PMC:1635039 / 18403-19779 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T9880","span":{"begin":135,"end":142},"obj":"NNS"},{"id":"T9852","span":{"begin":0,"end":2},"obj":"IN"},{"id":"T9853","span":{"begin":3,"end":12},"obj":"VBN"},{"id":"T9854","span":{"begin":42,"end":51},"obj":"VBD"},{"id":"T9855","span":{"begin":13,"end":18},"obj":"RB"},{"id":"T9856","span":{"begin":18,"end":20},"obj":","},{"id":"T9857","span":{"begin":20,"end":24},"obj":"NN"},{"id":"T9858","span":{"begin":30,"end":33},"obj":"NN"},{"id":"T9859","span":{"begin":24,"end":25},"obj":"HYPH"},{"id":"T9860","span":{"begin":25,"end":29},"obj":"NN"},{"id":"T9861","span":{"begin":29,"end":30},"obj":"HYPH"},{"id":"T9862","span":{"begin":34,"end":41},"obj":"NNS"},{"id":"T9863","span":{"begin":52,"end":54},"obj":"DT"},{"id":"T9864","span":{"begin":66,"end":72},"obj":"NN"},{"id":"T9865","span":{"begin":55,"end":65},"obj":"JJ"},{"id":"T9866","span":{"begin":73,"end":75},"obj":"IN"},{"id":"T9867","span":{"begin":76,"end":78},"obj":"NN"},{"id":"T9868","span":{"begin":79,"end":85},"obj":"JJ"},{"id":"T9869","span":{"begin":78,"end":79},"obj":"HYPH"},{"id":"T9870","span":{"begin":86,"end":92},"obj":"NN"},{"id":"T9871","span":{"begin":93,"end":95},"obj":"IN"},{"id":"T9872","span":{"begin":96,"end":99},"obj":"DT"},{"id":"T9873","span":{"begin":100,"end":104},"obj":"NN"},{"id":"T9874","span":{"begin":105,"end":107},"obj":"IN"},{"id":"T9875","span":{"begin":108,"end":111},"obj":"DT"},{"id":"T9876","span":{"begin":119,"end":122},"obj":"NN"},{"id":"T9877","span":{"begin":112,"end":118},"obj":"JJ"},{"id":"T9878","span":{"begin":123,"end":130},"obj":"IN"},{"id":"T9879","span":{"begin":131,"end":134},"obj":"DT"},{"id":"T9881","span":{"begin":143,"end":145},"obj":"IN"},{"id":"T9882","span":{"begin":146,"end":149},"obj":"JJ"},{"id":"T9883","span":{"begin":158,"end":162},"obj":"NNS"},{"id":"T9884","span":{"begin":150,"end":153},"obj":"CC"},{"id":"T9885","span":{"begin":154,"end":157},"obj":"JJ"},{"id":"T9886","span":{"begin":162,"end":163},"obj":"."},{"id":"T9887","span":{"begin":163,"end":322},"obj":"sentence"},{"id":"T9888","span":{"begin":164,"end":166},"obj":"TO"},{"id":"T9889","span":{"begin":167,"end":174},"obj":"VB"},{"id":"T9890","span":{"begin":279,"end":283},"obj":"VBD"},{"id":"T9891","span":{"begin":175,"end":182},"obj":"IN"},{"id":"T9892","span":{"begin":198,"end":206},"obj":"VBD"},{"id":"T9893","span":{"begin":183,"end":187},"obj":"DT"},{"id":"T9894","span":{"begin":188,"end":197},"obj":"NN"},{"id":"T9895","span":{"begin":207,"end":213},"obj":"CC"},{"id":"T9896","span":{"begin":214,"end":218},"obj":"IN"},{"id":"T9897","span":{"begin":219,"end":228},"obj":"JJ"},{"id":"T9898","span":{"begin":234,"end":247},"obj":"NN"},{"id":"T9899","span":{"begin":229,"end":233},"obj":"NN"},{"id":"T9900","span":{"begin":248,"end":250},"obj":"CC"},{"id":"T9901","span":{"begin":251,"end":264},"obj":"JJ"},{"id":"T9902","span":{"begin":265,"end":274},"obj":"NN"},{"id":"T9903","span":{"begin":274,"end":276},"obj":","},{"id":"T9904","span":{"begin":276,"end":278},"obj":"PRP"},{"id":"T9905","span":{"begin":284,"end":288},"obj":"NN"},{"id":"T9906","span":{"begin":299,"end":307},"obj":"NN"},{"id":"T9907","span":{"begin":289,"end":292},"obj":"CC"},{"id":"T9908","span":{"begin":293,"end":298},"obj":"NN"},{"id":"T9909","span":{"begin":307,"end":309},"obj":","},{"id":"T9910","span":{"begin":309,"end":321},"obj":"RB"},{"id":"T9911","span":{"begin":321,"end":322},"obj":"."},{"id":"T9912","span":{"begin":322,"end":568},"obj":"sentence"},{"id":"T9913","span":{"begin":323,"end":328},"obj":"IN"},{"id":"T9914","span":{"begin":356,"end":364},"obj":"VBN"},{"id":"T9915","span":{"begin":329,"end":333},"obj":"NN"},{"id":"T9916","span":{"begin":334,"end":347},"obj":"NN"},{"id":"T9917","span":{"begin":348,"end":351},"obj":"VBD"},{"id":"T9918","span":{"begin":352,"end":355},"obj":"RB"},{"id":"T9919","span":{"begin":408,"end":414},"obj":"VB"},{"id":"T9920","span":{"begin":365,"end":367},"obj":"IN"},{"id":"T9921","span":{"begin":368,"end":372},"obj":"NN"},{"id":"T9922","span":{"begin":373,"end":380},"obj":"NNS"},{"id":"T9923","span":{"begin":381,"end":382},"obj":"-LRB-"},{"id":"T9924","span":{"begin":391,"end":396},"obj":"VBN"},{"id":"T9925","span":{"begin":382,"end":386},"obj":"NNS"},{"id":"T9926","span":{"begin":387,"end":390},"obj":"RB"},{"id":"T9927","span":{"begin":396,"end":397},"obj":"-RRB-"},{"id":"T9928","span":{"begin":397,"end":399},"obj":","},{"id":"T9929","span":{"begin":399,"end":401},"obj":"PRP"},{"id":"T9930","span":{"begin":402,"end":407},"obj":"MD"},{"id":"T9931","span":{"begin":415,"end":416},"obj":"DT"},{"id":"T9932","span":{"begin":426,"end":434},"obj":"NN"},{"id":"T9933","span":{"begin":417,"end":425},"obj":"JJ"},{"id":"T9934","span":{"begin":435,"end":437},"obj":"IN"},{"id":"T9935","span":{"begin":438,"end":441},"obj":"DT"},{"id":"T9936","span":{"begin":442,"end":448},"obj":"NN"},{"id":"T9937","span":{"begin":449,"end":451},"obj":"IN"},{"id":"T9938","span":{"begin":452,"end":457},"obj":"NN"},{"id":"T9939","span":{"begin":458,"end":466},"obj":"JJ"},{"id":"T9940","span":{"begin":467,"end":472},"obj":"NNS"},{"id":"T9941","span":{"begin":473,"end":475},"obj":"IN"},{"id":"T9942","span":{"begin":476,"end":483},"obj":"NNS"},{"id":"T9943","span":{"begin":484,"end":495},"obj":"VBG"},{"id":"T9944","span":{"begin":496,"end":499},"obj":"DT"},{"id":"T9945","span":{"begin":507,"end":510},"obj":"NN"},{"id":"T9946","span":{"begin":500,"end":506},"obj":"JJ"},{"id":"T9947","span":{"begin":511,"end":520},"obj":"VBG"},{"id":"T9948","span":{"begin":521,"end":524},"obj":"DT"},{"id":"T9949","span":{"begin":525,"end":529},"obj":"NN"},{"id":"T9950","span":{"begin":530,"end":535},"obj":"WRB"},{"id":"T9951","span":{"begin":550,"end":555},"obj":"VBZ"},{"id":"T9952","span":{"begin":536,"end":539},"obj":"DT"},{"id":"T9953","span":{"begin":543,"end":549},"obj":"NN"},{"id":"T9954","span":{"begin":540,"end":542},"obj":"NN"},{"id":"T9955","span":{"begin":542,"end":543},"obj":"HYPH"},{"id":"T9956","span":{"begin":556,"end":557},"obj":"-LRB-"},{"id":"T9957","span":{"begin":562,"end":564},"obj":"NN"},{"id":"T9958","span":{"begin":557,"end":561},"obj":"NN"},{"id":"T9959","span":{"begin":564,"end":565},"obj":"SYM"},{"id":"T9960","span":{"begin":565,"end":566},"obj":"NN"},{"id":"T9961","span":{"begin":566,"end":567},"obj":"-RRB-"},{"id":"T9962","span":{"begin":567,"end":568},"obj":"."},{"id":"T9963","span":{"begin":568,"end":728},"obj":"sentence"},{"id":"T9964","span":{"begin":569,"end":573},"obj":"JJ"},{"id":"T9965","span":{"begin":574,"end":582},"obj":"NN"},{"id":"T9966","span":{"begin":617,"end":629},"obj":"VBD"},{"id":"T9967","span":{"begin":583,"end":586},"obj":"IN"},{"id":"T9968","span":{"begin":587,"end":591},"obj":"NN"},{"id":"T9969","span":{"begin":602,"end":610},"obj":"JJ"},{"id":"T9970","span":{"begin":592,"end":595},"obj":"CC"},{"id":"T9971","span":{"begin":596,"end":601},"obj":"NN"},{"id":"T9972","span":{"begin":611,"end":616},"obj":"NNS"},{"id":"T9973","span":{"begin":688,"end":691},"obj":"VBD"},{"id":"T9974","span":{"begin":630,"end":634},"obj":"IN"},{"id":"T9975","span":{"begin":647,"end":651},"obj":"VBD"},{"id":"T9976","span":{"begin":635,"end":640},"obj":"DT"},{"id":"T9977","span":{"begin":641,"end":646},"obj":"NNS"},{"id":"T9978","span":{"begin":652,"end":665},"obj":"JJ"},{"id":"T9979","span":{"begin":666,"end":671},"obj":"NNS"},{"id":"T9980","span":{"begin":671,"end":672},"obj":":"},{"id":"T9981","span":{"begin":673,"end":677},"obj":"DT"},{"id":"T9982","span":{"begin":678,"end":687},"obj":"NN"},{"id":"T9983","span":{"begin":692,"end":699},"obj":"RB"},{"id":"T9984","span":{"begin":700,"end":707},"obj":"RB"},{"id":"T9985","span":{"begin":708,"end":718},"obj":"JJ"},{"id":"T9986","span":{"begin":719,"end":721},"obj":"IN"},{"id":"T9987","span":{"begin":722,"end":727},"obj":"NN"},{"id":"T9988","span":{"begin":727,"end":728},"obj":"."},{"id":"T9989","span":{"begin":728,"end":846},"obj":"sentence"},{"id":"T9990","span":{"begin":729,"end":734},"obj":"DT"},{"id":"T9991","span":{"begin":735,"end":742},"obj":"NNS"},{"id":"T9992","span":{"begin":748,"end":757},"obj":"VBN"},{"id":"T9993","span":{"begin":743,"end":747},"obj":"VBD"},{"id":"T9994","span":{"begin":758,"end":760},"obj":"IN"},{"id":"T9995","span":{"begin":761,"end":766},"obj":"VBG"},{"id":"T9996","span":{"begin":767,"end":781},"obj":"NN"},{"id":"T9997","span":{"begin":782,"end":785},"obj":"IN"},{"id":"T9998","span":{"begin":786,"end":793},"obj":"VBN"},{"id":"T9999","span":{"begin":794,"end":801},"obj":"NN"},{"id":"T10000","span":{"begin":801,"end":802},"obj":"HYPH"},{"id":"T10001","span":{"begin":802,"end":803},"obj":"CD"},{"id":"T10002","span":{"begin":803,"end":805},"obj":","},{"id":"T10003","span":{"begin":805,"end":812},"obj":"DT"},{"id":"T10004","span":{"begin":813,"end":819},"obj":"NN"},{"id":"T10005","span":{"begin":820,"end":823},"obj":"IN"},{"id":"T10006","span":{"begin":824,"end":833},"obj":"NN"},{"id":"T10007","span":{"begin":834,"end":835},"obj":"-LRB-"},{"id":"T10008","span":{"begin":843,"end":844},"obj":"NN"},{"id":"T10009","span":{"begin":835,"end":839},"obj":"NNP"},{"id":"T10010","span":{"begin":840,"end":842},"obj":"NN"},{"id":"T10011","span":{"begin":842,"end":843},"obj":","},{"id":"T10012","span":{"begin":844,"end":845},"obj":"-RRB-"},{"id":"T10013","span":{"begin":845,"end":846},"obj":"."},{"id":"T10014","span":{"begin":846,"end":1017},"obj":"sentence"},{"id":"T10015","span":{"begin":847,"end":849},"obj":"IN"},{"id":"T10016","span":{"begin":913,"end":918},"obj":"VBN"},{"id":"T10017","span":{"begin":850,"end":853},"obj":"DT"},{"id":"T10018","span":{"begin":854,"end":859},"obj":"NN"},{"id":"T10019","span":{"begin":859,"end":861},"obj":","},{"id":"T10020","span":{"begin":861,"end":870},"obj":"JJ"},{"id":"T10021","span":{"begin":892,"end":897},"obj":"NNS"},{"id":"T10022","span":{"begin":871,"end":877},"obj":"JJ"},{"id":"T10023","span":{"begin":878,"end":883},"obj":"NN"},{"id":"T10024","span":{"begin":884,"end":891},"obj":"VBN"},{"id":"T10025","span":{"begin":883,"end":884},"obj":"HYPH"},{"id":"T10026","span":{"begin":898,"end":902},"obj":"VBP"},{"id":"T10027","span":{"begin":903,"end":907},"obj":"RB"},{"id":"T10028","span":{"begin":908,"end":912},"obj":"VBN"},{"id":"T10029","span":{"begin":919,"end":921},"obj":"IN"},{"id":"T10030","span":{"begin":922,"end":925},"obj":"DT"},{"id":"T10031","span":{"begin":926,"end":931},"obj":"NNS"},{"id":"T10032","span":{"begin":931,"end":933},"obj":","},{"id":"T10033","span":{"begin":933,"end":938},"obj":"WRB"},{"id":"T10034","span":{"begin":967,"end":976},"obj":"VBP"},{"id":"T10035","span":{"begin":939,"end":942},"obj":"DT"},{"id":"T10036","span":{"begin":943,"end":949},"obj":"NNS"},{"id":"T10037","span