PMC:1630711 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T808	6-8	VBZ	denotes	Is
T809	0-82	sentence	denotes	CAF-1 Is Essential for Heterochromatin Organization in Pluripotent Embryonic Cells
T810	3-4	HYPH	denotes	-
T811	4-5	CD	denotes	1
T812	9-18	JJ	denotes	Essential
T813	19-22	IN	denotes	for
T814	23-38	NN	denotes	Heterochromatin
T815	39-51	NN	denotes	Organization
T816	52-54	IN	denotes	in
T817	55-66	JJ	denotes	Pluripotent
T818	77-82	NNS	denotes	Cells
T819	67-76	JJ	denotes	Embryonic
T820	82-83	sentence	denotes
T821	129-240	sentence	denotes	During mammalian development, chromatin dynamics and epigenetic marking are important for genome reprogramming.
T822	129-135	IN	denotes	During
T823	201-204	VBP	denotes	are
T824	136-145	JJ	denotes	mammalian
T825	146-157	NN	denotes	development
T826	157-159	,	denotes	,
T827	159-168	NN	denotes	chromatin
T828	169-177	NNS	denotes	dynamics
T829	178-181	CC	denotes	and
T830	182-192	JJ	denotes	epigenetic
T831	193-200	NN	denotes	marking
T832	205-214	JJ	denotes	important
T833	215-218	IN	denotes	for
T834	219-225	NN	denotes	genome
T835	226-239	NN	denotes	reprogramming
T836	239-240	.	denotes	.
T837	240-332	sentence	denotes	Recent data suggest an important role for the chromatin assembly machinery in this process.
T838	241-247	JJ	denotes	Recent
T839	248-252	NNS	denotes	data
T840	253-260	VBP	denotes	suggest
T841	261-263	DT	denotes	an
T842	274-278	NN	denotes	role
T843	264-273	JJ	denotes	important
T844	279-282	IN	denotes	for
T845	283-286	DT	denotes	the
T846	306-315	NN	denotes	machinery
T847	287-296	NN	denotes	chromatin
T848	297-305	NN	denotes	assembly
T849	316-318	IN	denotes	in
T850	319-323	DT	denotes	this
T851	324-331	NN	denotes	process
T852	331-332	.	denotes	.
T853	332-534	sentence	denotes	To analyze the role of chromatin assembly factor 1 (CAF-1) during pre-implantation development, we generated a mouse line carrying a targeted mutation in the gene encoding its large subunit, p150CAF-1.
T854	333-335	TO	denotes	To
T855	336-343	VB	denotes	analyze
T856	432-441	VBD	denotes	generated
T857	344-347	DT	denotes	the
T858	348-352	NN	denotes	role
T859	353-355	IN	denotes	of
T860	356-365	NN	denotes	chromatin
T861	366-374	NN	denotes	assembly
T862	375-381	NN	denotes	factor
T863	382-383	CD	denotes	1
T864	384-385	-LRB-	denotes	(
T865	385-388	NN	denotes	CAF
T866	388-389	HYPH	denotes	-
T867	389-390	CD	denotes	1
T868	390-391	-RRB-	denotes	)
T869	392-398	IN	denotes	during
T870	399-415	NN	denotes	pre-implantation
T871	416-427	NN	denotes	development
T872	427-429	,	denotes	,
T873	429-431	PRP	denotes	we
T874	442-443	DT	denotes	a
T875	450-454	NN	denotes	line
T876	444-449	NN	denotes	mouse
T877	455-463	VBG	denotes	carrying
T878	464-465	DT	denotes	a
T879	475-483	NN	denotes	mutation
T880	466-474	VBN	denotes	targeted
T881	484-486	IN	denotes	in
T882	487-490	DT	denotes	the
T883	491-495	NN	denotes	gene
T884	496-504	VBG	denotes	encoding
T885	505-508	PRP$	denotes	its
T886	515-522	NN	denotes	subunit
T887	509-514	JJ	denotes	large
T888	522-524	,	denotes	,
T889	524-531	NN	denotes	p150CAF
T890	531-532	HYPH	denotes	-
T891	532-533	CD	denotes	1
T892	533-534	.	denotes	.
T893	534-626	sentence	denotes	Loss of p150CAF-1 in homozygous mutants leads to developmental arrest at the 16-cell stage.
T894	535-539	NN	denotes	Loss
T895	575-580	VBZ	denotes	leads
T896	540-542	IN	denotes	of
T897	543-550	NN	denotes	p150CAF
T898	550-551	HYPH	denotes	-
T899	551-552	CD	denotes	1
T900	553-555	IN	denotes	in
T901	556-566	JJ	denotes	homozygous
T902	567-574	NNS	denotes	mutants
T903	581-583	IN	denotes	to
T904	584-597	JJ	denotes	developmental
T905	598-604	NN	denotes	arrest
T906	605-607	IN	denotes	at
T907	608-611	DT	denotes	the
T908	620-625	NN	denotes	stage
T909	612-614	CD	denotes	16
T910	615-619	NN	denotes	cell
T911	614-615	HYPH	denotes	-
T912	625-626	.	denotes	.
T913	626-755	sentence	denotes	Absence of p150CAF-1 in these embryos results in severe alterations in the nuclear organization of constitutive heterochromatin.
T914	627-634	NN	denotes	Absence
T915	665-672	VBZ	denotes	results
T916	635-637	IN	denotes	of
T917	638-645	NN	denotes	p150CAF
T918	645-646	HYPH	denotes	-
T919	646-647	CD	denotes	1
T920	648-650	IN	denotes	in
T921	651-656	DT	denotes	these
T922	657-664	NNS	denotes	embryos
T923	673-675	IN	denotes	in
T924	676-682	JJ	denotes	severe
T925	683-694	NNS	denotes	alterations
T926	695-697	IN	denotes	in
T927	698-701	DT	denotes	the
T928	710-722	NN	denotes	organization
T929	702-709	JJ	denotes	nuclear
T930	723-725	IN	denotes	of
T931	726-738	JJ	denotes	constitutive
T932	739-754	NN	denotes	heterochromatin
T933	754-755	.	denotes	.
T934	755-898	sentence	denotes	We provide evidence that in wild-type embryos, heterochromatin domains are extensively reorganized between the two-cell and blastocyst stages.
T935	756-758	PRP	denotes	We
T936	759-766	VBP	denotes	provide
T937	767-775	NN	denotes	evidence
T938	776-780	IN	denotes	that
T939	843-854	VBN	denotes	reorganized
T940	781-783	IN	denotes	in
T941	784-788	JJ	denotes	wild
T942	789-793	NN	denotes	type
T943	788-789	HYPH	denotes	-
T944	794-801	NNS	denotes	embryos
T945	801-803	,	denotes	,
T946	803-818	NN	denotes	heterochromatin
T947	819-826	NNS	denotes	domains
T948	827-830	VBP	denotes	are
T949	831-842	RB	denotes	extensively
T950	855-862	IN	denotes	between
T951	863-866	DT	denotes	the
T952	891-897	NNS	denotes	stages
T953	867-870	CD	denotes	two
T954	871-875	NN	denotes	cell
T955	870-871	HYPH	denotes	-
T956	876-879	CC	denotes	and
T957	880-890	NN	denotes	blastocyst
T958	897-898	.	denotes	.
T959	898-1197	sentence	denotes	In p150CAF-1 mutant 16-cell stage embryos, the altered organization of heterochromatin displays similarities to the structure of heterochromatin in two- to four-cell stage wild-type embryos, suggesting that CAF-1 is required for the maturation of heterochromatin during preimplantation development.
T960	899-901	IN	denotes	In
T961	986-994	VBZ	denotes	displays
T962	902-909	NN	denotes	p150CAF
T963	933-940	NNS	denotes	embryos
T964	909-910	HYPH	denotes	-
T965	910-911	CD	denotes	1
T966	912-918	NN	denotes	mutant
T967	919-921	CD	denotes	16
T968	922-926	NN	denotes	cell
T969	921-922	HYPH	denotes	-
T970	927-932	NN	denotes	stage
T971	940-942	,	denotes	,
T972	942-945	DT	denotes	the
T973	954-966	NN	denotes	organization
T974	946-953	JJ	denotes	altered
T975	967-969	IN	denotes	of
T976	970-985	NN	denotes	heterochromatin
T977	995-1007	NNS	denotes	similarities
T978	1008-1010	IN	denotes	to
T979	1011-1014	DT	denotes	the
T980	1015-1024	NN	denotes	structure
T981	1025-1027	IN	denotes	of
T982	1028-1043	NN	denotes	heterochromatin
T983	1044-1046	IN	denotes	in
T984	1047-1050	CD	denotes	two
T985	1055-1059	CD	denotes	four
T986	1050-1051	HYPH	denotes	-
T987	1052-1054	IN	denotes	to
T988	1060-1064	NN	denotes	cell
T989	1059-1060	HYPH	denotes	-
T990	1065-1070	NN	denotes	stage
T991	1081-1088	NNS	denotes	embryos
T992	1071-1075	JJ	denotes	wild
T993	1076-1080	NN	denotes	type
T994	1075-1076	HYPH	denotes	-
T995	1088-1090	,	denotes	,
T996	1090-1100	VBG	denotes	suggesting
T997	1101-1105	IN	denotes	that
T998	1115-1123	VBN	denotes	required
T999	1106-1109	NN	denotes	CAF
T1000	1109-1110	HYPH	denotes	-
T1001	1110-1111	CD	denotes	1
T1002	1112-1114	VBZ	denotes	is
T1003	1124-1127	IN	denotes	for
T1004	1128-1131	DT	denotes	the
T1005	1132-1142	NN	denotes	maturation
T1006	1143-1145	IN	denotes	of
T1007	1146-1161	NN	denotes	heterochromatin
T1008	1162-1168	IN	denotes	during
T1009	1169-1184	NN	denotes	preimplantation
T1010	1185-1196	NN	denotes	development
T1011	1196-1197	.	denotes	.
T1012	1197-1379	sentence	denotes	In embryonic stem cells, depletion of p150CAF-1 using RNA interference results in the mislocalization, loss of clustering, and decondensation of pericentric heterochromatin domains.
T1013	1198-1200	IN	denotes	In
T1014	1269-1276	VBZ	denotes	results
T1015	1201-1210	JJ	denotes	embryonic
T1016	1216-1221	NNS	denotes	cells
T1017	1211-1215	NN	denotes	stem
T1018	1221-1223	,	denotes	,
T1019	1223-1232	NN	denotes	depletion
T1020	1233-1235	IN	denotes	of
T1021	1236-1243	NN	denotes	p150CAF
T1022	1243-1244	HYPH	denotes	-
T1023	1244-1245	CD	denotes	1
T1024	1246-1251	VBG	denotes	using
T1025	1252-1255	NN	denotes	RNA
T1026	1256-1268	NN	denotes	interference
T1027	1277-1279	IN	denotes	in
T1028	1280-1283	DT	denotes	the
T1029	1284-1299	NN	denotes	mislocalization
T1030	1299-1301	,	denotes	,
T1031	1301-1305	NN	denotes	loss
T1032	1306-1308	IN	denotes	of
T1033	1309-1319	NN	denotes	clustering
T1034	1319-1321	,	denotes	,
T1035	1321-1324	CC	denotes	and
T1036	1325-1339	NN	denotes	decondensation
T1037	1340-1342	IN	denotes	of
T1038	1343-1354	JJ	denotes	pericentric
T1039	1371-1378	NNS	denotes	domains
T1040	1355-1370	NN	denotes	heterochromatin
T1041	1378-1379	.	denotes	.
T1042	1379-1532	sentence	denotes	Furthermore, loss of CAF-1 in these cells results in the alteration of epigenetic histone methylation marks at the level of pericentric heterochromatin.
T1043	1380-1391	RB	denotes	Furthermore
T1044	1422-1429	VBZ	denotes	results
T1045	1391-1393	,	denotes	,
T1046	1393-1397	NN	denotes	loss
T1047	1398-1400	IN	denotes	of
T1048	1401-1404	NN	denotes	CAF
T1049	1404-1405	HYPH	denotes	-
T1050	1405-1406	CD	denotes	1
T1051	1407-1409	IN	denotes	in
T1052	1410-1415	DT	denotes	these
T1053	1416-1421	NNS	denotes	cells
T1054	1430-1432	IN	denotes	in
T1055	1433-1436	DT	denotes	the
T1056	1437-1447	NN	denotes	alteration
T1057	1448-1450	IN	denotes	of
T1058	1451-1461	JJ	denotes	epigenetic
T1059	1470-1481	NN	denotes	methylation
T1060	1462-1469	NN	denotes	histone
T1061	1482-1487	NNS	denotes	marks
T1062	1488-1490	IN	denotes	at
T1063	1491-1494	DT	denotes	the
T1064	1495-1500	NN	denotes	level
T1065	1501-1503	IN	denotes	of
T1066	1504-1515	JJ	denotes	pericentric
T1067	1516-1531	NN	denotes	heterochromatin
T1068	1531-1532	.	denotes	.
T1069	1532-1798	sentence	denotes	These alterations of heterochromatin are not found in p150CAF-1-depleted mouse embryonic fibroblasts, which are cells that are already lineage committed, suggesting that CAF-1 is specifically required for heterochromatin organization in pluripotent embryonic cells.
T1070	1533-1538	DT	denotes	These
T1071	1539-1550	NNS	denotes	alterations
T1072	1578-1583	VBN	denotes	found
T1073	1551-1553	IN	denotes	of
T1074	1554-1569	NN	denotes	heterochromatin
T1075	1570-1573	VBP	denotes	are
T1076	1574-1577	RB	denotes	not
T1077	1584-1586	IN	denotes	in
T1078	1587-1594	NN	denotes	p150CAF
T1079	1597-1605	VBN	denotes	depleted
T1080	1594-1595	HYPH	denotes	-
T1081	1595-1596	CD	denotes	1
T1082	1596-1597	HYPH	denotes	-
T1083	1622-1633	NNS	denotes	fibroblasts
T1084	1606-1611	NN	denotes	mouse
T1085	1612-1621	JJ	denotes	embryonic
T1086	1633-1635	,	denotes	,
T1087	1635-1640	WDT	denotes	which
T1088	1641-1644	VBP	denotes	are
T1089	1645-1650	NNS	denotes	cells
T1090	1651-1655	WDT	denotes	that
T1091	1676-1685	VBN	denotes	committed
T1092	1656-1659	VBP	denotes	are
T1093	1660-1667	RB	denotes	already
T1094	1668-1675	NN	denotes	lineage
T1095	1685-1687	,	denotes	,
T1096	1687-1697	VBG	denotes	suggesting
T1097	1698-1702	IN	denotes	that
T1098	1725-1733	VBN	denotes	required
T1099	1703-1706	NN	denotes	CAF
T1100	1706-1707	HYPH	denotes	-
T1101	1707-1708	CD	denotes	1
T1102	1709-1711	VBZ	denotes	is
T1103	1712-1724	RB	denotes	specifically
T1104	1734-1737	IN	denotes	for
T1105	1738-1753	NN	denotes	heterochromatin
T1106	1754-1766	NN	denotes	organization
T1107	1767-1769	IN	denotes	in
T1108	1770-1781	JJ	denotes	pluripotent
T1109	1792-1797	NNS	denotes	cells
T1110	1782-1791	JJ	denotes	embryonic
T1111	1797-1798	.	denotes	.
T1112	1798-1986	sentence	denotes	Our findings underline the role of the chromatin assembly machinery in controlling the spatial organization and epigenetic marking of the genome in early embryos and embryonic stem cells.
T1113	1799-1802	PRP$	denotes	Our
T1114	1803-1811	NNS	denotes	findings
T1115	1812-1821	VBP	denotes	underline
T1116	1822-1825	DT	denotes	the
T1117	1826-1830	NN	denotes	role
T1118	1831-1833	IN	denotes	of
T1119	1834-1837	DT	denotes	the
T1120	1857-1866	NN	denotes	machinery
T1121	1838-1847	NN	denotes	chromatin
T1122	1848-1856	NN	denotes	assembly
T1123	1867-1869	IN	denotes	in
T1124	1870-1881	VBG	denotes	controlling
T1125	1882-1885	DT	denotes	the
T1126	1894-1906	NN	denotes	organization
T1127	1886-1893	JJ	denotes	spatial
T1128	1907-1910	CC	denotes	and
T1129	1911-1921	JJ	denotes	epigenetic
T1130	1922-1929	NN	denotes	marking
T1131	1930-1932	IN	denotes	of
T1132	1933-1936	DT	denotes	the
T1133	1937-1943	NN	denotes	genome
T1134	1944-1946	IN	denotes	in
T1135	1947-1952	JJ	denotes	early
T1136	1953-1960	NNS	denotes	embryos
T1137	1961-1964	CC	denotes	and
T1138	1965-1974	JJ	denotes	embryonic
T1139	1980-1985	NNS	denotes	cells
T1140	1975-1979	NN	denotes	stem
T1141	1985-1986	.	denotes	.
T2297	3237-3243	IN	denotes	During
T2298	3291-3300	VBZ	denotes	undergoes
T2299	3244-3249	NN	denotes	mouse
T2300	3267-3278	NN	denotes	development
T2301	3250-3266	NN	denotes	pre-implantation
T2302	3278-3280	,	denotes	,
T2303	3280-3283	DT	denotes	the
T2304	3284-3290	NN	denotes	genome
T2305	3301-3302	DT	denotes	a
T2306	3303-3309	NN	denotes	series
T2307	3310-3312	IN	denotes	of
T2308	3313-3318	JJ	denotes	major
T2309	3330-3337	NNS	denotes	changes
T2310	3319-3329	JJ	denotes	epigenetic
T2311	3338-3346	VBN	denotes	required
T2312	3347-3350	IN	denotes	for
T2313	3351-3360	JJ	denotes	embryonic
T2314	3366-3376	NN	denotes	expression
T2315	3361-3365	NN	denotes	gene
T2316	3376-3378	,	denotes	,
T2317	3378-3381	DT	denotes	the
T2318	3382-3393	NN	denotes	maintenance
T2319	3394-3396	IN	denotes	of
T2320	3397-3408	NN	denotes	totipotency
T2321	3408-3410	,	denotes	,
T2322	3410-3413	CC	denotes	and
T2323	3414-3417	DT	denotes	the
T2324	3440-3446	NNS	denotes	events
T2325	3418-3423	JJ	denotes	first
T2326	3424-3439	NN	denotes	differentiation
T2327	3447-3448	-LRB-	denotes	[
T2328	3450-3451	CD	denotes	2
T2329	3448-3449	CD	denotes	1
T2330	3449-3450	,	denotes	,
T2331	3451-3452	-RRB-	denotes	]
T2332	3452-3453	.	denotes	.
T2333	3453-3624	sentence	denotes	While many studies have established the importance of DNA methylation in epigenetic reprogramming, recent data point to a crucial role of chromatin in this process [3–5].
T2334	3454-3459	IN	denotes	While
T2335	3478-3489	VBN	denotes	established
T2336	3460-3464	JJ	denotes	many
T2337	3465-3472	NNS	denotes	studies
T2338	3473-3477	VBP	denotes	have
T2339	3565-3570	VBP	denotes	point
T2340	3490-3493	DT	denotes	the
T2341	3494-3504	NN	denotes	importance
T2342	3505-3507	IN	denotes	of
T2343	3508-3511	NN	denotes	DNA
T2344	3512-3523	NN	denotes	methylation
T2345	3524-3526	IN	denotes	in
T2346	3527-3537	JJ	denotes	epigenetic
T2347	3538-3551	NN	denotes	reprogramming
T2348	3551-3553	,	denotes	,
T2349	3553-3559	JJ	denotes	recent
T2350	3560-3564	NNS	denotes	data
T2351	3571-3573	IN	denotes	to
T2352	3574-3575	DT	denotes	a
T2353	3584-3588	NN	denotes	role
T2354	3576-3583	JJ	denotes	crucial
T2355	3589-3591	IN	denotes	of
T2356	3592-3601	NN	denotes	chromatin
T2357	3602-3604	IN	denotes	in
T2358	3605-3609	DT	denotes	this
T2359	3610-3617	NN	denotes	process
T2360	3618-3619	-LRB-	denotes	[
T2361	3619-3620	CD	denotes	3
T2362	3620-3621	SYM	denotes	–
T2363	3621-3622	CD	denotes	5
T2364	3622-3623	-RRB-	denotes	]
T2365	3623-3624	.	denotes	.
T2366	3624-3832	sentence	denotes	Yet we know very little about the role of histone modifying enzymes, chromatin remodeling factors, and histone chaperones during pre-implantation development, or in stem cells derived from early embryos [6].
T2367	3625-3628	RB	denotes	Yet
T2368	3632-3636	VBP	denotes	know
T2369	3629-3631	PRP	denotes	we
T2370	3637-3641	RB	denotes	very
T2371	3642-3648	JJ	denotes	little
T2372	3649-3654	IN	denotes	about
T2373	3655-3658	DT	denotes	the
T2374	3659-3663	NN	denotes	role
T2375	3664-3666	IN	denotes	of
T2376	3667-3674	NN	denotes	histone
T2377	3675-3684	VBG	denotes	modifying
T2378	3685-3692	NNS	denotes	enzymes
T2379	3692-3694	,	denotes	,
T2380	3694-3703	NN	denotes	chromatin
T2381	3704-3714	NN	denotes	remodeling
T2382	3715-3722	NNS	denotes	factors
T2383	3722-3724	,	denotes	,
T2384	3724-3727	CC	denotes	and
T2385	3728-3735	NN	denotes	histone
T2386	3736-3746	NNS	denotes	chaperones
T2387	3747-3753	IN	denotes	during
T2388	3754-3770	NN	denotes	pre-implantation
T2389	3771-3782	NN	denotes	development
T2390	3782-3784	,	denotes	,
T2391	3784-3786	CC	denotes	or
T2392	3787-3789	IN	denotes	in
T2393	3790-3794	NN	denotes	stem
T2394	3795-3800	NNS	denotes	cells
T2395	3801-3808	VBN	denotes	derived
T2396	3809-3813	IN	denotes	from
T2397	3814-3819	JJ	denotes	early
T2398	3820-3827	NNS	denotes	embryos
T2399	3828-3829	-LRB-	denotes	[
T2400	3829-3830	CD	denotes	6
T2401	3830-3831	-RRB-	denotes	]
T2402	3831-3832	.	denotes	.
T2403	3832-4078	sentence	denotes	A subset of identified histone chaperones and chromatin remodeling complexes can collaborate to promote nucleosome assembly in vitro and are therefore in a strategic position to control chromatin assembly and maturation during development [7,8].
T2404	3833-3834	DT	denotes	A
T2405	3835-3841	NN	denotes	subset
T2406	3914-3925	VB	denotes	collaborate
T2407	3842-3844	IN	denotes	of
T2408	3845-3855	VBN	denotes	identified
T2409	3864-3874	NNS	denotes	chaperones
T2410	3856-3863	NN	denotes	histone
T2411	3875-3878	CC	denotes	and
T2412	3879-3888	NN	denotes	chromatin
T2413	3889-3899	NN	denotes	remodeling
T2414	3900-3909	NNS	denotes	complexes
T2415	3910-3913	MD	denotes	can
T2416	3926-3928	TO	denotes	to
T2417	3929-3936	VB	denotes	promote
T2418	3937-3947	NN	denotes	nucleosome
T2419	3948-3956	NN	denotes	assembly
T2420	3957-3959	FW	denotes	in
T2421	3960-3965	FW	denotes	vitro
T2422	3966-3969	CC	denotes	and
T2423	3970-3973	VBP	denotes	are
T2424	3974-3983	RB	denotes	therefore
T2425	3984-3986	IN	denotes	in
T2426	3987-3988	DT	denotes	a
T2427	3999-4007	NN	denotes	position
T2428	3989-3998	JJ	denotes	strategic
T2429	4008-4010	TO	denotes	to
T2430	4011-4018	VB	denotes	control
T2431	4019-4028	NN	denotes	chromatin
T2432	4029-4037	NN	denotes	assembly
T2433	4038-4041	CC	denotes	and
T2434	4042-4052	NN	denotes	maturation
T2435	4053-4059	IN	denotes	during
T2436	4060-4071	NN	denotes	development
T2437	4072-4073	-LRB-	denotes	[
T2438	4075-4076	CD	denotes	8
T2439	4073-4074	CD	denotes	7
T2440	4074-4075	,	denotes	,
T2441	4076-4077	-RRB-	denotes	]
T2442	4077-4078	.	denotes	.
T2443	4078-4300	sentence	denotes	Among histone chaperones, chromatin assembly factor 1 (CAF-1) is a three-subunit (p150, p60, and p48) complex which promotes histone H3 and H4 deposition onto newly synthesized DNA during replication or DNA repair [9,10].
T2444	4079-4084	IN	denotes	Among
T2445	4141-4143	VBZ	denotes	is
T2446	4085-4092	NN	denotes	histone
T2447	4093-4103	NNS	denotes	chaperones
T2448	4103-4105	,	denotes	,
T2449	4105-4114	NN	denotes	chromatin
T2450	4115-4123	NN	denotes	assembly
T2451	4124-4130	NN	denotes	factor
T2452	4131-4132	CD	denotes	1
T2453	4133-4134	-LRB-	denotes	(
T2454	4134-4137	NN	denotes	CAF
T2455	4137-4138	HYPH	denotes	-
T2456	4138-4139	CD	denotes	1
T2457	4139-4140	-RRB-	denotes	)
T2458	4144-4145	DT	denotes	a
T2459	4181-4188	NN	denotes	complex
T2460	4146-4151	CD	denotes	three
T2461	4152-4159	NN	denotes	subunit
T2462	4151-4152	HYPH	denotes	-
T2463	4160-4161	-LRB-	denotes	(
T2464	4161-4165	NN	denotes	p150
T2465	4165-4167	,	denotes	,
T2466	4167-4170	NN	denotes	p60
T2467	4170-4172	,	denotes	,
T2468	4172-4175	CC	denotes	and
T2469	4176-4179	NN	denotes	p48
T2470	4179-4180	-RRB-	denotes	)
T2471	4189-4194	WDT	denotes	which
T2472	4195-4203	VBZ	denotes	promotes
T2473	4204-4211	NN	denotes	histone
T2474	4212-4214	NN	denotes	H3
T2475	4222-4232	NN	denotes	deposition
T2476	4215-4218	CC	denotes	and
T2477	4219-4221	NN	denotes	H4
T2478	4233-4237	IN	denotes	onto
T2479	4238-4243	RB	denotes	newly
T2480	4244-4255	VBN	denotes	synthesized
T2481	4256-4259	NN	denotes	DNA
T2482	4260-4266	IN	denotes	during
T2483	4267-4278	NN	denotes	replication
T2484	4279-4281	CC	denotes	or
T2485	4282-4285	NN	denotes	DNA
T2486	4286-4292	NN	denotes	repair
T2487	4293-4294	-LRB-	denotes	[
T2488	4296-4298	CD	denotes	10
T2489	4294-4295	CD	denotes	9
T2490	4295-4296	,	denotes	,
T2491	4298-4299	-RRB-	denotes	]
T2492	4299-4300	.	denotes	.
T2493	4300-4552	sentence	denotes	Specifically, CAF-1 deposits the histone H3 variant H3.1 into chromatin, in a pathway coupled to DNA synthesis, whereas a second histone chaperone, HIRA, is involved in the deposition of the H3.3 variant in a DNA synthesis independent pathway [11,12].
T2494	4301-4313	RB	denotes	Specifically
T2495	4321-4329	VBZ	denotes	deposits
T2496	4313-4315	,	denotes	,
T2497	4315-4318	NN	denotes	CAF
T2498	4318-4319	HYPH	denotes	-
T2499	4319-4320	CD	denotes	1
T2500	4330-4333	DT	denotes	the
T2501	4345-4352	NN	denotes	variant
T2502	4334-4341	NN	denotes	histone
T2503	4342-4344	NN	denotes	H3
T2504	4353-4357	NN	denotes	H3.1
T2505	4358-4362	IN	denotes	into
T2506	4363-4372	NN	denotes	chromatin
T2507	4372-4374	,	denotes	,
T2508	4374-4376	IN	denotes	in
T2509	4377-4378	DT	denotes	a
T2510	4379-4386	NN	denotes	pathway
T2511	4387-4394	VBN	denotes	coupled
T2512	4395-4397	IN	denotes	to
T2513	4398-4401	NN	denotes	DNA
T2514	4402-4411	NN	denotes	synthesis
T2515	4411-4413	,	denotes	,
T2516	4413-4420	IN	denotes	whereas
T2517	4458-4466	VBN	denotes	involved
T2518	4421-4422	DT	denotes	a
T2519	4438-4447	NN	denotes	chaperone
T2520	4423-4429	JJ	denotes	second
T2521	4430-4437	NN	denotes	histone
T2522	4447-4449	,	denotes	,
T2523	4449-4453	NN	denotes	HIRA
T2524	4453-4455	,	denotes	,
T2525	4455-4457	VBZ	denotes	is
T2526	4467-4469	IN	denotes	in
T2527	4470-4473	DT	denotes	the
T2528	4474-4484	NN	denotes	deposition
T2529	4485-4487	IN	denotes	of
T2530	4488-4491	DT	denotes	the
T2531	4497-4504	NN	denotes	variant
T2532	4492-4496	NN	denotes	H3.3
T2533	4505-4507	IN	denotes	in
T2534	4508-4509	DT	denotes	a
T2535	4536-4543	NN	denotes	pathway
T2536	4510-4513	NN	denotes	DNA
T2537	4514-4523	NN	denotes	synthesis
T2538	4524-4535	JJ	denotes	independent
T2539	4544-4545	-LRB-	denotes	[
T2540	4548-4550	CD	denotes	12
T2541	4545-4547	CD	denotes	11
T2542	4547-4548	,	denotes	,
T2543	4550-4551	-RRB-	denotes	]
T2544	4551-4552	.	denotes	.
T2545	4552-4859	sentence	denotes	In addition to this specific chromatin assembly activity, CAF-1 interacts with several proteins present in heterochromatin, including heterochromatin protein 1 (HP1) and MBD1, a methyl-CpG binding domain protein that recruits histone deacetylase and repressive histone methyltransferase activities [13–15].
T2546	4553-4555	IN	denotes	In
T2547	4617-4626	VBZ	denotes	interacts
T2548	4556-4564	NN	denotes	addition
T2549	4565-4567	IN	denotes	to
T2550	4568-4572	DT	denotes	this
T2551	4601-4609	NN	denotes	activity
T2552	4573-4581	JJ	denotes	specific
T2553	4582-4591	NN	denotes	chromatin
T2554	4592-4600	NN	denotes	assembly
T2555	4609-4611	,	denotes	,
T2556	4611-4614	NN	denotes	CAF
T2557	4614-4615	HYPH	denotes	-
T2558	4615-4616	CD	denotes	1
T2559	4627-4631	IN	denotes	with
T2560	4632-4639	JJ	denotes	several
T2561	4640-4648	NN	denotes	proteins
T2562	4649-4656	JJ	denotes	present
T2563	4657-4659	IN	denotes	in
T2564	4660-4675	NN	denotes	heterochromatin
T2565	4675-4677	,	denotes	,
T2566	4677-4686	VBG	denotes	including
T2567	4687-4702	NN	denotes	heterochromatin
T2568	4703-4710	NN	denotes	protein
T2569	4711-4712	CD	denotes	1
T2570	4713-4714	-LRB-	denotes	(
T2571	4714-4717	NN	denotes	HP1
T2572	4717-4718	-RRB-	denotes	)
T2573	4719-4722	CC	denotes	and
T2574	4723-4727	NN	denotes	MBD1
T2575	4727-4729	,	denotes	,
T2576	4729-4730	DT	denotes	a
T2577	4757-4764	NN	denotes	protein
T2578	4731-4737	NN	denotes	methyl
T2579	4738-4741	NN	denotes	CpG
T2580	4737-4738	HYPH	denotes	-
T2581	4742-4749	NN	denotes	binding
T2582	4750-4756	NN	denotes	domain
T2583	4765-4769	WDT	denotes	that
T2584	4770-4778	VBZ	denotes	recruits
T2585	4779-4786	NN	denotes	histone
T2586	4787-4798	NN	denotes	deacetylase
T2587	4840-4850	NNS	denotes	activities
T2588	4799-4802	CC	denotes	and
T2589	4803-4813	JJ	denotes	repressive
T2590	4822-4839	NN	denotes	methyltransferase
T2591	4814-4821	NN	denotes	histone
T2592	4851-4852	-LRB-	denotes	[
T2593	4852-4854	CD	denotes	13
T2594	4854-4855	SYM	denotes	–
T2595	4855-4857	CD	denotes	15
T2596	4857-4858	-RRB-	denotes	]
T2597	4858-4859	.	denotes	.
T2598	4859-5031	sentence	denotes	Furthermore, p150CAF-1 is required to ensure a replication-specific pool of HP1 molecules at replication sites in pericentric heterochromatin during mid-late S phase [16].
T2599	4860-4871	RB	denotes	Furthermore
T2600	4886-4894	VBN	denotes	required
T2601	4871-4873	,	denotes	,
T2602	4873-4880	NN	denotes	p150CAF
T2603	4880-4881	HYPH	denotes	-
T2604	4881-4882	CD	denotes	1
T2605	4883-4885	VBZ	denotes	is
T2606	4895-4897	TO	denotes	to
T2607	4898-4904	VB	denotes	ensure
T2608	4905-4906	DT	denotes	a
T2609	4928-4932	NN	denotes	pool
T2610	4907-4918	NN	denotes	replication
T2611	4919-4927	JJ	denotes	specific
T2612	4918-4919	HYPH	denotes	-
T2613	4933-4935	IN	denotes	of
T2614	4936-4939	NN	denotes	HP1
T2615	4940-4949	NNS	denotes	molecules
T2616	4950-4952	IN	denotes	at
T2617	4953-4964	NN	denotes	replication
T2618	4965-4970	NNS	denotes	sites
T2619	4971-4973	IN	denotes	in
T2620	4974-4985	JJ	denotes	pericentric
T2621	4986-5001	NN	denotes	heterochromatin
T2622	5002-5008	IN	denotes	during
T2623	5009-5012	JJ	denotes	mid
T2624	5013-5017	JJ	denotes	late
T2625	5012-5013	SYM	denotes	-
T2626	5020-5025	NN	denotes	phase
T2627	5018-5019	NN	denotes	S
T2628	5026-5027	-LRB-	denotes	[
T2629	5027-5029	CD	denotes	16
T2630	5029-5030	-RRB-	denotes	]
T2631	5030-5031	.	denotes	.
T2632	5031-5164	sentence	denotes	Taken together, these data suggest roles for CAF-1 in the formation of heterochromatin and in the heritability of epigenetic traits.
T2633	5032-5037	VBN	denotes	Taken
T2634	5059-5066	VBP	denotes	suggest
T2635	5038-5046	RB	denotes	together
T2636	5046-5048	,	denotes	,
T2637	5048-5053	DT	denotes	these
T2638	5054-5058	NNS	denotes	data
T2639	5067-5072	NNS	denotes	roles
T2640	5073-5076	IN	denotes	for
T2641	5077-5080	NN	denotes	CAF
T2642	5080-5081	HYPH	denotes	-
T2643	5081-5082	CD	denotes	1
T2644	5083-5085	IN	denotes	in
T2645	5086-5089	DT	denotes	the
T2646	5090-5099	NN	denotes	formation
T2647	5100-5102	IN	denotes	of
T2648	5103-5118	NN	denotes	heterochromatin
T2649	5119-5122	CC	denotes	and
T2650	5123-5125	IN	denotes	in
T2651	5126-5129	DT	denotes	the
T2652	5130-5142	NN	denotes	heritability
T2653	5143-5145	IN	denotes	of
T2654	5146-5156	JJ	denotes	epigenetic
T2655	5157-5163	NNS	denotes	traits
T2656	5163-5164	.	denotes	.
T2657	5164-5424	sentence	denotes	While the function of this evolutionary conserved histone chaperone has been studied extensively biochemically, we still lack information concerning its importance during early development in mammals and in pluripotent cells such as embryonic stem (ES) cells.
T2658	5165-5170	IN	denotes	While
T2659	5242-5249	VBN	denotes	studied
T2660	5171-5174	DT	denotes	the
T2661	5175-5183	NN	denotes	function
T2662	5184-5186	IN	denotes	of
T2663	5187-5191	DT	denotes	this
T2664	5223-5232	NN	denotes	chaperone
T2665	5192-5204	JJ	denotes	evolutionary
T2666	5205-5214	VBN	denotes	conserved
T2667	5215-5222	NN	denotes	histone
T2668	5233-5236	VBZ	denotes	has
T2669	5237-5241	VBN	denotes	been
T2670	5286-5290	VBP	denotes	lack
T2671	5250-5261	RB	denotes	extensively
T2672	5262-5275	RB	denotes	biochemically
T2673	5275-5277	,	denotes	,
T2674	5277-5279	PRP	denotes	we
T2675	5280-5285	RB	denotes	still
T2676	5291-5302	NN	denotes	information
T2677	5303-5313	VBG	denotes	concerning
T2678	5314-5317	PRP$	denotes	its
T2679	5318-5328	NN	denotes	importance
T2680	5329-5335	IN	denotes	during
T2681	5336-5341	JJ	denotes	early
T2682	5342-5353	NN	denotes	development
T2683	5354-5356	IN	denotes	in
T2684	5357-5364	NNS	denotes	mammals
T2685	5365-5368	CC	denotes	and
T2686	5369-5371	IN	denotes	in
T2687	5372-5383	JJ	denotes	pluripotent
T2688	5384-5389	NNS	denotes	cells
T2689	5390-5394	JJ	denotes	such
T2690	5395-5397	IN	denotes	as
T2691	5398-5407	JJ	denotes	embryonic
T2692	5408-5412	NN	denotes	stem
T2693	5418-5423	NNS	denotes	cells
T2694	5413-5414	-LRB-	denotes	(
T2695	5414-5416	NN	denotes	ES
T2696	5416-5417	-RRB-	denotes	)
T2697	5423-5424	.	denotes	.
T2698	5424-5582	sentence	denotes	Here, we have analyzed the importance of CAF-1 during early mouse development by genetic ablation and in ES cells by depletion using RNA interference (RNAi).
T2699	5425-5429	RB	denotes	Here
T2700	5439-5447	VBN	denotes	analyzed
T2701	5429-5431	,	denotes	,
T2702	5431-5433	PRP	denotes	we
T2703	5434-5438	VBP	denotes	have
T2704	5448-5451	DT	denotes	the
T2705	5452-5462	NN	denotes	importance
T2706	5463-5465	IN	denotes	of
T2707	5466-5469	NN	denotes	CAF
T2708	5469-5470	HYPH	denotes	-
T2709	5470-5471	CD	denotes	1
T2710	5472-5478	IN	denotes	during
T2711	5479-5484	JJ	denotes	early
T2712	5491-5502	NN	denotes	development
T2713	5485-5490	NN	denotes	mouse
T2714	5503-5505	IN	denotes	by
T2715	5506-5513	JJ	denotes	genetic
T2716	5514-5522	NN	denotes	ablation
T2717	5523-5526	CC	denotes	and
T2718	5527-5529	IN	denotes	in
T2719	5530-5532	NN	denotes	ES
T2720	5533-5538	NNS	denotes	cells
T2721	5539-5541	IN	denotes	by
T2722	5542-5551	NN	denotes	depletion
T2723	5552-5557	VBG	denotes	using
T2724	5558-5561	NN	denotes	RNA
T2725	5562-5574	NN	denotes	interference
T2726	5575-5576	-LRB-	denotes	(
T2727	5576-5580	NN	denotes	RNAi
T2728	5580-5581	-RRB-	denotes	)
T2729	5581-5582	.	denotes	.
T2730	5582-5665	sentence	denotes	We show that CAF-1 is essential for viability in early mouse embryos and ES cells.
T2731	5583-5585	PRP	denotes	We
T2732	5586-5590	VBP	denotes	show
T2733	5591-5595	IN	denotes	that
T2734	5602-5604	VBZ	denotes	is
T2735	5596-5599	NN	denotes	CAF
T2736	5599-5600	HYPH	denotes	-
T2737	5600-5601	CD	denotes	1
T2738	5605-5614	JJ	denotes	essential
T2739	5615-5618	IN	denotes	for
T2740	5619-5628	NN	denotes	viability
T2741	5629-5631	IN	denotes	in
T2742	5632-5637	JJ	denotes	early
T2743	5644-5651	NNS	denotes	embryos
T2744	5638-5643	NN	denotes	mouse
T2745	5652-5655	CC	denotes	and
T2746	5656-5658	NN	denotes	ES
T2747	5659-5664	NNS	denotes	cells
T2748	5664-5665	.	denotes	.
T2749	5665-5819	sentence	denotes	We provide evidence that CAF-1 is required for the spatial organization and epigenetic marking of heterochromatin domains in pluripotent embryonic cells.
T2750	5666-5668	PRP	denotes	We
T2751	5669-5676	VBP	denotes	provide
T2752	5677-5685	NN	denotes	evidence
T2753	5686-5690	IN	denotes	that
T2754	5700-5708	VBN	denotes	required
T2755	5691-5694	NN	denotes	CAF
T2756	5694-5695	HYPH	denotes	-
T2757	5695-5696	CD	denotes	1
T2758	5697-5699	VBZ	denotes	is
T2759	5709-5712	IN	denotes	for
T2760	5713-5716	DT	denotes	the
T2761	5725-5737	NN	denotes	organization
T2762	5717-5724	JJ	denotes	spatial
T2763	5738-5741	CC	denotes	and
T2764	5742-5752	JJ	denotes	epigenetic
T2765	5753-5760	NN	denotes	marking
T2766	5761-5763	IN	denotes	of
T2767	5764-5779	NN	denotes	heterochromatin
T2768	5780-5787	NNS	denotes	domains
T2769	5788-5790	IN	denotes	in
T2770	5791-5802	JJ	denotes	pluripotent
T2771	5813-5818	NNS	denotes	cells
T2772	5803-5812	JJ	denotes	embryonic
T2773	5818-5819	.	denotes	.
T7971	5829-5831	PRP	denotes	We
T7972	5832-5836	VBD	denotes	used
T7973	5837-5841	NN	denotes	gene
T7974	5842-5851	NN	denotes	targeting
T7975	5852-5854	IN	denotes	in
T7976	5855-5857	NN	denotes	ES
T7977	5858-5863	NNS	denotes	cells
T7978	5864-5866	TO	denotes	to
T7979	5867-5873	VB	denotes	delete
T7980	5874-5878	NN	denotes	exon
T7981	5879-5880	CD	denotes	3
T7982	5881-5883	IN	denotes	in
T7983	5884-5887	DT	denotes	the
T7984	5895-5899	NN	denotes	gene
T7985	5888-5894	NN	denotes	Chaf1a
T7986	5899-5901	,	denotes	,
T7987	5901-5906	WDT	denotes	which
T7988	5907-5914	VBZ	denotes	encodes
T7989	5915-5922	NN	denotes	p150CAF
T7990	5922-5923	HYPH	denotes	-
T7991	5923-5924	CD	denotes	1
T7992	5925-5926	-LRB-	denotes	(
T7993	5933-5935	NN	denotes	1A
T7994	5926-5932	NN	denotes	Figure
T7995	5935-5936	-RRB-	denotes	)
T7996	5936-5937	.	denotes	.
T7997	5937-6075	sentence	denotes	Chaf1a +/− mice were born at a Mendelian frequency, were of normal size and weight, displayed no obvious abnormalities, and were fertile.
T7998	5938-5944	NN	denotes	Chaf1a
T7999	5949-5953	NNS	denotes	mice
T8000	5945-5946	SYM	denotes	+
T8001	5946-5947	HYPH	denotes	/
T8002	5947-5948	SYM	denotes	−
T8003	5959-5963	VBN	denotes	born
T8004	5954-5958	VBD	denotes	were
T8005	5964-5966	IN	denotes	at
T8006	5967-5968	DT	denotes	a
T8007	5979-5988	NN	denotes	frequency
T8008	5969-5978	JJ	denotes	Mendelian
T8009	5988-5990	,	denotes	,
T8010	5990-5994	VBD	denotes	were
T8011	5995-5997	IN	denotes	of
T8012	5998-6004	JJ	denotes	normal
T8013	6005-6009	NN	denotes	size
T8014	6010-6013	CC	denotes	and
T8015	6014-6020	NN	denotes	weight
T8016	6020-6022	,	denotes	,
T8017	6022-6031	VBD	denotes	displayed
T8018	6032-6034	DT	denotes	no
T8019	6043-6056	NNS	denotes	abnormalities
T8020	6035-6042	JJ	denotes	obvious
T8021	6056-6058	,	denotes	,
T8022	6058-6061	CC	denotes	and
T8023	6062-6066	VBD	denotes	were
T8024	6067-6074	JJ	denotes	fertile
T8025	6074-6075	.	denotes	.
T8026	6075-6153	sentence	denotes	Crossing heterozygous mice failed to generate viable newborn Chaf1a −/− mice.
T8027	6076-6084	VBG	denotes	Crossing
T8028	6103-6109	VBD	denotes	failed
T8029	6085-6097	JJ	denotes	heterozygous
T8030	6098-6102	NNS	denotes	mice
T8031	6110-6112	TO	denotes	to
T8032	6113-6121	VB	denotes	generate
T8033	6122-6128	JJ	denotes	viable
T8034	6148-6152	NNS	denotes	mice
T8035	6129-6136	JJ	denotes	newborn
T8036	6137-6143	NN	denotes	Chaf1a
T8037	6144-6145	SYM	denotes	−
T8038	6145-6146	HYPH	denotes	/
T8039	6146-6147	SYM	denotes	−
T8040	6152-6153	.	denotes	.
T8041	6153-6253	sentence	denotes	Furthermore, no homozygous Chaf1a −/− embryos were detected at any of the post-implantation stages.
T8042	6154-6165	RB	denotes	Furthermore
T8043	6205-6213	VBN	denotes	detected
T8044	6165-6167	,	denotes	,
T8045	6167-6169	DT	denotes	no
T8046	6192-6199	NNS	denotes	embryos
T8047	6170-6180	JJ	denotes	homozygous
T8048	6181-6187	NN	denotes	Chaf1a
T8049	6188-6189	SYM	denotes	−
T8050	6189-6190	HYPH	denotes	/
T8051	6190-6191	SYM	denotes	−
T8052	6200-6204	VBD	denotes	were
T8053	6214-6216	IN	denotes	at
T8054	6217-6220	DT	denotes	any
T8055	6221-6223	IN	denotes	of
T8056	6224-6227	DT	denotes	the
T8057	6246-6252	NNS	denotes	stages
T8058	6228-6245	JJ	denotes	post-implantation
T8059	6252-6253	.	denotes	.
T8060	6253-6355	sentence	denotes	However, we could detect Chaf1a −/− embryos at embryonic day 4 (E4) using a PCR strategy (Figure 1B).
T8061	6254-6261	RB	denotes	However
T8062	6272-6278	VB	denotes	detect
T8063	6261-6263	,	denotes	,
T8064	6263-6265	PRP	denotes	we
T8065	6266-6271	MD	denotes	could
T8066	6279-6285	NN	denotes	Chaf1a
T8067	6290-6297	NNS	denotes	embryos
T8068	6286-6287	SYM	denotes	−
T8069	6287-6288	HYPH	denotes	/
T8070	6288-6289	SYM	denotes	−
T8071	6298-6300	IN	denotes	at
T8072	6301-6310	JJ	denotes	embryonic
T8073	6311-6314	NN	denotes	day
T8074	6315-6316	CD	denotes	4
T8075	6317-6318	-LRB-	denotes	(
T8076	6318-6320	NN	denotes	E4
T8077	6320-6321	-RRB-	denotes	)
T8078	6322-6327	VBG	denotes	using
T8079	6328-6329	DT	denotes	a
T8080	6334-6342	NN	denotes	strategy
T8081	6330-6333	NN	denotes	PCR
T8082	6343-6344	-LRB-	denotes	(
T8083	6351-6353	NN	denotes	1B
T8084	6344-6350	NN	denotes	Figure
T8085	6353-6354	-RRB-	denotes	)
T8086	6354-6355	.	denotes	.
T8087	6355-6536	sentence	denotes	They represent only 10% of E4 embryos obtained from Chaf1a +/− mice intercrosses, suggesting that more than half of the homozygous mutant embryos had degenerated before this stage.
T8088	6356-6360	PRP	denotes	They
T8089	6361-6370	VBP	denotes	represent
T8090	6371-6375	RB	denotes	only
T8091	6376-6378	CD	denotes	10
T8092	6378-6379	NN	denotes	%
T8093	6380-6382	IN	denotes	of
T8094	6383-6385	NN	denotes	E4
T8095	6386-6393	NNS	denotes	embryos
T8096	6394-6402	VBN	denotes	obtained
T8097	6403-6407	IN	denotes	from
T8098	6408-6414	NN	denotes	Chaf1a
T8099	6424-6436	NNS	denotes	intercrosses
T8100	6415-6416	SYM	denotes	+
T8101	6416-6417	HYPH	denotes	/
T8102	6417-6418	SYM	denotes	−
T8103	6419-6423	NNS	denotes	mice
T8104	6436-6438	,	denotes	,
T8105	6438-6448	VBG	denotes	suggesting
T8106	6449-6453	IN	denotes	that
T8107	6506-6517	VBN	denotes	degenerated
T8108	6454-6458	JJR	denotes	more
T8109	6464-6468	NN	denotes	half
T8110	6459-6463	IN	denotes	than
T8111	6469-6471	IN	denotes	of
T8112	6472-6475	DT	denotes	the
T8113	6494-6501	NNS	denotes	embryos
T8114	6476-6486	JJ	denotes	homozygous
T8115	6487-6493	NN	denotes	mutant
T8116	6502-6505	VBD	denotes	had
T8117	6518-6524	IN	denotes	before
T8118	6525-6529	DT	denotes	this
T8119	6530-6535	NN	denotes	stage
T8120	6535-6536	.	denotes	.
T8121	6536-6702	sentence	denotes	Moreover, mutant embryos contained only eight to 16 cells at the E4 stage, instead of 32 cells observed for wild-type or heterozygous blastocysts (Figure 1C and 1D).
T8122	6537-6545	RB	denotes	Moreover
T8123	6562-6571	VBD	denotes	contained
T8124	6545-6547	,	denotes	,
T8125	6547-6553	NN	denotes	mutant
T8126	6554-6561	NNS	denotes	embryos
T8127	6572-6576	RB	denotes	only
T8128	6586-6588	CD	denotes	16
T8129	6577-6582	CD	denotes	eight
T8130	6583-6585	IN	denotes	to
T8131	6589-6594	NNS	denotes	cells
T8132	6595-6597	IN	denotes	at
T8133	6598-6601	DT	denotes	the
T8134	6605-6610	NN	denotes	stage
T8135	6602-6604	NN	denotes	E4
T8136	6610-6612	,	denotes	,
T8137	6612-6619	RB	denotes	instead
T8138	6620-6622	IN	denotes	of
T8139	6623-6625	CD	denotes	32
T8140	6626-6631	NNS	denotes	cells
T8141	6632-6640	VBN	denotes	observed
T8142	6641-6644	IN	denotes	for
T8143	6645-6649	JJ	denotes	wild
T8144	6650-6654	NN	denotes	type
T8145	6649-6650	HYPH	denotes	-
T8146	6671-6682	NNS	denotes	blastocysts
T8147	6655-6657	CC	denotes	or
T8148	6658-6670	JJ	denotes	heterozygous
T8149	6683-6684	-LRB-	denotes	(
T8150	6691-6693	NN	denotes	1C
T8151	6684-6690	NN	denotes	Figure
T8152	6694-6697	CC	denotes	and
T8153	6698-6700	NN	denotes	1D
T8154	6700-6701	-RRB-	denotes	)
T8155	6701-6702	.	denotes	.
T8156	6702-6833	sentence	denotes	Further analysis by light microscopy using immunofluorescence (IF) allowed us to compare wild-type and p150CAF-1-depleted embryos.
T8157	6703-6710	JJ	denotes	Further
T8158	6711-6719	NN	denotes	analysis
T8159	6770-6777	VBD	denotes	allowed
T8160	6720-6722	IN	denotes	by
T8161	6723-6728	NN	denotes	light
T8162	6729-6739	NN	denotes	microscopy
T8163	6740-6745	VBG	denotes	using
T8164	6746-6764	NN	denotes	immunofluorescence
T8165	6765-6766	-LRB-	denotes	(
T8166	6766-6768	NN	denotes	IF
T8167	6768-6769	-RRB-	denotes	)
T8168	6778-6780	PRP	denotes	us
T8169	6784-6791	VB	denotes	compare
T8170	6781-6783	TO	denotes	to
T8171	6792-6796	JJ	denotes	wild
T8172	6797-6801	NN	denotes	type
T8173	6796-6797	HYPH	denotes	-
T8174	6825-6832	NNS	denotes	embryos
T8175	6802-6805	CC	denotes	and
T8176	6806-6813	NN	denotes	p150CAF
T8177	6816-6824	VBN	denotes	depleted
T8178	6813-6814	HYPH	denotes	-
T8179	6814-6815	CD	denotes	1
T8180	6815-6816	HYPH	denotes	-
T8181	6832-6833	.	denotes	.
T8182	6833-7030	sentence	denotes	A loss of p150CAF-1 staining could not be detected before the eight-cell stage (unpublished data) suggesting that maternally contributed protein is present in the embryo up to the four-cell stage.
T8183	6834-6835	DT	denotes	A
T8184	6836-6840	NN	denotes	loss
T8185	6876-6884	VBN	denotes	detected
T8186	6841-6843	IN	denotes	of
T8187	6844-6851	NN	denotes	p150CAF
T8188	6854-6862	NN	denotes	staining
T8189	6851-6852	HYPH	denotes	-
T8190	6852-6853	CD	denotes	1
T8191	6863-6868	MD	denotes	could
T8192	6869-6872	RB	denotes	not
T8193	6873-6875	VB	denotes	be
T8194	6885-6891	IN	denotes	before
T8195	6892-6895	DT	denotes	the
T8196	6907-6912	NN	denotes	stage
T8197	6896-6901	CD	denotes	eight
T8198	6902-6906	NN	denotes	cell
T8199	6901-6902	HYPH	denotes	-
T8200	6913-6914	-LRB-	denotes	(
T8201	6926-6930	NNS	denotes	data
T8202	6914-6925	JJ	denotes	unpublished
T8203	6930-6931	-RRB-	denotes	)
T8204	6932-6942	VBG	denotes	suggesting
T8205	6943-6947	IN	denotes	that
T8206	6979-6981	VBZ	denotes	is
T8207	6948-6958	RB	denotes	maternally
T8208	6959-6970	VBN	denotes	contributed
T8209	6971-6978	NN	denotes	protein
T8210	6982-6989	JJ	denotes	present
T8211	6990-6992	IN	denotes	in
T8212	6993-6996	DT	denotes	the
T8213	6997-7003	NN	denotes	embryo
T8214	7004-7006	IN	denotes	up
T8215	7007-7009	IN	denotes	to
T8216	7010-7013	DT	denotes	the
T8217	7024-7029	NN	denotes	stage
T8218	7014-7018	CD	denotes	four
T8219	7019-7023	NN	denotes	cell
T8220	7018-7019	HYPH	denotes	-
T8221	7029-7030	.	denotes	.
T8222	7030-7162	sentence	denotes	Thus, depletion of p150CAF-1 protein from this stage allows a maximum of two additional cell divisions before developmental arrest.
T8223	7031-7035	RB	denotes	Thus
T8224	7084-7090	VBZ	denotes	allows
T8225	7035-7037	,	denotes	,
T8226	7037-7046	NN	denotes	depletion
T8227	7047-7049	IN	denotes	of
T8228	7050-7057	NN	denotes	p150CAF
T8229	7060-7067	NN	denotes	protein
T8230	7057-7058	HYPH	denotes	-
T8231	7058-7059	CD	denotes	1
T8232	7068-7072	IN	denotes	from
T8233	7073-7077	DT	denotes	this
T8234	7078-7083	NN	denotes	stage
T8235	7091-7092	DT	denotes	a
T8236	7093-7100	NN	denotes	maximum
T8237	7101-7103	IN	denotes	of
T8238	7104-7107	CD	denotes	two
T8239	7124-7133	NNS	denotes	divisions
T8240	7108-7118	JJ	denotes	additional
T8241	7119-7123	NN	denotes	cell
T8242	7134-7140	IN	denotes	before
T8243	7141-7154	JJ	denotes	developmental
T8244	7155-7161	NN	denotes	arrest
T8245	7161-7162	.	denotes	.
T8246	7162-9849	sentence	denotes	Figure 1 Loss of p150CAF-1 Function Leads to Early Developmental Arrest and Alteration of Heterochromatin Organization (A) Generation of Chaf1a +/− mice by homologous recombination in ES cells. (Top) Scheme of the p150CAF1 protein indicating the interacting domains (ID) for HP1 and p60CAF-1, and the acidic domain (AD). (Middle) Structure of the murine Chaf1a gene. Blocks and lines represent exons and introns, respectively. A star indicates the translation initiation site. Below is shown the Chaf1a targeting vector, which includes genomic DNA homology regions (5 HR and 3 HR), the diphtheria toxin (DT), and neomycin selection genes. Recombined (Rec) ES cells and mice were identified by Southern blot using ScaI (S) and the indicated probe. (B) Breeding strategy used for the production of E4 Chaf1a −/− embryos. Genotyping was performed by PCR using the three oligonucleotide primers indicated in (A) (1, 2, and 3). An example of the result of a genotyping experiment is shown. (C) Immunodetection of p150CAF-1 (green) and HP1α (red) in E4 embryos derived from Chaf1a +/− intercrosses. Because Chaf1a +/+ and Chaf1a +/− embryos both stain positively for the presence of p150CAF-1, they are both designated as wild-type. p150CAF-1 expression, which indicates ongoing S phase, can be detected in most cells within the wild-type blastocyst (upper panel). The lower panel shows a 12-cell embryo labeling negatively for p150CAF-1. Only nonspecific background labeling can be observed. In each panel, the right-hand image shows the merge between the HP1α fluorescence and DAPI-stained DNA in blue. Pink color indicates the association of HP1 with DAPI-dense material. The bottom of each panel shows the magnification of a nucleus selected from the above embryo (white square). The arrowhead indicates the typical heterochromatin foci revealed by DAPI and HP1α staining in wild-type embryos. These foci are not visible in Chaf1a −/− embryos. DAPI and HP1α staining are diffuse within the nucleus of p150CAF-1-depleted embryos, with enrichment at the nuclear periphery, revealing abnormal heterochromatin organization. Scale bars represent 10 μm. (E) Wild-type embryos isolated at embryonic day 2 (E2, two cells), E2.5 (four cells), E3 (eight cells), E3.5 (16 cells), and E4 (32 cells) are shown. Heterochromatin was monitored by DAPI staining (upper panel) and HP1 immunolabeling (in red, lower panel). (F) Magnification of a nucleus (DAPI-stained) representative of each stage. The right-hand panel shows the nucleus of an ES cell. Scale bar represents 10 μm. Strikingly, we found that the nuclear organization of heterochromatin appeared abnormal in the nuclei of Chaf1a −/− embryos.
T8247	9725-9735	RB	denotes	Strikingly
T8248	9740-9745	VBD	denotes	found
T8249	9735-9737	,	denotes	,
T8250	9737-9739	PRP	denotes	we
T8251	9746-9750	IN	denotes	that
T8252	9795-9803	VBD	denotes	appeared
T8253	9751-9754	DT	denotes	the
T8254	9763-9775	NN	denotes	organization
T8255	9755-9762	JJ	denotes	nuclear
T8256	9776-9778	IN	denotes	of
T8257	9779-9794	NN	denotes	heterochromatin
T8258	9804-9812	JJ	denotes	abnormal
T8259	9813-9815	IN	denotes	in
T8260	9816-9819	DT	denotes	the
T8261	9820-9826	NNS	denotes	nuclei
T8262	9827-9829	IN	denotes	of
T8263	9830-9836	NN	denotes	Chaf1a
T8264	9841-9848	NNS	denotes	embryos
T8265	9837-9838	SYM	denotes	−
T8266	9838-9839	HYPH	denotes	/
T8267	9839-9840	SYM	denotes	−
T8268	9848-9849	.	denotes	.
T8269	9849-10046	sentence	denotes	Pericentric heterochromatin, the major component of constitutive heterochromatin, can easily be visualized in interphase nuclei by the fluorochrome DAPI and by immunostaining with HP1α [16,17–19].
T8270	9850-9861	JJ	denotes	Pericentric
T8271	9862-9877	NN	denotes	heterochromatin
T8272	9946-9956	VBN	denotes	visualized
T8273	9877-9879	,	denotes	,
T8274	9879-9882	DT	denotes	the
T8275	9889-9898	NN	denotes	component
T8276	9883-9888	JJ	denotes	major
T8277	9899-9901	IN	denotes	of
T8278	9902-9914	JJ	denotes	constitutive
T8279	9915-9930	NN	denotes	heterochromatin
T8280	9930-9932	,	denotes	,
T8281	9932-9935	MD	denotes	can
T8282	9936-9942	RB	denotes	easily
T8283	9943-9945	VB	denotes	be
T8284	9957-9959	IN	denotes	in
T8285	9960-9970	NN	denotes	interphase
T8286	9971-9977	NNS	denotes	nuclei
T8287	9978-9980	IN	denotes	by
T8288	9981-9984	DT	denotes	the
T8289	9998-10002	NN	denotes	DAPI
T8290	9985-9997	NN	denotes	fluorochrome
T8291	10003-10006	CC	denotes	and
T8292	10007-10009	IN	denotes	by
T8293	10010-10024	VBG	denotes	immunostaining
T8294	10025-10029	IN	denotes	with
T8295	10030-10034	NN	denotes	HP1α
T8296	10035-10036	-LRB-	denotes	[
T8297	10036-10038	CD	denotes	16
T8298	10038-10039	,	denotes	,
T8299	10039-10041	CD	denotes	17
T8300	10041-10042	SYM	denotes	–
T8301	10042-10044	CD	denotes	19
T8302	10044-10045	-RRB-	denotes	]
T8303	10045-10046	.	denotes	.
