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PMC:1635039 / 31436-32327 JSONTXT

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Id Subject Object Predicate Lexical cue
T16578 0-7 NNS denotes Embryos
T16579 19-24 VBN denotes fixed
T16580 8-9 -LRB- denotes (
T16581 9-12 NN denotes E17
T16582 12-13 -RRB- denotes )
T16583 14-18 VBD denotes were
T16584 25-29 IN denotes with
T16585 30-31 CD denotes 4
T16586 31-32 NN denotes %
T16587 33-49 NN denotes paraformaldehyhe
T16588 50-53 IN denotes for
T16589 54-56 CD denotes 14
T16590 57-62 NNS denotes hours
T16591 62-64 , denotes ,
T16592 64-74 VBN denotes dehydrated
T16593 75-78 CC denotes and
T16594 79-87 VBN denotes embedded
T16595 88-90 IN denotes in
T16596 91-99 NN denotes paraffin
T16597 100-103 NN denotes wax
T16598 103-104 . denotes .
T16599 104-187 sentence denotes Sections (7–8 um) were stained with Hematoxylin and Eosin (n≥3 for each genotype).
T16600 105-113 NNS denotes Sections
T16601 128-135 VBN denotes stained
T16602 114-115 -LRB- denotes (
T16603 119-121 NN denotes um
T16604 115-116 CD denotes 7
T16605 117-118 CD denotes 8
T16606 116-117 SYM denotes –
T16607 121-122 -RRB- denotes )
T16608 123-127 VBD denotes were
T16609 136-140 IN denotes with
T16610 141-152 NN denotes Hematoxylin
T16611 153-156 CC denotes and
T16612 157-162 NN denotes Eosin
T16613 163-164 -LRB- denotes (
T16614 166-167 CD denotes 3
T16615 164-165 NN denotes n
T16616 165-166 SYM denotes ≥
T16617 168-171 IN denotes for
T16618 172-176 DT denotes each
T16619 177-185 NN denotes genotype
T16620 185-186 -RRB- denotes )
T16621 186-187 . denotes .
T16622 187-286 sentence denotes For lineage tracing analyses, embryos were stained for β-galactosidase activity as described [43].
T16623 188-191 IN denotes For
T16624 231-238 VBN denotes stained
T16625 192-199 NN denotes lineage
T16626 200-207 NN denotes tracing
T16627 208-216 NNS denotes analyses
T16628 216-218 , denotes ,
T16629 218-225 NNS denotes embryos
T16630 226-230 VBD denotes were
T16631 239-242 IN denotes for
T16632 243-244 NN denotes β
T16633 245-258 NN denotes galactosidase
T16634 244-245 HYPH denotes -
T16635 259-267 NN denotes activity
T16636 268-270 IN denotes as
T16637 271-280 VBN denotes described
T16638 281-282 -LRB- denotes [
T16639 282-284 CD denotes 43
T16640 284-285 -RRB- denotes ]
T16641 285-286 . denotes .
T16642 286-519 sentence denotes Briefly, the specimens (E11.0 – E11.5) were fixed in 4% paraformaldehyde for 30 minutes at room temperature, washed 3 times for 10 minutes in the detergent wash and developed for 2–12 hours in X-gal solution (n≥3 for each genotype).