":{"begin":950,"end":952},"obj":"IN"},{"id":"T10038","span":{"begin":953,"end":956},"obj":"DT"},{"id":"T10039","span":{"begin":960,"end":966},"obj":"NN"},{"id":"T10040","span":{"begin":957,"end":959},"obj":"NN"},{"id":"T10041","span":{"begin":977,"end":981},"obj":"IN"},{"id":"T10042","span":{"begin":982,"end":985},"obj":"DT"},{"id":"T10043","span":{"begin":998,"end":1008},"obj":"NN"},{"id":"T10044","span":{"begin":986,"end":989},"obj":"NN"},{"id":"T10045","span":{"begin":990,"end":997},"obj":"NN"},{"id":"T10046","span":{"begin":1009,"end":1010},"obj":"-LRB-"},{"id":"T10047","span":{"begin":1013,"end":1015},"obj":"CD"},{"id":"T10048","span":{"begin":1010,"end":1012},"obj":"CD"},{"id":"T10049","span":{"begin":1012,"end":1013},"obj":","},{"id":"T10050","span":{"begin":1015,"end":1016},"obj":"-RRB-"},{"id":"T10051","span":{"begin":1016,"end":1017},"obj":"."},{"id":"T10052","span":{"begin":1017,"end":1188},"obj":"sentence"},{"id":"T10053","span":{"begin":1018,"end":1022},"obj":"RB"},{"id":"T10054","span":{"begin":1027,"end":1035},"obj":"VBD"},{"id":"T10055","span":{"begin":1022,"end":1024},"obj":","},{"id":"T10056","span":{"begin":1024,"end":1026},"obj":"PRP"},{"id":"T10057","span":{"begin":1036,"end":1045},"obj":"NN"},{"id":"T10058","span":{"begin":1046,"end":1054},"obj":"NNS"},{"id":"T10059","span":{"begin":1055,"end":1059},"obj":"RB"},{"id":"T10060","span":{"begin":1060,"end":1062},"obj":"IN"},{"id":"T10061","span":{"begin":1063,"end":1066},"obj":"DT"},{"id":"T10062","span":{"begin":1081,"end":1086},"obj":"NN"},{"id":"T10063","span":{"begin":1067,"end":1071},"obj":"RBR"},{"id":"T10064","span":{"begin":1072,"end":1080},"obj":"JJ"},{"id":"T10065","span":{"begin":1086,"end":1088},"obj":","},{"id":"T10066","span":{"begin":1088,"end":1091},"obj":"CC"},{"id":"T10067","span":{"begin":1092,"end":1097},"obj":"VBD"},{"id":"T10068","span":{"begin":1098,"end":1100},"obj":"DT"},{"id":"T10069","span":{"begin":1112,"end":1123},"obj":"NNS"},{"id":"T10070","span":{"begin":1101,"end":1111},"obj":"JJ"},{"id":"T10071","span":{"begin":1124,"end":1126},"obj":"IN"},{"id":"T10072","span":{"begin":1127,"end":1132},"obj":"NN"},{"id":"T10073","span":{"begin":1133,"end":1140},"obj":"IN"},{"id":"T10074","span":{"begin":1141,"end":1145},"obj":"NN"},{"id":"T10075","span":{"begin":1151,"end":1154},"obj":"NN"},{"id":"T10076","span":{"begin":1145,"end":1146},"obj":"HYPH"},{"id":"T10077","span":{"begin":1146,"end":1150},"obj":"NN"},{"id":"T10078","span":{"begin":1150,"end":1151},"obj":"HYPH"},{"id":"T10079","span":{"begin":1155,"end":1162},"obj":"NNS"},{"id":"T10080","span":{"begin":1163,"end":1166},"obj":"CC"},{"id":"T10081","span":{"begin":1167,"end":1175},"obj":"NNS"},{"id":"T10082","span":{"begin":1176,"end":1177},"obj":"-LRB-"},{"id":"T10083","span":{"begin":1185,"end":1186},"obj":"NN"},{"id":"T10084","span":{"begin":1177,"end":1181},"obj":"NN"},{"id":"T10085","span":{"begin":1182,"end":1184},"obj":"NN"},{"id":"T10086","span":{"begin":1184,"end":1185},"obj":","},{"id":"T10087","span":{"begin":1186,"end":1187},"obj":"-RRB-"},{"id":"T10088","span":{"begin":1187,"end":1188},"obj":"."},{"id":"T10089","span":{"begin":1188,"end":1376},"obj":"sentence"},{"id":"T10090","span":{"begin":1189,"end":1195},"obj":"JJ"},{"id":"T10091","span":{"begin":1269,"end":1271},"obj":"VBZ"},{"id":"T10092","span":{"begin":1196,"end":1198},"obj":"IN"},{"id":"T10093","span":{"begin":1199,"end":1203},"obj":"NN"},{"id":"T10094","span":{"begin":1209,"end":1212},"obj":"NN"},{"id":"T10095","span":{"begin":1203,"end":1204},"obj":"HYPH"},{"id":"T10096","span":{"begin":1204,"end":1208},"obj":"NN"},{"id":"T10097","span":{"begin":1208,"end":1209},"obj":"HYPH"},{"id":"T10098","span":{"begin":1220,"end":1227},"obj":"NNS"},{"id":"T10099","span":{"begin":1213,"end":1219},"obj":"NN"},{"id":"T10100","span":{"begin":1227,"end":1229},"obj":","},{"id":"T10101","span":{"begin":1229,"end":1238},"obj":"VBN"},{"id":"T10102","span":{"begin":1239,"end":1248},"obj":"NN"},{"id":"T10103","span":{"begin":1249,"end":1251},"obj":"IN"},{"id":"T10104","span":{"begin":1252,"end":1254},"obj":"NN"},{"id":"T10105","span":{"begin":1255,"end":1262},"obj":"VBN"},{"id":"T10106","span":{"begin":1254,"end":1255},"obj":"HYPH"},{"id":"T10107","span":{"begin":1263,"end":1268},"obj":"NNS"},{"id":"T10108","span":{"begin":1272,"end":1275},"obj":"RB"},{"id":"T10109","span":{"begin":1276,"end":1287},"obj":"JJ"},{"id":"T10110","span":{"begin":1288,"end":1291},"obj":"IN"},{"id":"T10111","span":{"begin":1292,"end":1295},"obj":"DT"},{"id":"T10112","span":{"begin":1305,"end":1312},"obj":"NNS"},{"id":"T10113","span":{"begin":1296,"end":1304},"obj":"VBN"},{"id":"T10114","span":{"begin":1313,"end":1315},"obj":"IN"},{"id":"T10115","span":{"begin":1316,"end":1319},"obj":"DT"},{"id":"T10116","span":{"begin":1324,"end":1333},"obj":"NN"},{"id":"T10117","span":{"begin":1320,"end":1323},"obj":"NN"},{"id":"T10118","span":{"begin":1334,"end":1336},"obj":"IN"},{"id":"T10119","span":{"begin":1337,"end":1350},"obj":"VBG"},{"id":"T10120","span":{"begin":1356,"end":1363},"obj":"NNS"},{"id":"T10121","span":{"begin":1351,"end":1355},"obj":"NN"},{"id":"T10122","span":{"begin":1364,"end":1365},"obj":"-LRB-"},{"id":"T10123","span":{"begin":1373,"end":1374},"obj":"NN"},{"id":"T10124","span":{"begin":1365,"end":1369},"obj":"NN"},{"id":"T10125","span":{"begin":1370,"end":1372},"obj":"NN"},{"id":"T10126","span":{"begin":1372,"end":1373},"obj":","},{"id":"T10127","span":{"begin":1374,"end":1375},"obj":"-RRB-"},{"id":"T10128","span":{"begin":1375,"end":1376},"obj":"."