PMC:17821 / 17985-19209 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/17821","sourcedb":"PMC","sourceid":"17821","source_url":"http://www.ncbi.nlm.nih.gov/pmc/17821","text":"Induction of arthritis by immune complexes, zymosan, or SCW\nSpecific rabbit anti-lysozyme antisera, made as described elsewhere [35] and made complement-free by heating at 56°C for 30 min, were injected (0.2 ml) intravenously into mice. ICA was then induced by injecting 3 μg of PLL-coupled lysozyme into the right knee joint. The left knee joint was injected with saline solution and used as a control. As an additional control, some mice were given normal rabbit serum instead of specific anti-lysozyme. When PLL-lysozyme was injected into the knee joint without prior administration of specific anti-lysozyme antibodies, no inflammation or cartilage damage developed, in either C57BL/6 or DBA/1 mice.\nZymosan (30 mg) was dissolved in 1 ml saline solution by heating up to 100°C twice and was then sonicated to obtain a homogeneous suspension. Arthritis was induced in other mice by injecting 180 μg zymosan into both left and right knee joints. Fcγ-chain knockout mice and the control strain (C57BL/6) were matched for age and sex.\nSCW arthritis was induced by injecting 25 μg SCWs (rhamnose content), prepared as described elsewhere [37] and dissolved in 6 μl saline, into the right knee joint of C57BL/6 and DBA/1 mice.","divisions":[{"label":"Title","span":{"begin":0,"end":59}}],"tracks":[{"project":"Colil","denotations":[{"id":"T40","span":{"begin":1138,"end":1140},"obj":"3052092"},{"id":"T41","span":{"begin":129,"end":131},"obj":"1605310"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"attributes":[{"subj":"T40","pred":"source","obj":"Colil"},{"subj":"T41","pred":"source","obj":"Colil"}]},{"project":"2_test","denotations":[{"id":"11056679-1605310-4381689","span":{"begin":129,"end":131},"obj":"1605310"},{"id":"11056679-3052092-4381690","span":{"begin":1138,"end":1140},"obj":"3052092"}],"attributes":[{"subj":"11056679-1605310-4381689","pred":"source","obj":"2_test"},{"subj":"11056679-3052092-4381690","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Colil","color":"#98ec93","default":true},{"id":"2_test","color":"#ec93b2"}]}]}}