> top > docs > PMC:1630711 > spans > 33913-40653 > annotations

PMC:1630711 / 33913-40653 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

Id Subject Object Predicate Lexical cue
T14828 23-33 NN denotes Generation
T14829 34-36 IN denotes of
T14830 37-43 NN denotes Chaf1a
T14831 44-50 NN denotes mutant
T14832 51-55 NNS denotes mice
T14833 56-59 CC denotes and
T14834 60-67 NNS denotes embryos
T14835 67-68 . denotes .
T14836 68-156 sentence denotes Using PCR, we amplified two genomic fragments (about 3 kb each) flanking Chaf1a exon 3.
T14837 69-74 VBG denotes Using
T14838 83-92 VBD denotes amplified
T14839 75-78 NN denotes PCR
T14840 78-80 , denotes ,
T14841 80-82 PRP denotes we
T14842 93-96 CD denotes two
T14843 105-114 NNS denotes fragments
T14844 97-104 JJ denotes genomic
T14845 115-116 -LRB- denotes (
T14846 124-126 NN denotes kb
T14847 116-121 IN denotes about
T14848 122-123 CD denotes 3
T14849 127-131 DT denotes each
T14850 131-132 -RRB- denotes )
T14851 133-141 VBG denotes flanking
T14852 142-148 NN denotes Chaf1a
T14853 149-153 NN denotes exon
T14854 154-155 CD denotes 3
T14855 155-156 . denotes .
T14856 156-297 sentence denotes These DNA fragments were assembled by conventional cloning with the neomycin and DT (diphtheria toxin) cassettes, as described in Figure 1A.
T14857 157-162 DT denotes These
T14858 167-176 NNS denotes fragments
T14859 163-166 NN denotes DNA
T14860 182-191 VBN denotes assembled
T14861 177-181 VBD denotes were
T14862 192-194 IN denotes by
T14863 195-207 JJ denotes conventional
T14864 208-215 NN denotes cloning
T14865 216-220 IN denotes with
T14866 221-224 DT denotes the
T14867 260-269 NNS denotes cassettes
T14868 225-233 NN denotes neomycin
T14869 234-237 CC denotes and
T14870 238-240 NN denotes DT
T14871 241-242 -LRB- denotes (
T14872 242-252 NN denotes diphtheria
T14873 253-258 NN denotes toxin
T14874 258-259 -RRB- denotes )
T14875 269-271 , denotes ,
T14876 271-273 IN denotes as
T14877 274-283 VBN denotes described
T14878 284-286 IN denotes in
T14879 287-293 NN denotes Figure
T14880 294-296 NN denotes 1A
T14881 296-297 . denotes .
T14882 297-359 sentence denotes The construct was transfected in ES cells by electroporation.
T14883 298-301 DT denotes The
T14884 302-311 NN denotes construct
T14885 316-327 VBN denotes transfected
T14886 312-315 VBD denotes was
T14887 328-330 IN denotes in
T14888 331-333 NN denotes ES
T14889 334-339 NNS denotes cells
T14890 340-342 IN denotes by
T14891 343-358 NN denotes electroporation
T14892 358-359 . denotes .
T14893 359-506 sentence denotes We identified recombinant ES cells carrying the mutant Chaf1a tm1Ger (abbreviated Chaf1a − in the manuscript) allele by Southern blot (Figure 1A).
T14894 360-362 PRP denotes We
T14895 363-373 VBD denotes identified
T14896 374-385 JJ denotes recombinant
T14897 389-394 NNS denotes cells
T14898 386-388 NN denotes ES
T14899 395-403 VBG denotes carrying
T14900 404-407 DT denotes the
T14901 470-476 NN denotes allele
T14902 408-414 NN denotes mutant
T14903 415-428 NN denotes Chaf1a tm1Ger
T14904 429-430 -LRB- denotes (
T14905 430-441 VBN denotes abbreviated
T14906 442-448 NN denotes Chaf1a
T14907 449-450 SYM denotes −
T14908 451-453 IN denotes in
T14909 454-457 DT denotes the
T14910 458-468 NN denotes manuscript
T14911 468-469 -RRB- denotes )
T14912 477-479 IN denotes by
T14913 480-488 NNP denotes Southern
T14914 489-493 NN denotes blot
T14915 494-495 -LRB- denotes (
T14916 502-504 NN denotes 1A
T14917 495-501 NN denotes Figure
T14918 504-505 -RRB- denotes )
T14919 505-506 . denotes .
T14920 506-593 sentence denotes We derived Chaf1a +/− mice by injecting recombined ES cells into C57BL/6N blastocysts.
T14921 507-509 PRP denotes We
T14922 510-517 VBD denotes derived
T14923 518-524 NN denotes Chaf1a
T14924 529-533 NNS denotes mice
T14925 525-526 SYM denotes +
T14926 526-527 HYPH denotes /
T14927 527-528 SYM denotes −
T14928 534-536 IN denotes by
T14929 537-546 VBG denotes injecting
T14930 547-557 VBN denotes recombined
T14931 561-566 NNS denotes cells
T14932 558-560 NN denotes ES
T14933 567-571 IN denotes into
T14934 572-577 NN denotes C57BL
T14935 578-580 NN denotes 6N
T14936 577-578 HYPH denotes /
T14937 581-592 NNS denotes blastocysts
T14938 592-593 . denotes .
T14939 593-696 sentence denotes E4 embryos obtained from the intercross of Chaf1a +/− mice were genotyped by nested-PCR amplification.
T14940 594-596 NN denotes E4
T14941 597-604 NNS denotes embryos
T14942 658-667 VBN denotes genotyped
T14943 605-613 VBN denotes obtained
T14944 614-618 IN denotes from
T14945 619-622 DT denotes the
T14946 623-633 NN denotes intercross
T14947 634-636 IN denotes of
T14948 637-643 NN denotes Chaf1a
T14949 648-652 NNS denotes mice
T14950 644-645 SYM denotes +
T14951 645-646 HYPH denotes /
T14952 646-647 SYM denotes −
T14953 653-657 VBD denotes were
T14954 668-670 IN denotes by
T14955 671-677 NN denotes nested
T14956 678-681 NN denotes PCR
T14957 677-678 HYPH denotes -
T14958 682-695 NN denotes amplification
T14959 695-696 . denotes .
T14960 696-847 sentence denotes Embryos were collected in a PCR reaction mix containing oligonucleotide primers 1, 2, and 3 (Figure 1A) that amplify the wild-type and mutant alleles.
T14961 697-704 NNS denotes Embryos
T14962 710-719 VBN denotes collected
T14963 705-709 VBD denotes were
T14964 720-722 IN denotes in
T14965 723-724 DT denotes a
T14966 738-741 NN denotes mix
T14967 725-728 NN denotes PCR
T14968 729-737 NN denotes reaction
T14969 742-752 VBG denotes containing
T14970 753-768 NN denotes oligonucleotide
T14971 777-778 CD denotes 1
T14972 769-776 NNS denotes primers
T14973 778-780 , denotes ,
T14974 780-781 CD denotes 2
T14975 781-783 , denotes ,
T14976 783-786 CC denotes and
T14977 787-788 CD denotes 3
T14978 789-790 -LRB- denotes (
T14979 797-799 NN denotes 1A
T14980 790-796 NN denotes Figure
T14981 799-800 -RRB- denotes )
T14982 801-805 WDT denotes that
T14983 806-813 VBP denotes amplify
T14984 814-817 DT denotes the
T14985 839-846 NNS denotes alleles
T14986 818-822 JJ denotes wild
T14987 823-827 NN denotes type
T14988 822-823 HYPH denotes -
T14989 828-831 CC denotes and
T14990 832-838 NN denotes mutant
T14991 846-847 . denotes .
T14992 847-998 sentence denotes After 30 cycles of amplification, 1 μl of each PCR reaction was used in a second round of PCR amplification with a new set of oligonucleotide primers.
T14993 848-853 IN denotes After
T14994 912-916 VBN denotes used
T14995 854-856 CD denotes 30
T14996 857-863 NNS denotes cycles
T14997 864-866 IN denotes of
T14998 867-880 NN denotes amplification
T14999 880-882 , denotes ,
T15000 882-883 CD denotes 1
T15001 884-886 NN denotes μl
T15002 887-889 IN denotes of
T15003 890-894 DT denotes each
T15004 899-907 NN denotes reaction
T15005 895-898 NN denotes PCR
T15006 908-911 VBD denotes was
T15007 917-919 IN denotes in
T15008 920-921 DT denotes a
T15009 929-934 NN denotes round
T15010 922-928 JJ denotes second
T15011 935-937 IN denotes of
T15012 938-941 NN denotes PCR
T15013 942-955 NN denotes amplification
T15014 956-960 IN denotes with
T15015 961-962 DT denotes a
T15016 967-970 NN denotes set
T15017 963-966 JJ denotes new
T15018 971-973 IN denotes of
T15019 974-989 NN denotes oligonucleotide
T15020 990-997 NNS denotes primers
T15021 997-998 . denotes .
T15022 998-1164 sentence denotes After 30 cycles of amplification, PCR reactions were run onto an agarose gel, which revealed the presence of the wild-type (150 bp) and recombinant (200 bp) alleles.
T15023 999-1004 IN denotes After
T15024 1052-1055 VBN denotes run
T15025 1005-1007 CD denotes 30
T15026 1008-1014 NNS denotes cycles
T15027 1015-1017 IN denotes of
T15028 1018-1031 NN denotes amplification
T15029 1031-1033 , denotes ,
T15030 1033-1036 NN denotes PCR
T15031 1037-1046 NNS denotes reactions
T15032 1047-1051 VBD denotes were
T15033 1056-1060 IN denotes onto
T15034 1061-1063 DT denotes an
T15035 1072-1075 NN denotes gel
T15036 1064-1071 NN denotes agarose
T15037 1075-1077 , denotes ,
T15038 1077-1082 WDT denotes which
T15039 1083-1091 VBD denotes revealed
T15040 1092-1095 DT denotes the
T15041 1096-1104 NN denotes presence
T15042 1105-1107 IN denotes of
T15043 1108-1111 DT denotes the
T15044 1156-1163 NNS denotes alleles
T15045 1112-1116 JJ denotes wild
T15046 1117-1121 NN denotes type
T15047 1116-1117 HYPH denotes -
T15048 1122-1123 -LRB- denotes (
T15049 1127-1129 NN denotes bp
T15050 1123-1126 CD denotes 150
T15051 1129-1130 -RRB- denotes )
T15052 1131-1134 CC denotes and
T15053 1135-1146 NN denotes recombinant
T15054 1147-1148 -LRB- denotes (
T15055 1152-1154 NN denotes bp
T15056 1148-1151 CD denotes 200
T15057 1154-1155 -RRB- denotes )
T15058 1163-1164 . denotes .
T15693 1166-1173 NN denotes P150CAF
T15694 1176-1185 NN denotes depletion
T15695 1173-1174 HYPH denotes -
T15696 1174-1175 CD denotes 1
T15697 1186-1188 IN denotes by
T15698 1189-1193 NN denotes RNAi
T15699 1193-1194 . denotes .
T15700 1194-1338 sentence denotes The RNAi plasmid vector that we used in this study contains the mouse H1 promoter and a puromycin selection gene (SB and MG, unpublished data).
T15701 1195-1198 DT denotes The
T15702 1212-1218 NN denotes vector
T15703 1199-1203 NN denotes RNAi
T15704 1204-1211 NN denotes plasmid
T15705 1246-1254 VBZ denotes contains
T15706 1219-1223 WDT denotes that
T15707 1227-1231 VBD denotes used
T15708 1224-1226 PRP denotes we
T15709 1232-1234 IN denotes in
T15710 1235-1239 DT denotes this
T15711 1240-1245 NN denotes study
T15712 1255-1258 DT denotes the
T15713 1268-1276 NN denotes promoter
T15714 1259-1264 NN denotes mouse
T15715 1265-1267 NN denotes H1
T15716 1277-1280 CC denotes and
T15717 1281-1282 DT denotes a
T15718 1303-1307 NN denotes gene
T15719 1283-1292 NN denotes puromycin
T15720 1293-1302 NN denotes selection
T15721 1308-1309 -LRB- denotes (
T15722 1332-1336 NNS denotes data
T15723 1309-1311 NN denotes SB
T15724 1312-1315 CC denotes and
T15725 1316-1318 NN denotes MG
T15726 1318-1320 , denotes ,
T15727 1320-1331 JJ denotes unpublished
T15728 1336-1337 -RRB- denotes )
T15729 1337-1338 . denotes .
T15730 1338-1604 sentence denotes We characterized in detail the properties of this new vector using a GFP target gene and FACS: Extinction of target genes in ES cells is highly efficient in 70% of the cells, of intermediate efficiency in 15%, and inefficient in the remaining 15% of cells analysis.
T15731 1339-1341 PRP denotes We
T15732 1342-1355 VBD denotes characterized
T15733 1473-1475 VBZ denotes is
T15734 1356-1358 IN denotes in
T15735 1359-1365 NN denotes detail
T15736 1366-1369 DT denotes the
T15737 1370-1380 NNS denotes properties
T15738 1381-1383 IN denotes of
T15739 1384-1388 DT denotes this
T15740 1393-1399 NN denotes vector
T15741 1389-1392 JJ denotes new
T15742 1400-1405 VBG denotes using
T15743 1406-1407 DT denotes a
T15744 1419-1423 NN denotes gene
T15745 1408-1411 NN denotes GFP
T15746 1412-1418 NN denotes target
T15747 1424-1427 CC denotes and
T15748 1428-1432 NN denotes FACS
T15749 1432-1434 : denotes :
T15750 1434-1444 NN denotes Extinction
T15751 1445-1447 IN denotes of
T15752 1448-1454 NN denotes target
T15753 1455-1460 NNS denotes genes
T15754 1461-1463 IN denotes in
T15755 1464-1466 NN denotes ES
T15756 1467-1472 NNS denotes cells
T15757 1476-1482 RB denotes highly
T15758 1483-1492 JJ denotes efficient
T15759 1493-1495 IN denotes in
T15760 1496-1498 CD denotes 70
T15761 1498-1499 NN denotes %
T15762 1500-1502 IN denotes of
T15763 1503-1506 DT denotes the
T15764 1507-1512 NNS denotes cells
T15765 1512-1514 , denotes ,
T15766 1514-1516 IN denotes of
T15767 1517-1529 JJ denotes intermediate
T15768 1530-1540 NN denotes efficiency
T15769 1541-1543 IN denotes in
T15770 1544-1546 CD denotes 15
T15771 1546-1547 NN denotes %
T15772 1547-1549 , denotes ,
T15773 1549-1552 CC denotes and
T15774 1553-1564 JJ denotes inefficient
T15775 1565-1567 IN denotes in
T15776 1568-1571 DT denotes the
T15777 1584-1585 NN denotes %
T15778 1572-1581 VBG denotes remaining
T15779 1582-1584 CD denotes 15
T15780 1586-1588 IN denotes of
T15781 1589-1594 NNS denotes cells
T15782 1595-1603 NN denotes analysis
T15783 1603-1604 . denotes .
T15784 1604-1702 sentence denotes The sequence of p150CAF-1 siRNA (short interfering RNA) duplex [16] was cloned into this plasmid.
T15785 1605-1608 DT denotes The
T15786 1609-1617 NN denotes sequence
T15787 1677-1683 VBN denotes cloned
T15788 1618-1620 IN denotes of
T15789 1621-1628 NN denotes p150CAF
T15790 1661-1667 NN denotes duplex
T15791 1628-1629 HYPH denotes -
T15792 1629-1630 CD denotes 1
T15793 1631-1636 NN denotes siRNA
T15794 1637-1638 -LRB- denotes (
T15795 1638-1643 JJ denotes short
T15796 1656-1659 NN denotes RNA
T15797 1644-1655 VBG denotes interfering
T15798 1659-1660 -RRB- denotes )
T15799 1668-1669 -LRB- denotes [
T15800 1669-1671 CD denotes 16
T15801 1671-1672 -RRB- denotes ]
T15802 1673-1676 VBD denotes was
T15803 1684-1688 IN denotes into
T15804 1689-1693 DT denotes this
T15805 1694-1701 NN denotes plasmid
T15806 1701-1702 . denotes .
T15807 1702-1773 sentence denotes The control vector expressed a siRNA that targets GFP RNA degradation.
T15808 1703-1706 DT denotes The
T15809 1715-1721 NN denotes vector
T15810 1707-1714 NN denotes control
T15811 1722-1731 VBD denotes expressed
T15812 1732-1733 DT denotes a
T15813 1734-1739 NN denotes siRNA
T15814 1740-1744 WDT denotes that
T15815 1745-1752 VBZ denotes targets
T15816 1753-1756 NN denotes GFP
T15817 1761-1772 NN denotes degradation
T15818 1757-1760 NN denotes RNA
T15819 1772-1773 . denotes .
T15820 1773-1933 sentence denotes ES cells were transfected by electroporation with the RNA plasmid vector, seeded onto gelatin-coated slides, and cultured for 24 h in the absence of selection.
T15821 1774-1776 NN denotes ES
T15822 1777-1782 NNS denotes cells
T15823 1788-1799 VBN denotes transfected
T15824 1783-1787 VBD denotes were
T15825 1800-1802 IN denotes by
T15826 1803-1818 NN denotes electroporation
T15827 1819-1823 IN denotes with
T15828 1824-1827 DT denotes the
T15829 1840-1846 NN denotes vector
T15830 1828-1831 NN denotes RNA
T15831 1832-1839 NN denotes plasmid
T15832 1846-1848 , denotes ,
T15833 1848-1854 VBN denotes seeded
T15834 1855-1859 IN denotes onto
T15835 1860-1867 NN denotes gelatin
T15836 1868-1874 VBN denotes coated
T15837 1867-1868 HYPH denotes -
T15838 1875-1881 NNS denotes slides
T15839 1881-1883 , denotes ,
T15840 1883-1886 CC denotes and
T15841 1887-1895 VBN denotes cultured
T15842 1896-1899 IN denotes for
T15843 1900-1902 CD denotes 24
T15844 1903-1904 NN denotes h
T15845 1905-1907 IN denotes in
T15846 1908-1911 DT denotes the
T15847 1912-1919 NN denotes absence
T15848 1920-1922 IN denotes of
T15849 1923-1932 NN denotes selection
T15850 1932-1933 . denotes .
T15851 1933-2040 sentence denotes Puromycin (2 μg/ml) was added to the culture medium and cells were cultured for an additional 48-h period.
T15852 1934-1943 NN denotes Puromycin
T15853 1958-1963 VBN denotes added
T15854 1944-1945 -LRB- denotes (
T15855 1947-1949 NN denotes μg
T15856 1945-1946 CD denotes 2
T15857 1949-1950 SYM denotes /
T15858 1950-1952 NN denotes ml
T15859 1952-1953 -RRB- denotes )
T15860 1954-1957 VBD denotes was
T15861 1964-1966 IN denotes to
T15862 1967-1970 DT denotes the
T15863 1979-1985 NN denotes medium
T15864 1971-1978 NN denotes culture
T15865 1986-1989 CC denotes and
T15866 1990-1995 NNS denotes cells
T15867 2001-2009 VBN denotes cultured
T15868 1996-2000 VBD denotes were
T15869 2010-2013 IN denotes for
T15870 2014-2016 DT denotes an
T15871 2033-2039 NN denotes period
T15872 2017-2027 JJ denotes additional
T15873 2028-2030 CD denotes 48
T15874 2031-2032 NN denotes h
T15875 2030-2031 HYPH denotes -
T15876 2039-2040 . denotes .
T15877 2040-2165 sentence denotes Using the p150CAF-1 RNAi vector, IF microscopy quantification reveals a complete CAF-1 depletion in most cells (Figure S1B).
T15878 2041-2046 VBG denotes Using
T15879 2103-2110 VBZ denotes reveals
T15880 2047-2050 DT denotes the
T15881 2066-2072 NN denotes vector
T15882 2051-2058 NN denotes p150CAF
T15883 2058-2059 HYPH denotes -
T15884 2059-2060 CD denotes 1
T15885 2061-2065 NN denotes RNAi
T15886 2072-2074 , denotes ,
T15887 2074-2076 NN denotes IF
T15888 2077-2087 NN denotes microscopy
T15889 2088-2102 NN denotes quantification
T15890 2111-2112 DT denotes a
T15891 2128-2137 NN denotes depletion
T15892 2113-2121 JJ denotes complete
T15893 2122-2125 NN denotes CAF
T15894 2125-2126 HYPH denotes -
T15895 2126-2127 CD denotes 1
T15896 2138-2140 IN denotes in
T15897 2141-2145 JJS denotes most
T15898 2146-2151 NNS denotes cells
T15899 2152-2153 -LRB- denotes (
T15900 2160-2163 NN denotes S1B
T15901 2153-2159 NN denotes Figure
T15902 2163-2164 -RRB- denotes )
T15903 2164-2165 . denotes .
T15904 2165-2310 sentence denotes Western blot analysis (Figure S1A) reveals residual p150CAF-1 expression, as expected from the 15% of cells that do not inhibit the target gene.