}],"relations":[{"id":"R2822","pred":"mark","subj":"T9852","obj":"T9853"},{"id":"R2823","pred":"advcl","subj":"T9853","obj":"T9854"},{"id":"R2824","pred":"advmod","subj":"T9855","obj":"T9853"},{"id":"R2825","pred":"punct","subj":"T9856","obj":"T9854"},{"id":"R2826","pred":"compound","subj":"T9857","obj":"T9858"},{"id":"R2827","pred":"compound","subj":"T9858","obj":"T9862"},{"id":"R2828","pred":"punct","subj":"T9859","obj":"T9858"},{"id":"R2829","pred":"compound","subj":"T9860","obj":"T9858"},{"id":"R2830","pred":"punct","subj":"T9861","obj":"T9858"},{"id":"R2831","pred":"nsubj","subj":"T9862","obj":"T9854"},{"id":"R2832","pred":"det","subj":"T9863","obj":"T9864"},{"id":"R2833","pred":"dobj","subj":"T9864","obj":"T9854"},{"id":"R2834","pred":"amod","subj":"T9865","obj":"T9864"},{"id":"R2835","pred":"prep","subj":"T9866","obj":"T9864"},{"id":"R2836","pred":"npadvmod","subj":"T9867","obj":"T9868"},{"id":"R2837","pred":"amod","subj":"T9868","obj":"T9870"},{"id":"R2838","pred":"punct","subj":"T9869","obj":"T9868"},{"id":"R2839","pred":"pobj","subj":"T9870","obj":"T9866"},{"id":"R2840","pred":"prep","subj":"T9871","obj":"T9864"},{"id":"R2841","pred":"det","subj":"T9872","obj":"T9873"},{"id":"R2842","pred":"pobj","subj":"T9873","obj":"T9871"},{"id":"R2843","pred":"prep","subj":"T9874","obj":"T9873"},{"id":"R2844","pred":"det","subj":"T9875","obj":"T9876"},{"id":"R2845","pred":"pobj","subj":"T9876","obj":"T9874"},{"id":"R2846","pred":"amod","subj":"T9877","obj":"T9876"},{"id":"R2847","pred":"prep","subj":"T9878","obj":"T9873"},{"id":"R2848","pred":"det","subj":"T9879","obj":"T9880"},{"id":"R2849","pred":"pobj","subj":"T9880","obj":"T9878"},{"id":"R2850","pred":"prep","subj":"T9881","obj":"T9880"},{"id":"R2851","pred":"amod","subj":"T9882","obj":"T9883"},{"id":"R2852","pred":"pobj","subj":"T9883","obj":"T9881"},{"id":"R2853","pred":"cc","subj":"T9884","obj":"T9882"},{"id":"R2854","pred":"conj","subj":"T9885","obj":"T9882"},{"id":"R2855","pred":"punct","subj":"T9886","obj":"T9854"},{"id":"R2856","pred":"aux","subj":"T9888","obj":"T9889"},{"id":"R2857","pred":"advcl","subj":"T9889","obj":"T9890"},{"id":"R2858","pred":"mark","subj":"T9891","obj":"T9892"},{"id":"R2859","pred":"ccomp","subj":"T9892","obj":"T9889"},{"id":"R2860","pred":"det","subj":"T9893","obj":"T9894"},{"id":"R2861","pred":"nsubj","subj":"T9894","obj":"T9892"},{"id":"R2862","pred":"preconj","subj":"T9895","obj":"T9896"},{"id":"R2863","pred":"prep","subj":"T9896","obj":"T9892"},{"id":"R2864","pred":"amod","subj":"T9897","obj":"T9898"},{"id":"R2865","pred":"pobj","subj":"T9898","obj":"T9896"},{"id":"R2866","pred":"compound","subj":"T9899","obj":"T9898"},{"id":"R2867","pred":"cc","subj":"T9900","obj":"T9898"},{"id":"R2868","pred":"amod","subj":"T9901","obj":"T9902"},{"id":"R2869","pred":"conj","subj":"T9902","obj":"T9898"},{"id":"R2870","pred":"punct","subj":"T9903","obj":"T9890"},{"id":"R2871","pred":"nsubj","subj":"T9904","obj":"T9890"},{"id":"R2872","pred":"nmod","subj":"T9905","obj":"T9906"},{"id":"R2873","pred":"dobj","subj":"T9906","obj":"T9890"},{"id":"R2874","pred":"cc","subj":"T9907","obj":"T9905"},{"id":"R2875","pred":"conj","subj":"T9908","obj":"T9905"},{"id":"R2876","pred":"punct","subj":"T9909","obj":"T9890"},{"id":"R2877","pred":"advmod","subj":"T9910","obj":"T9890"},{"id":"R2878","pred":"punct","subj":"T9911","obj":"T9890"},{"id":"R2879","pred":"mark","subj":"T9913","obj":"T9914"},{"id":"R2880","pred":"advcl","subj":"T9914","obj":"T9919"},{"id":"R2881","pred":"compound","subj":"T9915","obj":"T9916"},{"id":"R2882","pred":"nsubjpass","subj":"T9916","obj":"T9914"},{"id":"R2883","pred":"auxpass","subj":"T9917","obj":"T9914"},{"id":"R2884","pred":"neg","subj":"T9918","obj":"T9914"},{"id":"R2885","pred":"prep","subj":"T9920","obj":"T9914"},{"id":"R2886","pred":"compound","subj":"T9921","obj":"T9922"},{"id":"R2887","pred":"pobj","subj":"T9922","obj":"T9920"},{"id":"R2888","pred":"punct","subj":"T9923","obj":"T9924"},{"id":"R2889","pred":"parataxis","subj":"T9924","obj":"T9914"},{"id":"R2890","pred":"nsubj","subj":"T9925","obj":"T9924"},{"id":"R2891","pred":"neg","subj":"T9926","obj":"T9924"},{"id":"R2892","pred":"punct","subj":"T9927","obj":"T9924"},{"id":"R2893","pred":"punct","subj":"T9928","obj":"T9919"},{"id":"R2894","pred":"nsubj","subj":"T9929","obj":"T9919"},{"id":"R2895","pred":"aux","subj":"T9930","obj":"T9919"},{"id":"R2896","pred":"det","subj":"T9931","obj":"T9932"},{"id":"R2897","pred":"dobj","subj":"T9932","obj":"T9919"},{"id":"R2898","pred":"amod","subj":"T9933","obj":"T9932"},{"id":"R2899","pred":"prep","subj":"T9934","obj":"T9932"},{"id":"R2900","pred":"det","subj":"T9935","obj":"T9936"},{"id":"R2901","pred":"pobj","subj":"T9936","obj":"T9934"},{"id":"R2902","pred":"prep","subj":"T9937","obj":"T9936"},{"id":"R2903","pred":"npadvmod","subj":"T9938","obj":"T9939"},{"id":"R2904","pred":"amod","subj":"T9939","obj":"T9940"},{"id":"R2905","pred":"pobj","subj":"T9940","obj":"T9937"},{"id":"R2906","pred":"prep","subj":"T9941","obj":"T9932"},{"id":"R2907","pred":"pobj","subj":"T9942","obj":"T9941"},{"id":"R2908","pred":"acl","subj":"T9943","obj":"T9942"},{"id":"R2909","pred":"det","subj":"T9944","obj":"T9945"},{"id":"R2910","