T8304	10046-10275	sentence	denotes	In wild-type blastocysts and in mouse somatic cells, pericentric heterochromatin domains cluster together and form higher-order chromatin structures called chromocenters [20], which are visualized as DAPI-dense foci (Figure 1C).
T8305	10047-10049	IN	denotes	In
T8306	10136-10143	VBP	denotes	cluster
T8307	10050-10054	JJ	denotes	wild
T8308	10055-10059	NN	denotes	type
T8309	10054-10055	HYPH	denotes	-
T8310	10060-10071	NNS	denotes	blastocysts
T8311	10072-10075	CC	denotes	and
T8312	10076-10078	IN	denotes	in
T8313	10079-10084	NN	denotes	mouse
T8314	10093-10098	NNS	denotes	cells
T8315	10085-10092	JJ	denotes	somatic
T8316	10098-10100	,	denotes	,
T8317	10100-10111	JJ	denotes	pericentric
T8318	10128-10135	NNS	denotes	domains
T8319	10112-10127	NN	denotes	heterochromatin
T8320	10144-10152	RB	denotes	together
T8321	10153-10156	CC	denotes	and
T8322	10157-10161	VBP	denotes	form
T8323	10162-10168	JJR	denotes	higher
T8324	10169-10174	NN	denotes	order
T8325	10168-10169	HYPH	denotes	-
T8326	10185-10195	NNS	denotes	structures
T8327	10175-10184	NN	denotes	chromatin
T8328	10196-10202	VBN	denotes	called
T8329	10203-10216	NNS	denotes	chromocenters
T8330	10217-10218	-LRB-	denotes	[
T8331	10218-10220	CD	denotes	20
T8332	10220-10221	-RRB-	denotes	]
T8333	10221-10223	,	denotes	,
T8334	10223-10228	WDT	denotes	which
T8335	10233-10243	VBN	denotes	visualized
T8336	10229-10232	VBP	denotes	are
T8337	10244-10246	IN	denotes	as
T8338	10247-10251	NN	denotes	DAPI
T8339	10252-10257	JJ	denotes	dense
T8340	10251-10252	HYPH	denotes	-
T8341	10258-10262	NNS	denotes	foci
T8342	10263-10264	-LRB-	denotes	(
T8343	10271-10273	NN	denotes	1C
T8344	10264-10270	NN	denotes	Figure
T8345	10273-10274	-RRB-	denotes	)
T8346	10274-10275	.	denotes	.
T8347	10275-10345	sentence	denotes	These structures were not detected in Chaf1a −/− embryos (Figure 1D).
T8348	10276-10281	DT	denotes	These
T8349	10282-10292	NNS	denotes	structures
T8350	10302-10310	VBN	denotes	detected
T8351	10293-10297	VBD	denotes	were
T8352	10298-10301	RB	denotes	not
T8353	10311-10313	IN	denotes	in
T8354	10314-10320	NN	denotes	Chaf1a
T8355	10325-10332	NNS	denotes	embryos
T8356	10321-10322	SYM	denotes	−
T8357	10322-10323	HYPH	denotes	/
T8358	10323-10324	SYM	denotes	−
T8359	10333-10334	-LRB-	denotes	(
T8360	10341-10343	NN	denotes	1D
T8361	10334-10340	NN	denotes	Figure
T8362	10343-10344	-RRB-	denotes	)
T8363	10344-10345	.	denotes	.
T8364	10345-10500	sentence	denotes	Since chromatin architecture and higher-order structures are important for genome function [21], we decided to characterize this phenotype in more detail.
T8365	10346-10351	IN	denotes	Since
T8366	10403-10406	VBP	denotes	are
T8367	10352-10361	NN	denotes	chromatin
T8368	10362-10374	NN	denotes	architecture
T8369	10375-10378	CC	denotes	and
T8370	10379-10385	JJR	denotes	higher
T8371	10386-10391	NN	denotes	order
T8372	10385-10386	HYPH	denotes	-
T8373	10392-10402	NNS	denotes	structures
T8374	10446-10453	VBD	denotes	decided
T8375	10407-10416	JJ	denotes	important
T8376	10417-10420	IN	denotes	for
T8377	10421-10427	NN	denotes	genome
T8378	10428-10436	NN	denotes	function
T8379	10437-10438	-LRB-	denotes	[
T8380	10438-10440	CD	denotes	21
T8381	10440-10441	-RRB-	denotes	]
T8382	10441-10443	,	denotes	,
T8383	10443-10445	PRP	denotes	we
T8384	10454-10456	TO	denotes	to
T8385	10457-10469	VB	denotes	characterize
T8386	10470-10474	DT	denotes	this
T8387	10475-10484	NN	denotes	phenotype
T8388	10485-10487	IN	denotes	in
T8389	10488-10492	JJR	denotes	more
T8390	10493-10499	NN	denotes	detail
T8391	10499-10500	.	denotes	.
T8392	10500-10617	sentence	denotes	For this purpose, we examined the status of heterochromatin organization between the two-cell and blastocyst stages.
T8393	10501-10504	IN	denotes	For
T8394	10522-10530	VBD	denotes	examined
T8395	10505-10509	DT	denotes	this
T8396	10510-10517	NN	denotes	purpose
T8397	10517-10519	,	denotes	,
T8398	10519-10521	PRP	denotes	we
T8399	10531-10534	DT	denotes	the
T8400	10535-10541	NN	denotes	status
T8401	10542-10544	IN	denotes	of
T8402	10545-10560	NN	denotes	heterochromatin
T8403	10561-10573	NN	denotes	organization
T8404	10574-10581	IN	denotes	between
T8405	10582-10585	DT	denotes	the
T8406	10610-10616	NNS	denotes	stages
T8407	10586-10589	CD	denotes	two
T8408	10590-10594	NN	denotes	cell
T8409	10589-10590	HYPH	denotes	-
T8410	10595-10598	CC	denotes	and
T8411	10599-10609	NN	denotes	blastocyst
T8412	10616-10617	.	denotes	.
T8413	10617-10776	sentence	denotes	In two-cell stage wild-type embryos, DAPI staining is diffuse and fibrillar, with regions of higher density around the nucleolar precursor bodies (Figure 1E).
T8414	10618-10620	IN	denotes	In
T8415	10669-10671	VBZ	denotes	is
T8416	10621-10624	CD	denotes	two
T8417	10625-10629	NN	denotes	cell
T8418	10624-10625	HYPH	denotes	-
T8419	10630-10635	NN	denotes	stage
T8420	10646-10653	NNS	denotes	embryos
T8421	10636-10640	JJ	denotes	wild
T8422	10641-10645	NN	denotes	type
T8423	10640-10641	HYPH	denotes	-
T8424	10653-10655	,	denotes	,
T8425	10655-10659	NN	denotes	DAPI
T8426	10660-10668	NN	denotes	staining
T8427	10672-10679	JJ	denotes	diffuse
T8428	10680-10683	CC	denotes	and
T8429	10684-10693	JJ	denotes	fibrillar
T8430	10693-10695	,	denotes	,
T8431	10695-10699	IN	denotes	with
T8432	10700-10707	NNS	denotes	regions
T8433	10708-10710	IN	denotes	of
T8434	10711-10717	JJR	denotes	higher
T8435	10718-10725	NN	denotes	density
T8436	10726-10732	IN	denotes	around
T8437	10733-10736	DT	denotes	the
T8438	10757-10763	NNS	denotes	bodies
T8439	10737-10746	JJ	denotes	nucleolar
T8440	10747-10756	NN	denotes	precursor
T8441	10764-10765	-LRB-	denotes	(
T8442	10772-10774	NN	denotes	1E
T8443	10765-10771	NN	denotes	Figure
T8444	10774-10775	-RRB-	denotes	)
T8445	10775-10776	.	denotes	.
T8446	10776-10914	sentence	denotes	Heterochromatin domains are progressively assembled into DAPI-dense foci between the four-cell and 32-cell blastocyst stages (Figure 1F).
T8447	10777-10792	NN	denotes	Heterochromatin
T8448	10793-10800	NNS	denotes	domains
T8449	10819-10828	VBN	denotes	assembled
T8450	10801-10804	VBP	denotes	are
T8451	10805-10818	RB	denotes	progressively
T8452	10829-10833	IN	denotes	into
T8453	10834-10838	NN	denotes	DAPI
T8454	10839-10844	JJ	denotes	dense
T8455	10838-10839	HYPH	denotes	-
T8456	10845-10849	NNS	denotes	foci
T8457	10850-10857	IN	denotes	between
T8458	10858-10861	DT	denotes	the
T8459	10895-10901	NNS	denotes	stages
T8460	10862-10866	CD	denotes	four
T8461	10867-10871	NN	denotes	cell
T8462	10866-10867	HYPH	denotes	-
T8463	10872-10875	CC	denotes	and
T8464	10876-10878	CD	denotes	32
T8465	10879-10883	NN	denotes	cell
T8466	10878-10879	HYPH	denotes	-
T8467	10884-10894	NN	denotes	blastocyst
T8468	10902-10903	-LRB-	denotes	(
T8469	10910-10912	NN	denotes	1F
T8470	10903-10909	NN	denotes	Figure
T8471	10912-10913	-RRB-	denotes	)
T8472	10913-10914	.	denotes	.
T8473	10914-11049	sentence	denotes	Localization of HP1α confirmed that the structures visualized by DAPI staining correspond to constitutive heterochromatin (Figure 1E).
T8474	10915-10927	NN	denotes	Localization
T8475	10936-10945	VBD	denotes	confirmed
T8476	10928-10930	IN	denotes	of
T8477	10931-10935	NN	denotes	HP1α
T8478	10946-10950	IN	denotes	that
T8479	10994-11004	VBP	denotes	correspond
T8480	10951-10954	DT	denotes	the
T8481	10955-10965	NNS	denotes	structures
T8482	10966-10976	VBN	denotes	visualized
T8483	10977-10979	IN	denotes	by
T8484	10980-10984	NN	denotes	DAPI
T8485	10985-10993	NN	denotes	staining
T8486	11005-11007	IN	denotes	to
T8487	11008-11020	JJ	denotes	constitutive
T8488	11021-11036	NN	denotes	heterochromatin
T8489	11037-11038	-LRB-	denotes	(
T8490	11045-11047	NN	denotes	1E
T8491	11038-11044	NN	denotes	Figure
T8492	11047-11048	-RRB-	denotes	)
T8493	11048-11049	.	denotes	.
T8494	11049-11205	sentence	denotes	Thus, the nuclear organization of heterochromatin is dramatically modified during pre-implantation development, between the two-cell and blastocyst stages.
T8495	11050-11054	RB	denotes	Thus
T8496	11116-11124	VBN	denotes	modified
T8497	11054-11056	,	denotes	,
T8498	11056-11059	DT	denotes	the
T8499	11068-11080	NN	denotes	organization
T8500	11060-11067	JJ	denotes	nuclear
T8501	11081-11083	IN	denotes	of
T8502	11084-11099	NN	denotes	heterochromatin
T8503	11100-11102	VBZ	denotes	is
T8504	11103-11115	RB	denotes	dramatically
T8505	11125-11131	IN	denotes	during
T8506	11132-11148	JJ	denotes	pre-implantation
T8507	11149-11160	NN	denotes	development
T8508	11160-11162	,	denotes	,
T8509	11162-11169	IN	denotes	between
T8510	11170-11173	DT	denotes	the
T8511	11198-11204	NNS	denotes	stages
T8512	11174-11177	CD	denotes	two
T8513	11178-11182	NN	denotes	cell
T8514	11177-11178	HYPH	denotes	-
T8515	11183-11186	CC	denotes	and
T8516	11187-11197	NN	denotes	blastocyst
T8517	11204-11205	.	denotes	.
T8518	11205-11340	sentence	denotes	In Chaf1a −/− E4 embryos, which are arrested between the eight- and 16-cell stage, DAPI-dense foci were barely detectable (Figure 1D).
T8519	11206-11208	IN	denotes	In
T8520	11305-11309	VBD	denotes	were
T8521	11209-11215	NN	denotes	Chaf1a
T8522	11223-11230	NNS	denotes	embryos
T8523	11216-11217	SYM	denotes	−
T8524	11217-11218	HYPH	denotes	/
T8525	11218-11219	SYM	denotes	−
T8526	11220-11222	NN	denotes	E4
T8527	11230-11232	,	denotes	,
T8528	11232-11237	WDT	denotes	which
T8529	11242-11250	VBN	denotes	arrested
T8530	11238-11241	VBP	denotes	are
T8531	11251-11258	IN	denotes	between
T8532	11259-11262	DT	denotes	the
T8533	11282-11287	NN	denotes	stage
T8534	11263-11268	CD	denotes	eight
T8535	11277-11281	NN	denotes	cell
T8536	11268-11269	HYPH	denotes	-
T8537	11270-11273	CC	denotes	and
T8539	11276-11277	HYPH	denotes	-
T8540	11287-11289	,	denotes	,
T8541	11289-11293	NN	denotes	DAPI
T8542	11294-11299	JJ	denotes	dense
T8543	11293-11294	HYPH	denotes	-
T8544	11300-11304	NNS	denotes	foci
T8545	11310-11316	RB	denotes	barely
T8546	11317-11327	JJ	denotes	detectable
T8547	11328-11329	-LRB-	denotes	(
T8548	11336-11338	NN	denotes	1D
T8549	11329-11335	NN	denotes	Figure
T8550	11338-11339	-RRB-	denotes	)
T8551	11339-11340	.	denotes	.
T8552	11340-11494	sentence	denotes	Instead, DAPI staining was diffuse within the nucleus, with regions of higher density around the nucleoli and at the periphery of the nuclei (Figure 1D).
T8553	11341-11348	RB	denotes	Instead
T8554	11364-11367	VBD	denotes	was
T8555	11348-11350	,	denotes	,
T8556	11350-11354	NN	denotes	DAPI
T8557	11355-11363	NN	denotes	staining
T8558	11368-11375	JJ	denotes	diffuse
T8559	11376-11382	IN	denotes	within
T8560	11383-11386	DT	denotes	the
T8561	11387-11394	NN	denotes	nucleus
T8562	11394-11396	,	denotes	,
T8563	11396-11400	IN	denotes	with
T8564	11401-11408	NNS	denotes	regions
T8565	11409-11411	IN	denotes	of
T8566	11412-11418	JJR	denotes	higher
T8567	11419-11426	NN	denotes	density
T8568	11427-11433	IN	denotes	around
T8569	11434-11437	DT	denotes	the
T8570	11438-11446	NNS	denotes	nucleoli
T8571	11447-11450	CC	denotes	and
T8572	11451-11453	IN	denotes	at
T8573	11454-11457	DT	denotes	the
T8574	11458-11467	NN	denotes	periphery
T8575	11468-11470	IN	denotes	of
T8576	11471-11474	DT	denotes	the
T8577	11475-11481	NNS	denotes	nuclei
T8578	11482-11483	-LRB-	denotes	(
T8579	11490-11492	NN	denotes	1D
T8580	11483-11489	NN	denotes	Figure
T8581	11492-11493	-RRB-	denotes	)
T8582	11493-11494	.	denotes	.
T8583	11494-11645	sentence	denotes	Localization of HP1α showed a diffuse pattern similar to the DAPI, with some enrichment at the nuclear periphery, and around the nucleoli (Figure 1D).
T8584	11495-11507	NN	denotes	Localization
T8585	11516-11522	VBD	denotes	showed
T8586	11508-11510	IN	denotes	of
T8587	11511-11515	NN	denotes	HP1α
T8588	11523-11524	DT	denotes	a
T8589	11533-11540	NN	denotes	pattern
T8590	11525-11532	JJ	denotes	diffuse
T8591	11541-11548	JJ	denotes	similar
T8592	11549-11551	IN	denotes	to
T8593	11552-11555	DT	denotes	the
T8594	11556-11560	NN	denotes	DAPI
T8595	11560-11562	,	denotes	,
T8596	11562-11566	IN	denotes	with
T8597	11567-11571	DT	denotes	some
T8598	11572-11582	NN	denotes	enrichment
T8599	11583-11585	IN	denotes	at
T8600	11586-11589	DT	denotes	the
T8601	11598-11607	NN	denotes	periphery
T8602	11590-11597	JJ	denotes	nuclear
T8603	11607-11609	,	denotes	,
T8604	11609-11612	CC	denotes	and
T8605	11613-11619	IN	denotes	around
T8606	11620-11623	DT	denotes	the
T8607	11624-11632	NNS	denotes	nucleoli
T8608	11633-11634	-LRB-	denotes	(
T8609	11641-11643	NN	denotes	1D
T8610	11634-11640	NN	denotes	Figure
T8611	11643-11644	-RRB-	denotes	)
T8612	11644-11645	.	denotes	.
T8613	11645-11843	sentence	denotes	This abnormal organization of heterochromatin in Chaf1a −/− embryos is reminiscent of the heterochromatin organization found in two- to four-cell stage wild-type embryos (compare Figure 1D and 1F).
T8614	11646-11650	DT	denotes	This
T8615	11660-11672	NN	denotes	organization
T8616	11651-11659	JJ	denotes	abnormal
T8617	11714-11716	VBZ	denotes	is
T8618	11673-11675	IN	denotes	of
T8619	11676-11691	NN	denotes	heterochromatin
T8620	11692-11694	IN	denotes	in
T8621	11695-11701	NN	denotes	Chaf1a
T8622	11706-11713	NNS	denotes	embryos
T8623	11702-11703	SYM	denotes	−
T8624	11703-11704	HYPH	denotes	/
T8625	11704-11705	SYM	denotes	−
T8626	11717-11728	JJ	denotes	reminiscent
T8627	11729-11731	IN	denotes	of
T8628	11732-11735	DT	denotes	the
T8629	11752-11764	NN	denotes	organization
T8630	11736-11751	NN	denotes	heterochromatin
T8631	11765-11770	VBN	denotes	found
T8632	11771-11773	IN	denotes	in
T8633	11774-11777	CD	denotes	two
T8634	11782-11786	CD	denotes	four
T8635	11777-11778	HYPH	denotes	-
T8636	11779-11781	IN	denotes	to
T8637	11787-11791	NN	denotes	cell
T8638	11786-11787	HYPH	denotes	-
T8639	11792-11797	NN	denotes	stage
T8640	11808-11815	NNS	denotes	embryos
T8641	11798-11802	JJ	denotes	wild
T8642	11803-11807	NN	denotes	type
T8643	11802-11803	HYPH	denotes	-
T8644	11816-11817	-LRB-	denotes	(
T8645	11817-11824	VB	denotes	compare
T8646	11825-11831	NN	denotes	Figure
T8647	11832-11834	NN	denotes	1D
T8648	11835-11838	CC	denotes	and
T8649	11839-11841	NN	denotes	1F
T8650	11841-11842	-RRB-	denotes	)
T8651	11842-11843	.	denotes	.
T8652	11843-12047	sentence	denotes	These data evidence a key role for p150CAF-1 during pre-implantation development, and reveal that this protein is required for the proper 3-D organization of heterochromatin within embryonic cell nuclei.
T8653	11844-11849	DT	denotes	These
T8654	11850-11854	NNS	denotes	data
T8655	11855-11863	VBP	denotes	evidence
T8656	11864-11865	DT	denotes	a
T8657	11870-11874	NN	denotes	role
T8658	11866-11869	JJ	denotes	key
T8659	11875-11878	IN	denotes	for
T8660	11879-11886	NN	denotes	p150CAF
T8661	11886-11887	HYPH	denotes	-
T8662	11887-11888	CD	denotes	1
T8663	11889-11895	IN	denotes	during
T8664	11896-11912	JJ	denotes	pre-implantation
T8665	11913-11924	NN	denotes	development
T8666	11924-11926	,	denotes	,
T8667	11926-11929	CC	denotes	and
T8668	11930-11936	VBP	denotes	reveal
T8669	11937-11941	IN	denotes	that
T8670	11958-11966	VBN	denotes	required
T8671	11942-11946	DT	denotes	this
T8672	11947-11954	NN	denotes	protein
T8673	11955-11957	VBZ	denotes	is
T8674	11967-11970	IN	denotes	for
T8675	11971-11974	DT	denotes	the
T8676	11986-11998	NN	denotes	organization
T8677	11975-11981	JJ	denotes	proper
T8678	11982-11983	CD	denotes	3
T8679	11984-11985	NN	denotes	D
T8680	11983-11984	HYPH	denotes	-
T8681	11999-12001	IN	denotes	of
T8682	12002-12017	NN	denotes	heterochromatin
T8683	12018-12024	IN	denotes	within
T8684	12025-12034	JJ	denotes	embryonic
T8685	12040-12046	NNS	denotes	nuclei
T8686	12035-12039	NN	denotes	cell
T8687	12046-12047	.	denotes	.
T8688	12047-12196	sentence	denotes	Next, we wondered whether a similar requirement for CAF-1 could also be observed in ES cells, which are derived from the blastocyst inner cell mass.
T8689	12048-12052	RB	denotes	Next
T8690	12057-12065	VBD	denotes	wondered
T8691	12052-12054	,	denotes	,
T8692	12054-12056	PRP	denotes	we
T8693	12066-12073	IN	denotes	whether
T8694	12120-12128	VBN	denotes	observed
T8695	12074-12075	DT	denotes	a
T8696	12084-12095	NN	denotes	requirement
T8697	12076-12083	JJ	denotes	similar
T8698	12096-12099	IN	denotes	for
T8699	12100-12103	NN	denotes	CAF
T8700	12103-12104	HYPH	denotes	-
T8701	12104-12105	CD	denotes	1
T8702	12106-12111	MD	denotes	could
T8703	12112-12116	RB	denotes	also
T8704	12117-12119	VB	denotes	be
T8705	12129-12131	IN	denotes	in
T8706	12132-12134	NN	denotes	ES
T8707	12135-12140	NNS	denotes	cells
T8708	12140-12142	,	denotes	,
T8709	12142-12147	WDT	denotes	which
T8710	12152-12159	VBN	denotes	derived
T8711	12148-12151	VBP	denotes	are
T8712	12160-12164	IN	denotes	from
T8713	12165-12168	DT	denotes	the
T8714	12191-12195	NN	denotes	mass
T8715	12169-12179	NN	denotes	blastocyst
T8716	12180-12185	JJ	denotes	inner
T8717	12186-12190	NN	denotes	cell
T8718	12195-12196	.	denotes	.
T8719	12196-12372	sentence	denotes	Given the early developmental arrest observed in Chaf1a −/− embryos, such cells could not be derived directly from null embryos, and we thus used an RNAi strategy (Figure 2A).
T8720	12197-12202	VBN	denotes	Given
T8721	12290-12297	VBN	denotes	derived
T8722	12203-12206	DT	denotes	the
T8723	12227-12233	NN	denotes	arrest
T8724	12207-12212	JJ	denotes	early
T8725	12213-12226	JJ	denotes	developmental
T8726	12234-12242	VBN	denotes	observed
T8727	12243-12245	IN	denotes	in
T8728	12246-12252	NN	denotes	Chaf1a
T8729	12257-12264	NNS	denotes	embryos
T8730	12253-12254	SYM	denotes	−
T8731	12254-12255	HYPH	denotes	/
T8732	12255-12256	SYM	denotes	−
T8733	12264-12266	,	denotes	,
T8734	12266-12270	JJ	denotes	such
T8735	12271-12276	NNS	denotes	cells
T8736	12277-12282	MD	denotes	could
T8737	12283-12286	RB	denotes	not
T8738	12287-12289	VB	denotes	be
T8739	12298-12306	RB	denotes	directly
T8740	12307-12311	IN	denotes	from
T8741	12312-12316	JJ	denotes	null
T8742	12317-12324	NNS	denotes	embryos
T8743	12324-12326	,	denotes	,
T8744	12326-12329	CC	denotes	and
T8745	12330-12332	PRP	denotes	we
T8746	12338-12342	VBD	denotes	used
T8747	12333-12337	RB	denotes	thus
T8748	12343-12345	DT	denotes	an
T8749	12351-12359	NN	denotes	strategy
T8750	12346-12350	NN	denotes	RNAi
T8751	12360-12361	-LRB-	denotes	(
T8752	12368-12370	NN	denotes	2A
T8753	12361-12367	NN	denotes	Figure
T8754	12370-12371	-RRB-	denotes	)
T8755	12371-12372	.	denotes	.
T8756	12372-12452	sentence	denotes	p150CAF-1 knockdown in ES cells was quantified by Western blot analysis and IF.
T8757	12373-12380	NN	denotes	p150CAF
T8758	12383-12392	NN	denotes	knockdown
T8759	12380-12381	HYPH	denotes	-
T8760	12381-12382	CD	denotes	1
T8761	12409-12419	VBN	denotes	quantified
T8762	12393-12395	IN	denotes	in
T8763	12396-12398	NN	denotes	ES
T8764	12399-12404	NNS	denotes	cells
T8765	12405-12408	VBD	denotes	was
T8766	12420-12422	IN	denotes	by
T8767	12423-12430	NNP	denotes	Western
T8768	12431-12435	NN	denotes	blot
T8769	12436-12444	NN	denotes	analysis
T8770	12445-12448	CC	denotes	and
T8771	12449-12451	NN	denotes	IF
T8772	12451-12452	.	denotes	.
T8773	12452-12465	sentence	denotes	(Figure S1).
T8774	12453-12454	-LRB-	denotes	(
T8775	12461-12463	NN	denotes	S1
T8776	12454-12460	NN	denotes	Figure
T8777	12463-12464	-RRB-	denotes	)
T8778	12464-12465	.	denotes	.
T8779	12465-12569	sentence	denotes	p150CAF-1 RNAi-depleted ES cells displayed a phenotype very similar to the cells of Chaf1a −/− E4 mice.
T8780	12466-12473	NN	denotes	p150CAF
T8781	12493-12498	NNS	denotes	cells
T8782	12473-12474	HYPH	denotes	-
T8783	12474-12475	CD	denotes	1
T8784	12476-12480	NN	denotes	RNAi
T8785	12481-12489	VBN	denotes	depleted
T8786	12480-12481	HYPH	denotes	-
T8787	12490-12492	NN	denotes	ES
T8788	12499-12508	VBD	denotes	displayed
T8789	12509-12510	DT	denotes	a
T8790	12511-12520	NN	denotes	phenotype
T8791	12521-12525	RB	denotes	very
T8792	12526-12533	JJ	denotes	similar
T8793	12534-12536	IN	denotes	to
T8794	12537-12540	DT	denotes	the
T8795	12541-12546	NNS	denotes	cells
T8796	12547-12549	IN	denotes	of
T8797	12550-12556	NN	denotes	Chaf1a
T8798	12564-12568	NNS	denotes	mice
T8799	12557-12558	SYM	denotes	−
T8800	12558-12559	HYPH	denotes	/
T8801	12559-12560	SYM	denotes	−
T8802	12561-12563	NN	denotes	E4
T8803	12568-12569	.	denotes	.
T8804	12569-12712	sentence	denotes	DAPI-dense foci were lost, and we observed diffuse HP1α and DAPI staining around the nucleoli and at the periphery of the nucleus (Figure 2B).
T8805	12570-12574	NN	denotes	DAPI
T8806	12575-12580	JJ	denotes	dense
T8807	12574-12575	HYPH	denotes	-
T8808	12581-12585	NNS	denotes	foci
T8809	12591-12595	VBN	denotes	lost
T8810	12586-12590	VBD	denotes	were
T8811	12595-12597	,	denotes	,
T8812	12597-12600	CC	denotes	and
T8813	12601-12603	PRP	denotes	we
T8814	12604-12612	VBD	denotes	observed
T8815	12613-12620	JJ	denotes	diffuse
T8816	12635-12643	NN	denotes	staining
T8817	12621-12625	NN	denotes	HP1α
T8818	12626-12629	CC	denotes	and
T8819	12630-12634	NN	denotes	DAPI
T8820	12644-12650	IN	denotes	around
T8821	12651-12654	DT	denotes	the
T8822	12655-12663	NNS	denotes	nucleoli
T8823	12664-12667	CC	denotes	and
T8824	12668-12670	IN	denotes	at
T8825	12671-12674	DT	denotes	the
T8826	12675-12684	NN	denotes	periphery
T8827	12685-12687	IN	denotes	of
T8828	12688-12691	DT	denotes	the
T8829	12692-12699	NN	denotes	nucleus
T8830	12700-12701	-LRB-	denotes	(
T8831	12708-12710	NN	denotes	2B
T8832	12701-12707	NN	denotes	Figure
T8833	12710-12711	-RRB-	denotes	)
T8834	12711-12712	.	denotes	.
T8835	12712-12870	sentence	denotes	This result indicates that p150CAF-1 is essential for nuclear organization of heterochromatin in ES cells in a similar way to early pre-implantation embryos.
T8836	12713-12717	DT	denotes	This
T8837	12718-12724	NN	denotes	result
T8838	12725-12734	VBZ	denotes	indicates
T8839	12735-12739	IN	denotes	that
T8840	12750-12752	VBZ	denotes	is
T8841	12740-12747	NN	denotes	p150CAF
T8842	12747-12748	HYPH	denotes	-
T8843	12748-12749	CD	denotes	1
T8844	12753-12762	JJ	denotes	essential
T8845	12763-12766	IN	denotes	for
T8846	12767-12774	JJ	denotes	nuclear
T8847	12775-12787	NN	denotes	organization
T8848	12788-12790	IN	denotes	of
T8849	12791-12806	NN	denotes	heterochromatin
T8850	12807-12809	IN	denotes	in
T8851	12810-12812	NN	denotes	ES
T8852	12813-12818	NNS	denotes	cells
T8853	12819-12821	IN	denotes	in
T8854	12822-12823	DT	denotes	a
T8855	12832-12835	NN	denotes	way
T8856	12824-12831	JJ	denotes	similar
T8857	12836-12838	IN	denotes	to
T8858	12839-12844	JJ	denotes	early
T8859	12862-12869	NNS	denotes	embryos
T8860	12845-12861	JJ	denotes	pre-implantation
T8861	12869-12870	.	denotes	.
T8862	12870-13043	sentence	denotes	Surprisingly, we did not observe this severe alteration in heterochromatin organization in primary mouse embryonic fibroblasts (MEFs) following p150CAF-1 depletion by RNAi.
T8863	12871-12883	RB	denotes	Surprisingly
T8864	12896-12903	VB	denotes	observe
T8865	12883-12885	,	denotes	,
T8866	12885-12887	PRP	denotes	we
T8867	12888-12891	VBD	denotes	did
T8868	12892-12895	RB	denotes	not
T8869	12904-12908	DT	denotes	this
T8870	12916-12926	NN	denotes	alteration
T8871	12909-12915	JJ	denotes	severe
T8872	12927-12929	IN	denotes	in
T8873	12930-12945	NN	denotes	heterochromatin
T8874	12946-12958	NN	denotes	organization
T8875	12959-12961	IN	denotes	in
T8876	12962-12969	JJ	denotes	primary
T8877	12986-12997	NNS	denotes	fibroblasts
T8878	12970-12975	NN	denotes	mouse
T8879	12976-12985	JJ	denotes	embryonic
T8880	12998-12999	-LRB-	denotes	(
T8881	12999-13003	NNS	denotes	MEFs
T8882	13003-13004	-RRB-	denotes	)
T8883	13005-13014	VBG	denotes	following
T8884	13015-13022	NN	denotes	p150CAF
T8885	13025-13034	NN	denotes	depletion
T8886	13022-13023	HYPH	denotes	-
T8887	13023-13024	CD	denotes	1
T8888	13035-13037	IN	denotes	by
T8889	13038-13042	NN	denotes	RNAi
T8890	13042-13043	.	denotes	.
T8891	13043-13226	sentence	denotes	p150CAF-1 depletion in MEFs resulted in a strong inhibition of cell proliferation (unpublished data), but did not alter the clustering of heterochromatin domains (Figures 2C and S2).
T8892	13044-13051	NN	denotes	p150CAF
T8893	13054-13063	NN	denotes	depletion
T8894	13051-13052	HYPH	denotes	-
T8895	13052-13053	CD	denotes	1
T8896	13072-13080	VBD	denotes	resulted
T8897	13064-13066	IN	denotes	in
T8898	13067-13071	NNS	denotes	MEFs
T8899	13081-13083	IN	denotes	in
T8900	13084-13085	DT	denotes	a
T8901	13093-13103	NN	denotes	inhibition
T8902	13086-13092	JJ	denotes	strong
T8903	13104-13106	IN	denotes	of
T8904	13107-13111	NN	denotes	cell
T8905	13112-13125	NN	denotes	proliferation
T8906	13126-13127	-LRB-	denotes	(
T8907	13139-13143	NNS	denotes	data
T8908	13127-13138	JJ	denotes	unpublished
T8909	13143-13144	-RRB-	denotes	)
T8910	13144-13146	,	denotes	,
T8911	13146-13149	CC	denotes	but
T8912	13150-13153	VBD	denotes	did
T8913	13158-13163	VB	denotes	alter
T8914	13154-13157	RB	denotes	not
T8915	13164-13167	DT	denotes	the
T8916	13168-13178	NN	denotes	clustering
T8917	13179-13181	IN	denotes	of
T8918	13182-13197	NN	denotes	heterochromatin
T8919	13198-13205	NNS	denotes	domains
T8920	13206-13207	-LRB-	denotes	(
T8921	13215-13217	NN	denotes	2C
T8922	13207-13214	NNS	denotes	Figures
T8923	13218-13221	CC	denotes	and
T8924	13222-13224	NN	denotes	S2
T8925	13224-13225	-RRB-	denotes	)
T8926	13225-13226	.	denotes	.
T8927	13226-13320	sentence	denotes	Similarly, p150CAF-1 depletion in 3T3 cells did not affect heterochromatin organization [16].
T8928	13227-13236	RB	denotes	Similarly
T8929	13279-13285	VB	denotes	affect
T8930	13236-13238	,	denotes	,
T8931	13238-13245	NN	denotes	p150CAF
T8932	13248-13257	NN	denotes	depletion
T8933	13245-13246	HYPH	denotes	-
T8934	13246-13247	CD	denotes	1
T8935	13258-13260	IN	denotes	in
T8936	13261-13264	NN	denotes	3T3
T8937	13265-13270	NNS	denotes	cells
T8938	13271-13274	VBD	denotes	did
T8939	13275-13278	RB	denotes	not
T8940	13286-13301	NN	denotes	heterochromatin
T8941	13302-13314	NN	denotes	organization
T8942	13315-13316	-LRB-	denotes	[
T8943	13316-13318	CD	denotes	16
T8944	13318-13319	-RRB-	denotes	]
T8945	13319-13320	.	denotes	.
T8946	13320-13410	sentence	denotes	These observations reveal a specific function for CAF-1 in the early embryo and ES cells.
T8947	13321-13326	DT	denotes	These
T8948	13327-13339	NNS	denotes	observations
T8949	13340-13346	VBP	denotes	reveal
T8950	13347-13348	DT	denotes	a
T8951	13358-13366	NN	denotes	function
T8952	13349-13357	JJ	denotes	specific
T8953	13367-13370	IN	denotes	for
T8954	13371-13374	NN	denotes	CAF
T8955	13374-13375	HYPH	denotes	-
T8956	13375-13376	CD	denotes	1
T8957	13377-13379	IN	denotes	in
T8958	13380-13383	DT	denotes	the
T8959	13390-13396	NN	denotes	embryo
T8960	13384-13389	JJ	denotes	early
T8961	13397-13400	CC	denotes	and
T8962	13401-13403	NN	denotes	ES
T8963	13404-13409	NNS	denotes	cells
T8964	13409-13410	.	denotes	.
T8965	13410-14893	sentence	denotes	Figure 2 Depletion of p150CAF-1 in ES Cells Results in a Severe Alteration of Heterochromatin Organization (A) Strategy used to deplete p150CAF-1 by RNAi in ES cells. The siRNA expression vector includes a puromycin selection cassette (Puro), the mouse H1 promoter, and the siRNA encoding sequence. ES cells were kept under puromycin selection during 48 h following transfection. (B) Abnormal heterochromatin organization in p150CAF-1-depleted ES cells. Immunodetection of p150CAF-1 (green) and HP1α (red) in ES cells transfected with control (cont) and p150CAF-1 siRNA. siRNA expression results in efficient p150CAF-1 depletion. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue. Scale bar = 10 μm. (C) Heterochromatin organization is not altered in p150CAF-1-depleted MEFs. Immunodetection of p150CAF-1 (green) and HP1α (red) in MEFs transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue. (D) PML bodies are not altered in p150CAF-1-depleted ES cells. Immunodetection of PML (red) in ES cells transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between PML fluorescence and DAPI-stained DNA in blue. We also wondered whether loss of p150CAF-1 in ES cells specifically affects the heterochromatin nuclear subcompartment, or whether it might result in a more global loss of nuclear organization and architecture.
T8966	14683-14685	PRP	denotes	We
T8967	14691-14699	VBD	denotes	wondered
T8968	14686-14690	RB	denotes	also
T8969	14700-14707	IN	denotes	whether
T8970	14751-14758	VBZ	denotes	affects
T8971	14708-14712	NN	denotes	loss
T8972	14713-14715	IN	denotes	of
T8973	14716-14723	NN	denotes	p150CAF
T8974	14723-14724	HYPH	denotes	-
T8975	14724-14725	CD	denotes	1
T8976	14726-14728	IN	denotes	in
T8977	14729-14731	NN	denotes	ES
T8978	14732-14737	NNS	denotes	cells
T8979	14738-14750	RB	denotes	specifically
T8980	14759-14762	DT	denotes	the
T8981	14787-14801	NN	denotes	subcompartment
T8982	14763-14778	NN	denotes	heterochromatin
T8983	14779-14786	JJ	denotes	nuclear
T8984	14801-14803	,	denotes	,
T8985	14803-14805	CC	denotes	or
T8986	14806-14813	IN	denotes	whether
T8987	14823-14829	VB	denotes	result
T8988	14814-14816	PRP	denotes	it
T8989	14817-14822	MD	denotes	might
T8990	14830-14832	IN	denotes	in
T8991	14833-14834	DT	denotes	a
T8992	14847-14851	NN	denotes	loss
T8993	14835-14839	RBR	denotes	more
T8994	14840-14846	JJ	denotes	global
T8995	14852-14854	IN	denotes	of
T8996	14855-14862	JJ	denotes	nuclear
T8997	14863-14875	NN	denotes	organization
T8998	14876-14879	CC	denotes	and
T8999	14880-14892	NN	denotes	architecture
T9000	14892-14893	.	denotes	.
T9001	14893-15072	sentence	denotes	We used a specific antibody against the promyelocytic leukemia (PML) protein to study the fate of the PML nuclear bodies, which are well-characterized subnuclear structures [22].
T9002	14894-14896	PRP	denotes	We
T9003	14897-14901	VBD	denotes	used
T9004	14902-14903	DT	denotes	a
T9005	14913-14921	NN	denotes	antibody
T9006	14904-14912	JJ	denotes	specific
T9007	14922-14929	IN	denotes	against
T9008	14930-14933	DT	denotes	the
T9009	14963-14970	NN	denotes	protein
T9010	14934-14947	JJ	denotes	promyelocytic
T9011	14948-14956	NN	denotes	leukemia
T9012	14957-14958	-LRB-	denotes	(
T9013	14958-14961	NN	denotes	PML
T9014	14961-14962	-RRB-	denotes	)
T9015	14971-14973	TO	denotes	to
T9016	14974-14979	VB	denotes	study
T9017	14980-14983	DT	denotes	the
T9018	14984-14988	NN	denotes	fate
T9019	14989-14991	IN	denotes	of
T9020	14992-14995	DT	denotes	the
T9021	15008-15014	NNS	denotes	bodies
T9022	14996-14999	NN	denotes	PML
T9023	15000-15007	JJ	denotes	nuclear
T9024	15014-15016	,	denotes	,
T9025	15016-15021	WDT	denotes	which
T9026	15022-15025	VBP	denotes	are
T9027	15026-15030	RB	denotes	well
T9028	15031-15044	VBN	denotes	characterized
T9029	15030-15031	HYPH	denotes	-
T9030	15056-15066	NNS	denotes	structures
T9031	15045-15055	JJ	denotes	subnuclear
T9032	15067-15068	-LRB-	denotes	[
T9033	15068-15070	CD	denotes	22
T9034	15070-15071	-RRB-	denotes	]
T9035	15071-15072	.	denotes	.
T9036	15072-15233	sentence	denotes	We could not detect any significant difference in the distribution and aspect of PML nuclear bodies between control and p150CAF-1-depleted ES cells (Figure 2D).
T9037	15073-15075	PRP	denotes	We
T9038	15086-15092	VB	denotes	detect
T9039	15076-15081	MD	denotes	could
T9040	15082-15085	RB	denotes	not
T9041	15093-15096	DT	denotes	any
T9042	15109-15119	NN	denotes	difference
T9043	15097-15108	JJ	denotes	significant
T9044	15120-15122	IN	denotes	in
T9045	15123-15126	DT	denotes	the
T9046	15127-15139	NN	denotes	distribution
T9047	15140-15143	CC	denotes	and
T9048	15144-15150	NN	denotes	aspect
T9049	15151-15153	IN	denotes	of
T9050	15154-15157	NN	denotes	PML
T9051	15166-15172	NNS	denotes	bodies
T9052	15158-15165	JJ	denotes	nuclear
T9053	15173-15180	IN	denotes	between
T9054	15181-15188	NN	denotes	control
T9055	15215-15220	NNS	denotes	cells
T9056	15189-15192	CC	denotes	and
T9057	15193-15200	NN	denotes	p150CAF
T9058	15203-15211	VBN	denotes	depleted
T9059	15200-15201	HYPH	denotes	-
T9060	15201-15202	CD	denotes	1
T9061	15202-15203	HYPH	denotes	-
T9062	15212-15214	NN	denotes	ES
T9063	15221-15222	-LRB-	denotes	(
T9064	15229-15231	NN	denotes	2D
T9065	15222-15228	NN	denotes	Figure
T9066	15231-15232	-RRB-	denotes	)
T9067	15232-15233	.	denotes	.
T9068	15233-15333	sentence	denotes	These data show that nuclear architecture is not globally altered following CAF-1 loss-of-function.
T9069	15234-15239	DT	denotes	These
T9070	15240-15244	NNS	denotes	data
T9071	15245-15249	VBP	denotes	show
T9072	15250-15254	IN	denotes	that
T9073	15292-15299	VBN	denotes	altered
T9074	15255-15262	JJ	denotes	nuclear
T9075	15263-15275	NN	denotes	architecture
T9076	15276-15278	VBZ	denotes	is
T9077	15279-15282	RB	denotes	not
T9078	15283-15291	RB	denotes	globally
T9079	15300-15309	VBG	denotes	following
T9080	15310-15313	NN	denotes	CAF
T9081	15313-15314	HYPH	denotes	-
T9082	15314-15315	CD	denotes	1
T9083	15316-15320	NN	denotes	loss
T9084	15320-15321	HYPH	denotes	-
T9085	15321-15323	IN	denotes	of
T9086	15323-15324	HYPH	denotes	-
T9087	15324-15332	NN	denotes	function
T9088	15332-15333	.	denotes	.
T9089	15333-15508	sentence	denotes	In addition, Western blot analysis revealed that CAF-1 depletion did not result in altered levels of chromatin architectural proteins such as histone H3 and HP1α (Figure S1).
T9090	15334-15336	IN	denotes	In
T9091	15369-15377	VBD	denotes	revealed
T9092	15337-15345	NN	denotes	addition
T9093	15345-15347	,	denotes	,
T9094	15347-15354	NNP	denotes	Western
T9095	15355-15359	NN	denotes	blot
T9096	15360-15368	NN	denotes	analysis
T9097	15378-15382	IN	denotes	that
T9098	15407-15413	VB	denotes	result
T9099	15383-15386	NN	denotes	CAF
T9100	15389-15398	NN	denotes	depletion
T9101	15386-15387	HYPH	denotes	-
T9102	15387-15388	CD	denotes	1
T9103	15399-15402	VBD	denotes	did
T9104	15403-15406	RB	denotes	not
T9105	15414-15416	IN	denotes	in
T9106	15417-15424	VBN	denotes	altered
T9107	15425-15431	NNS	denotes	levels
T9108	15432-15434	IN	denotes	of
T9109	15435-15444	NN	denotes	chromatin
T9110	15459-15467	NN	denotes	proteins
T9111	15445-15458	JJ	denotes	architectural
T9112	15468-15472	JJ	denotes	such
T9113	15473-15475	IN	denotes	as
T9114	15476-15483	NN	denotes	histone
T9115	15484-15486	NN	denotes	H3
T9116	15487-15490	CC	denotes	and
T9117	15491-15495	NN	denotes	HP1α
T9118	15496-15497	-LRB-	denotes	(
T9119	15504-15506	NN	denotes	S1
T9120	15497-15503	NN	denotes	Figure
T9121	15506-15507	-RRB-	denotes	)
T9122	15507-15508	.	denotes	.
T9123	15508-15653	sentence	denotes	Thus, CAF-1 is specifically required in early embryos and in ES cells for the proper organization of the heterochromatin subnuclear compartment.
T9124	15509-15513	RB	denotes	Thus
T9125	15537-15545	VBN	denotes	required
T9126	15513-15515	,	denotes	,
T9127	15515-15518	NN	denotes	CAF
T9128	15518-15519	HYPH	denotes	-
T9129	15519-15520	CD	denotes	1
T9130	15521-15523	VBZ	denotes	is
T9131	15524-15536	RB	denotes	specifically
T9132	15546-15548	IN	denotes	in
T9133	15549-15554	JJ	denotes	early
T9134	15555-15562	NNS	denotes	embryos
T9135	15563-15566	CC	denotes	and
T9136	15567-15569	IN	denotes	in
T9137	15570-15572	NN	denotes	ES
T9138	15573-15578	NNS	denotes	cells
T9139	15579-15582	IN	denotes	for
T9140	15583-15586	DT	denotes	the
T9141	15594-15606	NN	denotes	organization
T9142	15587-15593	JJ	denotes	proper
T9143	15607-15609	IN	denotes	of
T9144	15610-15613	DT	denotes	the
T9145	15641-15652	NN	denotes	compartment
T9146	15614-15629	NN	denotes	heterochromatin
T9147	15630-15640	JJ	denotes	subnuclear
T9148	15652-15653	.	denotes	.
T9149	15653-15779	sentence	denotes	Previous studies performed in mammalian cell lines showed that CAF-1 activity is required for S phase progression [16,23,24].
T9150	15654-15662	JJ	denotes	Previous
T9151	15663-15670	NNS	denotes	studies
T9152	15705-15711	VBD	denotes	showed
T9153	15671-15680	VBN	denotes	performed
T9154	15681-15683	IN	denotes	in
T9155	15684-15693	JJ	denotes	mammalian
T9156	15699-15704	NNS	denotes	lines
T9157	15694-15698	NN	denotes	cell
T9158	15712-15716	IN	denotes	that
T9159	15735-15743	VBN	denotes	required
T9160	15717-15720	NN	denotes	CAF
T9161	15723-15731	NN	denotes	activity
T9162	15720-15721	HYPH	denotes	-
T9163	15721-15722	CD	denotes	1
T9164	15732-15734	VBZ	denotes	is
T9165	15744-15747	IN	denotes	for
T9166	15748-15749	NN	denotes	S
T9167	15750-15755	NN	denotes	phase
T9168	15756-15767	NN	denotes	progression
T9169	15768-15769	-LRB-	denotes	[
T9170	15775-15777	CD	denotes	24
T9171	15769-15771	CD	denotes	16
T9172	15771-15772	,	denotes	,
T9173	15772-15774	CD	denotes	23
T9174	15774-15775	,	denotes	,
T9175	15777-15778	-RRB-	denotes	]
T9176	15778-15779	.	denotes	.
T9177	15779-16155	sentence	denotes	We show here that 3 d after transfection of the RNAi vector, p150CAF-1-depleted cells (identified by a complete absence of CAF-1 IF signal) appear still active for replication, as revealed by incorporation of the thymidine analog bromodeoxyuridine (BrdU) (Figure 3A), the PCNA (proliferating cell nuclear antigen) pattern (Figure 3B), and flow cytometry analysis (Figure 3C).
T9178	15780-15782	PRP	denotes	We
T9179	15783-15787	VBP	denotes	show
T9180	15788-15792	RB	denotes	here
T9181	15793-15797	IN	denotes	that
T9182	15920-15926	VBP	denotes	appear
T9183	15798-15799	CD	denotes	3
T9184	15800-15801	NN	denotes	d
T9185	15802-15807	IN	denotes	after
T9186	15808-15820	NN	denotes	transfection
T9187	15821-15823	IN	denotes	of
T9188	15824-15827	DT	denotes	the
T9189	15833-15839	NN	denotes	vector
T9190	15828-15832	NN	denotes	RNAi
T9191	15839-15841	,	denotes	,
T9192	15841-15848	NN	denotes	p150CAF
T9193	15851-15859	VBN	denotes	depleted
T9194	15848-15849	HYPH	denotes	-
T9195	15849-15850	CD	denotes	1
T9196	15850-15851	HYPH	denotes	-
T9197	15860-15865	NNS	denotes	cells
T9198	15866-15867	-LRB-	denotes	(
T9199	15867-15877	VBN	denotes	identified
T9200	15878-15880	IN	denotes	by
T9201	15881-15882	DT	denotes	a
T9202	15892-15899	NN	denotes	absence
T9203	15883-15891	JJ	denotes	complete
T9204	15900-15902	IN	denotes	of
T9205	15903-15906	NN	denotes	CAF
T9206	15912-15918	NN	denotes	signal
T9207	15906-15907	HYPH	denotes	-
T9208	15907-15908	CD	denotes	1
T9209	15909-15911	NN	denotes	IF
T9210	15918-15919	-RRB-	denotes	)
T9211	15927-15932	RB	denotes	still
T9212	15933-15939	JJ	denotes	active
T9213	15940-15943	IN	denotes	for
T9214	15944-15955	NN	denotes	replication
T9215	15955-15957	,	denotes	,
T9216	15957-15959	IN	denotes	as
T9217	15960-15968	VBN	denotes	revealed
T9218	15969-15971	IN	denotes	by
T9219	15972-15985	NN	denotes	incorporation
T9220	15986-15988	IN	denotes	of
T9221	15989-15992	DT	denotes	the
T9222	16003-16009	NN	denotes	analog
T9223	15993-16002	NN	denotes	thymidine
T9224	16010-16027	NN	denotes	bromodeoxyuridine
T9225	16028-16029	-LRB-	denotes	(
T9226	16029-16033	NN	denotes	BrdU
T9227	16033-16034	-RRB-	denotes	)
T9228	16035-16036	-LRB-	denotes	(
T9229	16043-16045	NN	denotes	3A
T9230	16036-16042	NN	denotes	Figure
T9231	16045-16046	-RRB-	denotes	)
T9232	16046-16048	,	denotes	,
T9233	16048-16051	DT	denotes	the
T9234	16094-16101	NN	denotes	pattern
T9235	16052-16056	NN	denotes	PCNA
T9236	16057-16058	-LRB-	denotes	(
T9237	16058-16071	VBG	denotes	proliferating
T9238	16085-16092	NN	denotes	antigen
T9239	16072-16076	NN	denotes	cell
T9240	16077-16084	JJ	denotes	nuclear
T9241	16092-16093	-RRB-	denotes	)
T9242	16102-16103	-LRB-	denotes	(
T9243	16110-16112	NN	denotes	3B
T9244	16103-16109	NN	denotes	Figure
T9245	16112-16113	-RRB-	denotes	)
T9246	16113-16115	,	denotes	,
T9247	16115-16118	CC	denotes	and
T9248	16119-16123	NN	denotes	flow
T9249	16134-16142	NN	denotes	analysis
T9250	16124-16133	NN	denotes	cytometry
T9251	16143-16144	-LRB-	denotes	(
T9252	16151-16153	NN	denotes	3C
T9253	16144-16150	NN	denotes	Figure
T9254	16153-16154	-RRB-	denotes	)
T9255	16154-16155	.	denotes	.
T9256	16155-16264	sentence	denotes	Hence, in these cells, DNA replication persists despite the drastic changes in heterochromatin organization.
T9257	16156-16161	RB	denotes	Hence
T9258	16195-16203	VBZ	denotes	persists
T9259	16161-16163	,	denotes	,
T9260	16163-16165	IN	denotes	in
T9261	16166-16171	DT	denotes	these
T9262	16172-16177	NNS	denotes	cells
T9263	16177-16179	,	denotes	,
T9264	16179-16182	NN	denotes	DNA
T9265	16183-16194	NN	denotes	replication
T9266	16204-16211	IN	denotes	despite
T9267	16212-16215	DT	denotes	the
T9268	16224-16231	NNS	denotes	changes
T9269	16216-16223	JJ	denotes	drastic
T9270	16232-16234	IN	denotes	in
T9271	16235-16250	NN	denotes	heterochromatin
T9272	16251-16263	NN	denotes	organization
T9273	16263-16264	.	denotes	.
T9274	16264-16411	sentence	denotes	However, 24 h later, ES cells cease to proliferate and die, revealing that p150CAF-1 is required for an essential cellular process, as in embryos.
T9275	16265-16272	RB	denotes	However
T9276	16295-16300	VBP	denotes	cease
T9277	16272-16274	,	denotes	,
T9278	16274-16276	CD	denotes	24
T9279	16277-16278	NN	denotes	h
T9280	16279-16284	RB	denotes	later
T9281	16284-16286	,	denotes	,
T9282	16286-16288	NN	denotes	ES
T9283	16289-16294	NNS	denotes	cells
T9284	16301-16303	TO	denotes	to
T9285	16304-16315	VB	denotes	proliferate
T9286	16316-16319	CC	denotes	and
T9287	16320-16323	VB	denotes	die
T9288	16323-16325	,	denotes	,
T9289	16325-16334	VBG	denotes	revealing
T9290	16335-16339	IN	denotes	that
T9291	16353-16361	VBN	denotes	required
T9292	16340-16347	NN	denotes	p150CAF
T9293	16347-16348	HYPH	denotes	-
T9294	16348-16349	CD	denotes	1
T9295	16350-16352	VBZ	denotes	is
T9296	16362-16365	IN	denotes	for
T9297	16366-16368	DT	denotes	an
T9298	16388-16395	NN	denotes	process
T9299	16369-16378	JJ	denotes	essential
T9300	16379-16387	JJ	denotes	cellular
T9301	16395-16397	,	denotes	,
T9302	16397-16399	IN	denotes	as
T9303	16400-16402	IN	denotes	in
T9304	16403-16410	NNS	denotes	embryos
T9305	16410-16411	.	denotes	.
T9306	16411-16623	sentence	denotes	Altogether, our data demonstrate that loss of p150CAF-1 function in ES cells and early embryos alters first the organization of heterochromatin in the nucleus and, in a subsequent step, cell cycle and viability.
T9307	16412-16422	RB	denotes	Altogether
T9308	16433-16444	VBP	denotes	demonstrate
T9309	16422-16424	,	denotes	,
T9310	16424-16427	PRP$	denotes	our
T9311	16428-16432	NNS	denotes	data
T9312	16445-16449	IN	denotes	that
T9313	16507-16513	VBZ	denotes	alters
T9314	16450-16454	NN	denotes	loss
T9315	16455-16457	IN	denotes	of
T9316	16458-16465	NN	denotes	p150CAF
T9317	16468-16476	NN	denotes	function
T9318	16465-16466	HYPH	denotes	-
T9319	16466-16467	CD	denotes	1
T9320	16477-16479	IN	denotes	in
T9321	16480-16482	NN	denotes	ES
T9322	16483-16488	NNS	denotes	cells
T9323	16489-16492	CC	denotes	and
T9324	16493-16498	JJ	denotes	early
T9325	16499-16506	NNS	denotes	embryos
T9326	16514-16519	RB	denotes	first
T9327	16520-16523	DT	denotes	the
T9328	16524-16536	NN	denotes	organization
T9329	16537-16539	IN	denotes	of
T9330	16540-16555	NN	denotes	heterochromatin
T9331	16556-16558	IN	denotes	in
T9332	16559-16562	DT	denotes	the
T9333	16563-16570	NN	denotes	nucleus
T9334	16571-16574	CC	denotes	and
T9335	16574-16576	,	denotes	,
T9336	16576-16578	IN	denotes	in
T9337	16603-16608	NN	denotes	cycle
T9338	16579-16580	DT	denotes	a
T9339	16592-16596	NN	denotes	step
T9340	16581-16591	JJ	denotes	subsequent
T9341	16596-16598	,	denotes	,
T9342	16598-16602	NN	denotes	cell
T9343	16609-16612	CC	denotes	and
T9344	16613-16622	NN	denotes	viability
T9345	16622-16623	.	denotes	.
T9346	16623-18193	sentence	denotes	Figure 3 p150CAF-1 Depletion and Loss of Heterochromatin Organization Are Compatible with Active DNA Replication in ES Cells (A) ES cells were transfected with p150CAF-1 or control siRNA vectors. After 3 d under puromycin selection, cells were pulse-labeled for 10 min with BrdU and immediately analyzed by IF. No significant difference in BrdU incorporation could be detected between control and p150CAF-1-depleted cells. The right-hand image shows the merge between the p150CAF-1 and BrdU fluorescence. Scale bar = 10 μm. (B) Immunodetection of PCNA (red) revealed no significant difference in the formation of replication foci between control and p150CAF-1-depleted ES cells. Immunodetection of p150CAF-1 is shown in green and the merging of p150CAF-1 and PCNA appears in yellow. (C) Flow cytometry analysis showed a similar cell-cycle profile for control and p150CAF-1-depleted ES cells. Results are presented as the percentage of cells in each phase of the cell cycle (G1, S, G2/M), as defined by BrdU incorporation and DNA content. Data presented are the mean of three independent experiments; error bars indicate the standard deviation. All experiments were performed 3 d after transfection of the siRNA vectors. In order to better characterize the defects in heterochromatin organization, we performed two-color DNA fluorescence in situ hybridization (FISH) experiments to reveal the spatial distribution of pericentric and centric chromosomal regions, which in mouse are mainly composed of large blocks of major and minor satellite repeats, respectively [20].
T9347	17845-17847	IN	denotes	In
T9348	17925-17934	VBD	denotes	performed
T9349	17848-17853	NN	denotes	order
T9350	17854-17856	TO	denotes	to
T9351	17864-17876	VB	denotes	characterize
T9352	17857-17863	RBR	denotes	better
T9353	17877-17880	DT	denotes	the
T9354	17881-17888	NNS	denotes	defects
T9355	17889-17891	IN	denotes	in
T9356	17892-17907	NN	denotes	heterochromatin
T9357	17908-17920	NN	denotes	organization
T9358	17920-17922	,	denotes	,
T9359	17922-17924	PRP	denotes	we
T9360	17935-17938	CD	denotes	two
T9361	17939-17944	NN	denotes	color
T9362	17938-17939	HYPH	denotes	-
T9363	17991-18002	NNS	denotes	experiments
T9364	17945-17948	NN	denotes	DNA
T9365	17949-17961	NN	denotes	fluorescence
T9366	17970-17983	NN	denotes	hybridization
T9367	17962-17964	FW	denotes	in
T9368	17965-17969	FW	denotes	situ
T9369	17984-17985	-LRB-	denotes	(
T9370	17985-17989	NN	denotes	FISH
T9371	17989-17990	-RRB-	denotes	)
T9372	18003-18005	TO	denotes	to
T9373	18006-18012	VB	denotes	reveal
T9374	18013-18016	DT	denotes	the
T9375	18025-18037	NN	denotes	distribution
T9376	18017-18024	JJ	denotes	spatial
T9377	18038-18040	IN	denotes	of
T9378	18041-18052	JJ	denotes	pericentric
T9379	18077-18084	NNS	denotes	regions
T9380	18053-18056	CC	denotes	and
T9381	18057-18064	JJ	denotes	centric
T9382	18065-18076	JJ	denotes	chromosomal
T9383	18084-18086	,	denotes	,
T9384	18086-18091	WDT	denotes	which
T9385	18112-18120	VBN	denotes	composed
T9386	18092-18094	IN	denotes	in
T9387	18095-18100	NN	denotes	mouse
T9388	18101-18104	VBP	denotes	are
T9389	18105-18111	RB	denotes	mainly
T9390	18121-18123	IN	denotes	of
T9391	18124-18129	JJ	denotes	large
T9392	18130-18136	NNS	denotes	blocks
T9393	18137-18139	IN	denotes	of
T9394	18140-18145	JJ	denotes	major
T9395	18166-18173	NNS	denotes	repeats
T9396	18146-18149	CC	denotes	and
T9397	18150-18155	JJ	denotes	minor
T9398	18156-18165	NN	denotes	satellite
T9399	18173-18175	,	denotes	,
T9400	18175-18187	RB	denotes	respectively
T9401	18188-18189	-LRB-	denotes	[
T9402	18189-18191	CD	denotes	20
T9403	18191-18192	-RRB-	denotes	]
T9404	18192-18193	.	denotes	.