T15905 2166-2173 NNP denotes Western
T15906 2174-2178 NN denotes blot
T15907 2179-2187 NN denotes analysis
T15908 2201-2208 VBZ denotes reveals
T15909 2188-2189 -LRB- denotes (
T15910 2196-2199 NN denotes S1A
T15911 2189-2195 NN denotes Figure
T15912 2199-2200 -RRB- denotes )
T15913 2209-2217 JJ denotes residual
T15914 2228-2238 NN denotes expression
T15915 2218-2225 NN denotes p150CAF
T15916 2225-2226 HYPH denotes -
T15917 2226-2227 CD denotes 1
T15918 2238-2240 , denotes ,
T15919 2240-2242 IN denotes as
T15920 2243-2251 VBN denotes expected
T15921 2252-2256 IN denotes from
T15922 2257-2260 DT denotes the
T15923 2263-2264 NN denotes %
T15924 2261-2263 CD denotes 15
T15925 2265-2267 IN denotes of
T15926 2268-2273 NNS denotes cells
T15927 2274-2278 WDT denotes that
T15928 2286-2293 VB denotes inhibit
T15929 2279-2281 VBP denotes do
T15930 2282-2285 RB denotes not
T15931 2294-2297 DT denotes the
T15932 2305-2309 NN denotes gene
T15933 2298-2304 NN denotes target
T15934 2309-2310 . denotes .
T15935 2310-2516 sentence denotes MEFs (passage 3, 90% confluence) were transfected during 4 h with the RNAi plasmid vector using Lipofectamine 2000 (Invitrogene, Carlsbad, California, United States) according to manufacturer's conditions.
T15936 2311-2315 NNS denotes MEFs
T15937 2349-2360 VBN denotes transfected
T15938 2316-2317 -LRB- denotes (
T15939 2317-2324 NN denotes passage
T15940 2325-2326 CD denotes 3
T15941 2326-2328 , denotes ,
T15942 2328-2330 CD denotes 90
T15943 2330-2331 NN denotes %
T15944 2332-2342 NN denotes confluence
T15945 2342-2343 -RRB- denotes )
T15946 2344-2348 VBD denotes were
T15947 2361-2367 IN denotes during
T15948 2368-2369 CD denotes 4
T15949 2370-2371 NN denotes h
T15950 2372-2376 IN denotes with
T15951 2377-2380 DT denotes the
T15952 2394-2400 NN denotes vector
T15953 2381-2385 NN denotes RNAi
T15954 2386-2393 NN denotes plasmid
T15955 2401-2406 VBG denotes using
T15956 2407-2420 NNP denotes Lipofectamine
T15957 2421-2425 CD denotes 2000
T15958 2426-2427 -LRB- denotes (
T15959 2427-2438 NNP denotes Invitrogene
T15960 2438-2440 , denotes ,
T15961 2440-2448 NNP denotes Carlsbad
T15962 2448-2450 , denotes ,
T15963 2450-2460 NNP denotes California
T15964 2460-2462 , denotes ,
T15965 2462-2468 NNP denotes United
T15966 2469-2475 NNP denotes States
T15967 2475-2476 -RRB- denotes )
T15968 2477-2486 VBG denotes according
T15969 2487-2489 IN denotes to
T15970 2490-2502 NN denotes manufacturer
T15971 2505-2515 NNS denotes conditions
T15972 2502-2504 POS denotes 's
T15973 2515-2516 . denotes .
T15974 2516-2616 sentence denotes Cells were trypsinized, plated at 1/3 dilution, and cultured for 48 h in the presence of puromycin.
T15975 2517-2522 NNS denotes Cells
T15976 2528-2539 VBN denotes trypsinized
T15977 2523-2527 VBD denotes were
T15978 2539-2541 , denotes ,
T15979 2541-2547 VBN denotes plated
T15980 2548-2550 IN denotes at
T15981 2551-2552 CD denotes 1
T15982 2553-2554 CD denotes 3
T15983 2552-2553 SYM denotes /
T15984 2555-2563 NN denotes dilution
T15985 2563-2565 , denotes ,
T15986 2565-2568 CC denotes and
T15987 2569-2577 VBN denotes cultured
T15988 2578-2581 IN denotes for
T15989 2582-2584 CD denotes 48
T15990 2585-2586 NN denotes h
T15991 2587-2589 IN denotes in
T15992 2590-2593 DT denotes the
T15993 2594-2602 NN denotes presence
T15994 2603-2605 IN denotes of
T15995 2606-2615 NN denotes puromycin
T15996 2615-2616 . denotes .
T16173 2618-2622 NN denotes Cell
T16174 2623-2628 NNS denotes lines
T16175 2628-2629 . denotes .
T16176 2629-2700 sentence denotes AT-1 ES cells [42] (a gift of M. Vernet) were used for gene targeting.
T16177 2630-2632 NN denotes AT
T16178 2638-2643 NNS denotes cells
T16179 2632-2633 HYPH denotes -
T16180 2633-2634 CD denotes 1
T16181 2635-2637 NN denotes ES
T16182 2676-2680 VBN denotes used
T16183 2644-2645 -LRB- denotes [
T16184 2645-2647 CD denotes 42
T16185 2647-2648 -RRB- denotes ]
T16186 2649-2650 -LRB- denotes (
T16187 2652-2656 NN denotes gift
T16188 2650-2651 DT denotes a
T16189 2657-2659 IN denotes of
T16190 2660-2662 NNP denotes M.
T16191 2663-2669 NNP denotes Vernet
T16192 2669-2670 -RRB- denotes )
T16193 2671-2675 VBD denotes were
T16194 2681-2684 IN denotes for
T16195 2685-2689 NN denotes gene
T16196 2690-2699 NN denotes targeting
T16197 2699-2700 . denotes .
T16198 2700-2749 sentence denotes LTM7 ES cells were used in all RNAi experiments.
T16199 2701-2705 NN denotes LTM7
T16200 2709-2714 NNS denotes cells
T16201 2706-2708 NN denotes ES
T16202 2720-2724 VBN denotes used
T16203 2715-2719 VBD denotes were
T16204 2725-2727 IN denotes in
T16205 2728-2731 DT denotes all
T16206 2737-2748 NNS denotes experiments
T16207 2732-2736 NN denotes RNAi
T16208 2748-2749 . denotes .
T16209 2749-2832 sentence denotes We derived this cell line from (C57BL/6 × 129) F1 females bred with C3H/HeJ males.
T16210 2750-2752 PRP denotes We
T16211 2753-2760 VBD denotes derived
T16212 2761-2765 DT denotes this
T16213 2771-2775 NN denotes line
T16214 2766-2770 NN denotes cell
T16215 2776-2780 IN denotes from
T16216 2781-2782 -LRB- denotes (
T16217 2782-2787 NN denotes C57BL
T16218 2800-2807 NNS denotes females
T16219 2787-2788 HYPH denotes /
T16220 2788-2789 CD denotes 6
T16221 2790-2791 SYM denotes ×
T16222 2792-2795 CD denotes 129
T16223 2795-2796 -RRB- denotes )
T16224 2797-2799 NN denotes F1
T16225 2808-2812 VBN denotes bred
T16226 2813-2817 IN denotes with
T16227 2818-2821 NN denotes C3H
T16228 2822-2825 NN denotes HeJ
T16229 2821-2822 HYPH denotes /
T16230 2826-2831 NNS denotes males
T16231 2831-2832 . denotes .
T16232 2832-2892 sentence denotes LTM7 are XX ES cells, competent for germ line transmission.
T16233 2833-2837 NN denotes LTM7
T16234 2838-2841 VBP denotes are
T16235 2842-2844 NN denotes XX
T16236 2848-2853 NNS denotes cells
T16237 2845-2847 NN denotes ES
T16238 2853-2855 , denotes ,
T16239 2855-2864 JJ denotes competent
T16240 2865-2868 IN denotes for
T16241 2869-2873 NN denotes germ
T16242 2874-2878 NN denotes line
T16243 2879-2891 NN denotes transmission
T16244 2891-2892 . denotes .
T16245 2892-2957 sentence denotes Primary MEFs were derived from E13 embryos as described in [43].
T16246 2893-2900 JJ denotes Primary
T16247 2901-2905 NNS denotes MEFs
T16248 2911-2918 VBN denotes derived
T16249 2906-2910 VBD denotes were
T16250 2919-2923 IN denotes from
T16251 2924-2927 NN denotes E13
T16252 2928-2935 NNS denotes embryos
T16253 2936-2938 IN denotes as
T16254 2939-2948 VBN denotes described
T16255 2949-2951 IN denotes in
T16256 2952-2953 -LRB- denotes [
T16257 2953-2955 CD denotes 43
T16258 2955-2956 -RRB- denotes ]
T16259 2956-2957 . denotes .
T16587 2959-2977 NN denotes Immunofluorescence
T16588 2977-2978 . denotes .
T16589 2978-3103 sentence denotes Cells were fixed for 20 min in PBS with 4% paraformaldehyde, and immunodetection was performed as previously described [44].
T16590 2979-2984 NNS denotes Cells
T16591 2990-2995 VBN denotes fixed
T16592 2985-2989 VBD denotes were
T16593 2996-2999 IN denotes for
T16594 3000-3002 CD denotes 20
T16595 3003-3006 NN denotes min
T16596 3007-3009 IN denotes in
T16597 3010-3013 NN denotes PBS
T16598 3014-3018 IN denotes with
T16599 3019-3020 CD denotes 4
T16600 3020-3021 NN denotes %
T16601 3022-3038 NN denotes paraformaldehyde
T16602 3038-3040 , denotes ,
T16603 3040-3043 CC denotes and
T16604 3044-3059 NN denotes immunodetection
T16605 3064-3073 VBN denotes performed
T16606 3060-3063 VBD denotes was
T16607 3074-3076 IN denotes as
T16608 3088-3097 VBN denotes described
T16609 3077-3087 RB denotes previously
T16610 3098-3099 -LRB- denotes [
T16611 3099-3101 CD denotes 44
T16612 3101-3102 -RRB- denotes ]
T16613 3102-3103 . denotes .
T16614 3103-3282 sentence denotes E4 embryos were collected and treated with tyrode acid to remove the zona pellucida, deposited onto microscope slides, and processed for immunostaining as described for ES cells.
T16615 3104-3106 NN denotes E4
T16616 3107-3114 NNS denotes embryos
T16617 3120-3129 VBN denotes collected
T16618 3115-3119 VBD denotes were
T16619 3130-3133 CC denotes and
T16620 3134-3141 VBN denotes treated
T16621 3142-3146 IN denotes with
T16622 3147-3153 NN denotes tyrode
T16623 3154-3158 NN denotes acid
T16624 3159-3161 TO denotes to
T16625 3162-3168 VB denotes remove
T16626 3169-3172 DT denotes the
T16627 3178-3187 NN denotes pellucida
T16628 3173-3177 NN denotes zona
T16629 3187-3189 , denotes ,
T16630 3189-3198 VBN denotes deposited
T16631 3199-3203 IN denotes onto
T16632 3204-3214 NN denotes microscope
T16633 3215-3221 NNS denotes slides
T16634 3221-3223 , denotes ,
T16635 3223-3226 CC denotes and
T16636 3227-3236 VBN denotes processed
T16637 3237-3240 IN denotes for
T16638 3241-3255 NN denotes immunostaining
T16639 3256-3258 IN denotes as
T16640 3259-3268 VBN denotes described
T16641 3269-3272 IN denotes for
T16642 3273-3275 NN denotes ES
T16643 3276-3281 NNS denotes cells
T16644 3281-3282 . denotes .
T16645 3282-3566 sentence denotes Antibodies anti-HP1α (2HP1H5, Euromedex, France), anti-H4K20me3 (Abcam, Cambridge, United Kingdom), anti-BrdU (DakoCytomation, Glostrup, Denmark), anti-PCNA (DakoCytomation), and anti-PML (Upstate Biotechnology, Lake Placid, New York, United States) were all used at 1/1000 dilution.
T16646 3283-3293 NNS denotes Antibodies
T16647 3542-3546 VBN denotes used
T16648 3294-3303 JJ denotes anti-HP1α
T16649 3304-3305 -LRB- denotes (
T16650 3305-3311 NN denotes 2HP1H5
T16651 3311-3313 , denotes ,
T16652 3313-3322 NNP denotes Euromedex
T16653 3322-3324 , denotes ,
T16654 3324-3330 NNP denotes France
T16655 3330-3331 -RRB- denotes )
T16656 3331-3333 , denotes ,
T16657 3333-3346 JJ denotes anti-H4K20me3
T16658 3347-3348 -LRB- denotes (
T16659 3348-3353 NNP denotes Abcam
T16660 3353-3355 , denotes ,
T16661 3355-3364 NNP denotes Cambridge
T16662 3364-3366 , denotes ,
T16663 3366-3372 NNP denotes United
T16664 3373-3380 NNP denotes Kingdom
T16665 3380-3381 -RRB- denotes )
T16666 3381-3383 , denotes ,
T16667 3383-3392 JJ denotes anti-BrdU
T16668 3393-3394 -LRB- denotes (
T16669 3394-3408 NNP denotes DakoCytomation
T16670 3408-3410 , denotes ,
T16671 3410-3418 NNP denotes Glostrup
T16672 3418-3420 , denotes ,
T16673 3420-3427 NNP denotes Denmark
T16674 3427-3428 -RRB- denotes )
T16675 3428-3430 , denotes ,
T16676 3430-3439 JJ denotes anti-PCNA
T16677 3440-3441 -LRB- denotes (
T16678 3441-3455 NNP denotes DakoCytomation
T16679 3455-3456 -RRB- denotes )
T16680 3456-3458 , denotes ,
T16681 3458-3461 CC denotes and
T16682 3462-3470 JJ denotes anti-PML
T16683 3471-3472 -LRB- denotes (
T16684 3480-3493 NNP denotes Biotechnology
T16685 3472-3479 NNP denotes Upstate
T16686 3493-3495 , denotes ,
T16687 3495-3499 NNP denotes Lake
T16688 3500-3506 NNP denotes Placid
T16689 3506-3508 , denotes ,
T16690 3508-3511 NNP denotes New
T16691 3512-3516 NNP denotes York
T16692 3516-3518 , denotes ,
T16693 3518-3524 NNP denotes United
T16694 3525-3531 NNP denotes States
T16695 3531-3532 -RRB- denotes )
T16696 3533-3537 VBD denotes were
T16697 3538-3541 RB denotes all
T16698 3547-3549 IN denotes at
T16699 3550-3551 CD denotes 1
T16700 3557-3565 NN denotes dilution
T16701 3551-3552 SYM denotes /
T16702 3552-3556 CD denotes 1000
T16703 3565-3566 . denotes .
T16704 3566-3687 sentence denotes Antibodies anti-mouse p150CAF1 [16] and anti-H3K9me3 (Upstate Biotechnology) were used at 1/250 and 1/500, respectively.
T16705 3567-3577 NNS denotes Antibodies
T16706 3589-3597 NN denotes p150CAF1
T16707 3578-3588 JJ denotes anti-mouse
T16708 3649-3653 VBN denotes used
T16709 3598-3599 -LRB- denotes [
T16710 3599-3601 CD denotes 16
T16711 3601-3602 -RRB- denotes ]
T16712 3603-3606 CC denotes and
T16713 3607-3619 NN denotes anti-H3K9me3
T16714 3620-3621 -LRB- denotes (
T16715 3629-3642 NNP denotes Biotechnology
T16716 3621-3628 NNP denotes Upstate
T16717 3642-3643 -RRB- denotes )
T16718 3644-3648 VBD denotes were
T16719 3654-3656 IN denotes at
T16720 3657-3658 CD denotes 1
T16721 3658-3659 SYM denotes /
T16722 3659-3662 CD denotes 250
T16723 3663-3666 CC denotes and
T16724 3667-3668 CD denotes 1
T16725 3668-3669 SYM denotes /
T16726 3669-3672 CD denotes 500
T16727 3672-3674 , denotes ,
T16728 3674-3686 RB denotes respectively
T16729 3686-3687 . denotes .
T16730 3687-3789 sentence denotes All secondary antibodies were purchased from Molecular Probes (Sunnyvale, California, United States).
T16731 3688-3691 DT denotes All
T16732 3702-3712 NNS denotes antibodies
T16733 3692-3701 JJ denotes secondary
T16734 3718-3727 VBN denotes purchased
T16735 3713-3717 VBD denotes were
T16736 3728-3732 IN denotes from
T16737 3733-3742 NNP denotes Molecular
T16738 3743-3749 NNP denotes Probes
T16739 3750-3751 -LRB- denotes (
T16740 3751-3760 NNP denotes Sunnyvale
T16741 3760-3762 , denotes ,
T16742 3762-3772 NNP denotes California
T16743 3772-3774 , denotes ,
T16744 3774-3780 NNP denotes United
T16745 3781-3787 NNP denotes States
T16746 3787-3788 -RRB- denotes )
T16747 3788-3789 . denotes .
T16924 3791-3798 NNP denotes Western
T16925 3799-3803 NN denotes blot
T16926 3804-3812 NN denotes analysis
T16927 3812-3813 . denotes .
T16928 3813-3892 sentence denotes Cells were lysed in Laemli buffer and run onto a 4%–12% SDS PAGE gradient gel.
T16929 3814-3819 NNS denotes Cells
T16930 3825-3830 VBN denotes lysed
T16931 3820-3824 VBD denotes were
T16932 3831-3833 IN denotes in
T16933 3834-3840 NNP denotes Laemli
T16934 3841-3847 NN denotes buffer
T16935 3848-3851 CC denotes and
T16936 3852-3855 VBP denotes run
T16937 3856-3860 IN denotes onto
T16938 3861-3862 DT denotes a
T16939 3888-3891 NN denotes gel
T16940 3863-3864 CD denotes 4
T16941 3864-3865 NN denotes %
T16942 3865-3866 SYM denotes –
T16943 3868-3869 NN denotes %
T16944 3866-3868 CD denotes 12
T16945 3870-3873 NN denotes SDS
T16946 3874-3878 NN denotes PAGE
T16947 3879-3887 NN denotes gradient
T16948 3891-3892 . denotes .
T16949 3892-4068 sentence denotes We used antibodies against p150CAF-1 ([16]; dilution 1/500), HP1α (2G9, Euromedex; 1/500), Histone H3 (Abcam; 1/500), H3K9me3 (Upstate; 1/500) and β-actin (Upstate; 1/40 000).
T16950 3893-3895 PRP denotes We
T16951 3896-3900 VBD denotes used
T16952 3901-3911 NNS denotes antibodies
T16953 3912-3919 IN denotes against
T16954 3920-3927 NN denotes p150CAF
T16955 3927-3928 HYPH denotes -
T16956 3928-3929 CD denotes 1
T16957 3930-3931 -LRB- denotes (
T16958 3937-3945 NN denotes dilution
T16959 3931-3932 -LRB- denotes [
T16960 3932-3934 CD denotes 16
T16961 3934-3935 -RRB- denotes ]
T16962 3935-3936 : denotes ;
T16963 3946-3947 CD denotes 1
T16964 3947-3948 SYM denotes /
T16965 3948-3951 CD denotes 500
T16966 3951-3952 -RRB- denotes )
T16967 3952-3954 , denotes ,
T16968 3954-3958 NN denotes HP1α
T16969 3959-3960 -LRB- denotes (
T16970 3976-3977 CD denotes 1
T16971 3960-3963 NN denotes 2G9
T16972 3963-3965 , denotes ,
T16973 3965-3974 NNP denotes Euromedex
T16974 3974-3975 : denotes ;
T16975 3977-3978 SYM denotes /
T16976 3978-3981 CD denotes 500
T16977 3981-3982 -RRB- denotes )
T16978 3982-3984 , denotes ,
T16979 3984-3991 NN denotes Histone
T16980 3992-3994 NN denotes H3
T16981 3995-3996 -LRB- denotes (
T16982 4003-4004 CD denotes 1
T16983 3996-4001 NN denotes Abcam
T16984 4001-4002 : denotes ;
T16985 4004-4005 SYM denotes /
T16986 4005-4008 CD denotes 500
T16987 4008-4009 -RRB- denotes )
T16988 4009-4011 , denotes ,
T16989 4011-4018 NN denotes H3K9me3
T16990 4019-4020 -LRB- denotes (
T16991 4029-4030 CD denotes 1
T16992 4020-4027 NNP denotes Upstate
T16993 4027-4028 : denotes ;
T16994 4030-4031 SYM denotes /
T16995 4031-4034 CD denotes 500
T16996 4034-4035 -RRB- denotes )
T16997 4036-4039 CC denotes and
T16998 4040-4041 NN denotes β
T16999 4042-4047 NN denotes actin
T17000 4041-4042 HYPH denotes -
T17001 4048-4049 -LRB- denotes (
T17002 4058-4059 CD denotes 1
T17003 4049-4056 NNP denotes Upstate
T17004 4056-4057 : denotes ;
T17005 4059-4060 SYM denotes /
T17006 4063-4066 CD denotes 000
T17007 4060-4062 CD denotes 40
T17008 4066-4067 -RRB- denotes )
T17009 4067-4068 . denotes .