pred":"dobj","subj":"T9945","obj":"T9943"},{"id":"R2911","pred":"amod","subj":"T9946","obj":"T9945"},{"id":"R2912","pred":"prep","subj":"T9947","obj":"T9942"},{"id":"R2913","pred":"det","subj":"T9948","obj":"T9949"},{"id":"R2914","pred":"pobj","subj":"T9949","obj":"T9947"},{"id":"R2915","pred":"advmod","subj":"T9950","obj":"T9951"},{"id":"R2916","pred":"relcl","subj":"T9951","obj":"T9949"},{"id":"R2917","pred":"det","subj":"T9952","obj":"T9953"},{"id":"R2918","pred":"nsubj","subj":"T9953","obj":"T9951"},{"id":"R2919","pred":"compound","subj":"T9954","obj":"T9953"},{"id":"R2920","pred":"punct","subj":"T9955","obj":"T9953"},{"id":"R2921","pred":"punct","subj":"T9956","obj":"T9957"},{"id":"R2922","pred":"parataxis","subj":"T9957","obj":"T9951"},{"id":"R2923","pred":"compound","subj":"T9958","obj":"T9957"},{"id":"R2924","pred":"punct","subj":"T9959","obj":"T9960"},{"id":"R2925","pred":"prep","subj":"T9960","obj":"T9957"},{"id":"R2926","pred":"punct","subj":"T9961","obj":"T9957"},{"id":"R2927","pred":"punct","subj":"T9962","obj":"T9919"},{"id":"R2928","pred":"amod","subj":"T9964","obj":"T9965"},{"id":"R2929","pred":"nsubj","subj":"T9965","obj":"T9966"},{"id":"R2930","pred":"ccomp","subj":"T9966","obj":"T9973"},{"id":"R2931","pred":"prep","subj":"T9967","obj":"T9965"},{"id":"R2932","pred":"npadvmod","subj":"T9968","obj":"T9969"},{"id":"R2933","pred":"amod","subj":"T9969","obj":"T9972"},{"id":"R2934","pred":"cc","subj":"T9970","obj":"T9968"},{"id":"R2935","pred":"conj","subj":"T9971","obj":"T9968"},{"id":"R2936","pred":"pobj","subj":"T9972","obj":"T9967"},{"id":"R2937","pred":"mark","subj":"T9974","obj":"T9975"},{"id":"R2938","pred":"ccomp","subj":"T9975","obj":"T9966"},{"id":"R2939","pred":"det","subj":"T9976","obj":"T9977"},{"id":"R2940","pred":"nsubj","subj":"T9977","obj":"T9975"},{"id":"R2941","pred":"amod","subj":"T9978","obj":"T9979"},{"id":"R2942","pred":"attr","subj":"T9979","obj":"T9975"},{"id":"R2943","pred":"punct","subj":"T9980","obj":"T9973"},{"id":"R2944","pred":"det","subj":"T9981","obj":"T9982"},{"id":"R2945","pred":"nsubj","subj":"T9982","obj":"T9973"},{"id":"R2946","pred":"advmod","subj":"T9983","obj":"T9973"},{"id":"R2947","pred":"advmod","subj":"T9984","obj":"T9985"},{"id":"R2948","pred":"acomp","subj":"T9985","obj":"T9973"},{"id":"R2949","pred":"prep","subj":"T9986","obj":"T9973"},{"id":"R2950","pred":"pobj","subj":"T9987","obj":"T9986"},{"id":"R2951","pred":"punct","subj":"T9988","obj":"T9973"},{"id":"R2952","pred":"det","subj":"T9990","obj":"T9991"},{"id":"R2953","pred":"nsubjpass","subj":"T9991","obj":"T9992"},{"id":"R2954","pred":"auxpass","subj":"T9993","obj":"T9992"},{"id":"R2955","pred":"prep","subj":"T9994","obj":"T9992"},{"id":"R2956","pred":"pcomp","subj":"T9995","obj":"T9994"},{"id":"R2957","pred":"dobj","subj":"T9996","obj":"T9995"},{"id":"R2958","pred":"prep","subj":"T9997","obj":"T9995"},{"id":"R2959","pred":"amod","subj":"T9998","obj":"T9999"},{"id":"R2960","pred":"pobj","subj":"T9999","obj":"T9997"},{"id":"R2961","pred":"punct","subj":"T10000","obj":"T9999"},{"id":"R2962","pred":"nummod","subj":"T10001","obj":"T9999"},{"id":"R2963","pred":"punct","subj":"T10002","obj":"T9999"},{"id":"R2964","pred":"det","subj":"T10003","obj":"T10004"},{"id":"R2965","pred":"appos","subj":"T10004","obj":"T9999"},{"id":"R2966","pred":"prep","subj":"T10005","obj":"T10004"},{"id":"R2967","pred":"pobj","subj":"T10006","obj":"T10005"},{"id":"R2968","pred":"punct","subj":"T10007","obj":"T10008"},{"id":"R2969","pred":"parataxis","subj":"T10008","obj":"T9997"},{"id":"R2970","pred":"nmod","subj":"T10009","obj":"T10008"},{"id":"R2971","pred":"nmod","subj":"T10010","obj":"T10008"},{"id":"R2972","pred":"punct","subj":"T10011","obj":"T10008"},{"id":"R2973","pred":"punct","subj":"T10012","obj":"T10008"},{"id":"R2974","pred":"punct","subj":"T10013","obj":"T9992"},{"id":"R2975","pred":"prep","subj":"T10015","obj":"T10016"},{"id":"R2976","pred":"det","subj":"T10017","obj":"T10018"},{"id":"R2977","pred":"pobj","subj":"T10018","obj":"T10015"},{"id":"R2978","pred":"punct","subj":"T10019","obj":"T10016"},{"id":"R2979","pred":"amod","subj":"T10020","obj":"T10021"},{"id":"R2980","pred":"nsubjpass","subj":"T10021","obj":"T10016"},{"id":"R2981","pred":"amod","subj":"T10022","obj":"T10023"},{"id":"R2982","pred":"npadvmod","subj":"T10023","obj":"T10024"},{"id":"R2983","pred":"amod","subj":"T10024","obj":"T10021"},{"id":"R2984","pred":"punct","subj":"T10025","obj":"T10024"},{"id":"R2985","pred":"aux","subj":"T10026","obj":"T10016"},{"id":"R2986","pred":"advmod","subj":"T10027","obj":"T10016"},{"id":"R2987","pred":"auxpass","subj":"T10028","obj":"T10016"},{"id":"R2988","pred":"prep","subj":"T10029","obj":"T10016"},{"id":"R2989","pred":"det","subj":"T10030","obj":"T10031"},{"id":"R2990","pred":"pobj","subj":"T10031","obj":"T10029"},{"id":"R2991","pred":"punct","subj":"T10032","obj":"T10031"},{"id":"R2992","pred":"advmod","subj":"T10033","obj":"T10034"},{"id":"R2993","pred":"relcl","subj":"T10034","obj":"T10031"},{"id":"R2994","pred":"det","subj":"T10035","obj":"T10036"},{"id":"R2995","pred":"nsubj","subj":"T10036","obj":"T10034"},{"id":"R2996","pred":"prep","subj":"T10037","obj":"T10036"},{"id":"R2997","pred":"det","subj":"T10038","obj":"T10039"},{"id":"R2998","pred":"pobj","subj":"T10039","obj":"T10037"},{"id":"R2999","pred":"compound","subj":"T10040","obj":"T10039"},{"id":"R3