T9405	18193-18371	sentence	denotes	Pericentric domains from different chromosomes form clusters, which are revealed by FISH as large spots that coincide with DAPI-dense foci in the interphase nucleus (Figure 4A).
T9406	18194-18205	JJ	denotes	Pericentric
T9407	18206-18213	NNS	denotes	domains
T9408	18241-18245	VBP	denotes	form
T9409	18214-18218	IN	denotes	from
T9410	18219-18228	JJ	denotes	different
T9411	18229-18240	NNS	denotes	chromosomes
T9412	18246-18254	NNS	denotes	clusters
T9413	18254-18256	,	denotes	,
T9414	18256-18261	WDT	denotes	which
T9415	18266-18274	VBN	denotes	revealed
T9416	18262-18265	VBP	denotes	are
T9417	18275-18277	IN	denotes	by
T9418	18278-18282	NN	denotes	FISH
T9419	18283-18285	IN	denotes	as
T9420	18286-18291	JJ	denotes	large
T9421	18292-18297	NNS	denotes	spots
T9422	18298-18302	WDT	denotes	that
T9423	18303-18311	VBP	denotes	coincide
T9424	18312-18316	IN	denotes	with
T9425	18317-18321	NN	denotes	DAPI
T9426	18322-18327	JJ	denotes	dense
T9427	18321-18322	HYPH	denotes	-
T9428	18328-18332	NNS	denotes	foci
T9429	18333-18335	IN	denotes	in
T9430	18336-18339	DT	denotes	the
T9431	18351-18358	NN	denotes	nucleus
T9432	18340-18350	NN	denotes	interphase
T9433	18359-18360	-LRB-	denotes	(
T9434	18367-18369	NN	denotes	4A
T9435	18360-18366	NN	denotes	Figure
T9436	18369-18370	-RRB-	denotes	)
T9437	18370-18371	.	denotes	.
T9438	18371-18470	sentence	denotes	At the periphery of each pericentric domain, centric regions form individual entities (Figure 4A).
T9439	18372-18374	IN	denotes	At
T9440	18433-18437	VBP	denotes	form
T9441	18375-18378	DT	denotes	the
T9442	18379-18388	NN	denotes	periphery
T9443	18389-18391	IN	denotes	of
T9444	18392-18396	DT	denotes	each
T9445	18409-18415	NN	denotes	domain
T9446	18397-18408	JJ	denotes	pericentric
T9447	18415-18417	,	denotes	,
T9448	18417-18424	JJ	denotes	centric
T9449	18425-18432	NNS	denotes	regions
T9450	18438-18448	JJ	denotes	individual
T9451	18449-18457	NNS	denotes	entities
T9452	18458-18459	-LRB-	denotes	(
T9453	18466-18468	NN	denotes	4A
T9454	18459-18465	NN	denotes	Figure
T9455	18468-18469	-RRB-	denotes	)
T9456	18469-18470	.	denotes	.
T9457	18470-18639	sentence	denotes	In p150CAF-1 RNAi-depleted cells, we observed a disruption of pericentric heterochromatin clusters that coincides with the disappearance of DAPI-dense foci (Figure 4B).
T9458	18471-18473	IN	denotes	In
T9459	18508-18516	VBD	denotes	observed
T9460	18474-18481	NN	denotes	p150CAF
T9461	18498-18503	NNS	denotes	cells
T9462	18481-18482	HYPH	denotes	-
T9463	18482-18483	CD	denotes	1
T9464	18484-18488	NN	denotes	RNAi
T9465	18489-18497	VBN	denotes	depleted
T9466	18488-18489	HYPH	denotes	-
T9467	18503-18505	,	denotes	,
T9468	18505-18507	PRP	denotes	we
T9469	18517-18518	DT	denotes	a
T9470	18519-18529	NN	denotes	disruption
T9471	18530-18532	IN	denotes	of
T9472	18533-18544	JJ	denotes	pericentric
T9473	18561-18569	NNS	denotes	clusters
T9474	18545-18560	NN	denotes	heterochromatin
T9475	18570-18574	WDT	denotes	that
T9476	18575-18584	VBZ	denotes	coincides
T9477	18585-18589	IN	denotes	with
T9478	18590-18593	DT	denotes	the
T9479	18594-18607	NN	denotes	disappearance
T9480	18608-18610	IN	denotes	of
T9481	18611-18615	NN	denotes	DAPI
T9482	18616-18621	JJ	denotes	dense
T9483	18615-18616	HYPH	denotes	-
T9484	18622-18626	NNS	denotes	foci
T9485	18627-18628	-LRB-	denotes	(
T9486	18635-18637	NN	denotes	4B
T9487	18628-18634	NN	denotes	Figure
T9488	18637-18638	-RRB-	denotes	)
T9489	18638-18639	.	denotes	.
T9490	18639-18827	sentence	denotes	Individual pericentric domains from single chromosomes are now found either isolated or aggregated in a pattern less dense than the regular clusters observed in control cells (Figure 4B).
T9491	18640-18650	JJ	denotes	Individual
T9492	18663-18670	NNS	denotes	domains
T9493	18651-18662	JJ	denotes	pericentric
T9494	18703-18708	VBN	denotes	found
T9495	18671-18675	IN	denotes	from
T9496	18676-18682	JJ	denotes	single
T9497	18683-18694	NNS	denotes	chromosomes
T9498	18695-18698	VBP	denotes	are
T9499	18699-18702	RB	denotes	now
T9500	18709-18715	CC	denotes	either
T9501	18716-18724	JJ	denotes	isolated
T9502	18725-18727	CC	denotes	or
T9503	18728-18738	JJ	denotes	aggregated
T9504	18739-18741	IN	denotes	in
T9505	18742-18743	DT	denotes	a
T9506	18744-18751	NN	denotes	pattern
T9507	18752-18756	RBR	denotes	less
T9508	18757-18762	JJ	denotes	dense
T9509	18763-18767	IN	denotes	than
T9510	18768-18771	DT	denotes	the
T9511	18780-18788	NNS	denotes	clusters
T9512	18772-18779	JJ	denotes	regular
T9513	18789-18797	VBN	denotes	observed
T9514	18798-18800	IN	denotes	in
T9515	18801-18808	NN	denotes	control
T9516	18809-18814	NNS	denotes	cells
T9517	18815-18816	-LRB-	denotes	(
T9518	18823-18825	NN	denotes	4B
T9519	18816-18822	NN	denotes	Figure
T9520	18825-18826	-RRB-	denotes	)
T9521	18826-18827	.	denotes	.
T9522	18827-18981	sentence	denotes	Such aggregates, which are often found at the nuclear periphery, are also revealed by DAPI staining as a typical signature of p150CAF-1 loss-of-function.
T9523	18828-18832	JJ	denotes	Such
T9524	18833-18843	NNS	denotes	aggregates
T9525	18902-18910	VBN	denotes	revealed
T9526	18843-18845	,	denotes	,
T9527	18845-18850	WDT	denotes	which
T9528	18861-18866	VBN	denotes	found
T9529	18851-18854	VBP	denotes	are
T9530	18855-18860	RB	denotes	often
T9531	18867-18869	IN	denotes	at
T9532	18870-18873	DT	denotes	the
T9533	18882-18891	NN	denotes	periphery
T9534	18874-18881	JJ	denotes	nuclear
T9535	18891-18893	,	denotes	,
T9536	18893-18896	VBP	denotes	are
T9537	18897-18901	RB	denotes	also
T9538	18911-18913	IN	denotes	by
T9539	18914-18918	NN	denotes	DAPI
T9540	18919-18927	NN	denotes	staining
T9541	18928-18930	IN	denotes	as
T9542	18931-18932	DT	denotes	a
T9543	18941-18950	NN	denotes	signature
T9544	18933-18940	JJ	denotes	typical
T9545	18951-18953	IN	denotes	of
T9546	18954-18961	NN	denotes	p150CAF
T9547	18961-18962	HYPH	denotes	-
T9548	18962-18963	CD	denotes	1
T9549	18964-18968	NN	denotes	loss
T9550	18968-18969	HYPH	denotes	-
T9551	18969-18971	IN	denotes	of
T9552	18971-18972	HYPH	denotes	-
T9553	18972-18980	NN	denotes	function
T9554	18980-18981	.	denotes	.
T9555	18981-19288	sentence	denotes	Quantification of fluorescence along a line randomly drawn across the nucleus revealed lower fluorescence intensity and a broader distribution of DAPI and major satellite hybridization signals in p150CAF-1-depleted cells (Figure 4C and 4D), indicating decondensation of pericentric heterochromatin domains.
T9556	18982-18996	NN	denotes	Quantification
T9557	19060-19068	VBD	denotes	revealed
T9558	18997-18999	IN	denotes	of
T9559	19000-19012	NN	denotes	fluorescence
T9560	19013-19018	IN	denotes	along
T9561	19019-19020	DT	denotes	a
T9562	19021-19025	NN	denotes	line
T9563	19026-19034	RB	denotes	randomly
T9564	19035-19040	VBN	denotes	drawn
T9565	19041-19047	IN	denotes	across
T9566	19048-19051	DT	denotes	the
T9567	19052-19059	NN	denotes	nucleus
T9568	19069-19074	JJR	denotes	lower
T9569	19088-19097	NN	denotes	intensity
T9570	19075-19087	NN	denotes	fluorescence
T9571	19098-19101	CC	denotes	and
T9572	19102-19103	DT	denotes	a
T9573	19112-19124	NN	denotes	distribution
T9574	19104-19111	JJR	denotes	broader
T9575	19125-19127	IN	denotes	of
T9576	19128-19132	NN	denotes	DAPI
T9577	19133-19136	CC	denotes	and
T9578	19137-19142	JJ	denotes	major
T9579	19167-19174	NNS	denotes	signals
T9580	19143-19152	NN	denotes	satellite
T9581	19153-19166	NN	denotes	hybridization
T9582	19175-19177	IN	denotes	in
T9583	19178-19185	NN	denotes	p150CAF
T9584	19188-19196	VBN	denotes	depleted
T9585	19185-19186	HYPH	denotes	-
T9586	19186-19187	CD	denotes	1
T9587	19187-19188	HYPH	denotes	-
T9588	19197-19202	NNS	denotes	cells
T9589	19203-19204	-LRB-	denotes	(
T9590	19211-19213	NN	denotes	4C
T9591	19204-19210	NN	denotes	Figure
T9592	19214-19217	CC	denotes	and
T9593	19218-19220	NN	denotes	4D
T9594	19220-19221	-RRB-	denotes	)
T9595	19221-19223	,	denotes	,
T9596	19223-19233	VBG	denotes	indicating
T9597	19234-19248	NN	denotes	decondensation
T9598	19249-19251	IN	denotes	of
T9599	19252-19263	JJ	denotes	pericentric
T9600	19280-19287	NNS	denotes	domains
T9601	19264-19279	NN	denotes	heterochromatin
T9602	19287-19288	.	denotes	.
T9603	19288-19455	sentence	denotes	In contrast, centric domains showed an intensity and shape similar to control cells, suggesting that they remain unaffected by p150CAF-1 depletion (Figure 4C and 4D).
T9604	19289-19291	IN	denotes	In
T9605	19318-19324	VBD	denotes	showed
T9606	19292-19300	NN	denotes	contrast
T9607	19300-19302	,	denotes	,
T9608	19302-19309	JJ	denotes	centric
T9609	19310-19317	NNS	denotes	domains
T9610	19325-19327	DT	denotes	an
T9611	19328-19337	NN	denotes	intensity
T9612	19338-19341	CC	denotes	and
T9613	19342-19347	NN	denotes	shape
T9614	19348-19355	JJ	denotes	similar
T9615	19356-19358	IN	denotes	to
T9616	19359-19366	NN	denotes	control
T9617	19367-19372	NNS	denotes	cells
T9618	19372-19374	,	denotes	,
T9619	19374-19384	VBG	denotes	suggesting
T9620	19385-19389	IN	denotes	that
T9621	19395-19401	VBP	denotes	remain
T9622	19390-19394	PRP	denotes	they
T9623	19402-19412	JJ	denotes	unaffected
T9624	19413-19415	IN	denotes	by
T9625	19416-19423	NN	denotes	p150CAF
T9626	19426-19435	NN	denotes	depletion
T9627	19423-19424	HYPH	denotes	-
T9628	19424-19425	CD	denotes	1
T9629	19436-19437	-LRB-	denotes	(
T9630	19444-19446	NN	denotes	4C
T9631	19437-19443	NN	denotes	Figure
T9632	19447-19450	CC	denotes	and
T9633	19451-19453	NN	denotes	4D
T9634	19453-19454	-RRB-	denotes	)
T9635	19454-19455	.	denotes	.
T9636	19455-19599	sentence	denotes	In conclusion, these results show that p150CAF-1 is required for the proper condensation and clustering of pericentric heterochromatin domains.
T9637	19456-19458	IN	denotes	In
T9638	19485-19489	VBP	denotes	show
T9639	19459-19469	NN	denotes	conclusion
T9640	19469-19471	,	denotes	,
T9641	19471-19476	DT	denotes	these
T9642	19477-19484	NNS	denotes	results
T9643	19490-19494	IN	denotes	that
T9644	19508-19516	VBN	denotes	required
T9645	19495-19502	NN	denotes	p150CAF
T9646	19502-19503	HYPH	denotes	-
T9647	19503-19504	CD	denotes	1
T9648	19505-19507	VBZ	denotes	is
T9649	19517-19520	IN	denotes	for
T9650	19521-19524	DT	denotes	the
T9651	19532-19544	NN	denotes	condensation
T9652	19525-19531	JJ	denotes	proper
T9653	19545-19548	CC	denotes	and
T9654	19549-19559	NN	denotes	clustering
T9655	19560-19562	IN	denotes	of
T9656	19563-19574	JJ	denotes	pericentric
T9657	19591-19598	NNS	denotes	domains
T9658	19575-19590	NN	denotes	heterochromatin
T9659	19598-19599	.	denotes	.
T9660	19599-21554	sentence	denotes	Figure 4 Depletion of p150CAF-1 Leads to Loss of Clustering, Altered Localization, and Decondensation of Pericentric Heterochromatin Domains Distribution of pericentric (red) and centric (green) domains was analyzed in the interphase nuclei of mouse ES cells by DNA FISH, using major satellite (pSAT) [47] and minor satellite (pMR150) [48] DNA probes, respectively. (A) In ES cells expressing control (cont) siRNA, pericentric regions from several chromosomes associate in clusters (red). These chromocenters form foci as revealed by DAPI staining (left-hand image), while centric regions (green) remain independent entities at the periphery of these domains. The right-hand image shows the merge between the pericentric and centric FISH signals. (B) The organization of pericentric domains was altered in cells expressing p150CAF-1 siRNA. Instead of forming well-defined chromocenters, pericentric domains were found either isolated or associated in heterogeneous aggregates of various sizes, often at the nuclear periphery. Scale bar = 10 μm. (C) Control ES cells. Fluorescence was quantified along a line randomly drawn across the nucleus in the merged image and data were plotted. One can distinguish clear peaks corresponding to chromocenters (red) and the condensed minor satellites (green). (D) ES cells expressing p150CAF-1 siRNA. p150CAF-1 depletion led to a lower fluorescence intensity and a broader distribution of signals corresponding to DAPI (blue) and major satellite hybridization (red, plot) while the organization of the minor satellites remained unaffected. Insets in the right-hand images show a typical chromocenter in control cells (C) and a disrupted chromocenter in p150CAF-1-depleted cells (D). Given the known role of CAF-1 in the deposition of histone H3.1 and H4 associated with DNA synthesis [11,12], we wondered whether this defect in higher-order chromatin organization could reflect an aberrant nucleosomal organization.
T9661	21322-21327	VBN	denotes	Given
T9662	21435-21443	VBD	denotes	wondered
T9663	21328-21331	DT	denotes	the
T9664	21338-21342	NN	denotes	role
T9665	21332-21337	VBN	denotes	known
T9666	21343-21345	IN	denotes	of
T9667	21346-21349	NN	denotes	CAF
T9668	21349-21350	HYPH	denotes	-
T9669	21350-21351	CD	denotes	1
T9670	21352-21354	IN	denotes	in
T9671	21355-21358	DT	denotes	the
T9672	21359-21369	NN	denotes	deposition
T9673	21370-21372	IN	denotes	of
T9674	21373-21380	NN	denotes	histone
T9675	21381-21385	NN	denotes	H3.1
T9676	21386-21389	CC	denotes	and
T9677	21390-21392	NN	denotes	H4
T9678	21393-21403	VBN	denotes	associated
T9679	21404-21408	IN	denotes	with
T9680	21409-21412	NN	denotes	DNA
T9681	21413-21422	NN	denotes	synthesis
T9682	21423-21424	-LRB-	denotes	[
T9683	21427-21429	CD	denotes	12
T9684	21424-21426	CD	denotes	11
T9685	21426-21427	,	denotes	,
T9686	21429-21430	-RRB-	denotes	]
T9687	21430-21432	,	denotes	,
T9688	21432-21434	PRP	denotes	we
T9689	21444-21451	IN	denotes	whether
T9690	21509-21516	VB	denotes	reflect
T9691	21452-21456	DT	denotes	this
T9692	21457-21463	NN	denotes	defect
T9693	21464-21466	IN	denotes	in
T9694	21467-21473	JJR	denotes	higher
T9695	21474-21479	NN	denotes	order
T9696	21473-21474	HYPH	denotes	-
T9697	21490-21502	NN	denotes	organization
T9698	21480-21489	NN	denotes	chromatin
T9699	21503-21508	MD	denotes	could
T9700	21517-21519	DT	denotes	an
T9701	21541-21553	NN	denotes	organization
T9702	21520-21528	JJ	denotes	aberrant
T9703	21529-21540	JJ	denotes	nucleosomal
T9704	21553-21554	.	denotes	.
T9705	21554-21781	sentence	denotes	Using DNase I and micrococcal nuclease (MNase) assays, we could not observe any significant difference between control and p150CAF-1-depleted ES cells at the level of bulk genome chromatin or at pericentric repeats (Figure 5).
T9706	21555-21560	VBG	denotes	Using
T9707	21623-21630	VB	denotes	observe
T9708	21561-21566	NN	denotes	DNase
T9709	21567-21568	CD	denotes	I
T9710	21569-21572	CC	denotes	and
T9711	21573-21584	JJ	denotes	micrococcal
T9712	21585-21593	NN	denotes	nuclease
T9713	21602-21608	NNS	denotes	assays
T9714	21594-21595	-LRB-	denotes	(
T9715	21595-21600	NN	denotes	MNase
T9716	21600-21601	-RRB-	denotes	)
T9717	21608-21610	,	denotes	,
T9718	21610-21612	PRP	denotes	we
T9719	21613-21618	MD	denotes	could
T9720	21619-21622	RB	denotes	not
T9721	21631-21634	DT	denotes	any
T9722	21647-21657	NN	denotes	difference
T9723	21635-21646	JJ	denotes	significant
T9724	21658-21665	IN	denotes	between
T9725	21666-21673	NN	denotes	control
T9726	21700-21705	NNS	denotes	cells
T9727	21674-21677	CC	denotes	and
T9728	21678-21685	NN	denotes	p150CAF
T9729	21688-21696	VBN	denotes	depleted
T9730	21685-21686	HYPH	denotes	-
T9731	21686-21687	CD	denotes	1
T9732	21687-21688	HYPH	denotes	-
T9733	21697-21699	NN	denotes	ES
T9734	21706-21708	IN	denotes	at
T9735	21709-21712	DT	denotes	the
T9736	21713-21718	NN	denotes	level
T9737	21719-21721	IN	denotes	of
T9738	21722-21726	NN	denotes	bulk
T9739	21734-21743	NN	denotes	chromatin
T9740	21727-21733	NN	denotes	genome
T9741	21744-21746	CC	denotes	or
T9742	21747-21749	IN	denotes	at
T9743	21750-21761	JJ	denotes	pericentric
T9744	21762-21769	NNS	denotes	repeats
T9745	21770-21771	-LRB-	denotes	(
T9746	21771-21777	NN	denotes	Figure
T9747	21778-21779	CD	denotes	5
T9748	21779-21780	-RRB-	denotes	)
T9749	21780-21781	.	denotes	.
T9750	21781-21964	sentence	denotes	In a second series of experiments, we compared the association of histone H3 with chromatin in nuclei isolated from cells transfected with control and p150CAF-1 RNAi plasmid vectors.
T9751	21782-21784	IN	denotes	In
T9752	21820-21828	VBD	denotes	compared
T9753	21785-21786	DT	denotes	a
T9754	21794-21800	NN	denotes	series
T9755	21787-21793	JJ	denotes	second
T9756	21801-21803	IN	denotes	of
T9757	21804-21815	NNS	denotes	experiments
T9758	21815-21817	,	denotes	,
T9759	21817-21819	PRP	denotes	we
T9760	21829-21832	DT	denotes	the
T9761	21833-21844	NN	denotes	association
T9762	21845-21847	IN	denotes	of
T9763	21848-21855	NN	denotes	histone
T9764	21856-21858	NN	denotes	H3
T9765	21859-21863	IN	denotes	with
T9766	21864-21873	NN	denotes	chromatin
T9767	21874-21876	IN	denotes	in
T9768	21877-21883	NNS	denotes	nuclei
T9769	21884-21892	VBN	denotes	isolated
T9770	21893-21897	IN	denotes	from
T9771	21898-21903	NNS	denotes	cells
T9772	21904-21915	VBN	denotes	transfected
T9773	21916-21920	IN	denotes	with
T9774	21921-21928	NN	denotes	control
T9775	21956-21963	NNS	denotes	vectors
T9776	21929-21932	CC	denotes	and
T9777	21933-21940	NN	denotes	p150CAF
T9778	21940-21941	HYPH	denotes	-
T9779	21941-21942	CD	denotes	1
T9780	21943-21947	NN	denotes	RNAi
T9781	21948-21955	NN	denotes	plasmid
T9782	21963-21964	.	denotes	.
T9783	21964-22070	sentence	denotes	Nuclei were incubated in buffers with different salt concentrations ranging from 100 mM NaCl to 1 M NaCl.
T9784	21965-21971	NNS	denotes	Nuclei
T9785	21977-21986	VBN	denotes	incubated
T9786	21972-21976	VBD	denotes	were
T9787	21987-21989	IN	denotes	in
T9788	21990-21997	NNS	denotes	buffers
T9789	21998-22002	IN	denotes	with
T9790	22003-22012	JJ	denotes	different
T9791	22018-22032	NNS	denotes	concentrations
T9792	22013-22017	NN	denotes	salt
T9793	22033-22040	VBG	denotes	ranging
T9794	22041-22045	IN	denotes	from
T9795	22046-22049	CD	denotes	100
T9796	22050-22052	NN	denotes	mM
T9797	22053-22057	NN	denotes	NaCl
T9798	22058-22060	IN	denotes	to
T9799	22061-22062	CD	denotes	1
T9800	22063-22064	NN	denotes	M
T9801	22065-22069	NN	denotes	NaCl
T9802	22069-22070	.	denotes	.
T9803	22070-22235	sentence	denotes	In all conditions, the amount of histone H3 remaining associated with chromatin was indistinguishable in control and p150CAF-1-depleted ES cells (unpublished data).
T9804	22071-22073	IN	denotes	In
T9805	22151-22154	VBD	denotes	was
T9806	22074-22077	DT	denotes	all
T9807	22078-22088	NNS	denotes	conditions
T9808	22088-22090	,	denotes	,
T9809	22090-22093	DT	denotes	the
T9810	22094-22100	NN	denotes	amount
T9811	22101-22103	IN	denotes	of
T9812	22104-22111	NN	denotes	histone
T9813	22112-22114	NN	denotes	H3
T9814	22115-22124	VBG	denotes	remaining
T9815	22125-22135	VBN	denotes	associated
T9816	22136-22140	IN	denotes	with
T9817	22141-22150	NN	denotes	chromatin
T9818	22155-22172	JJ	denotes	indistinguishable
T9819	22173-22175	IN	denotes	in
T9820	22176-22183	NN	denotes	control
T9821	22210-22215	NNS	denotes	cells
T9822	22184-22187	CC	denotes	and
T9823	22188-22195	NN	denotes	p150CAF
T9824	22198-22206	VBN	denotes	depleted
T9825	22195-22196	HYPH	denotes	-
T9826	22196-22197	CD	denotes	1
T9827	22197-22198	HYPH	denotes	-
T9828	22207-22209	NN	denotes	ES
T9829	22216-22217	-LRB-	denotes	(
T9830	22229-22233	NNS	denotes	data
T9831	22217-22228	JJ	denotes	unpublished
T9832	22233-22234	-RRB-	denotes	)
T9833	22234-22235	.	denotes	.
T9834	22235-22401	sentence	denotes	Therefore, the loss of clustering and decondensation of pericentric heterochromatin are unlikely to be the consequence of severe defects in nucleosomal organization.
T9835	22236-22245	RB	denotes	Therefore
T9836	22320-22323	VBP	denotes	are
T9837	22245-22247	,	denotes	,
T9838	22247-22250	DT	denotes	the
T9839	22251-22255	NN	denotes	loss
T9840	22256-22258	IN	denotes	of
T9841	22259-22269	NN	denotes	clustering
T9842	22270-22273	CC	denotes	and
T9843	22274-22288	NN	denotes	decondensation
T9844	22289-22291	IN	denotes	of
T9845	22292-22303	JJ	denotes	pericentric
T9846	22304-22319	NN	denotes	heterochromatin
T9847	22324-22332	JJ	denotes	unlikely
T9848	22333-22335	TO	denotes	to
T9849	22336-22338	VB	denotes	be
T9850	22339-22342	DT	denotes	the
T9851	22343-22354	NN	denotes	consequence
T9852	22355-22357	IN	denotes	of
T9853	22358-22364	JJ	denotes	severe
T9854	22365-22372	NNS	denotes	defects
T9855	22373-22375	IN	denotes	in
T9856	22376-22387	JJ	denotes	nucleosomal
T9857	22388-22400	NN	denotes	organization
T9858	22400-22401	.	denotes	.
T9859	22401-23073	sentence	denotes	Figure 5 Nucleosomal Organization Is Not Altered in p150CAF-1-Depleted ES Cells Nuclei were prepared from ES cells transfected with control or p150CAF-1 siRNA vector. Nuclei were digested with increasing amounts of DNase I or MNase. (A) After digestion with the indicated nucleases, total DNA was prepared and run onto an agarose gel which was stained with ethidium bromide to reveal bulk genomic DNA. (B) The DNA was blotted onto a nylon membrane, which was then hybridized with the α-32P-labeled pSAT major satellite repeat probe [47]. Our findings showed that the nuclear localization of HP1α is severely altered in Chaf1a −/− embryos and p150CAF-1-depleted ES cells.
T9860	22941-22944	PRP$	denotes	Our
T9861	22945-22953	NNS	denotes	findings
T9862	22954-22960	VBD	denotes	showed
T9863	22961-22965	IN	denotes	that
T9864	23011-23018	VBN	denotes	altered
T9865	22966-22969	DT	denotes	the
T9866	22978-22990	NN	denotes	localization
T9867	22970-22977	JJ	denotes	nuclear
T9868	22991-22993	IN	denotes	of
T9869	22994-22998	NN	denotes	HP1α
T9870	22999-23001	VBZ	denotes	is
T9871	23002-23010	RB	denotes	severely
T9872	23019-23021	IN	denotes	in
T9873	23022-23028	NN	denotes	Chaf1a
T9874	23033-23040	NNS	denotes	embryos
T9875	23029-23030	SYM	denotes	−
T9876	23030-23031	HYPH	denotes	/
T9877	23031-23032	SYM	denotes	−
T9878	23041-23044	CC	denotes	and
T9879	23045-23052	NN	denotes	p150CAF
T9880	23055-23063	VBN	denotes	depleted
T9881	23052-23053	HYPH	denotes	-
T9882	23053-23054	CD	denotes	1
T9883	23054-23055	HYPH	denotes	-
T9884	23067-23072	NNS	denotes	cells
T9885	23064-23066	NN	denotes	ES
T9886	23072-23073	.	denotes	.
T9887	23073-23194	sentence	denotes	We therefore examined the status of other epigenetic marks previously shown to characterize pericentric heterochromatin.
T9888	23074-23076	PRP	denotes	We
T9889	23087-23095	VBD	denotes	examined
T9890	23077-23086	RB	denotes	therefore
T9891	23096-23099	DT	denotes	the
T9892	23100-23106	NN	denotes	status
T9893	23107-23109	IN	denotes	of
T9894	23110-23115	JJ	denotes	other
T9895	23127-23132	NNS	denotes	marks
T9896	23116-23126	JJ	denotes	epigenetic
T9897	23133-23143	RB	denotes	previously
T9898	23144-23149	VBN	denotes	shown
T9899	23150-23152	TO	denotes	to
T9900	23153-23165	VB	denotes	characterize
T9901	23166-23177	JJ	denotes	pericentric
T9902	23178-23193	NN	denotes	heterochromatin
T9903	23193-23194	.	denotes	.
T9904	23194-23355	sentence	denotes	We first tested DNA CpG methylation [25] at major satellite repeats and found no significant difference between control and p150CAF-1-depleted cells (Figure 6).
T9905	23195-23197	PRP	denotes	We
T9906	23204-23210	VBD	denotes	tested
T9907	23198-23203	RB	denotes	first
T9908	23211-23214	NN	denotes	DNA
T9909	23219-23230	NN	denotes	methylation
T9910	23215-23218	NN	denotes	CpG
T9911	23231-23232	-LRB-	denotes	[
T9912	23232-23234	CD	denotes	25
T9913	23234-23235	-RRB-	denotes	]
T9914	23236-23238	IN	denotes	at
T9915	23239-23244	JJ	denotes	major
T9916	23255-23262	NNS	denotes	repeats
T9917	23245-23254	NN	denotes	satellite
T9918	23263-23266	CC	denotes	and
T9919	23267-23272	VBD	denotes	found
T9920	23273-23275	DT	denotes	no
T9921	23288-23298	NN	denotes	difference
T9922	23276-23287	JJ	denotes	significant
T9923	23299-23306	IN	denotes	between
T9924	23307-23314	NN	denotes	control
T9925	23338-23343	NNS	denotes	cells
T9926	23315-23318	CC	denotes	and
T9927	23319-23326	NN	denotes	p150CAF
T9928	23329-23337	VBN	denotes	depleted
T9929	23326-23327	HYPH	denotes	-
T9930	23327-23328	CD	denotes	1
T9931	23328-23329	HYPH	denotes	-
T9932	23344-23345	-LRB-	denotes	(
T9933	23345-23351	NN	denotes	Figure
T9934	23352-23353	CD	denotes	6
T9935	23353-23354	-RRB-	denotes	)
T9936	23354-23355	.	denotes	.
T9937	23355-23502	sentence	denotes	As the LTM7 ES cell line that we used in our RNAi experiments is a female (XX) cell line, DNA methylation is globally reduced in these cells [26].
T9938	23356-23358	IN	denotes	As
T9939	23418-23420	VBZ	denotes	is
T9940	23359-23362	DT	denotes	the
T9941	23376-23380	NN	denotes	line
T9942	23363-23367	NN	denotes	LTM7
T9943	23368-23370	NN	denotes	ES
T9944	23371-23375	NN	denotes	cell
T9945	23381-23385	WDT	denotes	that
T9946	23389-23393	VBD	denotes	used
T9947	23386-23388	PRP	denotes	we
T9948	23394-23396	IN	denotes	in
T9949	23397-23400	PRP$	denotes	our
T9950	23406-23417	NNS	denotes	experiments
T9951	23401-23405	NN	denotes	RNAi
T9952	23474-23481	VBN	denotes	reduced
T9953	23421-23422	DT	denotes	a
T9954	23440-23444	NN	denotes	line
T9955	23423-23429	JJ	denotes	female
T9956	23430-23431	-LRB-	denotes	(
T9957	23431-23433	NN	denotes	XX
T9958	23433-23434	-RRB-	denotes	)
T9959	23435-23439	NN	denotes	cell
T9960	23444-23446	,	denotes	,
T9961	23446-23449	NN	denotes	DNA
T9962	23450-23461	NN	denotes	methylation
T9963	23462-23464	VBZ	denotes	is
T9964	23465-23473	RB	denotes	globally
T9965	23482-23484	IN	denotes	in
T9966	23485-23490	DT	denotes	these
T9967	23491-23496	NNS	denotes	cells
T9968	23497-23498	-LRB-	denotes	[
T9969	23498-23500	CD	denotes	26
T9970	23500-23501	-RRB-	denotes	]
T9971	23501-23502	.	denotes	.
T9972	23502-23908	sentence	denotes	To rule out the possibility that hypomethylation of pericentric repeats might contribute to the destabilization of heterochromatin domains in p150CAF-1-depleted ES cells, we tested our RNAi plasmid vector in a XY ES cell line, and confirmed that loss of p150CAF-1 leads to disruption of heterochromatin organization independently of the degree of DNA methylation at pericentric repeats (unpublished data).
T9973	23503-23505	TO	denotes	To
T9974	23506-23510	VB	denotes	rule
T9975	23677-23683	VBD	denotes	tested
T9976	23511-23514	RP	denotes	out
T9977	23515-23518	DT	denotes	the
T9978	23519-23530	NN	denotes	possibility
T9979	23531-23535	IN	denotes	that
T9980	23581-23591	VB	denotes	contribute
T9981	23536-23551	NN	denotes	hypomethylation
T9982	23552-23554	IN	denotes	of
T9983	23555-23566	JJ	denotes	pericentric
T9984	23567-23574	NNS	denotes	repeats
T9985	23575-23580	MD	denotes	might
T9986	23592-23594	IN	denotes	to
T9987	23595-23598	DT	denotes	the
T9988	23599-23614	NN	denotes	destabilization
T9989	23615-23617	IN	denotes	of
T9990	23618-23633	NN	denotes	heterochromatin
T9991	23634-23641	NNS	denotes	domains
T9992	23642-23644	IN	denotes	in
T9993	23645-23652	NN	denotes	p150CAF
T9994	23655-23663	VBN	denotes	depleted
T9995	23652-23653	HYPH	denotes	-
T9996	23653-23654	CD	denotes	1
T9997	23654-23655	HYPH	denotes	-
T9998	23667-23672	NNS	denotes	cells
T9999	23664-23666	NN	denotes	ES
T10000	23672-23674	,	denotes	,
T10001	23674-23676	PRP	denotes	we
T10002	23684-23687	PRP$	denotes	our
T10003	23701-23707	NN	denotes	vector
T10004	23688-23692	NN	denotes	RNAi
T10005	23693-23700	NN	denotes	plasmid
T10006	23708-23710	IN	denotes	in
T10007	23711-23712	DT	denotes	a
T10008	23724-23728	NN	denotes	line
T10009	23713-23715	NN	denotes	XY
T10010	23716-23718	NN	denotes	ES
T10011	23719-23723	NN	denotes	cell
T10012	23728-23730	,	denotes	,
T10013	23730-23733	CC	denotes	and
T10014	23734-23743	VBD	denotes	confirmed
T10015	23744-23748	IN	denotes	that
T10016	23767-23772	VBZ	denotes	leads
T10017	23749-23753	NN	denotes	loss
T10018	23754-23756	IN	denotes	of
T10019	23757-23764	NN	denotes	p150CAF
T10020	23764-23765	HYPH	denotes	-
T10021	23765-23766	CD	denotes	1
T10022	23773-23775	IN	denotes	to
T10023	23776-23786	NN	denotes	disruption
T10024	23787-23789	IN	denotes	of
T10025	23790-23805	NN	denotes	heterochromatin
T10026	23806-23818	NN	denotes	organization
T10027	23819-23832	RB	denotes	independently
T10028	23833-23835	IN	denotes	of
T10029	23836-23839	DT	denotes	the
T10030	23840-23846	NN	denotes	degree
T10031	23847-23849	IN	denotes	of
T10032	23850-23853	NN	denotes	DNA
T10033	23854-23865	NN	denotes	methylation
T10034	23866-23868	IN	denotes	at
T10035	23869-23880	JJ	denotes	pericentric
T10036	23881-23888	NNS	denotes	repeats
T10037	23889-23890	-LRB-	denotes	(
T10038	23902-23906	NNS	denotes	data
T10039	23890-23901	JJ	denotes	unpublished
T10040	23906-23907	-RRB-	denotes	)
T10041	23907-23908	.	denotes	.
T10042	23908-24042	sentence	denotes	We next analyzed specific histone modifications [27] in chromatin immunoprecipitation (ChIP) experiments using native chromatin [28].
T10043	23909-23911	PRP	denotes	We
T10044	23917-23925	VBD	denotes	analyzed
T10045	23912-23916	RB	denotes	next
T10046	23926-23934	JJ	denotes	specific
T10047	23943-23956	NNS	denotes	modifications
T10048	23935-23942	NN	denotes	histone
T10049	23957-23958	-LRB-	denotes	[
T10050	23958-23960	CD	denotes	27
T10051	23960-23961	-RRB-	denotes	]
T10052	23962-23964	IN	denotes	in
T10053	23965-23974	NN	denotes	chromatin
T10054	23975-23994	NN	denotes	immunoprecipitation
T10055	24002-24013	NNS	denotes	experiments
T10056	23995-23996	-LRB-	denotes	(
T10057	23996-24000	NN	denotes	ChIP
T10058	24000-24001	-RRB-	denotes	)
T10059	24014-24019	VBG	denotes	using
T10060	24020-24026	JJ	denotes	native
T10061	24027-24036	NN	denotes	chromatin
T10062	24037-24038	-LRB-	denotes	[
T10063	24038-24040	CD	denotes	28
T10064	24040-24041	-RRB-	denotes	]
T10065	24041-24042	.	denotes	.
T10066	24042-24209	sentence	denotes	We found that pericentric DNA was immunoprecipitated with a 2-fold lower efficiency with H4K20me3 [29,30] antibodies following p150CAF-1 depletion (Figure 7A and 7B).
T10067	24043-24045	PRP	denotes	We
T10068	24046-24051	VBD	denotes	found
T10069	24052-24056	IN	denotes	that
T10070	24077-24095	VBN	denotes	immunoprecipitated
T10071	24057-24068	JJ	denotes	pericentric
T10072	24069-24072	NN	denotes	DNA
T10073	24073-24076	VBD	denotes	was
T10074	24096-24100	IN	denotes	with
T10075	24101-24102	DT	denotes	a
T10076	24116-24126	NN	denotes	efficiency
T10077	24103-24104	CD	denotes	2
T10078	24110-24115	JJR	denotes	lower
T10079	24104-24105	HYPH	denotes	-
T10080	24105-24109	RB	denotes	fold
T10081	24127-24131	IN	denotes	with
T10082	24132-24140	NN	denotes	H4K20me3
T10083	24149-24159	NNS	denotes	antibodies
T10084	24141-24142	-LRB-	denotes	[
T10085	24145-24147	CD	denotes	30
T10086	24142-24144	CD	denotes	29
T10087	24144-24145	,	denotes	,
T10088	24147-24148	-RRB-	denotes	]
T10089	24160-24169	VBG	denotes	following
T10090	24170-24177	NN	denotes	p150CAF
T10091	24180-24189	NN	denotes	depletion
T10092	24177-24178	HYPH	denotes	-
T10093	24178-24179	CD	denotes	1
T10094	24190-24191	-LRB-	denotes	(
T10095	24198-24200	NN	denotes	7A
T10096	24191-24197	NN	denotes	Figure
T10097	24201-24204	CC	denotes	and
T10098	24205-24207	NN	denotes	7B
T10099	24207-24208	-RRB-	denotes	)
T10100	24208-24209	.	denotes	.
T10101	24209-24406	sentence	denotes	Immunoprecipitation of minor satellite repeats in the same experiment was less affected (Figure 7B), showing that loss of p150CAF-1 function mainly affects H4K20me3 at pericentric heterochromatin.
T10102	24210-24229	NN	denotes	Immunoprecipitation
T10103	24289-24297	VBN	denotes	affected
T10104	24230-24232	IN	denotes	of
T10105	24233-24238	JJ	denotes	minor
T10106	24249-24256	NNS	denotes	repeats
T10107	24239-24248	NN	denotes	satellite
T10108	24257-24259	IN	denotes	in
T10109	24260-24263	DT	denotes	the
T10110	24269-24279	NN	denotes	experiment
T10111	24264-24268	JJ	denotes	same
T10112	24280-24283	VBD	denotes	was
T10113	24284-24288	RBR	denotes	less
T10114	24298-24299	-LRB-	denotes	(
T10115	24306-24308	NN	denotes	7B
T10116	24299-24305	NN	denotes	Figure
T10117	24308-24309	-RRB-	denotes	)
T10118	24309-24311	,	denotes	,
T10119	24311-24318	VBG	denotes	showing
T10120	24319-24323	IN	denotes	that
T10121	24358-24365	VBZ	denotes	affects
T10122	24324-24328	NN	denotes	loss
T10123	24329-24331	IN	denotes	of
T10124	24332-24339	NN	denotes	p150CAF
T10125	24342-24350	NN	denotes	function
T10126	24339-24340	HYPH	denotes	-
T10127	24340-24341	CD	denotes	1
T10128	24351-24357	RB	denotes	mainly
T10129	24366-24374	NN	denotes	H4K20me3
T10130	24375-24377	IN	denotes	at
T10131	24378-24389	JJ	denotes	pericentric
T10132	24390-24405	NN	denotes	heterochromatin
T10133	24405-24406	.	denotes	.
T10134	24406-24625	sentence	denotes	Moreover, no significant difference in immunoprecipitation efficiency was detected at intracisternal A particle (IAP) elements (Figure 7B), which are noncentromeric repeated DNA elements also enriched in H4K20me3 [31].
T10135	24407-24415	RB	denotes	Moreover
T10136	24481-24489	VBN	denotes	detected
T10137	24415-24417	,	denotes	,
T10138	24417-24419	DT	denotes	no
T10139	24432-24442	NN	denotes	difference
T10140	24420-24431	JJ	denotes	significant
T10141	24443-24445	IN	denotes	in
T10142	24446-24465	NN	denotes	immunoprecipitation
T10143	24466-24476	NN	denotes	efficiency
T10144	24477-24480	VBD	denotes	was
T10145	24490-24492	IN	denotes	at
T10146	24493-24507	JJ	denotes	intracisternal
T10147	24510-24518	NN	denotes	particle
T10148	24508-24509	NN	denotes	A
T10149	24525-24533	NNS	denotes	elements
T10150	24519-24520	-LRB-	denotes	(
T10151	24520-24523	NN	denotes	IAP
T10152	24523-24524	-RRB-	denotes	)
T10153	24534-24535	-LRB-	denotes	(
T10154	24542-24544	NN	denotes	7B
T10155	24535-24541	NN	denotes	Figure
T10156	24544-24545	-RRB-	denotes	)
T10157	24545-24547	,	denotes	,
T10158	24547-24552	WDT	denotes	which
T10159	24553-24556	VBP	denotes	are
T10160	24557-24571	JJ	denotes	noncentromeric
T10161	24585-24593	NNS	denotes	elements
T10162	24572-24580	VBN	denotes	repeated
T10163	24581-24584	NN	denotes	DNA
T10164	24594-24598	RB	denotes	also
T10165	24599-24607	VBN	denotes	enriched
T10166	24608-24610	IN	denotes	in
T10167	24611-24619	NN	denotes	H4K20me3
T10168	24620-24621	-LRB-	denotes	[
T10169	24621-24623	CD	denotes	31
T10170	24623-24624	-RRB-	denotes	]
T10171	24624-24625	.	denotes	.
T10172	24625-24766	sentence	denotes	This result shows that p150CAF-1 contributes to normal levels of H4K20me3 at pericentric heterochromatin, but not necessarily at other loci.
T10173	24626-24630	DT	denotes	This
T10174	24631-24637	NN	denotes	result
T10175	24638-24643	VBZ	denotes	shows
T10176	24644-24648	IN	denotes	that
T10177	24659-24670	VBZ	denotes	contributes
T10178	24649-24656	NN	denotes	p150CAF
T10179	24656-24657	HYPH	denotes	-
T10180	24657-24658	CD	denotes	1
T10181	24671-24673	IN	denotes	to
T10182	24674-24680	JJ	denotes	normal
T10183	24681-24687	NNS	denotes	levels
T10184	24688-24690	IN	denotes	of
T10185	24691-24699	NN	denotes	H4K20me3
T10186	24700-24702	IN	denotes	at
T10187	24703-24714	JJ	denotes	pericentric
T10188	24715-24730	NN	denotes	heterochromatin
T10189	24730-24732	,	denotes	,
T10190	24732-24735	CC	denotes	but
T10191	24736-24739	RB	denotes	not
T10192	24740-24751	RB	denotes	necessarily
T10193	24752-24754	IN	denotes	at
T10194	24755-24760	JJ	denotes	other
T10195	24761-24765	NNS	denotes	loci
T10196	24765-24766	.	denotes	.
T10197	24766-24908	sentence	denotes	The H3K9me3 mark [32] was also significantly reduced in p150CAF-1-depleted cells, though to a lesser extent than H4K20me3 (Figure 7A and 7B).
T10198	24767-24770	DT	denotes	The
T10199	24779-24783	NN	denotes	mark
T10200	24771-24778	NN	denotes	H3K9me3
T10201	24812-24819	VBN	denotes	reduced
T10202	24784-24785	-LRB-	denotes	[
T10203	24785-24787	CD	denotes	32
T10204	24787-24788	-RRB-	denotes	]
T10205	24789-24792	VBD	denotes	was
T10206	24793-24797	RB	denotes	also
T10207	24798-24811	RB	denotes	significantly
T10208	24820-24822	IN	denotes	in
T10209	24823-24830	NN	denotes	p150CAF
T10210	24833-24841	VBN	denotes	depleted
T10211	24830-24831	HYPH	denotes	-
T10212	24831-24832	CD	denotes	1
T10213	24832-24833	HYPH	denotes	-
T10214	24842-24847	NNS	denotes	cells
T10215	24847-24849	,	denotes	,
T10216	24849-24855	IN	denotes	though
T10217	24856-24858	IN	denotes	to
T10218	24859-24860	DT	denotes	a
T10219	24868-24874	NN	denotes	extent
T10220	24861-24867	JJR	denotes	lesser
T10221	24875-24879	IN	denotes	than
T10222	24880-24888	NN	denotes	H4K20me3
T10223	24889-24890	-LRB-	denotes	(
T10224	24897-24899	NN	denotes	7A
T10225	24890-24896	NN	denotes	Figure
T10226	24900-24903	CC	denotes	and
T10227	24904-24906	NN	denotes	7B
T10228	24906-24907	-RRB-	denotes	)
T10229	24907-24908	.	denotes	.
T10230	24908-26806	sentence	denotes	Figure 6 DNA CpG Methylation at Pericentric Heterochromatin Is Not Altered in p150CAF-1-Depleted ES Cells Total DNA was isolated from cells transfected with a control or the p150CAF-1 siRNA vector and digested with MaeII, whose recognition sequence is present in major satellite repeats. Digested DNA was run onto an agarose gel and analyzed by Southern blotting using the pSAT major satellite probe [47]. Figure 7 Alteration of Epigenetic Marking at Pericentric Heterochromatin in p150CAF-1-Depleted Cells (A) p150CAF-1 depletion leads to reduced H4K20me3 and H3K9me3 at pericentric heterochromatin. Enrichment of histone marks at major satellite repeats was determined by ChIP from control (cont) and p150CAF-1 (p150) siRNA-expressing ES cells. DNA prepared from the input and the antibody-bound fraction were run onto an agarose gel and analyzed by Southern blot with the pSAT major satellite repeat probe [47]. (B) Hybridization signals were quantified using an Instant Imager. After autoradiography, the membrane was stripped and rehybridized with a minor satellite probe [49]. After quantification, the membrane was stripped and rehybridized with an IAP LTR probe [50]. Results are presented as the amount of DNA immunoprecipitated from p150CAF-1-depleted ES cells divided by the DNA obtained from control cells. The figure shows the mean value and standard deviation of three independent ChIP experiments. (C and D) H3K9me3 and H4K20me3 fluorescence patterns are severely altered in p150CAF-1-depleted ES cells. Immunodetection of H3K9me3 (C, green), H4K20me3 (D, green), and HP1α (red) in control and p150CAF-1 siRNA-expressing ES cells. Merging of HP1α with H3K9me3 (C) and H4K20me3 (D) is shown in yellow. Scale bars represent 10 μm. In wild-type cells, most of H4K20me3 and H3K9me3 signals are present at the level of DAPI-dense domains when visualized by IF [29] (Figures 7C and 7D).
T10231	26655-26657	IN	denotes	In
T10232	26712-26715	VBP	denotes	are
T10233	26658-26662	JJ	denotes	wild
T10234	26663-26667	NN	denotes	type
T10235	26662-26663	HYPH	denotes	-
T10236	26668-26673	NNS	denotes	cells
T10237	26673-26675	,	denotes	,
T10238	26675-26679	JJS	denotes	most
T10239	26680-26682	IN	denotes	of
T10240	26683-26691	NN	denotes	H4K20me3
T10241	26704-26711	NNS	denotes	signals
T10242	26692-26695	CC	denotes	and
T10243	26696-26703	NN	denotes	H3K9me3
T10244	26716-26723	JJ	denotes	present
T10245	26724-26726	IN	denotes	at
T10246	26727-26730	DT	denotes	the
T10247	26731-26736	NN	denotes	level
T10248	26737-26739	IN	denotes	of
T10249	26740-26744	NN	denotes	DAPI
T10250	26745-26750	JJ	denotes	dense
T10251	26744-26745	HYPH	denotes	-
T10252	26751-26758	NNS	denotes	domains
T10253	26759-26763	WRB	denotes	when
T10254	26764-26774	VBN	denotes	visualized
T10255	26775-26777	IN	denotes	by
T10256	26778-26780	NN	denotes	IF
T10257	26781-26782	-LRB-	denotes	[
T10258	26782-26784	CD	denotes	29
T10259	26784-26785	-RRB-	denotes	]
T10260	26786-26787	-LRB-	denotes	(
T10261	26795-26797	NN	denotes	7C
T10262	26787-26794	NNS	denotes	Figures
T10263	26798-26801	CC	denotes	and
T10264	26802-26804	NN	denotes	7D
T10265	26804-26805	-RRB-	denotes	)
T10266	26805-26806	.	denotes	.
T10267	26806-26924	sentence	denotes	H3K9me3 was severely perturbed in p150CAF-1-depleted cells, displaying a diffuse pattern similar to HP1α (Figure 7C).
T10268	26807-26814	NN	denotes	H3K9me3
T10269	26828-26837	VBN	denotes	perturbed
T10270	26815-26818	VBD	denotes	was
T10271	26819-26827	RB	denotes	severely
T10272	26838-26840	IN	denotes	in
T10273	26841-26848	NN	denotes	p150CAF
T10274	26851-26859	VBN	denotes	depleted
T10275	26848-26849	HYPH	denotes	-
T10276	26849-26850	CD	denotes	1
T10277	26850-26851	HYPH	denotes	-
T10278	26860-26865	NNS	denotes	cells
T10279	26865-26867	,	denotes	,
T10280	26867-26877	VBG	denotes	displaying
T10281	26878-26879	DT	denotes	a
T10282	26888-26895	NN	denotes	pattern
T10283	26880-26887	JJ	denotes	diffuse
T10284	26896-26903	JJ	denotes	similar
T10285	26904-26906	IN	denotes	to
T10286	26907-26911	NN	denotes	HP1α
T10287	26912-26913	-LRB-	denotes	(
T10288	26920-26922	NN	denotes	7C
T10289	26913-26919	NN	denotes	Figure
T10290	26922-26923	-RRB-	denotes	)
T10291	26923-26924	.	denotes	.
T10292	26924-27092	sentence	denotes	The H4K20me3 pattern was even more severely altered, showing both a loss of the typical dots revealed in control cells and a reduction in signal intensity (Figure 7D).
T10293	26925-26928	DT	denotes	The
T10294	26938-26945	NN	denotes	pattern
T10295	26929-26937	NN	denotes	H4K20me3
T10296	26969-26976	VBN	denotes	altered
T10297	26946-26949	VBD	denotes	was
T10298	26950-26954	RB	denotes	even
T10299	26955-26959	RBR	denotes	more
T10300	26960-26968	RB	denotes	severely
T10301	26976-26978	,	denotes	,
T10302	26978-26985	VBG	denotes	showing
T10303	26986-26990	CC	denotes	both
T10304	26993-26997	NN	denotes	loss
T10305	26991-26992	DT	denotes	a
T10306	26998-27000	IN	denotes	of
T10307	27001-27004	DT	denotes	the
T10308	27013-27017	NNS	denotes	dots
T10309	27005-27012	JJ	denotes	typical
T10310	27018-27026	VBN	denotes	revealed
T10311	27027-27029	IN	denotes	in
T10312	27030-27037	NN	denotes	control
T10313	27038-27043	NNS	denotes	cells
T10314	27044-27047	CC	denotes	and
T10315	27048-27049	DT	denotes	a
T10316	27050-27059	NN	denotes	reduction
T10317	27060-27062	IN	denotes	in
T10318	27063-27069	NN	denotes	signal
T10319	27070-27079	NN	denotes	intensity
T10320	27080-27081	-LRB-	denotes	(
T10321	27088-27090	NN	denotes	7D
T10322	27081-27087	NN	denotes	Figure
T10323	27090-27091	-RRB-	denotes	)
T10324	27091-27092	.	denotes	.
T10325	27092-27218	sentence	denotes	Consistent with our ChIP experiments, residual H4K20me3 epitopes were detected at the level of relocated DAPI-dense material.
T10326	27093-27103	JJ	denotes	Consistent
T10327	27163-27171	VBN	denotes	detected
T10328	27104-27108	IN	denotes	with
T10329	27109-27112	PRP$	denotes	our
T10330	27118-27129	NNS	denotes	experiments
T10331	27113-27117	NN	denotes	ChIP
T10332	27129-27131	,	denotes	,
T10333	27131-27139	JJ	denotes	residual
T10334	27149-27157	NNS	denotes	epitopes
T10335	27140-27148	NN	denotes	H4K20me3
T10336	27158-27162	VBD	denotes	were
T10337	27172-27174	IN	denotes	at
T10338	27175-27178	DT	denotes	the
T10339	27179-27184	NN	denotes	level
T10340	27185-27187	IN	denotes	of
T10341	27188-27197	VBN	denotes	relocated
T10342	27209-27217	NN	denotes	material
T10343	27198-27202	NN	denotes	DAPI
T10344	27203-27208	JJ	denotes	dense
T10345	27202-27203	HYPH	denotes	-
T10346	27217-27218	.	denotes	.
T10347	27218-27395	sentence	denotes	Altogether, our results show that depletion of CAF-1 in ES cells leads to a simultaneous disruption of heterochromatin 3-D organization and an alteration in epigenetic marking.
T10348	27219-27229	RB	denotes	Altogether
T10349	27243-27247	VBP	denotes	show
T10350	27229-27231	,	denotes	,
T10351	27231-27234	PRP$	denotes	our
T10352	27235-27242	NNS	denotes	results
T10353	27248-27252	IN	denotes	that
T10354	27284-27289	VBZ	denotes	leads
T10355	27253-27262	NN	denotes	depletion
T10356	27263-27265	IN	denotes	of
T10357	27266-27269	NN	denotes	CAF
T10358	27269-27270	HYPH	denotes	-
T10359	27270-27271	CD	denotes	1
T10360	27272-27274	IN	denotes	in
T10361	27275-27277	NN	denotes	ES
T10362	27278-27283	NNS	denotes	cells
T10363	27290-27292	IN	denotes	to
T10364	27293-27294	DT	denotes	a
T10365	27308-27318	NN	denotes	disruption
T10366	27295-27307	JJ	denotes	simultaneous
T10367	27319-27321	IN	denotes	of
T10368	27322-27337	NN	denotes	heterochromatin
T10369	27342-27354	NN	denotes	organization
T10370	27338-27339	CD	denotes	3
T10371	27340-27341	NN	denotes	D
T10372	27339-27340	HYPH	denotes	-
T10373	27355-27358	CC	denotes	and
T10374	27359-27361	DT	denotes	an
T10375	27362-27372	NN	denotes	alteration
T10376	27373-27375	IN	denotes	in
T10377	27376-27386	JJ	denotes	epigenetic
T10378	27387-27394	NN	denotes	marking
T10379	27394-27395	.	denotes	.
T13122	27408-27412	RB	denotes	Here
T13123	27417-27424	VBP	denotes	provide
T13124	27412-27414	,	denotes	,
T13125	27414-27416	PRP	denotes	we
T13126	27425-27433	NN	denotes	evidence
T13127	27434-27438	IN	denotes	that
T13128	27449-27452	VBZ	denotes	has
T13129	27439-27446	NN	denotes	p150CAF
T13130	27446-27447	HYPH	denotes	-
T13131	27447-27448	CD	denotes	1
T13132	27453-27454	DT	denotes	a
T13133	27463-27471	NN	denotes	function
T13134	27455-27462	JJ	denotes	crucial
T13135	27472-27478	IN	denotes	during
T13136	27479-27484	NN	denotes	mouse
T13137	27491-27504	NN	denotes	embryogenesis
T13138	27485-27490	JJ	denotes	early
T13139	27505-27508	CC	denotes	and
T13140	27509-27511	IN	denotes	in
T13141	27512-27514	NN	denotes	ES
T13142	27515-27520	NNS	denotes	cells
T13143	27520-27521	.	denotes	.
T13144	27521-27624	sentence	denotes	Previous studies showed that CAF-1 is essential for the progression of DNA replication in vertebrates.
T13145	27522-27530	JJ	denotes	Previous
T13146	27531-27538	NNS	denotes	studies
T13147	27539-27545	VBD	denotes	showed
T13148	27546-27550	IN	denotes	that
T13149	27557-27559	VBZ	denotes	is
T13150	27551-27554	NN	denotes	CAF
T13151	27554-27555	HYPH	denotes	-
T13152	27555-27556	CD	denotes	1
T13153	27560-27569	JJ	denotes	essential
T13154	27570-27573	IN	denotes	for
T13155	27574-27577	DT	denotes	the
T13156	27578-27589	NN	denotes	progression
T13157	27590-27592	IN	denotes	of
T13158	27593-27596	NN	denotes	DNA
T13159	27597-27608	NN	denotes	replication
T13160	27609-27611	IN	denotes	in
T13161	27612-27623	NNS	denotes	vertebrates
T13162	27623-27624	.	denotes	.
T13163	27624-27745	sentence	denotes	In Xenopus laevis, p150CAF-1 function is required for the rapid cell divisions that occur during early development [33].
T13164	27625-27627	IN	denotes	In
T13165	27666-27674	VBN	denotes	required
T13166	27628-27635	NNP	denotes	Xenopus
T13167	27636-27642	NNP	denotes	laevis
T13168	27642-27644	,	denotes	,
T13169	27644-27651	NN	denotes	p150CAF
T13170	27654-27662	NN	denotes	function
T13171	27651-27652	HYPH	denotes	-
T13172	27652-27653	CD	denotes	1
T13173	27663-27665	VBZ	denotes	is
T13174	27675-27678	IN	denotes	for
T13175	27679-27682	DT	denotes	the
T13176	27694-27703	NNS	denotes	divisions
T13177	27683-27688	JJ	denotes	rapid
T13178	27689-27693	NN	denotes	cell
T13179	27704-27708	WDT	denotes	that
T13180	27709-27714	VBP	denotes	occur
T13181	27715-27721	IN	denotes	during
T13182	27722-27727	JJ	denotes	early
T13183	27728-27739	NN	denotes	development
T13184	27740-27741	-LRB-	denotes	[
T13185	27741-27743	CD	denotes	33
T13186	27743-27744	-RRB-	denotes	]
T13187	27744-27745	.	denotes	.
T13188	27745-27853	sentence	denotes	Similarly, CAF-1 is essential in mammalian cell lines for the progression of DNA replication [16,23,24,34].
T13189	27746-27755	RB	denotes	Similarly
T13190	27763-27765	VBZ	denotes	is
T13191	27755-27757	,	denotes	,
T13192	27757-27760	NN	denotes	CAF
T13193	27760-27761	HYPH	denotes	-
T13194	27761-27762	CD	denotes	1
T13195	27766-27775	JJ	denotes	essential
T13196	27776-27778	IN	denotes	in
T13197	27779-27788	JJ	denotes	mammalian
T13198	27794-27799	NNS	denotes	lines
T13199	27789-27793	NN	denotes	cell
T13200	27800-27803	IN	denotes	for
T13201	27804-27807	DT	denotes	the
T13202	27808-27819	NN	denotes	progression
T13203	27820-27822	IN	denotes	of
T13204	27823-27826	NN	denotes	DNA
T13205	27827-27838	NN	denotes	replication
T13206	27839-27840	-LRB-	denotes	[
T13207	27849-27851	CD	denotes	34
T13208	27840-27842	CD	denotes	16
T13209	27842-27843	,	denotes	,
T13210	27843-27845	CD	denotes	23
T13211	27845-27846	,	denotes	,
T13212	27846-27848	CD	denotes	24
T13213	27848-27849	,	denotes	,
T13214	27851-27852	-RRB-	denotes	]
T13215	27852-27853	.	denotes	.
