PMC:1630711 / 16580-17252 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T9337","span":{"begin":23,"end":28},"obj":"NN"},{"id":"T9339","span":{"begin":12,"end":16},"obj":"NN"},{"id":"T9340","span":{"begin":1,"end":11},"obj":"JJ"},{"id":"T9341","span":{"begin":16,"end":18},"obj":","},{"id":"T9342","span":{"begin":18,"end":22},"obj":"NN"},{"id":"T9343","span":{"begin":29,"end":32},"obj":"CC"},{"id":"T9344","span":{"begin":33,"end":42},"obj":"NN"},{"id":"T9345","span":{"begin":42,"end":43},"obj":"."}],"relations":[{"id":"R2082","pred":"amod","subj":"T9340","obj":"T9339"},{"id":"R2083","pred":"punct","subj":"T9341","obj":"T9337"},{"id":"R2084","pred":"compound","subj":"T9342","obj":"T9337"},{"id":"R2086","pred":"cc","subj":"T9343","obj":"T9337"},{"id":"R2087","pred":"conj","subj":"T9344","obj":"T9337"}],"text":" subsequent step, cell cycle and viability.\nFigure 3 p150CAF-1 Depletion and Loss of Heterochromatin Organization Are Compatible with Active DNA Replication in ES Cells\n(A) ES cells were transfected with p150CAF-1 or control siRNA vectors. After 3 d under puromycin selection, cells were pulse-labeled for 10 min with BrdU and immediately analyzed by IF. No significant difference in BrdU incorporation could be detected between control and p150CAF-1-depleted cells. The right-hand image shows the merge between the p150CAF-1 and BrdU fluorescence. Scale bar = 10 μm.\n(B) Immunodetection of PCNA (red) revealed no significant difference in the formation of replication fo"}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7816","span":{"begin":18,"end":22},"obj":"CL_GO_EXT:cell"},{"id":"T7817","span":{"begin":18,"end":28},"obj":"GO:0007049"}],"text":" subsequent step, cell cycle and viability.\nFigure 3 p150CAF-1 Depletion and Loss of Heterochromatin Organization Are Compatible with Active DNA Replication in ES Cells\n(A) ES cells were transfected with p150CAF-1 or control siRNA vectors. After 3 d under puromycin selection, cells were pulse-labeled for 10 min with BrdU and immediately analyzed by IF. No significant difference in BrdU incorporation could be detected between control and p150CAF-1-depleted cells. The right-hand image shows the merge between the p150CAF-1 and BrdU fluorescence. Scale bar = 10 μm.\n(B) Immunodetection of PCNA (red) revealed no significant difference in the formation of replication fo"}

{"project":"craft-ca-core-dev","denotations":[{"id":"T7444","span":{"begin":18,"end":28},"obj":"GO:0007049"}],"text":" subsequent step, cell cycle and viability.\nFigure 3 p150CAF-1 Depletion and Loss of Heterochromatin Organization Are Compatible with Active DNA Replication in ES Cells\n(A) ES cells were transfected with p150CAF-1 or control siRNA vectors. After 3 d under puromycin selection, cells were pulse-labeled for 10 min with BrdU and immediately analyzed by IF. No significant difference in BrdU incorporation could be detected between control and p150CAF-1-depleted cells. The right-hand image shows the merge between the p150CAF-1 and BrdU fluorescence. Scale bar = 10 μm.\n(B) Immunodetection of PCNA (red) revealed no significant difference in the formation of replication fo"}