000","pred":"prep","subj":"T10041","obj":"T10034"},{"id":"R3001","pred":"det","subj":"T10042","obj":"T10043"},{"id":"R3002","pred":"pobj","subj":"T10043","obj":"T10041"},{"id":"R3003","pred":"compound","subj":"T10044","obj":"T10043"},{"id":"R3004","pred":"compound","subj":"T10045","obj":"T10043"},{"id":"R3005","pred":"punct","subj":"T10046","obj":"T10047"},{"id":"R3006","pred":"parataxis","subj":"T10047","obj":"T10016"},{"id":"R3007","pred":"nummod","subj":"T10048","obj":"T10047"},{"id":"R3008","pred":"punct","subj":"T10049","obj":"T10047"},{"id":"R3009","pred":"punct","subj":"T10050","obj":"T10047"},{"id":"R3010","pred":"punct","subj":"T10051","obj":"T10016"},{"id":"R3011","pred":"advmod","subj":"T10053","obj":"T10054"},{"id":"R3012","pred":"punct","subj":"T10055","obj":"T10054"},{"id":"R3013","pred":"nsubj","subj":"T10056","obj":"T10054"},{"id":"R3014","pred":"compound","subj":"T10057","obj":"T10058"},{"id":"R3015","pred":"dobj","subj":"T10058","obj":"T10054"},{"id":"R3016","pred":"advmod","subj":"T10059","obj":"T10060"},{"id":"R3017","pred":"prep","subj":"T10060","obj":"T10054"},{"id":"R3018","pred":"det","subj":"T10061","obj":"T10062"},{"id":"R3019","pred":"pobj","subj":"T10062","obj":"T10060"},{"id":"R3020","pred":"advmod","subj":"T10063","obj":"T10064"},{"id":"R3021","pred":"amod","subj":"T10064","obj":"T10062"},{"id":"R3022","pred":"punct","subj":"T10065","obj":"T10054"},{"id":"R3023","pred":"cc","subj":"T10066","obj":"T10054"},{"id":"R3024","pred":"conj","subj":"T10067","obj":"T10054"},{"id":"R3025","pred":"det","subj":"T10068","obj":"T10069"},{"id":"R3026","pred":"dobj","subj":"T10069","obj":"T10067"},{"id":"R3027","pred":"amod","subj":"T10070","obj":"T10069"},{"id":"R3028","pred":"prep","subj":"T10071","obj":"T10069"},{"id":"R3029","pred":"pobj","subj":"T10072","obj":"T10071"},{"id":"R3030","pred":"prep","subj":"T10073","obj":"T10069"},{"id":"R3031","pred":"compound","subj":"T10074","obj":"T10075"},{"id":"R3032","pred":"compound","subj":"T10075","obj":"T10079"},{"id":"R3033","pred":"punct","subj":"T10076","obj":"T10075"},{"id":"R3034","pred":"compound","subj":"T10077","obj":"T10075"},{"id":"R3035","pred":"punct","subj":"T10078","obj":"T10075"},{"id":"R3036","pred":"pobj","subj":"T10079","obj":"T10073"},{"id":"R3037","pred":"cc","subj":"T10080","obj":"T10079"},{"id":"R3038","pred":"conj","subj":"T10081","obj":"T10079"},{"id":"R3039","pred":"punct","subj":"T10082","obj":"T10083"},{"id":"R3040","pred":"parataxis","subj":"T10083","obj":"T10067"},{"id":"R3041","pred":"nmod","subj":"T10084","obj":"T10083"},{"id":"R3042","pred":"nmod","subj":"T10085","obj":"T10083"},{"id":"R3043","pred":"punct","subj":"T10086","obj":"T10083"},{"id":"R3044","pred":"punct","subj":"T10087","obj":"T10083"},{"id":"R3045","pred":"punct","subj":"T10088","obj":"T10054"},{"id":"R3046","pred":"advcl","subj":"T10090","obj":"T10091"},{"id":"R3047","pred":"prep","subj":"T10092","obj":"T10090"},{"id":"R3048","pred":"compound","subj":"T10093","obj":"T10094"},{"id":"R3049","pred":"compound","subj":"T10094","obj":"T10098"},{"id":"R3050","pred":"punct","subj":"T10095","obj":"T10094"},{"id":"R3051","pred":"compound","subj":"T10096","obj":"T10094"},{"id":"R3052","pred":"punct","subj":"T10097","obj":"T10094"},{"id":"R3053","pred":"pobj","subj":"T10098","obj":"T10092"},{"id":"R3054","pred":"compound","subj":"T10099","obj":"T10098"},{"id":"R3055","pred":"punct","subj":"T10100","obj":"T10091"},{"id":"R3056","pred":"amod","subj":"T10101","obj":"T10102"},{"id":"R3057","pred":"nsubj","subj":"T10102","obj":"T10091"},{"id":"R3058","pred":"prep","subj":"T10103","obj":"T10102"},{"id":"R3059","pred":"npadvmod","subj":"T10104","obj":"T10105"},{"id":"R3060","pred":"amod","subj":"T10105","obj":"T10107"},{"id":"R3061","pred":"punct","subj":"T10106","obj":"T10105"},{"id":"R3062","pred":"pobj","subj":"T10107","obj":"T10103"},{"id":"R3063","pred":"neg","subj":"T10108","obj":"T10091"},{"id":"R3064","pred":"acomp","subj":"T10109","obj":"T10091"},{"id":"R3065","pred":"prep","subj":"T10110","obj":"T10109"},{"id":"R3066","pred":"det","subj":"T10111","obj":"T10112"},{"id":"R3067","pred":"pobj","subj":"T10112","obj":"T10110"},{"id":"R3068","pred":"amod","subj":"T10113","obj":"T10112"},{"id":"R3069","pred":"prep","subj":"T10114","obj":"T10112"},{"id":"R3070","pred":"det","subj":"T10115","obj":"T10116"},{"id":"R3071","pred":"pobj","subj":"T10116","obj":"T10114"},{"id":"R3072","pred":"compound","subj":"T10117","obj":"T10116"},{"id":"R3073","pred":"prep","subj":"T10118","obj":"T10116"},{"id":"R3074","pred":"amod","subj":"T10119","obj":"T10120"},{"id":"R3075","pred":"pobj","subj":"T10120","obj":"T10118"},{"id":"R3076","pred":"compound","subj":"T10121","obj":"T10120"},{"id":"R3077","pred":"punct","subj":"T10122","obj":"T10123"},{"id":"R3078","pred":"parataxis","subj":"T10123","obj":"T10091"},{"id":"R3079","pred":"nmod","subj":"T10124","obj":"T10123"},{"id":"R3080","pred":"nmod","subj":"T10125","obj":"T10123"},{"id":"R3081","pred":"punct","subj":"T10126","obj":"T10123"},{"id":"R3082","pred":"punct","subj":"T10127","obj":"T10123"},{"id":"R3083","pred":"punct","subj":"T10128","obj":"T10091"}],"text":"As described above, Alk5/Wnt1-Cre mutants displayed an inadequate amount of AP-septal tissue in the base of the aortic sac between the origins of 4th and 6th PAAs. To analyze whether this phenotype resulted either from defective