T13216	27853-27987	sentence	denotes	Our findings reveal a role for CAF-1 in the nuclear organization of heterochromatin domains during early development and in ES cells.
T13217	27854-27857	PRP$	denotes	Our
T13218	27858-27866	NNS	denotes	findings
T13219	27867-27873	VBP	denotes	reveal
T13220	27874-27875	DT	denotes	a
T13221	27876-27880	NN	denotes	role
T13222	27881-27884	IN	denotes	for
T13223	27885-27888	NN	denotes	CAF
T13224	27888-27889	HYPH	denotes	-
T13225	27889-27890	CD	denotes	1
T13226	27891-27893	IN	denotes	in
T13227	27894-27897	DT	denotes	the
T13228	27906-27918	NN	denotes	organization
T13229	27898-27905	JJ	denotes	nuclear
T13230	27919-27921	IN	denotes	of
T13231	27922-27937	NN	denotes	heterochromatin
T13232	27938-27945	NNS	denotes	domains
T13233	27946-27952	IN	denotes	during
T13234	27953-27958	JJ	denotes	early
T13235	27959-27970	NN	denotes	development
T13236	27971-27974	CC	denotes	and
T13237	27975-27977	IN	denotes	in
T13238	27978-27980	NN	denotes	ES
T13239	27981-27986	NNS	denotes	cells
T13240	27986-27987	.	denotes	.
T13241	27987-28196	sentence	denotes	We show that in p150CAF-1-depleted ES cells, while DNA replication initially persists without significant perturbation in nucleosomal organization, severe heterochromatin organization defects can be observed.
T13242	27988-27990	PRP	denotes	We
T13243	27991-27995	VBP	denotes	show
T13244	27996-28000	IN	denotes	that
T13245	28187-28195	VBN	denotes	observed
T13246	28001-28003	IN	denotes	in
T13247	28004-28011	NN	denotes	p150CAF
T13248	28014-28022	VBN	denotes	depleted
T13249	28011-28012	HYPH	denotes	-
T13250	28012-28013	CD	denotes	1
T13251	28013-28014	HYPH	denotes	-
T13252	28026-28031	NNS	denotes	cells
T13253	28023-28025	NN	denotes	ES
T13254	28031-28033	,	denotes	,
T13255	28033-28038	IN	denotes	while
T13256	28065-28073	VBZ	denotes	persists
T13257	28039-28042	NN	denotes	DNA
T13258	28043-28054	NN	denotes	replication
T13259	28055-28064	RB	denotes	initially
T13260	28074-28081	IN	denotes	without
T13261	28082-28093	JJ	denotes	significant
T13262	28094-28106	NN	denotes	perturbation
T13263	28107-28109	IN	denotes	in
T13264	28110-28121	JJ	denotes	nucleosomal
T13265	28122-28134	NN	denotes	organization
T13266	28134-28136	,	denotes	,
T13267	28136-28142	JJ	denotes	severe
T13268	28172-28179	NNS	denotes	defects
T13269	28143-28158	NN	denotes	heterochromatin
T13270	28159-28171	NN	denotes	organization
T13271	28180-28183	MD	denotes	can
T13272	28184-28186	VB	denotes	be
T13273	28195-28196	.	denotes	.
T13274	28196-28319	sentence	denotes	Loss of p150CAF-1 did not affect PML bodies in ES cell nuclei, showing that nuclear architecture is not generally altered.
T13275	28197-28201	NN	denotes	Loss
T13276	28223-28229	VB	denotes	affect
T13277	28202-28204	IN	denotes	of
T13278	28205-28212	NN	denotes	p150CAF
T13279	28212-28213	HYPH	denotes	-
T13280	28213-28214	CD	denotes	1
T13281	28215-28218	VBD	denotes	did
T13282	28219-28222	RB	denotes	not
T13283	28230-28233	NN	denotes	PML
T13284	28234-28240	NNS	denotes	bodies
T13285	28241-28243	IN	denotes	in
T13286	28244-28246	NN	denotes	ES
T13287	28252-28258	NNS	denotes	nuclei
T13288	28247-28251	NN	denotes	cell
T13289	28258-28260	,	denotes	,
T13290	28260-28267	VBG	denotes	showing
T13291	28268-28272	IN	denotes	that
T13292	28311-28318	VBN	denotes	altered
T13293	28273-28280	JJ	denotes	nuclear
T13294	28281-28293	NN	denotes	architecture
T13295	28294-28296	VBZ	denotes	is
T13296	28297-28300	RB	denotes	not
T13297	28301-28310	RB	denotes	generally
T13298	28318-28319	.	denotes	.
T13299	28319-28409	sentence	denotes	Ultimately, p150CAF-1 depletion results in the arrest of ES cell division and cell death.
T13300	28320-28330	RB	denotes	Ultimately
T13301	28352-28359	VBZ	denotes	results
T13302	28330-28332	,	denotes	,
T13303	28332-28339	NN	denotes	p150CAF
T13304	28342-28351	NN	denotes	depletion
T13305	28339-28340	HYPH	denotes	-
T13306	28340-28341	CD	denotes	1
T13307	28360-28362	IN	denotes	in
T13308	28363-28366	DT	denotes	the
T13309	28367-28373	NN	denotes	arrest
T13310	28374-28376	IN	denotes	of
T13311	28377-28379	NN	denotes	ES
T13312	28380-28384	NN	denotes	cell
T13313	28385-28393	NN	denotes	division
T13314	28394-28397	CC	denotes	and
T13315	28398-28402	NN	denotes	cell
T13316	28403-28408	NN	denotes	death
T13317	28408-28409	.	denotes	.
T13318	28409-28591	sentence	denotes	Given the major defects observed in heterochromatin organization, it is tempting to speculate that these defects cause the developmental arrest in the embryos and death in ES cells.
T13319	28410-28415	VBN	denotes	Given
T13320	28479-28481	VBZ	denotes	is
T13321	28416-28419	DT	denotes	the
T13322	28426-28433	NNS	denotes	defects
T13323	28420-28425	JJ	denotes	major
T13324	28434-28442	VBN	denotes	observed
T13325	28443-28445	IN	denotes	in
T13326	28446-28461	NN	denotes	heterochromatin
T13327	28462-28474	NN	denotes	organization
T13328	28474-28476	,	denotes	,
T13329	28476-28478	PRP	denotes	it
T13330	28482-28490	VBG	denotes	tempting
T13331	28491-28493	TO	denotes	to
T13332	28494-28503	VB	denotes	speculate
T13333	28504-28508	IN	denotes	that
T13334	28523-28528	VBP	denotes	cause
T13335	28509-28514	DT	denotes	these
T13336	28515-28522	NNS	denotes	defects
T13337	28529-28532	DT	denotes	the
T13338	28547-28553	NN	denotes	arrest
T13339	28533-28546	JJ	denotes	developmental
T13340	28554-28556	IN	denotes	in
T13341	28557-28560	DT	denotes	the
T13342	28561-28568	NNS	denotes	embryos
T13343	28569-28572	CC	denotes	and
T13344	28573-28578	NN	denotes	death
T13345	28579-28581	IN	denotes	in
T13346	28582-28584	NN	denotes	ES
T13347	28585-28590	NNS	denotes	cells
T13348	28590-28591	.	denotes	.
T13349	28591-28740	sentence	denotes	The role of CAF-1 in the nuclear organization of heterochromatin includes spatial localization, condensation, and clustering of pericentric domains.
T13350	28592-28595	DT	denotes	The
T13351	28596-28600	NN	denotes	role
T13352	28657-28665	VBZ	denotes	includes
T13353	28601-28603	IN	denotes	of
T13354	28604-28607	NN	denotes	CAF
T13355	28607-28608	HYPH	denotes	-
T13356	28608-28609	CD	denotes	1
T13357	28610-28612	IN	denotes	in
T13358	28613-28616	DT	denotes	the
T13359	28625-28637	NN	denotes	organization
T13360	28617-28624	JJ	denotes	nuclear
T13361	28638-28640	IN	denotes	of
T13362	28641-28656	NN	denotes	heterochromatin
T13363	28666-28673	JJ	denotes	spatial
T13364	28674-28686	NN	denotes	localization
T13365	28686-28688	,	denotes	,
T13366	28688-28700	NN	denotes	condensation
T13367	28700-28702	,	denotes	,
T13368	28702-28705	CC	denotes	and
T13369	28706-28716	NN	denotes	clustering
T13370	28717-28719	IN	denotes	of
T13371	28720-28731	JJ	denotes	pericentric
T13372	28732-28739	NNS	denotes	domains
T13373	28739-28740	.	denotes	.
T13374	28740-28902	sentence	denotes	This important function of CAF-1 was not revealed in primary MEFs, 3T3 cells, or human cell lines, suggesting that it is specific of pluripotent embryonic cells.
T13375	28741-28745	DT	denotes	This
T13376	28756-28764	NN	denotes	function
T13377	28746-28755	JJ	denotes	important
T13378	28782-28790	VBN	denotes	revealed
T13379	28765-28767	IN	denotes	of
T13380	28768-28771	NN	denotes	CAF
T13381	28771-28772	HYPH	denotes	-
T13382	28772-28773	CD	denotes	1
T13383	28774-28777	VBD	denotes	was
T13384	28778-28781	RB	denotes	not
T13385	28791-28793	IN	denotes	in
T13386	28794-28801	JJ	denotes	primary
T13387	28802-28806	NNS	denotes	MEFs
T13388	28806-28808	,	denotes	,
T13389	28808-28811	NN	denotes	3T3
T13390	28812-28817	NNS	denotes	cells
T13391	28817-28819	,	denotes	,
T13392	28819-28821	CC	denotes	or
T13393	28822-28827	JJ	denotes	human
T13394	28833-28838	NNS	denotes	lines
T13395	28828-28832	NN	denotes	cell
T13396	28838-28840	,	denotes	,
T13397	28840-28850	VBG	denotes	suggesting
T13398	28851-28855	IN	denotes	that
T13399	28859-28861	VBZ	denotes	is
T13400	28856-28858	PRP	denotes	it
T13401	28862-28870	JJ	denotes	specific
T13402	28871-28873	IN	denotes	of
T13403	28874-28885	JJ	denotes	pluripotent
T13404	28896-28901	NNS	denotes	cells
T13405	28886-28895	JJ	denotes	embryonic
T13406	28901-28902	.	denotes	.
T13407	28902-29124	sentence	denotes	Alternatively, early embryos and ES cells, whose genome is more plastic than somatic cells, might represent a particularly sensitive context in which the importance of CAF-1 in heterochromatin organization is exacerbated.
T13408	28903-28916	RB	denotes	Alternatively
T13409	29001-29010	VB	denotes	represent
T13410	28916-28918	,	denotes	,
T13411	28918-28923	JJ	denotes	early
T13412	28924-28931	NNS	denotes	embryos
T13413	28932-28935	CC	denotes	and
T13414	28936-28938	NN	denotes	ES
T13415	28939-28944	NNS	denotes	cells
T13416	28944-28946	,	denotes	,
T13417	28946-28951	WP$	denotes	whose
T13418	28952-28958	NN	denotes	genome
T13419	28959-28961	VBZ	denotes	is
T13420	28962-28966	RBR	denotes	more
T13421	28967-28974	JJ	denotes	plastic
T13422	28975-28979	IN	denotes	than
T13423	28980-28987	JJ	denotes	somatic
T13424	28988-28993	NNS	denotes	cells
T13425	28993-28995	,	denotes	,
T13426	28995-29000	MD	denotes	might
T13427	29011-29012	DT	denotes	a
T13428	29036-29043	NN	denotes	context
T13429	29013-29025	RB	denotes	particularly
T13430	29026-29035	JJ	denotes	sensitive
T13431	29044-29046	IN	denotes	in
T13432	29112-29123	VBN	denotes	exacerbated
T13433	29047-29052	WDT	denotes	which
T13434	29053-29056	DT	denotes	the
T13435	29057-29067	NN	denotes	importance
T13436	29068-29070	IN	denotes	of
T13437	29071-29074	NN	denotes	CAF
T13438	29074-29075	HYPH	denotes	-
T13439	29075-29076	CD	denotes	1
T13440	29077-29079	IN	denotes	in
T13441	29080-29095	NN	denotes	heterochromatin
T13442	29096-29108	NN	denotes	organization
T13443	29109-29111	VBZ	denotes	is
T13444	29123-29124	.	denotes	.
T13445	29124-29240	sentence	denotes	In ES cells, major architectural chromatin proteins are hyperdynamic and bind relatively loosely to chromatin [35].
T13446	29125-29127	IN	denotes	In
T13447	29177-29180	VBP	denotes	are
T13448	29128-29130	NN	denotes	ES
T13449	29131-29136	NNS	denotes	cells
T13450	29136-29138	,	denotes	,
T13451	29138-29143	JJ	denotes	major
T13452	29168-29176	NN	denotes	proteins
T13453	29144-29157	JJ	denotes	architectural
T13454	29158-29167	NN	denotes	chromatin
T13455	29181-29193	JJ	denotes	hyperdynamic
T13456	29194-29197	CC	denotes	and
T13457	29198-29202	VBP	denotes	bind
T13458	29203-29213	RB	denotes	relatively
T13459	29214-29221	RB	denotes	loosely
T13460	29222-29224	IN	denotes	to
T13461	29225-29234	NN	denotes	chromatin
T13462	29235-29236	-LRB-	denotes	[
T13463	29236-29238	CD	denotes	35
T13464	29238-29239	-RRB-	denotes	]
T13465	29239-29240	.	denotes	.
T13466	29240-29359	sentence	denotes	In this cellular context, our findings show that proper heterochromatin nuclear architecture relies on CAF-1 function.
T13467	29241-29243	IN	denotes	In
T13468	29280-29284	VBP	denotes	show
T13469	29244-29248	DT	denotes	this
T13470	29258-29265	NN	denotes	context
T13471	29249-29257	JJ	denotes	cellular
T13472	29265-29267	,	denotes	,
T13473	29267-29270	PRP$	denotes	our
T13474	29271-29279	NNS	denotes	findings
T13475	29285-29289	IN	denotes	that
T13476	29334-29340	VBZ	denotes	relies
T13477	29290-29296	JJ	denotes	proper
T13478	29321-29333	NN	denotes	architecture
T13479	29297-29312	NN	denotes	heterochromatin
T13480	29313-29320	JJ	denotes	nuclear
T13481	29341-29343	IN	denotes	on
T13482	29344-29347	NN	denotes	CAF
T13483	29350-29358	NN	denotes	function
T13484	29347-29348	HYPH	denotes	-
T13485	29348-29349	CD	denotes	1
T13486	29358-29359	.	denotes	.
T13487	29359-29555	sentence	denotes	In contrast, in undifferentiated cells that are already lineage committed (such as MEFs) genome architecture might be more stable than in ES cells and thus cannot be disrupted by CAF-1 depletion.
T13488	29360-29362	IN	denotes	In
T13489	29475-29477	VB	denotes	be
T13490	29363-29371	NN	denotes	contrast
T13491	29371-29373	,	denotes	,
T13492	29373-29375	IN	denotes	in
T13493	29376-29392	JJ	denotes	undifferentiated
T13494	29393-29398	NNS	denotes	cells
T13495	29399-29403	WDT	denotes	that
T13496	29424-29433	VBN	denotes	committed
T13497	29404-29407	VBP	denotes	are
T13498	29408-29415	RB	denotes	already
T13499	29416-29423	NN	denotes	lineage
T13500	29434-29435	-LRB-	denotes	(
T13501	29435-29439	JJ	denotes	such
T13502	29440-29442	IN	denotes	as
T13503	29443-29447	NNS	denotes	MEFs
T13504	29447-29448	-RRB-	denotes	)
T13505	29449-29455	NN	denotes	genome
T13506	29456-29468	NN	denotes	architecture
T13507	29469-29474	MD	denotes	might
T13508	29478-29482	RBR	denotes	more
T13509	29483-29489	JJ	denotes	stable
T13510	29490-29494	IN	denotes	than
T13511	29495-29497	IN	denotes	in
T13512	29498-29500	NN	denotes	ES
T13513	29501-29506	NNS	denotes	cells
T13514	29507-29510	CC	denotes	and
T13515	29511-29515	RB	denotes	thus
T13516	29526-29535	VBN	denotes	disrupted
T13517	29516-29519	MD	denotes	can
T13518	29519-29522	RB	denotes	not
T13519	29523-29525	VB	denotes	be
T13520	29536-29538	IN	denotes	by
T13521	29539-29542	NN	denotes	CAF
T13522	29545-29554	NN	denotes	depletion
T13523	29542-29543	HYPH	denotes	-
T13524	29543-29544	CD	denotes	1
T13525	29554-29555	.	denotes	.
T13526	29555-29666	sentence	denotes	This important function of CAF-1 in pluripotent embryonic cells could be partially conserved during evolution.
T13527	29556-29560	DT	denotes	This
T13528	29571-29579	NN	denotes	function
T13529	29561-29570	JJ	denotes	important
T13530	29639-29648	VBN	denotes	conserved
T13531	29580-29582	IN	denotes	of
T13532	29583-29586	NN	denotes	CAF
T13533	29586-29587	HYPH	denotes	-
T13534	29587-29588	CD	denotes	1
T13535	29589-29591	IN	denotes	in
T13536	29592-29603	JJ	denotes	pluripotent
T13537	29614-29619	NNS	denotes	cells
T13538	29604-29613	JJ	denotes	embryonic
T13539	29620-29625	MD	denotes	could
T13540	29626-29628	VB	denotes	be
T13541	29629-29638	RB	denotes	partially
T13542	29649-29655	IN	denotes	during
T13543	29656-29665	NN	denotes	evolution
T13544	29665-29666	.	denotes	.
T13545	29666-29829	sentence	denotes	Indeed, in Arabidopsis thaliana, in which CAF-1 is not essential for viability [36], loss of CAF-1 is associated with a mild alteration in chromocenter size [37].
T13546	29667-29673	RB	denotes	Indeed
T13547	29769-29779	VBN	denotes	associated
T13548	29673-29675	,	denotes	,
T13549	29675-29677	IN	denotes	in
T13550	29678-29689	NNP	denotes	Arabidopsis
T13551	29690-29698	NNP	denotes	thaliana
T13552	29698-29700	,	denotes	,
T13553	29700-29702	IN	denotes	in
T13554	29715-29717	VBZ	denotes	is
T13555	29703-29708	WDT	denotes	which
T13556	29709-29712	NN	denotes	CAF
T13557	29712-29713	HYPH	denotes	-
T13558	29713-29714	CD	denotes	1
T13559	29718-29721	RB	denotes	not
T13560	29722-29731	JJ	denotes	essential
T13561	29732-29735	IN	denotes	for
T13562	29736-29745	NN	denotes	viability
T13563	29746-29747	-LRB-	denotes	[
T13564	29747-29749	CD	denotes	36
T13565	29749-29750	-RRB-	denotes	]
T13566	29750-29752	,	denotes	,
T13567	29752-29756	NN	denotes	loss
T13568	29757-29759	IN	denotes	of
T13569	29760-29763	NN	denotes	CAF
T13570	29763-29764	HYPH	denotes	-
T13571	29764-29765	CD	denotes	1
T13572	29766-29768	VBZ	denotes	is
T13573	29780-29784	IN	denotes	with
T13574	29785-29786	DT	denotes	a
T13575	29792-29802	NN	denotes	alteration
T13576	29787-29791	JJ	denotes	mild
T13577	29803-29805	IN	denotes	in
T13578	29806-29818	NN	denotes	chromocenter
T13579	29819-29823	NN	denotes	size
T13580	29824-29825	-LRB-	denotes	[
T13581	29825-29827	CD	denotes	37
T13582	29827-29828	-RRB-	denotes	]
T13583	29828-29829	.	denotes	.
T13584	29829-29957	sentence	denotes	This phenotype is somehow reminiscent of loss of CAF-1-mediated defects in heterochromatin organization in early mouse embryos.
T13585	29830-29834	DT	denotes	This
T13586	29835-29844	NN	denotes	phenotype
T13587	29845-29847	VBZ	denotes	is
T13588	29848-29855	RB	denotes	somehow
T13589	29856-29867	JJ	denotes	reminiscent
T13590	29868-29870	IN	denotes	of
T13591	29871-29875	NN	denotes	loss
T13592	29876-29878	IN	denotes	of
T13593	29879-29882	NN	denotes	CAF
T13594	29885-29893	VBN	denotes	mediated
T13595	29882-29883	HYPH	denotes	-
T13596	29883-29884	CD	denotes	1
T13597	29884-29885	HYPH	denotes	-
T13598	29894-29901	NNS	denotes	defects
T13599	29902-29904	IN	denotes	in
T13600	29905-29920	NN	denotes	heterochromatin
T13601	29921-29933	NN	denotes	organization
T13602	29934-29936	IN	denotes	in
T13603	29937-29942	JJ	denotes	early
T13604	29949-29956	NNS	denotes	embryos
T13605	29943-29948	NN	denotes	mouse
T13606	29956-29957	.	denotes	.
T13607	29957-30155	sentence	denotes	In mouse ES cells, we show that CAF-1 is also required for regular levels of H4K20me3 at pericentric repeats, but not at IAP elements, which are repeated sequences spread all over the mouse genome.
T13608	29958-29960	IN	denotes	In
T13609	29980-29984	VBP	denotes	show
T13610	29961-29966	NN	denotes	mouse
T13611	29970-29975	NNS	denotes	cells
T13612	29967-29969	NN	denotes	ES
T13613	29975-29977	,	denotes	,
T13614	29977-29979	PRP	denotes	we
T13615	29985-29989	IN	denotes	that
T13616	30004-30012	VBN	denotes	required
T13617	29990-29993	NN	denotes	CAF
T13618	29993-29994	HYPH	denotes	-
T13619	29994-29995	CD	denotes	1
T13620	29996-29998	VBZ	denotes	is
T13621	29999-30003	RB	denotes	also
T13622	30013-30016	IN	denotes	for
T13623	30017-30024	JJ	denotes	regular
T13624	30025-30031	NNS	denotes	levels
T13625	30032-30034	IN	denotes	of
T13626	30035-30043	NN	denotes	H4K20me3
T13627	30044-30046	IN	denotes	at
T13628	30047-30058	JJ	denotes	pericentric
T13629	30059-30066	NNS	denotes	repeats
T13630	30066-30068	,	denotes	,
T13631	30068-30071	CC	denotes	but
T13632	30072-30075	RB	denotes	not
T13633	30076-30078	IN	denotes	at
T13634	30079-30082	NN	denotes	IAP
T13635	30083-30091	NNS	denotes	elements
T13636	30091-30093	,	denotes	,
T13637	30093-30098	WDT	denotes	which
T13638	30099-30102	VBP	denotes	are
T13639	30103-30111	VBN	denotes	repeated
T13640	30112-30121	NNS	denotes	sequences
T13641	30122-30128	VBN	denotes	spread
T13642	30129-30132	RB	denotes	all
T13643	30133-30137	IN	denotes	over
T13644	30138-30141	DT	denotes	the
T13645	30148-30154	NN	denotes	genome
T13646	30142-30147	NN	denotes	mouse
T13647	30154-30155	.	denotes	.
T13648	30155-30286	sentence	denotes	These data suggest that proper nuclear organization and epigenetic marking of pericentric heterochromatin are coupled in ES cells.
T13649	30156-30161	DT	denotes	These
T13650	30162-30166	NNS	denotes	data
T13651	30167-30174	VBP	denotes	suggest
T13652	30175-30179	IN	denotes	that
T13653	30266-30273	VBN	denotes	coupled
T13654	30180-30186	JJ	denotes	proper
T13655	30195-30207	NN	denotes	organization
T13656	30187-30194	JJ	denotes	nuclear
T13657	30208-30211	CC	denotes	and
T13658	30212-30222	JJ	denotes	epigenetic
T13659	30223-30230	NN	denotes	marking
T13660	30231-30233	IN	denotes	of
T13661	30234-30245	JJ	denotes	pericentric
T13662	30246-30261	NN	denotes	heterochromatin
T13663	30262-30265	VBP	denotes	are
T13664	30274-30276	IN	denotes	in
T13665	30277-30279	NN	denotes	ES
T13666	30280-30285	NNS	denotes	cells
T13667	30285-30286	.	denotes	.
T13668	30286-30412	sentence	denotes	Alternatively, 3-D organization of heterochromatin and epigenetic marking might represent two independent functions of CAF-1.
T13669	30287-30300	RB	denotes	Alternatively
T13670	30367-30376	VB	denotes	represent
T13671	30300-30302	,	denotes	,
T13672	30302-30303	CD	denotes	3
T13673	30304-30305	NN	denotes	D
T13674	30303-30304	HYPH	denotes	-
T13675	30306-30318	NN	denotes	organization
T13676	30319-30321	IN	denotes	of
T13677	30322-30337	NN	denotes	heterochromatin
T13678	30338-30341	CC	denotes	and
T13679	30342-30352	JJ	denotes	epigenetic
T13680	30353-30360	NN	denotes	marking
T13681	30361-30366	MD	denotes	might
T13682	30377-30380	CD	denotes	two
T13683	30393-30402	NNS	denotes	functions
T13684	30381-30392	JJ	denotes	independent
T13685	30403-30405	IN	denotes	of
T13686	30406-30409	NN	denotes	CAF
T13687	30409-30410	HYPH	denotes	-
T13688	30410-30411	CD	denotes	1
T13689	30411-30412	.	denotes	.
T13690	30412-30588	sentence	denotes	The variable requirement for CAF-1 across species might reflect the acquisition of new properties affecting the function of histone H3 variants during evolution of eukaryotes.
T13691	30413-30416	DT	denotes	The
T13692	30426-30437	NN	denotes	requirement
T13693	30417-30425	JJ	denotes	variable
T13694	30469-30476	VB	denotes	reflect
T13695	30438-30441	IN	denotes	for
T13696	30442-30445	NN	denotes	CAF
T13697	30445-30446	HYPH	denotes	-
T13698	30446-30447	CD	denotes	1
T13699	30448-30454	IN	denotes	across
T13700	30455-30462	NNS	denotes	species
T13701	30463-30468	MD	denotes	might
T13702	30477-30480	DT	denotes	the
T13703	30481-30492	NN	denotes	acquisition
T13704	30493-30495	IN	denotes	of
T13705	30496-30499	JJ	denotes	new
T13706	30500-30510	NNS	denotes	properties
T13707	30511-30520	VBG	denotes	affecting
T13708	30521-30524	DT	denotes	the
T13709	30525-30533	NN	denotes	function
T13710	30534-30536	IN	denotes	of
T13711	30537-30544	NN	denotes	histone
T13712	30545-30547	NN	denotes	H3
T13713	30548-30556	NNS	denotes	variants
T13714	30557-30563	IN	denotes	during
T13715	30564-30573	NN	denotes	evolution
T13716	30574-30576	IN	denotes	of
T13717	30577-30587	NNS	denotes	eukaryotes
T13718	30587-30588	.	denotes	.
T13719	30588-30668	sentence	denotes	CAF-1 is essential in mammals but dispensable in Saccharomyces cerevisiae [38].
T13720	30589-30592	NN	denotes	CAF
T13721	30595-30597	VBZ	denotes	is
T13722	30592-30593	HYPH	denotes	-
T13723	30593-30594	CD	denotes	1
T13724	30598-30607	JJ	denotes	essential
T13725	30608-30610	IN	denotes	in
T13726	30611-30618	NNS	denotes	mammals
T13727	30619-30622	CC	denotes	but
T13728	30623-30634	JJ	denotes	dispensable
T13729	30635-30637	IN	denotes	in
T13730	30638-30651	NNP	denotes	Saccharomyces
T13731	30652-30662	NNP	denotes	cerevisiae
T13732	30663-30664	-LRB-	denotes	[
T13733	30664-30666	CD	denotes	38
T13734	30666-30667	-RRB-	denotes	]
T13735	30667-30668	.	denotes	.
T13736	30668-30956	sentence	denotes	Interestingly, the budding yeast genome does not contain heterochromatin regions equivalent to those of higher eukaryotes, and several hallmark features of heterochromatin such as di- and trimethylation at H3K9, H3K27, and H4K20 are not observed in the silent chromatin of S. cerevisiae.
T13737	30669-30682	RB	denotes	Interestingly
T13738	30718-30725	VB	denotes	contain
T13739	30682-30684	,	denotes	,
T13740	30684-30687	DT	denotes	the
T13741	30702-30708	NN	denotes	genome
T13742	30688-30695	VBG	denotes	budding
T13743	30696-30701	NN	denotes	yeast
T13744	30709-30713	VBZ	denotes	does
T13745	30714-30717	RB	denotes	not
T13746	30726-30741	NN	denotes	heterochromatin
T13747	30742-30749	NNS	denotes	regions
T13748	30750-30760	JJ	denotes	equivalent
T13749	30761-30763	IN	denotes	to
T13750	30764-30769	DT	denotes	those
T13751	30770-30772	IN	denotes	of
T13752	30773-30779	JJR	denotes	higher
T13753	30780-30790	NNS	denotes	eukaryotes
T13754	30790-30792	,	denotes	,
T13755	30792-30795	CC	denotes	and
T13756	30796-30803	JJ	denotes	several
T13757	30813-30821	NNS	denotes	features
T13758	30804-30812	NN	denotes	hallmark
T13759	30906-30914	VBN	denotes	observed
T13760	30822-30824	IN	denotes	of
T13761	30825-30840	NN	denotes	heterochromatin
T13762	30841-30845	JJ	denotes	such
T13763	30846-30848	IN	denotes	as
T13764	30849-30851	AFX	denotes	di
T13765	30857-30871	NN	denotes	trimethylation
T13766	30851-30852	HYPH	denotes	-
T13767	30853-30856	CC	denotes	and
T13768	30872-30874	IN	denotes	at
T13769	30875-30879	NN	denotes	H3K9
T13770	30879-30881	,	denotes	,
T13771	30881-30886	NN	denotes	H3K27
T13772	30886-30888	,	denotes	,
T13773	30888-30891	CC	denotes	and
T13774	30892-30897	NN	denotes	H4K20
T13775	30898-30901	VBP	denotes	are
T13776	30902-30905	RB	denotes	not
T13777	30915-30917	IN	denotes	in
T13778	30918-30921	DT	denotes	the
T13779	30929-30938	NN	denotes	chromatin
T13780	30922-30928	JJ	denotes	silent
T13781	30939-30941	IN	denotes	of
T13782	30942-30944	NNP	denotes	S.
T13783	30945-30955	NNP	denotes	cerevisiae
T13784	30955-30956	.	denotes	.
T13785	30956-31128	sentence	denotes	In mammals, H3K9 dimethylation is predominantly associated with H3.1, but not with H3.3, which carries modifications typically found in transcribed chromatin regions [39].
T13786	30957-30959	IN	denotes	In
T13787	31005-31015	VBN	denotes	associated
T13788	30960-30967	NNS	denotes	mammals
T13789	30967-30969	,	denotes	,
T13790	30969-30973	NN	denotes	H3K9
T13791	30974-30987	NN	denotes	dimethylation
T13792	30988-30990	VBZ	denotes	is
T13793	30991-31004	RB	denotes	predominantly
T13794	31016-31020	IN	denotes	with
T13795	31021-31025	NN	denotes	H3.1
T13796	31025-31027	,	denotes	,
T13797	31027-31030	CC	denotes	but
T13798	31031-31034	RB	denotes	not
T13799	31035-31039	IN	denotes	with
T13800	31040-31044	NN	denotes	H3.3
T13801	31044-31046	,	denotes	,
T13802	31046-31051	WDT	denotes	which
T13803	31052-31059	VBZ	denotes	carries
T13804	31060-31073	NNS	denotes	modifications
T13805	31074-31083	RB	denotes	typically
T13806	31084-31089	VBN	denotes	found
T13807	31090-31092	IN	denotes	in
T13808	31093-31104	VBN	denotes	transcribed
T13809	31115-31122	NNS	denotes	regions
T13810	31105-31114	NN	denotes	chromatin
T13811	31123-31124	-LRB-	denotes	[
T13812	31124-31126	CD	denotes	39
T13813	31126-31127	-RRB-	denotes	]
T13814	31127-31128	.	denotes	.
T13815	31128-31317	sentence	denotes	Outside of the centromeric H3 variant, S. cerevisiae possesses only H3.3, which is expressed and incorporated into chromatin in a replication-independent fashion in higher eukaryotes [12].
T13816	31129-31136	IN	denotes	Outside
T13817	31182-31191	VBZ	denotes	possesses
T13818	31137-31139	IN	denotes	of
T13819	31140-31143	DT	denotes	the
T13820	31159-31166	NN	denotes	variant
T13821	31144-31155	JJ	denotes	centromeric
T13822	31156-31158	NN	denotes	H3
T13823	31166-31168	,	denotes	,
T13824	31168-31170	NNP	denotes	S.
T13825	31171-31181	NNP	denotes	cerevisiae
T13826	31192-31196	RB	denotes	only
T13827	31197-31201	NN	denotes	H3.3
T13828	31201-31203	,	denotes	,
T13829	31203-31208	WDT	denotes	which
T13830	31212-31221	VBN	denotes	expressed
T13831	31209-31211	VBZ	denotes	is
T13832	31222-31225	CC	denotes	and
T13833	31226-31238	VBN	denotes	incorporated
T13834	31239-31243	IN	denotes	into
T13835	31244-31253	NN	denotes	chromatin
T13836	31254-31256	IN	denotes	in
T13837	31257-31258	DT	denotes	a
T13838	31283-31290	NN	denotes	fashion
T13839	31259-31270	NN	denotes	replication
T13840	31271-31282	JJ	denotes	independent
T13841	31270-31271	HYPH	denotes	-
T13842	31291-31293	IN	denotes	in
T13843	31294-31300	JJR	denotes	higher
T13844	31301-31311	NNS	denotes	eukaryotes
T13845	31312-31313	-LRB-	denotes	[
T13846	31313-31315	CD	denotes	12
T13847	31315-31316	-RRB-	denotes	]
T13848	31316-31317	.	denotes	.
T13849	31317-31389	sentence	denotes	These data suggest a specific role for H3.1 in heterochromatin regions.
T13850	31318-31323	DT	denotes	These
T13851	31324-31328	NNS	denotes	data
T13852	31329-31336	VBP	denotes	suggest
T13853	31337-31338	DT	denotes	a
T13854	31348-31352	NN	denotes	role
T13855	31339-31347	JJ	denotes	specific
T13856	31353-31356	IN	denotes	for
T13857	31357-31361	NN	denotes	H3.1
T13858	31362-31364	IN	denotes	in
T13859	31365-31380	NN	denotes	heterochromatin
T13860	31381-31388	NNS	denotes	regions
T13861	31388-31389	.	denotes	.
T13862	31389-31584	sentence	denotes	We show here that CAF-1, which is a H3.1 replication-dependent loading complex [11], is specifically required for the nuclear organization of pericentric heterochromatin in embryos and ES cells.
T13863	31390-31392	PRP	denotes	We
T13864	31393-31397	VBP	denotes	show
T13865	31398-31402	RB	denotes	here
T13866	31403-31407	IN	denotes	that
T13867	31491-31499	VBN	denotes	required
T13868	31408-31411	NN	denotes	CAF
T13869	31411-31412	HYPH	denotes	-
T13870	31412-31413	CD	denotes	1
T13871	31413-31415	,	denotes	,
T13872	31415-31420	WDT	denotes	which
T13873	31421-31423	VBZ	denotes	is
T13874	31424-31425	DT	denotes	a
T13875	31461-31468	NN	denotes	complex
T13876	31426-31430	NN	denotes	H3.1
T13877	31431-31442	NN	denotes	replication
T13878	31443-31452	JJ	denotes	dependent
T13879	31442-31443	HYPH	denotes	-
T13880	31453-31460	NN	denotes	loading
T13881	31469-31470	-LRB-	denotes	[
T13882	31470-31472	CD	denotes	11
T13883	31472-31473	-RRB-	denotes	]
T13884	31473-31475	,	denotes	,
T13885	31475-31477	VBZ	denotes	is
T13886	31478-31490	RB	denotes	specifically
T13887	31500-31503	IN	denotes	for
T13888	31504-31507	DT	denotes	the
T13889	31516-31528	NN	denotes	organization
T13890	31508-31515	JJ	denotes	nuclear
T13891	31529-31531	IN	denotes	of
T13892	31532-31543	JJ	denotes	pericentric
T13893	31544-31559	NN	denotes	heterochromatin
T13894	31560-31562	IN	denotes	in
T13895	31563-31570	NNS	denotes	embryos
T13896	31571-31574	CC	denotes	and
T13897	31575-31577	NN	denotes	ES
T13898	31578-31583	NNS	denotes	cells
T13899	31583-31584	.	denotes	.
T13900	31584-31771	sentence	denotes	One possibility would be a scenario in which incorporation of H3.1 into chromatin is required for the proper nuclear organization of constitutive heterochromatin in an embryonic context.
T13901	31585-31588	CD	denotes	One
T13902	31589-31600	NN	denotes	possibility
T13903	31607-31609	VB	denotes	be
T13904	31601-31606	MD	denotes	would
T13905	31610-31611	DT	denotes	a
T13906	31612-31620	NN	denotes	scenario
T13907	31621-31623	IN	denotes	in
T13908	31670-31678	VBN	denotes	required
T13909	31624-31629	WDT	denotes	which
T13910	31630-31643	NN	denotes	incorporation
T13911	31644-31646	IN	denotes	of
T13912	31647-31651	NN	denotes	H3.1
T13913	31652-31656	IN	denotes	into
T13914	31657-31666	NN	denotes	chromatin
T13915	31667-31669	VBZ	denotes	is
T13916	31679-31682	IN	denotes	for
T13917	31683-31686	DT	denotes	the
T13918	31702-31714	NN	denotes	organization
T13919	31687-31693	JJ	denotes	proper
T13920	31694-31701	JJ	denotes	nuclear
T13921	31715-31717	IN	denotes	of
T13922	31718-31730	JJ	denotes	constitutive
T13923	31731-31746	NN	denotes	heterochromatin
T13924	31747-31749	IN	denotes	in
T13925	31750-31752	DT	denotes	an
T13926	31763-31770	NN	denotes	context
T13927	31753-31762	JJ	denotes	embryonic
T13928	31770-31771	.	denotes	.
T13929	31771-31979	sentence	denotes	While this has to be addressed experimentally, it is worthwhile to mention an alternate possible mechanism that might explain the disruption of heterochromatin domains in p150CAF-1 null embryos and ES cells.
T13930	31772-31777	IN	denotes	While
T13931	31783-31786	VBZ	denotes	has
T13932	31778-31782	DT	denotes	this
T13933	31822-31824	VBZ	denotes	is
T13934	31787-31789	TO	denotes	to
T13935	31793-31802	VBN	denotes	addressed
T13936	31790-31792	VB	denotes	be
T13937	31803-31817	RB	denotes	experimentally
T13938	31817-31819	,	denotes	,
T13939	31819-31821	PRP	denotes	it
T13940	31825-31835	JJ	denotes	worthwhile
T13941	31836-31838	TO	denotes	to
T13942	31839-31846	VB	denotes	mention
T13943	31847-31849	DT	denotes	an
T13944	31869-31878	NN	denotes	mechanism
T13945	31850-31859	JJ	denotes	alternate
T13946	31860-31868	JJ	denotes	possible
T13947	31879-31883	WDT	denotes	that
T13948	31890-31897	VB	denotes	explain
T13949	31884-31889	MD	denotes	might
T13950	31898-31901	DT	denotes	the
T13951	31902-31912	NN	denotes	disruption
T13952	31913-31915	IN	denotes	of
T13953	31916-31931	NN	denotes	heterochromatin
T13954	31932-31939	NNS	denotes	domains
T13955	31940-31942	IN	denotes	in
T13956	31943-31950	NN	denotes	p150CAF
T13957	31953-31957	JJ	denotes	null
T13958	31950-31951	HYPH	denotes	-
T13959	31951-31952	CD	denotes	1
T13960	31958-31965	NNS	denotes	embryos
T13961	31966-31969	CC	denotes	and
T13962	31970-31972	NN	denotes	ES
T13963	31973-31978	NNS	denotes	cells
T13964	31978-31979	.	denotes	.
T13965	31979-32120	sentence	denotes	CAF-1 could be required for the loading, into heterochromatin, of an interacting partner required for the clustering of pericentric domains.
T13966	31980-31983	NN	denotes	CAF
T13967	31995-32003	VBN	denotes	required
T13968	31983-31984	HYPH	denotes	-
T13969	31984-31985	CD	denotes	1
T13970	31986-31991	MD	denotes	could
T13971	31992-31994	VB	denotes	be
T13972	32004-32007	IN	denotes	for
T13973	32008-32011	DT	denotes	the
T13974	32012-32019	NN	denotes	loading
T13975	32019-32021	,	denotes	,
T13976	32021-32025	IN	denotes	into
T13977	32026-32041	NN	denotes	heterochromatin
T13978	32041-32043	,	denotes	,
T13979	32043-32045	IN	denotes	of
T13980	32046-32048	DT	denotes	an
T13981	32061-32068	NN	denotes	partner
T13982	32049-32060	VBG	denotes	interacting
T13983	32069-32077	VBN	denotes	required
T13984	32078-32081	IN	denotes	for
T13985	32082-32085	DT	denotes	the
T13986	32086-32096	NN	denotes	clustering
T13987	32097-32099	IN	denotes	of
T13988	32100-32111	JJ	denotes	pericentric
T13989	32112-32119	NNS	denotes	domains
T13990	32119-32120	.	denotes	.
T13991	32120-32310	sentence	denotes	Following one or two rounds of DNA replication in the absence of p150CAF-1, heterochromatin would become deprived of this molecule, which would cause the disruption of its 3-D organization.
T13992	32121-32130	VBG	denotes	Following
T13993	32219-32225	VB	denotes	become
T13994	32131-32134	CD	denotes	one
T13995	32142-32148	NNS	denotes	rounds
T13996	32135-32137	CC	denotes	or
T13997	32138-32141	CD	denotes	two
T13998	32149-32151	IN	denotes	of
T13999	32152-32155	NN	denotes	DNA
T14000	32156-32167	NN	denotes	replication
T14001	32168-32170	IN	denotes	in
T14002	32171-32174	DT	denotes	the
T14003	32175-32182	NN	denotes	absence
T14004	32183-32185	IN	denotes	of
T14005	32186-32193	NN	denotes	p150CAF
T14006	32193-32194	HYPH	denotes	-
T14007	32194-32195	CD	denotes	1
T14008	32195-32197	,	denotes	,
T14009	32197-32212	NN	denotes	heterochromatin
T14010	32213-32218	MD	denotes	would
T14011	32226-32234	JJ	denotes	deprived
T14012	32235-32237	IN	denotes	of
T14013	32238-32242	DT	denotes	this
T14014	32243-32251	NN	denotes	molecule
T14015	32251-32253	,	denotes	,
T14016	32253-32258	WDT	denotes	which
T14017	32265-32270	VB	denotes	cause
T14018	32259-32264	MD	denotes	would
T14019	32271-32274	DT	denotes	the
T14020	32275-32285	NN	denotes	disruption
T14021	32286-32288	IN	denotes	of
T14022	32289-32292	PRP$	denotes	its
T14023	32297-32309	NN	denotes	organization
T14024	32293-32294	CD	denotes	3
T14025	32295-32296	NN	denotes	D
T14026	32294-32295	HYPH	denotes	-
T14027	32309-32310	.	denotes	.
T14028	32310-32388	sentence	denotes	A potential candidate is the HP1 protein, which interacts with CAF-1 [13,16].
T14029	32311-32312	DT	denotes	A
T14030	32323-32332	NN	denotes	candidate
T14031	32313-32322	JJ	denotes	potential
T14032	32333-32335	VBZ	denotes	is
T14033	32336-32339	DT	denotes	the
T14034	32344-32351	NN	denotes	protein
T14035	32340-32343	NN	denotes	HP1
T14036	32351-32353	,	denotes	,
T14037	32353-32358	WDT	denotes	which
T14038	32359-32368	VBZ	denotes	interacts
T14039	32369-32373	IN	denotes	with
T14040	32374-32377	NN	denotes	CAF
T14041	32377-32378	HYPH	denotes	-
T14042	32378-32379	CD	denotes	1
T14043	32380-32381	-LRB-	denotes	[
T14044	32384-32386	CD	denotes	16
T14045	32381-32383	CD	denotes	13
T14046	32383-32384	,	denotes	,
T14047	32386-32387	-RRB-	denotes	]
T14048	32387-32388	.	denotes	.
T14049	32388-32585	sentence	denotes	In agreement with a recent report [40], our analysis of heterochromatin in pre-implantation embryos has revealed drastic changes in nuclear organization between the two-cell and blastocyst stages.
T14050	32389-32391	IN	denotes	In
T14051	32493-32501	VBN	denotes	revealed
T14052	32392-32401	NN	denotes	agreement
T14053	32402-32406	IN	denotes	with
T14054	32407-32408	DT	denotes	a
T14055	32416-32422	NN	denotes	report
T14056	32409-32415	JJ	denotes	recent
T14057	32423-32424	-LRB-	denotes	[
T14058	32424-32426	CD	denotes	40
T14059	32426-32427	-RRB-	denotes	]
T14060	32427-32429	,	denotes	,
T14061	32429-32432	PRP$	denotes	our
T14062	32433-32441	NN	denotes	analysis
T14063	32442-32444	IN	denotes	of
T14064	32445-32460	NN	denotes	heterochromatin
T14065	32461-32463	IN	denotes	in
T14066	32464-32480	JJ	denotes	pre-implantation
T14067	32481-32488	NNS	denotes	embryos
T14068	32489-32492	VBZ	denotes	has
T14069	32502-32509	JJ	denotes	drastic
T14070	32510-32517	NNS	denotes	changes
T14071	32518-32520	IN	denotes	in
T14072	32521-32528	JJ	denotes	nuclear
T14073	32529-32541	NN	denotes	organization
T14074	32542-32549	IN	denotes	between
T14075	32550-32553	DT	denotes	the
T14076	32578-32584	NNS	denotes	stages
T14077	32554-32557	CD	denotes	two
T14078	32558-32562	NN	denotes	cell
T14079	32557-32558	HYPH	denotes	-
T14080	32563-32566	CC	denotes	and
T14081	32567-32577	NN	denotes	blastocyst
T14082	32584-32585	.	denotes	.
T14083	32585-32778	sentence	denotes	Cloning experiments in mouse revealed that the nuclear organization of heterochromatin in ES cell nuclei was quickly reverted into the one-cell stage-specific form after nuclear transfer [40].
T14084	32586-32593	NN	denotes	Cloning
T14085	32594-32605	NNS	denotes	experiments
T14086	32615-32623	VBD	denotes	revealed
T14087	32606-32608	IN	denotes	in
T14088	32609-32614	NN	denotes	mouse
T14089	32624-32628	IN	denotes	that
T14090	32703-32711	VBN	denotes	reverted
T14091	32629-32632	DT	denotes	the
T14092	32641-32653	NN	denotes	organization
T14093	32633-32640	JJ	denotes	nuclear
T14094	32654-32656	IN	denotes	of
T14095	32657-32672	NN	denotes	heterochromatin
T14096	32673-32675	IN	denotes	in
T14097	32676-32678	NN	denotes	ES
T14098	32684-32690	NNS	denotes	nuclei
T14099	32679-32683	NN	denotes	cell
T14100	32691-32694	VBD	denotes	was
T14101	32695-32702	RB	denotes	quickly
T14102	32712-32716	IN	denotes	into
T14103	32717-32720	DT	denotes	the
T14104	32745-32749	NN	denotes	form
T14105	32721-32724	CD	denotes	one
T14106	32725-32729	NN	denotes	cell
T14107	32724-32725	HYPH	denotes	-
T14108	32730-32735	NN	denotes	stage
T14109	32736-32744	JJ	denotes	specific
T14110	32735-32736	HYPH	denotes	-
T14111	32750-32755	IN	denotes	after
T14112	32756-32763	JJ	denotes	nuclear
T14113	32764-32772	NN	denotes	transfer
T14114	32773-32774	-LRB-	denotes	[
T14115	32774-32776	CD	denotes	40
T14116	32776-32777	-RRB-	denotes	]
T14117	32777-32778	.	denotes	.
T14118	32778-32916	sentence	denotes	Hence, remodeling of heterochromatin 3-D organization parallels nuclear reprogramming toward an early embryonic status in cloned embryos.
T14119	32779-32784	RB	denotes	Hence
T14120	32833-32842	VBZ	denotes	parallels
T14121	32784-32786	,	denotes	,
T14122	32786-32796	NN	denotes	remodeling
T14123	32797-32799	IN	denotes	of
T14124	32800-32815	NN	denotes	heterochromatin
T14125	32820-32832	NN	denotes	organization
T14126	32816-32817	CD	denotes	3
T14127	32818-32819	NN	denotes	D
T14128	32817-32818	HYPH	denotes	-
T14129	32843-32850	JJ	denotes	nuclear
T14130	32851-32864	NN	denotes	reprogramming
T14131	32865-32871	IN	denotes	toward
T14132	32872-32874	DT	denotes	an
T14133	32891-32897	NN	denotes	status
T14134	32875-32880	JJ	denotes	early
T14135	32881-32890	JJ	denotes	embryonic
T14136	32898-32900	IN	denotes	in
T14137	32901-32907	VBN	denotes	cloned
T14138	32908-32915	NNS	denotes	embryos
T14139	32915-32916	.	denotes	.
T14140	32916-33101	sentence	denotes	This drastic reorganization of heterochromatin organization is similar to that observed in p150CAF-1-depleted ES cells, and in developmentally arrested 16-cell stage Chaf1a−/− embryos.
T14141	32917-32921	DT	denotes	This
T14142	32930-32944	NN	denotes	reorganization
T14143	32922-32929	JJ	denotes	drastic
T14144	32977-32979	VBZ	denotes	is
T14145	32945-32947	IN	denotes	of
T14146	32948-32963	NN	denotes	heterochromatin
T14147	32964-32976	NN	denotes	organization
T14148	32980-32987	JJ	denotes	similar
T14149	32988-32990	IN	denotes	to
T14150	32991-32995	DT	denotes	that
T14151	32996-33004	VBN	denotes	observed
T14152	33005-33007	IN	denotes	in
T14153	33008-33015	NN	denotes	p150CAF
T14154	33018-33026	VBN	denotes	depleted
T14155	33015-33016	HYPH	denotes	-
T14156	33016-33017	CD	denotes	1
T14157	33017-33018	HYPH	denotes	-
T14158	33030-33035	NNS	denotes	cells
T14159	33027-33029	NN	denotes	ES
T14160	33035-33037	,	denotes	,
T14161	33037-33040	CC	denotes	and
T14162	33041-33043	IN	denotes	in
T14163	33044-33059	RB	denotes	developmentally
T14164	33060-33068	VBN	denotes	arrested
T14165	33093-33100	NNS	denotes	embryos
T14166	33069-33071	CD	denotes	16
T14167	33072-33076	NN	denotes	cell
T14168	33071-33072	HYPH	denotes	-
T14169	33077-33082	NN	denotes	stage
T14170	33083-33089	NN	denotes	Chaf1a
T14171	33089-33090	SYM	denotes	−
T14172	33090-33091	HYPH	denotes	/
T14173	33091-33092	SYM	denotes	−
T14174	33100-33101	.	denotes	.
T14175	33101-33241	sentence	denotes	These data show that CAF-1 is involved in setting up proper heterochromatin architecture during the first cell divisions of embryonic life.
T14176	33102-33107	DT	denotes	These
T14177	33108-33112	NNS	denotes	data
T14178	33113-33117	VBP	denotes	show
T14179	33118-33122	IN	denotes	that
T14180	33132-33140	VBN	denotes	involved
T14181	33123-33126	NN	denotes	CAF
T14182	33126-33127	HYPH	denotes	-
T14183	33127-33128	CD	denotes	1
T14184	33129-33131	VBZ	denotes	is
T14185	33141-33143	IN	denotes	in
T14186	33144-33151	VBG	denotes	setting
T14187	33152-33154	RP	denotes	up
T14188	33155-33161	JJ	denotes	proper
T14189	33178-33190	NN	denotes	architecture
T14190	33162-33177	NN	denotes	heterochromatin
T14191	33191-33197	IN	denotes	during
T14192	33198-33201	DT	denotes	the
T14193	33213-33222	NNS	denotes	divisions
T14194	33202-33207	JJ	denotes	first
T14195	33208-33212	NN	denotes	cell
T14196	33223-33225	IN	denotes	of
T14197	33226-33235	JJ	denotes	embryonic
T14198	33236-33240	NN	denotes	life
T14199	33240-33241	.	denotes	.
T14200	33241-33449	sentence	denotes	Given the importance of nuclear organization in the control of gene expression [21,41], this finding suggests that CAF-1 contributes to the coordinated programs of gene expression during early embryogenesis.
T14201	33242-33247	VBN	denotes	Given
T14202	33343-33351	VBZ	denotes	suggests
T14203	33248-33251	DT	denotes	the
T14204	33252-33262	NN	denotes	importance
T14205	33263-33265	IN	denotes	of
T14206	33266-33273	JJ	denotes	nuclear
T14207	33274-33286	NN	denotes	organization
T14208	33287-33289	IN	denotes	in
T14209	33290-33293	DT	denotes	the
T14210	33294-33301	NN	denotes	control
T14211	33302-33304	IN	denotes	of
T14212	33305-33309	NN	denotes	gene
T14213	33310-33320	NN	denotes	expression
T14214	33321-33322	-LRB-	denotes	[
T14215	33325-33327	CD	denotes	41
T14216	33322-33324	CD	denotes	21
T14217	33324-33325	,	denotes	,
T14218	33327-33328	-RRB-	denotes	]
T14219	33328-33330	,	denotes	,
T14220	33330-33334	DT	denotes	this
T14221	33335-33342	NN	denotes	finding
T14222	33352-33356	IN	denotes	that
T14223	33363-33374	VBZ	denotes	contributes
T14224	33357-33360	NN	denotes	CAF
T14225	33360-33361	HYPH	denotes	-
T14226	33361-33362	CD	denotes	1
T14227	33375-33377	IN	denotes	to
T14228	33378-33381	DT	denotes	the
T14229	33394-33402	NNS	denotes	programs
T14230	33382-33393	VBN	denotes	coordinated
T14231	33403-33405	IN	denotes	of
T14232	33406-33410	NN	denotes	gene
T14233	33411-33421	NN	denotes	expression
T14234	33422-33428	IN	denotes	during
T14235	33429-33434	JJ	denotes	early
T14236	33435-33448	NN	denotes	embryogenesis
T14237	33448-33449	.	denotes	.
T14238	33449-33588	sentence	denotes	Our findings open new perspectives in the understanding of chromatin dynamics during early development and in pluripotent embryonic cells.
T14239	33450-33453	PRP$	denotes	Our
T14240	33454-33462	NNS	denotes	findings
T14241	33463-33467	VBP	denotes	open
T14242	33468-33471	JJ	denotes	new
T14243	33472-33484	NNS	denotes	perspectives
T14244	33485-33487	IN	denotes	in
T14245	33488-33491	DT	denotes	the
T14246	33492-33505	NN	denotes	understanding
T14247	33506-33508	IN	denotes	of
T14248	33509-33518	NN	denotes	chromatin
T14249	33519-33527	NNS	denotes	dynamics
T14250	33528-33534	IN	denotes	during
T14251	33535-33540	JJ	denotes	early
T14252	33541-33552	NN	denotes	development
T14253	33553-33556	CC	denotes	and
T14254	33557-33559	IN	denotes	in
T14255	33560-33571	JJ	denotes	pluripotent
T14256	33582-33587	NNS	denotes	cells
T14257	33572-33581	JJ	denotes	embryonic
T14258	33587-33588	.	denotes	.
T14259	33588-33751	sentence	denotes	While most studies focused on the role of DNA methylation and histone modifying enzymes [3], our data point to the importance of the chromatin assembly machinery.
T14260	33589-33594	IN	denotes	While
T14261	33608-33615	VBD	denotes	focused
T14262	33595-33599	JJS	denotes	most
T14263	33600-33607	NNS	denotes	studies
T14264	33691-33696	VBP	denotes	point
T14265	33616-33618	IN	denotes	on
T14266	33619-33622	DT	denotes	the
T14267	33623-33627	NN	denotes	role
T14268	33628-33630	IN	denotes	of
T14269	33631-33634	NN	denotes	DNA
T14270	33635-33646	NN	denotes	methylation
T14271	33647-33650	CC	denotes	and
T14272	33651-33658	NN	denotes	histone
T14273	33659-33668	VBG	denotes	modifying
T14274	33669-33676	NNS	denotes	enzymes
T14275	33677-33678	-LRB-	denotes	[
T14276	33678-33679	CD	denotes	3
T14277	33679-33680	-RRB-	denotes	]
T14278	33680-33682	,	denotes	,
T14279	33682-33685	PRP$	denotes	our
T14280	33686-33690	NNS	denotes	data
T14281	33697-33699	IN	denotes	to
T14282	33700-33703	DT	denotes	the
T14283	33704-33714	NN	denotes	importance
T14284	33715-33717	IN	denotes	of
T14285	33718-33721	DT	denotes	the
T14286	33741-33750	NN	denotes	machinery
T14287	33722-33731	NN	denotes	chromatin
T14288	33732-33740	NN	denotes	assembly
T14289	33750-33751	.	denotes	.
T14290	33751-33911	sentence	denotes	We show that in addition to assembling nucleosomes, CAF-1 provides spatial and epigenetic information to heterochromatin domains in early embryos and ES cells.
T14291	33752-33754	PRP	denotes	We
T14292	33755-33759	VBP	denotes	show
T14293	33760-33764	IN	denotes	that
T14294	33810-33818	VBZ	denotes	provides
T14295	33765-33767	IN	denotes	in
T14296	33768-33776	NN	denotes	addition
T14297	33777-33779	IN	denotes	to
T14298	33780-33790	VBG	denotes	assembling
T14299	33791-33802	NNS	denotes	nucleosomes
T14300	33802-33804	,	denotes	,
T14301	33804-33807	NN	denotes	CAF
T14302	33807-33808	HYPH	denotes	-
T14303	33808-33809	CD	denotes	1
T14304	33819-33826	JJ	denotes	spatial
T14305	33842-33853	NN	denotes	information
T14306	33827-33830	CC	denotes	and
T14307	33831-33841	JJ	denotes	epigenetic
T14308	33854-33856	IN	denotes	to
T14309	33857-33872	NN	denotes	heterochromatin
T14310	33873-33880	NNS	denotes	domains
T14311	33881-33883	IN	denotes	in
T14312	33884-33889	JJ	denotes	early
T14313	33890-33897	NNS	denotes	embryos
T14314	33898-33901	CC	denotes	and
T14315	33902-33904	NN	denotes	ES
T14316	33905-33910	NNS	denotes	cells
T14317	33910-33911	.	denotes	.
T14828	33936-33946	NN	denotes	Generation
T14829	33947-33949	IN	denotes	of
T14830	33950-33956	NN	denotes	Chaf1a
T14831	33957-33963	NN	denotes	mutant
T14832	33964-33968	NNS	denotes	mice
T14833	33969-33972	CC	denotes	and
T14834	33973-33980	NNS	denotes	embryos
T14835	33980-33981	.	denotes	.
T14836	33981-34069	sentence	denotes	Using PCR, we amplified two genomic fragments (about 3 kb each) flanking Chaf1a exon 3.
T14837	33982-33987	VBG	denotes	Using
T14838	33996-34005	VBD	denotes	amplified
T14839	33988-33991	NN	denotes	PCR
T14840	33991-33993	,	denotes	,
T14841	33993-33995	PRP	denotes	we
T14842	34006-34009	CD	denotes	two
T14843	34018-34027	NNS	denotes	fragments
T14844	34010-34017	JJ	denotes	genomic
T14845	34028-34029	-LRB-	denotes	(
T14846	34037-34039	NN	denotes	kb
T14847	34029-34034	IN	denotes	about
T14848	34035-34036	CD	denotes	3
T14849	34040-34044	DT	denotes	each
T14850	34044-34045	-RRB-	denotes	)
T14851	34046-34054	VBG	denotes	flanking
T14852	34055-34061	NN	denotes	Chaf1a
T14853	34062-34066	NN	denotes	exon
T14854	34067-34068	CD	denotes	3
T14855	34068-34069	.	denotes	.
T14856	34069-34210	sentence	denotes	These DNA fragments were assembled by conventional cloning with the neomycin and DT (diphtheria toxin) cassettes, as described in Figure 1A.
T14857	34070-34075	DT	denotes	These
T14858	34080-34089	NNS	denotes	fragments
T14859	34076-34079	NN	denotes	DNA
T14860	34095-34104	VBN	denotes	assembled
T14861	34090-34094	VBD	denotes	were
T14862	34105-34107	IN	denotes	by
T14863	34108-34120	JJ	denotes	conventional
T14864	34121-34128	NN	denotes	cloning
T14865	34129-34133	IN	denotes	with
T14866	34134-34137	DT	denotes	the
T14867	34173-34182	NNS	denotes	cassettes
T14868	34138-34146	NN	denotes	neomycin
T14869	34147-34150	CC	denotes	and
T14870	34151-34153	NN	denotes	DT
T14871	34154-34155	-LRB-	denotes	(
T14872	34155-34165	NN	denotes	diphtheria
T14873	34166-34171	NN	denotes	toxin
T14874	34171-34172	-RRB-	denotes	)
T14875	34182-34184	,	denotes	,
T14876	34184-34186	IN	denotes	as
T14877	34187-34196	VBN	denotes	described
T14878	34197-34199	IN	denotes	in
T14879	34200-34206	NN	denotes	Figure
T14880	34207-34209	NN	denotes	1A
T14881	34209-34210	.	denotes	.