T17171 4070-4073 NN denotes DNA
T17172 4074-4078 NN denotes FISH
T17173 4078-4079 . denotes .
T17174 4079-4118 sentence denotes Probes were described previously [20].
T17175 4080-4086 NNS denotes Probes
T17176 4092-4101 VBN denotes described
T17177 4087-4091 VBD denotes were
T17178 4102-4112 RB denotes previously
T17179 4113-4114 -LRB- denotes [
T17180 4114-4116 CD denotes 20
T17181 4116-4117 -RRB- denotes ]
T17182 4117-4118 . denotes .
T17183 4118-4280 sentence denotes Biotin-16-dUTP or Digoxigenin-11-dUTP (Roche, Basel, Switzerland) labeled probes were generated by nick translation (Roche) and FISH performed as described [20].
T17184 4119-4125 NN denotes Biotin
T17185 4129-4133 NN denotes dUTP
T17186 4125-4126 HYPH denotes -
T17187 4126-4128 CD denotes 16
T17188 4128-4129 HYPH denotes -
T17189 4185-4192 VBN denotes labeled
T17190 4134-4136 CC denotes or
T17191 4137-4148 NN denotes Digoxigenin
T17192 4152-4156 NN denotes dUTP
T17193 4148-4149 HYPH denotes -
T17194 4149-4151 CD denotes 11
T17195 4151-4152 HYPH denotes -
T17196 4157-4158 -LRB- denotes (
T17197 4158-4163 NNP denotes Roche
T17198 4163-4165 , denotes ,
T17199 4165-4170 NNP denotes Basel
T17200 4170-4172 , denotes ,
T17201 4172-4183 NNP denotes Switzerland
T17202 4183-4184 -RRB- denotes )
T17203 4193-4199 NNS denotes probes
T17204 4205-4214 VBN denotes generated
T17205 4200-4204 VBD denotes were
T17206 4215-4217 IN denotes by
T17207 4218-4222 NN denotes nick
T17208 4223-4234 NN denotes translation
T17209 4235-4236 -LRB- denotes (
T17210 4236-4241 NNP denotes Roche
T17211 4241-4242 -RRB- denotes )
T17212 4243-4246 CC denotes and
T17213 4247-4251 NN denotes FISH
T17214 4252-4261 VBN denotes performed
T17215 4262-4264 IN denotes as
T17216 4265-4274 VBN denotes described
T17217 4275-4276 -LRB- denotes [
T17218 4276-4278 CD denotes 20
T17219 4278-4279 -RRB- denotes ]
T17220 4279-4280 . denotes .
T17221 4280-4474 sentence denotes Image acquisition was performed with the Deltavision RT microscope (100×, 1.4 NA objective), images were deconvoluted, and fluorescence profiles measured along an arbitrary line using SoftWorx.
T17222 4281-4286 NN denotes Image
T17223 4287-4298 NN denotes acquisition
T17224 4303-4312 VBN denotes performed
T17225 4299-4302 VBD denotes was
T17226 4313-4317 IN denotes with
T17227 4318-4321 DT denotes the
T17228 4337-4347 NN denotes microscope
T17229 4322-4333 NNP denotes Deltavision
T17230 4334-4336 NN denotes RT
T17231 4348-4349 -LRB- denotes (
T17232 4362-4371 NN denotes objective
T17233 4349-4352 CD denotes 100
T17234 4352-4353 SYM denotes ×
T17235 4353-4355 , denotes ,
T17236 4355-4358 CD denotes 1.4
T17237 4359-4361 NN denotes NA
T17238 4371-4372 -RRB- denotes )
T17239 4372-4374 , denotes ,
T17240 4374-4380 NNS denotes images
T17241 4386-4398 VBN denotes deconvoluted
T17242 4381-4385 VBD denotes were
T17243 4398-4400 , denotes ,
T17244 4400-4403 CC denotes and
T17245 4404-4416 NN denotes fluorescence
T17246 4417-4425 NNS denotes profiles
T17247 4426-4434 VBN denotes measured
T17248 4435-4440 IN denotes along
T17249 4441-4443 DT denotes an
T17250 4454-4458 NN denotes line
T17251 4444-4453 JJ denotes arbitrary
T17252 4459-4464 VBG denotes using
T17253 4465-4473 NNP denotes SoftWorx
T17254 4473-4474 . denotes .
T17929 4476-4482 NNS denotes Nuclei
T17930 4483-4494 NN denotes preparation
T17931 4494-4496 , denotes ,
T17932 4496-4504 NN denotes nuclease
T17933 4505-4514 NN denotes digestion
T17934 4514-4516 , denotes ,
T17935 4516-4519 CC denotes and
T17936 4520-4531 JJ denotes biochemical
T17937 4532-4540 NN denotes analysis
T17938 4541-4543 IN denotes of
T17939 4544-4553 NN denotes chromatin
T17940 4553-4554 . denotes .
T17941 4554-4760 sentence denotes ES cells were incubated on ice for 10 min in buffer 1 (15 mM Tris-HCl [pH 7.5], 0.3 M sucrose, 60 mM KCl, 15 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA) with 0.15% IGEPAL (Sigma, St. Louis, Missouri, United States).
T17942 4555-4557 NN denotes ES
T17943 4558-4563 NNS denotes cells
T17944 4569-4578 VBN denotes incubated
T17945 4564-4568 VBD denotes were
T17946 4579-4581 IN denotes on
T17947 4582-4585 NN denotes ice
T17948 4586-4589 IN denotes for
T17949 4590-4592 CD denotes 10
T17950 4593-4596 NN denotes min
T17951 4597-4599 IN denotes in
T17952 4600-4606 NN denotes buffer
T17953 4607-4608 CD denotes 1
T17954 4609-4610 -LRB- denotes (
T17955 4621-4624 NN denotes HCl
T17956 4610-4612 CD denotes 15
T17957 4613-4615 NN denotes mM
T17958 4616-4620 NN denotes Tris
T17959 4620-4621 HYPH denotes -
T17960 4625-4626 -LRB- denotes [
T17961 4626-4628 NN denotes pH
T17962 4629-4632 CD denotes 7.5
T17963 4632-4633 -RRB- denotes ]
T17964 4633-4635 , denotes ,
T17965 4635-4638 CD denotes 0.3
T17966 4639-4640 NN denotes M
T17967 4641-4648 NN denotes sucrose
T17968 4648-4650 , denotes ,
T17969 4650-4652 CD denotes 60
T17970 4653-4655 NN denotes mM
T17971 4656-4659 NN denotes KCl
T17972 4659-4661 , denotes ,
T17973 4661-4663 CD denotes 15
T17974 4664-4666 NN denotes mM
T17975 4667-4671 NN denotes NaCl
T17976 4671-4673 , denotes ,
T17977 4673-4674 CD denotes 5
T17978 4675-4677 NN denotes mM
T17979 4678-4683 NN denotes MgCl2
T17980 4683-4685 , denotes ,
T17981 4685-4688 CD denotes 0.1
T17982 4689-4691 NN denotes mM
T17983 4692-4696 NN denotes EGTA
T17984 4696-4697 -RRB- denotes )
T17985 4698-4702 IN denotes with
T17986 4703-4707 CD denotes 0.15
T17987 4707-4708 NN denotes %
T17988 4709-4715 NN denotes IGEPAL
T17989 4716-4717 -LRB- denotes (
T17990 4717-4722 NNP denotes Sigma
T17991 4722-4724 , denotes ,
T17992 4724-4727 NNP denotes St.
T17993 4728-4733 NNP denotes Louis
T17994 4733-4735 , denotes ,
T17995 4735-4743 NNP denotes Missouri
T17996 4743-4745 , denotes ,
T17997 4745-4751 NNP denotes United
T17998 4752-4758 NNP denotes States
T17999 4758-4759 -RRB- denotes )
T18000 4759-4760 . denotes .
T18001 4760-4996 sentence denotes Nuclei were purified by centrifugation (10,000 × g for 30 min, at 4 °C) on sucrose cushions (buffer 1 with 1.2 M sucrose) and resuspended in nuclease buffer (50 mM Tris-HCl [pH 7.5], 20 mM NaCl, 0.32 M sucrose, 4 mM MgCl2, 1 mM CaCl2).
T18002 4761-4767 NNS denotes Nuclei
T18003 4773-4781 VBN denotes purified
T18004 4768-4772 VBD denotes were
T18005 4782-4784 IN denotes by
T18006 4785-4799 NN denotes centrifugation
T18007 4800-4801 -LRB- denotes (
T18008 4810-4811 NN denotes g
T18009 4801-4807 CD denotes 10,000
T18010 4808-4809 SYM denotes ×
T18011 4812-4815 IN denotes for
T18012 4816-4818 CD denotes 30
T18013 4819-4822 NN denotes min
T18014 4822-4824 , denotes ,
T18015 4824-4826 IN denotes at
T18016 4827-4828 CD denotes 4
T18017 4829-4831 NN denotes °C
T18018 4831-4832 -RRB- denotes )
T18019 4833-4835 IN denotes on
T18020 4836-4843 NN denotes sucrose
T18021 4844-4852 NNS denotes cushions
T18022 4853-4854 -LRB- denotes (
T18023 4854-4860 NN denotes buffer
T18024 4861-4862 CD denotes 1
T18025 4863-4867 IN denotes with
T18026 4868-4871 CD denotes 1.2
T18027 4872-4873 NN denotes M
T18028 4874-4881 NN denotes sucrose
T18029 4881-4882 -RRB- denotes )
T18030 4883-4886 CC denotes and
T18031 4887-4898 VBN denotes resuspended
T18032 4899-4901 IN denotes in
T18033 4902-4910 NN denotes nuclease
T18034 4911-4917 NN denotes buffer
T18035 4918-4919 -LRB- denotes (
T18036 4930-4933 NN denotes HCl
T18037 4919-4921 CD denotes 50
T18038 4922-4924 NN denotes mM
T18039 4925-4929 NN denotes Tris
T18040 4929-4930 HYPH denotes -
T18041 4934-4935 -LRB- denotes [
T18042 4935-4937 NN denotes pH
T18043 4938-4941 CD denotes 7.5
T18044 4941-4942 -RRB- denotes ]
T18045 4942-4944 , denotes ,
T18046 4944-4946 CD denotes 20
T18047 4947-4949 NN denotes mM
T18048 4950-4954 NN denotes NaCl
T18049 4954-4956 , denotes ,
T18050 4956-4960 CD denotes 0.32
T18051 4961-4962 NN denotes M
T18052 4963-4970 NN denotes sucrose
T18053 4970-4972 , denotes ,
T18054 4972-4973 CD denotes 4
T18055 4974-4976 NN denotes mM
T18056 4977-4982 NN denotes MgCl2
T18057 4982-4984 , denotes ,
T18058 4984-4985 CD denotes 1
T18059 4986-4988 NN denotes mM
T18060 4989-4994 NN denotes CaCl2
T18061 4994-4995 -RRB- denotes )
T18062 4995-4996 . denotes .
T18063 4996-5111 sentence denotes About 2.106 nuclei were incubated with increasing quantities of MNase (0.04–1.6 units) or DNase I (0.25–16 units).
T18064 4997-5002 IN denotes About
T18065 5003-5008 CD denotes 2.106
T18066 5009-5015 NNS denotes nuclei
T18067 5021-5030 VBN denotes incubated
T18068 5016-5020 VBD denotes were
T18069 5031-5035 IN denotes with
T18070 5036-5046 VBG denotes increasing
T18071 5047-5057 NNS denotes quantities
T18072 5058-5060 IN denotes of
T18073 5061-5066 NN denotes MNase
T18074 5067-5068 -LRB- denotes (
T18075 5077-5082 NNS denotes units
T18076 5068-5072 CD denotes 0.04
T18077 5073-5076 CD denotes 1.6
T18078 5072-5073 SYM denotes –
T18079 5082-5083 -RRB- denotes )
T18080 5084-5086 CC denotes or
T18081 5087-5092 NN denotes DNase
T18082 5093-5094 CD denotes I
T18083 5095-5096 -LRB- denotes (
T18084 5104-5109 NNS denotes units
T18085 5096-5100 CD denotes 0.25
T18086 5101-5103 CD denotes 16
T18087 5100-5101 SYM denotes –
T18088 5109-5110 -RRB- denotes )
T18089 5110-5111 . denotes .
T18090 5111-5179 sentence denotes Digestion time at 37 °C was 10 min for MNase and 2 min for DNase I.
T18091 5112-5121 NN denotes Digestion
T18092 5122-5126 NN denotes time
T18093 5136-5139 VBD denotes was
T18094 5127-5129 IN denotes at
T18095 5130-5132 CD denotes 37
T18096 5133-5135 NN denotes °C
T18097 5140-5142 CD denotes 10
T18098 5143-5146 NN denotes min
T18099 5147-5150 IN denotes for
T18100 5151-5156 NN denotes MNase
T18101 5157-5160 CC denotes and
T18102 5161-5162 CD denotes 2
T18103 5163-5166 NN denotes min
T18104 5167-5170 IN denotes for
T18105 5171-5176 NN denotes DNase
T18106 5177-5178 CD denotes I
T18107 5178-5179 . denotes .
T18108 5179-5242 sentence denotes Digestions were stopped by adding SDS to 1% and EDTA to 50 mM.
T18109 5180-5190 NNS denotes Digestions
T18110 5196-5203 VBN denotes stopped
T18111 5191-5195 VBD denotes were
T18112 5204-5206 IN denotes by
T18113 5207-5213 VBG denotes adding
T18114 5214-5217 NN denotes SDS
T18115 5218-5220 IN denotes to
T18116 5221-5222 CD denotes 1
T18117 5222-5223 NN denotes %
T18118 5224-5227 CC denotes and
T18119 5228-5232 NN denotes EDTA
T18120 5233-5235 IN denotes to
T18121 5236-5238 CD denotes 50
T18122 5239-5241 NN denotes mM
T18123 5241-5242 . denotes .
T18124 5242-5359 sentence denotes DNAs were prepared by proteinase K digestion followed by phenol-chloroform extraction and isopropanol precipitation.
T18125 5243-5247 NNS denotes DNAs
T18126 5253-5261 VBN denotes prepared
T18127 5248-5252 VBD denotes were
T18128 5262-5264 IN denotes by
T18129 5265-5275 NN denotes proteinase
T18130 5276-5277 NN denotes K
T18131 5278-5287 NN denotes digestion
T18132 5288-5296 VBN denotes followed
T18133 5297-5299 IN denotes by
T18134 5300-5306 NN denotes phenol
T18135 5307-5317 NN denotes chloroform
T18136 5306-5307 HYPH denotes -
T18137 5318-5328 NN denotes extraction
T18138 5329-5332 CC denotes and
T18139 5333-5344 NN denotes isopropanol
T18140 5345-5358 NN denotes precipitation
T18141 5358-5359 . denotes .
T18142 5359-5662 sentence denotes To test the association of histone H3 with chromatin in control and p150-depleted cells, isolated nuclei were incubated on ice for 30 min in buffer 2 (50 mM Hepes [pH7.9], 20% Glycerol, 3 mM MgCl2, 0.1% IGEPAL, 0.5 mM DTT, 0.5 mM PMSF) supplemented with either 0.1 M, 0.3 M, 0.45 M, 0.7 M, or 1 M NaCl.
T18143 5360-5362 TO denotes To
T18144 5363-5367 VB denotes test
T18145 5470-5479 VBN denotes incubated
T18146 5368-5371 DT denotes the
T18147 5372-5383 NN denotes association
T18148 5384-5386 IN denotes of
T18149 5387-5394 NN denotes histone
T18150 5395-5397 NN denotes H3
T18151 5398-5402 IN denotes with
T18152 5403-5412 NN denotes chromatin
T18153 5413-5415 IN denotes in
T18154 5416-5423 NN denotes control
T18155 5442-5447 NNS denotes cells
T18156 5424-5427 CC denotes and
T18157 5428-5432 NN denotes p150
T18158 5433-5441 VBN denotes depleted
T18159 5432-5433 HYPH denotes -
T18160 5447-5449 , denotes ,
T18161 5449-5457 VBN denotes isolated
T18162 5458-5464 NNS denotes nuclei
T18163 5465-5469 VBD denotes were
T18164 5480-5482 IN denotes on
T18165 5483-5486 NN denotes ice
T18166 5487-5490 IN denotes for
T18167 5491-5493 CD denotes 30
T18168 5494-5497 NN denotes min
T18169 5498-5500 IN denotes in
T18170 5501-5507 NN denotes buffer
T18171 5508-5509 CD denotes 2
T18172 5510-5511 -LRB- denotes (
T18173 5517-5522 NN denotes Hepes
T18174 5511-5513 CD denotes 50
T18175 5514-5516 NN denotes mM
T18176 5523-5524 -LRB- denotes [
T18177 5524-5529 NN denotes pH7.9
T18178 5529-5530 -RRB- denotes ]
T18179 5530-5532 , denotes ,
T18180 5532-5534 CD denotes 20
T18181 5534-5535 NN denotes %
T18182 5536-5544 NN denotes Glycerol
T18183 5544-5546 , denotes ,
T18184 5546-5547 CD denotes 3
T18185 5548-5550 NN denotes mM
T18186 5551-5556 NN denotes MgCl2
T18187 5556-5558 , denotes ,
T18188 5558-5561 CD denotes 0.1
T18189 5561-5562 NN denotes %
T18190 5563-5569 NN denotes IGEPAL
T18191 5569-5571 , denotes ,
T18192 5571-5574 CD denotes 0.5
T18193 5575-5577 NN denotes mM
T18194 5578-5581 NN denotes DTT
T18195 5581-5583 , denotes ,
T18196 5583-5586 CD denotes 0.5
T18197 5587-5589 NN denotes mM
T18198 5590-5594 NN denotes PMSF
T18199 5594-5595 -RRB- denotes )
T18200 5596-5608 VBN denotes supplemented
T18201 5609-5613 IN denotes with
T18202 5614-5620 CC denotes either
T18203 5625-5626 NN denotes M
T18204 5621-5624 CD denotes 0.1
T18205 5657-5661 NN denotes NaCl
T18206 5626-5628 , denotes ,
T18207 5628-5631 CD denotes 0.3
T18208 5632-5633 NN denotes M
T18209 5633-5635 , denotes ,
T18210 5635-5639 CD denotes 0.45
T18211 5640-5641 NN denotes M
T18212 5641-5643 , denotes ,
T18213 5643-5646 CD denotes 0.7
T18214 5647-5648 NN denotes M
T18215 5648-5650 , denotes ,
T18216 5650-5652 CC denotes or
T18217 5653-5654 CD denotes 1
T18218 5655-5656 NN denotes M
T18219 5661-5662 . denotes .
T18220 5662-5786 sentence denotes After centrifugation at 15,000 g, pellets and supernants were analyzed by Western blot using a histone H3 antibody (Abcam).
T18221 5663-5668 IN denotes After
T18222 5725-5733 VBN denotes analyzed
T18223 5669-5683 NN denotes centrifugation
T18224 5684-5686 IN denotes at
T18225 5687-5693 CD denotes 15,000
T18226 5694-5695 NN denotes g
T18227 5695-5697 , denotes ,
T18228 5697-5704 NNS denotes pellets
T18229 5705-5708 CC denotes and
T18230 5709-5719 NNS denotes supernants
T18231 5720-5724 VBD denotes were
T18232 5734-5736 IN denotes by
T18233 5737-5744 NNP denotes Western
T18234 5745-5749 NN denotes blot
T18235 5750-5755 VBG denotes using
T18236 5756-5757 DT denotes a
T18237 5769-5777 NN denotes antibody
T18238 5758-5765 NN denotes histone
T18239 5766-5768 NN denotes H3
T18240 5778-5779 -LRB- denotes (
T18241 5779-5784 NNP denotes Abcam
T18242 5784-5785 -RRB- denotes )
T18243 5785-5786 . denotes .
T18631 5788-5792 NN denotes ChIP
T18632 5792-5793 . denotes .
T18633 5793-5887 sentence denotes We prepared native chromatin fragments of two to six nucleosomes in length as described [28].
T18634 5794-5796 PRP denotes We
T18635 5797-5805 VBD denotes prepared
T18636 5806-5812 JJ denotes native
T18637 5823-5832 NNS denotes fragments
T18638 5813-5822 NN denotes chromatin
T18639 5833-5835 IN denotes of
T18640 5836-5839 CD denotes two
T18641 5843-5846 CD denotes six
T18642 5840-5842 IN denotes to
T18643 5847-5858 NNS denotes nucleosomes
T18644 5859-5861 IN denotes in
T18645 5862-5868 NN denotes length
T18646 5869-5871 IN denotes as
T18647 5872-5881 VBN denotes described
T18648 5882-5883 -LRB- denotes [
T18649 5883-5885 CD denotes 28
T18650 5885-5886 -RRB- denotes ]
T18651 5886-5887 . denotes .