CNCC proliferation or inappropriate apoptosis, we used BrdU and TUNEL staining, respectively. While CNCC proliferation was not affected in Alk5 mutants (data not shown), we could detect a dramatic increase in the number of TUNEL positive cells in tissues surrounding the aortic sac including the site where the AP-septum forms (Fig. 7A–C). Dual staining for lacZ and TUNEL positive cells demonstrated that these cells were postmigratory CNCCs; this phenotype was already clearly detectable at E10.5. These results were confirmed by using immunostaining for cleaved caspase-3, another marker for apoptosis (Fig. 7I,J). In the chick, apoptotic neural crest-derived cells have also been found at the sites, where the prongs of the AP septum penetrate into the OFT cushion mesenchyme [25,26]. Thus, we compared apoptosis patterns also on the more proximal level, but found no detectable differences at E11.0 between Alk5/Wnt1-Cre mutants and controls (Fig. 7D,E). Unlike in Alk5/Wnt1-Cre mutant embryos, increased apoptosis of NC-derived cells is not responsible for the observed defects in the OFT septation in corresponding Alk2 mutants (Fig. 7C,E)."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T9792","span":{"begin":656,"end":665},"obj":"GO_EXT:biological_movement_or_translocation_process"},{"id":"T9793","span":{"begin":786,"end":793},"obj":"MOP:0000780"},{"id":"T9794","span":{"begin":786,"end":803},"obj":"PR_EXT:000018762"},{"id":"T9795","span":{"begin":813,"end":819},"obj":"CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag"},{"id":"T9796","span":{"begin":824,"end":833},"obj":"GO:0006915"},{"id":"T9797","span":{"begin":854,"end":859},"obj":"NCBITaxon_UBERON_EXT:chick"},{"id":"T9798","span":{"begin":861,"end":870},"obj":"GO_PATO_EXT:apoptotic_process_or_quality"},{"id":"T9799","span":{"begin":861,"end":870},"obj":"_FRAGMENT"},{"id":"T9800","span":{"begin":892,"end":897},"obj":"CL:0000445"},{"id":"T9801","span":{"begin":871,"end":883},"obj":"UBERON:0002342"},{"id":"T9802","span":{"begin":892,"end":897},"obj":"CL_GO_EXT:cell"},{"id":"T9803","span":{"begin":957,"end":966},"obj":"UBERON:0006207"},{"id":"T9804","span":{"begin":986,"end":997},"obj":"UBERON:0005967"},{"id":"T9805","span":{"begin":998,"end":1008},"obj":"UBERON:0003104"},{"id":"T9806","span":{"begin":1036,"end":1045},"obj":"GO:0006915"},{"id":"T9807","span":{"begin":1141,"end":1145},"obj":"PR_EXT:000000047"},{"id":"T9808","span":{"begin":1146,"end":1150},"obj":"PR_EXT:000017435"},{"id":"T9809","span":{"begin":1155,"end":1162},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T9810","span":{"begin":1199,"end":1203},"obj":"PR_EXT:000000047"},{"id":"T9811","span":{"begin":1204,"end":1208},"obj":"PR_EXT:000017435"},{"id":"T9812","span":{"begin":1213,"end":1219},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T9813","span":{"begin":1220,"end":1227},"obj":"UBERON:0000922"},{"id":"T9814","span":{"begin":1239,"end":1251},"obj":"_FRAGMENT"},{"id":"T9815","span":{"begin":1263,"end":1268},"obj":"GO:0006915"},{"id":"T9816","span":{"begin":1252,"end":1254},"obj":"UBERON:0002342"},{"id":"T9817","span":{"begin":1263,"end":1268},"obj":"CL_GO_EXT:cell"},{"id":"T9818","span":{"begin":1320,"end":1323},"obj":"UBERON:0004145"},{"id":"T9819","span":{"begin":1351,"end":1355},"obj":"PR_EXT:000000067"},{"id":"T9820","span":{"begin":1356,"end":1363},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T9770","span":{"begin":20,"end":24},"obj":"PR_EXT:000000047"},{"id":"T9771","span":{"begin":25,"end":29},"obj":"PR_EXT:000017435"},{"id":"T9772","span":{"begin":34,"end":41},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T9773","span":{"begin":76,"end":85},"obj":"UBERON:0006207"},{"id":"T9774","span":{"begin":86,"end":92},"obj":"UBERON:0000479"},{"id":"T9775","span":{"begin":112,"end":122},"obj":"UBERON:0005432"},{"id":"T9776","span":{"begin":146,"end":149},"obj":"_FRAGMENT"},{"id":"T9777","span":{"begin":158,"end":162},"obj":"UBERON:0003121"},{"id":"T9778","span":{"begin":154,"end":162},"obj":"UBERON:0003123"},{"id":"T9779","span":{"begin":234,"end":247},"obj":"GO:0008283"},{"id":"T9780","span":{"begin":265,"end":274},"obj":"GO:0006915"},{"id":"T9781","span":{"begin":284,"end":288},"obj":"CHEBI:472552"},{"id":"T9782","span":{"begin":334,"end":347},"obj":"GO:0008283"},{"id":"T9783","span":{"begin":368,"end":372},"obj":"PR_EXT:000000047"},{"id":"T9784","span":{"begin":373,"end":380},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T9785","span":{"begin":467,"end":472},"obj":"CL_GO_EXT:cell"},{"id":"T9786","span":{"begin":476,"end":483},"obj":"UBERON:0000479"},{"id":"T9787","span":{"begin":500,"end":510},"obj":"UBERON:0005432"},{"id":"T9788","span":{"begin":540,"end":549},"obj":"UBERON:0006207"},{"id":"T9789","span":{"begin":587,"end":591},"obj":"PR_EXT:000033987"},{"id":"T9790","span":{"begin":611,"end":616},"obj":"CL_GO_EXT:cell"},{"id":"T9791","span":{"begin":641,"end":646},"obj":"CL_GO_EXT:cell"}],"relations":[{"id":"R2805","pred":"_lexicallyChainedTo","subj":"T9777","obj":"T9776"},{"id":"R2806","pred":"_lexicallyChainedTo","subj":"T9800","obj":"T9799"},{"id":"R2807","pred":"_lexicallyChainedTo","subj":"T9815","obj":"T9814"}],"text":"As described above, Alk5/Wnt1-Cre mutants displayed an inadequate amount of AP-septal tissue in the base of the aortic sac between the origins of 4th and 6th PAAs. To analyze whether