T14882	34210-34272	sentence	denotes	The construct was transfected in ES cells by electroporation.
T14883	34211-34214	DT	denotes	The
T14884	34215-34224	NN	denotes	construct
T14885	34229-34240	VBN	denotes	transfected
T14886	34225-34228	VBD	denotes	was
T14887	34241-34243	IN	denotes	in
T14888	34244-34246	NN	denotes	ES
T14889	34247-34252	NNS	denotes	cells
T14890	34253-34255	IN	denotes	by
T14891	34256-34271	NN	denotes	electroporation
T14892	34271-34272	.	denotes	.
T14893	34272-34419	sentence	denotes	We identified recombinant ES cells carrying the mutant Chaf1a tm1Ger (abbreviated Chaf1a − in the manuscript) allele by Southern blot (Figure 1A).
T14894	34273-34275	PRP	denotes	We
T14895	34276-34286	VBD	denotes	identified
T14896	34287-34298	JJ	denotes	recombinant
T14897	34302-34307	NNS	denotes	cells
T14898	34299-34301	NN	denotes	ES
T14899	34308-34316	VBG	denotes	carrying
T14900	34317-34320	DT	denotes	the
T14901	34383-34389	NN	denotes	allele
T14902	34321-34327	NN	denotes	mutant
T14903	34328-34341	NN	denotes	Chaf1a tm1Ger
T14904	34342-34343	-LRB-	denotes	(
T14905	34343-34354	VBN	denotes	abbreviated
T14906	34355-34361	NN	denotes	Chaf1a
T14907	34362-34363	SYM	denotes	−
T14908	34364-34366	IN	denotes	in
T14909	34367-34370	DT	denotes	the
T14910	34371-34381	NN	denotes	manuscript
T14911	34381-34382	-RRB-	denotes	)
T14912	34390-34392	IN	denotes	by
T14913	34393-34401	NNP	denotes	Southern
T14914	34402-34406	NN	denotes	blot
T14915	34407-34408	-LRB-	denotes	(
T14916	34415-34417	NN	denotes	1A
T14917	34408-34414	NN	denotes	Figure
T14918	34417-34418	-RRB-	denotes	)
T14919	34418-34419	.	denotes	.
T14920	34419-34506	sentence	denotes	We derived Chaf1a +/− mice by injecting recombined ES cells into C57BL/6N blastocysts.
T14921	34420-34422	PRP	denotes	We
T14922	34423-34430	VBD	denotes	derived
T14923	34431-34437	NN	denotes	Chaf1a
T14924	34442-34446	NNS	denotes	mice
T14925	34438-34439	SYM	denotes	+
T14926	34439-34440	HYPH	denotes	/
T14927	34440-34441	SYM	denotes	−
T14928	34447-34449	IN	denotes	by
T14929	34450-34459	VBG	denotes	injecting
T14930	34460-34470	VBN	denotes	recombined
T14931	34474-34479	NNS	denotes	cells
T14932	34471-34473	NN	denotes	ES
T14933	34480-34484	IN	denotes	into
T14934	34485-34490	NN	denotes	C57BL
T14935	34491-34493	NN	denotes	6N
T14936	34490-34491	HYPH	denotes	/
T14937	34494-34505	NNS	denotes	blastocysts
T14938	34505-34506	.	denotes	.
T14939	34506-34609	sentence	denotes	E4 embryos obtained from the intercross of Chaf1a +/− mice were genotyped by nested-PCR amplification.
T14940	34507-34509	NN	denotes	E4
T14941	34510-34517	NNS	denotes	embryos
T14942	34571-34580	VBN	denotes	genotyped
T14943	34518-34526	VBN	denotes	obtained
T14944	34527-34531	IN	denotes	from
T14945	34532-34535	DT	denotes	the
T14946	34536-34546	NN	denotes	intercross
T14947	34547-34549	IN	denotes	of
T14948	34550-34556	NN	denotes	Chaf1a
T14949	34561-34565	NNS	denotes	mice
T14950	34557-34558	SYM	denotes	+
T14951	34558-34559	HYPH	denotes	/
T14952	34559-34560	SYM	denotes	−
T14953	34566-34570	VBD	denotes	were
T14954	34581-34583	IN	denotes	by
T14955	34584-34590	NN	denotes	nested
T14956	34591-34594	NN	denotes	PCR
T14957	34590-34591	HYPH	denotes	-
T14958	34595-34608	NN	denotes	amplification
T14959	34608-34609	.	denotes	.
T14960	34609-34760	sentence	denotes	Embryos were collected in a PCR reaction mix containing oligonucleotide primers 1, 2, and 3 (Figure 1A) that amplify the wild-type and mutant alleles.
T14961	34610-34617	NNS	denotes	Embryos
T14962	34623-34632	VBN	denotes	collected
T14963	34618-34622	VBD	denotes	were
T14964	34633-34635	IN	denotes	in
T14965	34636-34637	DT	denotes	a
T14966	34651-34654	NN	denotes	mix
T14967	34638-34641	NN	denotes	PCR
T14968	34642-34650	NN	denotes	reaction
T14969	34655-34665	VBG	denotes	containing
T14970	34666-34681	NN	denotes	oligonucleotide
T14971	34690-34691	CD	denotes	1
T14972	34682-34689	NNS	denotes	primers
T14973	34691-34693	,	denotes	,
T14974	34693-34694	CD	denotes	2
T14975	34694-34696	,	denotes	,
T14976	34696-34699	CC	denotes	and
T14977	34700-34701	CD	denotes	3
T14978	34702-34703	-LRB-	denotes	(
T14979	34710-34712	NN	denotes	1A
T14980	34703-34709	NN	denotes	Figure
T14981	34712-34713	-RRB-	denotes	)
T14982	34714-34718	WDT	denotes	that
T14983	34719-34726	VBP	denotes	amplify
T14984	34727-34730	DT	denotes	the
T14985	34752-34759	NNS	denotes	alleles
T14986	34731-34735	JJ	denotes	wild
T14987	34736-34740	NN	denotes	type
T14988	34735-34736	HYPH	denotes	-
T14989	34741-34744	CC	denotes	and
T14990	34745-34751	NN	denotes	mutant
T14991	34759-34760	.	denotes	.
T14992	34760-34911	sentence	denotes	After 30 cycles of amplification, 1 μl of each PCR reaction was used in a second round of PCR amplification with a new set of oligonucleotide primers.
T14993	34761-34766	IN	denotes	After
T14994	34825-34829	VBN	denotes	used
T14995	34767-34769	CD	denotes	30
T14996	34770-34776	NNS	denotes	cycles
T14997	34777-34779	IN	denotes	of
T14998	34780-34793	NN	denotes	amplification
T14999	34793-34795	,	denotes	,
T15000	34795-34796	CD	denotes	1
T15001	34797-34799	NN	denotes	μl
T15002	34800-34802	IN	denotes	of
T15003	34803-34807	DT	denotes	each
T15004	34812-34820	NN	denotes	reaction
T15005	34808-34811	NN	denotes	PCR
T15006	34821-34824	VBD	denotes	was
T15007	34830-34832	IN	denotes	in
T15008	34833-34834	DT	denotes	a
T15009	34842-34847	NN	denotes	round
T15010	34835-34841	JJ	denotes	second
T15011	34848-34850	IN	denotes	of
T15012	34851-34854	NN	denotes	PCR
T15013	34855-34868	NN	denotes	amplification
T15014	34869-34873	IN	denotes	with
T15015	34874-34875	DT	denotes	a
T15016	34880-34883	NN	denotes	set
T15017	34876-34879	JJ	denotes	new
T15018	34884-34886	IN	denotes	of
T15019	34887-34902	NN	denotes	oligonucleotide
T15020	34903-34910	NNS	denotes	primers
T15021	34910-34911	.	denotes	.
T15022	34911-35077	sentence	denotes	After 30 cycles of amplification, PCR reactions were run onto an agarose gel, which revealed the presence of the wild-type (150 bp) and recombinant (200 bp) alleles.
T15023	34912-34917	IN	denotes	After
T15024	34965-34968	VBN	denotes	run
T15025	34918-34920	CD	denotes	30
T15026	34921-34927	NNS	denotes	cycles
T15027	34928-34930	IN	denotes	of
T15028	34931-34944	NN	denotes	amplification
T15029	34944-34946	,	denotes	,
T15030	34946-34949	NN	denotes	PCR
T15031	34950-34959	NNS	denotes	reactions
T15032	34960-34964	VBD	denotes	were
T15033	34969-34973	IN	denotes	onto
T15034	34974-34976	DT	denotes	an
T15035	34985-34988	NN	denotes	gel
T15036	34977-34984	NN	denotes	agarose
T15037	34988-34990	,	denotes	,
T15038	34990-34995	WDT	denotes	which
T15039	34996-35004	VBD	denotes	revealed
T15040	35005-35008	DT	denotes	the
T15041	35009-35017	NN	denotes	presence
T15042	35018-35020	IN	denotes	of
T15043	35021-35024	DT	denotes	the
T15044	35069-35076	NNS	denotes	alleles
T15045	35025-35029	JJ	denotes	wild
T15046	35030-35034	NN	denotes	type
T15047	35029-35030	HYPH	denotes	-
T15048	35035-35036	-LRB-	denotes	(
T15049	35040-35042	NN	denotes	bp
T15050	35036-35039	CD	denotes	150
T15051	35042-35043	-RRB-	denotes	)
T15052	35044-35047	CC	denotes	and
T15053	35048-35059	NN	denotes	recombinant
T15054	35060-35061	-LRB-	denotes	(
T15055	35065-35067	NN	denotes	bp
T15056	35061-35064	CD	denotes	200
T15057	35067-35068	-RRB-	denotes	)
T15058	35076-35077	.	denotes	.
T15693	35079-35086	NN	denotes	P150CAF
T15694	35089-35098	NN	denotes	depletion
T15695	35086-35087	HYPH	denotes	-
T15696	35087-35088	CD	denotes	1
T15697	35099-35101	IN	denotes	by
T15698	35102-35106	NN	denotes	RNAi
T15699	35106-35107	.	denotes	.
T15700	35107-35251	sentence	denotes	The RNAi plasmid vector that we used in this study contains the mouse H1 promoter and a puromycin selection gene (SB and MG, unpublished data).
T15701	35108-35111	DT	denotes	The
T15702	35125-35131	NN	denotes	vector
T15703	35112-35116	NN	denotes	RNAi
T15704	35117-35124	NN	denotes	plasmid
T15705	35159-35167	VBZ	denotes	contains
T15706	35132-35136	WDT	denotes	that
T15707	35140-35144	VBD	denotes	used
T15708	35137-35139	PRP	denotes	we
T15709	35145-35147	IN	denotes	in
T15710	35148-35152	DT	denotes	this
T15711	35153-35158	NN	denotes	study
T15712	35168-35171	DT	denotes	the
T15713	35181-35189	NN	denotes	promoter
T15714	35172-35177	NN	denotes	mouse
T15715	35178-35180	NN	denotes	H1
T15716	35190-35193	CC	denotes	and
T15717	35194-35195	DT	denotes	a
T15718	35216-35220	NN	denotes	gene
T15719	35196-35205	NN	denotes	puromycin
T15720	35206-35215	NN	denotes	selection
T15721	35221-35222	-LRB-	denotes	(
T15722	35245-35249	NNS	denotes	data
T15723	35222-35224	NN	denotes	SB
T15724	35225-35228	CC	denotes	and
T15725	35229-35231	NN	denotes	MG
T15726	35231-35233	,	denotes	,
T15727	35233-35244	JJ	denotes	unpublished
T15728	35249-35250	-RRB-	denotes	)
T15729	35250-35251	.	denotes	.
T15730	35251-35517	sentence	denotes	We characterized in detail the properties of this new vector using a GFP target gene and FACS: Extinction of target genes in ES cells is highly efficient in 70% of the cells, of intermediate efficiency in 15%, and inefficient in the remaining 15% of cells analysis.
T15731	35252-35254	PRP	denotes	We
T15732	35255-35268	VBD	denotes	characterized
T15733	35386-35388	VBZ	denotes	is
T15734	35269-35271	IN	denotes	in
T15735	35272-35278	NN	denotes	detail
T15736	35279-35282	DT	denotes	the
T15737	35283-35293	NNS	denotes	properties
T15738	35294-35296	IN	denotes	of
T15739	35297-35301	DT	denotes	this
T15740	35306-35312	NN	denotes	vector
T15741	35302-35305	JJ	denotes	new
T15742	35313-35318	VBG	denotes	using
T15743	35319-35320	DT	denotes	a
T15744	35332-35336	NN	denotes	gene
T15745	35321-35324	NN	denotes	GFP
T15746	35325-35331	NN	denotes	target
T15747	35337-35340	CC	denotes	and
T15748	35341-35345	NN	denotes	FACS
T15749	35345-35347	:	denotes	:
T15750	35347-35357	NN	denotes	Extinction
T15751	35358-35360	IN	denotes	of
T15752	35361-35367	NN	denotes	target
T15753	35368-35373	NNS	denotes	genes
T15754	35374-35376	IN	denotes	in
T15755	35377-35379	NN	denotes	ES
T15756	35380-35385	NNS	denotes	cells
T15757	35389-35395	RB	denotes	highly
T15758	35396-35405	JJ	denotes	efficient
T15759	35406-35408	IN	denotes	in
T15760	35409-35411	CD	denotes	70
T15761	35411-35412	NN	denotes	%
T15762	35413-35415	IN	denotes	of
T15763	35416-35419	DT	denotes	the
T15764	35420-35425	NNS	denotes	cells
T15765	35425-35427	,	denotes	,
T15766	35427-35429	IN	denotes	of
T15767	35430-35442	JJ	denotes	intermediate
T15768	35443-35453	NN	denotes	efficiency
T15769	35454-35456	IN	denotes	in
T15770	35457-35459	CD	denotes	15
T15771	35459-35460	NN	denotes	%
T15772	35460-35462	,	denotes	,
T15773	35462-35465	CC	denotes	and
T15774	35466-35477	JJ	denotes	inefficient
T15775	35478-35480	IN	denotes	in
T15776	35481-35484	DT	denotes	the
T15777	35497-35498	NN	denotes	%
T15778	35485-35494	VBG	denotes	remaining
T15779	35495-35497	CD	denotes	15
T15780	35499-35501	IN	denotes	of
T15781	35502-35507	NNS	denotes	cells
T15782	35508-35516	NN	denotes	analysis
T15783	35516-35517	.	denotes	.
T15784	35517-35615	sentence	denotes	The sequence of p150CAF-1 siRNA (short interfering RNA) duplex [16] was cloned into this plasmid.
T15785	35518-35521	DT	denotes	The
T15786	35522-35530	NN	denotes	sequence
T15787	35590-35596	VBN	denotes	cloned
T15788	35531-35533	IN	denotes	of
T15789	35534-35541	NN	denotes	p150CAF
T15790	35574-35580	NN	denotes	duplex
T15791	35541-35542	HYPH	denotes	-
T15792	35542-35543	CD	denotes	1
T15793	35544-35549	NN	denotes	siRNA
T15794	35550-35551	-LRB-	denotes	(
T15795	35551-35556	JJ	denotes	short
T15796	35569-35572	NN	denotes	RNA
T15797	35557-35568	VBG	denotes	interfering
T15798	35572-35573	-RRB-	denotes	)
T15799	35581-35582	-LRB-	denotes	[
T15800	35582-35584	CD	denotes	16
T15801	35584-35585	-RRB-	denotes	]
T15802	35586-35589	VBD	denotes	was
T15803	35597-35601	IN	denotes	into
T15804	35602-35606	DT	denotes	this
T15805	35607-35614	NN	denotes	plasmid
T15806	35614-35615	.	denotes	.
T15807	35615-35686	sentence	denotes	The control vector expressed a siRNA that targets GFP RNA degradation.
T15808	35616-35619	DT	denotes	The
T15809	35628-35634	NN	denotes	vector
T15810	35620-35627	NN	denotes	control
T15811	35635-35644	VBD	denotes	expressed
T15812	35645-35646	DT	denotes	a
T15813	35647-35652	NN	denotes	siRNA
T15814	35653-35657	WDT	denotes	that
T15815	35658-35665	VBZ	denotes	targets
T15816	35666-35669	NN	denotes	GFP
T15817	35674-35685	NN	denotes	degradation
T15818	35670-35673	NN	denotes	RNA
T15819	35685-35686	.	denotes	.
T15820	35686-35846	sentence	denotes	ES cells were transfected by electroporation with the RNA plasmid vector, seeded onto gelatin-coated slides, and cultured for 24 h in the absence of selection.
T15821	35687-35689	NN	denotes	ES
T15822	35690-35695	NNS	denotes	cells
T15823	35701-35712	VBN	denotes	transfected
T15824	35696-35700	VBD	denotes	were
T15825	35713-35715	IN	denotes	by
T15826	35716-35731	NN	denotes	electroporation
T15827	35732-35736	IN	denotes	with
T15828	35737-35740	DT	denotes	the
T15829	35753-35759	NN	denotes	vector
T15830	35741-35744	NN	denotes	RNA
T15831	35745-35752	NN	denotes	plasmid
T15832	35759-35761	,	denotes	,
T15833	35761-35767	VBN	denotes	seeded
T15834	35768-35772	IN	denotes	onto
T15835	35773-35780	NN	denotes	gelatin
T15836	35781-35787	VBN	denotes	coated
T15837	35780-35781	HYPH	denotes	-
T15838	35788-35794	NNS	denotes	slides
T15839	35794-35796	,	denotes	,
T15840	35796-35799	CC	denotes	and
T15841	35800-35808	VBN	denotes	cultured
T15842	35809-35812	IN	denotes	for
T15843	35813-35815	CD	denotes	24
T15844	35816-35817	NN	denotes	h
T15845	35818-35820	IN	denotes	in
T15846	35821-35824	DT	denotes	the
T15847	35825-35832	NN	denotes	absence
T15848	35833-35835	IN	denotes	of
T15849	35836-35845	NN	denotes	selection
T15850	35845-35846	.	denotes	.
T15851	35846-35953	sentence	denotes	Puromycin (2 μg/ml) was added to the culture medium and cells were cultured for an additional 48-h period.
T15852	35847-35856	NN	denotes	Puromycin
T15853	35871-35876	VBN	denotes	added
T15854	35857-35858	-LRB-	denotes	(
T15855	35860-35862	NN	denotes	μg
T15856	35858-35859	CD	denotes	2
T15857	35862-35863	SYM	denotes	/
T15858	35863-35865	NN	denotes	ml
T15859	35865-35866	-RRB-	denotes	)
T15860	35867-35870	VBD	denotes	was
T15861	35877-35879	IN	denotes	to
T15862	35880-35883	DT	denotes	the
T15863	35892-35898	NN	denotes	medium
T15864	35884-35891	NN	denotes	culture
T15865	35899-35902	CC	denotes	and
T15866	35903-35908	NNS	denotes	cells
T15867	35914-35922	VBN	denotes	cultured
T15868	35909-35913	VBD	denotes	were
T15869	35923-35926	IN	denotes	for
T15870	35927-35929	DT	denotes	an
T15871	35946-35952	NN	denotes	period
T15872	35930-35940	JJ	denotes	additional
T15873	35941-35943	CD	denotes	48
T15874	35944-35945	NN	denotes	h
T15875	35943-35944	HYPH	denotes	-
T15876	35952-35953	.	denotes	.
T15877	35953-36078	sentence	denotes	Using the p150CAF-1 RNAi vector, IF microscopy quantification reveals a complete CAF-1 depletion in most cells (Figure S1B).
T15878	35954-35959	VBG	denotes	Using
T15879	36016-36023	VBZ	denotes	reveals
T15880	35960-35963	DT	denotes	the
T15881	35979-35985	NN	denotes	vector
T15882	35964-35971	NN	denotes	p150CAF
T15883	35971-35972	HYPH	denotes	-
T15884	35972-35973	CD	denotes	1
T15885	35974-35978	NN	denotes	RNAi
T15886	35985-35987	,	denotes	,
T15887	35987-35989	NN	denotes	IF
T15888	35990-36000	NN	denotes	microscopy
T15889	36001-36015	NN	denotes	quantification
T15890	36024-36025	DT	denotes	a
T15891	36041-36050	NN	denotes	depletion
T15892	36026-36034	JJ	denotes	complete
T15893	36035-36038	NN	denotes	CAF
T15894	36038-36039	HYPH	denotes	-
T15895	36039-36040	CD	denotes	1
T15896	36051-36053	IN	denotes	in
T15897	36054-36058	JJS	denotes	most
T15898	36059-36064	NNS	denotes	cells
T15899	36065-36066	-LRB-	denotes	(
T15900	36073-36076	NN	denotes	S1B
T15901	36066-36072	NN	denotes	Figure
T15902	36076-36077	-RRB-	denotes	)
T15903	36077-36078	.	denotes	.
T15904	36078-36223	sentence	denotes	Western blot analysis (Figure S1A) reveals residual p150CAF-1 expression, as expected from the 15% of cells that do not inhibit the target gene.
T15905	36079-36086	NNP	denotes	Western
T15906	36087-36091	NN	denotes	blot
T15907	36092-36100	NN	denotes	analysis
T15908	36114-36121	VBZ	denotes	reveals
T15909	36101-36102	-LRB-	denotes	(
T15910	36109-36112	NN	denotes	S1A
T15911	36102-36108	NN	denotes	Figure
T15912	36112-36113	-RRB-	denotes	)
T15913	36122-36130	JJ	denotes	residual
T15914	36141-36151	NN	denotes	expression
T15915	36131-36138	NN	denotes	p150CAF
T15916	36138-36139	HYPH	denotes	-
T15917	36139-36140	CD	denotes	1
T15918	36151-36153	,	denotes	,
T15919	36153-36155	IN	denotes	as
T15920	36156-36164	VBN	denotes	expected
T15921	36165-36169	IN	denotes	from
T15922	36170-36173	DT	denotes	the
T15923	36176-36177	NN	denotes	%
T15924	36174-36176	CD	denotes	15
T15925	36178-36180	IN	denotes	of
T15926	36181-36186	NNS	denotes	cells
T15927	36187-36191	WDT	denotes	that
T15928	36199-36206	VB	denotes	inhibit
T15929	36192-36194	VBP	denotes	do
T15930	36195-36198	RB	denotes	not
T15931	36207-36210	DT	denotes	the
T15932	36218-36222	NN	denotes	gene
T15933	36211-36217	NN	denotes	target
T15934	36222-36223	.	denotes	.
T15935	36223-36429	sentence	denotes	MEFs (passage 3, 90% confluence) were transfected during 4 h with the RNAi plasmid vector using Lipofectamine 2000 (Invitrogene, Carlsbad, California, United States) according to manufacturer's conditions.
T15936	36224-36228	NNS	denotes	MEFs
T15937	36262-36273	VBN	denotes	transfected
T15938	36229-36230	-LRB-	denotes	(
T15939	36230-36237	NN	denotes	passage
T15940	36238-36239	CD	denotes	3
T15941	36239-36241	,	denotes	,
T15942	36241-36243	CD	denotes	90
T15943	36243-36244	NN	denotes	%
T15944	36245-36255	NN	denotes	confluence
T15945	36255-36256	-RRB-	denotes	)
T15946	36257-36261	VBD	denotes	were
T15947	36274-36280	IN	denotes	during
T15948	36281-36282	CD	denotes	4
T15949	36283-36284	NN	denotes	h
T15950	36285-36289	IN	denotes	with
T15951	36290-36293	DT	denotes	the
T15952	36307-36313	NN	denotes	vector
T15953	36294-36298	NN	denotes	RNAi
T15954	36299-36306	NN	denotes	plasmid
T15955	36314-36319	VBG	denotes	using
T15956	36320-36333	NNP	denotes	Lipofectamine
T15957	36334-36338	CD	denotes	2000
T15958	36339-36340	-LRB-	denotes	(
T15959	36340-36351	NNP	denotes	Invitrogene
T15960	36351-36353	,	denotes	,
T15961	36353-36361	NNP	denotes	Carlsbad
T15962	36361-36363	,	denotes	,
T15963	36363-36373	NNP	denotes	California
T15964	36373-36375	,	denotes	,
T15965	36375-36381	NNP	denotes	United
T15966	36382-36388	NNP	denotes	States
T15967	36388-36389	-RRB-	denotes	)
T15968	36390-36399	VBG	denotes	according
T15969	36400-36402	IN	denotes	to
T15970	36403-36415	NN	denotes	manufacturer
T15971	36418-36428	NNS	denotes	conditions
T15972	36415-36417	POS	denotes	's
T15973	36428-36429	.	denotes	.
T15974	36429-36529	sentence	denotes	Cells were trypsinized, plated at 1/3 dilution, and cultured for 48 h in the presence of puromycin.
T15975	36430-36435	NNS	denotes	Cells
T15976	36441-36452	VBN	denotes	trypsinized
T15977	36436-36440	VBD	denotes	were
T15978	36452-36454	,	denotes	,
T15979	36454-36460	VBN	denotes	plated
T15980	36461-36463	IN	denotes	at
T15981	36464-36465	CD	denotes	1
T15982	36466-36467	CD	denotes	3
T15983	36465-36466	SYM	denotes	/
T15984	36468-36476	NN	denotes	dilution
T15985	36476-36478	,	denotes	,
T15986	36478-36481	CC	denotes	and
T15987	36482-36490	VBN	denotes	cultured
T15988	36491-36494	IN	denotes	for
T15989	36495-36497	CD	denotes	48
T15990	36498-36499	NN	denotes	h
T15991	36500-36502	IN	denotes	in
T15992	36503-36506	DT	denotes	the
T15993	36507-36515	NN	denotes	presence
T15994	36516-36518	IN	denotes	of
T15995	36519-36528	NN	denotes	puromycin
T15996	36528-36529	.	denotes	.
T16173	36531-36535	NN	denotes	Cell
T16174	36536-36541	NNS	denotes	lines
T16175	36541-36542	.	denotes	.
T16176	36542-36613	sentence	denotes	AT-1 ES cells [42] (a gift of M. Vernet) were used for gene targeting.
T16177	36543-36545	NN	denotes	AT
T16178	36551-36556	NNS	denotes	cells
T16179	36545-36546	HYPH	denotes	-
T16180	36546-36547	CD	denotes	1
T16181	36548-36550	NN	denotes	ES
T16182	36589-36593	VBN	denotes	used
T16183	36557-36558	-LRB-	denotes	[
T16184	36558-36560	CD	denotes	42
T16185	36560-36561	-RRB-	denotes	]
T16186	36562-36563	-LRB-	denotes	(
T16187	36565-36569	NN	denotes	gift
T16188	36563-36564	DT	denotes	a
T16189	36570-36572	IN	denotes	of
T16190	36573-36575	NNP	denotes	M.
T16191	36576-36582	NNP	denotes	Vernet
T16192	36582-36583	-RRB-	denotes	)
T16193	36584-36588	VBD	denotes	were
T16194	36594-36597	IN	denotes	for
T16195	36598-36602	NN	denotes	gene
T16196	36603-36612	NN	denotes	targeting
T16197	36612-36613	.	denotes	.
T16198	36613-36662	sentence	denotes	LTM7 ES cells were used in all RNAi experiments.
T16199	36614-36618	NN	denotes	LTM7
T16200	36622-36627	NNS	denotes	cells
T16201	36619-36621	NN	denotes	ES
T16202	36633-36637	VBN	denotes	used
T16203	36628-36632	VBD	denotes	were
T16204	36638-36640	IN	denotes	in
T16205	36641-36644	DT	denotes	all
T16206	36650-36661	NNS	denotes	experiments
T16207	36645-36649	NN	denotes	RNAi
T16208	36661-36662	.	denotes	.
T16209	36662-36745	sentence	denotes	We derived this cell line from (C57BL/6 × 129) F1 females bred with C3H/HeJ males.
T16210	36663-36665	PRP	denotes	We
T16211	36666-36673	VBD	denotes	derived
T16212	36674-36678	DT	denotes	this
T16213	36684-36688	NN	denotes	line
T16214	36679-36683	NN	denotes	cell
T16215	36689-36693	IN	denotes	from
T16216	36694-36695	-LRB-	denotes	(
T16217	36695-36700	NN	denotes	C57BL
T16218	36713-36720	NNS	denotes	females
T16219	36700-36701	HYPH	denotes	/
T16220	36701-36702	CD	denotes	6
T16221	36703-36704	SYM	denotes	×
T16222	36705-36708	CD	denotes	129
T16223	36708-36709	-RRB-	denotes	)
T16224	36710-36712	NN	denotes	F1
T16225	36721-36725	VBN	denotes	bred
T16226	36726-36730	IN	denotes	with
T16227	36731-36734	NN	denotes	C3H
T16228	36735-36738	NN	denotes	HeJ
T16229	36734-36735	HYPH	denotes	/
T16230	36739-36744	NNS	denotes	males
T16231	36744-36745	.	denotes	.
T16232	36745-36805	sentence	denotes	LTM7 are XX ES cells, competent for germ line transmission.
T16233	36746-36750	NN	denotes	LTM7
T16234	36751-36754	VBP	denotes	are
T16235	36755-36757	NN	denotes	XX
T16236	36761-36766	NNS	denotes	cells
T16237	36758-36760	NN	denotes	ES
T16238	36766-36768	,	denotes	,
T16239	36768-36777	JJ	denotes	competent
T16240	36778-36781	IN	denotes	for
T16241	36782-36786	NN	denotes	germ
T16242	36787-36791	NN	denotes	line
T16243	36792-36804	NN	denotes	transmission
T16244	36804-36805	.	denotes	.
T16245	36805-36870	sentence	denotes	Primary MEFs were derived from E13 embryos as described in [43].
T16246	36806-36813	JJ	denotes	Primary
T16247	36814-36818	NNS	denotes	MEFs
T16248	36824-36831	VBN	denotes	derived
T16249	36819-36823	VBD	denotes	were
T16250	36832-36836	IN	denotes	from
T16251	36837-36840	NN	denotes	E13
T16252	36841-36848	NNS	denotes	embryos
T16253	36849-36851	IN	denotes	as
T16254	36852-36861	VBN	denotes	described
T16255	36862-36864	IN	denotes	in
T16256	36865-36866	-LRB-	denotes	[
T16257	36866-36868	CD	denotes	43
T16258	36868-36869	-RRB-	denotes	]
T16259	36869-36870	.	denotes	.
T16587	36872-36890	NN	denotes	Immunofluorescence
T16588	36890-36891	.	denotes	.
T16589	36891-37016	sentence	denotes	Cells were fixed for 20 min in PBS with 4% paraformaldehyde, and immunodetection was performed as previously described [44].
T16590	36892-36897	NNS	denotes	Cells
T16591	36903-36908	VBN	denotes	fixed
T16592	36898-36902	VBD	denotes	were
T16593	36909-36912	IN	denotes	for
T16594	36913-36915	CD	denotes	20
T16595	36916-36919	NN	denotes	min
T16596	36920-36922	IN	denotes	in
T16597	36923-36926	NN	denotes	PBS
T16598	36927-36931	IN	denotes	with
T16599	36932-36933	CD	denotes	4
T16600	36933-36934	NN	denotes	%
T16601	36935-36951	NN	denotes	paraformaldehyde
T16602	36951-36953	,	denotes	,
T16603	36953-36956	CC	denotes	and
T16604	36957-36972	NN	denotes	immunodetection
T16605	36977-36986	VBN	denotes	performed
T16606	36973-36976	VBD	denotes	was
T16607	36987-36989	IN	denotes	as
T16608	37001-37010	VBN	denotes	described
T16609	36990-37000	RB	denotes	previously
T16610	37011-37012	-LRB-	denotes	[
T16611	37012-37014	CD	denotes	44
T16612	37014-37015	-RRB-	denotes	]
T16613	37015-37016	.	denotes	.
T16614	37016-37195	sentence	denotes	E4 embryos were collected and treated with tyrode acid to remove the zona pellucida, deposited onto microscope slides, and processed for immunostaining as described for ES cells.
T16615	37017-37019	NN	denotes	E4
T16616	37020-37027	NNS	denotes	embryos
T16617	37033-37042	VBN	denotes	collected
T16618	37028-37032	VBD	denotes	were
T16619	37043-37046	CC	denotes	and
T16620	37047-37054	VBN	denotes	treated
T16621	37055-37059	IN	denotes	with
T16622	37060-37066	NN	denotes	tyrode
T16623	37067-37071	NN	denotes	acid
T16624	37072-37074	TO	denotes	to
T16625	37075-37081	VB	denotes	remove
T16626	37082-37085	DT	denotes	the
T16627	37091-37100	NN	denotes	pellucida
T16628	37086-37090	NN	denotes	zona
T16629	37100-37102	,	denotes	,
T16630	37102-37111	VBN	denotes	deposited
T16631	37112-37116	IN	denotes	onto
T16632	37117-37127	NN	denotes	microscope
T16633	37128-37134	NNS	denotes	slides
T16634	37134-37136	,	denotes	,
T16635	37136-37139	CC	denotes	and
T16636	37140-37149	VBN	denotes	processed
T16637	37150-37153	IN	denotes	for
T16638	37154-37168	NN	denotes	immunostaining
T16639	37169-37171	IN	denotes	as
T16640	37172-37181	VBN	denotes	described
T16641	37182-37185	IN	denotes	for
T16642	37186-37188	NN	denotes	ES
T16643	37189-37194	NNS	denotes	cells
T16644	37194-37195	.	denotes	.
T16645	37195-37479	sentence	denotes	Antibodies anti-HP1α (2HP1H5, Euromedex, France), anti-H4K20me3 (Abcam, Cambridge, United Kingdom), anti-BrdU (DakoCytomation, Glostrup, Denmark), anti-PCNA (DakoCytomation), and anti-PML (Upstate Biotechnology, Lake Placid, New York, United States) were all used at 1/1000 dilution.
T16646	37196-37206	NNS	denotes	Antibodies
T16647	37455-37459	VBN	denotes	used
T16648	37207-37216	JJ	denotes	anti-HP1α
T16649	37217-37218	-LRB-	denotes	(
T16650	37218-37224	NN	denotes	2HP1H5
T16651	37224-37226	,	denotes	,
T16652	37226-37235	NNP	denotes	Euromedex
T16653	37235-37237	,	denotes	,
T16654	37237-37243	NNP	denotes	France
T16655	37243-37244	-RRB-	denotes	)
T16656	37244-37246	,	denotes	,
T16657	37246-37259	JJ	denotes	anti-H4K20me3
T16658	37260-37261	-LRB-	denotes	(
T16659	37261-37266	NNP	denotes	Abcam
T16660	37266-37268	,	denotes	,
T16661	37268-37277	NNP	denotes	Cambridge
T16662	37277-37279	,	denotes	,
T16663	37279-37285	NNP	denotes	United
T16664	37286-37293	NNP	denotes	Kingdom
T16665	37293-37294	-RRB-	denotes	)
T16666	37294-37296	,	denotes	,
T16667	37296-37305	JJ	denotes	anti-BrdU
T16668	37306-37307	-LRB-	denotes	(
T16669	37307-37321	NNP	denotes	DakoCytomation
T16670	37321-37323	,	denotes	,
T16671	37323-37331	NNP	denotes	Glostrup
T16672	37331-37333	,	denotes	,
T16673	37333-37340	NNP	denotes	Denmark
T16674	37340-37341	-RRB-	denotes	)
T16675	37341-37343	,	denotes	,
T16676	37343-37352	JJ	denotes	anti-PCNA
T16677	37353-37354	-LRB-	denotes	(
T16678	37354-37368	NNP	denotes	DakoCytomation
T16679	37368-37369	-RRB-	denotes	)
T16680	37369-37371	,	denotes	,
T16681	37371-37374	CC	denotes	and
T16682	37375-37383	JJ	denotes	anti-PML
T16683	37384-37385	-LRB-	denotes	(
T16684	37393-37406	NNP	denotes	Biotechnology
T16685	37385-37392	NNP	denotes	Upstate
T16686	37406-37408	,	denotes	,
T16687	37408-37412	NNP	denotes	Lake
T16688	37413-37419	NNP	denotes	Placid
T16689	37419-37421	,	denotes	,
T16690	37421-37424	NNP	denotes	New
T16691	37425-37429	NNP	denotes	York
T16692	37429-37431	,	denotes	,
T16693	37431-37437	NNP	denotes	United
T16694	37438-37444	NNP	denotes	States
T16695	37444-37445	-RRB-	denotes	)
T16696	37446-37450	VBD	denotes	were
T16697	37451-37454	RB	denotes	all
T16698	37460-37462	IN	denotes	at
T16699	37463-37464	CD	denotes	1
T16700	37470-37478	NN	denotes	dilution
T16701	37464-37465	SYM	denotes	/
T16702	37465-37469	CD	denotes	1000
T16703	37478-37479	.	denotes	.
T16704	37479-37600	sentence	denotes	Antibodies anti-mouse p150CAF1 [16] and anti-H3K9me3 (Upstate Biotechnology) were used at 1/250 and 1/500, respectively.
T16705	37480-37490	NNS	denotes	Antibodies
T16706	37502-37510	NN	denotes	p150CAF1
T16707	37491-37501	JJ	denotes	anti-mouse
T16708	37562-37566	VBN	denotes	used
T16709	37511-37512	-LRB-	denotes	[
T16710	37512-37514	CD	denotes	16
T16711	37514-37515	-RRB-	denotes	]
T16712	37516-37519	CC	denotes	and
T16713	37520-37532	NN	denotes	anti-H3K9me3
T16714	37533-37534	-LRB-	denotes	(
T16715	37542-37555	NNP	denotes	Biotechnology
T16716	37534-37541	NNP	denotes	Upstate
T16717	37555-37556	-RRB-	denotes	)
T16718	37557-37561	VBD	denotes	were
T16719	37567-37569	IN	denotes	at
T16720	37570-37571	CD	denotes	1
T16721	37571-37572	SYM	denotes	/
T16722	37572-37575	CD	denotes	250
T16723	37576-37579	CC	denotes	and
T16724	37580-37581	CD	denotes	1
T16725	37581-37582	SYM	denotes	/
T16726	37582-37585	CD	denotes	500
T16727	37585-37587	,	denotes	,
T16728	37587-37599	RB	denotes	respectively
T16729	37599-37600	.	denotes	.
T16730	37600-37702	sentence	denotes	All secondary antibodies were purchased from Molecular Probes (Sunnyvale, California, United States).
T16731	37601-37604	DT	denotes	All
T16732	37615-37625	NNS	denotes	antibodies
T16733	37605-37614	JJ	denotes	secondary
T16734	37631-37640	VBN	denotes	purchased
T16735	37626-37630	VBD	denotes	were
T16736	37641-37645	IN	denotes	from
T16737	37646-37655	NNP	denotes	Molecular
T16738	37656-37662	NNP	denotes	Probes
T16739	37663-37664	-LRB-	denotes	(
T16740	37664-37673	NNP	denotes	Sunnyvale
T16741	37673-37675	,	denotes	,
T16742	37675-37685	NNP	denotes	California
T16743	37685-37687	,	denotes	,
T16744	37687-37693	NNP	denotes	United
T16745	37694-37700	NNP	denotes	States
T16746	37700-37701	-RRB-	denotes	)
T16747	37701-37702	.	denotes	.
T16924	37704-37711	NNP	denotes	Western
T16925	37712-37716	NN	denotes	blot
T16926	37717-37725	NN	denotes	analysis
T16927	37725-37726	.	denotes	.
T16928	37726-37805	sentence	denotes	Cells were lysed in Laemli buffer and run onto a 4%–12% SDS PAGE gradient gel.
T16929	37727-37732	NNS	denotes	Cells
T16930	37738-37743	VBN	denotes	lysed
T16931	37733-37737	VBD	denotes	were
T16932	37744-37746	IN	denotes	in
T16933	37747-37753	NNP	denotes	Laemli
T16934	37754-37760	NN	denotes	buffer
T16935	37761-37764	CC	denotes	and
T16936	37765-37768	VBP	denotes	run
T16937	37769-37773	IN	denotes	onto
T16938	37774-37775	DT	denotes	a
T16939	37801-37804	NN	denotes	gel
T16940	37776-37777	CD	denotes	4
T16941	37777-37778	NN	denotes	%
T16942	37778-37779	SYM	denotes	–
T16943	37781-37782	NN	denotes	%
T16944	37779-37781	CD	denotes	12
T16945	37783-37786	NN	denotes	SDS
T16946	37787-37791	NN	denotes	PAGE
T16947	37792-37800	NN	denotes	gradient
T16948	37804-37805	.	denotes	.
T16949	37805-37981	sentence	denotes	We used antibodies against p150CAF-1 ([16]; dilution 1/500), HP1α (2G9, Euromedex; 1/500), Histone H3 (Abcam; 1/500), H3K9me3 (Upstate; 1/500) and β-actin (Upstate; 1/40 000).
T16950	37806-37808	PRP	denotes	We
T16951	37809-37813	VBD	denotes	used
T16952	37814-37824	NNS	denotes	antibodies
T16953	37825-37832	IN	denotes	against
T16954	37833-37840	NN	denotes	p150CAF
T16955	37840-37841	HYPH	denotes	-
T16956	37841-37842	CD	denotes	1
T16957	37843-37844	-LRB-	denotes	(
T16958	37850-37858	NN	denotes	dilution
T16959	37844-37845	-LRB-	denotes	[
T16960	37845-37847	CD	denotes	16
T16961	37847-37848	-RRB-	denotes	]
T16962	37848-37849	:	denotes	;
T16963	37859-37860	CD	denotes	1
T16964	37860-37861	SYM	denotes	/
T16965	37861-37864	CD	denotes	500
T16966	37864-37865	-RRB-	denotes	)
T16967	37865-37867	,	denotes	,
T16968	37867-37871	NN	denotes	HP1α
T16969	37872-37873	-LRB-	denotes	(
T16970	37889-37890	CD	denotes	1
T16971	37873-37876	NN	denotes	2G9
T16972	37876-37878	,	denotes	,
T16973	37878-37887	NNP	denotes	Euromedex
T16974	37887-37888	:	denotes	;
T16975	37890-37891	SYM	denotes	/
T16976	37891-37894	CD	denotes	500
T16977	37894-37895	-RRB-	denotes	)
T16978	37895-37897	,	denotes	,
T16979	37897-37904	NN	denotes	Histone
T16980	37905-37907	NN	denotes	H3
T16981	37908-37909	-LRB-	denotes	(
T16982	37916-37917	CD	denotes	1
T16983	37909-37914	NN	denotes	Abcam
T16984	37914-37915	:	denotes	;
T16985	37917-37918	SYM	denotes	/
T16986	37918-37921	CD	denotes	500
T16987	37921-37922	-RRB-	denotes	)
T16988	37922-37924	,	denotes	,
T16989	37924-37931	NN	denotes	H3K9me3
T16990	37932-37933	-LRB-	denotes	(
T16991	37942-37943	CD	denotes	1
T16992	37933-37940	NNP	denotes	Upstate
T16993	37940-37941	:	denotes	;
T16994	37943-37944	SYM	denotes	/
T16995	37944-37947	CD	denotes	500
T16996	37947-37948	-RRB-	denotes	)
T16997	37949-37952	CC	denotes	and
T16998	37953-37954	NN	denotes	β
T16999	37955-37960	NN	denotes	actin
T17000	37954-37955	HYPH	denotes	-
T17001	37961-37962	-LRB-	denotes	(
T17002	37971-37972	CD	denotes	1
T17003	37962-37969	NNP	denotes	Upstate
T17004	37969-37970	:	denotes	;
T17005	37972-37973	SYM	denotes	/
T17006	37976-37979	CD	denotes	000
T17007	37973-37975	CD	denotes	40
T17008	37979-37980	-RRB-	denotes	)
T17009	37980-37981	.	denotes	.
T17171	37983-37986	NN	denotes	DNA
T17172	37987-37991	NN	denotes	FISH
T17173	37991-37992	.	denotes	.
T17174	37992-38031	sentence	denotes	Probes were described previously [20].
T17175	37993-37999	NNS	denotes	Probes
T17176	38005-38014	VBN	denotes	described
T17177	38000-38004	VBD	denotes	were
T17178	38015-38025	RB	denotes	previously
T17179	38026-38027	-LRB-	denotes	[
T17180	38027-38029	CD	denotes	20
T17181	38029-38030	-RRB-	denotes	]
T17182	38030-38031	.	denotes	.
T17183	38031-38193	sentence	denotes	Biotin-16-dUTP or Digoxigenin-11-dUTP (Roche, Basel, Switzerland) labeled probes were generated by nick translation (Roche) and FISH performed as described [20].
T17184	38032-38038	NN	denotes	Biotin
T17185	38042-38046	NN	denotes	dUTP
T17186	38038-38039	HYPH	denotes	-
T17187	38039-38041	CD	denotes	16
T17188	38041-38042	HYPH	denotes	-
T17189	38098-38105	VBN	denotes	labeled
T17190	38047-38049	CC	denotes	or
T17191	38050-38061	NN	denotes	Digoxigenin
T17192	38065-38069	NN	denotes	dUTP
T17193	38061-38062	HYPH	denotes	-
T17194	38062-38064	CD	denotes	11
T17195	38064-38065	HYPH	denotes	-
T17196	38070-38071	-LRB-	denotes	(
T17197	38071-38076	NNP	denotes	Roche
T17198	38076-38078	,	denotes	,
T17199	38078-38083	NNP	denotes	Basel
T17200	38083-38085	,	denotes	,
T17201	38085-38096	NNP	denotes	Switzerland
T17202	38096-38097	-RRB-	denotes	)
T17203	38106-38112	NNS	denotes	probes
T17204	38118-38127	VBN	denotes	generated
T17205	38113-38117	VBD	denotes	were
T17206	38128-38130	IN	denotes	by
T17207	38131-38135	NN	denotes	nick
T17208	38136-38147	NN	denotes	translation
T17209	38148-38149	-LRB-	denotes	(
T17210	38149-38154	NNP	denotes	Roche
T17211	38154-38155	-RRB-	denotes	)
T17212	38156-38159	CC	denotes	and
T17213	38160-38164	NN	denotes	FISH
T17214	38165-38174	VBN	denotes	performed
T17215	38175-38177	IN	denotes	as
T17216	38178-38187	VBN	denotes	described
T17217	38188-38189	-LRB-	denotes	[
T17218	38189-38191	CD	denotes	20
T17219	38191-38192	-RRB-	denotes	]
T17220	38192-38193	.	denotes	.
T17221	38193-38387	sentence	denotes	Image acquisition was performed with the Deltavision RT microscope (100×, 1.4 NA objective), images were deconvoluted, and fluorescence profiles measured along an arbitrary line using SoftWorx.
T17222	38194-38199	NN	denotes	Image
T17223	38200-38211	NN	denotes	acquisition
T17224	38216-38225	VBN	denotes	performed
T17225	38212-38215	VBD	denotes	was
T17226	38226-38230	IN	denotes	with
T17227	38231-38234	DT	denotes	the
T17228	38250-38260	NN	denotes	microscope
T17229	38235-38246	NNP	denotes	Deltavision
T17230	38247-38249	NN	denotes	RT
T17231	38261-38262	-LRB-	denotes	(
T17232	38275-38284	NN	denotes	objective
T17233	38262-38265	CD	denotes	100
T17234	38265-38266	SYM	denotes	×
T17235	38266-38268	,	denotes	,
T17236	38268-38271	CD	denotes	1.4
T17237	38272-38274	NN	denotes	NA
T17238	38284-38285	-RRB-	denotes	)
T17239	38285-38287	,	denotes	,
T17240	38287-38293	NNS	denotes	images
T17241	38299-38311	VBN	denotes	deconvoluted
T17242	38294-38298	VBD	denotes	were
T17243	38311-38313	,	denotes	,
T17244	38313-38316	CC	denotes	and
T17245	38317-38329	NN	denotes	fluorescence
T17246	38330-38338	NNS	denotes	profiles
T17247	38339-38347	VBN	denotes	measured
T17248	38348-38353	IN	denotes	along
T17249	38354-38356	DT	denotes	an
T17250	38367-38371	NN	denotes	line
T17251	38357-38366	JJ	denotes	arbitrary
T17252	38372-38377	VBG	denotes	using
T17253	38378-38386	NNP	denotes	SoftWorx
T17254	38386-38387	.	denotes	.
T17929	38389-38395	NNS	denotes	Nuclei
T17930	38396-38407	NN	denotes	preparation
T17931	38407-38409	,	denotes	,
T17932	38409-38417	NN	denotes	nuclease
T17933	38418-38427	NN	denotes	digestion
T17934	38427-38429	,	denotes	,
T17935	38429-38432	CC	denotes	and
T17936	38433-38444	JJ	denotes	biochemical
T17937	38445-38453	NN	denotes	analysis
T17938	38454-38456	IN	denotes	of
T17939	38457-38466	NN	denotes	chromatin
T17940	38466-38467	.	denotes	.
T17941	38467-38673	sentence	denotes	ES cells were incubated on ice for 10 min in buffer 1 (15 mM Tris-HCl [pH 7.5], 0.3 M sucrose, 60 mM KCl, 15 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA) with 0.15% IGEPAL (Sigma, St. Louis, Missouri, United States).
T17942	38468-38470	NN	denotes	ES
T17943	38471-38476	NNS	denotes	cells
T17944	38482-38491	VBN	denotes	incubated
T17945	38477-38481	VBD	denotes	were
T17946	38492-38494	IN	denotes	on
T17947	38495-38498	NN	denotes	ice
T17948	38499-38502	IN	denotes	for
T17949	38503-38505	CD	denotes	10
T17950	38506-38509	NN	denotes	min
T17951	38510-38512	IN	denotes	in
T17952	38513-38519	NN	denotes	buffer
T17953	38520-38521	CD	denotes	1
T17954	38522-38523	-LRB-	denotes	(
T17955	38534-38537	NN	denotes	HCl
T17956	38523-38525	CD	denotes	15
T17957	38526-38528	NN	denotes	mM
T17958	38529-38533	NN	denotes	Tris
T17959	38533-38534	HYPH	denotes	-
T17960	38538-38539	-LRB-	denotes	[
T17961	38539-38541	NN	denotes	pH
T17962	38542-38545	CD	denotes	7.5
T17963	38545-38546	-RRB-	denotes	]
T17964	38546-38548	,	denotes	,
T17965	38548-38551	CD	denotes	0.3
T17966	38552-38553	NN	denotes	M
T17967	38554-38561	NN	denotes	sucrose
T17968	38561-38563	,	denotes	,
T17969	38563-38565	CD	denotes	60
T17970	38566-38568	NN	denotes	mM
T17971	38569-38572	NN	denotes	KCl
T17972	38572-38574	,	denotes	,
T17973	38574-38576	CD	denotes	15
T17974	38577-38579	NN	denotes	mM
T17975	38580-38584	NN	denotes	NaCl
T17976	38584-38586	,	denotes	,
T17977	38586-38587	CD	denotes	5
T17978	38588-38590	NN	denotes	mM
T17979	38591-38596	NN	denotes	MgCl2
T17980	38596-38598	,	denotes	,
T17981	38598-38601	CD	denotes	0.1
T17982	38602-38604	NN	denotes	mM
T17983	38605-38609	NN	denotes	EGTA
T17984	38609-38610	-RRB-	denotes	)
T17985	38611-38615	IN	denotes	with
T17986	38616-38620	CD	denotes	0.15
T17987	38620-38621	NN	denotes	%
T17988	38622-38628	NN	denotes	IGEPAL
T17989	38629-38630	-LRB-	denotes	(
T17990	38630-38635	NNP	denotes	Sigma
T17991	38635-38637	,	denotes	,
T17992	38637-38640	NNP	denotes	St.
T17993	38641-38646	NNP	denotes	Louis
T17994	38646-38648	,	denotes	,
T17995	38648-38656	NNP	denotes	Missouri
T17996	38656-38658	,	denotes	,
T17997	38658-38664	NNP	denotes	United
T17998	38665-38671	NNP	denotes	States
T17999	38671-38672	-RRB-	denotes	)
T18000	38672-38673	.	denotes	.
T18001	38673-38909	sentence	denotes	Nuclei were purified by centrifugation (10,000 × g for 30 min, at 4 °C) on sucrose cushions (buffer 1 with 1.2 M sucrose) and resuspended in nuclease buffer (50 mM Tris-HCl [pH 7.5], 20 mM NaCl, 0.32 M sucrose, 4 mM MgCl2, 1 mM CaCl2).
T18002	38674-38680	NNS	denotes	Nuclei
T18003	38686-38694	VBN	denotes	purified
T18004	38681-38685	VBD	denotes	were
T18005	38695-38697	IN	denotes	by
T18006	38698-38712	NN	denotes	centrifugation
T18007	38713-38714	-LRB-	denotes	(
T18008	38723-38724	NN	denotes	g
T18009	38714-38720	CD	denotes	10,000
T18010	38721-38722	SYM	denotes	×
T18011	38725-38728	IN	denotes	for
T18012	38729-38731	CD	denotes	30
T18013	38732-38735	NN	denotes	min
T18014	38735-38737	,	denotes	,
T18015	38737-38739	IN	denotes	at
T18016	38740-38741	CD	denotes	4
T18017	38742-38744	NN	denotes	°C
T18018	38744-38745	-RRB-	denotes	)
T18019	38746-38748	IN	denotes	on
T18020	38749-38756	NN	denotes	sucrose
T18021	38757-38765	NNS	denotes	cushions
T18022	38766-38767	-LRB-	denotes	(
T18023	38767-38773	NN	denotes	buffer
T18024	38774-38775	CD	denotes	1
T18025	38776-38780	IN	denotes	with
T18026	38781-38784	CD	denotes	1.2
T18027	38785-38786	NN	denotes	M
T18028	38787-38794	NN	denotes	sucrose
T18029	38794-38795	-RRB-	denotes	)
T18030	38796-38799	CC	denotes	and
T18031	38800-38811	VBN	denotes	resuspended
T18032	38812-38814	IN	denotes	in
T18033	38815-38823	NN	denotes	nuclease
T18034	38824-38830	NN	denotes	buffer
T18035	38831-38832	-LRB-	denotes	(
T18036	38843-38846	NN	denotes	HCl
T18037	38832-38834	CD	denotes	50
T18038	38835-38837	NN	denotes	mM
T18039	38838-38842	NN	denotes	Tris
T18040	38842-38843	HYPH	denotes	-
T18041	38847-38848	-LRB-	denotes	[
T18042	38848-38850	NN	denotes	pH
T18043	38851-38854	CD	denotes	7.5
T18044	38854-38855	-RRB-	denotes	]
T18045	38855-38857	,	denotes	,
T18046	38857-38859	CD	denotes	20
T18047	38860-38862	NN	denotes	mM
T18048	38863-38867	NN	denotes	NaCl
T18049	38867-38869	,	denotes	,
T18050	38869-38873	CD	denotes	0.32
T18051	38874-38875	NN	denotes	M
T18052	38876-38883	NN	denotes	sucrose
T18053	38883-38885	,	denotes	,
T18054	38885-38886	CD	denotes	4
T18055	38887-38889	NN	denotes	mM
T18056	38890-38895	NN	denotes	MgCl2
T18057	38895-38897	,	denotes	,
T18058	38897-38898	CD	denotes	1
T18059	38899-38901	NN	denotes	mM
T18060	38902-38907	NN	denotes	CaCl2
T18061	38907-38908	-RRB-	denotes	)
T18062	38908-38909	.	denotes	.
T18063	38909-39024	sentence	denotes	About 2.106 nuclei were incubated with increasing quantities of MNase (0.04–1.6 units) or DNase I (0.25–16 units).
T18064	38910-38915	IN	denotes	About
T18065	38916-38921	CD	denotes	2.106
T18066	38922-38928	NNS	denotes	nuclei
T18067	38934-38943	VBN	denotes	incubated
T18068	38929-38933	VBD	denotes	were
T18069	38944-38948	IN	denotes	with
T18070	38949-38959	VBG	denotes	increasing
T18071	38960-38970	NNS	denotes	quantities
T18072	38971-38973	IN	denotes	of
T18073	38974-38979	NN	denotes	MNase
T18074	38980-38981	-LRB-	denotes	(
T18075	38990-38995	NNS	denotes	units
T18076	38981-38985	CD	denotes	0.04
T18077	38986-38989	CD	denotes	1.6
T18078	38985-38986	SYM	denotes	–
T18079	38995-38996	-RRB-	denotes	)
T18080	38997-38999	CC	denotes	or
T18081	39000-39005	NN	denotes	DNase
T18082	39006-39007	CD	denotes	I
T18083	39008-39009	-LRB-	denotes	(
T18084	39017-39022	NNS	denotes	units
T18085	39009-39013	CD	denotes	0.25
T18086	39014-39016	CD	denotes	16
T18087	39013-39014	SYM	denotes	–
T18088	39022-39023	-RRB-	denotes	)
T18089	39023-39024	.	denotes	.
T18090	39024-39092	sentence	denotes	Digestion time at 37 °C was 10 min for MNase and 2 min for DNase I.
T18091	39025-39034	NN	denotes	Digestion
T18092	39035-39039	NN	denotes	time
T18093	39049-39052	VBD	denotes	was
T18094	39040-39042	IN	denotes	at
T18095	39043-39045	CD	denotes	37
T18096	39046-39048	NN	denotes	°C
T18097	39053-39055	CD	denotes	10
T18098	39056-39059	NN	denotes	min
T18099	39060-39063	IN	denotes	for
T18100	39064-39069	NN	denotes	MNase
T18101	39070-39073	CC	denotes	and
T18102	39074-39075	CD	denotes	2
T18103	39076-39079	NN	denotes	min
T18104	39080-39083	IN	denotes	for
T18105	39084-39089	NN	denotes	DNase
T18106	39090-39091	CD	denotes	I
T18107	39091-39092	.	denotes	.
T18108	39092-39155	sentence	denotes	Digestions were stopped by adding SDS to 1% and EDTA to 50 mM.
T18109	39093-39103	NNS	denotes	Digestions
T18110	39109-39116	VBN	denotes	stopped
T18111	39104-39108	VBD	denotes	were
T18112	39117-39119	IN	denotes	by
T18113	39120-39126	VBG	denotes	adding
T18114	39127-39130	NN	denotes	SDS
T18115	39131-39133	IN	denotes	to
T18116	39134-39135	CD	denotes	1
T18117	39135-39136	NN	denotes	%
T18118	39137-39140	CC	denotes	and
T18119	39141-39145	NN	denotes	EDTA
T18120	39146-39148	IN	denotes	to
T18121	39149-39151	CD	denotes	50
T18122	39152-39154	NN	denotes	mM
T18123	39154-39155	.	denotes	.
T18124	39155-39272	sentence	denotes	DNAs were prepared by proteinase K digestion followed by phenol-chloroform extraction and isopropanol precipitation.
T18125	39156-39160	NNS	denotes	DNAs
T18126	39166-39174	VBN	denotes	prepared
T18127	39161-39165	VBD	denotes	were
T18128	39175-39177	IN	denotes	by
T18129	39178-39188	NN	denotes	proteinase
T18130	39189-39190	NN	denotes	K
T18131	39191-39200	NN	denotes	digestion
T18132	39201-39209	VBN	denotes	followed
T18133	39210-39212	IN	denotes	by
T18134	39213-39219	NN	denotes	phenol
T18135	39220-39230	NN	denotes	chloroform
T18136	39219-39220	HYPH	denotes	-
T18137	39231-39241	NN	denotes	extraction
T18138	39242-39245	CC	denotes	and
T18139	39246-39257	NN	denotes	isopropanol
T18140	39258-39271	NN	denotes	precipitation
T18141	39271-39272	.	denotes	.
T18142	39272-39575	sentence	denotes	To test the association of histone H3 with chromatin in control and p150-depleted cells, isolated nuclei were incubated on ice for 30 min in buffer 2 (50 mM Hepes [pH7.9], 20% Glycerol, 3 mM MgCl2, 0.1% IGEPAL, 0.5 mM DTT, 0.5 mM PMSF) supplemented with either 0.1 M, 0.3 M, 0.45 M, 0.7 M, or 1 M NaCl.
