PMC:1635039 / 32360-32767 JSONTXT

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    craft-sa-dev

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(E10.0 – E13.0) were dissected and placed in ice cold PBS (n≥3 per genotype in each time point). 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    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T16947","span":{"begin":0,"end":7},"obj":"UBERON:0000922"},{"id":"T16948","span":{"begin":75,"end":83},"obj":"SO_EXT:genotype_or_entity_with_genotype"},{"id":"T16949","span":{"begin":163,"end":166},"obj":"CHEBI_EXT:37958"},{"id":"T16950","span":{"begin":187,"end":197},"obj":"CHEBI_GO_EXT:biological_process_or_quality_or_role"},{"id":"T16951","span":{"begin":228,"end":238},"obj":"UBERON:0002082"},{"id":"T16952","span":{"begin":249,"end":252},"obj":"CHEBI_EXT:37958"},{"id":"T16953","span":{"begin":279,"end":286},"obj":"UBERON:0000922"},{"id":"T16954","span":{"begin":309,"end":317},"obj":"CHEBI_CHMO_EXT:buffer_process"},{"id":"T16955","span":{"begin":318,"end":326},"obj":"CHEBI:16842"},{"id":"T16956","span":{"begin":367,"end":381},"obj":"CHEBI:41237"},{"id":"T16957","span":{"begin":383,"end":397},"obj":"CHEBI:17987"}],"text":"Embryos (E10.0 – E13.0) were dissected and placed in ice cold PBS (n≥3 per genotype in each time point). Using a pulled glass pipette, India ink or Yellow casting dye (Connecticut Valley Biological Supply) was injected into the ventricles until ink/dye penetrated small vessels. Embryos were postfixed in 10% buffered formalin for 12 hours, dehydrated and cleared in benzylbenzoate: benzyl alcohol (2:1v/v)."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T16938","span":{"begin":0,"end":7},"obj":"UBERON:0000922"},{"id":"T16939","span":{"begin":163,"end":166},"obj":"CHEBI:37958"},{"id":"T16940","span":{"begin":228,"end":238},"obj":"UBERON:0002082"},{"id":"T16941","span":{"begin":249,"end":252},"obj":"CHEBI:37958"},{"id":"T16942","span":{"begin":279,"end":286},"obj":"UBERON:0000922"},{"id":"T16943","span":{"begin":318,"end":326},"obj":"CHEBI:16842"},{"id":"T16944","span":{"begin":367,"end":381},"obj":"CHEBI:41237"},{"id":"T16945","span":{"begin":383,"end":397},"obj":"CHEBI:17987"}],"text":"Embryos (E10.0 – E13.0) were dissected and placed in ice cold PBS (n≥3 per genotype in each time point). Using a pulled glass pipette, India ink or Yellow casting dye (Connecticut Valley Biological Supply) was injected into the ventricles until ink/dye penetrated small vessels. Embryos were postfixed in 10% buffered formalin for 12 hours, dehydrated and cleared in benzylbenzoate: benzyl alcohol (2:1v/v)."}