this phenotype resulted either from defective CNCC proliferation or inappropriate apoptosis, we used BrdU and TUNEL staining, respectively. While CNCC proliferation was not affected in Alk5 mutants (data not shown), we could detect a dramatic increase in the number of TUNEL positive cells in tissues surrounding the aortic sac including the site where the AP-septum forms (Fig. 7A–C). Dual staining for lacZ and TUNEL positive cells demonstrated that these cells were postmigratory CNCCs; this phenotype was already clearly detectable at E10.5. These results were confirmed by using immunostaining for cleaved caspase-3, another marker for apoptosis (Fig. 7I,J). In the chick, apoptotic neural crest-derived cells have also been found at the sites, where the prongs of the AP septum penetrate into the OFT cushion mesenchyme [25,26]. Thus, we compared apoptosis patterns also on the more proximal level, but found no detectable differences at E11.0 between Alk5/Wnt1-Cre mutants and controls (Fig. 7D,E). Unlike in Alk5/Wnt1-Cre mutant embryos, increased apoptosis of NC-derived cells is not responsible for the observed defects in the OFT septation in corresponding Alk2 mutants (Fig. 7C,E)."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T9728","span":{"begin":500,"end":510},"obj":"UBERON:0005432"},{"id":"T9729","span":{"begin":540,"end":549},"obj":"UBERON:0006207"},{"id":"T9730","span":{"begin":587,"end":591},"obj":"PR:000033987"},{"id":"T9731","span":{"begin":786,"end":793},"obj":"MOP:0000780"},{"id":"T9732","span":{"begin":786,"end":803},"obj":"PR:000018762"},{"id":"T9733","span":{"begin":824,"end":833},"obj":"GO:0006915"},{"id":"T9734","span":{"begin":861,"end":870},"obj":"_FRAGMENT"},{"id":"T9735","span":{"begin":892,"end":897},"obj":"CL:0000445"},{"id":"T9736","span":{"begin":871,"end":883},"obj":"UBERON:0002342"},{"id":"T9737","span":{"begin":957,"end":966},"obj":"UBERON:0006207"},{"id":"T9738","span":{"begin":986,"end":997},"obj":"UBERON:0005967"},{"id":"T9739","span":{"begin":998,"end":1008},"obj":"UBERON:0003104"},{"id":"T9740","span":{"begin":1036,"end":1045},"obj":"GO:0006915"},{"id":"T9741","span":{"begin":1141,"end":1145},"obj":"PR:000000047"},{"id":"T9742","span":{"begin":1146,"end":1150},"obj":"PR:000017435"},{"id":"T9743","span":{"begin":1199,"end":1203},"obj":"PR:000000047"},{"id":"T9744","span":{"begin":1204,"end":1208},"obj":"PR:000017435"},{"id":"T9745","span":{"begin":1220,"end":1227},"obj":"UBERON:0000922"},{"id":"T9746","span":{"begin":1239,"end":1251},"obj":"_FRAGMENT"},{"id":"T9747","span":{"begin":1263,"end":1268},"obj":"GO:0006915"},{"id":"T9748","span":{"begin":1252,"end":1254},"obj":"UBERON:0002342"},{"id":"T9749","span":{"begin":1320,"end":1323},"obj":"UBERON:0004145"},{"id":"T9750","span":{"begin":1351,"end":1355},"obj":"PR:000000067"},{"id":"T9714","span":{"begin":20,"end":24},"obj":"PR:000000047"},{"id":"T9715","span":{"begin":25,"end":29},"obj":"PR:000017435"},{"id":"T9716","span":{"begin":76,"end":85},"obj":"UBERON:0006207"},{"id":"T9717","span":{"begin":86,"end":92},"obj":"UBERON:0000479"},{"id":"T9718","span":{"begin":112,"end":122},"obj":"UBERON:0005432"},{"id":"T9719","span":{"begin":146,"end":149},"obj":"_FRAGMENT"},{"id":"T9720","span":{"begin":158,"end":162},"obj":"UBERON:0003121"},{"id":"T9721","span":{"begin":154,"end":162},"obj":"UBERON:0003123"},{"id":"T9722","span":{"begin":234,"end":247},"obj":"GO:0008283"},{"id":"T9723","span":{"begin":265,"end":274},"obj":"GO:0006915"},{"id":"T9724","span":{"begin":284,"end":288},"obj":"CHEBI:472552"},{"id":"T9725","span":{"begin":334,"end":347},"obj":"GO:0008283"},{"id":"T9726","span":{"begin":368,"end":372},"obj":"PR:000000047"},{"id":"T9727","span":{"begin":476,"end":483},"obj":"UBERON:0000479"}],"relations":[{"id":"R2801","pred":"_lexicallyChainedTo","subj":"T9735","obj":"T9734"},{"id":"R2802","pred":"_lexicallyChainedTo","subj":"T9747","obj":"T9746"},{"id":"R2800","pred":"_lexicallyChainedTo","subj":"T9720","obj":"T9719"}],"text":"As described above, Alk5/Wnt1-Cre mutants displayed an inadequate amount of AP-septal tissue in the base of the aortic sac between the origins of 4th and 6th PAAs. To analyze whether this phenotype resulted either from defective CNCC proliferation or inappropriate apoptosis, we used BrdU and TUNEL staining, respectively. While CNCC proliferation was not affected in Alk5 mutants (data not shown), we could detect a dramatic increase in the number of TUNEL positive cells in tissues surrounding the aortic sac including the site where the AP-septum forms (Fig. 7A–C). Dual staining for lacZ and TUNEL positive cells demonstrated that these cells were postmigratory CNCCs; this phenotype was already clearly detectable at E10.5. These results were confirmed by using immunostaining for cleaved caspase-3, another marker for apoptosis (Fig. 7I,J). In the chick, apoptotic neural crest-derived cells have also been found at the sites, where the prongs of the AP septum penetrate into the OFT cushion mesenchyme [25,26]. Thus, we compared apoptosis patterns also on the more proximal level, but found no detectable differences at E11.0 between Alk5/Wnt1-Cre mutants and controls (Fig. 7D,E). Unlike in Alk5/Wnt1-Cre mutant embryos, increased apoptosis of NC-derived cells is not responsible for the observed defects in the OFT septation in corresponding Alk2 mutants (Fig. 7C,E)."}