T18143	39273-39275	TO	denotes	To
T18144	39276-39280	VB	denotes	test
T18145	39383-39392	VBN	denotes	incubated
T18146	39281-39284	DT	denotes	the
T18147	39285-39296	NN	denotes	association
T18148	39297-39299	IN	denotes	of
T18149	39300-39307	NN	denotes	histone
T18150	39308-39310	NN	denotes	H3
T18151	39311-39315	IN	denotes	with
T18152	39316-39325	NN	denotes	chromatin
T18153	39326-39328	IN	denotes	in
T18154	39329-39336	NN	denotes	control
T18155	39355-39360	NNS	denotes	cells
T18156	39337-39340	CC	denotes	and
T18157	39341-39345	NN	denotes	p150
T18158	39346-39354	VBN	denotes	depleted
T18159	39345-39346	HYPH	denotes	-
T18160	39360-39362	,	denotes	,
T18161	39362-39370	VBN	denotes	isolated
T18162	39371-39377	NNS	denotes	nuclei
T18163	39378-39382	VBD	denotes	were
T18164	39393-39395	IN	denotes	on
T18165	39396-39399	NN	denotes	ice
T18166	39400-39403	IN	denotes	for
T18167	39404-39406	CD	denotes	30
T18168	39407-39410	NN	denotes	min
T18169	39411-39413	IN	denotes	in
T18170	39414-39420	NN	denotes	buffer
T18171	39421-39422	CD	denotes	2
T18172	39423-39424	-LRB-	denotes	(
T18173	39430-39435	NN	denotes	Hepes
T18174	39424-39426	CD	denotes	50
T18175	39427-39429	NN	denotes	mM
T18176	39436-39437	-LRB-	denotes	[
T18177	39437-39442	NN	denotes	pH7.9
T18178	39442-39443	-RRB-	denotes	]
T18179	39443-39445	,	denotes	,
T18180	39445-39447	CD	denotes	20
T18181	39447-39448	NN	denotes	%
T18182	39449-39457	NN	denotes	Glycerol
T18183	39457-39459	,	denotes	,
T18184	39459-39460	CD	denotes	3
T18185	39461-39463	NN	denotes	mM
T18186	39464-39469	NN	denotes	MgCl2
T18187	39469-39471	,	denotes	,
T18188	39471-39474	CD	denotes	0.1
T18189	39474-39475	NN	denotes	%
T18190	39476-39482	NN	denotes	IGEPAL
T18191	39482-39484	,	denotes	,
T18192	39484-39487	CD	denotes	0.5
T18193	39488-39490	NN	denotes	mM
T18194	39491-39494	NN	denotes	DTT
T18195	39494-39496	,	denotes	,
T18196	39496-39499	CD	denotes	0.5
T18197	39500-39502	NN	denotes	mM
T18198	39503-39507	NN	denotes	PMSF
T18199	39507-39508	-RRB-	denotes	)
T18200	39509-39521	VBN	denotes	supplemented
T18201	39522-39526	IN	denotes	with
T18202	39527-39533	CC	denotes	either
T18203	39538-39539	NN	denotes	M
T18204	39534-39537	CD	denotes	0.1
T18205	39570-39574	NN	denotes	NaCl
T18206	39539-39541	,	denotes	,
T18207	39541-39544	CD	denotes	0.3
T18208	39545-39546	NN	denotes	M
T18209	39546-39548	,	denotes	,
T18210	39548-39552	CD	denotes	0.45
T18211	39553-39554	NN	denotes	M
T18212	39554-39556	,	denotes	,
T18213	39556-39559	CD	denotes	0.7
T18214	39560-39561	NN	denotes	M
T18215	39561-39563	,	denotes	,
T18216	39563-39565	CC	denotes	or
T18217	39566-39567	CD	denotes	1
T18218	39568-39569	NN	denotes	M
T18219	39574-39575	.	denotes	.
T18220	39575-39699	sentence	denotes	After centrifugation at 15,000 g, pellets and supernants were analyzed by Western blot using a histone H3 antibody (Abcam).
T18221	39576-39581	IN	denotes	After
T18222	39638-39646	VBN	denotes	analyzed
T18223	39582-39596	NN	denotes	centrifugation
T18224	39597-39599	IN	denotes	at
T18225	39600-39606	CD	denotes	15,000
T18226	39607-39608	NN	denotes	g
T18227	39608-39610	,	denotes	,
T18228	39610-39617	NNS	denotes	pellets
T18229	39618-39621	CC	denotes	and
T18230	39622-39632	NNS	denotes	supernants
T18231	39633-39637	VBD	denotes	were
T18232	39647-39649	IN	denotes	by
T18233	39650-39657	NNP	denotes	Western
T18234	39658-39662	NN	denotes	blot
T18235	39663-39668	VBG	denotes	using
T18236	39669-39670	DT	denotes	a
T18237	39682-39690	NN	denotes	antibody
T18238	39671-39678	NN	denotes	histone
T18239	39679-39681	NN	denotes	H3
T18240	39691-39692	-LRB-	denotes	(
T18241	39692-39697	NNP	denotes	Abcam
T18242	39697-39698	-RRB-	denotes	)
T18243	39698-39699	.	denotes	.
T18631	39701-39705	NN	denotes	ChIP
T18632	39705-39706	.	denotes	.
T18633	39706-39800	sentence	denotes	We prepared native chromatin fragments of two to six nucleosomes in length as described [28].
T18634	39707-39709	PRP	denotes	We
T18635	39710-39718	VBD	denotes	prepared
T18636	39719-39725	JJ	denotes	native
T18637	39736-39745	NNS	denotes	fragments
T18638	39726-39735	NN	denotes	chromatin
T18639	39746-39748	IN	denotes	of
T18640	39749-39752	CD	denotes	two
T18641	39756-39759	CD	denotes	six
T18642	39753-39755	IN	denotes	to
T18643	39760-39771	NNS	denotes	nucleosomes
T18644	39772-39774	IN	denotes	in
T18645	39775-39781	NN	denotes	length
T18646	39782-39784	IN	denotes	as
T18647	39785-39794	VBN	denotes	described
T18648	39795-39796	-LRB-	denotes	[
T18649	39796-39798	CD	denotes	28
T18650	39798-39799	-RRB-	denotes	]
T18651	39799-39800	.	denotes	.
T18652	39800-39880	sentence	denotes	5 μg of chromatin were incubated overnight with 10 μl of commercial antibodies.
T18653	39801-39802	CD	denotes	5
T18654	39803-39805	NN	denotes	μg
T18655	39824-39833	VBN	denotes	incubated
T18656	39806-39808	IN	denotes	of
T18657	39809-39818	NN	denotes	chromatin
T18658	39819-39823	VBD	denotes	were
T18659	39834-39843	RB	denotes	overnight
T18660	39844-39848	IN	denotes	with
T18661	39849-39851	CD	denotes	10
T18662	39852-39854	NN	denotes	μl
T18663	39855-39857	IN	denotes	of
T18664	39858-39868	JJ	denotes	commercial
T18665	39869-39879	NNS	denotes	antibodies
T18666	39879-39880	.	denotes	.
T18667	39880-40035	sentence	denotes	After incubation with protein-G sepharose and three washes, immunoprecipitated DNA was purified, sized onto an agarose gel, and analyzed by Southern blot.
T18668	39881-39886	IN	denotes	After
T18669	39968-39976	VBN	denotes	purified
T18670	39887-39897	NN	denotes	incubation
T18671	39898-39902	IN	denotes	with
T18672	39903-39910	NN	denotes	protein
T18673	39911-39912	NN	denotes	G
T18674	39910-39911	HYPH	denotes	-
T18675	39913-39922	NN	denotes	sepharose
T18676	39923-39926	CC	denotes	and
T18677	39927-39932	CD	denotes	three
T18678	39933-39939	NNS	denotes	washes
T18679	39939-39941	,	denotes	,
T18680	39941-39959	VBN	denotes	immunoprecipitated
T18681	39960-39963	NN	denotes	DNA
T18682	39964-39967	VBD	denotes	was
T18683	39976-39978	,	denotes	,
T18684	39978-39983	VBN	denotes	sized
T18685	39984-39988	IN	denotes	onto
T18686	39989-39991	DT	denotes	an
T18687	40000-40003	NN	denotes	gel
T18688	39992-39999	NN	denotes	agarose
T18689	40003-40005	,	denotes	,
T18690	40005-40008	CC	denotes	and
T18691	40009-40017	VBN	denotes	analyzed
T18692	40018-40020	IN	denotes	by
T18693	40021-40029	NNP	denotes	Southern
T18694	40030-40034	NN	denotes	blot
T18695	40034-40035	.	denotes	.
T18696	40035-40150	sentence	denotes	Hybridization signals were quantified using an Instant Imager (PerkinElmer, Wellesley, California, United States).
T18697	40036-40049	NN	denotes	Hybridization
T18698	40050-40057	NNS	denotes	signals
T18699	40063-40073	VBN	denotes	quantified
T18700	40058-40062	VBD	denotes	were
T18701	40074-40079	VBG	denotes	using
T18702	40080-40082	DT	denotes	an
T18703	40091-40097	NNP	denotes	Imager
T18704	40083-40090	NNP	denotes	Instant
T18705	40098-40099	-LRB-	denotes	(
T18706	40099-40110	NNP	denotes	PerkinElmer
T18707	40110-40112	,	denotes	,
T18708	40112-40121	NNP	denotes	Wellesley
T18709	40121-40123	,	denotes	,
T18710	40123-40133	NNP	denotes	California
T18711	40133-40135	,	denotes	,
T18712	40135-40141	NNP	denotes	United
T18713	40142-40148	NNP	denotes	States
T18714	40148-40149	-RRB-	denotes	)
T18715	40149-40150	.	denotes	.
T18716	40150-40210	sentence	denotes	Antibodies specific for H4K20me3 were purchased from Abcam.
T18717	40151-40161	NNS	denotes	Antibodies
T18718	40189-40198	VBN	denotes	purchased
T18719	40162-40170	JJ	denotes	specific
T18720	40171-40174	IN	denotes	for
T18721	40175-40183	NN	denotes	H4K20me3
T18722	40184-40188	VBD	denotes	were
T18723	40199-40203	IN	denotes	from
T18724	40204-40209	NNP	denotes	Abcam
T18725	40209-40210	.	denotes	.
T18726	40210-40316	sentence	denotes	Antibodies for H3K9me3 were purchased from both Abcam and Upstate Biotechnology and gave similar results.
T18727	40211-40221	NNS	denotes	Antibodies
T18728	40239-40248	VBN	denotes	purchased
T18729	40222-40225	IN	denotes	for
T18730	40226-40233	NN	denotes	H3K9me3
T18731	40234-40238	VBD	denotes	were
T18732	40249-40253	IN	denotes	from
T18733	40254-40258	CC	denotes	both
T18734	40259-40264	NNP	denotes	Abcam
T18735	40265-40268	CC	denotes	and
T18736	40269-40276	NNP	denotes	Upstate
T18737	40277-40290	NNP	denotes	Biotechnology
T18738	40291-40294	CC	denotes	and
T18739	40295-40299	VBD	denotes	gave
T18740	40300-40307	JJ	denotes	similar
T18741	40308-40315	NNS	denotes	results
T18742	40315-40316	.	denotes	.
T18743	40316-40544	sentence	denotes	For Southern blot analysis, we used a 240-bp EcoRI/BamHI fragment from pSAT (major satellite probe) [45–47] and a 360-bp EcoRI/HindIII fragment from R198, corresponding to three copies of the 120-bp minor satellite repeat [49].
T18744	40317-40320	IN	denotes	For
T18745	40348-40352	VBD	denotes	used
T18746	40321-40329	NNP	denotes	Southern
T18747	40330-40334	NN	denotes	blot
T18748	40335-40343	NN	denotes	analysis
T18749	40343-40345	,	denotes	,
T18750	40345-40347	PRP	denotes	we
T18751	40353-40354	DT	denotes	a
T18752	40374-40382	NN	denotes	fragment
T18753	40355-40358	CD	denotes	240
T18754	40359-40361	NN	denotes	bp
T18755	40358-40359	HYPH	denotes	-
T18756	40362-40367	NN	denotes	EcoRI
T18757	40368-40373	NN	denotes	BamHI
T18758	40367-40368	HYPH	denotes	/
T18759	40383-40387	IN	denotes	from
T18760	40388-40392	NN	denotes	pSAT
T18761	40393-40394	-LRB-	denotes	(
T18762	40410-40415	NN	denotes	probe
T18763	40394-40399	JJ	denotes	major
T18764	40400-40409	NN	denotes	satellite
T18765	40415-40416	-RRB-	denotes	)
T18766	40417-40418	-LRB-	denotes	[
T18767	40418-40420	CD	denotes	45
T18768	40420-40421	SYM	denotes	–
T18769	40421-40423	CD	denotes	47
T18770	40423-40424	-RRB-	denotes	]
T18771	40425-40428	CC	denotes	and
T18772	40429-40430	DT	denotes	a
T18773	40452-40460	NN	denotes	fragment
T18774	40431-40434	CD	denotes	360
T18775	40435-40437	NN	denotes	bp
T18776	40434-40435	HYPH	denotes	-
T18777	40438-40443	NN	denotes	EcoRI
T18778	40444-40451	NN	denotes	HindIII
T18779	40443-40444	HYPH	denotes	/
T18780	40461-40465	IN	denotes	from
T18781	40466-40470	NN	denotes	R198
T18782	40470-40472	,	denotes	,
T18783	40472-40485	VBG	denotes	corresponding
T18784	40486-40488	IN	denotes	to
T18785	40489-40494	CD	denotes	three
T18786	40495-40501	NNS	denotes	copies
T18787	40502-40504	IN	denotes	of
T18788	40505-40508	DT	denotes	the
T18789	40532-40538	NN	denotes	repeat
T18790	40509-40512	CD	denotes	120
T18791	40513-40515	NN	denotes	bp
T18792	40512-40513	HYPH	denotes	-
T18793	40516-40521	JJ	denotes	minor
T18794	40522-40531	NN	denotes	satellite
T18795	40539-40540	-LRB-	denotes	[
T18796	40540-40542	CD	denotes	49
T18797	40542-40543	-RRB-	denotes	]
T18798	40543-40544	.	denotes	.
T18799	40544-40603	sentence	denotes	Probes used in FISH experiments were described in [46–48].
T18800	40545-40551	NNS	denotes	Probes
T18801	40582-40591	VBN	denotes	described
T18802	40552-40556	VBN	denotes	used
T18803	40557-40559	IN	denotes	in
T18804	40560-40564	NN	denotes	FISH
T18805	40565-40576	NNS	denotes	experiments
T18806	40577-40581	VBD	denotes	were
T18807	40592-40594	IN	denotes	in
T18808	40595-40596	-LRB-	denotes	[
T18809	40596-40598	CD	denotes	46
T18810	40598-40599	SYM	denotes	–
T18811	40599-40601	CD	denotes	48
T18812	40601-40602	-RRB-	denotes	]
T18813	40602-40603	.	denotes	.
T18814	40603-40653	sentence	denotes	The probe for IAP elements was described in [50].
T18815	40604-40607	DT	denotes	The
T18816	40608-40613	NN	denotes	probe
T18817	40635-40644	VBN	denotes	described
T18818	40614-40617	IN	denotes	for
T18819	40618-40621	NN	denotes	IAP
T18820	40622-40630	NNS	denotes	elements
T18821	40631-40634	VBD	denotes	was
T18822	40645-40647	IN	denotes	in
T18823	40648-40649	-LRB-	denotes	[
T18824	40649-40651	CD	denotes	50
T18825	40651-40652	-RRB-	denotes	]
T18826	40652-40653	.	denotes	.
T807	0-3	NN	denotes	CAF
T8538	11274-11276	CD	denotes	16
R10	T810	T807	punct	-,CAF
R11	T811	T807	nummod	1,CAF
R12	T812	T808	acomp	Essential,Is
R13	T813	T812	prep	for,Essential
R14	T814	T815	compound	Heterochromatin,Organization
R15	T815	T813	pobj	Organization,for
R16	T816	T808	prep	in,Is
R17	T817	T818	amod	Pluripotent,Cells
R18	T818	T816	pobj	Cells,in
R19	T819	T818	amod	Embryonic,Cells
R20	T822	T823	prep	During,are
R21	T824	T825	amod	mammalian,development
R22	T825	T822	pobj	development,During
R23	T826	T823	punct	", ",are
R24	T827	T828	compound	chromatin,dynamics
R25	T828	T823	nsubj	dynamics,are
R26	T829	T828	cc	and,dynamics
R27	T830	T831	amod	epigenetic,marking
R28	T831	T828	conj	marking,dynamics
R29	T832	T823	acomp	important,are
R30	T833	T832	prep	for,important
R31	T834	T835	compound	genome,reprogramming
R32	T835	T833	pobj	reprogramming,for
R33	T836	T823	punct	.,are
R34	T838	T839	amod	Recent,data
R35	T839	T840	nsubj	data,suggest
R36	T841	T842	det	an,role
R37	T842	T840	dobj	role,suggest
R38	T843	T842	amod	important,role
R39	T844	T842	prep	for,role
R40	T845	T846	det	the,machinery
R41	T846	T844	pobj	machinery,for
R42	T847	T848	compound	chromatin,assembly
R43	T848	T846	compound	assembly,machinery
R44	T849	T842	prep	in,role
R45	T850	T851	det	this,process
R46	T851	T849	pobj	process,in
R47	T852	T840	punct	.,suggest
R48	T854	T855	aux	To,analyze
R49	T855	T856	advcl	analyze,generated
R50	T857	T858	det	the,role
R51	T858	T855	dobj	role,analyze
R52	T859	T858	prep	of,role
R53	T860	T861	compound	chromatin,assembly
R54	T861	T862	compound	assembly,factor
R55	T862	T859	pobj	factor,of
R56	T863	T862	nummod	1,factor
R57	T864	T862	punct	(,factor
R58	T865	T862	appos	CAF,factor
R59	T866	T865	punct	-,CAF
R60	T867	T865	nummod	1,CAF
R61	T868	T862	punct	),factor
R62	T869	T855	prep	during,analyze
R63	T870	T871	compound	pre-implantation,development
R64	T871	T869	pobj	development,during
R65	T872	T856	punct	", ",generated
R66	T873	T856	nsubj	we,generated
R67	T874	T875	det	a,line
R68	T875	T856	dobj	line,generated
R69	T876	T875	compound	mouse,line
R70	T877	T875	acl	carrying,line
R71	T878	T879	det	a,mutation
R72	T879	T877	dobj	mutation,carrying
R73	T880	T879	amod	targeted,mutation
R74	T881	T877	prep	in,carrying
R75	T882	T883	det	the,gene
R76	T883	T881	pobj	gene,in
R77	T884	T883	acl	encoding,gene
R78	T885	T886	poss	its,subunit
R79	T886	T884	dobj	subunit,encoding
R80	T887	T886	amod	large,subunit
R81	T888	T886	punct	", ",subunit
R82	T889	T886	appos	p150CAF,subunit
R83	T890	T889	punct	-,p150CAF
R84	T891	T889	nummod	1,p150CAF
R85	T892	T856	punct	.,generated
R86	T894	T895	nsubj	Loss,leads
R87	T896	T894	prep	of,Loss
R88	T897	T896	pobj	p150CAF,of
R89	T898	T897	punct	-,p150CAF
R90	T899	T897	nummod	1,p150CAF
R91	T900	T894	prep	in,Loss
R92	T901	T902	amod	homozygous,mutants
R93	T902	T900	pobj	mutants,in
R94	T903	T895	prep	to,leads
R95	T904	T905	amod	developmental,arrest
R96	T905	T903	pobj	arrest,to
R97	T906	T905	prep	at,arrest
R98	T907	T908	det	the,stage
R99	T908	T906	pobj	stage,at
R100	T909	T910	nummod	16,cell
R101	T910	T908	compound	cell,stage
R102	T911	T910	punct	-,cell
R103	T912	T895	punct	.,leads
R104	T914	T915	nsubj	Absence,results
R105	T916	T914	prep	of,Absence
R106	T917	T916	pobj	p150CAF,of
R107	T918	T917	punct	-,p150CAF
R108	T919	T917	nummod	1,p150CAF
R109	T920	T914	prep	in,Absence
R110	T921	T922	det	these,embryos
R111	T922	T920	pobj	embryos,in
R112	T923	T915	prep	in,results
R113	T924	T925	amod	severe,alterations
R114	T925	T923	pobj	alterations,in
R115	T926	T925	prep	in,alterations
R116	T927	T928	det	the,organization
R117	T928	T926	pobj	organization,in
R118	T929	T928	amod	nuclear,organization
R119	T930	T928	prep	of,organization
R120	T931	T932	amod	constitutive,heterochromatin
R121	T932	T930	pobj	heterochromatin,of
R122	T933	T915	punct	.,results
R123	T935	T936	nsubj	We,provide
R124	T937	T936	dobj	evidence,provide
R125	T938	T939	mark	that,reorganized
R126	T939	T937	acl	reorganized,evidence
R127	T940	T939	prep	in,reorganized
R128	T941	T942	amod	wild,type
R129	T942	T944	compound	type,embryos
R130	T943	T942	punct	-,type
R131	T944	T940	pobj	embryos,in
R132	T945	T939	punct	", ",reorganized
R133	T946	T947	compound	heterochromatin,domains
R134	T947	T939	nsubjpass	domains,reorganized
R135	T948	T939	auxpass	are,reorganized
R136	T949	T939	advmod	extensively,reorganized
R137	T950	T939	prep	between,reorganized
R138	T951	T952	det	the,stages
R139	T952	T950	pobj	stages,between
R140	T953	T954	nummod	two,cell
R141	T954	T952	nmod	cell,stages
R142	T955	T954	punct	-,cell
R143	T956	T954	cc	and,cell
R144	T957	T954	conj	blastocyst,cell
R145	T958	T936	punct	.,provide
R146	T960	T961	prep	In,displays
R147	T962	T963	nmod	p150CAF,embryos
R148	T963	T960	pobj	embryos,In
R149	T964	T962	punct	-,p150CAF
R150	T965	T962	nummod	1,p150CAF
R151	T966	T963	nmod	mutant,embryos
R152	T967	T968	nummod	16,cell
R153	T968	T970	compound	cell,stage
R154	T969	T968	punct	-,cell
R155	T970	T963	compound	stage,embryos
R156	T971	T961	punct	", ",displays
R157	T972	T973	det	the,organization
R158	T973	T961	nsubj	organization,displays
R159	T974	T973	amod	altered,organization
R160	T975	T973	prep	of,organization
R161	T976	T975	pobj	heterochromatin,of
R162	T977	T961	dobj	similarities,displays
R163	T978	T977	prep	to,similarities
R164	T979	T980	det	the,structure
R165	T980	T978	pobj	structure,to
R166	T981	T980	prep	of,structure
R167	T982	T981	pobj	heterochromatin,of
R168	T983	T961	prep	in,displays
R169	T984	T985	quantmod	two,four
R170	T985	T988	nummod	four,cell
R171	T986	T985	punct	-,four
R172	T987	T985	quantmod	to,four
R173	T988	T990	nmod	cell,stage
R174	T989	T988	punct	-,cell
R175	T990	T991	nmod	stage,embryos
R176	T991	T983	pobj	embryos,in
R177	T992	T993	amod	wild,type
R178	T993	T991	compound	type,embryos
R179	T994	T993	punct	-,type
R180	T995	T961	punct	", ",displays
R181	T996	T961	advcl	suggesting,displays
R182	T997	T998	mark	that,required
R183	T998	T996	ccomp	required,suggesting
R184	T999	T998	nsubjpass	CAF,required
R185	T1000	T999	punct	-,CAF
R186	T1001	T999	nummod	1,CAF
R187	T1002	T998	auxpass	is,required
R188	T1003	T998	prep	for,required
R189	T1004	T1005	det	the,maturation
R190	T1005	T1003	pobj	maturation,for
R191	T1006	T1005	prep	of,maturation
R192	T1007	T1006	pobj	heterochromatin,of
R193	T1008	T998	prep	during,required
R194	T1009	T1010	compound	preimplantation,development
R195	T1010	T1008	pobj	development,during
R196	T1011	T961	punct	.,displays
R197	T1013	T1014	prep	In,results
R198	T1015	T1016	amod	embryonic,cells
R199	T1016	T1013	pobj	cells,In
R200	T1017	T1016	compound	stem,cells
R201	T1018	T1014	punct	", ",results
R202	T1019	T1014	nsubj	depletion,results
R203	T1020	T1019	prep	of,depletion
R204	T1021	T1020	pobj	p150CAF,of
R205	T1022	T1021	punct	-,p150CAF
R206	T1023	T1021	nummod	1,p150CAF
R207	T1024	T1019	acl	using,depletion
R208	T1025	T1026	compound	RNA,interference
R209	T1026	T1024	dobj	interference,using
R210	T1027	T1014	prep	in,results
R211	T1028	T1029	det	the,mislocalization
R212	T1029	T1027	pobj	mislocalization,in
R213	T1030	T1029	punct	", ",mislocalization
R214	T1031	T1029	conj	loss,mislocalization
R215	T1032	T1031	prep	of,loss
R216	T1033	T1032	pobj	clustering,of
R217	T1034	T1031	punct	", ",loss
R218	T1035	T1031	cc	and,loss
R219	T1036	T1031	conj	decondensation,loss
R220	T1037	T1029	prep	of,mislocalization
R221	T1038	T1039	amod	pericentric,domains
R222	T1039	T1037	pobj	domains,of
R223	T1040	T1039	compound	heterochromatin,domains
R224	T1041	T1014	punct	.,results
R225	T1043	T1044	advmod	Furthermore,results
R226	T1045	T1044	punct	", ",results
R227	T1046	T1044	nsubj	loss,results
R228	T1047	T1046	prep	of,loss
R229	T1048	T1047	pobj	CAF,of
R230	T1049	T1048	punct	-,CAF
R231	T1050	T1048	nummod	1,CAF
R232	T1051	T1046	prep	in,loss
R233	T1052	T1053	det	these,cells
R234	T1053	T1051	pobj	cells,in
R235	T1054	T1044	prep	in,results
R236	T1055	T1056	det	the,alteration
R237	T1056	T1054	pobj	alteration,in
R238	T1057	T1056	prep	of,alteration
R239	T1058	T1059	amod	epigenetic,methylation
R240	T1059	T1061	compound	methylation,marks
R241	T1060	T1059	compound	histone,methylation
R242	T1061	T1057	pobj	marks,of
R243	T1062	T1056	prep	at,alteration
R244	T1063	T1064	det	the,level
R245	T1064	T1062	pobj	level,at
R246	T1065	T1064	prep	of,level
R247	T1066	T1067	amod	pericentric,heterochromatin
R248	T1067	T1065	pobj	heterochromatin,of
R249	T1068	T1044	punct	.,results
R250	T1070	T1071	det	These,alterations
R251	T1071	T1072	nsubjpass	alterations,found
R252	T1073	T1071	prep	of,alterations
R253	T1074	T1073	pobj	heterochromatin,of
R254	T1075	T1072	auxpass	are,found
R255	T1076	T1072	neg	not,found
R256	T1077	T1072	prep	in,found
R257	T1078	T1079	npadvmod	p150CAF,depleted
R258	T1079	T1083	amod	depleted,fibroblasts
R259	T1080	T1078	punct	-,p150CAF
R260	T1081	T1078	nummod	1,p150CAF
R261	T1082	T1079	punct	-,depleted
R262	T1083	T1077	pobj	fibroblasts,in
R263	T1084	T1083	nmod	mouse,fibroblasts
R264	T1085	T1083	amod	embryonic,fibroblasts
R265	T1086	T1083	punct	", ",fibroblasts
R266	T1087	T1088	dep	which,are
R267	T1088	T1083	relcl	are,fibroblasts
R268	T1089	T1088	attr	cells,are
R269	T1090	T1091	dep	that,committed
R270	T1091	T1089	relcl	committed,cells
R271	T1092	T1091	auxpass	are,committed
R272	T1093	T1091	advmod	already,committed
R273	T1094	T1091	dep	lineage,committed
R274	T1095	T1072	punct	", ",found
R275	T1096	T1072	advcl	suggesting,found
R276	T1097	T1098	mark	that,required
R277	T1098	T1096	ccomp	required,suggesting
R278	T1099	T1098	nsubjpass	CAF,required
R279	T1100	T1099	punct	-,CAF
R280	T1101	T1099	nummod	1,CAF
R281	T1102	T1098	auxpass	is,required
R282	T1103	T1098	advmod	specifically,required
R283	T1104	T1098	prep	for,required
R284	T1105	T1106	compound	heterochromatin,organization
R285	T1106	T1104	pobj	organization,for
R286	T1107	T1098	prep	in,required
R287	T1108	T1109	amod	pluripotent,cells
R288	T1109	T1107	pobj	cells,in
R289	T1110	T1109	amod	embryonic,cells
R290	T1111	T1072	punct	.,found
R291	T1113	T1114	poss	Our,findings
R292	T1114	T1115	nsubj	findings,underline
R293	T1116	T1117	det	the,role
R294	T1117	T1115	dobj	role,underline
R295	T1118	T1117	prep	of,role
R296	T1119	T1120	det	the,machinery
R297	T1120	T1118	pobj	machinery,of
R298	T1121	T1122	compound	chromatin,assembly
R299	T1122	T1120	compound	assembly,machinery
R300	T1123	T1117	prep	in,role
R301	T1124	T1123	pcomp	controlling,in
R302	T1125	T1126	det	the,organization
R303	T1126	T1124	dobj	organization,controlling
R304	T1127	T1126	amod	spatial,organization
R305	T1128	T1126	cc	and,organization
R306	T1129	T1130	amod	epigenetic,marking
R307	T1130	T1126	conj	marking,organization
R308	T1131	T1126	prep	of,organization
R309	T1132	T1133	det	the,genome
R310	T1133	T1131	pobj	genome,of
R311	T1134	T1124	prep	in,controlling
R312	T1135	T1136	amod	early,embryos
R313	T1136	T1134	pobj	embryos,in
R314	T1137	T1136	cc	and,embryos
R315	T1138	T1139	amod	embryonic,cells
R316	T1139	T1136	conj	cells,embryos
R317	T1140	T1139	compound	stem,cells
R318	T1141	T1115	punct	.,underline
R319	T807	T808	nsubj	CAF,Is
R330	T2297	T2298	prep	During,undergoes
R331	T2299	T2300	compound	mouse,development
R332	T2300	T2297	pobj	development,During
R333	T2301	T2300	compound	pre-implantation,development
R334	T2302	T2298	punct	", ",undergoes
R335	T2303	T2304	det	the,genome
R336	T2304	T2298	nsubj	genome,undergoes
R337	T2305	T2306	det	a,series
R338	T2306	T2298	dobj	series,undergoes
R339	T2307	T2306	prep	of,series
R340	T2308	T2309	amod	major,changes
R341	T2309	T2307	pobj	changes,of
R342	T2310	T2309	amod	epigenetic,changes
R343	T2311	T2309	acl	required,changes
R344	T2312	T2311	prep	for,required
R345	T2313	T2314	amod	embryonic,expression
R346	T2314	T2312	pobj	expression,for
R347	T2315	T2314	compound	gene,expression
R348	T2316	T2314	punct	", ",expression
R349	T2317	T2318	det	the,maintenance
R350	T2318	T2314	conj	maintenance,expression
R351	T2319	T2318	prep	of,maintenance
R352	T2320	T2319	pobj	totipotency,of
R353	T2321	T2318	punct	", ",maintenance
R354	T2322	T2318	cc	and,maintenance
R355	T2323	T2324	det	the,events
R356	T2324	T2318	conj	events,maintenance
R357	T2325	T2324	amod	first,events
R358	T2326	T2324	compound	differentiation,events
R359	T2327	T2328	punct	[,2
R360	T2328	T2298	parataxis	2,undergoes
R361	T2329	T2328	nummod	1,2
R362	T2330	T2328	punct	",",2
R363	T2331	T2328	punct	],2
R364	T2332	T2298	punct	.,undergoes
R365	T2334	T2335	mark	While,established
R366	T2335	T2339	advcl	established,point
R367	T2336	T2337	amod	many,studies
R368	T2337	T2335	nsubj	studies,established
R369	T2338	T2335	aux	have,established
R370	T2340	T2341	det	the,importance
R371	T2341	T2335	dobj	importance,established
R372	T2342	T2341	prep	of,importance
R373	T2343	T2344	compound	DNA,methylation
R374	T2344	T2342	pobj	methylation,of
R375	T2345	T2341	prep	in,importance
R376	T2346	T2347	amod	epigenetic,reprogramming
R377	T2347	T2345	pobj	reprogramming,in
R378	T2348	T2339	punct	", ",point
R379	T2349	T2350	amod	recent,data
R380	T2350	T2339	nsubj	data,point
R381	T2351	T2339	prep	to,point
R382	T2352	T2353	det	a,role
R383	T2353	T2351	pobj	role,to
R384	T2354	T2353	amod	crucial,role
R385	T2355	T2353	prep	of,role
R386	T2356	T2355	pobj	chromatin,of
R387	T2357	T2353	prep	in,role
R388	T2358	T2359	det	this,process
R389	T2359	T2357	pobj	process,in
R390	T2360	T2361	punct	[,3
R391	T2361	T2339	parataxis	3,point
R392	T2362	T2363	punct	–,5
R393	T2363	T2361	prep	5,3
R394	T2364	T2361	punct	],3
R395	T2365	T2339	punct	.,point
R396	T2367	T2368	advmod	Yet,know
R397	T2369	T2368	nsubj	we,know
R398	T2370	T2371	advmod	very,little
R399	T2371	T2368	dobj	little,know
R400	T2372	T2371	prep	about,little
R401	T2373	T2374	det	the,role
R402	T2374	T2372	pobj	role,about
R403	T2375	T2374	prep	of,role
R404	T2376	T2377	npadvmod	histone,modifying
R405	T2377	T2378	amod	modifying,enzymes
R406	T2378	T2375	pobj	enzymes,of
R407	T2379	T2378	punct	", ",enzymes
R408	T2380	T2381	compound	chromatin,remodeling
R409	T2381	T2382	compound	remodeling,factors
R410	T2382	T2378	conj	factors,enzymes
R411	T2383	T2382	punct	", ",factors
R412	T2384	T2382	cc	and,factors
R413	T2385	T2386	compound	histone,chaperones
R414	T2386	T2382	conj	chaperones,factors
R415	T2387	T2374	prep	during,role
R416	T2388	T2389	compound	pre-implantation,development
R417	T2389	T2387	pobj	development,during
R418	T2390	T2387	punct	", ",during
R419	T2391	T2387	cc	or,during
R420	T2392	T2387	conj	in,during
R421	T2393	T2394	compound	stem,cells
R422	T2394	T2392	pobj	cells,in
R423	T2395	T2394	acl	derived,cells
R424	T2396	T2395	prep	from,derived
R425	T2397	T2398	amod	early,embryos
R426	T2398	T2396	pobj	embryos,from
R427	T2399	T2400	punct	[,6
R428	T2400	T2368	parataxis	6,know
R429	T2401	T2400	punct	],6
R430	T2402	T2368	punct	.,know
R431	T2404	T2405	det	A,subset
R432	T2405	T2406	nsubj	subset,collaborate
R433	T2407	T2405	prep	of,subset
R434	T2408	T2409	amod	identified,chaperones
R435	T2409	T2407	pobj	chaperones,of
R436	T2410	T2409	compound	histone,chaperones
R437	T2411	T2409	cc	and,chaperones
R438	T2412	T2413	compound	chromatin,remodeling
R439	T2413	T2414	compound	remodeling,complexes
R440	T2414	T2409	conj	complexes,chaperones
R441	T2415	T2406	aux	can,collaborate
R442	T2416	T2417	aux	to,promote
R443	T2417	T2406	advcl	promote,collaborate
R444	T2418	T2419	compound	nucleosome,assembly
R445	T2419	T2417	dobj	assembly,promote
R446	T2420	T2421	advmod	in,vitro
R447	T2421	T2417	advmod	vitro,promote
R448	T2422	T2406	cc	and,collaborate
R449	T2423	T2406	conj	are,collaborate
R450	T2424	T2423	advmod	therefore,are
R451	T2425	T2423	prep	in,are
R452	T2426	T2427	det	a,position
R453	T2427	T2425	pobj	position,in
R454	T2428	T2427	amod	strategic,position
R455	T2429	T2430	aux	to,control
R456	T2430	T2427	advcl	control,position
R457	T2431	T2432	compound	chromatin,assembly
R458	T2432	T2430	dobj	assembly,control
R459	T2433	T2432	cc	and,assembly
R460	T2434	T2432	conj	maturation,assembly
R461	T2435	T2430	prep	during,control
R462	T2436	T2435	pobj	development,during
R463	T2437	T2438	punct	[,8
R464	T2438	T2423	parataxis	8,are
R465	T2439	T2438	nummod	7,8
R466	T2440	T2438	punct	",",8
R467	T2441	T2438	punct	],8
R468	T2442	T2406	punct	.,collaborate
R469	T2444	T2445	prep	Among,is
R470	T2446	T2447	compound	histone,chaperones
R471	T2447	T2444	pobj	chaperones,Among
R472	T2448	T2445	punct	", ",is
R473	T2449	T2450	compound	chromatin,assembly
R474	T2450	T2451	compound	assembly,factor
R475	T2451	T2445	nsubj	factor,is
R476	T2452	T2451	nummod	1,factor
R477	T2453	T2451	punct	(,factor
R478	T2454	T2451	appos	CAF,factor
R479	T2455	T2454	punct	-,CAF
R480	T2456	T2454	nummod	1,CAF
R481	T2457	T2445	punct	),is
R482	T2458	T2459	det	a,complex
R483	T2459	T2445	attr	complex,is
R484	T2460	T2461	nummod	three,subunit
R485	T2461	T2459	nmod	subunit,complex
R486	T2462	T2461	punct	-,subunit
R487	T2463	T2464	punct	(,p150
R488	T2464	T2461	parataxis	p150,subunit
R489	T2465	T2464	punct	", ",p150
R490	T2466	T2464	conj	p60,p150
R491	T2467	T2466	punct	", ",p60
R492	T2468	T2466	cc	and,p60
R493	T2469	T2466	conj	p48,p60
R494	T2470	T2464	punct	),p150
R495	T2471	T2472	dep	which,promotes
R496	T2472	T2459	relcl	promotes,complex
R497	T2473	T2474	nmod	histone,H3
R498	T2474	T2475	nmod	H3,deposition
R499	T2475	T2472	dobj	deposition,promotes
R500	T2476	T2474	cc	and,H3
R501	T2477	T2474	conj	H4,H3
R502	T2478	T2475	prep	onto,deposition
R503	T2479	T2480	advmod	newly,synthesized
R504	T2480	T2481	amod	synthesized,DNA
R505	T2481	T2478	pobj	DNA,onto
R506	T2482	T2472	prep	during,promotes
R507	T2483	T2482	pobj	replication,during
R508	T2484	T2483	cc	or,replication
R509	T2485	T2486	compound	DNA,repair
R510	T2486	T2483	conj	repair,replication
R511	T2487	T2488	punct	[,10
R512	T2488	T2445	parataxis	10,is
R513	T2489	T2488	nummod	9,10
R514	T2490	T2488	punct	",",10
R515	T2491	T2488	punct	],10
R516	T2492	T2445	punct	.,is
R517	T2494	T2495	advmod	Specifically,deposits
R518	T2496	T2495	punct	", ",deposits
R519	T2497	T2495	nsubj	CAF,deposits
R520	T2498	T2497	punct	-,CAF
R521	T2499	T2497	nummod	1,CAF
R522	T2500	T2501	det	the,variant
R523	T2501	T2495	dobj	variant,deposits
R524	T2502	T2503	compound	histone,H3
R525	T2503	T2501	compound	H3,variant
R526	T2504	T2501	appos	H3.1,variant
R527	T2505	T2495	prep	into,deposits
R528	T2506	T2505	pobj	chromatin,into
R529	T2507	T2495	punct	", ",deposits
R530	T2508	T2495	prep	in,deposits
R531	T2509	T2510	det	a,pathway
R532	T2510	T2508	pobj	pathway,in
R533	T2511	T2510	acl	coupled,pathway
R534	T2512	T2511	prep	to,coupled
R535	T2513	T2514	compound	DNA,synthesis
R536	T2514	T2512	pobj	synthesis,to
R537	T2515	T2495	punct	", ",deposits
R538	T2516	T2517	mark	whereas,involved
R539	T2517	T2495	advcl	involved,deposits
R540	T2518	T2519	det	a,chaperone
R541	T2519	T2517	nsubjpass	chaperone,involved
R542	T2520	T2519	amod	second,chaperone
R543	T2521	T2519	compound	histone,chaperone
R544	T2522	T2519	punct	", ",chaperone
R545	T2523	T2519	appos	HIRA,chaperone
R546	T2524	T2517	punct	", ",involved
R547	T2525	T2517	auxpass	is,involved
R548	T2526	T2517	prep	in,involved
R549	T2527	T2528	det	the,deposition
R550	T2528	T2526	pobj	deposition,in
R551	T2529	T2528	prep	of,deposition
R552	T2530	T2531	det	the,variant
R553	T2531	T2529	pobj	variant,of
R554	T2532	T2531	compound	H3.3,variant
R555	T2533	T2528	prep	in,deposition
R556	T2534	T2535	det	a,pathway
R557	T2535	T2533	pobj	pathway,in
R558	T2536	T2537	nmod	DNA,synthesis
R559	T2537	T2535	nmod	synthesis,pathway
R560	T2538	T2535	amod	independent,pathway
R561	T2539	T2540	punct	[,12
R562	T2540	T2495	parataxis	12,deposits
R563	T2541	T2540	nummod	11,12
R564	T2542	T2540	punct	",",12
R565	T2543	T2540	punct	],12
R566	T2544	T2495	punct	.,deposits
R567	T2546	T2547	prep	In,interacts
R568	T2548	T2546	pobj	addition,In
R569	T2549	T2548	prep	to,addition
R570	T2550	T2551	det	this,activity
R571	T2551	T2549	pobj	activity,to
R572	T2552	T2551	amod	specific,activity
R573	T2553	T2554	compound	chromatin,assembly
R574	T2554	T2551	compound	assembly,activity
R575	T2555	T2547	punct	", ",interacts
R576	T2556	T2547	nsubj	CAF,interacts
R577	T2557	T2556	punct	-,CAF
R578	T2558	T2556	nummod	1,CAF
R579	T2559	T2547	prep	with,interacts
R580	T2560	T2561	amod	several,proteins
R581	T2561	T2559	pobj	proteins,with
R582	T2562	T2561	relcl	present,proteins
R583	T2563	T2562	prep	in,present
R584	T2564	T2563	pobj	heterochromatin,in
R585	T2565	T2561	punct	", ",proteins
R586	T2566	T2561	prep	including,proteins
R587	T2567	T2568	compound	heterochromatin,protein
R588	T2568	T2566	pobj	protein,including
R589	T2569	T2568	nummod	1,protein
R590	T2570	T2568	punct	(,protein
R591	T2571	T2568	appos	HP1,protein
R592	T2572	T2568	punct	),protein
R593	T2573	T2574	cc	and,MBD1
R594	T2574	T2568	appos	MBD1,protein
R595	T2575	T2574	punct	", ",MBD1
R596	T2576	T2577	det	a,protein
R597	T2577	T2574	appos	protein,MBD1
R598	T2578	T2579	compound	methyl,CpG
R599	T2579	T2577	compound	CpG,protein
R600	T2580	T2579	punct	-,CpG
R601	T2581	T2582	compound	binding,domain
R602	T2582	T2577	compound	domain,protein
R603	T2583	T2584	dep	that,recruits
R604	T2584	T2577	relcl	recruits,protein
R605	T2585	T2586	nmod	histone,deacetylase
R606	T2586	T2587	nmod	deacetylase,activities
R607	T2587	T2584	dobj	activities,recruits
R608	T2588	T2586	cc	and,deacetylase
R609	T2589	T2590	amod	repressive,methyltransferase
R610	T2590	T2586	conj	methyltransferase,deacetylase
R611	T2591	T2590	compound	histone,methyltransferase
R612	T2592	T2593	punct	[,13
R613	T2593	T2547	parataxis	13,interacts
R614	T2594	T2595	punct	–,15
R615	T2595	T2593	prep	15,13
R616	T2596	T2593	punct	],13
R617	T2597	T2547	punct	.,interacts
R618	T2599	T2600	advmod	Furthermore,required
R619	T2601	T2600	punct	", ",required
R620	T2602	T2600	nsubjpass	p150CAF,required
R621	T2603	T2602	punct	-,p150CAF
R622	T2604	T2602	nummod	1,p150CAF
R623	T2605	T2600	auxpass	is,required
R624	T2606	T2607	aux	to,ensure
R625	T2607	T2600	advcl	ensure,required
R626	T2608	T2609	det	a,pool
R627	T2609	T2607	dobj	pool,ensure
R628	T2610	T2611	npadvmod	replication,specific
R629	T2611	T2609	amod	specific,pool
R630	T2612	T2611	punct	-,specific
R631	T2613	T2609	prep	of,pool
R632	T2614	T2615	compound	HP1,molecules
R633	T2615	T2613	pobj	molecules,of
R634	T2616	T2607	prep	at,ensure
R635	T2617	T2618	compound	replication,sites
R636	T2618	T2616	pobj	sites,at
R637	T2619	T2618	prep	in,sites
R638	T2620	T2621	amod	pericentric,heterochromatin
R639	T2621	T2619	pobj	heterochromatin,in
R640	T2622	T2607	prep	during,ensure
R641	T2623	T2624	amod	mid,late
R642	T2624	T2626	amod	late,phase
R643	T2625	T2624	punct	-,late
R644	T2626	T2622	pobj	phase,during
R645	T2627	T2626	compound	S,phase
R646	T2628	T2629	punct	[,16
R647	T2629	T2600	parataxis	16,required
R648	T2630	T2629	punct	],16
R649	T2631	T2600	punct	.,required
R650	T2633	T2634	advcl	Taken,suggest
R651	T2635	T2633	advmod	together,Taken
R652	T2636	T2634	punct	", ",suggest
R653	T2637	T2638	det	these,data
R654	T2638	T2634	nsubj	data,suggest
R655	T2639	T2634	dobj	roles,suggest
R656	T2640	T2639	prep	for,roles
R657	T2641	T2640	pobj	CAF,for
R658	T2642	T2641	punct	-,CAF
R659	T2643	T2641	nummod	1,CAF
R660	T2644	T2639	prep	in,roles
R661	T2645	T2646	det	the,formation
R662	T2646	T2644	pobj	formation,in
R663	T2647	T2646	prep	of,formation
R664	T2648	T2647	pobj	heterochromatin,of
R665	T2649	T2644	cc	and,in
R666	T2650	T2644	conj	in,in
R667	T2651	T2652	det	the,heritability
R668	T2652	T2650	pobj	heritability,in
R669	T2653	T2652	prep	of,heritability
R670	T2654	T2655	amod	epigenetic,traits
R671	T2655	T2653	pobj	traits,of
R672	T2656	T2634	punct	.,suggest
R673	T2658	T2659	mark	While,studied
R674	T2659	T2670	advcl	studied,lack
R675	T2660	T2661	det	the,function
R676	T2661	T2659	nsubjpass	function,studied
R677	T2662	T2661	prep	of,function
R678	T2663	T2664	det	this,chaperone
R679	T2664	T2662	pobj	chaperone,of
R680	T2665	T2666	amod	evolutionary,conserved
R681	T2666	T2664	amod	conserved,chaperone
R682	T2667	T2664	compound	histone,chaperone
R683	T2668	T2659	aux	has,studied
R684	T2669	T2659	auxpass	been,studied
R685	T2671	T2659	advmod	extensively,studied
R686	T2672	T2659	advmod	biochemically,studied
R687	T2673	T2670	punct	", ",lack
R688	T2674	T2670	nsubj	we,lack
R689	T2675	T2670	advmod	still,lack
R690	T2676	T2670	dobj	information,lack
R691	T2677	T2676	prep	concerning,information
R692	T2678	T2679	poss	its,importance
R693	T2679	T2677	pobj	importance,concerning
R694	T2680	T2679	prep	during,importance
R695	T2681	T2682	amod	early,development
R696	T2682	T2680	pobj	development,during
R697	T2683	T2679	prep	in,importance
R698	T2684	T2683	pobj	mammals,in
R699	T2685	T2683	cc	and,in
R700	T2686	T2683	conj	in,in
R701	T2687	T2688	amod	pluripotent,cells
R702	T2688	T2686	pobj	cells,in
R703	T2689	T2690	amod	such,as
R704	T2690	T2688	prep	as,cells
R705	T2691	T2692	amod	embryonic,stem
R706	T2692	T2693	nmod	stem,cells
R707	T2693	T2690	pobj	cells,as
R708	T2694	T2692	punct	(,stem
R709	T2695	T2692	appos	ES,stem
R710	T2696	T2693	punct	),cells
R711	T2697	T2670	punct	.,lack
R712	T2699	T2700	advmod	Here,analyzed
R713	T2701	T2700	punct	", ",analyzed
R714	T2702	T2700	nsubj	we,analyzed
R715	T2703	T2700	aux	have,analyzed
R716	T2704	T2705	det	the,importance
R717	T2705	T2700	dobj	importance,analyzed
R718	T2706	T2705	prep	of,importance
R719	T2707	T2706	pobj	CAF,of
R720	T2708	T2707	punct	-,CAF
R721	T2709	T2707	nummod	1,CAF
R722	T2710	T2705	prep	during,importance
R723	T2711	T2712	amod	early,development
R724	T2712	T2710	pobj	development,during
R725	T2713	T2712	compound	mouse,development
R726	T2714	T2700	prep	by,analyzed
R727	T2715	T2716	amod	genetic,ablation
R728	T2716	T2714	pobj	ablation,by
R729	T2717	T2700	cc	and,analyzed
R730	T2718	T2700	conj	in,analyzed
R731	T2719	T2720	compound	ES,cells
R732	T2720	T2718	pobj	cells,in
R733	T2721	T2718	prep	by,in
R734	T2722	T2721	pobj	depletion,by
R735	T2723	T2722	acl	using,depletion
R736	T2724	T2725	compound	RNA,interference
R737	T2725	T2723	dobj	interference,using
R738	T2726	T2725	punct	(,interference
R739	T2727	T2725	appos	RNAi,interference
R740	T2728	T2700	punct	),analyzed
R741	T2729	T2700	punct	.,analyzed
R742	T2731	T2732	nsubj	We,show
R743	T2733	T2734	mark	that,is
R744	T2734	T2732	ccomp	is,show
R745	T2735	T2734	nsubj	CAF,is
R746	T2736	T2735	punct	-,CAF
R747	T2737	T2735	nummod	1,CAF
R748	T2738	T2734	acomp	essential,is
R749	T2739	T2738	prep	for,essential
R750	T2740	T2739	pobj	viability,for
R751	T2741	T2734	prep	in,is
R752	T2742	T2743	amod	early,embryos
R753	T2743	T2741	pobj	embryos,in
R754	T2744	T2743	compound	mouse,embryos
R755	T2745	T2743	cc	and,embryos
R756	T2746	T2747	compound	ES,cells
R757	T2747	T2743	conj	cells,embryos
R758	T2748	T2732	punct	.,show
R759	T2750	T2751	nsubj	We,provide
R760	T2752	T2751	dobj	evidence,provide
R761	T2753	T2754	mark	that,required
R762	T2754	T2752	acl	required,evidence
R763	T2755	T2754	nsubjpass	CAF,required
R764	T2756	T2755	punct	-,CAF
R765	T2757	T2755	nummod	1,CAF
R766	T2758	T2754	auxpass	is,required
R767	T2759	T2754	prep	for,required
R768	T2760	T2761	det	the,organization
R769	T2761	T2759	pobj	organization,for
R770	T2762	T2761	amod	spatial,organization
R771	T2763	T2761	cc	and,organization
R772	T2764	T2765	amod	epigenetic,marking
R773	T2765	T2761	conj	marking,organization
R774	T2766	T2761	prep	of,organization
R775	T2767	T2768	compound	heterochromatin,domains
R776	T2768	T2766	pobj	domains,of
R777	T2769	T2768	prep	in,domains
R778	T2770	T2771	amod	pluripotent,cells
R779	T2771	T2769	pobj	cells,in
R780	T2772	T2771	amod	embryonic,cells
R781	T2773	T2751	punct	.,provide
R795	T7971	T7972	nsubj	We,used
R805	T7973	T7974	compound	gene,targeting
R806	T7974	T7972	dobj	targeting,used
R807	T7975	T7972	prep	in,used
R808	T7976	T7977	compound	ES,cells
R809	T7977	T7975	pobj	cells,in
R810	T7978	T7979	aux	to,delete
R811	T8011	T8010	prep	of,were
R812	T7979	T7972	advcl	delete,used
R813	T8012	T8013	amod	normal,size
R814	T8013	T8011	pobj	size,of
R815	T8014	T8013	cc	and,size
R816	T8015	T8013	conj	weight,size
R817	T7980	T7979	dobj	exon,delete
R818	T8016	T8010	punct	", ",were
R819	T8017	T8010	conj	displayed,were
R820	T8018	T8019	det	no,abnormalities
R821	T8019	T8017	dobj	abnormalities,displayed
R822	T7981	T7980	nummod	3,exon
R823	T8020	T8019	amod	obvious,abnormalities
R824	T7982	T7979	prep	in,delete
R825	T8021	T8017	punct	", ",displayed
R826	T8022	T8017	cc	and,displayed
R827	T8023	T8017	conj	were,displayed
R828	T8024	T8023	acomp	fertile,were
R829	T8025	T8003	punct	.,born
R830	T7983	T7984	det	the,gene
R831	T8027	T8028	csubj	Crossing,failed
R832	T8029	T8030	amod	heterozygous,mice
R833	T8030	T8027	dobj	mice,Crossing
R834	T8031	T8032	aux	to,generate
R835	T8032	T8028	xcomp	generate,failed
R836	T8033	T8034	amod	viable,mice
R837	T8034	T8032	dobj	mice,generate
R838	T8035	T8034	amod	newborn,mice
R839	T8036	T8034	nmod	Chaf1a,mice
R840	T7984	T7982	pobj	gene,in
R841	T8037	T8036	punct	−,Chaf1a
R842	T8038	T8036	punct	/,Chaf1a
R843	T8039	T8036	punct	−,Chaf1a
R844	T8040	T8028	punct	.,failed
R845	T7985	T7984	compound	Chaf1a,gene
R846	T8042	T8043	advmod	Furthermore,detected
R847	T7986	T7984	punct	", ",gene
R848	T8044	T8043	punct	", ",detected
R849	T8045	T8046	det	no,embryos
R850	T8046	T8043	nsubjpass	embryos,detected
R851	T8047	T8046	amod	homozygous,embryos
R852	T7987	T7988	dep	which,encodes
R853	T8048	T8046	nmod	Chaf1a,embryos
R854	T8049	T8048	punct	−,Chaf1a
R855	T8050	T8048	punct	/,Chaf1a
R856	T7988	T7984	relcl	encodes,gene
R857	T8051	T8048	punct	−,Chaf1a
R858	T8052	T8043	auxpass	were,detected
R859	T8053	T8043	prep	at,detected
R860	T7989	T7988	dobj	p150CAF,encodes
R861	T8054	T8053	pobj	any,at
R862	T8055	T8054	prep	of,any
R863	T8056	T8057	det	the,stages
R864	T7990	T7989	punct	-,p150CAF
R865	T8057	T8055	pobj	stages,of
R866	T8058	T8057	amod	post-implantation,stages
R867	T8059	T8043	punct	.,detected
R868	T7991	T7989	nummod	1,p150CAF
R869	T8061	T8062	advmod	However,detect
R870	T7992	T7993	punct	(,1A
R871	T8063	T8062	punct	", ",detect
R872	T8064	T8062	nsubj	we,detect
R873	T8065	T8062	aux	could,detect
R874	T7993	T7972	parataxis	1A,used
R875	T8066	T8067	nmod	Chaf1a,embryos
R876	T8067	T8062	dobj	embryos,detect
R877	T8068	T8066	punct	−,Chaf1a
R878	T7994	T7993	compound	Figure,1A
R879	T8069	T8066	punct	/,Chaf1a
R880	T8070	T8066	punct	−,Chaf1a
R881	T8071	T8062	prep	at,detect
R882	T8072	T8073	amod	embryonic,day
R883	T8073	T8071	pobj	day,at
R884	T8074	T8073	nummod	4,day
R885	T8075	T8073	punct	(,day
R886	T7995	T7993	punct	),1A
R887	T8076	T8073	appos	E4,day
R888	T7996	T7972	punct	.,used
R889	T8077	T8062	punct	),detect
R890	T8078	T8062	advcl	using,detect
R891	T7998	T7999	nmod	Chaf1a,mice
R892	T8079	T8080	det	a,strategy
R893	T8080	T8078	dobj	strategy,using
R894	T8081	T8080	compound	PCR,strategy
R895	T8082	T8083	punct	(,1B
R896	T7999	T8003	nsubjpass	mice,born
R897	T8083	T8062	parataxis	1B,detect
R898	T8084	T8083	compound	Figure,1B
R899	T8085	T8083	punct	),1B
R900	T8000	T7998	punct	+,Chaf1a
R901	T8086	T8062	punct	.,detect
R902	T8088	T8089	nsubj	They,represent
R903	T8001	T7998	punct	/,Chaf1a
R904	T8090	T8091	advmod	only,10
R905	T8091	T8092	nummod	10,%
R906	T8002	T7998	punct	−,Chaf1a
R907	T8092	T8089	dobj	%,represent
R908	T8093	T8092	prep	of,%
R909	T8094	T8095	compound	E4,embryos
R910	T8004	T8003	auxpass	were,born
R911	T8095	T8093	pobj	embryos,of
R912	T8096	T8092	acl	obtained,%
R913	T8097	T8096	prep	from,obtained
R914	T8098	T8099	nmod	Chaf1a,intercrosses
R915	T8005	T8003	prep	at,born
R916	T8099	T8097	pobj	intercrosses,from
R917	T8100	T8098	punct	+,Chaf1a
R918	T8101	T8098	punct	/,Chaf1a
R919	T8006	T8007	det	a,frequency
R920	T8102	T8098	punct	−,Chaf1a
R921	T8103	T8099	compound	mice,intercrosses
R922	T8104	T8089	punct	", ",represent
R923	T8105	T8089	advcl	suggesting,represent
R924	T8007	T8005	pobj	frequency,at
R925	T8106	T8107	mark	that,degenerated
R926	T8107	T8105	ccomp	degenerated,suggesting
R927	T8008	T8007	amod	Mendelian,frequency
R928	T8108	T8109	amod	more,half
R929	T8109	T8107	nsubj	half,degenerated
R930	T8110	T8109	quantmod	than,half
R931	T8111	T8109	prep	of,half
R932	T8009	T8003	punct	", ",born
R933	T8112	T8113	det	the,embryos
R934	T8113	T8111	pobj	embryos,of
R935	T8114	T8115	amod	homozygous,mutant
R936	T8115	T8113	compound	mutant,embryos
R937	T8116	T8107	aux	had,degenerated
R938	T8010	T8003	conj	were,born
R939	T8223	T8224	advmod	Thus,allows
R940	T8117	T8107	prep	before,degenerated
R941	T8118	T8119	det	this,stage
R942	T8119	T8117	pobj	stage,before
R943	T8120	T8089	punct	.,represent
R944	T8225	T8224	punct	", ",allows
R945	T8122	T8123	advmod	Moreover,contained
R946	T8124	T8123	punct	", ",contained
R947	T8125	T8126	compound	mutant,embryos
R948	T8126	T8123	nsubj	embryos,contained
R949	T8226	T8224	nsubj	depletion,allows
R950	T8127	T8128	advmod	only,16
R951	T8128	T8131	nummod	16,cells
R952	T8129	T8128	quantmod	eight,16
R953	T8227	T8226	prep	of,depletion
R954	T8130	T8128	quantmod	to,16
R955	T8131	T8123	dobj	cells,contained
R956	T8132	T8123	prep	at,contained
R957	T8228	T8229	nmod	p150CAF,protein
R958	T8133	T8134	det	the,stage
R959	T8134	T8132	pobj	stage,at
R960	T8135	T8134	compound	E4,stage
R961	T8229	T8227	pobj	protein,of
R962	T8136	T8123	punct	", ",contained
R963	T8137	T8138	advmod	instead,of
R964	T8138	T8123	prep	of,contained
R965	T8230	T8228	punct	-,p150CAF
R966	T8139	T8140	nummod	32,cells
R967	T8140	T8138	pobj	cells,of
R968	T8141	T8140	acl	observed,cells
R969	T8231	T8228	nummod	1,p150CAF