T16643 287-294 RB denotes Briefly
T16644 331-336 VBN denotes fixed
T16645 294-296 , denotes ,
T16646 296-299 DT denotes the
T16647 300-309 NNS denotes specimens
T16648 310-311 -LRB- denotes (
T16649 311-316 NN denotes E11.0
T16650 317-318 SYM denotes –
T16651 319-324 NN denotes E11.5
T16652 324-325 -RRB- denotes )
T16653 326-330 VBD denotes were
T16654 337-339 IN denotes in
T16655 340-341 CD denotes 4
T16656 341-342 NN denotes %
T16657 343-359 NN denotes paraformaldehyde
T16658 360-363 IN denotes for
T16659 364-366 CD denotes 30
T16660 367-374 NNS denotes minutes
T16661 375-377 IN denotes at
T16662 378-382 NN denotes room
T16663 383-394 NN denotes temperature
T16664 394-396 , denotes ,
T16665 396-402 VBN denotes washed
T16666 403-404 CD denotes 3
T16667 405-410 NNS denotes times
T16668 411-414 IN denotes for
T16669 415-417 CD denotes 10
T16670 418-425 NNS denotes minutes
T16671 426-428 IN denotes in
T16672 429-432 DT denotes the
T16673 443-447 NN denotes wash
T16674 433-442 NN denotes detergent
T16675 448-451 CC denotes and
T16676 452-461 VBN denotes developed
T16677 462-465 IN denotes for
T16678 466-467 CD denotes 2
T16679 468-470 CD denotes 12
T16680 467-468 SYM denotes –
T16681 471-476 NNS denotes hours
T16682 477-479 IN denotes in
T16683 480-481 NN denotes X
T16684 482-485 NN denotes gal
T16685 481-482 HYPH denotes -
T16686 486-494 NN denotes solution
T16687 495-496 -LRB- denotes (
T16688 498-499 CD denotes 3
T16689 496-497 NN denotes n
T16690 497-498 SYM denotes ≥
T16691 500-503 IN denotes for
T16692 504-508 DT denotes each
T16693 509-517 NN denotes genotype
T16694 517-518 -RRB- denotes )
T16695 518-519 . denotes .
T16696 519-743 sentence denotes For immunohistochemistry, fixed sections from tissues harvested at E10.5 – E11.5 were stained with monoclonal α-smooth muscle actin (DAKO), cleaved caspase-3 (Cell Signaling) or phophohistone-H3 (Cell Signaling) antibodies.
T16697 520-523 IN denotes For
T16698 606-613 VBN denotes stained
T16699 524-544 NN denotes immunohistochemistry
T16700 544-546 , denotes ,
T16701 546-551 VBN denotes fixed
T16702 552-560 NNS denotes sections
T16703 561-565 IN denotes from
T16704 566-573 NNS denotes tissues
T16705 574-583 VBN denotes harvested
T16706 584-586 IN denotes at
T16707 587-592 NN denotes E10.5
T16708 593-594 SYM denotes –
T16709 595-600 NN denotes E11.5
T16710 601-605 VBD denotes were
T16711 614-618 IN denotes with
T16712 619-629 JJ denotes monoclonal
T16713 646-651 NN denotes actin
T16714 630-631 NN denotes α
T16715 639-645 NN denotes muscle
T16716 631-632 HYPH denotes -
T16717 632-638 JJ denotes smooth
T16718 732-742 NNS denotes antibodies
T16719 652-653 -LRB- denotes (
T16720 653-657 NN denotes DAKO
T16721 657-658 -RRB- denotes )
T16722 658-660 , denotes ,
T16723 660-667 VBN denotes cleaved
T16724 668-675 NN denotes caspase
T16725 675-676 HYPH denotes -
T16726 676-677 CD denotes 3
T16727 678-679 -LRB- denotes (
T16728 684-693 NNP denotes Signaling
T16729 679-683 NNP denotes Cell
T16730 693-694 -RRB- denotes )
T16731 695-697 CC denotes or
T16732 698-711 NN denotes phophohistone
T16733 712-714 NN denotes H3
T16734 711-712 HYPH denotes -
T16735 715-716 -LRB- denotes (
T16736 721-730 NNP denotes Signaling
T16737 716-720 NNP denotes Cell
T16738 730-731 -RRB- denotes )
T16739 742-743 . denotes .
T16740 743-826 sentence denotes TUNEL assays were performed using the DeadEnd fluorometric TUNEL system (Promega).
T16741 744-749 NN denotes TUNEL
T16742 750-756 NNS denotes assays
T16743 762-771 VBN denotes performed
T16744 757-761 VBD denotes were
T16745 772-777 VBG denotes using
T16746 778-781 DT denotes the
T16747 809-815 NN denotes system
T16748 782-789 NNP denotes DeadEnd
T16749 803-808 NN denotes TUNEL
T16750 790-802 JJ denotes fluorometric
T16751 816-817 -LRB- denotes (
T16752 817-824 NNP denotes Promega
T16753 824-825 -RRB- denotes )
T16754 825-826 . denotes .
T16755 826-891 sentence denotes In each assay 3 or more embryos were analyzed for each genotype.