T18652 5887-5967 sentence denotes 5 μg of chromatin were incubated overnight with 10 μl of commercial antibodies.
T18653 5888-5889 CD denotes 5
T18654 5890-5892 NN denotes μg
T18655 5911-5920 VBN denotes incubated
T18656 5893-5895 IN denotes of
T18657 5896-5905 NN denotes chromatin
T18658 5906-5910 VBD denotes were
T18659 5921-5930 RB denotes overnight
T18660 5931-5935 IN denotes with
T18661 5936-5938 CD denotes 10
T18662 5939-5941 NN denotes μl
T18663 5942-5944 IN denotes of
T18664 5945-5955 JJ denotes commercial
T18665 5956-5966 NNS denotes antibodies
T18666 5966-5967 . denotes .
T18667 5967-6122 sentence denotes After incubation with protein-G sepharose and three washes, immunoprecipitated DNA was purified, sized onto an agarose gel, and analyzed by Southern blot.
T18668 5968-5973 IN denotes After
T18669 6055-6063 VBN denotes purified
T18670 5974-5984 NN denotes incubation
T18671 5985-5989 IN denotes with
T18672 5990-5997 NN denotes protein
T18673 5998-5999 NN denotes G
T18674 5997-5998 HYPH denotes -
T18675 6000-6009 NN denotes sepharose
T18676 6010-6013 CC denotes and
T18677 6014-6019 CD denotes three
T18678 6020-6026 NNS denotes washes
T18679 6026-6028 , denotes ,
T18680 6028-6046 VBN denotes immunoprecipitated
T18681 6047-6050 NN denotes DNA
T18682 6051-6054 VBD denotes was
T18683 6063-6065 , denotes ,
T18684 6065-6070 VBN denotes sized
T18685 6071-6075 IN denotes onto
T18686 6076-6078 DT denotes an
T18687 6087-6090 NN denotes gel
T18688 6079-6086 NN denotes agarose
T18689 6090-6092 , denotes ,
T18690 6092-6095 CC denotes and
T18691 6096-6104 VBN denotes analyzed
T18692 6105-6107 IN denotes by
T18693 6108-6116 NNP denotes Southern
T18694 6117-6121 NN denotes blot
T18695 6121-6122 . denotes .
T18696 6122-6237 sentence denotes Hybridization signals were quantified using an Instant Imager (PerkinElmer, Wellesley, California, United States).
T18697 6123-6136 NN denotes Hybridization
T18698 6137-6144 NNS denotes signals
T18699 6150-6160 VBN denotes quantified
T18700 6145-6149 VBD denotes were
T18701 6161-6166 VBG denotes using
T18702 6167-6169 DT denotes an
T18703 6178-6184 NNP denotes Imager
T18704 6170-6177 NNP denotes Instant
T18705 6185-6186 -LRB- denotes (
T18706 6186-6197 NNP denotes PerkinElmer
T18707 6197-6199 , denotes ,
T18708 6199-6208 NNP denotes Wellesley
T18709 6208-6210 , denotes ,
T18710 6210-6220 NNP denotes California
T18711 6220-6222 , denotes ,
T18712 6222-6228 NNP denotes United
T18713 6229-6235 NNP denotes States
T18714 6235-6236 -RRB- denotes )
T18715 6236-6237 . denotes .
T18716 6237-6297 sentence denotes Antibodies specific for H4K20me3 were purchased from Abcam.
T18717 6238-6248 NNS denotes Antibodies
T18718 6276-6285 VBN denotes purchased
T18719 6249-6257 JJ denotes specific
T18720 6258-6261 IN denotes for
T18721 6262-6270 NN denotes H4K20me3
T18722 6271-6275 VBD denotes were
T18723 6286-6290 IN denotes from
T18724 6291-6296 NNP denotes Abcam
T18725 6296-6297 . denotes .
T18726 6297-6403 sentence denotes Antibodies for H3K9me3 were purchased from both Abcam and Upstate Biotechnology and gave similar results.
T18727 6298-6308 NNS denotes Antibodies
T18728 6326-6335 VBN denotes purchased
T18729 6309-6312 IN denotes for
T18730 6313-6320 NN denotes H3K9me3
T18731 6321-6325 VBD denotes were
T18732 6336-6340 IN denotes from
T18733 6341-6345 CC denotes both
T18734 6346-6351 NNP denotes Abcam
T18735 6352-6355 CC denotes and
T18736 6356-6363 NNP denotes Upstate
T18737 6364-6377 NNP denotes Biotechnology
T18738 6378-6381 CC denotes and
T18739 6382-6386 VBD denotes gave
T18740 6387-6394 JJ denotes similar
T18741 6395-6402 NNS denotes results
T18742 6402-6403 . denotes .
T18743 6403-6631 sentence denotes For Southern blot analysis, we used a 240-bp EcoRI/BamHI fragment from pSAT (major satellite probe) [45–47] and a 360-bp EcoRI/HindIII fragment from R198, corresponding to three copies of the 120-bp minor satellite repeat [49].
T18744 6404-6407 IN denotes For
T18745 6435-6439 VBD denotes used
T18746 6408-6416 NNP denotes Southern
T18747 6417-6421 NN denotes blot
T18748 6422-6430 NN denotes analysis
T18749 6430-6432 , denotes ,
T18750 6432-6434 PRP denotes we
T18751 6440-6441 DT denotes a
T18752 6461-6469 NN denotes fragment
T18753 6442-6445 CD denotes 240
T18754 6446-6448 NN denotes bp
T18755 6445-6446 HYPH denotes -
T18756 6449-6454 NN denotes EcoRI
T18757 6455-6460 NN denotes BamHI
T18758 6454-6455 HYPH denotes /
T18759 6470-6474 IN denotes from
T18760 6475-6479 NN denotes pSAT
T18761 6480-6481 -LRB- denotes (
T18762 6497-6502 NN denotes probe
T18763 6481-6486 JJ denotes major
T18764 6487-6496 NN denotes satellite
T18765 6502-6503 -RRB- denotes )
T18766 6504-6505 -LRB- denotes [
T18767 6505-6507 CD denotes 45
T18768 6507-6508 SYM denotes –
T18769 6508-6510 CD denotes 47
T18770 6510-6511 -RRB- denotes ]
T18771 6512-6515 CC denotes and
T18772 6516-6517 DT denotes a
T18773 6539-6547 NN denotes fragment
T18774 6518-6521 CD denotes 360
T18775 6522-6524 NN denotes bp
T18776 6521-6522 HYPH denotes -
T18777 6525-6530 NN denotes EcoRI
T18778 6531-6538 NN denotes HindIII
T18779 6530-6531 HYPH denotes /
T18780 6548-6552 IN denotes from
T18781 6553-6557 NN denotes R198
T18782 6557-6559 , denotes ,
T18783 6559-6572 VBG denotes corresponding
T18784 6573-6575 IN denotes to
T18785 6576-6581 CD denotes three
T18786 6582-6588 NNS denotes copies
T18787 6589-6591 IN denotes of
T18788 6592-6595 DT denotes the
T18789 6619-6625 NN denotes repeat
T18790 6596-6599 CD denotes 120
T18791 6600-6602 NN denotes bp
T18792 6599-6600 HYPH denotes -
T18793 6603-6608 JJ denotes minor
T18794 6609-6618 NN denotes satellite
T18795 6626-6627 -LRB- denotes [
T18796 6627-6629 CD denotes 49
T18797 6629-6630 -RRB- denotes ]
T18798 6630-6631 . denotes .
T18799 6631-6690 sentence denotes Probes used in FISH experiments were described in [46–48].
T18800 6632-6638 NNS denotes Probes
T18801 6669-6678 VBN denotes described
T18802 6639-6643 VBN denotes used
T18803 6644-6646 IN denotes in
T18804 6647-6651 NN denotes FISH
T18805 6652-6663 NNS denotes experiments
T18806 6664-6668 VBD denotes were
T18807 6679-6681 IN denotes in
T18808 6682-6683 -LRB- denotes [
T18809 6683-6685 CD denotes 46
T18810 6685-6686 SYM denotes –
T18811 6686-6688 CD denotes 48
T18812 6688-6689 -RRB- denotes ]
T18813 6689-6690 . denotes .
T18814 6690-6740 sentence denotes The probe for IAP elements was described in [50].
T18815 6691-6694 DT denotes The
T18816 6695-6700 NN denotes probe
T18817 6722-6731 VBN denotes described
T18818 6701-6704 IN denotes for
T18819 6705-6708 NN denotes IAP
T18820 6709-6717 NNS denotes elements
T18821 6718-6721 VBD denotes was
T18822 6732-6734 IN denotes in
T18823 6735-6736 -LRB- denotes [
T18824 6736-6738 CD denotes 50
T18825 6738-6739 -RRB- denotes ]
T18826 6739-6740 . denotes .
R4195 T14829 T14828 prep of,Generation
R4196 T14830 T14831 compound Chaf1a,mutant
R4197 T14831 T14829 pobj mutant,of
R4198 T14832 T14831 appos mice,mutant
R4199 T14833 T14832 cc and,mice
R4200 T14834 T14832 conj embryos,mice
R4201 T14835 T14828 punct .,Generation
R4202 T14837 T14838 advcl Using,amplified
R4203 T14839 T14837 dobj PCR,Using
R4204 T14840 T14838 punct ", ",amplified
R4205 T14841 T14838 nsubj we,amplified
R4206 T14842 T14843 nummod two,fragments
R4207 T14843 T14838 dobj fragments,amplified
R4208 T14844 T14843 amod genomic,fragments
R4209 T14845 T14846 punct (,kb
R4210 T14846 T14843 parataxis kb,fragments
R4211 T14847 T14848 quantmod about,3
R4212 T14848 T14846 nummod 3,kb
R4213 T14849 T14846 appos each,kb
R4214 T14850 T14846 punct ),kb
R4215 T14851 T14843 acl flanking,fragments
R4216 T14852 T14853 compound Chaf1a,exon
R4217 T14853 T14851 dobj exon,flanking
R4218 T14854 T14853 nummod 3,exon
R4219 T14855 T14838 punct .,amplified
R4220 T14857 T14858 det These,fragments
R4221 T14858 T14860 nsubjpass fragments,assembled
R4222 T14859 T14858 compound DNA,fragments
R4223 T14861 T14860 auxpass were,assembled
R4224 T14862 T14860 prep by,assembled
R4225 T14863 T14864 amod conventional,cloning
R4226 T14864 T14862 pobj cloning,by
R4227 T14865 T14864 prep with,cloning
R4228 T14866 T14867 det the,cassettes
R4229 T14867 T14865 pobj cassettes,with
R4230 T14868 T14867 nmod neomycin,cassettes
R4231 T14869 T14868 cc and,neomycin
R4232 T14870 T14868 conj DT,neomycin
R4233 T14871 T14870 punct (,DT
R4234 T14872 T14873 compound diphtheria,toxin
R4235 T14873 T14870 appos toxin,DT
R4236 T14874 T14867 punct ),cassettes
R4237 T14875 T14860 punct ", ",assembled
R4238 T14876 T14877 mark as,described
R4239 T14877 T14860 advcl described,assembled
R4240 T14878 T14877 prep in,described
R4241 T14879 T14880 compound Figure,1A
R4242 T14880 T14878 pobj 1A,in
R4243 T14881 T14860 punct .,assembled
R4244 T14883 T14884 det The,construct
R4245 T14884 T14885 nsubjpass construct,transfected
R4246 T14886 T14885 auxpass was,transfected
R4247 T14887 T14885 prep in,transfected
R4248 T14888 T14889 compound ES,cells
R4249 T14889 T14887 pobj cells,in
R4250 T14890 T14885 prep by,transfected
R4251 T14891 T14890 pobj electroporation,by
R4252 T14892 T14885 punct .,transfected
R4253 T14894 T14895 nsubj We,identified
R4254 T14896 T14897 amod recombinant,cells
R4255 T14897 T14895 dobj cells,identified
R4256 T14898 T14897 compound ES,cells
R4257 T14899 T14897 acl carrying,cells
R4258 T14900 T14901 det the,allele
R4259 T14901 T14899 dobj allele,carrying
R4260 T14902 T14901 nmod mutant,allele
R4261 T14903 T14901 nmod Chaf1a tm1Ger,allele
R4262 T14904 T14905 punct (,abbreviated
R4263 T14905 T14903 parataxis abbreviated,Chaf1a tm1Ger
R4264 T14906 T14905 oprd Chaf1a,abbreviated
R4265 T14907 T14906 punct −,Chaf1a
R4266 T14908 T14905 prep in,abbreviated
R4267 T14909 T14910 det the,manuscript
R4268 T14910 T14908 pobj manuscript,in
R4269 T14911 T14905 punct ),abbreviated
R4270 T14912 T14895 prep by,identified
R4271 T14913 T14914 compound Southern,blot
R4272 T14914 T14912 pobj blot,by
R4273 T14915 T14916 punct (,1A
R4274 T14916 T14895 parataxis 1A,identified
R4275 T14917 T14916 compound Figure,1A
R4276 T14918 T14916 punct ),1A
R4277 T14919 T14895 punct .,identified
R4278 T14921 T14922 nsubj We,derived
R4279 T14923 T14924 nmod Chaf1a,mice
R4280 T14924 T14922 dobj mice,derived
R4281 T14925 T14923 punct +,Chaf1a
R4282 T14926 T14923 punct /,Chaf1a
R4283 T14927 T14923 punct −,Chaf1a
R4284 T14928 T14922 prep by,derived
R4285 T14929 T14928 pcomp injecting,by
R4286 T14930 T14931 amod recombined,cells
R4287 T14931 T14929 dobj cells,injecting
R4288 T14932 T14931 compound ES,cells
R4289 T14933 T14929 prep into,injecting
R4290 T14934 T14935 compound C57BL,6N
R4291 T14935 T14937 compound 6N,blastocysts
R4292 T14936 T14935 punct /,6N
R4293 T14937 T14933 pobj blastocysts,into
R4294 T14938 T14922 punct .,derived
R4295 T14940 T14941 compound E4,embryos
R4296 T14941 T14942 nsubjpass embryos,genotyped
R4297 T14943 T14941 acl obtained,embryos
R4298 T14944 T14943 prep from,obtained
R4299 T14945 T14946 det the,intercross
R4300 T14946 T14944 pobj intercross,from
R4301 T14947 T14946 prep of,intercross
R4302 T14948 T14949 nmod Chaf1a,mice
R4303 T14949 T14947 pobj mice,of
R4304 T14950 T14948 punct +,Chaf1a
R4305 T14951 T14948 punct /,Chaf1a
R4306 T14952 T14948 punct −,Chaf1a
R4307 T14953 T14942 auxpass were,genotyped
R4308 T14954 T14942 prep by,genotyped
R4309 T14955 T14956 compound nested,PCR
R4310 T14956 T14958 compound PCR,amplification
R4311 T14957 T14956 punct -,PCR
R4312 T14958 T14954 pobj amplification,by
R4313 T14959 T14942 punct .,genotyped
R4314 T14961 T14962 nsubjpass Embryos,collected
R4315 T14963 T14962 auxpass were,collected
R4316 T14964 T14962 prep in,collected
R4317 T14965 T14966 det a,mix
R4318 T14966 T14964 pobj mix,in
R4319 T14967 T14966 compound PCR,mix
R4320 T14968 T14966 compound reaction,mix
R4321 T14969 T14966 acl containing,mix
R4322 T14970 T14971 nmod oligonucleotide,1
R4323 T14971 T14969 dobj 1,containing
R4324 T14972 T14971 nmod primers,1
R4325 T14973 T14971 punct ", ",1
R4326 T14974 T14971 conj 2,1
R4327 T14975 T14974 punct ", ",2
R4328 T14976 T14974 cc and,2
R4329 T14977 T14974 conj 3,2
R4330 T14978 T14979 punct (,1A
R4331 T14979 T14971 parataxis 1A,1
R4332 T14980 T14979 compound Figure,1A
R4333 T14981 T14979 punct ),1A
R4334 T14982 T14983 dep that,amplify
R4335 T14983 T14971 relcl amplify,1
R4336 T14984 T14985 det the,alleles
R4337 T14985 T14983 dobj alleles,amplify
R4338 T14986 T14987 amod wild,type
R4339 T14987 T14985 nmod type,alleles
R4340 T14988 T14987 punct -,type
R4341 T14989 T14987 cc and,type
R4342 T14990 T14987 conj mutant,type
R4343 T14991 T14962 punct .,collected
R4344 T14993 T14994 prep After,used
R4345 T14995 T14996 nummod 30,cycles
R4346 T14996 T14993 pobj cycles,After
R4347 T14997 T14996 prep of,cycles
R4348 T14998 T14997 pobj amplification,of
R4349 T14999 T14994 punct ", ",used
R4350 T15000 T15001 nummod 1,μl
R4351 T15001 T14994 nsubjpass μl,used
R4352 T15002 T15001 prep of,μl
R4353 T15003 T15004 det each,reaction
R4354 T15004 T15002 pobj reaction,of
R4355 T15005 T15004 compound PCR,reaction
R4356 T15006 T14994 auxpass was,used
R4357 T15007 T14994 prep in,used
R4358 T15008 T15009 det a,round
R4359 T15009 T15007 pobj round,in
R4360 T15010 T15009 amod second,round
R4361 T15011 T15009 prep of,round
R4362 T15012 T15013 compound PCR,amplification
R4363 T15013 T15011 pobj amplification,of
R4364 T15014 T15009 prep with,round
R4365 T15015 T15016 det a,set
R4366 T15016 T15014 pobj set,with
R4367 T15017 T15016 amod new,set
R4368 T15018 T15016 prep of,set
R4369 T15019 T15020 compound oligonucleotide,primers
R4370 T15020 T15018 pobj primers,of
R4371 T15021 T14994 punct .,used
R4372 T15023 T15024 prep After,run
R4373 T15025 T15026 nummod 30,cycles
R4374 T15026 T15023 pobj cycles,After
R4375 T15027 T15026 prep of,cycles
R4376 T15028 T15027 pobj amplification,of
R4377 T15029 T15024 punct ", ",run
R4378 T15030 T15031 compound PCR,reactions
R4379 T15031 T15024 nsubjpass reactions,run
R4380 T15032 T15024 auxpass were,run
R4381 T15033 T15024 prep onto,run
R4382 T15034 T15035 det an,gel
R4383 T15035 T15033 pobj gel,onto
R4384 T15036 T15035 compound agarose,gel
R4385 T15037 T15035 punct ", ",gel
R4386 T15038 T15039 dep which,revealed
R4387 T15039 T15035 relcl revealed,gel
R4388 T15040 T15041 det the,presence
R4389 T15041 T15039 dobj presence,revealed
R4390 T15042 T15041 prep of,presence
R4391 T15043 T15044 det the,alleles
R4392 T15044 T15042 pobj alleles,of
R4393 T15045 T15046 amod wild,type
R4394 T15046 T15044 nmod type,alleles
R4395 T15047 T15046 punct -,type
R4396 T15048 T15049 punct (,bp
R4397 T15049 T15046 parataxis bp,type
R4398 T15050 T15049 nummod 150,bp
R4399 T15051 T15049 punct ),bp
R4400 T15052 T15046 cc and,type
R4401 T15053 T15046 conj recombinant,type
R4402 T15054 T15055 punct (,bp
R4403 T15055 T15053 parataxis bp,recombinant
R4404 T15056 T15055 nummod 200,bp
R4405 T15057 T15055 punct ),bp
R4406 T15058 T15024 punct .,run
R4407 T15693 T15694 nmod P150CAF,depletion
R4408 T15695 T15693 punct -,P150CAF
R4409 T15696 T15693 nummod 1,P150CAF
R4410 T15697 T15694 prep by,depletion
R4411 T15698 T15697 pobj RNAi,by
R4412 T15699 T15694 punct .,depletion
R4413 T15701 T15702 det The,vector
R4414 T15702 T15705 nsubj vector,contains
R4415 T15703 T15702 compound RNAi,vector
R4416 T15704 T15702 compound plasmid,vector
R4417 T15706 T15707 dep that,used
R4418 T15707 T15702 relcl used,vector
R4419 T15708 T15707 nsubj we,used
R4420 T15709 T15707 prep in,used
R4421 T15710 T15711 det this,study
R4422 T15711 T15709 pobj study,in
R4423 T15712 T15713 det the,promoter
R4424 T15713 T15705 dobj promoter,contains
R4425 T15714 T15713 compound mouse,promoter
R4426 T15715 T15713 compound H1,promoter
R4427 T15716 T15713 cc and,promoter
R4428 T15717 T15718 det a,gene
R4429 T15718 T15713 conj gene,promoter