R970	T8142	T8141	prep	for,observed
R971	T8143	T8144	amod	wild,type
R972	T8144	T8146	nmod	type,blastocysts
R973	T8232	T8226	prep	from,depletion
R974	T8145	T8144	punct	-,type
R975	T8146	T8142	pobj	blastocysts,for
R976	T8147	T8144	cc	or,type
R977	T8233	T8234	det	this,stage
R978	T8148	T8144	conj	heterozygous,type
R979	T8234	T8232	pobj	stage,from
R980	T8149	T8150	punct	(,1C
R981	T8150	T8123	parataxis	1C,contained
R982	T8151	T8150	compound	Figure,1C
R983	T8152	T8150	cc	and,1C
R984	T8235	T8236	det	a,maximum
R985	T8153	T8150	conj	1D,1C
R986	T8154	T8150	punct	),1C
R987	T8155	T8123	punct	.,contained
R988	T8157	T8158	amod	Further,analysis
R989	T8158	T8159	nsubj	analysis,allowed
R990	T8236	T8224	dobj	maximum,allows
R991	T8160	T8158	prep	by,analysis
R992	T8161	T8162	compound	light,microscopy
R993	T8237	T8236	prep	of,maximum
R994	T8162	T8160	pobj	microscopy,by
R995	T8238	T8239	nummod	two,divisions
R996	T8163	T8162	acl	using,microscopy
R997	T8164	T8163	dobj	immunofluorescence,using
R998	T8239	T8237	pobj	divisions,of
R999	T8165	T8166	punct	(,IF
R1000	T8166	T8164	parataxis	IF,immunofluorescence
R1001	T8167	T8166	punct	),IF
R1002	T8240	T8239	amod	additional,divisions
R1003	T8168	T8169	nsubj	us,compare
R1004	T8169	T8159	ccomp	compare,allowed
R1005	T8170	T8169	aux	to,compare
R1006	T8241	T8239	compound	cell,divisions
R1007	T8171	T8172	amod	wild,type
R1008	T8172	T8174	nmod	type,embryos
R1009	T8173	T8172	punct	-,type
R1010	T8242	T8224	prep	before,allows
R1011	T8174	T8169	dobj	embryos,compare
R1012	T8175	T8172	cc	and,type
R1013	T8176	T8177	npadvmod	p150CAF,depleted
R1014	T8177	T8172	conj	depleted,type
R1015	T8243	T8244	amod	developmental,arrest
R1016	T8244	T8242	pobj	arrest,before
R1017	T8178	T8176	punct	-,p150CAF
R1018	T8245	T8224	punct	.,allows
R1019	T8179	T8176	nummod	1,p150CAF
R1020	T8180	T8177	punct	-,depleted
R1021	T8247	T8248	advmod	Strikingly,found
R1022	T8181	T8159	punct	.,allowed
R1023	T8183	T8184	det	A,loss
R1024	T8184	T8185	nsubjpass	loss,detected
R1025	T8249	T8248	punct	", ",found
R1026	T8186	T8184	prep	of,loss
R1027	T8187	T8188	nmod	p150CAF,staining
R1028	T8188	T8186	pobj	staining,of
R1029	T8189	T8187	punct	-,p150CAF
R1030	T8250	T8248	nsubj	we,found
R1031	T8190	T8187	nummod	1,p150CAF
R1032	T8191	T8185	aux	could,detected
R1033	T8251	T8252	mark	that,appeared
R1034	T8192	T8185	neg	not,detected
R1035	T8193	T8185	auxpass	be,detected
R1036	T8194	T8185	prep	before,detected
R1037	T8195	T8196	det	the,stage
R1038	T8252	T8248	ccomp	appeared,found
R1039	T8196	T8194	pobj	stage,before
R1040	T8197	T8198	nummod	eight,cell
R1041	T8198	T8196	compound	cell,stage
R1042	T8199	T8198	punct	-,cell
R1043	T8200	T8201	punct	(,data
R1044	T8201	T8185	meta	data,detected
R1045	T8253	T8254	det	the,organization
R1046	T8202	T8201	amod	unpublished,data
R1047	T8203	T8201	punct	),data
R1048	T8204	T8185	advcl	suggesting,detected
R1049	T8254	T8252	nsubj	organization,appeared
R1050	T8205	T8206	mark	that,is
R1051	T8206	T8204	ccomp	is,suggesting
R1052	T8207	T8208	advmod	maternally,contributed
R1053	T8255	T8254	amod	nuclear,organization
R1054	T8208	T8209	amod	contributed,protein
R1055	T8209	T8206	nsubj	protein,is
R1056	T8210	T8206	acomp	present,is
R1057	T8256	T8254	prep	of,organization
R1058	T8211	T8206	prep	in,is
R1059	T8212	T8213	det	the,embryo
R1060	T8213	T8211	pobj	embryo,in
R1061	T8257	T8256	pobj	heterochromatin,of
R1062	T8214	T8206	prep	up,is
R1063	T8215	T8214	prep	to,up
R1064	T8216	T8217	det	the,stage
R1065	T8258	T8252	oprd	abnormal,appeared
R1066	T8217	T8215	pobj	stage,to
R1067	T8218	T8219	nummod	four,cell
R1068	T8219	T8217	compound	cell,stage
R1069	T8259	T8252	prep	in,appeared
R1070	T8220	T8219	punct	-,cell
R1071	T8221	T8185	punct	.,detected
R1072	T8260	T8261	det	the,nuclei
R1073	T8261	T8259	pobj	nuclei,in
R1074	T8262	T8261	prep	of,nuclei
R1075	T8328	T8326	acl	called,structures
R1076	T8329	T8328	oprd	chromocenters,called
R1077	T8263	T8264	nmod	Chaf1a,embryos
R1078	T8330	T8331	punct	[,20
R1079	T8331	T8328	parataxis	20,called
R1080	T8332	T8331	punct	],20
R1081	T8264	T8262	pobj	embryos,of
R1082	T8333	T8326	punct	", ",structures
R1083	T8334	T8335	dep	which,visualized
R1084	T8265	T8263	punct	−,Chaf1a
R1085	T8335	T8326	relcl	visualized,structures
R1086	T8266	T8263	punct	/,Chaf1a
R1087	T8336	T8335	auxpass	are,visualized
R1088	T8267	T8263	punct	−,Chaf1a
R1089	T8337	T8335	prep	as,visualized
R1090	T8338	T8339	npadvmod	DAPI,dense
R1091	T8339	T8341	amod	dense,foci
R1092	T8340	T8339	punct	-,dense
R1093	T8341	T8337	pobj	foci,as
R1094	T8342	T8343	punct	(,1C
R1095	T8268	T8248	punct	.,found
R1096	T8343	T8322	parataxis	1C,form
R1097	T8344	T8343	compound	Figure,1C
R1098	T8345	T8343	punct	),1C
R1099	T8270	T8271	amod	Pericentric,heterochromatin
R1100	T8346	T8306	punct	.,cluster
R1101	T8348	T8349	det	These,structures
R1102	T8349	T8350	nsubjpass	structures,detected
R1103	T8271	T8272	nsubjpass	heterochromatin,visualized
R1104	T8351	T8350	auxpass	were,detected
R1105	T8352	T8350	neg	not,detected
R1106	T8273	T8271	punct	", ",heterochromatin
R1107	T8353	T8350	prep	in,detected
R1108	T8354	T8355	nmod	Chaf1a,embryos
R1109	T8355	T8353	pobj	embryos,in
R1110	T8356	T8354	punct	−,Chaf1a
R1111	T8274	T8275	det	the,component
R1112	T8357	T8354	punct	/,Chaf1a
R1113	T8358	T8354	punct	−,Chaf1a
R1114	T8359	T8360	punct	(,1D
R1115	T8275	T8271	appos	component,heterochromatin
R1116	T8360	T8350	parataxis	1D,detected
R1117	T8361	T8360	compound	Figure,1D
R1118	T8362	T8360	punct	),1D
R1119	T8276	T8275	amod	major,component
R1120	T8363	T8350	punct	.,detected
R1121	T8365	T8366	mark	Since,are
R1122	T8277	T8275	prep	of,component
R1123	T8366	T8374	advcl	are,decided
R1124	T8367	T8368	compound	chromatin,architecture
R1125	T8368	T8366	nsubj	architecture,are
R1126	T8278	T8279	amod	constitutive,heterochromatin
R1127	T8369	T8368	cc	and,architecture
R1128	T8370	T8371	amod	higher,order
R1129	T8371	T8373	compound	order,structures
R1130	T8372	T8371	punct	-,order
R1131	T8279	T8277	pobj	heterochromatin,of
R1132	T8373	T8368	conj	structures,architecture
R1133	T8280	T8272	punct	", ",visualized
R1134	T8375	T8366	acomp	important,are
R1135	T8376	T8375	prep	for,important
R1136	T8377	T8378	compound	genome,function
R1137	T8281	T8272	aux	can,visualized
R1138	T8378	T8376	pobj	function,for
R1139	T8379	T8380	punct	[,21
R1140	T8282	T8272	advmod	easily,visualized
R1141	T8380	T8366	parataxis	21,are
R1142	T8381	T8380	punct	],21
R1143	T8382	T8374	punct	", ",decided
R1144	T8383	T8374	nsubj	we,decided
R1145	T8384	T8385	aux	to,characterize
R1146	T8385	T8374	xcomp	characterize,decided
R1147	T8283	T8272	auxpass	be,visualized
R1148	T8386	T8387	det	this,phenotype
R1149	T8387	T8385	dobj	phenotype,characterize
R1150	T8388	T8385	prep	in,characterize
R1151	T8284	T8272	prep	in,visualized
R1152	T8389	T8390	amod	more,detail
R1153	T8390	T8388	pobj	detail,in
R1154	T8391	T8374	punct	.,decided
R1155	T8285	T8286	compound	interphase,nuclei
R1156	T8393	T8394	prep	For,examined
R1157	T8286	T8284	pobj	nuclei,in
R1158	T8395	T8396	det	this,purpose
R1159	T8396	T8393	pobj	purpose,For
R1160	T8397	T8394	punct	", ",examined
R1161	T8287	T8272	prep	by,visualized
R1162	T8398	T8394	nsubj	we,examined
R1163	T8399	T8400	det	the,status
R1164	T8400	T8394	dobj	status,examined
R1165	T8288	T8289	det	the,DAPI
R1166	T8401	T8400	prep	of,status
R1167	T8402	T8403	compound	heterochromatin,organization
R1168	T8403	T8401	pobj	organization,of
R1169	T8289	T8287	pobj	DAPI,by
R1170	T8404	T8394	prep	between,examined
R1171	T8405	T8406	det	the,stages
R1172	T8406	T8404	pobj	stages,between
R1173	T8407	T8408	nummod	two,cell
R1174	T8290	T8289	compound	fluorochrome,DAPI
R1175	T8408	T8406	nmod	cell,stages
R1176	T8409	T8408	punct	-,cell
R1177	T8410	T8408	cc	and,cell
R1178	T8291	T8287	cc	and,by
R1179	T8411	T8408	conj	blastocyst,cell
R1180	T8412	T8394	punct	.,examined
R1181	T8292	T8287	conj	by,by
R1182	T8414	T8415	prep	In,is
R1183	T8293	T8292	pcomp	immunostaining,by
R1184	T8416	T8417	nummod	two,cell
R1185	T8417	T8419	nmod	cell,stage
R1186	T8294	T8293	prep	with,immunostaining
R1187	T8418	T8417	punct	-,cell
R1188	T8419	T8420	nmod	stage,embryos
R1189	T8420	T8414	pobj	embryos,In
R1190	T8295	T8294	pobj	HP1α,with
R1191	T8421	T8422	amod	wild,type
R1192	T8422	T8420	compound	type,embryos
R1193	T8423	T8422	punct	-,type
R1194	T8296	T8297	punct	[,16
R1195	T8424	T8415	punct	", ",is
R1196	T8425	T8426	compound	DAPI,staining
R1197	T8426	T8415	nsubj	staining,is
R1198	T8297	T8272	parataxis	16,visualized
R1199	T8427	T8415	acomp	diffuse,is
R1200	T8428	T8427	cc	and,diffuse
R1201	T8429	T8427	conj	fibrillar,diffuse
R1202	T8430	T8415	punct	", ",is
R1203	T8298	T8297	punct	",",16
R1204	T8431	T8415	prep	with,is
R1205	T8432	T8431	pobj	regions,with
R1206	T8299	T8297	appos	17,16
R1207	T8300	T8301	punct	–,19
R1208	T8301	T8299	prep	19,17
R1209	T8302	T8297	punct	],16
R1210	T8433	T8432	prep	of,regions
R1211	T8303	T8272	punct	.,visualized
R1212	T8434	T8435	amod	higher,density
R1213	T8435	T8433	pobj	density,of
R1214	T8436	T8432	prep	around,regions
R1215	T8305	T8306	prep	In,cluster
R1216	T8437	T8438	det	the,bodies
R1217	T8438	T8436	pobj	bodies,around
R1218	T8439	T8440	amod	nucleolar,precursor
R1219	T8307	T8308	amod	wild,type
R1220	T8440	T8438	compound	precursor,bodies
R1221	T8441	T8442	punct	(,1E
R1222	T8442	T8415	parataxis	1E,is
R1223	T8443	T8442	compound	Figure,1E
R1224	T8444	T8442	punct	),1E
R1225	T8308	T8310	compound	type,blastocysts
R1226	T8445	T8415	punct	.,is
R1227	T8447	T8448	compound	Heterochromatin,domains
R1228	T8309	T8308	punct	-,type
R1229	T8448	T8449	nsubjpass	domains,assembled
R1230	T8310	T8305	pobj	blastocysts,In
R1231	T8450	T8449	auxpass	are,assembled
R1232	T8451	T8449	advmod	progressively,assembled
R1233	T8452	T8449	prep	into,assembled
R1234	T8311	T8305	cc	and,In
R1235	T8453	T8454	npadvmod	DAPI,dense
R1236	T8454	T8456	amod	dense,foci
R1237	T8455	T8454	punct	-,dense
R1238	T8456	T8452	pobj	foci,into
R1239	T8312	T8305	conj	in,In
R1240	T8457	T8449	prep	between,assembled
R1241	T8458	T8459	det	the,stages
R1242	T8313	T8314	nmod	mouse,cells
R1243	T8459	T8457	pobj	stages,between
R1244	T8460	T8461	nummod	four,cell
R1245	T8461	T8459	nmod	cell,stages
R1246	T8462	T8461	punct	-,cell
R1247	T8463	T8461	cc	and,cell
R1248	T8464	T8465	nummod	32,cell
R1249	T8314	T8312	pobj	cells,in
R1250	T8465	T8461	conj	cell,cell
R1251	T8466	T8465	punct	-,cell
R1252	T8467	T8459	compound	blastocyst,stages
R1253	T8468	T8469	punct	(,1F
R1254	T8315	T8314	amod	somatic,cells
R1255	T8469	T8449	parataxis	1F,assembled
R1256	T8470	T8469	compound	Figure,1F
R1257	T8471	T8469	punct	),1F
R1258	T8472	T8449	punct	.,assembled
R1259	T8316	T8306	punct	", ",cluster
R1260	T8474	T8475	nsubj	Localization,confirmed
R1261	T8317	T8318	amod	pericentric,domains
R1262	T8476	T8474	prep	of,Localization
R1263	T8477	T8476	pobj	HP1α,of
R1264	T8318	T8306	nsubj	domains,cluster
R1265	T8478	T8479	mark	that,correspond
R1266	T8479	T8475	ccomp	correspond,confirmed
R1267	T8480	T8481	det	the,structures
R1268	T8319	T8318	compound	heterochromatin,domains
R1269	T8481	T8479	nsubj	structures,correspond
R1270	T8482	T8481	acl	visualized,structures
R1271	T8483	T8482	prep	by,visualized
R1272	T8320	T8306	advmod	together,cluster
R1273	T8484	T8485	compound	DAPI,staining
R1274	T8485	T8483	pobj	staining,by
R1275	T8486	T8479	prep	to,correspond
R1276	T8321	T8306	cc	and,cluster
R1277	T8487	T8488	amod	constitutive,heterochromatin
R1278	T8488	T8486	pobj	heterochromatin,to
R1279	T8489	T8490	punct	(,1E
R1280	T8322	T8306	conj	form,cluster
R1281	T8490	T8475	parataxis	1E,confirmed
R1282	T8491	T8490	compound	Figure,1E
R1283	T8492	T8490	punct	),1E
R1284	T8323	T8324	amod	higher,order
R1285	T8493	T8475	punct	.,confirmed
R1286	T8495	T8496	advmod	Thus,modified
R1287	T8324	T8326	compound	order,structures
R1288	T8497	T8496	punct	", ",modified
R1289	T8498	T8499	det	the,organization
R1290	T8325	T8324	punct	-,order
R1291	T8499	T8496	nsubjpass	organization,modified
R1292	T8500	T8499	amod	nuclear,organization
R1293	T8501	T8499	prep	of,organization
R1294	T8326	T8322	dobj	structures,form
R1295	T8502	T8501	pobj	heterochromatin,of
R1296	T8503	T8496	auxpass	is,modified
R1297	T8504	T8496	advmod	dramatically,modified
R1298	T8505	T8496	prep	during,modified
R1299	T8506	T8507	amod	pre-implantation,development
R1300	T8507	T8505	pobj	development,during
R1301	T8327	T8326	compound	chromatin,structures
R1302	T8508	T8496	punct	", ",modified
R1303	T8509	T8496	prep	between,modified
R1304	T8510	T8511	det	the,stages
R1305	T8538	T8534	conj	16,eight
R1306	T8511	T8509	pobj	stages,between
R1307	T8512	T8513	nummod	two,cell
R1308	T8513	T8511	nmod	cell,stages
R1309	T8514	T8513	punct	-,cell
R1310	T8515	T8513	cc	and,cell
R1311	T8539	T8535	punct	-,cell
R1312	T8516	T8513	conj	blastocyst,cell
R1313	T8517	T8496	punct	.,modified
R1314	T8540	T8520	punct	", ",were
R1315	T8519	T8520	prep	In,were
R1316	T8521	T8522	nmod	Chaf1a,embryos
R1317	T8541	T8542	npadvmod	DAPI,dense
R1318	T8522	T8519	pobj	embryos,In
R1319	T8523	T8521	punct	−,Chaf1a
R1320	T8542	T8544	amod	dense,foci
R1321	T8524	T8521	punct	/,Chaf1a
R1322	T8525	T8521	punct	−,Chaf1a
R1323	T8526	T8522	compound	E4,embryos
R1324	T8543	T8542	punct	-,dense
R1325	T8527	T8522	punct	", ",embryos
R1326	T8528	T8529	dep	which,arrested
R1327	T8529	T8522	relcl	arrested,embryos
R1328	T8544	T8520	nsubj	foci,were
R1329	T8530	T8529	auxpass	are,arrested
R1330	T8531	T8529	prep	between,arrested
R1331	T8532	T8533	det	the,stage
R1332	T8545	T8546	advmod	barely,detectable
R1333	T8533	T8531	pobj	stage,between
R1334	T8534	T8535	nummod	eight,cell
R1335	T8535	T8533	compound	cell,stage
R1336	T8546	T8520	acomp	detectable,were
R1337	T8536	T8534	punct	-,eight
R1338	T8537	T8534	cc	and,eight
R1339	T8547	T8548	punct	(,1D
R1340	T8548	T8520	parataxis	1D,were
R1341	T8549	T8548	compound	Figure,1D
R1342	T8550	T8548	punct	),1D
R1343	T8643	T8642	punct	-,type
R1344	T8551	T8520	punct	.,were
R1345	T8644	T8645	punct	(,compare
R1346	T8645	T8617	parataxis	compare,is
R1347	T8553	T8554	advmod	Instead,was
R1348	T8646	T8647	compound	Figure,1D
R1349	T8647	T8645	dobj	1D,compare
R1350	T8648	T8647	cc	and,1D
R1351	T8649	T8647	conj	1F,1D
R1352	T8650	T8645	punct	),compare
R1353	T8651	T8617	punct	.,is
R1354	T8653	T8654	det	These,data
R1355	T8654	T8655	nsubj	data,evidence
R1356	T8656	T8657	det	a,role
R1357	T8657	T8655	dobj	role,evidence
R1358	T8555	T8554	punct	", ",was
R1359	T8658	T8657	amod	key,role
R1360	T8659	T8657	prep	for,role
R1361	T8660	T8659	pobj	p150CAF,for
R1362	T8661	T8660	punct	-,p150CAF
R1363	T8556	T8557	compound	DAPI,staining
R1364	T8662	T8660	nummod	1,p150CAF
R1365	T8663	T8655	prep	during,evidence
R1366	T8664	T8665	amod	pre-implantation,development
R1367	T8665	T8663	pobj	development,during
R1368	T8557	T8554	nsubj	staining,was
R1369	T8666	T8655	punct	", ",evidence
R1370	T8667	T8655	cc	and,evidence
R1371	T8668	T8655	conj	reveal,evidence
R1372	T8558	T8554	acomp	diffuse,was
R1373	T8669	T8670	mark	that,required
R1374	T8670	T8668	ccomp	required,reveal
R1375	T8671	T8672	det	this,protein
R1376	T8559	T8554	prep	within,was
R1377	T8672	T8670	nsubjpass	protein,required
R1378	T8673	T8670	auxpass	is,required
R1379	T8674	T8670	prep	for,required
R1380	T8675	T8676	det	the,organization
R1381	T8560	T8561	det	the,nucleus
R1382	T8676	T8674	pobj	organization,for
R1383	T8677	T8676	amod	proper,organization
R1384	T8678	T8679	nummod	3,D
R1385	T8561	T8559	pobj	nucleus,within
R1386	T8679	T8676	compound	D,organization
R1387	T8680	T8679	punct	-,D
R1388	T8681	T8676	prep	of,organization
R1389	T8562	T8554	punct	", ",was
R1390	T8682	T8681	pobj	heterochromatin,of
R1391	T8563	T8554	prep	with,was
R1392	T8683	T8670	prep	within,required
R1393	T8564	T8563	pobj	regions,with
R1394	T8684	T8685	amod	embryonic,nuclei
R1395	T8685	T8683	pobj	nuclei,within
R1396	T8686	T8685	compound	cell,nuclei
R1397	T8565	T8564	prep	of,regions
R1398	T8687	T8655	punct	.,evidence
R1399	T8689	T8690	advmod	Next,wondered
R1400	T8566	T8567	amod	higher,density
R1401	T8691	T8690	punct	", ",wondered
R1402	T8692	T8690	nsubj	we,wondered
R1403	T8693	T8694	mark	whether,observed
R1404	T8694	T8690	ccomp	observed,wondered
R1405	T8695	T8696	det	a,requirement
R1406	T8696	T8694	nsubjpass	requirement,observed
R1407	T8567	T8565	pobj	density,of
R1408	T8697	T8696	amod	similar,requirement
R1409	T8698	T8696	prep	for,requirement
R1410	T8568	T8567	prep	around,density
R1411	T8699	T8698	pobj	CAF,for
R1412	T8700	T8699	punct	-,CAF
R1413	T8701	T8699	nummod	1,CAF
R1414	T8702	T8694	aux	could,observed
R1415	T8569	T8570	det	the,nucleoli
R1416	T8703	T8694	advmod	also,observed
R1417	T8704	T8694	auxpass	be,observed
R1418	T8705	T8694	prep	in,observed
R1419	T8570	T8568	pobj	nucleoli,around
R1420	T8706	T8707	compound	ES,cells
R1421	T8707	T8705	pobj	cells,in
R1422	T8708	T8707	punct	", ",cells
R1423	T8571	T8568	cc	and,around
R1424	T8709	T8710	dep	which,derived
R1425	T8710	T8707	relcl	derived,cells
R1426	T8711	T8710	auxpass	are,derived
R1427	T8712	T8710	prep	from,derived
R1428	T8572	T8568	conj	at,around
R1429	T8713	T8714	det	the,mass
R1430	T8714	T8712	pobj	mass,from
R1431	T8715	T8714	nmod	blastocyst,mass
R1432	T8716	T8717	amod	inner,cell
R1433	T8573	T8574	det	the,periphery
R1434	T8717	T8714	compound	cell,mass
R1435	T8718	T8690	punct	.,wondered
R1436	T8574	T8572	pobj	periphery,at
R1437	T8720	T8721	prep	Given,derived
R1438	T8722	T8723	det	the,arrest
R1439	T8575	T8574	prep	of,periphery
R1440	T8723	T8720	pobj	arrest,Given
R1441	T8724	T8723	amod	early,arrest
R1442	T8725	T8723	amod	developmental,arrest
R1443	T8576	T8577	det	the,nuclei
R1444	T8726	T8723	acl	observed,arrest
R1445	T8727	T8726	prep	in,observed
R1446	T8728	T8729	nmod	Chaf1a,embryos
R1447	T8577	T8575	pobj	nuclei,of
R1448	T8729	T8727	pobj	embryos,in
R1449	T8730	T8728	punct	−,Chaf1a
R1450	T8731	T8728	punct	/,Chaf1a
R1451	T8578	T8579	punct	(,1D
R1452	T8732	T8728	punct	−,Chaf1a
R1453	T8733	T8721	punct	", ",derived
R1454	T8734	T8735	amod	such,cells
R1455	T8735	T8721	nsubjpass	cells,derived
R1456	T8736	T8721	aux	could,derived
R1457	T8737	T8721	neg	not,derived
R1458	T8738	T8721	auxpass	be,derived
R1459	T8579	T8554	parataxis	1D,was
R1460	T8739	T8721	advmod	directly,derived
R1461	T8740	T8721	prep	from,derived
R1462	T8741	T8742	amod	null,embryos
R1463	T8580	T8579	compound	Figure,1D
R1464	T8742	T8740	pobj	embryos,from
R1465	T8743	T8721	punct	", ",derived
R1466	T8744	T8721	cc	and,derived
R1467	T8581	T8579	punct	),1D
R1468	T8745	T8746	nsubj	we,used
R1469	T8746	T8721	conj	used,derived
R1470	T8747	T8746	advmod	thus,used
R1471	T8748	T8749	det	an,strategy
R1472	T8582	T8554	punct	.,was
R1473	T8584	T8585	nsubj	Localization,showed
R1474	T8586	T8584	prep	of,Localization
R1475	T8749	T8746	dobj	strategy,used
R1476	T8750	T8749	compound	RNAi,strategy
R1477	T8751	T8752	punct	(,2A
R1478	T8587	T8586	pobj	HP1α,of
R1479	T8752	T8746	parataxis	2A,used
R1480	T8753	T8752	compound	Figure,2A
R1481	T8754	T8752	punct	),2A
R1482	T8755	T8721	punct	.,derived
R1483	T8588	T8589	det	a,pattern
R1484	T8757	T8758	nmod	p150CAF,knockdown
R1485	T8758	T8761	nsubjpass	knockdown,quantified
R1486	T8589	T8585	dobj	pattern,showed
R1487	T8759	T8757	punct	-,p150CAF
R1488	T8760	T8757	nummod	1,p150CAF
R1489	T8590	T8589	amod	diffuse,pattern
R1490	T8762	T8758	prep	in,knockdown
R1491	T8763	T8764	compound	ES,cells
R1492	T8764	T8762	pobj	cells,in
R1493	T8591	T8589	amod	similar,pattern
R1494	T8765	T8761	auxpass	was,quantified
R1495	T8766	T8761	prep	by,quantified
R1496	T8592	T8591	prep	to,similar
R1497	T8767	T8768	compound	Western,blot
R1498	T8768	T8769	compound	blot,analysis
R1499	T8769	T8766	pobj	analysis,by
R1500	T8593	T8594	det	the,DAPI
R1501	T8594	T8592	pobj	DAPI,to
R1502	T8770	T8769	cc	and,analysis
R1503	T8595	T8589	punct	", ",pattern
R1504	T8771	T8769	conj	IF,analysis
R1505	T8772	T8761	punct	.,quantified
R1506	T8774	T8775	punct	(,S1
R1507	T8776	T8775	compound	Figure,S1
R1508	T8777	T8775	punct	),S1
R1509	T8596	T8589	prep	with,pattern
R1510	T8778	T8775	punct	.,S1
R1511	T8780	T8781	nmod	p150CAF,cells
R1512	T8597	T8598	det	some,enrichment
R1513	T8781	T8788	nsubj	cells,displayed
R1514	T8782	T8780	punct	-,p150CAF
R1515	T8598	T8596	pobj	enrichment,with
R1516	T8783	T8780	nummod	1,p150CAF
R1517	T8784	T8785	npadvmod	RNAi,depleted
R1518	T8785	T8781	amod	depleted,cells
R1519	T8599	T8598	prep	at,enrichment
R1520	T8786	T8785	punct	-,depleted
R1521	T8787	T8781	compound	ES,cells
R1522	T8600	T8601	det	the,periphery
R1523	T8789	T8790	det	a,phenotype
R1524	T8790	T8788	dobj	phenotype,displayed
R1525	T8791	T8792	advmod	very,similar
R1526	T8792	T8790	amod	similar,phenotype
R1527	T8601	T8599	pobj	periphery,at
R1528	T8793	T8792	prep	to,similar
R1529	T8794	T8795	det	the,cells
R1530	T8795	T8793	pobj	cells,to
R1531	T8602	T8601	amod	nuclear,periphery
R1532	T8796	T8795	prep	of,cells
R1533	T8797	T8798	nmod	Chaf1a,mice
R1534	T8798	T8796	pobj	mice,of
R1535	T8799	T8797	punct	−,Chaf1a
R1536	T8603	T8599	punct	", ",at
R1537	T8800	T8797	punct	/,Chaf1a
R1538	T8801	T8797	punct	−,Chaf1a
R1539	T8802	T8798	compound	E4,mice
R1540	T8604	T8599	cc	and,at
R1541	T8803	T8788	punct	.,displayed
R1542	T8805	T8806	npadvmod	DAPI,dense
R1543	T8605	T8599	conj	around,at
R1544	T8806	T8808	amod	dense,foci
R1545	T8807	T8806	punct	-,dense
R1546	T8808	T8809	nsubjpass	foci,lost
R1547	T8606	T8607	det	the,nucleoli
R1548	T8810	T8809	auxpass	were,lost
R1549	T8811	T8809	punct	", ",lost
R1550	T8812	T8809	cc	and,lost
R1551	T8607	T8605	pobj	nucleoli,around
R1552	T8813	T8814	nsubj	we,observed
R1553	T8814	T8809	conj	observed,lost
R1554	T8815	T8816	amod	diffuse,staining
R1555	T8608	T8609	punct	(,1D
R1556	T8816	T8814	dobj	staining,observed
R1557	T8817	T8816	nmod	HP1α,staining
R1558	T8818	T8817	cc	and,HP1α
R1559	T8819	T8817	conj	DAPI,HP1α
R1560	T8820	T8814	prep	around,observed
R1561	T8821	T8822	det	the,nucleoli
R1562	T8822	T8820	pobj	nucleoli,around
R1563	T8609	T8585	parataxis	1D,showed
R1564	T8823	T8820	cc	and,around
R1565	T8824	T8820	conj	at,around
R1566	T8610	T8609	compound	Figure,1D
R1567	T8825	T8826	det	the,periphery
R1568	T8826	T8824	pobj	periphery,at
R1569	T8827	T8826	prep	of,periphery
R1570	T8828	T8829	det	the,nucleus
R1571	T8829	T8827	pobj	nucleus,of
R1572	T8611	T8609	punct	),1D
R1573	T8830	T8831	punct	(,2B
R1574	T8831	T8814	parataxis	2B,observed
R1575	T8832	T8831	compound	Figure,2B
R1576	T8612	T8585	punct	.,showed
R1577	T8833	T8831	punct	),2B
R1578	T8834	T8814	punct	.,observed
R1579	T8614	T8615	det	This,organization
R1580	T8836	T8837	det	This,result
R1581	T8837	T8838	nsubj	result,indicates
R1582	T8839	T8840	mark	that,is
R1583	T8615	T8617	nsubj	organization,is
R1584	T8840	T8838	ccomp	is,indicates
R1585	T8841	T8840	nsubj	p150CAF,is
R1586	T8842	T8841	punct	-,p150CAF
R1587	T8616	T8615	amod	abnormal,organization
R1588	T8843	T8841	nummod	1,p150CAF
R1589	T8844	T8840	acomp	essential,is
R1590	T8845	T8844	prep	for,essential
R1591	T8618	T8615	prep	of,organization
R1592	T8846	T8847	amod	nuclear,organization
R1593	T8847	T8845	pobj	organization,for
R1594	T8848	T8847	prep	of,organization
R1595	T8849	T8848	pobj	heterochromatin,of
R1596	T8619	T8618	pobj	heterochromatin,of
R1597	T8850	T8849	prep	in,heterochromatin
R1598	T8851	T8852	compound	ES,cells
R1599	T8852	T8850	pobj	cells,in
R1600	T8620	T8615	prep	in,organization
R1601	T8853	T8847	prep	in,organization
R1602	T8621	T8622	nmod	Chaf1a,embryos
R1603	T8622	T8620	pobj	embryos,in
R1604	T8854	T8855	det	a,way
R1605	T8623	T8621	punct	−,Chaf1a
R1606	T8855	T8853	pobj	way,in
R1607	T8856	T8855	amod	similar,way
R1608	T8857	T8855	prep	to,way
R1609	T8624	T8621	punct	/,Chaf1a
R1610	T8858	T8859	amod	early,embryos
R1611	T8859	T8857	pobj	embryos,to
R1612	T8860	T8859	amod	pre-implantation,embryos
R1613	T8861	T8838	punct	.,indicates
R1614	T8625	T8621	punct	−,Chaf1a
R1615	T8863	T8864	advmod	Surprisingly,observe
R1616	T8626	T8617	acomp	reminiscent,is
R1617	T8865	T8864	punct	", ",observe
R1618	T8866	T8864	nsubj	we,observe
R1619	T8867	T8864	aux	did,observe
R1620	T8868	T8864	neg	not,observe
R1621	T8627	T8626	prep	of,reminiscent
R1622	T8869	T8870	det	this,alteration
R1623	T8628	T8629	det	the,organization
R1624	T8870	T8864	dobj	alteration,observe
R1625	T8871	T8870	amod	severe,alteration
R1626	T8872	T8870	prep	in,alteration
R1627	T8873	T8874	compound	heterochromatin,organization
R1628	T8629	T8627	pobj	organization,of
R1629	T8874	T8872	pobj	organization,in
R1630	T8875	T8864	prep	in,observe
R1631	T8876	T8877	amod	primary,fibroblasts
R1632	T8630	T8629	compound	heterochromatin,organization
R1633	T8877	T8875	pobj	fibroblasts,in
R1634	T8878	T8877	nmod	mouse,fibroblasts
R1635	T8879	T8877	amod	embryonic,fibroblasts
R1636	T8880	T8877	punct	(,fibroblasts
R1637	T8631	T8629	acl	found,organization
R1638	T8881	T8877	appos	MEFs,fibroblasts
R1639	T8882	T8864	punct	),observe
R1640	T8883	T8864	prep	following,observe
R1641	T8632	T8631	prep	in,found
R1642	T8884	T8885	nmod	p150CAF,depletion
R1643	T8885	T8883	pobj	depletion,following
R1644	T8886	T8884	punct	-,p150CAF
R1645	T8633	T8634	quantmod	two,four
R1646	T8887	T8884	nummod	1,p150CAF
R1647	T8888	T8885	prep	by,depletion
R1648	T8889	T8888	pobj	RNAi,by
R1649	T8634	T8637	nummod	four,cell
R1650	T8890	T8864	punct	.,observe
R1651	T8892	T8893	nmod	p150CAF,depletion
R1652	T8635	T8634	punct	-,four
R1653	T8893	T8896	nsubj	depletion,resulted
R1654	T8894	T8892	punct	-,p150CAF
R1655	T8895	T8892	nummod	1,p150CAF
R1656	T8636	T8634	quantmod	to,four
R1657	T8897	T8893	prep	in,depletion
R1658	T8898	T8897	pobj	MEFs,in
R1659	T8637	T8639	nmod	cell,stage
R1660	T8899	T8896	prep	in,resulted
R1661	T8900	T8901	det	a,inhibition
R1662	T8901	T8899	pobj	inhibition,in
R1663	T8902	T8901	amod	strong,inhibition
R1664	T8903	T8901	prep	of,inhibition
R1665	T8904	T8905	compound	cell,proliferation
R1666	T8905	T8903	pobj	proliferation,of
R1667	T8638	T8637	punct	-,cell
R1668	T8906	T8907	punct	(,data
R1669	T8907	T8896	meta	data,resulted
R1670	T8908	T8907	amod	unpublished,data
R1671	T8909	T8907	punct	),data
R1672	T8639	T8640	nmod	stage,embryos
R1673	T8910	T8896	punct	", ",resulted
R1674	T8911	T8896	cc	but,resulted
R1675	T8912	T8913	aux	did,alter
R1676	T8913	T8896	conj	alter,resulted
R1677	T8640	T8632	pobj	embryos,in
R1678	T8914	T8913	neg	not,alter
R1679	T8915	T8916	det	the,clustering
R1680	T8916	T8913	dobj	clustering,alter
R1681	T8641	T8642	amod	wild,type
R1682	T8917	T8916	prep	of,clustering
R1683	T8918	T8919	compound	heterochromatin,domains
R1684	T8919	T8917	pobj	domains,of
R1685	T8642	T8640	compound	type,embryos
R1686	T8920	T8921	punct	(,2C
R1687	T8921	T8913	parataxis	2C,alter
R1688	T8922	T8921	compound	Figures,2C
R1689	T8959	T8957	pobj	embryo,in
R1690	T8923	T8921	cc	and,2C
R1691	T8924	T8921	conj	S2,2C
R1692	T8925	T8921	punct	),2C
R1693	T8926	T8896	punct	.,resulted
R1694	T8928	T8929	advmod	Similarly,affect
R1695	T8960	T8959	amod	early,embryo
R1696	T8930	T8929	punct	", ",affect
R1697	T8931	T8932	nmod	p150CAF,depletion
R1698	T8961	T8959	cc	and,embryo
R1699	T8932	T8929	nsubj	depletion,affect
R1700	T8933	T8931	punct	-,p150CAF
R1701	T8962	T8963	compound	ES,cells
R1702	T8934	T8931	nummod	1,p150CAF
R1703	T8935	T8932	prep	in,depletion
R1704	T8936	T8937	compound	3T3,cells
R1705	T8937	T8935	pobj	cells,in
R1706	T8963	T8959	conj	cells,embryo
R1707	T8938	T8929	aux	did,affect
R1708	T8939	T8929	neg	not,affect
R1709	T8940	T8941	compound	heterochromatin,organization
R1710	T8964	T8949	punct	.,reveal
R1711	T8941	T8929	dobj	organization,affect
R1712	T8942	T8943	punct	[,16
R1713	T8943	T8929	parataxis	16,affect
R1714	T8944	T8943	punct	],16
R1715	T8945	T8929	punct	.,affect
R1716	T8966	T8967	nsubj	We,wondered
R1717	T8947	T8948	det	These,observations
R1718	T8948	T8949	nsubj	observations,reveal
R1719	T8950	T8951	det	a,function
R1720	T8968	T8967	advmod	also,wondered
R1721	T8951	T8949	dobj	function,reveal
R1722	T8952	T8951	amod	specific,function
R1723	T8953	T8951	prep	for,function
R1724	T8954	T8953	pobj	CAF,for
R1725	T8955	T8954	punct	-,CAF
R1726	T8956	T8954	nummod	1,CAF
R1727	T8969	T8970	mark	whether,affects
R1728	T8957	T8951	prep	in,function
R1729	T8958	T8959	det	the,embryo
R1730	T8970	T8967	advcl	affects,wondered
R1731	T8971	T8970	nsubj	loss,affects
R1732	T8972	T8971	prep	of,loss
R1733	T8973	T8972	pobj	p150CAF,of
R1734	T9065	T9064	compound	Figure,2D
R1735	T8974	T8973	punct	-,p150CAF
R1736	T9066	T9064	punct	),2D
R1737	T9067	T9038	punct	.,detect
R1738	T8975	T8973	nummod	1,p150CAF
R1739	T9069	T9070	det	These,data
R1740	T9070	T9071	nsubj	data,show
R1741	T8976	T8971	prep	in,loss
R1742	T9072	T9073	mark	that,altered
R1743	T9073	T9071	ccomp	altered,show
R1744	T8977	T8978	compound	ES,cells
R1745	T9074	T9075	amod	nuclear,architecture
R1746	T9075	T9073	nsubjpass	architecture,altered
R1747	T9076	T9073	auxpass	is,altered
R1748	T8978	T8976	pobj	cells,in
R1749	T9077	T9073	neg	not,altered
R1750	T9078	T9073	advmod	globally,altered
R1751	T8979	T8970	advmod	specifically,affects
R1752	T9079	T9073	prep	following,altered
R1753	T9080	T9079	pobj	CAF,following
R1754	T9081	T9080	punct	-,CAF
R1755	T8980	T8981	det	the,subcompartment
R1756	T9082	T9080	nummod	1,CAF
R1757	T9083	T9080	appos	loss,CAF
R1758	T9084	T9083	punct	-,loss
R1759	T8981	T8970	dobj	subcompartment,affects
R1760	T9085	T9083	prep	of,loss
R1761	T9086	T9085	punct	-,of
R1762	T9087	T9085	pobj	function,of
R1763	T8982	T8981	nmod	heterochromatin,subcompartment
R1764	T9088	T9071	punct	.,show
R1765	T9090	T9091	prep	In,revealed
R1766	T9092	T9090	pobj	addition,In
R1767	T9093	T9091	punct	", ",revealed
R1768	T9094	T9095	compound	Western,blot
R1769	T8983	T8981	amod	nuclear,subcompartment
R1770	T9095	T9096	compound	blot,analysis
R1771	T9096	T9091	nsubj	analysis,revealed
R1772	T9097	T9098	mark	that,result
R1773	T8984	T8970	punct	", ",affects
R1774	T9098	T9091	ccomp	result,revealed
R1775	T9099	T9100	nmod	CAF,depletion
R1776	T9100	T9098	nsubj	depletion,result
R1777	T8985	T8970	cc	or,affects
R1778	T9101	T9099	punct	-,CAF
R1779	T9102	T9099	nummod	1,CAF
R1780	T9103	T9098	aux	did,result
R1781	T8986	T8987	mark	whether,result
R1782	T9104	T9098	neg	not,result
R1783	T9105	T9098	prep	in,result
R1784	T9106	T9107	amod	altered,levels
R1785	T8987	T8970	conj	result,affects
R1786	T9107	T9105	pobj	levels,in
R1787	T9108	T9107	prep	of,levels
R1788	T9109	T9110	nmod	chromatin,proteins
R1789	T8988	T8987	nsubj	it,result
R1790	T9110	T9108	pobj	proteins,of
R1791	T9111	T9110	amod	architectural,proteins
R1792	T9112	T9113	amod	such,as
R1793	T9113	T9110	prep	as,proteins
R1794	T8989	T8987	aux	might,result
R1795	T9114	T9115	compound	histone,H3
R1796	T9115	T9113	pobj	H3,as
R1797	T9116	T9115	cc	and,H3
R1798	T8990	T8987	prep	in,result
R1799	T9117	T9115	conj	HP1α,H3
R1800	T9118	T9119	punct	(,S1
R1801	T8991	T8992	det	a,loss
R1802	T9119	T9091	parataxis	S1,revealed
R1803	T8992	T8990	pobj	loss,in
R1804	T9120	T9119	compound	Figure,S1
R1805	T9121	T9119	punct	),S1
R1806	T9122	T9091	punct	.,revealed
R1807	T8993	T8994	advmod	more,global
R1808	T9124	T9125	advmod	Thus,required
R1809	T8994	T8992	amod	global,loss
R1810	T9126	T9125	punct	", ",required
R1811	T9127	T9125	nsubjpass	CAF,required
R1812	T8995	T8992	prep	of,loss
R1813	T9128	T9127	punct	-,CAF
R1814	T9129	T9127	nummod	1,CAF
R1815	T9130	T9125	auxpass	is,required
R1816	T8996	T8997	amod	nuclear,organization
R1817	T9131	T9125	advmod	specifically,required
R1818	T9132	T9125	prep	in,required
R1819	T9133	T9134	amod	early,embryos
R1820	T9134	T9132	pobj	embryos,in
R1821	T9135	T9132	cc	and,in
R1822	T9136	T9132	conj	in,in
R1823	T8997	T8995	pobj	organization,of
R1824	T9137	T9138	compound	ES,cells
R1825	T9138	T9136	pobj	cells,in
R1826	T9139	T9125	prep	for,required
R1827	T8998	T8997	cc	and,organization
R1828	T9140	T9141	det	the,organization
R1829	T9141	T9139	pobj	organization,for
R1830	T9142	T9141	amod	proper,organization
R1831	T8999	T8997	conj	architecture,organization
R1832	T9143	T9141	prep	of,organization
R1833	T9144	T9145	det	the,compartment
R1834	T9145	T9143	pobj	compartment,of
R1835	T9000	T8967	punct	.,wondered
R1836	T9146	T9145	nmod	heterochromatin,compartment
R1837	T9147	T9145	amod	subnuclear,compartment
R1838	T9148	T9125	punct	.,required
R1839	T9002	T9003	nsubj	We,used
R1840	T9150	T9151	amod	Previous,studies
R1841	T9151	T9152	nsubj	studies,showed
R1842	T9004	T9005	det	a,antibody
R1843	T9153	T9151	acl	performed,studies
R1844	T9154	T9153	prep	in,performed
R1845	T9155	T9156	amod	mammalian,lines
R1846	T9156	T9154	pobj	lines,in
R1847	T9157	T9156	compound	cell,lines
R1848	T9005	T9003	dobj	antibody,used
R1849	T9158	T9159	mark	that,required
R1850	T9159	T9152	ccomp	required,showed
R1851	T9160	T9161	nmod	CAF,activity
R1852	T9161	T9159	nsubjpass	activity,required
R1853	T9006	T9005	amod	specific,antibody
R1854	T9162	T9160	punct	-,CAF
R1855	T9163	T9160	nummod	1,CAF
R1856	T9164	T9159	auxpass	is,required
R1857	T9007	T9005	prep	against,antibody
R1858	T9165	T9159	prep	for,required
R1859	T9166	T9167	compound	S,phase
R1860	T9008	T9009	det	the,protein
R1861	T9167	T9168	compound	phase,progression
R1862	T9168	T9165	pobj	progression,for
R1863	T9169	T9170	punct	[,24
R1864	T9009	T9007	pobj	protein,against
R1865	T9170	T9152	parataxis	24,showed
R1866	T9010	T9011	amod	promyelocytic,leukemia
R1867	T9011	T9009	nmod	leukemia,protein
R1868	T9171	T9170	nummod	16,24
R1869	T9172	T9170	punct	",",24
R1870	T9173	T9170	nummod	23,24
R1871	T9174	T9170	punct	",",24
R1872	T9175	T9170	punct	],24
R1873	T9012	T9011	punct	(,leukemia
R1874	T9176	T9152	punct	.,showed
R1875	T9178	T9179	nsubj	We,show
R1876	T9013	T9011	appos	PML,leukemia
R1877	T9180	T9179	advmod	here,show
R1878	T9181	T9182	mark	that,appear
R1879	T9014	T9009	punct	),protein
R1880	T9015	T9016	aux	to,study
R1881	T9182	T9179	ccomp	appear,show
R1882	T9183	T9184	nummod	3,d
R1883	T9184	T9185	npadvmod	d,after
R1884	T9016	T9003	advcl	study,used
R1885	T9185	T9182	prep	after,appear
R1886	T9186	T9185	pobj	transfection,after
R1887	T9017	T9018	det	the,fate
R1888	T9187	T9186	prep	of,transfection
R1889	T9188	T9189	det	the,vector
R1890	T9189	T9187	pobj	vector,of
R1891	T9018	T9016	dobj	fate,study
R1892	T9190	T9189	compound	RNAi,vector
R1893	T9191	T9182	punct	", ",appear
R1894	T9192	T9193	npadvmod	p150CAF,depleted
R1895	T9019	T9018	prep	of,fate
R1896	T9193	T9197	amod	depleted,cells
R1897	T9194	T9192	punct	-,p150CAF
R1898	T9195	T9192	nummod	1,p150CAF
R1899	T9020	T9021	det	the,bodies
R1900	T9196	T9193	punct	-,depleted
R1901	T9197	T9182	nsubj	cells,appear
R1902	T9198	T9197	punct	(,cells
R1903	T9021	T9019	pobj	bodies,of
R1904	T9199	T9197	acl	identified,cells
R1905	T9200	T9199	agent	by,identified
R1906	T9201	T9202	det	a,absence
R1907	T9022	T9021	nmod	PML,bodies
R1908	T9202	T9200	pobj	absence,by
R1909	T9203	T9202	amod	complete,absence
R1910	T9204	T9202	prep	of,absence
R1911	T9023	T9021	amod	nuclear,bodies
R1912	T9205	T9206	nmod	CAF,signal
R1913	T9206	T9204	pobj	signal,of
R1914	T9207	T9205	punct	-,CAF
R1915	T9024	T9021	punct	", ",bodies
R1916	T9208	T9205	nummod	1,CAF
R1917	T9209	T9206	compound	IF,signal
R1918	T9210	T9197	punct	),cells
R1919	T9025	T9026	dep	which,are
R1920	T9211	T9212	advmod	still,active
R1921	T9212	T9182	oprd	active,appear
R1922	T9213	T9212	prep	for,active
R1923	T9214	T9213	pobj	replication,for
R1924	T9215	T9182	punct	", ",appear
R1925	T9216	T9217	mark	as,revealed
R1926	T9026	T9021	relcl	are,bodies
R1927	T9217	T9182	advcl	revealed,appear
R1928	T9218	T9217	agent	by,revealed
R1929	T9219	T9218	pobj	incorporation,by
R1930	T9027	T9028	advmod	well,characterized
R1931	T9220	T9219	prep	of,incorporation
R1932	T9221	T9222	det	the,analog
R1933	T9028	T9030	amod	characterized,structures
R1934	T9222	T9220	pobj	analog,of
R1935	T9223	T9222	compound	thymidine,analog
R1936	T9224	T9222	appos	bromodeoxyuridine,analog
R1937	T9225	T9224	punct	(,bromodeoxyuridine
R1938	T9029	T9028	punct	-,characterized
R1939	T9226	T9224	appos	BrdU,bromodeoxyuridine
R1940	T9227	T9219	punct	),incorporation
R1941	T9228	T9229	punct	(,3A
R1942	T9030	T9026	attr	structures,are
R1943	T9229	T9219	parataxis	3A,incorporation
R1944	T9230	T9229	compound	Figure,3A
R1945	T9031	T9030	amod	subnuclear,structures
R1946	T9231	T9229	punct	),3A
R1947	T9232	T9219	punct	", ",incorporation
R1948	T9233	T9234	det	the,pattern
R1949	T9032	T9033	punct	[,22
R1950	T9234	T9219	conj	pattern,incorporation
R1951	T9235	T9234	nmod	PCNA,pattern
R1952	T9236	T9235	punct	(,PCNA
R1953	T9033	T9003	parataxis	22,used
R1954	T9237	T9238	amod	proliferating,antigen
R1955	T9238	T9235	appos	antigen,PCNA
R1956	T9239	T9238	nmod	cell,antigen
R1957	T9034	T9033	punct	],22
R1958	T9240	T9238	amod	nuclear,antigen
R1959	T9241	T9234	punct	),pattern
R1960	T9242	T9243	punct	(,3B
R1961	T9035	T9003	punct	.,used
R1962	T9243	T9234	parataxis	3B,pattern
R1963	T9244	T9243	compound	Figure,3B
R1964	T9245	T9243	punct	),3B
R1965	T9246	T9234	punct	", ",pattern
R1966	T9037	T9038	nsubj	We,detect
R1967	T9247	T9234	cc	and,pattern
R1968	T9248	T9249	compound	flow,analysis
R1969	T9249	T9234	conj	analysis,pattern
R1970	T9250	T9249	compound	cytometry,analysis
R1971	T9039	T9038	aux	could,detect
R1972	T9251	T9252	punct	(,3C
R1973	T9252	T9249	parataxis	3C,analysis
R1974	T9253	T9252	compound	Figure,3C
R1975	T9254	T9252	punct	),3C
R1976	T9255	T9179	punct	.,show
R1977	T9257	T9258	advmod	Hence,persists
R1978	T9259	T9258	punct	", ",persists
R1979	T9040	T9038	neg	not,detect
R1980	T9260	T9258	prep	in,persists
R1981	T9261	T9262	det	these,cells
R1982	T9262	T9260	pobj	cells,in
R1983	T9263	T9258	punct	", ",persists
R1984	T9041	T9042	det	any,difference
R1985	T9264	T9265	compound	DNA,replication
R1986	T9265	T9258	nsubj	replication,persists
R1987	T9266	T9258	prep	despite,persists
R1988	T9042	T9038	dobj	difference,detect
R1989	T9267	T9268	det	the,changes
R1990	T9268	T9266	pobj	changes,despite
R1991	T9269	T9268	amod	drastic,changes
R1992	T9043	T9042	amod	significant,difference
R1993	T9270	T9268	prep	in,changes
R1994	T9271	T9272	compound	heterochromatin,organization
R1995	T9272	T9270	pobj	organization,in
R1996	T9044	T9042	prep	in,difference
R1997	T9273	T9258	punct	.,persists
R1998	T9275	T9276	advmod	However,cease
R1999	T9045	T9046	det	the,distribution
R2000	T9046	T9044	pobj	distribution,in
R2001	T9047	T9046	cc	and,distribution
R2002	T9277	T9276	punct	", ",cease
R2003	T9048	T9046	conj	aspect,distribution
R2004	T9278	T9279	nummod	24,h
R2005	T9279	T9280	npadvmod	h,later
R2006	T9280	T9276	advmod	later,cease
R2007	T9049	T9046	prep	of,distribution
R2008	T9281	T9276	punct	", ",cease
R2009	T9282	T9283	compound	ES,cells
R2010	T9283	T9276	nsubj	cells,cease
R2011	T9284	T9285	aux	to,proliferate
R2012	T9050	T9051	nmod	PML,bodies
R2013	T9285	T9276	xcomp	proliferate,cease
R2014	T9286	T9276	cc	and,cease
R2015	T9051	T9049	pobj	bodies,of
R2016	T9287	T9276	conj	die,cease
R2017	T9288	T9276	punct	", ",cease
R2018	T9289	T9276	advcl	revealing,cease
R2019	T9290	T9291	mark	that,required
R2020	T9052	T9051	amod	nuclear,bodies
R2021	T9053	T9051	prep	between,bodies
R2022	T9291	T9289	ccomp	required,revealing
R2023	T9292	T9291	nsubjpass	p150CAF,required
R2024	T9054	T9055	nmod	control,cells
R2025	T9293	T9292	punct	-,p150CAF
R2026	T9294	T9292	nummod	1,p150CAF
R2027	T9295	T9291	auxpass	is,required
R2028	T9296	T9291	prep	for,required
R2029	T9297	T9298	det	an,process
R2030	T9298	T9296	pobj	process,for
R2031	T9299	T9298	amod	essential,process
R2032	T9055	T9053	pobj	cells,between
R2033	T9300	T9298	amod	cellular,process
R2034	T9301	T9291	punct	", ",required
R2035	T9302	T9291	prep	as,required
R2036	T9056	T9054	cc	and,control
R2037	T9303	T9302	prep	in,as
R2038	T9304	T9303	pobj	embryos,in
R2039	T9305	T9276	punct	.,cease
R2040	T9057	T9058	npadvmod	p150CAF,depleted
R2041	T9307	T9308	advmod	Altogether,demonstrate
R2042	T9058	T9054	conj	depleted,control
R2043	T9309	T9308	punct	", ",demonstrate
R2044	T9310	T9311	poss	our,data
R2045	T9059	T9057	punct	-,p150CAF
R2046	T9311	T9308	nsubj	data,demonstrate
R2047	T9312	T9313	mark	that,alters
R2048	T9313	T9308	ccomp	alters,demonstrate
R2049	T9060	T9057	nummod	1,p150CAF
R2050	T9314	T9313	nsubj	loss,alters
R2051	T9315	T9314	prep	of,loss
R2052	T9316	T9317	nmod	p150CAF,function
R2053	T9061	T9058	punct	-,depleted
R2054	T9317	T9315	pobj	function,of
R2055	T9318	T9316	punct	-,p150CAF
R2056	T9319	T9316	nummod	1,p150CAF
R2057	T9062	T9055	compound	ES,cells
R2058	T9320	T9314	prep	in,loss
R2059	T9321	T9322	compound	ES,cells
R2060	T9322	T9320	pobj	cells,in
R2061	T9063	T9064	punct	(,2D
R2062	T9323	T9322	cc	and,cells
R2063	T9324	T9325	amod	early,embryos
R2064	T9325	T9322	conj	embryos,cells
R2065	T9064	T9038	parataxis	2D,detect
R2066	T9326	T9313	advmod	first,alters
R2067	T9327	T9328	det	the,organization
R2068	T9328	T9313	dobj	organization,alters
R2069	T9382	T9379	amod	chromosomal,regions
R2070	T9329	T9328	prep	of,organization
R2071	T9330	T9329	pobj	heterochromatin,of
R2072	T9331	T9328	prep	in,organization
R2073	T9332	T9333	det	the,nucleus
R2074	T9333	T9331	pobj	nucleus,in
R2075	T9334	T9313	cc	and,alters
R2076	T9383	T9379	punct	", ",regions
R2077	T9335	T9313	punct	", ",alters
R2078	T9336	T9337	prep	in,cycle
R2079	T9337	T9313	conj	cycle,alters
R2080	T9338	T9339	det	a,step
R2081	T9339	T9336	pobj	step,in
R2082	T9340	T9339	amod	subsequent,step
R2083	T9341	T9337	punct	", ",cycle
R2084	T9342	T9337	compound	cell,cycle
R2085	T9384	T9385	dep	which,composed
R2086	T9343	T9337	cc	and,cycle
R2087	T9344	T9337	conj	viability,cycle
R2088	T9345	T9308	punct	.,demonstrate
R2089	T9385	T9379	relcl	composed,regions
R2090	T9347	T9348	prep	In,performed
R2091	T9349	T9347	pobj	order,In
R2092	T9350	T9351	aux	to,characterize
R2093	T9386	T9385	prep	in,composed
R2094	T9351	T9349	acl	characterize,order
R2095	T9352	T9351	advmod	better,characterize
R2096	T9353	T9354	det	the,defects
R2097	T9387	T9386	pobj	mouse,in
R2098	T9354	T9351	dobj	defects,characterize
R2099	T9355	T9354	prep	in,defects
R2100	T9388	T9385	auxpass	are,composed
R2101	T9389	T9385	advmod	mainly,composed
R2102	T9356	T9357	compound	heterochromatin,organization
R2103	T9390	T9385	prep	of,composed
R2104	T9357	T9355	pobj	organization,in
R2105	T9358	T9348	punct	", ",performed
R2106	T9359	T9348	nsubj	we,performed
R2107	T9391	T9392	amod	large,blocks
R2108	T9360	T9361	nummod	two,color
R2109	T9361	T9363	nmod	color,experiments
R2110	T9362	T9361	punct	-,color
R2111	T9392	T9390	pobj	blocks,of
R2112	T9363	T9348	dobj	experiments,performed
R2113	T9364	T9363	nmod	DNA,experiments
R2114	T9365	T9366	nmod	fluorescence,hybridization
R2115	T9393	T9392	prep	of,blocks
R2116	T9366	T9363	nmod	hybridization,experiments
R2117	T9367	T9368	advmod	in,situ
R2118	T9368	T9366	amod	situ,hybridization
R2119	T9369	T9366	punct	(,hybridization
R2120	T9394	T9395	amod	major,repeats
R2121	T9370	T9366	appos	FISH,hybridization
R2122	T9371	T9363	punct	),experiments
R2123	T9372	T9373	aux	to,reveal
R2124	T9395	T9393	pobj	repeats,of
R2125	T9373	T9348	advcl	reveal,performed
R2126	T9374	T9375	det	the,distribution
R2127	T9396	T9394	cc	and,major
R2128	T9375	T9373	dobj	distribution,reveal
R2129	T9376	T9375	amod	spatial,distribution
R2130	T9377	T9375	prep	of,distribution
R2131	T9378	T9379	amod	pericentric,regions
R2132	T9397	T9394	conj	minor,major
R2133	T9379	T9377	pobj	regions,of
R2134	T9380	T9378	cc	and,pericentric
R2135	T9381	T9378	conj	centric,pericentric
R2136	T9398	T9395	compound	satellite,repeats
R2137	T9399	T9385	punct	", ",composed
R2138	T9400	T9385	advmod	respectively,composed
R2139	T9401	T9402	punct	[,20
R2140	T9488	T9486	punct	),4B
R2141	T9489	T9459	punct	.,observed
R2142	T9402	T9348	parataxis	20,performed
R2143	T9491	T9492	amod	Individual,domains
R2144	T9403	T9402	punct	],20
R2145	T9492	T9494	nsubjpass	domains,found
R2146	T9493	T9492	amod	pericentric,domains
R2147	T9404	T9348	punct	.,performed
R2148	T9495	T9492	prep	from,domains
R2149	T9496	T9497	amod	single,chromosomes
R2150	T9497	T9495	pobj	chromosomes,from
R2151	T9498	T9494	auxpass	are,found
R2152	T9406	T9407	amod	Pericentric,domains
R2153	T9499	T9494	advmod	now,found
R2154	T9500	T9501	preconj	either,isolated
R2155	T9501	T9494	oprd	isolated,found
R2156	T9502	T9501	cc	or,isolated
R2157	T9407	T9408	nsubj	domains,form
R2158	T9503	T9501	conj	aggregated,isolated
R2159	T9504	T9501	prep	in,isolated
R2160	T9409	T9407	prep	from,domains
R2161	T9505	T9506	det	a,pattern
R2162	T9506	T9504	pobj	pattern,in
R2163	T9507	T9508	advmod	less,dense
R2164	T9508	T9506	amod	dense,pattern
R2165	T9410	T9411	amod	different,chromosomes
R2166	T9509	T9508	prep	than,dense
R2167	T9510	T9511	det	the,clusters
R2168	T9511	T9509	pobj	clusters,than
R2169	T9411	T9409	pobj	chromosomes,from
R2170	T9512	T9511	amod	regular,clusters
R2171	T9513	T9511	acl	observed,clusters
R2172	T9514	T9513	prep	in,observed
R2173	T9412	T9408	dobj	clusters,form
R2174	T9515	T9516	compound	control,cells
R2175	T9516	T9514	pobj	cells,in
R2176	T9517	T9518	punct	(,4B
R2177	T9413	T9412	punct	", ",clusters
R2178	T9518	T9494	parataxis	4B,found
R2179	T9519	T9518	compound	Figure,4B
R2180	T9520	T9518	punct	),4B
R2181	T9521	T9494	punct	.,found
R2182	T9523	T9524	amod	Such,aggregates
R2183	T9414	T9415	dep	which,revealed
R2184	T9524	T9525	nsubjpass	aggregates,revealed
R2185	T9526	T9524	punct	", ",aggregates
R2186	T9415	T9412	relcl	revealed,clusters