T16756 827-829 IN denotes In
T16757 864-872 VBN denotes analyzed
T16758 830-834 DT denotes each
T16759 835-840 NN denotes assay
T16760 841-842 CD denotes 3
T16761 851-858 NNS denotes embryos
T16762 843-845 CC denotes or
T16763 846-850 JJR denotes more
T16764 859-863 VBD denotes were
T16765 873-876 IN denotes for
T16766 877-881 DT denotes each
T16767 882-890 NN denotes genotype
T16768 890-891 . denotes .
R4985 T16578 T16579 nsubjpass Embryos,fixed
R4986 T16580 T16581 punct (,E17
R4987 T16581 T16578 parataxis E17,Embryos
R4988 T16582 T16581 punct ),E17
R4989 T16583 T16579 auxpass were,fixed
R4990 T16584 T16579 prep with,fixed
R4991 T16585 T16586 nummod 4,%
R4992 T16586 T16587 compound %,paraformaldehyhe
R4993 T16587 T16584 pobj paraformaldehyhe,with
R4994 T16588 T16579 prep for,fixed
R4995 T16589 T16590 nummod 14,hours
R4996 T16590 T16588 pobj hours,for
R4997 T16591 T16579 punct ", ",fixed
R4998 T16592 T16579 conj dehydrated,fixed
R4999 T16593 T16592 cc and,dehydrated
R5000 T16594 T16592 conj embedded,dehydrated
R5001 T16595 T16594 prep in,embedded
R5002 T16596 T16597 compound paraffin,wax
R5003 T16597 T16595 pobj wax,in
R5004 T16598 T16579 punct .,fixed
R5005 T16600 T16601 nsubjpass Sections,stained
R5006 T16602 T16603 punct (,um
R5007 T16603 T16600 parataxis um,Sections
R5008 T16604 T16605 quantmod 7,8
R5009 T16605 T16603 nummod 8,um
R5010 T16606 T16605 punct –,8
R5011 T16607 T16603 punct ),um
R5012 T16608 T16601 auxpass were,stained
R5013 T16609 T16601 prep with,stained
R5014 T16610 T16609 pobj Hematoxylin,with
R5015 T16611 T16610 cc and,Hematoxylin
R5016 T16612 T16610 conj Eosin,Hematoxylin
R5017 T16613 T16614 punct (,3
R5018 T16614 T16601 parataxis 3,stained
R5019 T16615 T16614 nsubj n,3
R5020 T16616 T16614 punct ≥,3
R5021 T16617 T16614 prep for,3
R5022 T16618 T16619 det each,genotype
R5023 T16619 T16617 pobj genotype,for
R5024 T16620 T16614 punct ),3
R5025 T16621 T16601 punct .,stained
R5026 T16623 T16624 prep For,stained
R5027 T16625 T16626 compound lineage,tracing
R5028 T16626 T16627 compound tracing,analyses
R5029 T16627 T16623 pobj analyses,For
R5030 T16628 T16624 punct ", ",stained
R5031 T16629 T16624 nsubjpass embryos,stained
R5032 T16630 T16624 auxpass were,stained
R5033 T16631 T16624 prep for,stained
R5034 T16632 T16633 compound β,galactosidase
R5035 T16633 T16635 compound galactosidase,activity
R5036 T16634 T16633 punct -,galactosidase
R5037 T16635 T16631 pobj activity,for
R5038 T16636 T16637 mark as,described
R5039 T16637 T16624 advcl described,stained
R5040 T16638 T16639 punct [,43
R5041 T16639 T16624 parataxis 43,stained
R5042 T16640 T16639 punct ],43
R5043 T16641 T16624 punct .,stained
R5044 T16643 T16644 advmod Briefly,fixed
R5045 T16645 T16644 punct ", ",fixed
R5046 T16646 T16647 det the,specimens
R5047 T16647 T16644 nsubjpass specimens,fixed
R5048 T16648 T16649 punct (,E11.0
R5049 T16649 T16647 parataxis E11.0,specimens
R5050 T16650 T16651 punct –,E11.5
R5051 T16651 T16649 prep E11.5,E11.0
R5052 T16652 T16649 punct ),E11.0
R5053 T16653 T16644 auxpass were,fixed
R5054 T16654 T16644 prep in,fixed
R5055 T16655 T16656 nummod 4,%
R5056 T16656 T16657 compound %,paraformaldehyde
