PMC:17821 / 17452-17983 JSONTXT

{"project":"2_test","denotations":[{"id":"11056679-4111070-4381688","span":{"begin":99,"end":101},"obj":"4111070"}],"text":"Lysozyme coupling to PLL\nLysozyme was coupled to PLL in accordance with the method of Danon et al [36]. As described by those authors, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide was used as an activator and PLL as a nucleophil. Free carboxyl groups of the protein were then coupled to amino groups of PLL. The molecular mass was raised whereas the isoelectric point remained high, as was determined in a 5% polyacrylamide slab gel with 0.8% ampholines (pH gradient 3.5-9.5). The molecular mass appeared to be 74 kD on SDS-PAGE."}

{"project":"Colil","denotations":[{"id":"T39","span":{"begin":99,"end":101},"obj":"4111070"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/"}],"text":"Lysozyme coupling to PLL\nLysozyme was coupled to PLL in accordance with the method of Danon et al [36]. As described by those authors, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide was used as an activator and PLL as a nucleophil. Free carboxyl groups of the protein were then coupled to amino groups of PLL. The molecular mass was raised whereas the isoelectric point remained high, as was determined in a 5% polyacrylamide slab gel with 0.8% ampholines (pH gradient 3.5-9.5). The molecular mass appeared to be 74 kD on SDS-PAGE."}