PMC:1630711 / 13421-14682 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1630711","sourcedb":"PMC","sourceid":"1630711","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1630711","text":"Depletion of p150CAF-1 in ES Cells Results in a Severe Alteration of Heterochromatin Organization\n(A) Strategy used to deplete p150CAF-1 by RNAi in ES cells. The siRNA expression vector includes a puromycin selection cassette (Puro), the mouse H1 promoter, and the siRNA encoding sequence. ES cells were kept under puromycin selection during 48 h following transfection.\n(B) Abnormal heterochromatin organization in p150CAF-1-depleted ES cells. Immunodetection of p150CAF-1 (green) and HP1α (red) in ES cells transfected with control (cont) and p150CAF-1 siRNA. siRNA expression results in efficient p150CAF-1 depletion. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue. Scale bar = 10 μm.\n(C) Heterochromatin organization is not altered in p150CAF-1-depleted MEFs. Immunodetection of p150CAF-1 (green) and HP1α (red) in MEFs transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue.\n(D) PML bodies are not altered in p150CAF-1-depleted ES cells. Immunodetection of PML (red) in ES cells transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between PML fluorescence and DAPI-stained DNA in blue.","divisions":[{"label":"title","span":{"begin":0,"end":97}},{"label":"p","span":{"begin":98,"end":370}},{"label":"p","span":{"begin":371,"end":732}},{"label":"p","span":{"begin":733,"end":1013}}],"tracks":[]}