R5057 T16657 T16654 pobj paraformaldehyde,in
R5058 T16658 T16644 prep for,fixed
R5059 T16659 T16660 nummod 30,minutes
R5060 T16660 T16658 pobj minutes,for
R5061 T16661 T16644 prep at,fixed
R5062 T16662 T16663 compound room,temperature
R5063 T16663 T16661 pobj temperature,at
R5064 T16664 T16644 punct ", ",fixed
R5065 T16665 T16644 conj washed,fixed
R5066 T16666 T16667 nummod 3,times
R5067 T16667 T16665 npadvmod times,washed
R5068 T16668 T16665 prep for,washed
R5069 T16669 T16670 nummod 10,minutes
R5070 T16670 T16668 pobj minutes,for
R5071 T16671 T16665 prep in,washed
R5072 T16672 T16673 det the,wash
R5073 T16673 T16671 pobj wash,in
R5074 T16674 T16673 compound detergent,wash
R5075 T16675 T16665 cc and,washed
R5076 T16676 T16665 conj developed,washed
R5077 T16677 T16676 prep for,developed
R5078 T16678 T16679 quantmod 2,12
R5079 T16679 T16681 nummod 12,hours
R5080 T16680 T16679 punct –,12
R5081 T16681 T16677 pobj hours,for
R5082 T16682 T16676 prep in,developed
R5083 T16683 T16684 compound X,gal
R5084 T16684 T16686 compound gal,solution
R5085 T16685 T16684 punct -,gal
R5086 T16686 T16682 pobj solution,in
R5087 T16687 T16688 punct (,3
R5088 T16688 T16676 parataxis 3,developed
R5089 T16689 T16688 nsubj n,3
R5090 T16690 T16688 punct ≥,3
R5091 T16691 T16688 prep for,3
R5092 T16692 T16693 det each,genotype
R5093 T16693 T16691 pobj genotype,for
R5094 T16694 T16688 punct ),3
R5095 T16695 T16644 punct .,fixed
R5096 T16697 T16698 prep For,stained
R5097 T16699 T16697 pobj immunohistochemistry,For
R5098 T16700 T16698 punct ", ",stained
R5099 T16701 T16702 amod fixed,sections
R5100 T16702 T16698 nsubjpass sections,stained
R5101 T16703 T16702 prep from,sections
R5102 T16704 T16703 pobj tissues,from
R5103 T16705 T16704 acl harvested,tissues
R5104 T16706 T16705 prep at,harvested
R5105 T16707 T16706 pobj E10.5,at
R5106 T16708 T16709 punct –,E11.5
R5107 T16709 T16707 prep E11.5,E10.5
R5108 T16710 T16698 auxpass were,stained
R5109 T16711 T16698 prep with,stained
R5110 T16712 T16713 amod monoclonal,actin
R5111 T16713 T16718 nmod actin,antibodies
R5112 T16714 T16715 nmod α,muscle
R5113 T16715 T16713 nmod muscle,actin
R5114 T16716 T16715 punct -,muscle
R5115 T16717 T16715 amod smooth,muscle
R5116 T16718 T16711 pobj antibodies,with
R5117 T16719 T16720 punct (,DAKO
R5118 T16720 T16713 parataxis DAKO,actin
R5119 T16721 T16720 punct ),DAKO
R5120 T16722 T16713 punct ", ",actin
R5121 T16723 T16724 amod cleaved,caspase
R5122 T16724 T16713 conj caspase,actin
R5123 T16725 T16724 punct -,caspase
R5124 T16726 T16724 nummod 3,caspase
R5125 T16727 T16728 punct (,Signaling
R5126 T16728 T16724 parataxis Signaling,caspase
R5127 T16729 T16728 compound Cell,Signaling
R5128 T16730 T16728 punct ),Signaling
R5129 T16731 T16724 cc or,caspase
R5130 T16732 T16733 compound phophohistone,H3
R5131 T16733 T16724 conj H3,caspase
R5132 T16734 T16733 punct -,H3
R5133 T16735 T16736 punct (,Signaling
R5134 T16736 T16733 parataxis Signaling,H3
R5135 T16737 T16736 compound Cell,Signaling
R5136 T16738 T16736 punct ),Signaling
R5137 T16739 T16698 punct .,stained
R5138 T16741 T16742 compound TUNEL,assays
