PMC:1630711 / 19600-21321
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/1630711","sourcedb":"PMC","sourceid":"1630711","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1630711","text":"Figure 4 Depletion of p150CAF-1 Leads to Loss of Clustering, Altered Localization, and Decondensation of Pericentric Heterochromatin Domains\nDistribution of pericentric (red) and centric (green) domains was analyzed in the interphase nuclei of mouse ES cells by DNA FISH, using major satellite (pSAT) [47] and minor satellite (pMR150) [48] DNA probes, respectively. (A) In ES cells expressing control (cont) siRNA, pericentric regions from several chromosomes associate in clusters (red). These chromocenters form foci as revealed by DAPI staining (left-hand image), while centric regions (green) remain independent entities at the periphery of these domains. The right-hand image shows the merge between the pericentric and centric FISH signals.\n(B) The organization of pericentric domains was altered in cells expressing p150CAF-1 siRNA. Instead of forming well-defined chromocenters, pericentric domains were found either isolated or associated in heterogeneous aggregates of various sizes, often at the nuclear periphery. Scale bar = 10 μm.\n(C) Control ES cells. Fluorescence was quantified along a line randomly drawn across the nucleus in the merged image and data were plotted. One can distinguish clear peaks corresponding to chromocenters (red) and the condensed minor satellites (green).\n(D) ES cells expressing p150CAF-1 siRNA. p150CAF-1 depletion led to a lower fluorescence intensity and a broader distribution of signals corresponding to DAPI (blue) and major satellite hybridization (red, plot) while the organization of the minor satellites remained unaffected. Insets in the right-hand images show a typical chromocenter in control cells (C) and a disrupted chromocenter in p150CAF-1-depleted cells (D).","divisions":[{"label":"label","span":{"begin":0,"end":8}},{"label":"title","span":{"begin":10,"end":141}},{"label":"p","span":{"begin":142,"end":747}},{"label":"p","span":{"begin":748,"end":1045}},{"label":"p","span":{"begin":1046,"end":1298}}],"tracks":[{"project":"2_test","denotations":[{"id":"17083276-1606614-85837813","span":{"begin":303,"end":305},"obj":"1606614"},{"id":"17083276-6694730-85837814","span":{"begin":337,"end":339},"obj":"6694730"},{"id":"T91663","span":{"begin":303,"end":305},"obj":"1606614"},{"id":"T5011","span":{"begin":337,"end":339},"obj":"6694730"}],"attributes":[{"subj":"17083276-1606614-85837813","pred":"source","obj":"2_test"},{"subj":"17083276-6694730-85837814","pred":"source","obj":"2_test"},{"subj":"T91663","pred":"source","obj":"2_test"},{"subj":"T5011","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#eccf93","default":true}]}]}}