R4430 T15719 T15718 compound puromycin,gene
R4431 T15720 T15718 compound selection,gene
R4432 T15721 T15722 punct (,data
R4433 T15722 T15705 parataxis data,contains
R4434 T15723 T15722 dep SB,data
R4435 T15724 T15723 cc and,SB
R4436 T15725 T15723 conj MG,SB
R4437 T15726 T15722 punct ", ",data
R4438 T15727 T15722 amod unpublished,data
R4439 T15728 T15722 punct ),data
R4440 T15729 T15705 punct .,contains
R4441 T15731 T15732 nsubj We,characterized
R4442 T15732 T15733 ccomp characterized,is
R4443 T15734 T15732 prep in,characterized
R4444 T15735 T15734 pobj detail,in
R4445 T15736 T15737 det the,properties
R4446 T15737 T15732 dobj properties,characterized
R4447 T15738 T15737 prep of,properties
R4448 T15739 T15740 det this,vector
R4449 T15740 T15738 pobj vector,of
R4450 T15741 T15740 amod new,vector
R4451 T15742 T15732 advcl using,characterized
R4452 T15743 T15744 det a,gene
R4453 T15744 T15742 dobj gene,using
R4454 T15745 T15744 compound GFP,gene
R4455 T15746 T15744 compound target,gene
R4456 T15747 T15744 cc and,gene
R4457 T15748 T15744 conj FACS,gene
R4458 T15749 T15733 punct : ,is
R4459 T15750 T15733 nsubj Extinction,is
R4460 T15751 T15750 prep of,Extinction
R4461 T15752 T15753 compound target,genes
R4462 T15753 T15751 pobj genes,of
R4463 T15754 T15750 prep in,Extinction
R4464 T15755 T15756 compound ES,cells
R4465 T15756 T15754 pobj cells,in
R4466 T15757 T15758 advmod highly,efficient
R4467 T15758 T15733 acomp efficient,is
R4468 T15759 T15733 prep in,is
R4469 T15760 T15761 nummod 70,%
R4470 T15761 T15759 pobj %,in
R4471 T15762 T15761 prep of,%
R4472 T15763 T15764 det the,cells
R4473 T15764 T15762 pobj cells,of
R4474 T15765 T15733 punct ", ",is
R4475 T15766 T15733 conj of,is
R4476 T15767 T15768 amod intermediate,efficiency
R4477 T15768 T15766 pobj efficiency,of
R4478 T15769 T15766 prep in,of
R4479 T15770 T15771 nummod 15,%
R4480 T15771 T15769 pobj %,in
R4481 T15772 T15766 punct ", ",of
R4482 T15773 T15766 cc and,of
R4483 T15774 T15766 conj inefficient,of
R4484 T15775 T15774 prep in,inefficient
R4485 T15776 T15777 det the,%
R4486 T15777 T15775 pobj %,in
R4487 T15778 T15777 amod remaining,%
R4488 T15779 T15777 nummod 15,%
R4489 T15780 T15777 prep of,%
R4490 T15781 T15782 compound cells,analysis
R4491 T15782 T15780 pobj analysis,of
R4492 T15783 T15733 punct .,is
R4493 T15785 T15786 det The,sequence
R4494 T15786 T15787 nsubjpass sequence,cloned
R4495 T15788 T15786 prep of,sequence
R4496 T15789 T15790 nmod p150CAF,duplex
R4497 T15790 T15788 pobj duplex,of
R4498 T15791 T15789 punct -,p150CAF
R4499 T15792 T15789 nummod 1,p150CAF
R4500 T15793 T15790 nmod siRNA,duplex
R4501 T15794 T15793 punct (,siRNA
R4502 T15795 T15796 amod short,RNA
R4503 T15796 T15793 appos RNA,siRNA
R4504 T15797 T15796 amod interfering,RNA
R4505 T15798 T15790 punct ),duplex
R4506 T15799 T15800 punct [,16
R4507 T15800 T15786 parataxis 16,sequence
R4508 T15801 T15800 punct ],16
R4509 T15802 T15787 auxpass was,cloned
R4510 T15803 T15787 prep into,cloned
R4511 T15804 T15805 det this,plasmid
R4512 T15805 T15803 pobj plasmid,into
R4513 T15806 T15787 punct .,cloned
R4514 T15808 T15809 det The,vector
R4515 T15809 T15811 nsubj vector,expressed
R4516 T15810 T15809 compound control,vector
R4517 T15812 T15813 det a,siRNA
R4518 T15813 T15811 dobj siRNA,expressed
R4519 T15814 T15815 dep that,targets
R4520 T15815 T15813 relcl targets,siRNA
R4521 T15816 T15817 compound GFP,degradation
R4522 T15817 T15815 dobj degradation,targets
R4523 T15818 T15817 compound RNA,degradation
R4524 T15819 T15811 punct .,expressed
R4525 T15821 T15822 compound ES,cells
R4526 T15822 T15823 nsubjpass cells,transfected
R4527 T15824 T15823 auxpass were,transfected
R4528 T15825 T15823 prep by,transfected
R4529 T15826 T15825 pobj electroporation,by
R4530 T15827 T15823 prep with,transfected
R4531 T15828 T15829 det the,vector
R4532 T15829 T15827 pobj vector,with
R4533 T15830 T15829 compound RNA,vector
R4534 T15831 T15829 compound plasmid,vector
R4535 T15832 T15823 punct ", ",transfected
R4536 T15833 T15823 conj seeded,transfected
R4537 T15834 T15833 prep onto,seeded
R4538 T15835 T15836 npadvmod gelatin,coated
R4539 T15836 T15838 amod coated,slides
R4540 T15837 T15836 punct -,coated
R4541 T15838 T15834 pobj slides,onto
R4542 T15839 T15833 punct ", ",seeded
R4543 T15840 T15833 cc and,seeded
R4544 T15841 T15833 conj cultured,seeded
R4545 T15842 T15841 prep for,cultured
R4546 T15843 T15844 nummod 24,h
R4547 T15844 T15842 pobj h,for
R4548 T15845 T15841 prep in,cultured
R4549 T15846 T15847 det the,absence
R4550 T15847 T15845 pobj absence,in
R4551 T15848 T15847 prep of,absence
R4552 T15849 T15848 pobj selection,of
R4553 T15850 T15823 punct .,transfected
R4554 T15852 T15853 nsubjpass Puromycin,added
R4555 T15854 T15855 punct (,μg
R4556 T15855 T15852 parataxis μg,Puromycin
R4557 T15856 T15855 nummod 2,μg
R4558 T15857 T15858 punct /,ml
R4559 T15858 T15855 prep ml,μg
R4560 T15859 T15855 punct ),μg
R4561 T15860 T15853 auxpass was,added
R4562 T15861 T15853 prep to,added
R4563 T15862 T15863 det the,medium
R4564 T15863 T15861 pobj medium,to
R4565 T15864 T15863 compound culture,medium
R4566 T15865 T15853 cc and,added
R4567 T15866 T15867 nsubjpass cells,cultured
R4568 T15867 T15853 conj cultured,added
R4569 T15868 T15867 auxpass were,cultured
R4570 T15869 T15867 prep for,cultured
R4571 T15870 T15871 det an,period
R4572 T15871 T15869 pobj period,for
R4573 T15872 T15871 amod additional,period
R4574 T15873 T15874 nummod 48,h
R4575 T15874 T15871 compound h,period
R4576 T15875 T15874 punct -,h
R4577 T15876 T15867 punct .,cultured
R4578 T15878 T15879 advcl Using,reveals
R4579 T15880 T15881 det the,vector
R4580 T15881 T15878 dobj vector,Using
R4581 T15882 T15881 nmod p150CAF,vector
R4582 T15883 T15882 punct -,p150CAF
R4583 T15884 T15882 nummod 1,p150CAF
R4584 T15885 T15881 compound RNAi,vector
R4585 T15886 T15879 punct ", ",reveals
R4586 T15887 T15888 compound IF,microscopy
R4587 T15888 T15889 compound microscopy,quantification
R4588 T15889 T15879 nsubj quantification,reveals
R4589 T15890 T15891 det a,depletion
R4590 T15891 T15879 dobj depletion,reveals
R4591 T15892 T15891 amod complete,depletion
R4592 T15893 T15891 nmod CAF,depletion
R4593 T15894 T15893 punct -,CAF
R4594 T15895 T15893 nummod 1,CAF
R4595 T15896 T15879 prep in,reveals
R4596 T15897 T15898 amod most,cells
R4597 T15898 T15896 pobj cells,in
R4598 T15899 T15900 punct (,S1B
R4599 T15900 T15879 parataxis S1B,reveals
R4600 T15901 T15900 compound Figure,S1B
R4601 T15902 T15900 punct ),S1B
R4602 T15903 T15879 punct .,reveals
R4603 T15905 T15906 compound Western,blot
R4604 T15906 T15907 compound blot,analysis
R4605 T15907 T15908 nsubj analysis,reveals
R4606 T15909 T15910 punct (,S1A
R4607 T15910 T15907 parataxis S1A,analysis
R4608 T15911 T15910 compound Figure,S1A
R4609 T15912 T15910 punct ),S1A
R4610 T15913 T15914 amod residual,expression
R4611 T15914 T15908 dobj expression,reveals
R4612 T15915 T15914 nmod p150CAF,expression
R4613 T15916 T15915 punct -,p150CAF
R4614 T15917 T15915 nummod 1,p150CAF
R4615 T15918 T15908 punct ", ",reveals
R4616 T15919 T15920 mark as,expected
R4617 T15920 T15908 advcl expected,reveals
R4618 T15921 T15920 prep from,expected
R4619 T15922 T15923 det the,%
R4620 T15923 T15921 pobj %,from
R4621 T15924 T15923 nummod 15,%
R4622 T15925 T15923 prep of,%
R4623 T15926 T15925 pobj cells,of
R4624 T15927 T15928 dep that,inhibit
R4625 T15928 T15923 relcl inhibit,%
R4626 T15929 T15928 aux do,inhibit
R4627 T15930 T15928 neg not,inhibit
R4628 T15931 T15932 det the,gene
R4629 T15932 T15928 dobj gene,inhibit
R4630 T15933 T15932 compound target,gene
R4631 T15934 T15908 punct .,reveals
R4632 T15936 T15937 nsubjpass MEFs,transfected
R4633 T15938 T15939 punct (,passage
R4634 T15939 T15936 parataxis passage,MEFs
R4635 T15940 T15939 nummod 3,passage
R4636 T15941 T15939 punct ", ",passage
R4637 T15942 T15943 nummod 90,%
R4638 T15943 T15944 compound %,confluence
R4639 T15944 T15939 appos confluence,passage
R4640 T15945 T15939 punct ),passage
R4641 T15946 T15937 auxpass were,transfected
R4642 T15947 T15937 prep during,transfected
R4643 T15948 T15949 nummod 4,h
R4644 T15949 T15947 pobj h,during
R4645 T15950 T15949 prep with,h
R4646 T15951 T15952 det the,vector
R4647 T15952 T15950 pobj vector,with
R4648 T15953 T15952 compound RNAi,vector
R4649 T15954 T15952 compound plasmid,vector
R4650 T15955 T15937 advcl using,transfected
R4651 T15956 T15955 dobj Lipofectamine,using
R4652 T15957 T15956 nummod 2000,Lipofectamine
R4653 T15958 T15959 punct (,Invitrogene
R4654 T15959 T15956 parataxis Invitrogene,Lipofectamine
R4655 T15960 T15959 punct ", ",Invitrogene
R4656 T15961 T15959 npadvmod Carlsbad,Invitrogene
R4657 T15962 T15959 punct ", ",Invitrogene
R4658 T15963 T15959 npadvmod California,Invitrogene
R4659 T15964 T15959 punct ", ",Invitrogene
R4660 T15965 T15966 compound United,States
R4661 T15966 T15959 npadvmod States,Invitrogene
R4662 T15967 T15959 punct ),Invitrogene
R4663 T15968 T15937 prep according,transfected
R4664 T15969 T15968 prep to,according
R4665 T15970 T15971 poss manufacturer,conditions
R4666 T15971 T15969 pobj conditions,to
R4667 T15972 T15970 case 's,manufacturer
R4668 T15973 T15937 punct .,transfected
R4669 T15975 T15976 nsubjpass Cells,trypsinized
R4670 T15977 T15976 auxpass were,trypsinized
R4671 T15978 T15976 punct ", ",trypsinized
R4672 T15979 T15976 conj plated,trypsinized
R4673 T15980 T15979 prep at,plated
R4674 T15981 T15982 quantmod 1,3
R4675 T15982 T15984 nummod 3,dilution
R4676 T15983 T15982 punct /,3
R4677 T15984 T15980 pobj dilution,at
R4678 T15985 T15979 punct ", ",plated
R4679 T15986 T15979 cc and,plated
R4680 T15987 T15979 conj cultured,plated
R4681 T15988 T15987 prep for,cultured
R4682 T15989 T15990 nummod 48,h
R4683 T15990 T15988 pobj h,for
R4684 T15991 T15987 prep in,cultured
R4685 T15992 T15993 det the,presence
R4686 T15993 T15991 pobj presence,in
R4687 T15994 T15993 prep of,presence
R4688 T15995 T15994 pobj puromycin,of
R4689 T15996 T15976 punct .,trypsinized
R4690 T16173 T16174 compound Cell,lines
R4691 T16175 T16174 punct .,lines
R4692 T16177 T16178 nmod AT,cells
R4693 T16178 T16182 nsubjpass cells,used
R4694 T16179 T16177 punct -,AT
R4695 T16180 T16177 nummod 1,AT
R4696 T16181 T16178 compound ES,cells
R4697 T16183 T16184 punct [,42
R4698 T16184 T16178 parataxis 42,cells
R4699 T16185 T16184 punct ],42
R4700 T16186 T16187 punct (,gift
R4701 T16187 T16178 parataxis gift,cells
R4702 T16188 T16187 det a,gift
R4703 T16189 T16187 prep of,gift
R4704 T16190 T16191 compound M.,Vernet
R4705 T16191 T16189 pobj Vernet,of
R4706 T16192 T16187 punct ),gift
R4707 T16193 T16182 auxpass were,used
R4708 T16194 T16182 prep for,used
R4709 T16195 T16196 compound gene,targeting
R4710 T16196 T16194 pobj targeting,for
R4711 T16197 T16182 punct .,used
R4712 T16199 T16200 compound LTM7,cells
R4713 T16200 T16202 nsubjpass cells,used
R4714 T16201 T16200 compound ES,cells
R4715 T16203 T16202 auxpass were,used
R4716 T16204 T16202 prep in,used
R4717 T16205 T16206 det all,experiments
R4718 T16206 T16204 pobj experiments,in
R4719 T16207 T16206 compound RNAi,experiments
R4720 T16208 T16202 punct .,used
R4721 T16210 T16211 nsubj We,derived
R4722 T16212 T16213 det this,line
R4723 T16213 T16211 dobj line,derived
R4724 T16214 T16213 compound cell,line
R4725 T16215 T16211 prep from,derived
R4726 T16216 T16217 punct (,C57BL
R4727 T16217 T16218 nmod C57BL,females
R4728 T16218 T16215 pobj females,from
R4729 T16219 T16217 punct /,C57BL
R4730 T16220 T16217 nummod 6,C57BL
R4731 T16221 T16217 punct ×,C57BL
R4732 T16222 T16217 appos 129,C57BL
R4733 T16223 T16217 punct ),C57BL
R4734 T16224 T16218 compound F1,females
R4735 T16225 T16218 acl bred,females
R4736 T16226 T16225 prep with,bred
R4737 T16227 T16228 compound C3H,HeJ
R4738 T16228 T16230 compound HeJ,males
R4739 T16229 T16228 punct /,HeJ
R4740 T16230 T16226 pobj males,with
R4741 T16231 T16211 punct .,derived
R4742 T16233 T16234 nsubj LTM7,are
R4743 T16235 T16236 compound XX,cells
R4744 T16236 T16234 attr cells,are
R4745 T16237 T16236 compound ES,cells
R4746 T16238 T16236 punct ", ",cells
R4747 T16239 T16236 amod competent,cells
R4748 T16240 T16239 prep for,competent
R4749 T16241 T16242 compound germ,line
R4750 T16242 T16243 compound line,transmission
R4751 T16243 T16240 pobj transmission,for
R4752 T16244 T16234 punct .,are
R4753 T16246 T16247 amod Primary,MEFs
R4754 T16247 T16248 nsubjpass MEFs,derived
R4755 T16249 T16248 auxpass were,derived
R4756 T16250 T16248 prep from,derived
R4757 T16251 T16252 compound E13,embryos
R4758 T16252 T16250 pobj embryos,from
R4759 T16253 T16254 mark as,described
R4760 T16254 T16248 advcl described,derived
R4761 T16255 T16254 prep in,described
R4762 T16256 T16255 punct [,in
R4763 T16257 T16255 pobj 43,in
R4764 T16258 T16248 punct ],derived
R4765 T16259 T16248 punct .,derived
R4766 T16588 T16587 punct .,Immunofluorescence
R4767 T16590 T16591 nsubjpass Cells,fixed
R4768 T16592 T16591 auxpass were,fixed
R4769 T16593 T16591 prep for,fixed
R4770 T16594 T16595 nummod 20,min
R4771 T16595 T16593 pobj min,for
R4772 T16596 T16591 prep in,fixed
R4773 T16597 T16596 pobj PBS,in
R4774 T16598 T16591 prep with,fixed
R4775 T16599 T16600 nummod 4,%
R4776 T16600 T16601 compound %,paraformaldehyde
R4777 T16601 T16598 pobj paraformaldehyde,with
R4778 T16602 T16591 punct ", ",fixed
R4779 T16603 T16591 cc and,fixed
R4780 T16604 T16605 nsubjpass immunodetection,performed
R4781 T16605 T16591 conj performed,fixed
R4782 T16606 T16605 auxpass was,performed
R4783 T16607 T16608 mark as,described
R4784 T16608 T16605 advcl described,performed
R4785 T16609 T16608 advmod previously,described
R4786 T16610 T16611 punct [,44
R4787 T16611 T16605 parataxis 44,performed
R4788 T16612 T16611 punct ],44
R4789 T16613 T16605 punct .,performed
R4790 T16615 T16616 compound E4,embryos
R4791 T16616 T16617 nsubjpass embryos,collected
R4792 T16618 T16617 auxpass were,collected
R4793 T16619 T16617 cc and,collected
R4794 T16620 T16617 conj treated,collected
R4795 T16621 T16620 prep with,treated
R4796 T16622 T16623 compound tyrode,acid
R4797 T16623 T16621 pobj acid,with
R4798 T16624 T16625 aux to,remove
R4799 T16625 T16620 advcl remove,treated
R4800 T16626 T16627 det the,pellucida
R4801 T16627 T16625 dobj pellucida,remove
R4802 T16628 T16627 compound zona,pellucida
R4803 T16629 T16620 punct ", ",treated
R4804 T16630 T16620 conj deposited,treated
R4805 T16631 T16630 prep onto,deposited
R4806 T16632 T16633 compound microscope,slides
R4807 T16633 T16631 pobj slides,onto
R4808 T16634 T16630 punct ", ",deposited
R4809 T16635 T16630 cc and,deposited
R4810 T16636 T16630 conj processed,deposited
R4811 T16637 T16636 prep for,processed
R4812 T16638 T16637 pobj immunostaining,for
R4813 T16639 T16640 mark as,described
R4814 T16640 T16636 advcl described,processed
R4815 T16641 T16640 prep for,described
R4816 T16642 T16643 compound ES,cells
R4817 T16643 T16641 pobj cells,for
R4818 T16644 T16617 punct .,collected
R4819 T16646 T16647 nsubjpass Antibodies,used
R4820 T16648 T16646 appos anti-HP1α,Antibodies
R4821 T16649 T16650 punct (,2HP1H5
R4822 T16650 T16648 parataxis 2HP1H5,anti-HP1α
R4823 T16651 T16650 punct ", ",2HP1H5
R4824 T16652 T16650 npadvmod Euromedex,2HP1H5
R4825 T16653 T16650 punct ", ",2HP1H5
R4826 T16654 T16650 npadvmod France,2HP1H5
R4827 T16655 T16650 punct ),2HP1H5
R4828 T16656 T16648 punct ", ",anti-HP1α
R4829 T16657 T16648 conj anti-H4K20me3,anti-HP1α
R4830 T16658 T16659 punct (,Abcam
R4831 T16659 T16657 parataxis Abcam,anti-H4K20me3
R4832 T16660 T16659 punct ", ",Abcam
R4833 T16661 T16659 npadvmod Cambridge,Abcam
R4834 T16662 T16659 punct ", ",Abcam
R4835 T16663 T16664 compound United,Kingdom
R4836 T16664 T16659 npadvmod Kingdom,Abcam
R4837 T16665 T16659 punct ),Abcam
R4838 T16666 T16657 punct ", ",anti-H4K20me3
R4839 T16667 T16657 conj anti-BrdU,anti-H4K20me3
