-1 mAb for positive identification/isolation of SFB. The mAb AS02 stained connective tissue cells, while largely sparing the lining layer (see Fig. 3A for details), therefore reproducing the known distribution of FB within the SM [21,23,24]. The mAb AS02 showed no overlap with an anti-CD14 mAb in double-staining experiments, thereby excluding crossreactivity with monocytes/macrophages (Fig. 3C,E). However, the mAb AS02 stained endothelial cells, as demonstrated by double-staining with rabbit Ig against von Willebrand factor (Fig. 3D,F,G). Cultured, non-stimulated HUVEC, in contrast, did not express Thy-1 (data not shown). These results, in line with cytokine induction of Thy-1 expression on endothelial cells in culture [44,45,46], suggest that Thy-1 expression on RASM endothelial cells may reflect ongoing inflammation [46]."}