R5139 T16742 T16743 nsubjpass assays,performed
R5140 T16744 T16743 auxpass were,performed
R5141 T16745 T16743 advcl using,performed
R5142 T16746 T16747 det the,system
R5143 T16747 T16745 dobj system,using
R5144 T16748 T16749 nmod DeadEnd,TUNEL
R5145 T16749 T16747 compound TUNEL,system
R5146 T16750 T16749 amod fluorometric,TUNEL
R5147 T16751 T16752 punct (,Promega
R5148 T16752 T16745 parataxis Promega,using
R5149 T16753 T16752 punct ),Promega
R5150 T16754 T16743 punct .,performed
R5151 T16756 T16757 prep In,analyzed
R5152 T16758 T16759 det each,assay
R5153 T16759 T16756 pobj assay,In
R5154 T16760 T16761 nummod 3,embryos
R5155 T16761 T16757 nsubjpass embryos,analyzed
R5156 T16762 T16760 cc or,3
R5157 T16763 T16760 conj more,3
R5158 T16764 T16757 auxpass were,analyzed
R5159 T16765 T16757 prep for,analyzed
R5160 T16766 T16767 det each,genotype
R5161 T16767 T16765 pobj genotype,for
R5162 T16768 T16757 punct .,analyzed

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T16548 0-7 UBERON:0000922 denotes Embryos
T16549 33-49 CHEBI_EXT:paraformaldehye denotes paraformaldehyhe
T16550 100-103 CHEBI:73702 denotes wax
T16551 141-152 CHEBI:51686 denotes Hematoxylin
T16552 157-162 CHEBI_EXT:eosin denotes Eosin
T16553 177-185 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T16554 218-225 UBERON:0000922 denotes embryos
T16555 243-258 GO_EXT:0004565 denotes β-galactosidase
T16556 343-359 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T16557 433-442 CHEBI_EXT:27780 denotes detergent
T16558 480-485 CHEBI:75055 denotes X-gal
T16559 486-494 CHEBI:75958 denotes solution
T16560 509-517 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T16561 566-573 UBERON:0000479 denotes tissues
T16562 630-651 PR_EXT:000003675 denotes α-smooth muscle actin
T16563 632-645 UBERON:0001135 denotes smooth muscle
T16564 660-667 MOP:0000780 denotes cleaved
T16565 660-677 PR_EXT:000018762 denotes cleaved caspase-3
T16566 679-683 CL_GO_EXT:cell denotes Cell
T16567 684-693 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T16568 698-711 CHEBI_EXT:phosphohistone denotes phophohistone
T16569 712-714 PR_EXT:000027594 denotes H3
T16570 716-720 CL_GO_EXT:cell denotes Cell
T16571 721-730 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T16572 732-742 GO:0042571 denotes antibodies
T16573 851-858 UBERON:0000922 denotes embryos
T16574 882-890 SO_EXT:genotype_or_entity_with_genotype denotes genotype

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T16533 0-7 UBERON:0000922 denotes Embryos
T16534 100-103 CHEBI:73702 denotes wax
T16535 141-152 CHEBI:51686 denotes Hematoxylin
T16536 218-225 UBERON:0000922 denotes embryos
T16537 433-442 CHEBI:27780 denotes detergent
T16538 480-485 CHEBI:75055 denotes X-gal
T16539 486-494 CHEBI:75958 denotes solution
T16540 566-573 UBERON:0000479 denotes tissues
T16541 630-651 PR:000003675 denotes α-smooth muscle actin
T16542 632-645 UBERON:0001135 denotes smooth muscle
T16543 660-667 MOP:0000780 denotes cleaved
T16544 660-677 PR:000018762 denotes cleaved caspase-3
T16545 712-714 PR:000027594 denotes H3
T16546 732-742 GO:0042571 denotes antibodies
T16547 851-858 UBERON:0000922 denotes embryos