PMC:1630711 / 22402-22940 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/1630711","sourcedb":"PMC","sourceid":"1630711","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1630711","text":"Figure 5 Nucleosomal Organization Is Not Altered in p150CAF-1-Depleted ES Cells\nNuclei were prepared from ES cells transfected with control or p150CAF-1 siRNA vector. Nuclei were digested with increasing amounts of DNase I or MNase. (A) After digestion with the indicated nucleases, total DNA was prepared and run onto an agarose gel which was stained with ethidium bromide to reveal bulk genomic DNA.\n(B) The DNA was blotted onto a nylon membrane, which was then hybridized with the α-32P-labeled pSAT major satellite repeat probe [47].","divisions":[{"label":"label","span":{"begin":0,"end":8}},{"label":"title","span":{"begin":10,"end":80}},{"label":"p","span":{"begin":81,"end":402}}],"tracks":[{"project":"2_test","denotations":[{"id":"17083276-1606614-85837817","span":{"begin":534,"end":536},"obj":"1606614"},{"id":"T60830","span":{"begin":534,"end":536},"obj":"1606614"}],"attributes":[{"subj":"17083276-1606614-85837817","pred":"source","obj":"2_test"},{"subj":"T60830","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ecd4","default":true}]}]}}