PMC:1702488 / 8541-9186 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1702488","sourcedb":"PMC","sourceid":"1702488","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1702488","text":"Real-time PCR\nTotal RNA was extracted using the TRIzol reagent (Molecular Research Center, Inc.) from NS20Y cells. DNase I-treated RNA (2 μg) was subjected to RT (Roche) using oligo(dT) primer. One-fortieth of this reaction was used for real-time PCR analysis using SYBR Green I dye chemistry. PCR product accumulation was monitored using a iCycler iQ Real-Time detection system (Bio-Rad). The mean cycle threshold value (Ct) from triplicate samples was used to calculate gene expression level. PCR products were normalized to levels of β-actin. Relative gene expression levels were determined as described in User's resource guide from Bio-Rad.","divisions":[{"label":"Title","span":{"begin":0,"end":13}}],"tracks":[]}