R4840 T16668 T16669 punct (,DakoCytomation
R4841 T16669 T16667 parataxis DakoCytomation,anti-BrdU
R4842 T16670 T16669 punct ", ",DakoCytomation
R4843 T16671 T16669 npadvmod Glostrup,DakoCytomation
R4844 T16672 T16669 punct ", ",DakoCytomation
R4845 T16673 T16669 npadvmod Denmark,DakoCytomation
R4846 T16674 T16669 punct ),DakoCytomation
R4847 T16675 T16667 punct ", ",anti-BrdU
R4848 T16676 T16667 conj anti-PCNA,anti-BrdU
R4849 T16677 T16678 punct (,DakoCytomation
R4850 T16678 T16676 parataxis DakoCytomation,anti-PCNA
R4851 T16679 T16678 punct ),DakoCytomation
R4852 T16680 T16676 punct ", ",anti-PCNA
R4853 T16681 T16676 cc and,anti-PCNA
R4854 T16682 T16676 conj anti-PML,anti-PCNA
R4855 T16683 T16684 punct (,Biotechnology
R4856 T16684 T16682 parataxis Biotechnology,anti-PML
R4857 T16685 T16684 compound Upstate,Biotechnology
R4858 T16686 T16684 punct ", ",Biotechnology
R4859 T16687 T16688 compound Lake,Placid
R4860 T16688 T16684 npadvmod Placid,Biotechnology
R4861 T16689 T16684 punct ", ",Biotechnology
R4862 T16690 T16691 compound New,York
R4863 T16691 T16684 npadvmod York,Biotechnology
R4864 T16692 T16684 punct ", ",Biotechnology
R4865 T16693 T16694 compound United,States
R4866 T16694 T16684 npadvmod States,Biotechnology
R4867 T16695 T16684 punct ),Biotechnology
R4868 T16696 T16647 auxpass were,used
R4869 T16697 T16647 advmod all,used
R4870 T16698 T16647 prep at,used
R4871 T16699 T16700 nummod 1,dilution
R4872 T16700 T16698 pobj dilution,at
R4873 T16701 T16702 punct /,1000
R4874 T16702 T16699 prep 1000,1
R4875 T16703 T16647 punct .,used
R4876 T16705 T16706 nmod Antibodies,p150CAF1
R4877 T16706 T16708 nsubjpass p150CAF1,used
R4878 T16707 T16706 amod anti-mouse,p150CAF1
R4879 T16709 T16710 punct [,16
R4880 T16710 T16706 parataxis 16,p150CAF1
R4881 T16711 T16710 punct ],16
R4882 T16712 T16706 cc and,p150CAF1
R4883 T16713 T16706 conj anti-H3K9me3,p150CAF1
R4884 T16714 T16715 punct (,Biotechnology
R4885 T16715 T16713 parataxis Biotechnology,anti-H3K9me3
R4886 T16716 T16715 compound Upstate,Biotechnology
R4887 T16717 T16715 punct ),Biotechnology
R4888 T16718 T16708 auxpass were,used
R4889 T16719 T16708 prep at,used
R4890 T16720 T16719 pobj 1,at
R4891 T16721 T16722 punct /,250
R4892 T16722 T16720 prep 250,1
R4893 T16723 T16720 cc and,1
R4894 T16724 T16720 conj 1,1
R4895 T16725 T16726 punct /,500
R4896 T16726 T16724 prep 500,1
R4897 T16727 T16708 punct ", ",used
R4898 T16728 T16708 advmod respectively,used
R4899 T16729 T16708 punct .,used
R4900 T16731 T16732 det All,antibodies
R4901 T16732 T16734 nsubjpass antibodies,purchased
R4902 T16733 T16732 amod secondary,antibodies
R4903 T16735 T16734 auxpass were,purchased
R4904 T16736 T16734 prep from,purchased
R4905 T16737 T16738 compound Molecular,Probes
R4906 T16738 T16736 pobj Probes,from
R4907 T16739 T16740 punct (,Sunnyvale
R4908 T16740 T16738 parataxis Sunnyvale,Probes
R4909 T16741 T16740 punct ", ",Sunnyvale
R4910 T16742 T16740 npadvmod California,Sunnyvale
R4911 T16743 T16740 punct ", ",Sunnyvale
R4912 T16744 T16745 compound United,States
R4913 T16745 T16740 npadvmod States,Sunnyvale
R4914 T16746 T16740 punct ),Sunnyvale
R4915 T16747 T16734 punct .,purchased
R4916 T16924 T16925 compound Western,blot
R4917 T16925 T16926 compound blot,analysis
R4918 T16927 T16926 punct .,analysis
R4919 T16929 T16930 nsubjpass Cells,lysed
R4920 T16931 T16930 auxpass were,lysed
R4921 T16932 T16930 prep in,lysed
R4922 T16933 T16934 compound Laemli,buffer
R4923 T16934 T16932 pobj buffer,in
R4924 T16935 T16930 cc and,lysed
R4925 T16936 T16930 conj run,lysed
R4926 T16937 T16936 prep onto,run
R4927 T16938 T16939 det a,gel
R4928 T16939 T16937 pobj gel,onto
R4929 T16940 T16941 nummod 4,%
R4930 T16941 T16939 nmod %,gel
R4931 T16942 T16943 punct –,%
R4932 T16943 T16941 prep %,%
R4933 T16944 T16943 nummod 12,%
R4934 T16945 T16939 compound SDS,gel
R4935 T16946 T16939 compound PAGE,gel
R4936 T16947 T16939 compound gradient,gel
R4937 T16948 T16930 punct .,lysed
R4938 T16950 T16951 nsubj We,used
R4939 T16952 T16951 dobj antibodies,used
R4940 T16953 T16952 prep against,antibodies
R4941 T16954 T16953 pobj p150CAF,against
R4942 T16955 T16954 punct -,p150CAF
R4943 T16956 T16954 nummod 1,p150CAF
R4944 T16957 T16958 punct (,dilution
R4945 T16958 T16954 parataxis dilution,p150CAF
R4946 T16959 T16958 punct [,dilution
R4947 T16960 T16958 dep 16,dilution
R4948 T16961 T16958 punct ],dilution
R4949 T16962 T16958 punct ;,dilution
R4950 T16963 T16958 npadvmod 1,dilution
R4951 T16964 T16965 punct /,500
R4952 T16965 T16963 prep 500,1
R4953 T16966 T16958 punct ),dilution
R4954 T16967 T16954 punct ", ",p150CAF
R4955 T16968 T16954 conj HP1α,p150CAF
R4956 T16969 T16970 punct (,1
R4957 T16970 T16968 parataxis 1,HP1α
R4958 T16971 T16970 dep 2G9,1
R4959 T16972 T16970 punct ", ",1
R4960 T16973 T16970 dep Euromedex,1
R4961 T16974 T16970 punct ;,1
R4962 T16975 T16976 punct /,500
R4963 T16976 T16970 prep 500,1
R4964 T16977 T16970 punct ),1
R4965 T16978 T16968 punct ", ",HP1α
R4966 T16979 T16980 compound Histone,H3
R4967 T16980 T16968 conj H3,HP1α
R4968 T16981 T16982 punct (,1
R4969 T16982 T16980 parataxis 1,H3
R4970 T16983 T16982 dep Abcam,1
R4971 T16984 T16982 punct ;,1
R4972 T16985 T16986 punct /,500
R4973 T16986 T16982 prep 500,1
R4974 T16987 T16982 punct ),1
R4975 T16988 T16980 punct ", ",H3
R4976 T16989 T16980 conj H3K9me3,H3
R4977 T16990 T16991 punct (,1
R4978 T16991 T16989 parataxis 1,H3K9me3
R4979 T16992 T16991 dep Upstate,1
R4980 T16993 T16991 punct ;,1
R4981 T16994 T16995 punct /,500
R4982 T16995 T16991 prep 500,1
R4983 T16996 T16991 punct ),1
R4984 T16997 T16989 cc and,H3K9me3
R4985 T16998 T16999 compound β,actin
R4986 T16999 T16989 conj actin,H3K9me3
R4987 T17000 T16999 punct -,actin
R4988 T17001 T17002 punct (,1
R4989 T17002 T16999 parataxis 1,actin
R4990 T17003 T17002 dep Upstate,1
R4991 T17004 T17002 punct ;,1
R4992 T17005 T17006 punct /,000
R4993 T17006 T17002 prep 000,1
R4994 T17007 T17006 nummod 40,000
R4995 T17008 T17002 punct ),1
R4996 T17009 T16951 punct .,used
R4997 T17171 T17172 compound DNA,FISH
R4998 T17173 T17172 punct .,FISH
R4999 T17175 T17176 nsubjpass Probes,described
R5000 T17177 T17176 auxpass were,described
R5001 T17178 T17176 advmod previously,described
R5002 T17179 T17180 punct [,20
R5003 T17180 T17176 parataxis 20,described
R5004 T17181 T17180 punct ],20
R5005 T17182 T17176 punct .,described
R5006 T17184 T17185 nmod Biotin,dUTP
R5007 T17185 T17189 npadvmod dUTP,labeled
R5008 T17186 T17185 punct -,dUTP
R5009 T17187 T17185 nummod 16,dUTP
R5010 T17188 T17185 punct -,dUTP
R5011 T17189 T17203 amod labeled,probes
R5012 T17190 T17185 cc or,dUTP
R5013 T17191 T17192 nmod Digoxigenin,dUTP
R5014 T17192 T17185 conj dUTP,dUTP
R5015 T17193 T17192 punct -,dUTP
R5016 T17194 T17192 nummod 11,dUTP
R5017 T17195 T17192 punct -,dUTP
R5018 T17196 T17197 punct (,Roche
R5019 T17197 T17192 parataxis Roche,dUTP
R5020 T17198 T17197 punct ", ",Roche
R5021 T17199 T17197 npadvmod Basel,Roche
R5022 T17200 T17197 punct ", ",Roche
R5023 T17201 T17197 npadvmod Switzerland,Roche
R5024 T17202 T17197 punct ),Roche
R5025 T17203 T17204 nsubjpass probes,generated
R5026 T17205 T17204 auxpass were,generated
R5027 T17206 T17204 prep by,generated
R5028 T17207 T17208 compound nick,translation
R5029 T17208 T17206 pobj translation,by
R5030 T17209 T17210 punct (,Roche
R5031 T17210 T17204 parataxis Roche,generated
R5032 T17211 T17210 punct ),Roche
R5033 T17212 T17204 cc and,generated
R5034 T17213 T17214 nsubj FISH,performed
R5035 T17214 T17204 conj performed,generated
R5036 T17215 T17216 mark as,described
R5037 T17216 T17214 advcl described,performed
R5038 T17217 T17218 punct [,20
R5039 T17218 T17214 parataxis 20,performed
R5040 T17219 T17218 punct ],20
R5041 T17220 T17214 punct .,performed
R5042 T17222 T17223 compound Image,acquisition
R5043 T17223 T17224 nsubjpass acquisition,performed
R5044 T17225 T17224 auxpass was,performed
R5045 T17226 T17224 prep with,performed
R5046 T17227 T17228 det the,microscope
R5047 T17228 T17226 pobj microscope,with
R5048 T17229 T17230 compound Deltavision,RT
R5049 T17230 T17228 compound RT,microscope
R5050 T17231 T17232 punct (,objective
R5051 T17232 T17228 parataxis objective,microscope
R5052 T17233 T17232 dep 100,objective
R5053 T17234 T17233 punct ×,100
R5054 T17235 T17232 punct ", ",objective
R5055 T17236 T17232 nummod 1.4,objective
R5056 T17237 T17232 compound NA,objective
R5057 T17238 T17232 punct ),objective
R5058 T17239 T17224 punct ", ",performed
R5059 T17240 T17241 nsubjpass images,deconvoluted
R5060 T17241 T17224 conj deconvoluted,performed
R5061 T17242 T17241 auxpass were,deconvoluted
R5062 T17243 T17241 punct ", ",deconvoluted
R5063 T17244 T17241 cc and,deconvoluted
R5064 T17245 T17246 compound fluorescence,profiles
R5065 T17246 T17247 nsubj profiles,measured
R5066 T17247 T17241 conj measured,deconvoluted
R5067 T17248 T17247 prep along,measured
R5068 T17249 T17250 det an,line
R5069 T17250 T17248 pobj line,along
R5070 T17251 T17250 amod arbitrary,line
R5071 T17252 T17247 advcl using,measured
R5072 T17253 T17252 dobj SoftWorx,using
R5073 T17254 T17247 punct .,measured
R5074 T17929 T17930 compound Nuclei,preparation
R5075 T17931 T17930 punct ", ",preparation
R5076 T17932 T17933 compound nuclease,digestion
R5077 T17933 T17930 conj digestion,preparation
R5078 T17934 T17933 punct ", ",digestion
R5079 T17935 T17933 cc and,digestion
R5080 T17936 T17937 amod biochemical,analysis
R5081 T17937 T17933 conj analysis,digestion
R5082 T17938 T17937 prep of,analysis
R5083 T17939 T17938 pobj chromatin,of
R5084 T17940 T17937 punct .,analysis
R5085 T17942 T17943 compound ES,cells
R5086 T17943 T17944 nsubjpass cells,incubated
R5087 T17945 T17944 auxpass were,incubated
R5088 T17946 T17944 prep on,incubated
R5089 T17947 T17946 pobj ice,on
R5090 T17948 T17944 prep for,incubated
R5091 T17949 T17950 nummod 10,min
R5092 T17950 T17948 pobj min,for
R5093 T17951 T17944 prep in,incubated
R5094 T17952 T17951 pobj buffer,in
R5095 T17953 T17952 nummod 1,buffer
R5096 T17954 T17955 punct (,HCl
R5097 T17955 T17952 parataxis HCl,buffer
R5098 T17956 T17957 nummod 15,mM
R5099 T17957 T17955 compound mM,HCl
R5100 T17958 T17955 compound Tris,HCl
R5101 T17959 T17955 punct -,HCl
R5102 T17960 T17961 punct [,pH
R5103 T17961 T17955 parataxis pH,HCl
R5104 T17962 T17961 nummod 7.5,pH
R5105 T17963 T17961 punct ],pH
R5106 T17964 T17955 punct ", ",HCl
R5107 T17965 T17966 nummod 0.3,M
R5108 T17966 T17967 compound M,sucrose
R5109 T17967 T17955 appos sucrose,HCl
R5110 T17968 T17955 punct ", ",HCl
R5111 T17969 T17970 nummod 60,mM
R5112 T17970 T17971 compound mM,KCl
R5113 T17971 T17955 appos KCl,HCl
R5114 T17972 T17955 punct ", ",HCl
R5115 T17973 T17974 nummod 15,mM
R5116 T17974 T17975 compound mM,NaCl
R5117 T17975 T17955 appos NaCl,HCl
R5118 T17976 T17955 punct ", ",HCl
R5119 T17977 T17978 nummod 5,mM
R5120 T17978 T17979 compound mM,MgCl2
R5121 T17979 T17955 appos MgCl2,HCl
R5122 T17980 T17955 punct ", ",HCl
R5123 T17981 T17982 nummod 0.1,mM
R5124 T17982 T17983 compound mM,EGTA
R5125 T17983 T17955 appos EGTA,HCl
R5126 T17984 T17955 punct ),HCl
R5127 T17985 T17952 prep with,buffer
R5128 T17986 T17987 nummod 0.15,%
R5129 T17987 T17988 compound %,IGEPAL
R5130 T17988 T17985 pobj IGEPAL,with
R5131 T17989 T17990 punct (,Sigma
R5132 T17990 T17988 parataxis Sigma,IGEPAL
R5133 T17991 T17990 punct ", ",Sigma
R5134 T17992 T17993 compound St.,Louis
R5135 T17993 T17990 npadvmod Louis,Sigma
R5136 T17994 T17990 punct ", ",Sigma
R5137 T17995 T17990 npadvmod Missouri,Sigma
R5138 T17996 T17990 punct ", ",Sigma
R5139 T17997 T17998 compound United,States
R5140 T17998 T17990 npadvmod States,Sigma
R5141 T17999 T17990 punct ),Sigma
R5142 T18000 T17944 punct .,incubated
R5143 T18002 T18003 nsubjpass Nuclei,purified
R5144 T18004 T18003 auxpass were,purified
R5145 T18005 T18003 prep by,purified
R5146 T18006 T18005 pobj centrifugation,by
R5147 T18007 T18008 punct (,g
R5148 T18008 T18006 parataxis g,centrifugation
R5149 T18009 T18008 nummod "10,000",g
R5150 T18010 T18009 punct ×,"10,000"
R5151 T18011 T18008 prep for,g
R5152 T18012 T18013 nummod 30,min
R5153 T18013 T18011 pobj min,for
R5154 T18014 T18008 punct ", ",g
R5155 T18015 T18008 prep at,g
R5156 T18016 T18017 nummod 4,°C
R5157 T18017 T18015 pobj °C,at
R5158 T18018 T18008 punct ),g
R5159 T18019 T18003 prep on,purified
R5160 T18020 T18021 compound sucrose,cushions
R5161 T18021 T18019 pobj cushions,on
R5162 T18022 T18023 punct (,buffer
R5163 T18023 T18021 parataxis buffer,cushions
R5164 T18024 T18023 nummod 1,buffer
R5165 T18025 T18023 prep with,buffer
R5166 T18026 T18027 nummod 1.2,M
R5167 T18027 T18028 compound M,sucrose
R5168 T18028 T18025 pobj sucrose,with
R5169 T18029 T18023 punct ),buffer
R5170 T18030 T18003 cc and,purified
R5171 T18031 T18003 conj resuspended,purified
R5172 T18032 T18031 prep in,resuspended
R5173 T18033 T18034 compound nuclease,buffer
R5174 T18034 T18032 pobj buffer,in
R5175 T18035 T18036 punct (,HCl
R5176 T18036 T18034 parataxis HCl,buffer
R5177 T18037 T18038 nummod 50,mM
R5178 T18038 T18036 compound mM,HCl
R5179 T18039 T18036 compound Tris,HCl
R5180 T18040 T18036 punct -,HCl
R5181 T18041 T18042 punct [,pH
R5182 T18042 T18036 parataxis pH,HCl
R5183 T18043 T18042 nummod 7.5,pH
R5184 T18044 T18042 punct ],pH
R5185 T18045 T18036 punct ", ",HCl
R5186 T18046 T18047 nummod 20,mM
R5187 T18047 T18048 compound mM,NaCl
R5188 T18048 T18036 appos NaCl,HCl
R5189 T18049 T18036 punct ", ",HCl
R5190 T18050 T18051 nummod 0.32,M
R5191 T18051 T18052 compound M,sucrose
R5192 T18052 T18036 appos sucrose,HCl
R5193 T18053 T18036 punct ", ",HCl
R5194 T18054 T18055 nummod 4,mM
R5195 T18055 T18056 compound mM,MgCl2
R5196 T18056 T18036 appos MgCl2,HCl
R5197 T18057 T18036 punct ", ",HCl
R5198 T18058 T18059 nummod 1,mM
R5199 T18059 T18060 compound mM,CaCl2
R5200 T18060 T18036 appos CaCl2,HCl
R5201 T18061 T18036 punct ),HCl
R5202 T18062 T18003 punct .,purified
R5203 T18064 T18065 quantmod About,2.106
R5204 T18065 T18066 nummod 2.106,nuclei
R5205 T18066 T18067 nsubjpass nuclei,incubated
R5206 T18068 T18067 auxpass were,incubated
R5207 T18069 T18067 prep with,incubated
R5208 T18070 T18071 amod increasing,quantities
R5209 T18071 T18069 pobj quantities,with
R5210 T18072 T18071 prep of,quantities
R5211 T18073 T18072 pobj MNase,of
R5212 T18074 T18075 punct (,units
R5213 T18075 T18073 parataxis units,MNase
R5214 T18076 T18077 quantmod 0.04,1.6
R5215 T18077 T18075 nummod 1.6,units
R5216 T18078 T18077 punct –,1.6
R5217 T18079 T18075 punct ),units
R5218 T18080 T18073 cc or,MNase
R5219 T18081 T18073 conj DNase,MNase
R5220 T18082 T18081 nummod I,DNase
R5221 T18083 T18084 punct (,units
R5222 T18084 T18081 parataxis units,DNase
R5223 T18085 T18086 quantmod 0.25,16
R5224 T18086 T18084 nummod 16,units
R5225 T18087 T18086 punct –,16
R5226 T18088 T18084 punct ),units
R5227 T18089 T18067 punct .,incubated
R5228 T18091 T18092 compound Digestion,time
R5229 T18092 T18093 nsubj time,was
R5230 T18094 T18092 prep at,time
R5231 T18095 T18096 nummod 37,°C
R5232 T18096 T18094 pobj °C,at
R5233 T18097 T18098 nummod 10,min
R5234 T18098 T18093 attr min,was
R5235 T18099 T18093 prep for,was
R5236 T18100 T18099 pobj MNase,for
R5237 T18101 T18093 cc and,was
R5238 T18102 T18103 nummod 2,min
R5239 T18103 T18093 conj min,was
R5240 T18104 T18103 prep for,min
R5241 T18105 T18104 pobj DNase,for
R5242 T18106 T18105 nummod I,DNase
R5243 T18107 T18093 punct .,was
R5244 T18109 T18110 nsubjpass Digestions,stopped
R5245 T18111 T18110 auxpass were,stopped
R5246 T18112 T18110 prep by,stopped
R5247 T18113 T18112 pcomp adding,by
R5248 T18114 T18113 dobj SDS,adding
R5249 T18115 T18113 prep to,adding
R5250 T18116 T18117 nummod 1,%
R5251 T18117 T18115 pobj %,to
R5252 T18118 T18113 cc and,adding
R5253 T18119 T18113 conj EDTA,adding
R5254 T18120 T18119 prep to,EDTA
R5255 T18121 T18122 nummod 50,mM
R5256 T18122 T18120 pobj mM,to
R5257 T18123 T18110 punct .,stopped
R5258 T18125 T18126 nsubjpass DNAs,prepared
R5259 T18127 T18126 auxpass were,prepared
R5260 T18128 T18126 prep by,prepared
R5261 T18129 T18130 compound proteinase,K
R5262 T18130 T18131 compound K,digestion
R5263 T18131 T18128 pobj digestion,by
R5264 T18132 T18131 acl followed,digestion
R5265 T18133 T18132 agent by,followed
R5266 T18134 T18135 compound phenol,chloroform
R5267 T18135 T18137 compound chloroform,extraction
R5268 T18136 T18135 punct -,chloroform
R5269 T18137 T18133 pobj extraction,by
R5270 T18138 T18137 cc and,extraction
R5271 T18139 T18140 compound isopropanol,precipitation
R5272 T18140 T18137 conj precipitation,extraction
R5273 T18141 T18126 punct .,prepared
R5274 T18143 T18144 aux To,test
R5275 T18144 T18145 advcl test,incubated
R5276 T18146 T18147 det the,association
R5277 T18147 T18144 dobj association,test
R5278 T18148 T18147 prep of,association
R5279 T18149 T18150 compound histone,H3
R5280 T18150 T18148 pobj H3,of
R5281 T18151 T18147 prep with,association
R5282 T18152 T18151 pobj chromatin,with
R5283 T18153 T18144 prep in,test
R5284 T18154 T18155 nmod control,cells
R5285 T18155 T18153 pobj cells,in
R5286 T18156 T18154 cc and,control
R5287 T18157 T18158 npadvmod p150,depleted
R5288 T18158 T18154 conj depleted,control
R5289 T18159 T18158 punct -,depleted
R5290 T18160 T18145 punct ", ",incubated
R5291 T18161 T18162 amod isolated,nuclei
R5292 T18162 T18145 nsubjpass nuclei,incubated
R5293 T18163 T18145 auxpass were,incubated
R5294 T18164 T18145 prep on,incubated
R5295 T18165 T18164 pobj ice,on
R5296 T18166 T18145 prep for,incubated
R5297 T18167 T18168 nummod 30,min
R5298 T18168 T18166 pobj min,for
R5299 T18169 T18145 prep in,incubated
R5300 T18170 T18169 pobj buffer,in
R5301 T18171 T18170 nummod 2,buffer
R5302 T18172 T18173 punct (,Hepes
R5303 T18173 T18170 parataxis Hepes,buffer
R5304 T18174 T18175 nummod 50,mM
R5305 T18175 T18173 compound mM,Hepes
R5306 T18176 T18177 punct [,pH7.9
R5307 T18177 T18173 parataxis pH7.9,Hepes
R5308 T18178 T18177 punct ],pH7.9
R5309 T18179 T18173 punct ", ",Hepes
R5310 T18180 T18181 nummod 20,%
R5311 T18181 T18182 compound %,Glycerol
R5312 T18182 T18173 appos Glycerol,Hepes
R5313 T18183 T18173 punct ", ",Hepes
R5314 T18184 T18185 nummod 3,mM
R5315 T18185 T18186 compound mM,MgCl2
R5316 T18186 T18173 appos MgCl2,Hepes
R5317 T18187 T18173 punct ", ",Hepes
R5318 T18188 T18189 nummod 0.1,%
R5319 T18189 T18190 compound %,IGEPAL
R5320 T18190 T18173 appos IGEPAL,Hepes
R5321 T18191 T18173 punct ", ",Hepes
R5322 T18192 T18193 nummod 0.5,mM
R5323 T18193 T18194 compound mM,DTT
R5324 T18194 T18173 appos DTT,Hepes
R5325 T18195 T18173 punct ", ",Hepes
R5326 T18196 T18197 nummod 0.5,mM
R5327 T18197 T18198 compound mM,PMSF
R5328 T18198 T18173 appos PMSF,Hepes
R5329 T18199 T18173 punct ),Hepes
R5330 T18200 T18170 acl supplemented,buffer
R5331 T18201 T18200 prep with,supplemented
R5332 T18202 T18203 preconj either,M
R5333 T18203 T18205 nmod M,NaCl
R5334 T18204 T18203 nummod 0.1,M
R5335 T18205 T18201 pobj NaCl,with
R5336 T18206 T18203 punct ", ",M
R5337 T18207 T18208 nummod 0.3,M
R5338 T18208 T18203 conj M,M
R5339 T18209 T18208 punct ", ",M
R5340 T18210 T18211 nummod 0.45,M
R5341 T18211 T18208 conj M,M
R5342 T18212 T18211 punct ", ",M
R5343 T18213 T18214 nummod 0.7,M
R5344 T18214 T18211 conj M,M
R5345 T18215 T18214 punct ", ",M
R5346 T18216 T18214 cc or,M
R5347 T18217 T18218 nummod 1,M
R5348 T18218 T18214 conj M,M
R5349 T18219 T18145 punct .,incubated
R5350 T18221 T18222 prep After,analyzed
R5351 T18223 T18221 pobj centrifugation,After
R5352 T18224 T18223 prep at,centrifugation
R5353 T18225 T18226 nummod "15,000",g
R5354 T18226 T18224 pobj g,at
R5355 T18227 T18222 punct ", ",analyzed
R5356 T18228 T18222 nsubjpass pellets,analyzed
R5357 T18229 T18228 cc and,pellets
R5358 T18230 T18228 conj supernants,pellets
R5359 T18231 T18222 auxpass were,analyzed
R5360 T18232 T18222 prep by,analyzed
R5361 T18233 T18234 compound Western,blot
R5362 T18234 T18232 pobj blot,by
R5363 T18235 T18222 advcl using,analyzed
R5364 T18236 T18237 det a,antibody
R5365 T18237 T18235 dobj antibody,using
R5366 T18238 T18239 compound histone,H3
R5367 T18239 T18237 compound H3,antibody
R5368 T18240 T18241 punct (,Abcam
R5369 T18241 T18237 parataxis Abcam,antibody
R5370 T18242 T18241 punct ),Abcam
R5371 T18243 T18222 punct .,analyzed
R5372 T18632 T18631 punct .,ChIP
R5373 T18634 T18635 nsubj We,prepared
R5374 T18636 T18637 amod native,fragments
R5375 T18637 T18635 dobj fragments,prepared
R5376 T18638 T18637 compound chromatin,fragments
R5377 T18639 T18637 prep of,fragments
R5378 T18640 T18641 quantmod two,six
R5379 T18641 T18643 nummod six,nucleosomes
R5380 T18642 T18641 quantmod to,six
R5381 T18643 T18639 pobj nucleosomes,of
R5382 T18644 T18643 prep in,nucleosomes
R5383 T18645 T18644 pobj length,in
R5384 T18646 T18647 mark as,described
R5385 T18647 T18635 advcl described,prepared
R5386 T18648 T18649 punct [,28
R5387 T18649 T18635 parataxis 28,prepared
R5388 T18650 T18649 punct ],28
R5389 T18651 T18635 punct .,prepared
R5390 T18653 T18654 nummod 5,μg
R5391 T18654 T18655 nsubjpass μg,incubated
R5392 T18656 T18654 prep of,μg
R5393 T18657 T18656 pobj chromatin,of
R5394 T18658 T18655 auxpass were,incubated
R5395 T18659 T18655 advmod overnight,incubated
R5396 T18660 T18655 prep with,incubated
R5397 T18661 T18662 nummod 10,μl
R5398 T18662 T18660 pobj μl,with
R5399 T18663 T18662 prep of,μl
R5400 T18664 T18665 amod commercial,antibodies
R5401 T18665 T18663 pobj antibodies,of
R5402 T18666 T18655 punct .,incubated
R5403 T18668 T18669 prep After,purified
R5404 T18670 T18668 pobj incubation,After
R5405 T18671 T18670 prep with,incubation
R5406 T18672 T18673 compound protein,G
R5407 T18673 T18675 compound G,sepharose
R5408 T18674 T18673 punct -,G
R5409 T18675 T18671 pobj sepharose,with
R5410 T18676 T18670 cc and,incubation
R5411 T18677 T18678 nummod three,washes
R5412 T18678 T18670 conj washes,incubation
R5413 T18679 T18669 punct ", ",purified
R5414 T18680 T18681 amod immunoprecipitated,DNA
R5415 T18681 T18669 nsubjpass DNA,purified
R5416 T18682 T18669 auxpass was,purified
R5417 T18683 T18669 punct ", ",purified
R5418 T18684 T18669 conj sized,purified
R5419 T18685 T18684 prep onto,sized
R5420 T18686 T18687 det an,gel
R5421 T18687 T18685 pobj gel,onto
R5422 T18688 T18687 compound agarose,gel
R5423 T18689 T18684 punct ", ",sized
R5424 T18690 T18684 cc and,sized
R5425 T18691 T18684 conj analyzed,sized
R5426 T18692 T18691 prep by,analyzed
R5427 T18693 T18694 compound Southern,blot
R5428 T18694 T18692 pobj blot,by
R5429 T18695 T18669 punct .,purified
R5430 T18697 T18698 compound Hybridization,signals
R5431 T18698 T18699 nsubjpass signals,quantified
R5432 T18700 T18699 auxpass were,quantified
R5433 T18701 T18699 advcl using,quantified
R5434 T18702 T18703 det an,Imager
R5435 T18703 T18701 dobj Imager,using
R5436 T18704 T18703 compound Instant,Imager
R5437 T18705 T18706 punct (,PerkinElmer
R5438 T18706 T18703 parataxis PerkinElmer,Imager
R5439 T18707 T18706 punct ", ",PerkinElmer
R5440 T18708 T18706 npadvmod Wellesley,PerkinElmer
R5441 T18709 T18706 punct ", ",PerkinElmer
R5442 T18710 T18706 npadvmod California,PerkinElmer
R5443 T18711 T18706 punct ", ",PerkinElmer
R5444 T18712 T18713 compound United,States
R5445 T18713 T18706 npadvmod States,PerkinElmer
R5446 T18714 T18706 punct ),PerkinElmer
R5447 T18715 T18699 punct .,quantified
R5448 T18717 T18718 nsubjpass Antibodies,purchased
R5449 T18719 T18717 amod specific,Antibodies
R5450 T18720 T18717 prep for,Antibodies
R5451 T18721 T18720 pobj H4K20me3,for
R5452 T18722 T18718 auxpass were,purchased
R5453 T18723 T18718 prep from,purchased
R5454 T18724 T18723 pobj Abcam,from
R5455 T18725 T18718 punct .,purchased
R5456 T18727 T18728 nsubjpass Antibodies,purchased
R5457 T18729 T18727 prep for,Antibodies
R5458 T18730 T18729 pobj H3K9me3,for
R5459 T18731 T18728 auxpass were,purchased
R5460 T18732 T18728 prep from,purchased
R5461 T18733 T18734 preconj both,Abcam
R5462 T18734 T18732 pobj Abcam,from
R5463 T18735 T18734 cc and,Abcam
R5464 T18736 T18737 compound Upstate,Biotechnology
R5465 T18737 T18734 conj Biotechnology,Abcam
R5466 T18738 T18728 cc and,purchased
R5467 T18739 T18728 conj gave,purchased
R5468 T18740 T18741 amod similar,results
R5469 T18741 T18739 dobj results,gave
R5470 T18742 T18728 punct .,purchased
R5471 T18744 T18745 prep For,used
R5472 T18746 T18747 compound Southern,blot
R5473 T18747 T18748 compound blot,analysis
R5474 T18748 T18744 pobj analysis,For
R5475 T18749 T18745 punct ", ",used
R5476 T18750 T18745 nsubj we,used
R5477 T18751 T18752 det a,fragment
R5478 T18752 T18745 dobj fragment,used
R5479 T18753 T18754 nummod 240,bp
R5480 T18754 T18752 compound bp,fragment
R5481 T18755 T18754 punct -,bp
R5482 T18756 T18757 compound EcoRI,BamHI
R5483 T18757 T18752 compound BamHI,fragment
R5484 T18758 T18757 punct /,BamHI
R5485 T18759 T18752 prep from,fragment
R5486 T18760 T18759 pobj pSAT,from
R5487 T18761 T18762 punct (,probe
R5488 T18762 T18760 parataxis probe,pSAT
R5489 T18763 T18762 amod major,probe
R5490 T18764 T18762 compound satellite,probe
R5491 T18765 T18762 punct ),probe
R5492 T18766 T18767 punct [,45
R5493 T18767 T18752 parataxis 45,fragment
R5494 T18768 T18769 punct –,47
R5495 T18769 T18767 prep 47,45
R5496 T18770 T18767 punct ],45
R5497 T18771 T18752 cc and,fragment
R5498 T18772 T18773 det a,fragment
R5499 T18773 T18752 conj fragment,fragment
R5500 T18774 T18775 nummod 360,bp
R5501 T18775 T18773 compound bp,fragment
R5502 T18776 T18775 punct -,bp
R5503 T18777 T18778 compound EcoRI,HindIII
R5504 T18778 T18773 compound HindIII,fragment
R5505 T18779 T18778 punct /,HindIII
R5506 T18780 T18773 prep from,fragment
R5507 T18781 T18780 pobj R198,from
R5508 T18782 T18773 punct ", ",fragment
R5509 T18783 T18773 acl corresponding,fragment
R5510 T18784 T18783 prep to,corresponding
R5511 T18785 T18786 nummod three,copies
R5512 T18786 T18784 pobj copies,to
R5513 T18787 T18786 prep of,copies
R5514 T18788 T18789 det the,repeat
R5515 T18789 T18787 pobj repeat,of
R5516 T18790 T18791 nummod 120,bp
R5517 T18791 T18789 nmod bp,repeat
R5518 T18792 T18791 punct -,bp
R5519 T18793 T18794 amod minor,satellite
R5520 T18794 T18789 compound satellite,repeat
R5521 T18795 T18796 punct [,49
R5522 T18796 T18783 parataxis 49,corresponding
R5523 T18797 T18796 punct ],49
R5524 T18798 T18745 punct .,used
R5525 T18800 T18801 nsubjpass Probes,described
R5526 T18802 T18800 acl used,Probes
R5527 T18803 T18802 prep in,used
R5528 T18804 T18805 compound FISH,experiments
R5529 T18805 T18803 pobj experiments,in
R5530 T18806 T18801 auxpass were,described
R5531 T18807 T18801 prep in,described
R5532 T18808 T18807 punct [,in
R5533 T18809 T18807 pobj 46,in
R5534 T18810 T18811 punct –,48
R5535 T18811 T18809 prep 48,46
R5536 T18812 T18801 punct ],described
R5537 T18813 T18801 punct .,described
R5538 T18815 T18816 det The,probe
R5539 T18816 T18817 nsubjpass probe,described
R5540 T18818 T18816 prep for,probe
R5541 T18819 T18820 compound IAP,elements
R5542 T18820 T18818 pobj elements,for
R5543 T18821 T18817 auxpass was,described
R5544 T18822 T18817 prep in,described
R5545 T18823 T18822 punct [,in
R5546 T18824 T18822 pobj 50,in
R5547 T18825 T18817 punct ],described
R5548 T18826 T18817 punct .,described

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T14773 23-36 _FRAGMENT denotes Generation of
T14774 60-67 GO:0009790 denotes embryos
T14775 37-43 PR_EXT:000005402 denotes Chaf1a
T14776 44-50 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14777 51-55 NCBITaxon:10088 denotes mice
T14778 60-67 UBERON:0000922 denotes embryos
T14779 97-104 SO_EXT:0001026 denotes genomic
T14780 125-126 CHEBI_SO_EXT:base denotes b
T14781 133-141 SO:0000357 denotes flanking
T14782 142-148 PR_EXT:000005402 denotes Chaf1a
T14783 149-153 SO_EXT:0000147 denotes exon
T14784 163-166 CHEBI_SO_EXT:DNA denotes DNA
T14785 208-215 SO_EXT:sequence_cloning_process denotes cloning
T14786 225-233 CHEBI:7507 denotes neomycin
T14787 253-258 CHEBI_EXT:27026 denotes toxin
T14788 260-269 SO_EXT:0005853 denotes cassettes
T14789 302-311 SO_EXT:engineered_biological_sequence denotes construct
T14790 316-327 GO:0009294 denotes transfected
T14791 331-339 CL:0002322 denotes ES cells
T14792 334-339 CL_GO_EXT:cell denotes cells
T14793 374-385 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T14794 386-394 CL:0002322 denotes ES cells
T14795 389-394 CL_GO_EXT:cell denotes cells
T14796 408-414 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14797 415-421 PR_EXT:000005402 denotes Chaf1a
T14798 442-448 PR_EXT:000005402 denotes Chaf1a
T14799 470-476 SO_EXT:0001023 denotes allele
T14800 518-524 PR_EXT:000005402 denotes Chaf1a
T14801 525-526 SO_EXT:normal_or_wild_type_or_present denotes +
T14802 527-528 SO_EXT:sequence_nullness_or_absence denotes −
T14803 529-533 NCBITaxon:10088 denotes mice
T14804 547-557 GO_SO_EXT:sequence_rearrangement_process denotes recombined
T14805 558-566 CL:0002322 denotes ES cells
T14806 561-566 CL_GO_EXT:cell denotes cells
T14807 581-592 UBERON:0000358 denotes blastocysts
T14808 597-604 UBERON:0000922 denotes embryos
T14809 637-643 PR_EXT:000005402 denotes Chaf1a
T14810 644-645 SO_EXT:normal_or_wild_type_or_present denotes +
T14811 646-647 SO_EXT:sequence_nullness_or_absence denotes −
T14812 648-652 NCBITaxon:10088 denotes mice
T14813 697-704 UBERON:0000922 denotes Embryos
T14814 738-741 CHEBI:60004 denotes mix
T14815 753-768 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T14816 769-776 SO_EXT:0000112 denotes primers
T14817 818-827 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14818 832-838 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14819 839-846 SO_EXT:0001023 denotes alleles
T14820 974-989 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T14821 990-997 SO_EXT:0000112 denotes primers
T14822 1064-1071 CHEBI:2511 denotes agarose
T14823 1112-1121 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14824 1127-1129 SO_EXT:0000028 denotes bp
T14825 1135-1146 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T14826 1152-1154 SO_EXT:0000028 denotes bp
T14827 1156-1163 SO_EXT:0001023 denotes alleles
T15643 1166-1175 PR_EXT:000005402 denotes P150CAF-1
T15644 1189-1193 GO:0016246 denotes RNAi
T15645 1199-1203 GO:0016246 denotes RNAi
T15646 1204-1218 SO_EXT:0000755 denotes plasmid vector
T15647 1259-1264 NCBITaxon:10088 denotes mouse
T15648 1268-1276 SO_EXT:0000167 denotes promoter
T15649 1283-1292 CHEBI:17939 denotes puromycin
T15650 1303-1307 SO_EXT:0000704 denotes gene
T15651 1393-1399 SO_EXT:0000440 denotes vector
T15652 1419-1423 SO_EXT:0000704 denotes gene
T15653 1455-1460 SO_EXT:0000704 denotes genes
T15654 1464-1472 CL:0002322 denotes ES cells
T15655 1467-1472 CL_GO_EXT:cell denotes cells
T15656 1507-1512 CL_GO_EXT:cell denotes cells
T15657 1589-1594 CL_GO_EXT:cell denotes cells
T15658 1609-1617 SO_EXT:biological_sequence denotes sequence
T15659 1621-1630 PR_EXT:000005402 denotes p150CAF-1
T15660 1631-1636 SO_EXT:0000646 denotes siRNA
T15661 1638-1659 SO_EXT:0000646 denotes short interfering RNA
T15662 1656-1659 CHEBI_SO_EXT:RNA denotes RNA
T15663 1677-1683 SO_EXT:sequence_cloning_process denotes cloned
T15664 1694-1701 SO_EXT:0000155 denotes plasmid
T15665 1715-1721 SO_EXT:0000440 denotes vector
T15666 1722-1731 GO:0010467 denotes expressed
T15667 1734-1739 SO_EXT:0000646 denotes siRNA
T15668 1757-1760 CHEBI_SO_EXT:RNA denotes RNA
T15669 1757-1772 GO:0006401 denotes RNA degradation
T15670 1774-1782 CL:0002322 denotes ES cells
T15671 1777-1782 CL_GO_EXT:cell denotes cells
T15672 1788-1799 GO:0009294 denotes transfected
T15673 1828-1831 CHEBI_SO_EXT:RNA denotes RNA
T15674 1832-1846 SO_EXT:0000755 denotes plasmid vector
T15675 1860-1867 CHEBI:5291 denotes gelatin
T15676 1934-1943 CHEBI:17939 denotes Puromycin
T15677 1990-1995 CL_GO_EXT:cell denotes cells
T15678 2051-2060 PR_EXT:000005402 denotes p150CAF-1
T15679 2061-2065 GO:0016246 denotes RNAi
T15680 2066-2072 SO_EXT:0000440 denotes vector
T15681 2122-2127 GO:0033186 denotes CAF-1
T15682 2146-2151 CL_GO_EXT:cell denotes cells
T15683 2218-2227 PR_EXT:000005402 denotes p150CAF-1
T15684 2228-2238 GO:0010467 denotes expression
T15685 2268-2273 CL_GO_EXT:cell denotes cells
T15686 2305-2309 SO_EXT:0000704 denotes gene
T15687 2349-2360 GO:0009294 denotes transfected
T15688 2381-2385 GO:0016246 denotes RNAi
T15689 2386-2400 SO_EXT:0000755 denotes plasmid vector
T15690 2517-2522 CL_GO_EXT:cell denotes Cells
T15691 2528-2539 GO_EXT:trypsinization denotes trypsinized
T15692 2606-2615 CHEBI:17939 denotes puromycin
T16159 2618-2622 CL_GO_EXT:cell denotes Cell
T16160 2635-2643 CL:0002322 denotes ES cells
T16161 2638-2643 CL_GO_EXT:cell denotes cells
T16162 2685-2689 SO_EXT:0000704 denotes gene
T16163 2706-2714 CL:0002322 denotes ES cells
T16164 2709-2714 CL_GO_EXT:cell denotes cells
T16165 2732-2736 GO:0016246 denotes RNAi
T16166 2766-2770 CL_GO_EXT:cell denotes cell
T16167 2800-2807 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T16168 2808-2812 GO_EXT:breeding denotes bred
T16169 2826-2831 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T16170 2845-2853 CL:0002322 denotes ES cells
T16171 2848-2853 CL_GO_EXT:cell denotes cells
T16172 2928-2935 UBERON:0000922 denotes embryos
T16568 2979-2984 CL_GO_EXT:cell denotes Cells
T16569 3022-3038 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T16570 3107-3114 UBERON:0000922 denotes embryos
T16571 3154-3158 CHEBI_EXT:37527 denotes acid
T16572 3173-3187 UBERON:0000086 denotes zona pellucida
T16573 3273-3281 CL:0002322 denotes ES cells
T16574 3276-3281 CL_GO_EXT:cell denotes cells
T16575 3283-3293 GO:0042571 denotes Antibodies
T16576 3299-3303 PR_EXT:000005086 denotes HP1α
T16577 3388-3392 CHEBI:472552 denotes BrdU
T16578 3435-3439 PR_EXT:000012421 denotes PCNA
T16579 3467-3470 PR_EXT:000026474 denotes PML
T16580 3567-3577 GO:0042571 denotes Antibodies
T16581 3583-3588 NCBITaxon:10088 denotes mouse
T16582 3589-3597 PR_EXT:000005402 denotes p150CAF1
T16583 3612-3619 SO_EXT:0001707 denotes H3K9me3
T16584 3702-3712 GO:0042571 denotes antibodies
T16585 3733-3742 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T16586 3743-3749 CHEBI_SO_EXT:molecular_probe denotes Probes
T16913 3814-3819 CL_GO_EXT:cell denotes Cells
T16914 3825-3830 GO:0019835 denotes lysed
T16915 3841-3847 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T16916 3870-3873 CHEBI:8984 denotes SDS
T16917 3901-3911 GO:0042571 denotes antibodies
T16918 3920-3929 PR_EXT:000005402 denotes p150CAF-1
T16919 3954-3958 PR_EXT:000005086 denotes HP1α
T16920 3984-3991 CHEBI:15358 denotes Histone
T16921 3984-3994 PR_EXT:000027594 denotes Histone H3
T16922 4011-4018 SO_EXT:0001707 denotes H3K9me3
T16923 4040-4047 PR_EXT:000003676 denotes β-actin
T17162 4070-4073 CHEBI_SO_EXT:DNA denotes DNA
T17163 4080-4086 CHEBI_SO_EXT:molecular_probe denotes Probes
T17164 4119-4125 CHEBI:15956 denotes Biotin
T17165 4129-4133 CHEBI_EXT:dUTP denotes dUTP
T17166 4137-4148 CHEBI:42098 denotes Digoxigenin
T17167 4152-4156 CHEBI_EXT:dUTP denotes dUTP
T17168 4185-4192 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T17169 4193-4199 CHEBI_SO_EXT:molecular_probe denotes probes
T17170 4223-4234 GO:0006412 denotes translation
T17877 4476-4482 GO:0005634 denotes Nuclei
T17878 4496-4504 GO_EXT:0004518 denotes nuclease
T17879 4520-4531 CHEBI_EXT:biochemical denotes biochemical
T17880 4544-4553 GO:0000785 denotes chromatin
T17881 4555-4563 CL:0002322 denotes ES cells
T17882 4558-4563 CL_GO_EXT:cell denotes cells
T17883 4600-4606 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T17884 4616-4620 CHEBI:9754 denotes Tris
T17885 4621-4624 CHEBI:17883 denotes HCl
T17886 4641-4648 CHEBI:17992 denotes sucrose
T17887 4656-4659 CHEBI:32588 denotes KCl
T17888 4667-4671 CHEBI:26710 denotes NaCl
T17889 4678-4683 CHEBI:6636 denotes MgCl2
T17890 4692-4696 CHEBI_EXT:EGTA denotes EGTA
T17891 4761-4767 GO:0005634 denotes Nuclei
T17892 4836-4843 CHEBI:17992 denotes sucrose
T17893 4854-4860 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T17894 4874-4881 CHEBI:17992 denotes sucrose
T17895 4902-4910 GO_EXT:0004518 denotes nuclease
T17896 4911-4917 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T17897 4925-4929 CHEBI:9754 denotes Tris
T17898 4930-4933 CHEBI:17883 denotes HCl
T17899 4950-4954 CHEBI:26710 denotes NaCl
T17900 4963-4970 CHEBI:17992 denotes sucrose
T17901 4977-4982 CHEBI:6636 denotes MgCl2
T17902 4989-4994 CHEBI:3312 denotes CaCl2
T17903 5009-5015 GO:0005634 denotes nuclei
T17904 5087-5094 GO_PR_EXT:deoxyribonuclease_I denotes DNase I
T17905 5171-5178 GO_PR_EXT:deoxyribonuclease_I denotes DNase I
T17906 5214-5217 CHEBI:8984 denotes SDS
T17907 5228-5232 CHEBI_EXT:EDTA denotes EDTA
T17908 5243-5247 CHEBI_SO_EXT:DNA denotes DNAs
T17909 5265-5275 GO_EXT:0008233 denotes proteinase
T17910 5300-5306 CHEBI:15882 denotes phenol
T17911 5307-5317 CHEBI:35255 denotes chloroform
T17912 5333-5344 CHEBI:17824 denotes isopropanol
T17913 5387-5394 CHEBI:15358 denotes histone
T17914 5387-5397 PR_EXT:000027594 denotes histone H3
T17915 5403-5412 GO:0000785 denotes chromatin
T17916 5428-5432 PR_EXT:000005402 denotes p150
T17917 5442-5447 CL_GO_EXT:cell denotes cells
T17918 5458-5464 GO:0005634 denotes nuclei
T17919 5501-5507 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T17920 5517-5522 CHEBI:46756 denotes Hepes
T17921 5536-5544 CHEBI:17754 denotes Glycerol
T17922 5551-5556 CHEBI:6636 denotes MgCl2
T17923 5578-5581 CHEBI:18320 denotes DTT
T17924 5590-5594 CHEBI:8102 denotes PMSF
T17925 5657-5661 CHEBI:26710 denotes NaCl
T17926 5758-5765 CHEBI:15358 denotes histone
T17927 5758-5768 PR_EXT:000027594 denotes histone H3
T17928 5769-5777 GO:0042571 denotes antibody
T18609 5813-5822 GO:0000785 denotes chromatin
T18610 5847-5858 GO:0000786 denotes nucleosomes
T18611 5896-5905 GO:0000785 denotes chromatin
T18612 5956-5966 GO:0042571 denotes antibodies
T18613 5990-5997 CHEBI_PR_EXT:protein denotes protein
T18614 6047-6050 CHEBI_SO_EXT:DNA denotes DNA
T18615 6079-6086 CHEBI:2511 denotes agarose
T18616 6123-6136 GO:0097617 denotes Hybridization
T18617 6238-6248 GO:0042571 denotes Antibodies
T18618 6298-6308 GO:0042571 denotes Antibodies
T18619 6313-6320 SO_EXT:0001707 denotes H3K9me3
T18620 6446-6448 SO_EXT:0000028 denotes bp
T18621 6455-6460 PR_EXT:P23940 denotes BamHI
T18622 6487-6496 SO_EXT:satellite_unit_or_region denotes satellite
T18623 6497-6502 CHEBI_SO_EXT:molecular_probe denotes probe
T18624 6522-6524 SO_EXT:0000028 denotes bp
T18625 6531-6538 PR_EXT:P43870 denotes HindIII
T18626 6582-6588 SO_EXT:sequence_copy_entity denotes copies
T18627 6600-6602 SO_EXT:0000028 denotes bp
T18628 6609-6625 SO_EXT:satellite_unit_or_region denotes satellite repeat
T18629 6632-6638 CHEBI_SO_EXT:molecular_probe denotes Probes
T18630 6695-6700 CHEBI_SO_EXT:molecular_probe denotes probe
R4194 T14774 T14773 _lexicallyChainedTo embryos,Generation of

2_test

Id Subject Object Predicate Lexical cue
17083276-15306854-85837850 1669-1671 15306854 denotes 16
17083276-12529396-85837851 2645-2647 12529396 denotes 42
17083276-11236671-85837852 2953-2955 11236671 denotes 43
17083276-11175742-85837853 3099-3101 11175742 denotes 44
17083276-15306854-85837854 3599-3601 15306854 denotes 16
17083276-15306854-85837855 3932-3934 15306854 denotes 16
17083276-15302854-85837856 4114-4116 15302854 denotes 20
17083276-15302854-85837857 4276-4278 15302854 denotes 20
17083276-15273407-85837858 5883-5885 15273407 denotes 28
17083276-11593003-85837859 6505-6507 11593003 denotes 45
17083276-12867029-85837859 6505-6507 12867029 denotes 45
17083276-1606614-85837859 6505-6507 1606614 denotes 45
17083276-8013255-85837860 6627-6629 8013255 denotes 49
17083276-12867029-85837861 6683-6685 12867029 denotes 46
17083276-1606614-85837861 6683-6685 1606614 denotes 46
17083276-6694730-85837861 6683-6685 6694730 denotes 46
17083276-9771701-85837862 6736-6738 9771701 denotes 50
T36041 1669-1671 15306854 denotes 16
T46532 2645-2647 12529396 denotes 42
T26707 2953-2955 11236671 denotes 43
T3704 3099-3101 11175742 denotes 44
T95429 3599-3601 15306854 denotes 16
T63338 3932-3934 15306854 denotes 16
T22708 4114-4116 15302854 denotes 20
T71895 4276-4278 15302854 denotes 20
T79362 5883-5885 15273407 denotes 28
T43192 6505-6507 11593003 denotes 45
T47693 6505-6507 12867029 denotes 45
T79851 6505-6507 1606614 denotes 45
T53573 6627-6629 8013255 denotes 49
T63565 6683-6685 12867029 denotes 46
T20351 6683-6685 1606614 denotes 46
T664 6683-6685 6694730 denotes 46
T4099 6736-6738 9771701 denotes 50

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T14739 23-36 _FRAGMENT denotes Generation of
T14740 60-67 GO:0009790 denotes embryos
T14741 37-43 PR:000005402 denotes Chaf1a
T14742 51-55 NCBITaxon:10088 denotes mice
T14743 60-67 UBERON:0000922 denotes embryos
T14744 97-104 SO:0001026 denotes genomic
T14745 133-141 SO:0000357 denotes flanking
T14746 142-148 PR:000005402 denotes Chaf1a
T14747 149-153 SO:0000147 denotes exon
T14748 225-233 CHEBI:7507 denotes neomycin
T14749 253-258 CHEBI:27026 denotes toxin
T14750 260-269 SO:0005853 denotes cassettes
T14751 316-327 GO:0009294 denotes transfected
T14752 331-339 CL:0002322 denotes ES cells
T14753 386-394 CL:0002322 denotes ES cells
T14754 415-421 PR:000005402 denotes Chaf1a
T14755 442-448 PR:000005402 denotes Chaf1a
T14756 470-476 SO:0001023 denotes allele
T14757 518-524 PR:000005402 denotes Chaf1a
T14758 529-533 NCBITaxon:10088 denotes mice
T14759 558-566 CL:0002322 denotes ES cells
T14760 581-592 UBERON:0000358 denotes blastocysts
T14761 597-604 UBERON:0000922 denotes embryos
T14762 637-643 PR:000005402 denotes Chaf1a
T14763 648-652 NCBITaxon:10088 denotes mice
T14764 697-704 UBERON:0000922 denotes Embryos
T14765 738-741 CHEBI:60004 denotes mix
T14766 769-776 SO:0000112 denotes primers
T14767 839-846 SO:0001023 denotes alleles
T14768 990-997 SO:0000112 denotes primers
T14769 1064-1071 CHEBI:2511 denotes agarose
T14770 1127-1129 SO:0000028 denotes bp
T14771 1152-1154 SO:0000028 denotes bp
T14772 1156-1163 SO:0001023 denotes alleles
T15607 1166-1175 PR:000005402 denotes P150CAF-1
T15608 1189-1193 GO:0016246 denotes RNAi
T15609 1199-1203 GO:0016246 denotes RNAi
T15610 1204-1218 SO:0000755 denotes plasmid vector
T15611 1259-1264 NCBITaxon:10088 denotes mouse
T15612 1268-1276 SO:0000167 denotes promoter
T15613 1283-1292 CHEBI:17939 denotes puromycin
T15614 1303-1307 SO:0000704 denotes gene
T15615 1393-1399 SO:0000440 denotes vector
T15616 1419-1423 SO:0000704 denotes gene
T15617 1455-1460 SO:0000704 denotes genes
T15618 1464-1472 CL:0002322 denotes ES cells
T15619 1621-1630 PR:000005402 denotes p150CAF-1
T15620 1631-1636 SO:0000646 denotes siRNA
T15621 1638-1659 SO:0000646 denotes short interfering RNA
T15622 1694-1701 SO:0000155 denotes plasmid
T15623 1715-1721 SO:0000440 denotes vector
T15624 1722-1731 GO:0010467 denotes expressed
T15625 1734-1739 SO:0000646 denotes siRNA
T15626 1757-1772 GO:0006401 denotes RNA degradation
T15627 1774-1782 CL:0002322 denotes ES cells
T15628 1788-1799 GO:0009294 denotes transfected
T15629 1832-1846 SO:0000755 denotes plasmid vector
T15630 1860-1867 CHEBI:5291 denotes gelatin
T15631 1934-1943 CHEBI:17939 denotes Puromycin
T15632 2051-2060 PR:000005402 denotes p150CAF-1
T15633 2061-2065 GO:0016246 denotes RNAi
T15634 2066-2072 SO:0000440 denotes vector
T15635 2122-2127 GO:0033186 denotes CAF-1
T15636 2218-2227 PR:000005402 denotes p150CAF-1
T15637 2228-2238 GO:0010467 denotes expression
T15638 2305-2309 SO:0000704 denotes gene
T15639 2349-2360 GO:0009294 denotes transfected
T15640 2381-2385 GO:0016246 denotes RNAi
T15641 2386-2400 SO:0000755 denotes plasmid vector
T15642 2606-2615 CHEBI:17939 denotes puromycin
T16153 2635-2643 CL:0002322 denotes ES cells
T16154 2685-2689 SO:0000704 denotes gene
T16155 2706-2714 CL:0002322 denotes ES cells
T16156 2732-2736 GO:0016246 denotes RNAi
T16157 2845-2853 CL:0002322 denotes ES cells
T16158 2928-2935 UBERON:0000922 denotes embryos
T16554 3107-3114 UBERON:0000922 denotes embryos
T16555 3154-3158 CHEBI:37527 denotes acid
T16556 3173-3187 UBERON:0000086 denotes zona pellucida
T16557 3273-3281 CL:0002322 denotes ES cells
T16558 3283-3293 GO:0042571 denotes Antibodies
T16559 3299-3303 PR:000005086 denotes HP1α
T16560 3388-3392 CHEBI:472552 denotes BrdU
T16561 3435-3439 PR:000012421 denotes PCNA
T16562 3467-3470 PR:000026474 denotes PML
T16563 3567-3577 GO:0042571 denotes Antibodies
T16564 3583-3588 NCBITaxon:10088 denotes mouse
T16565 3589-3597 PR:000005402 denotes p150CAF1
T16566 3612-3619 SO:0001707 denotes H3K9me3
T16567 3702-3712 GO:0042571 denotes antibodies
T16904 3825-3830 GO:0019835 denotes lysed
T16905 3870-3873 CHEBI:8984 denotes SDS
T16906 3901-3911 GO:0042571 denotes antibodies
T16907 3920-3929 PR:000005402 denotes p150CAF-1
T16908 3954-3958 PR:000005086 denotes HP1α
T16909 3984-3991 CHEBI:15358 denotes Histone
T16910 3984-3994 PR:000027594 denotes Histone H3
T16911 4011-4018 SO:0001707 denotes H3K9me3
T16912 4040-4047 PR:000003676 denotes β-actin
T17159 4119-4125 CHEBI:15956 denotes Biotin
T17160 4137-4148 CHEBI:42098 denotes Digoxigenin
T17161 4223-4234 GO:0006412 denotes translation
T17840 4476-4482 GO:0005634 denotes Nuclei
T17841 4544-4553 GO:0000785 denotes chromatin
T17842 4555-4563 CL:0002322 denotes ES cells
T17843 4616-4620 CHEBI:9754 denotes Tris
T17844 4621-4624 CHEBI:17883 denotes HCl
T17845 4641-4648 CHEBI:17992 denotes sucrose
T17846 4656-4659 CHEBI:32588 denotes KCl
T17847 4667-4671 CHEBI:26710 denotes NaCl
T17848 4678-4683 CHEBI:6636 denotes MgCl2
T17849 4761-4767 GO:0005634 denotes Nuclei
T17850 4836-4843 CHEBI:17992 denotes sucrose
T17851 4874-4881 CHEBI:17992 denotes sucrose
T17852 4925-4929 CHEBI:9754 denotes Tris
T17853 4930-4933 CHEBI:17883 denotes HCl
T17854 4950-4954 CHEBI:26710 denotes NaCl
T17855 4963-4970 CHEBI:17992 denotes sucrose
T17856 4977-4982 CHEBI:6636 denotes MgCl2
T17857 4989-4994 CHEBI:3312 denotes CaCl2
T17858 5009-5015 GO:0005634 denotes nuclei
T17859 5214-5217 CHEBI:8984 denotes SDS
T17860 5300-5306 CHEBI:15882 denotes phenol
T17861 5307-5317 CHEBI:35255 denotes chloroform
T17862 5333-5344 CHEBI:17824 denotes isopropanol
T17863 5387-5394 CHEBI:15358 denotes histone
T17864 5387-5397 PR:000027594 denotes histone H3
T17865 5403-5412 GO:0000785 denotes chromatin
T17866 5428-5432 PR:000005402 denotes p150
T17867 5458-5464 GO:0005634 denotes nuclei
T17868 5517-5522 CHEBI:46756 denotes Hepes
T17869 5536-5544 CHEBI:17754 denotes Glycerol
T17870 5551-5556 CHEBI:6636 denotes MgCl2
T17871 5578-5581 CHEBI:18320 denotes DTT
T17872 5590-5594 CHEBI:8102 denotes PMSF
T17873 5657-5661 CHEBI:26710 denotes NaCl
T17874 5758-5765 CHEBI:15358 denotes histone
T17875 5758-5768 PR:000027594 denotes histone H3
T17876 5769-5777 GO:0042571 denotes antibody
T18595 5813-5822 GO:0000785 denotes chromatin
T18596 5847-5858 GO:0000786 denotes nucleosomes
T18597 5896-5905 GO:0000785 denotes chromatin
T18598 5956-5966 GO:0042571 denotes antibodies
T18599 6079-6086 CHEBI:2511 denotes agarose
T18600 6123-6136 GO:0097617 denotes Hybridization
T18601 6238-6248 GO:0042571 denotes Antibodies
T18602 6298-6308 GO:0042571 denotes Antibodies
T18603 6313-6320 SO:0001707 denotes H3K9me3
T18604 6446-6448 SO:0000028 denotes bp
T18605 6455-6460 PR:P23940 denotes BamHI
T18606 6522-6524 SO:0000028 denotes bp
T18607 6531-6538 PR:P43870 denotes HindIII
T18608 6600-6602 SO:0000028 denotes bp
R4193 T14740 T14739 _lexicallyChainedTo embryos,Generation of