R2187	T9527	T9528	dep	which,found
R2188	T9528	T9524	relcl	found,aggregates
R2189	T9529	T9528	auxpass	are,found
R2190	T9416	T9415	auxpass	are,revealed
R2191	T9530	T9528	advmod	often,found
R2192	T9531	T9528	prep	at,found
R2193	T9532	T9533	det	the,periphery
R2194	T9417	T9415	prep	by,revealed
R2195	T9533	T9531	pobj	periphery,at
R2196	T9534	T9533	amod	nuclear,periphery
R2197	T9535	T9525	punct	", ",revealed
R2198	T9418	T9417	pobj	FISH,by
R2199	T9536	T9525	auxpass	are,revealed
R2200	T9537	T9525	advmod	also,revealed
R2201	T9538	T9525	prep	by,revealed
R2202	T9419	T9415	prep	as,revealed
R2203	T9539	T9540	compound	DAPI,staining
R2204	T9540	T9538	pobj	staining,by
R2205	T9541	T9525	prep	as,revealed
R2206	T9420	T9421	amod	large,spots
R2207	T9542	T9543	det	a,signature
R2208	T9543	T9541	pobj	signature,as
R2209	T9544	T9543	amod	typical,signature
R2210	T9421	T9419	pobj	spots,as
R2211	T9545	T9543	prep	of,signature
R2212	T9546	T9545	pobj	p150CAF,of
R2213	T9547	T9546	punct	-,p150CAF
R2214	T9422	T9423	dep	that,coincide
R2215	T9548	T9546	nummod	1,p150CAF
R2216	T9423	T9421	relcl	coincide,spots
R2217	T9549	T9546	appos	loss,p150CAF
R2218	T9550	T9549	punct	-,loss
R2219	T9424	T9423	prep	with,coincide
R2220	T9551	T9549	prep	of,loss
R2221	T9552	T9551	punct	-,of
R2222	T9425	T9426	npadvmod	DAPI,dense
R2223	T9553	T9551	pobj	function,of
R2224	T9554	T9525	punct	.,revealed
R2225	T9556	T9557	nsubj	Quantification,revealed
R2226	T9426	T9428	amod	dense,foci
R2227	T9558	T9556	prep	of,Quantification
R2228	T9559	T9558	pobj	fluorescence,of
R2229	T9427	T9426	punct	-,dense
R2230	T9560	T9556	prep	along,Quantification
R2231	T9561	T9562	det	a,line
R2232	T9428	T9424	pobj	foci,with
R2233	T9562	T9560	pobj	line,along
R2234	T9563	T9564	advmod	randomly,drawn
R2235	T9564	T9562	acl	drawn,line
R2236	T9565	T9564	prep	across,drawn
R2237	T9566	T9567	det	the,nucleus
R2238	T9567	T9565	pobj	nucleus,across
R2239	T9568	T9569	amod	lower,intensity
R2240	T9429	T9423	prep	in,coincide
R2241	T9569	T9557	dobj	intensity,revealed
R2242	T9570	T9569	compound	fluorescence,intensity
R2243	T9571	T9569	cc	and,intensity
R2244	T9430	T9431	det	the,nucleus
R2245	T9572	T9573	det	a,distribution
R2246	T9573	T9569	conj	distribution,intensity
R2247	T9574	T9573	amod	broader,distribution
R2248	T9431	T9429	pobj	nucleus,in
R2249	T9575	T9573	prep	of,distribution
R2250	T9576	T9575	pobj	DAPI,of
R2251	T9577	T9576	cc	and,DAPI
R2252	T9578	T9579	amod	major,signals
R2253	T9432	T9431	compound	interphase,nucleus
R2254	T9579	T9576	conj	signals,DAPI
R2255	T9580	T9579	compound	satellite,signals
R2256	T9433	T9434	punct	(,4A
R2257	T9581	T9579	compound	hybridization,signals
R2258	T9582	T9557	prep	in,revealed
R2259	T9583	T9584	npadvmod	p150CAF,depleted
R2260	T9584	T9588	amod	depleted,cells
R2261	T9434	T9423	parataxis	4A,coincide
R2262	T9585	T9583	punct	-,p150CAF
R2263	T9586	T9583	nummod	1,p150CAF
R2264	T9587	T9584	punct	-,depleted
R2265	T9435	T9434	compound	Figure,4A
R2266	T9588	T9582	pobj	cells,in
R2267	T9589	T9590	punct	(,4C
R2268	T9590	T9557	parataxis	4C,revealed
R2269	T9436	T9434	punct	),4A
R2270	T9591	T9590	compound	Figure,4C
R2271	T9592	T9590	cc	and,4C
R2272	T9593	T9590	conj	4D,4C
R2273	T9437	T9408	punct	.,form
R2274	T9439	T9440	prep	At,form
R2275	T9594	T9590	punct	),4C
R2276	T9441	T9442	det	the,periphery
R2277	T9595	T9557	punct	", ",revealed
R2278	T9596	T9557	advcl	indicating,revealed
R2279	T9597	T9596	dobj	decondensation,indicating
R2280	T9598	T9597	prep	of,decondensation
R2281	T9442	T9439	pobj	periphery,At
R2282	T9599	T9600	amod	pericentric,domains
R2283	T9600	T9598	pobj	domains,of
R2284	T9601	T9600	compound	heterochromatin,domains
R2285	T9602	T9557	punct	.,revealed
R2286	T9604	T9605	prep	In,showed
R2287	T9443	T9442	prep	of,periphery
R2288	T9606	T9604	pobj	contrast,In
R2289	T9607	T9605	punct	", ",showed
R2290	T9608	T9609	amod	centric,domains
R2291	T9444	T9445	det	each,domain
R2292	T9609	T9605	nsubj	domains,showed
R2293	T9610	T9611	det	an,intensity
R2294	T9445	T9443	pobj	domain,of
R2295	T9611	T9605	dobj	intensity,showed
R2296	T9612	T9611	cc	and,intensity
R2297	T9613	T9611	conj	shape,intensity
R2298	T9446	T9445	amod	pericentric,domain
R2299	T9614	T9611	amod	similar,intensity
R2300	T9615	T9614	prep	to,similar
R2301	T9616	T9617	compound	control,cells
R2302	T9447	T9440	punct	", ",form
R2303	T9617	T9615	pobj	cells,to
R2304	T9618	T9605	punct	", ",showed
R2305	T9619	T9605	advcl	suggesting,showed
R2306	T9448	T9449	amod	centric,regions
R2307	T9620	T9621	mark	that,remain
R2308	T9621	T9619	ccomp	remain,suggesting
R2309	T9622	T9621	nsubj	they,remain
R2310	T9449	T9440	nsubj	regions,form
R2311	T9623	T9621	acomp	unaffected,remain
R2312	T9624	T9623	prep	by,unaffected
R2313	T9625	T9626	nmod	p150CAF,depletion
R2314	T9450	T9451	amod	individual,entities
R2315	T9626	T9624	pobj	depletion,by
R2316	T9627	T9625	punct	-,p150CAF
R2317	T9628	T9625	nummod	1,p150CAF
R2318	T9451	T9440	dobj	entities,form
R2319	T9629	T9630	punct	(,4C
R2320	T9630	T9605	parataxis	4C,showed
R2321	T9631	T9630	compound	Figure,4C
R2322	T9452	T9453	punct	(,4A
R2323	T9632	T9630	cc	and,4C
R2324	T9453	T9440	parataxis	4A,form
R2325	T9633	T9630	conj	4D,4C
R2326	T9634	T9630	punct	),4C
R2327	T9635	T9605	punct	.,showed
R2328	T9454	T9453	compound	Figure,4A
R2329	T9637	T9638	prep	In,show
R2330	T9455	T9453	punct	),4A
R2331	T9639	T9637	pobj	conclusion,In
R2332	T9640	T9638	punct	", ",show
R2333	T9641	T9642	det	these,results
R2334	T9642	T9638	nsubj	results,show
R2335	T9456	T9440	punct	.,form
R2336	T9643	T9644	mark	that,required
R2337	T9644	T9638	ccomp	required,show
R2338	T9645	T9644	nsubjpass	p150CAF,required
R2339	T9646	T9645	punct	-,p150CAF
R2340	T9647	T9645	nummod	1,p150CAF
R2341	T9458	T9459	prep	In,observed
R2342	T9648	T9644	auxpass	is,required
R2343	T9649	T9644	prep	for,required
R2344	T9650	T9651	det	the,condensation
R2345	T9651	T9649	pobj	condensation,for
R2346	T9652	T9651	amod	proper,condensation
R2347	T9460	T9461	nmod	p150CAF,cells
R2348	T9653	T9651	cc	and,condensation
R2349	T9654	T9651	conj	clustering,condensation
R2350	T9655	T9651	prep	of,condensation
R2351	T9656	T9657	amod	pericentric,domains
R2352	T9461	T9458	pobj	cells,In
R2353	T9657	T9655	pobj	domains,of
R2354	T9658	T9657	compound	heterochromatin,domains
R2355	T9462	T9460	punct	-,p150CAF
R2356	T9659	T9638	punct	.,show
R2357	T9661	T9662	prep	Given,wondered
R2358	T9463	T9460	nummod	1,p150CAF
R2359	T9663	T9664	det	the,role
R2360	T9664	T9661	pobj	role,Given
R2361	T9464	T9465	npadvmod	RNAi,depleted
R2362	T9665	T9664	amod	known,role
R2363	T9666	T9664	prep	of,role
R2364	T9667	T9666	pobj	CAF,of
R2365	T9465	T9461	amod	depleted,cells
R2366	T9668	T9667	punct	-,CAF
R2367	T9669	T9667	nummod	1,CAF
R2368	T9670	T9664	prep	in,role
R2369	T9466	T9465	punct	-,depleted
R2370	T9671	T9672	det	the,deposition
R2371	T9672	T9670	pobj	deposition,in
R2372	T9673	T9672	prep	of,deposition
R2373	T9674	T9675	compound	histone,H3.1
R2374	T9467	T9459	punct	", ",observed
R2375	T9675	T9673	pobj	H3.1,of
R2376	T9676	T9675	cc	and,H3.1
R2377	T9677	T9675	conj	H4,H3.1
R2378	T9468	T9459	nsubj	we,observed
R2379	T9678	T9675	acl	associated,H3.1
R2380	T9679	T9678	prep	with,associated
R2381	T9680	T9681	compound	DNA,synthesis
R2382	T9469	T9470	det	a,disruption
R2383	T9681	T9679	pobj	synthesis,with
R2384	T9682	T9683	punct	[,12
R2385	T9683	T9664	parataxis	12,role
R2386	T9470	T9459	dobj	disruption,observed
R2387	T9684	T9683	nummod	11,12
R2388	T9685	T9683	punct	",",12
R2389	T9686	T9683	punct	],12
R2390	T9687	T9662	punct	", ",wondered
R2391	T9688	T9662	nsubj	we,wondered
R2392	T9689	T9690	mark	whether,reflect
R2393	T9690	T9662	ccomp	reflect,wondered
R2394	T9691	T9692	det	this,defect
R2395	T9471	T9470	prep	of,disruption
R2396	T9692	T9690	nsubj	defect,reflect
R2397	T9693	T9692	prep	in,defect
R2398	T9694	T9695	amod	higher,order
R2399	T9472	T9473	amod	pericentric,clusters
R2400	T9695	T9697	compound	order,organization
R2401	T9696	T9695	punct	-,order
R2402	T9697	T9693	pobj	organization,in
R2403	T9698	T9697	compound	chromatin,organization
R2404	T9473	T9471	pobj	clusters,of
R2405	T9699	T9690	aux	could,reflect
R2406	T9474	T9473	compound	heterochromatin,clusters
R2407	T9475	T9476	dep	that,coincides
R2408	T9476	T9470	relcl	coincides,disruption
R2409	T9477	T9476	prep	with,coincides
R2410	T9700	T9701	det	an,organization
R2411	T9701	T9690	dobj	organization,reflect
R2412	T9478	T9479	det	the,disappearance
R2413	T9702	T9701	amod	aberrant,organization
R2414	T9703	T9701	amod	nucleosomal,organization
R2415	T9704	T9662	punct	.,wondered
R2416	T9479	T9477	pobj	disappearance,with
R2417	T9706	T9707	advcl	Using,observe
R2418	T9480	T9479	prep	of,disappearance
R2419	T9708	T9706	dobj	DNase,Using
R2420	T9709	T9708	nummod	I,DNase
R2421	T9481	T9482	npadvmod	DAPI,dense
R2422	T9710	T9708	cc	and,DNase
R2423	T9711	T9712	amod	micrococcal,nuclease
R2424	T9712	T9713	nmod	nuclease,assays
R2425	T9482	T9484	amod	dense,foci
R2426	T9713	T9708	conj	assays,DNase
R2427	T9714	T9712	punct	(,nuclease
R2428	T9715	T9712	appos	MNase,nuclease
R2429	T9483	T9482	punct	-,dense
R2430	T9716	T9713	punct	),assays
R2431	T9717	T9707	punct	", ",observe
R2432	T9718	T9707	nsubj	we,observe
R2433	T9484	T9480	pobj	foci,of
R2434	T9719	T9707	aux	could,observe
R2435	T9485	T9486	punct	(,4B
R2436	T9720	T9707	neg	not,observe
R2437	T9486	T9459	parataxis	4B,observed
R2438	T9721	T9722	det	any,difference
R2439	T9722	T9707	dobj	difference,observe
R2440	T9723	T9722	amod	significant,difference
R2441	T9724	T9722	prep	between,difference
R2442	T9725	T9726	nmod	control,cells
R2443	T9487	T9486	compound	Figure,4B
R2444	T9726	T9724	pobj	cells,between
R2445	T9727	T9725	cc	and,control
R2446	T9728	T9729	npadvmod	p150CAF,depleted
R2447	T9729	T9725	conj	depleted,control
R2448	T9806	T9807	det	all,conditions
R2449	T9730	T9728	punct	-,p150CAF
R2450	T9731	T9728	nummod	1,p150CAF
R2451	T9732	T9729	punct	-,depleted
R2452	T9733	T9726	compound	ES,cells
R2453	T9734	T9707	prep	at,observe
R2454	T9735	T9736	det	the,level
R2455	T9807	T9804	pobj	conditions,In
R2456	T9736	T9734	pobj	level,at
R2457	T9737	T9736	prep	of,level
R2458	T9738	T9739	compound	bulk,chromatin
R2459	T9739	T9737	pobj	chromatin,of
R2460	T9808	T9805	punct	", ",was
R2461	T9740	T9739	compound	genome,chromatin
R2462	T9741	T9734	cc	or,at
R2463	T9809	T9810	det	the,amount
R2464	T9742	T9734	conj	at,at
R2465	T9810	T9805	nsubj	amount,was
R2466	T9743	T9744	amod	pericentric,repeats
R2467	T9744	T9742	pobj	repeats,at
R2468	T9811	T9810	prep	of,amount
R2469	T9745	T9746	punct	(,Figure
R2470	T9746	T9707	parataxis	Figure,observe
R2471	T9747	T9746	nummod	5,Figure
R2472	T9748	T9746	punct	),Figure
R2473	T9812	T9813	compound	histone,H3
R2474	T9749	T9707	punct	.,observe
R2475	T9751	T9752	prep	In,compared
R2476	T9813	T9811	pobj	H3,of
R2477	T9753	T9754	det	a,series
R2478	T9814	T9810	acl	remaining,amount
R2479	T9754	T9751	pobj	series,In
R2480	T9755	T9754	amod	second,series
R2481	T9756	T9754	prep	of,series
R2482	T9815	T9810	acl	associated,amount
R2483	T9757	T9756	pobj	experiments,of
R2484	T9758	T9752	punct	", ",compared
R2485	T9759	T9752	nsubj	we,compared
R2486	T9816	T9815	prep	with,associated
R2487	T9760	T9761	det	the,association
R2488	T9761	T9752	dobj	association,compared
R2489	T9762	T9761	prep	of,association
R2490	T9763	T9764	compound	histone,H3
R2491	T9764	T9762	pobj	H3,of
R2492	T9817	T9816	pobj	chromatin,with
R2493	T9765	T9752	prep	with,compared
R2494	T9766	T9765	pobj	chromatin,with
R2495	T9767	T9752	prep	in,compared
R2496	T9768	T9767	pobj	nuclei,in
R2497	T9769	T9768	acl	isolated,nuclei
R2498	T9770	T9769	prep	from,isolated
R2499	T9771	T9770	pobj	cells,from
R2500	T9772	T9771	acl	transfected,cells
R2501	T9818	T9805	acomp	indistinguishable,was
R2502	T9773	T9772	prep	with,transfected
R2503	T9774	T9775	nmod	control,vectors
R2504	T9775	T9773	pobj	vectors,with
R2505	T9776	T9774	cc	and,control
R2506	T9819	T9805	prep	in,was
R2507	T9777	T9774	conj	p150CAF,control
R2508	T9778	T9777	punct	-,p150CAF
R2509	T9779	T9777	nummod	1,p150CAF
R2510	T9820	T9821	nmod	control,cells
R2511	T9780	T9775	compound	RNAi,vectors
R2512	T9781	T9775	compound	plasmid,vectors
R2513	T9782	T9752	punct	.,compared
R2514	T9821	T9819	pobj	cells,in
R2515	T9784	T9785	nsubjpass	Nuclei,incubated
R2516	T9786	T9785	auxpass	were,incubated
R2517	T9822	T9820	cc	and,control
R2518	T9787	T9785	prep	in,incubated
R2519	T9788	T9787	pobj	buffers,in
R2520	T9823	T9824	npadvmod	p150CAF,depleted
R2521	T9789	T9788	prep	with,buffers
R2522	T9790	T9791	amod	different,concentrations
R2523	T9791	T9789	pobj	concentrations,with
R2524	T9824	T9820	conj	depleted,control
R2525	T9792	T9791	compound	salt,concentrations
R2526	T9793	T9791	acl	ranging,concentrations
R2527	T9794	T9793	prep	from,ranging
R2528	T9795	T9796	nummod	100,mM
R2529	T9825	T9823	punct	-,p150CAF
R2530	T9796	T9797	compound	mM,NaCl
R2531	T9797	T9794	pobj	NaCl,from
R2532	T9798	T9794	prep	to,from
R2533	T9826	T9823	nummod	1,p150CAF
R2534	T9799	T9800	nummod	1,M
R2535	T9800	T9801	compound	M,NaCl
R2536	T9801	T9798	pobj	NaCl,to
R2537	T9802	T9785	punct	.,incubated
R2538	T9827	T9824	punct	-,depleted
R2539	T9804	T9805	prep	In,was
R2540	T9828	T9821	compound	ES,cells
R2541	T9829	T9830	punct	(,data
R2542	T9830	T9805	meta	data,was
R2543	T9831	T9830	amod	unpublished,data
R2544	T9912	T9909	parataxis	25,methylation
R2545	T9913	T9912	punct	],25
R2546	T9914	T9906	prep	at,tested
R2547	T9915	T9916	amod	major,repeats
R2548	T9832	T9830	punct	),data
R2549	T9916	T9914	pobj	repeats,at
R2550	T9917	T9916	compound	satellite,repeats
R2551	T9918	T9906	cc	and,tested
R2552	T9919	T9906	conj	found,tested
R2553	T9833	T9805	punct	.,was
R2554	T9920	T9921	det	no,difference
R2555	T9921	T9919	dobj	difference,found
R2556	T9922	T9921	amod	significant,difference
R2557	T9923	T9921	prep	between,difference
R2558	T9835	T9836	advmod	Therefore,are
R2559	T9924	T9925	nmod	control,cells
R2560	T9925	T9923	pobj	cells,between
R2561	T9926	T9924	cc	and,control
R2562	T9927	T9928	npadvmod	p150CAF,depleted
R2563	T9928	T9924	conj	depleted,control
R2564	T9837	T9836	punct	", ",are
R2565	T9929	T9927	punct	-,p150CAF
R2566	T9930	T9927	nummod	1,p150CAF
R2567	T9931	T9928	punct	-,depleted
R2568	T9932	T9933	punct	(,Figure
R2569	T9838	T9839	det	the,loss
R2570	T9933	T9919	parataxis	Figure,found
R2571	T9934	T9933	nummod	6,Figure
R2572	T9935	T9933	punct	),Figure
R2573	T9839	T9836	nsubj	loss,are
R2574	T9936	T9906	punct	.,tested
R2575	T9938	T9939	mark	As,is
R2576	T9840	T9839	prep	of,loss
R2577	T9939	T9952	advcl	is,reduced
R2578	T9940	T9941	det	the,line
R2579	T9941	T9939	nsubj	line,is
R2580	T9841	T9840	pobj	clustering,of
R2581	T9942	T9941	compound	LTM7,line
R2582	T9943	T9941	compound	ES,line
R2583	T9944	T9941	compound	cell,line
R2584	T9842	T9839	cc	and,loss
R2585	T9945	T9946	dep	that,used
R2586	T9946	T9941	relcl	used,line
R2587	T9947	T9946	nsubj	we,used
R2588	T9948	T9946	prep	in,used
R2589	T9843	T9839	conj	decondensation,loss
R2590	T9949	T9950	poss	our,experiments
R2591	T9950	T9948	pobj	experiments,in
R2592	T9951	T9950	compound	RNAi,experiments
R2593	T9844	T9843	prep	of,decondensation
R2594	T9953	T9954	det	a,line
R2595	T9954	T9939	attr	line,is
R2596	T9845	T9846	amod	pericentric,heterochromatin
R2597	T9955	T9954	amod	female,line
R2598	T9956	T9957	punct	(,XX
R2599	T9957	T9955	parataxis	XX,female
R2600	T9958	T9957	punct	),XX
R2601	T9959	T9954	compound	cell,line
R2602	T9960	T9952	punct	", ",reduced
R2603	T9961	T9962	compound	DNA,methylation
R2604	T9962	T9952	nsubjpass	methylation,reduced
R2605	T9846	T9844	pobj	heterochromatin,of
R2606	T9963	T9952	auxpass	is,reduced
R2607	T9964	T9952	advmod	globally,reduced
R2608	T9965	T9952	prep	in,reduced
R2609	T9847	T9836	acomp	unlikely,are
R2610	T9966	T9967	det	these,cells
R2611	T9967	T9965	pobj	cells,in
R2612	T9968	T9969	punct	[,26
R2613	T9969	T9952	parataxis	26,reduced
R2614	T9848	T9849	aux	to,be
R2615	T9970	T9969	punct	],26
R2616	T9971	T9952	punct	.,reduced
R2617	T9849	T9847	xcomp	be,unlikely
R2618	T9973	T9974	aux	To,rule
R2619	T9974	T9975	advcl	rule,tested
R2620	T9976	T9974	prt	out,rule
R2621	T9977	T9978	det	the,possibility
R2622	T9978	T9974	dobj	possibility,rule
R2623	T9979	T9980	mark	that,contribute
R2624	T9850	T9851	det	the,consequence
R2625	T9851	T9849	attr	consequence,be
R2626	T9980	T9978	acl	contribute,possibility
R2627	T9981	T9980	nsubj	hypomethylation,contribute
R2628	T9982	T9981	prep	of,hypomethylation
R2629	T9983	T9984	amod	pericentric,repeats
R2630	T9852	T9851	prep	of,consequence
R2631	T9984	T9982	pobj	repeats,of
R2632	T9985	T9980	aux	might,contribute
R2633	T9986	T9980	prep	to,contribute
R2634	T9987	T9988	det	the,destabilization
R2635	T9853	T9854	amod	severe,defects
R2636	T9988	T9986	pobj	destabilization,to
R2637	T9989	T9988	prep	of,destabilization
R2638	T9990	T9991	compound	heterochromatin,domains
R2639	T9854	T9852	pobj	defects,of
R2640	T9991	T9989	pobj	domains,of
R2641	T9992	T9980	prep	in,contribute
R2642	T9993	T9994	npadvmod	p150CAF,depleted
R2643	T9994	T9998	amod	depleted,cells
R2644	T9995	T9993	punct	-,p150CAF
R2645	T9855	T9854	prep	in,defects
R2646	T9996	T9993	nummod	1,p150CAF
R2647	T9997	T9994	punct	-,depleted
R2648	T9998	T9992	pobj	cells,in
R2649	T9999	T9998	compound	ES,cells
R2650	T10000	T9975	punct	", ",tested
R2651	T10001	T9975	nsubj	we,tested
R2652	T10002	T10003	poss	our,vector
R2653	T10003	T9975	dobj	vector,tested
R2654	T9856	T9857	amod	nucleosomal,organization
R2655	T10004	T10003	compound	RNAi,vector
R2656	T10005	T10003	compound	plasmid,vector
R2657	T10006	T9975	prep	in,tested
R2658	T10007	T10008	det	a,line
R2659	T9857	T9855	pobj	organization,in
R2660	T10008	T10006	pobj	line,in
R2661	T10009	T10008	compound	XY,line
R2662	T10010	T10008	compound	ES,line
R2663	T9858	T9836	punct	.,are
R2664	T10011	T10008	compound	cell,line
R2665	T10012	T9975	punct	", ",tested
R2666	T10013	T9975	cc	and,tested
R2667	T9860	T9861	poss	Our,findings
R2668	T10014	T9975	conj	confirmed,tested
R2669	T10015	T10016	mark	that,leads
R2670	T10016	T10014	ccomp	leads,confirmed
R2671	T9861	T9862	nsubj	findings,showed
R2672	T9863	T9864	mark	that,altered
R2673	T10017	T10016	nsubj	loss,leads
R2674	T10018	T10017	prep	of,loss
R2675	T10019	T10018	pobj	p150CAF,of
R2676	T9864	T9862	ccomp	altered,showed
R2677	T10020	T10019	punct	-,p150CAF
R2678	T10021	T10019	nummod	1,p150CAF
R2679	T10022	T10016	prep	to,leads
R2680	T10023	T10022	pobj	disruption,to
R2681	T9865	T9866	det	the,localization
R2682	T10024	T10023	prep	of,disruption
R2683	T10025	T10026	compound	heterochromatin,organization
R2684	T10026	T10024	pobj	organization,of
R2685	T9866	T9864	nsubjpass	localization,altered
R2686	T10027	T10016	advmod	independently,leads
R2687	T10028	T10027	prep	of,independently
R2688	T10029	T10030	det	the,degree
R2689	T9867	T9866	amod	nuclear,localization
R2690	T10030	T10028	pobj	degree,of
R2691	T10031	T10030	prep	of,degree
R2692	T10032	T10033	compound	DNA,methylation
R2693	T10033	T10031	pobj	methylation,of
R2694	T10034	T10030	prep	at,degree
R2695	T9868	T9866	prep	of,localization
R2696	T10035	T10036	amod	pericentric,repeats
R2697	T10036	T10034	pobj	repeats,at
R2698	T10037	T10038	punct	(,data
R2699	T10038	T10014	meta	data,confirmed
R2700	T10039	T10038	amod	unpublished,data
R2701	T10040	T10038	punct	),data
R2702	T10041	T9975	punct	.,tested
R2703	T10043	T10044	nsubj	We,analyzed
R2704	T9869	T9868	pobj	HP1α,of
R2705	T10045	T10044	advmod	next,analyzed
R2706	T10046	T10047	amod	specific,modifications
R2707	T10047	T10044	dobj	modifications,analyzed
R2708	T10048	T10047	compound	histone,modifications
R2709	T10049	T10050	punct	[,27
R2710	T9870	T9864	auxpass	is,altered
R2711	T10050	T10047	parataxis	27,modifications
R2712	T10051	T10050	punct	],27
R2713	T10052	T10044	prep	in,analyzed
R2714	T9871	T9864	advmod	severely,altered
R2715	T10053	T10054	nmod	chromatin,immunoprecipitation
R2716	T10054	T10055	nmod	immunoprecipitation,experiments
R2717	T10055	T10052	pobj	experiments,in
R2718	T9872	T9864	prep	in,altered
R2719	T10056	T10054	punct	(,immunoprecipitation
R2720	T10057	T10054	appos	ChIP,immunoprecipitation
R2721	T10058	T10055	punct	),experiments
R2722	T10059	T10055	acl	using,experiments
R2723	T9873	T9874	nmod	Chaf1a,embryos
R2724	T10060	T10061	amod	native,chromatin
R2725	T10061	T10059	dobj	chromatin,using
R2726	T9874	T9872	pobj	embryos,in
R2727	T10062	T10063	punct	[,28
R2728	T10063	T10044	parataxis	28,analyzed
R2729	T9875	T9873	punct	−,Chaf1a
R2730	T10064	T10063	punct	],28
R2731	T9876	T9873	punct	/,Chaf1a
R2732	T10065	T10044	punct	.,analyzed
R2733	T10067	T10068	nsubj	We,found
R2734	T9877	T9873	punct	−,Chaf1a
R2735	T10069	T10070	mark	that,immunoprecipitated
R2736	T10070	T10068	ccomp	immunoprecipitated,found
R2737	T9878	T9874	cc	and,embryos
R2738	T10071	T10072	amod	pericentric,DNA
R2739	T10072	T10070	nsubjpass	DNA,immunoprecipitated
R2740	T10073	T10070	auxpass	was,immunoprecipitated
R2741	T10074	T10070	prep	with,immunoprecipitated
R2742	T9879	T9880	npadvmod	p150CAF,depleted
R2743	T10075	T10076	det	a,efficiency
R2744	T10076	T10074	pobj	efficiency,with
R2745	T9880	T9884	amod	depleted,cells
R2746	T10077	T10078	npadvmod	2,lower
R2747	T10078	T10076	amod	lower,efficiency
R2748	T10079	T10077	punct	-,2
R2749	T10080	T10077	advmod	fold,2
R2750	T9881	T9879	punct	-,p150CAF
R2751	T10081	T10070	prep	with,immunoprecipitated
R2752	T10082	T10083	nmod	H4K20me3,antibodies
R2753	T10083	T10081	pobj	antibodies,with
R2754	T10084	T10085	punct	[,30
R2755	T10085	T10082	parataxis	30,H4K20me3
R2756	T10086	T10085	nummod	29,30
R2757	T10087	T10085	punct	",",30
R2758	T9882	T9879	nummod	1,p150CAF
R2759	T10088	T10085	punct	],30
R2760	T10089	T10070	prep	following,immunoprecipitated
R2761	T10090	T10091	nmod	p150CAF,depletion
R2762	T9883	T9880	punct	-,depleted
R2763	T10091	T10089	pobj	depletion,following
R2764	T10092	T10090	punct	-,p150CAF
R2765	T10093	T10090	nummod	1,p150CAF
R2766	T9884	T9874	conj	cells,embryos
R2767	T10094	T10095	punct	(,7A
R2768	T10095	T10068	parataxis	7A,found
R2769	T10096	T10095	compound	Figure,7A
R2770	T10097	T10095	cc	and,7A
R2771	T9885	T9884	compound	ES,cells
R2772	T10098	T10095	conj	7B,7A
R2773	T10099	T10095	punct	),7A
R2774	T10100	T10068	punct	.,found
R2775	T9886	T9862	punct	.,showed
R2776	T10102	T10103	nsubjpass	Immunoprecipitation,affected
R2777	T10104	T10102	prep	of,Immunoprecipitation
R2778	T9888	T9889	nsubj	We,examined
R2779	T10105	T10106	amod	minor,repeats
R2780	T10106	T10104	pobj	repeats,of
R2781	T10107	T10106	compound	satellite,repeats
R2782	T10108	T10102	prep	in,Immunoprecipitation
R2783	T10109	T10110	det	the,experiment
R2784	T9890	T9889	advmod	therefore,examined
R2785	T10110	T10108	pobj	experiment,in
R2786	T10111	T10110	amod	same,experiment
R2787	T10112	T10103	auxpass	was,affected
R2788	T10113	T10103	advmod	less,affected
R2789	T10114	T10115	punct	(,7B
R2790	T9891	T9892	det	the,status
R2791	T10115	T10103	parataxis	7B,affected
R2792	T10116	T10115	compound	Figure,7B
R2793	T10117	T10115	punct	),7B
R2794	T9892	T9889	dobj	status,examined
R2795	T10118	T10103	punct	", ",affected
R2796	T10119	T10103	advcl	showing,affected
R2797	T10120	T10121	mark	that,affects
R2798	T9893	T9892	prep	of,status
R2799	T10121	T10119	ccomp	affects,showing
R2800	T9894	T9895	amod	other,marks
R2801	T9895	T9893	pobj	marks,of
R2802	T9896	T9895	amod	epigenetic,marks
R2803	T10122	T10121	nsubj	loss,affects
R2804	T10123	T10122	prep	of,loss
R2805	T10124	T10125	nmod	p150CAF,function
R2806	T10125	T10123	pobj	function,of
R2807	T10126	T10124	punct	-,p150CAF
R2808	T9897	T9898	advmod	previously,shown
R2809	T10127	T10124	nummod	1,p150CAF
R2810	T10128	T10121	advmod	mainly,affects
R2811	T10129	T10121	dobj	H4K20me3,affects
R2812	T10130	T10129	prep	at,H4K20me3
R2813	T9898	T9895	acl	shown,marks
R2814	T10131	T10132	amod	pericentric,heterochromatin
R2815	T9899	T9900	aux	to,characterize
R2816	T10132	T10130	pobj	heterochromatin,at
R2817	T9900	T9898	xcomp	characterize,shown
R2818	T10133	T10103	punct	.,affected
R2819	T9901	T9902	amod	pericentric,heterochromatin
R2820	T10135	T10136	advmod	Moreover,detected
R2821	T9902	T9900	dobj	heterochromatin,characterize
R2822	T10137	T10136	punct	", ",detected
R2823	T10138	T10139	det	no,difference
R2824	T9903	T9889	punct	.,examined
R2825	T10139	T10136	nsubjpass	difference,detected
R2826	T10140	T10139	amod	significant,difference
R2827	T10141	T10139	prep	in,difference
R2828	T9905	T9906	nsubj	We,tested
R2829	T10142	T10143	compound	immunoprecipitation,efficiency
R2830	T10143	T10141	pobj	efficiency,in
R2831	T10144	T10136	auxpass	was,detected
R2832	T10145	T10136	prep	at,detected
R2833	T9907	T9906	advmod	first,tested
R2834	T10146	T10147	amod	intracisternal,particle
R2835	T10147	T10149	nmod	particle,elements
R2836	T10148	T10147	nmod	A,particle
R2837	T9908	T9909	compound	DNA,methylation
R2838	T10149	T10145	pobj	elements,at
R2839	T10150	T10147	punct	(,particle
R2840	T9909	T9906	dobj	methylation,tested
R2841	T10151	T10147	appos	IAP,particle
R2842	T10152	T10149	punct	),elements
R2843	T10153	T10154	punct	(,7B
R2844	T9910	T9909	compound	CpG,methylation
R2845	T10154	T10149	parataxis	7B,elements
R2846	T10155	T10154	compound	Figure,7B
R2847	T10156	T10154	punct	),7B
R2848	T9911	T9912	punct	[,25
R2849	T10157	T10149	punct	", ",elements
R2850	T10158	T10159	dep	which,are
R2851	T10159	T10149	relcl	are,elements
R2852	T10227	T10224	conj	7B,7A
R2853	T10160	T10161	amod	noncentromeric,elements
R2854	T10161	T10159	attr	elements,are
R2855	T10162	T10161	amod	repeated,elements
R2856	T10163	T10161	compound	DNA,elements
R2857	T10164	T10165	advmod	also,enriched
R2858	T10165	T10161	acl	enriched,elements
R2859	T10166	T10165	prep	in,enriched
R2860	T10167	T10166	pobj	H4K20me3,in
R2861	T10168	T10169	punct	[,31
R2862	T10228	T10224	punct	),7A
R2863	T10169	T10136	parataxis	31,detected
R2864	T10170	T10169	punct	],31
R2865	T10171	T10136	punct	.,detected
R2866	T10229	T10201	punct	.,reduced
R2867	T10173	T10174	det	This,result
R2868	T10174	T10175	nsubj	result,shows
R2869	T10231	T10232	prep	In,are
R2870	T10176	T10177	mark	that,contributes
R2871	T10177	T10175	ccomp	contributes,shows
R2872	T10178	T10177	nsubj	p150CAF,contributes
R2873	T10179	T10178	punct	-,p150CAF
R2874	T10233	T10234	amod	wild,type
R2875	T10180	T10178	nummod	1,p150CAF
R2876	T10181	T10177	prep	to,contributes
R2877	T10182	T10183	amod	normal,levels
R2878	T10183	T10181	pobj	levels,to
R2879	T10234	T10236	compound	type,cells
R2880	T10184	T10183	prep	of,levels
R2881	T10185	T10184	pobj	H4K20me3,of
R2882	T10186	T10177	prep	at,contributes
R2883	T10235	T10234	punct	-,type
R2884	T10187	T10188	amod	pericentric,heterochromatin
R2885	T10188	T10186	pobj	heterochromatin,at
R2886	T10189	T10177	punct	", ",contributes
R2887	T10190	T10177	cc	but,contributes
R2888	T10236	T10231	pobj	cells,In
R2889	T10191	T10192	neg	not,necessarily
R2890	T10192	T10177	conj	necessarily,contributes
R2891	T10237	T10232	punct	", ",are
R2892	T10193	T10192	prep	at,necessarily
R2893	T10194	T10195	amod	other,loci
R2894	T10195	T10193	pobj	loci,at
R2895	T10238	T10232	nsubj	most,are
R2896	T10196	T10175	punct	.,shows
R2897	T10198	T10199	det	The,mark
R2898	T10199	T10201	nsubjpass	mark,reduced
R2899	T10200	T10199	compound	H3K9me3,mark
R2900	T10239	T10238	prep	of,most
R2901	T10202	T10203	punct	[,32
R2902	T10203	T10199	parataxis	32,mark
R2903	T10240	T10241	nmod	H4K20me3,signals
R2904	T10204	T10203	punct	],32
R2905	T10205	T10201	auxpass	was,reduced
R2906	T10206	T10201	advmod	also,reduced
R2907	T10241	T10239	pobj	signals,of
R2908	T10207	T10201	advmod	significantly,reduced
R2909	T10208	T10201	prep	in,reduced
R2910	T10209	T10210	npadvmod	p150CAF,depleted
R2911	T10210	T10214	amod	depleted,cells
R2912	T10211	T10209	punct	-,p150CAF
R2913	T10212	T10209	nummod	1,p150CAF
R2914	T10213	T10210	punct	-,depleted
R2915	T10242	T10240	cc	and,H4K20me3
R2916	T10214	T10208	pobj	cells,in
R2917	T10215	T10201	punct	", ",reduced
R2918	T10216	T10217	mark	though,to
R2919	T10217	T10201	advcl	to,reduced
R2920	T10243	T10240	conj	H3K9me3,H4K20me3
R2921	T10218	T10219	det	a,extent
R2922	T10219	T10217	pobj	extent,to
R2923	T10220	T10219	amod	lesser,extent
R2924	T10244	T10232	acomp	present,are
R2925	T10221	T10219	prep	than,extent
R2926	T10222	T10221	pobj	H4K20me3,than
R2927	T10223	T10224	punct	(,7A
R2928	T10245	T10232	prep	at,are
R2929	T10224	T10201	parataxis	7A,reduced
R2930	T10225	T10224	compound	Figure,7A
R2931	T10226	T10224	cc	and,7A
R2932	T10246	T10247	det	the,level
R2933	T10247	T10245	pobj	level,at
R2934	T10248	T10247	prep	of,level
R2935	T10249	T10250	npadvmod	DAPI,dense
R2937	T10250	T10252	amod	dense,domains
R2939	T10251	T10250	punct	-,dense
R2943	T10252	T10248	pobj	domains,of
R2946	T10253	T10254	advmod	when,visualized
R2950	T10254	T10232	advcl	visualized,are
R2955	T10255	T10254	prep	by,visualized
R2958	T10256	T10255	pobj	IF,by
R2965	T10257	T10258	punct	[,29
R2969	T10258	T10254	parataxis	29,visualized
R2972	T10361	T10362	compound	ES,cells
R2973	T10362	T10360	pobj	cells,in
R2974	T10259	T10258	punct	],29
R2975	T10363	T10354	prep	to,leads
R2976	T10364	T10365	det	a,disruption
R2977	T10365	T10363	pobj	disruption,to
R2978	T10260	T10261	punct	(,7C
R2979	T10366	T10365	amod	simultaneous,disruption
R2980	T10367	T10365	prep	of,disruption
R2981	T10368	T10369	nmod	heterochromatin,organization
R2982	T10261	T10232	parataxis	7C,are
R2983	T10369	T10367	pobj	organization,of
R2984	T10370	T10371	nummod	3,D
R2985	T10371	T10369	compound	D,organization
R2986	T10262	T10261	compound	Figures,7C
R2987	T10372	T10371	punct	-,D
R2988	T10373	T10365	cc	and,disruption
R2989	T10374	T10375	det	an,alteration
R2990	T10375	T10365	conj	alteration,disruption
R2991	T10263	T10261	cc	and,7C
R2992	T10376	T10375	prep	in,alteration
R2993	T10377	T10378	amod	epigenetic,marking
R2994	T10264	T10261	conj	7D,7C
R2995	T10378	T10376	pobj	marking,in
R2996	T10379	T10349	punct	.,show
R2997	T10265	T10261	punct	),7C
R2998	T10266	T10232	punct	.,are
R2999	T10268	T10269	nsubjpass	H3K9me3,perturbed
R3000	T10270	T10269	auxpass	was,perturbed
R3001	T10271	T10269	advmod	severely,perturbed
R3002	T10272	T10269	prep	in,perturbed
R3003	T10273	T10274	npadvmod	p150CAF,depleted
R3004	T10274	T10278	amod	depleted,cells
R3005	T10275	T10273	punct	-,p150CAF
R3006	T10276	T10273	nummod	1,p150CAF
R3007	T10277	T10274	punct	-,depleted
R3008	T10278	T10272	pobj	cells,in
R3009	T10279	T10269	punct	", ",perturbed
R3010	T10280	T10269	advcl	displaying,perturbed
R3011	T10281	T10282	det	a,pattern
R3012	T10282	T10280	dobj	pattern,displaying
R3013	T10283	T10282	amod	diffuse,pattern
R3014	T10284	T10282	amod	similar,pattern
R3015	T10285	T10284	prep	to,similar
R3016	T10286	T10285	pobj	HP1α,to
R3017	T10287	T10288	punct	(,7C
R3018	T10288	T10269	parataxis	7C,perturbed
R3019	T10289	T10288	compound	Figure,7C
R3020	T10290	T10288	punct	),7C
R3021	T10291	T10269	punct	.,perturbed
R3022	T10293	T10294	det	The,pattern
R3023	T10294	T10296	nsubjpass	pattern,altered
R3024	T10295	T10294	compound	H4K20me3,pattern
R3025	T10297	T10296	auxpass	was,altered
R3026	T10298	T10299	advmod	even,more
R3027	T10299	T10300	advmod	more,severely
R3028	T10300	T10296	advmod	severely,altered
R3029	T10301	T10296	punct	", ",altered
R3030	T10302	T10296	advcl	showing,altered
R3031	T10303	T10304	preconj	both,loss
R3032	T10304	T10302	dobj	loss,showing
R3033	T10305	T10304	det	a,loss
R3034	T10306	T10304	prep	of,loss
R3035	T10307	T10308	det	the,dots
R3036	T10308	T10306	pobj	dots,of
R3037	T10309	T10308	amod	typical,dots
R3038	T10310	T10308	acl	revealed,dots
R3039	T10311	T10310	prep	in,revealed
R3040	T10312	T10313	compound	control,cells
R3041	T10313	T10311	pobj	cells,in
R3042	T10314	T10304	cc	and,loss
R3043	T10315	T10316	det	a,reduction
R3044	T10316	T10304	conj	reduction,loss
R3045	T10317	T10316	prep	in,reduction
R3046	T10318	T10319	compound	signal,intensity
R3047	T10319	T10317	pobj	intensity,in
R3048	T10320	T10321	punct	(,7D
R3049	T10321	T10296	parataxis	7D,altered
R3050	T10322	T10321	compound	Figure,7D
R3051	T10323	T10321	punct	),7D
R3052	T10324	T10296	punct	.,altered
R3053	T10326	T10327	advcl	Consistent,detected
R3054	T10328	T10326	prep	with,Consistent
R3055	T10329	T10330	poss	our,experiments
R3056	T10330	T10328	pobj	experiments,with
R3057	T10331	T10330	compound	ChIP,experiments
R3080	T13122	T13123	advmod	Here,provide
R3081	T13124	T13123	punct	", ",provide
R3082	T13125	T13123	nsubj	we,provide
R3083	T13126	T13123	dobj	evidence,provide
R3084	T13127	T13128	mark	that,has
R3085	T13128	T13126	acl	has,evidence
R3086	T13129	T13128	nsubj	p150CAF,has
R3087	T13130	T13129	punct	-,p150CAF
R3088	T13131	T13129	nummod	1,p150CAF
R3089	T13132	T13133	det	a,function
R3090	T13133	T13128	dobj	function,has
R3091	T13134	T13133	amod	crucial,function
R3092	T13135	T13128	prep	during,has
R3093	T13136	T13137	nmod	mouse,embryogenesis
R3094	T13137	T13135	pobj	embryogenesis,during
R3095	T13138	T13137	amod	early,embryogenesis
R3096	T13139	T13135	cc	and,during
R3097	T13140	T13135	conj	in,during
R3098	T13141	T13142	compound	ES,cells
R3099	T13142	T13140	pobj	cells,in
R3100	T13143	T13123	punct	.,provide
R3101	T13145	T13146	amod	Previous,studies
R3102	T13146	T13147	nsubj	studies,showed
R3103	T13148	T13149	mark	that,is
R3104	T13149	T13147	ccomp	is,showed
R3105	T13150	T13149	nsubj	CAF,is
R3106	T13151	T13150	punct	-,CAF
R3107	T13152	T13150	nummod	1,CAF
R3108	T13153	T13149	acomp	essential,is
R3109	T13154	T13153	prep	for,essential
R3110	T13155	T13156	det	the,progression
R3111	T13156	T13154	pobj	progression,for
R3112	T13157	T13156	prep	of,progression
R3113	T13158	T13159	compound	DNA,replication
R3114	T13159	T13157	pobj	replication,of
R3115	T13160	T13149	prep	in,is
R3116	T13161	T13160	pobj	vertebrates,in
R3117	T13162	T13147	punct	.,showed
R3118	T13164	T13165	prep	In,required
R3119	T13166	T13167	compound	Xenopus,laevis
R3120	T13167	T13164	pobj	laevis,In
R3121	T13168	T13165	punct	", ",required
R3122	T13169	T13170	nmod	p150CAF,function
R3123	T13170	T13165	nsubjpass	function,required
R3124	T13171	T13169	punct	-,p150CAF
R3125	T13172	T13169	nummod	1,p150CAF
R3126	T13173	T13165	auxpass	is,required
R3127	T13174	T13165	prep	for,required
R3128	T13175	T13176	det	the,divisions
R3129	T13176	T13174	pobj	divisions,for
R3130	T13177	T13176	amod	rapid,divisions
R3131	T13178	T13176	compound	cell,divisions
R3132	T13179	T13180	dep	that,occur
R3133	T13180	T13176	relcl	occur,divisions
R3134	T13181	T13180	prep	during,occur
R3135	T13182	T13183	amod	early,development
R3136	T13183	T13181	pobj	development,during
R3137	T13184	T13185	punct	[,33
R3138	T13185	T13165	parataxis	33,required
R3139	T13186	T13185	punct	],33
R3140	T13187	T13165	punct	.,required
R3141	T13189	T13190	advmod	Similarly,is
R3142	T13191	T13190	punct	", ",is
R3143	T13192	T13190	nsubj	CAF,is
R3144	T13193	T13192	punct	-,CAF
R3145	T13194	T13192	nummod	1,CAF
R3146	T13195	T13190	acomp	essential,is
R3147	T13196	T13190	prep	in,is
R3148	T13197	T13198	amod	mammalian,lines
R3149	T13198	T13196	pobj	lines,in
R3150	T13199	T13198	compound	cell,lines
R3151	T13200	T13190	prep	for,is
R3152	T13201	T13202	det	the,progression
R3153	T13202	T13200	pobj	progression,for
R3154	T13203	T13202	prep	of,progression
R3155	T13204	T13205	compound	DNA,replication
R3156	T13205	T13203	pobj	replication,of
R3157	T13206	T13207	punct	[,34
R3158	T13207	T13190	parataxis	34,is
R3159	T13208	T13207	nummod	16,34
R3160	T13209	T13207	punct	",",34
R3161	T13210	T13207	nummod	23,34
R3162	T13211	T13207	punct	",",34
R3163	T13212	T13207	nummod	24,34
R3164	T13213	T13207	punct	",",34
R3165	T13214	T13207	punct	],34
R3166	T13215	T13190	punct	.,is
R3167	T13217	T13218	poss	Our,findings
R3168	T13218	T13219	nsubj	findings,reveal
R3169	T13220	T13221	det	a,role
R3170	T13221	T13219	dobj	role,reveal
R3171	T13222	T13221	prep	for,role
R3172	T13223	T13222	pobj	CAF,for
R3173	T13224	T13223	punct	-,CAF
R3174	T13225	T13223	nummod	1,CAF
R3175	T13226	T13221	prep	in,role
R3176	T13227	T13228	det	the,organization
R3177	T13228	T13226	pobj	organization,in
R3178	T13229	T13228	amod	nuclear,organization
R3179	T13230	T13228	prep	of,organization
R3180	T13231	T13232	compound	heterochromatin,domains
R3181	T13232	T13230	pobj	domains,of
R3182	T13233	T13228	prep	during,organization
R3183	T13234	T13235	amod	early,development
R3184	T13235	T13233	pobj	development,during
R3185	T13236	T13233	cc	and,during
R3186	T13237	T13233	conj	in,during
R3187	T13238	T13239	compound	ES,cells
R3188	T13239	T13237	pobj	cells,in
R3189	T13240	T13219	punct	.,reveal
R3190	T13242	T13243	nsubj	We,show
R3191	T13244	T13245	mark	that,observed
R3192	T13245	T13243	ccomp	observed,show
R3193	T13246	T13245	prep	in,observed
R3194	T13247	T13248	npadvmod	p150CAF,depleted
R3195	T13248	T13252	amod	depleted,cells
R3196	T13249	T13247	punct	-,p150CAF
R3197	T13250	T13247	nummod	1,p150CAF
R3198	T13251	T13248	punct	-,depleted
R3199	T13252	T13246	pobj	cells,in
R3200	T13253	T13252	compound	ES,cells
R3201	T13254	T13245	punct	", ",observed
R3202	T13255	T13256	mark	while,persists
R3203	T13256	T13245	advcl	persists,observed
R3204	T13257	T13258	compound	DNA,replication
R3205	T13258	T13256	nsubj	replication,persists
R3206	T13259	T13256	advmod	initially,persists
R3207	T13260	T13256	prep	without,persists
R3208	T13261	T13262	amod	significant,perturbation
R3209	T13262	T13260	pobj	perturbation,without
R3210	T13263	T13262	prep	in,perturbation
R3211	T13264	T13265	amod	nucleosomal,organization
R3212	T13265	T13263	pobj	organization,in
R3213	T13266	T13245	punct	", ",observed
R3214	T13267	T13268	amod	severe,defects
R3215	T13268	T13245	nsubjpass	defects,observed
R3216	T13269	T13268	compound	heterochromatin,defects
R3217	T13270	T13268	compound	organization,defects
R3218	T13271	T13245	aux	can,observed
R3219	T13272	T13245	auxpass	be,observed
R3220	T13273	T13243	punct	.,show
R3221	T13275	T13276	nsubj	Loss,affect
R3222	T13277	T13275	prep	of,Loss
R3223	T13278	T13277	pobj	p150CAF,of
R3224	T13279	T13278	punct	-,p150CAF
R3225	T13280	T13278	nummod	1,p150CAF
R3226	T13281	T13276	aux	did,affect
R3227	T13282	T13276	neg	not,affect
R3228	T13283	T13284	compound	PML,bodies
R3229	T13284	T13276	dobj	bodies,affect
R3230	T13285	T13276	prep	in,affect
R3231	T13286	T13287	compound	ES,nuclei
R3232	T13287	T13285	pobj	nuclei,in
R3233	T13288	T13287	compound	cell,nuclei
R3234	T13289	T13276	punct	", ",affect
R3235	T13290	T13276	advcl	showing,affect
R3236	T13291	T13292	mark	that,altered
R3237	T13292	T13290	ccomp	altered,showing
R3238	T13293	T13294	amod	nuclear,architecture
R3239	T13294	T13292	nsubjpass	architecture,altered
R3240	T13295	T13292	auxpass	is,altered
R3241	T13296	T13292	neg	not,altered
R3242	T13297	T13292	advmod	generally,altered
R3243	T13298	T13276	punct	.,affect
R3244	T13300	T13301	advmod	Ultimately,results
R3245	T13302	T13301	punct	", ",results
R3246	T13303	T13304	nmod	p150CAF,depletion
R3247	T13304	T13301	nsubj	depletion,results
R3248	T13305	T13303	punct	-,p150CAF
R3249	T13306	T13303	nummod	1,p150CAF
R3250	T13307	T13301	prep	in,results
R3251	T13308	T13309	det	the,arrest
R3252	T13309	T13307	pobj	arrest,in
R3253	T13310	T13309	prep	of,arrest
R3254	T13311	T13312	compound	ES,cell
R3255	T13312	T13313	compound	cell,division
R3256	T13313	T13310	pobj	division,of
R3257	T13314	T13309	cc	and,arrest
R3258	T13315	T13316	compound	cell,death
R3259	T13316	T13309	conj	death,arrest
R3260	T13317	T13301	punct	.,results
R3261	T13319	T13320	prep	Given,is
R3262	T13321	T13322	det	the,defects
R3263	T13322	T13319	pobj	defects,Given
R3264	T13323	T13322	amod	major,defects
R3265	T13324	T13322	acl	observed,defects
R3266	T13325	T13324	prep	in,observed
R3267	T13326	T13327	compound	heterochromatin,organization
R3268	T13327	T13325	pobj	organization,in
R3269	T13328	T13320	punct	", ",is
R3270	T13329	T13320	nsubj	it,is
R3271	T13330	T13320	acomp	tempting,is
R3272	T13331	T13332	aux	to,speculate
R3273	T13332	T13320	xcomp	speculate,is
R3274	T13333	T13334	mark	that,cause
R3275	T13334	T13332	ccomp	cause,speculate
R3276	T13335	T13336	det	these,defects
R3277	T13336	T13334	nsubj	defects,cause
R3278	T13337	T13338	det	the,arrest
R3279	T13338	T13334	dobj	arrest,cause
R3280	T13339	T13338	amod	developmental,arrest
R3281	T13340	T13338	prep	in,arrest
R3282	T13341	T13342	det	the,embryos
R3283	T13342	T13340	pobj	embryos,in
R3284	T13343	T13338	cc	and,arrest
R3285	T13344	T13338	conj	death,arrest
R3286	T13345	T13344	prep	in,death
R3287	T13346	T13347	compound	ES,cells
R3288	T13347	T13345	pobj	cells,in
R3289	T13348	T13320	punct	.,is
R3290	T13350	T13351	det	The,role
R3291	T13351	T13352	nsubj	role,includes
R3292	T13353	T13351	prep	of,role
R3293	T13354	T13353	pobj	CAF,of
R3294	T13355	T13354	punct	-,CAF
R3295	T13356	T13354	nummod	1,CAF
R3296	T13357	T13351	prep	in,role
R3297	T13358	T13359	det	the,organization
R3298	T13359	T13357	pobj	organization,in
R3299	T13360	T13359	amod	nuclear,organization
R3300	T13361	T13359	prep	of,organization
R3301	T13362	T13361	pobj	heterochromatin,of
R3302	T13363	T13364	amod	spatial,localization
R3303	T13364	T13352	dobj	localization,includes
R3304	T13365	T13364	punct	", ",localization
R3305	T13366	T13364	conj	condensation,localization
R3306	T13367	T13366	punct	", ",condensation
R3307	T13368	T13366	cc	and,condensation
R3308	T13369	T13366	conj	clustering,condensation
R3309	T13370	T13369	prep	of,clustering
R3310	T13371	T13372	amod	pericentric,domains
R3311	T13372	T13370	pobj	domains,of
R3312	T13373	T13352	punct	.,includes
R3313	T13375	T13376	det	This,function
R3314	T13376	T13378	nsubjpass	function,revealed
R3315	T13377	T13376	amod	important,function
R3316	T13379	T13376	prep	of,function
R3317	T13380	T13379	pobj	CAF,of
R3318	T13381	T13380	punct	-,CAF
R3319	T13382	T13380	nummod	1,CAF
R3320	T13383	T13378	auxpass	was,revealed
R3321	T13384	T13378	neg	not,revealed
R3322	T13385	T13378	prep	in,revealed
R3323	T13386	T13387	amod	primary,MEFs
R3324	T13387	T13385	pobj	MEFs,in
R3325	T13388	T13387	punct	", ",MEFs
R3326	T13389	T13390	compound	3T3,cells
R3327	T13390	T13387	conj	cells,MEFs
R3328	T13391	T13390	punct	", ",cells
R3329	T13392	T13390	cc	or,cells
R3330	T13393	T13394	amod	human,lines
R3331	T13394	T13390	conj	lines,cells
R3332	T13395	T13394	compound	cell,lines
R3333	T13396	T13378	punct	", ",revealed
R3334	T13397	T13378	advcl	suggesting,revealed
R3335	T13398	T13399	mark	that,is
R3336	T13399	T13397	ccomp	is,suggesting
R3337	T13400	T13399	nsubj	it,is
R3338	T13401	T13399	acomp	specific,is
R3339	T13402	T13401	prep	of,specific
R3340	T13403	T13404	amod	pluripotent,cells
R3341	T13404	T13402	pobj	cells,of
R3342	T13405	T13404	amod	embryonic,cells
R3343	T13406	T13378	punct	.,revealed
R3344	T13408	T13409	advmod	Alternatively,represent
R3345	T13410	T13409	punct	", ",represent
R3346	T13411	T13412	amod	early,embryos
R3347	T13412	T13409	nsubj	embryos,represent
R3348	T13413	T13412	cc	and,embryos
R3349	T13414	T13415	compound	ES,cells
R3350	T13415	T13412	conj	cells,embryos
R3351	T13416	T13412	punct	", ",embryos
R3352	T13417	T13418	poss	whose,genome
R3353	T13418	T13419	dep	genome,is
R3354	T13419	T13412	relcl	is,embryos
R3355	T13420	T13421	advmod	more,plastic
R3356	T13421	T13419	acomp	plastic,is
R3357	T13422	T13421	prep	than,plastic
R3358	T13423	T13424	amod	somatic,cells
R3359	T13424	T13422	pobj	cells,than
R3360	T13425	T13409	punct	", ",represent
R3361	T13426	T13409	aux	might,represent
R3362	T13427	T13428	det	a,context
R3363	T13428	T13409	dobj	context,represent
R3364	T13429	T13430	advmod	particularly,sensitive
R3365	T13430	T13428	amod	sensitive,context
R3366	T13431	T13432	prep	in,exacerbated
R3367	T13432	T13428	relcl	exacerbated,context
R3368	T13433	T13431	pobj	which,in
R3369	T13434	T13435	det	the,importance
R3370	T13435	T13432	nsubjpass	importance,exacerbated
R3371	T13436	T13435	prep	of,importance
R3372	T13437	T13436	pobj	CAF,of
R3373	T13438	T13437	punct	-,CAF
R3374	T13439	T13437	nummod	1,CAF
R3375	T13440	T13435	prep	in,importance
R3376	T13441	T13442	compound	heterochromatin,organization
R3377	T13442	T13440	pobj	organization,in
R3378	T13443	T13432	auxpass	is,exacerbated
R3379	T13444	T13409	punct	.,represent
R3380	T13446	T13447	prep	In,are
R3381	T13448	T13449	compound	ES,cells
R3382	T13449	T13446	pobj	cells,In
R3383	T13450	T13447	punct	", ",are
R3384	T13451	T13452	amod	major,proteins
R3385	T13452	T13447	nsubj	proteins,are
R3386	T13453	T13452	amod	architectural,proteins
R3387	T13454	T13452	compound	chromatin,proteins
R3388	T13455	T13447	acomp	hyperdynamic,are
R3389	T13456	T13447	cc	and,are
R3390	T13457	T13447	conj	bind,are
R3391	T13458	T13459	advmod	relatively,loosely
R3392	T13459	T13457	advmod	loosely,bind
R3393	T13460	T13457	prep	to,bind
R3394	T13461	T13460	pobj	chromatin,to
R3395	T13462	T13463	punct	[,35
R3396	T13463	T13457	parataxis	35,bind
R3397	T13464	T13463	punct	],35
R3398	T13465	T13447	punct	.,are
R3399	T13467	T13468	prep	In,show
R3400	T13469	T13470	det	this,context
R3401	T13470	T13467	pobj	context,In
R3402	T13471	T13470	amod	cellular,context
R3403	T13472	T13468	punct	", ",show
R3404	T13473	T13474	poss	our,findings
R3405	T13474	T13468	nsubj	findings,show
R3406	T13475	T13476	mark	that,relies
R3407	T13476	T13468	ccomp	relies,show
R3408	T13477	T13478	amod	proper,architecture
R3409	T13478	T13476	nsubj	architecture,relies
R3410	T13479	T13478	nmod	heterochromatin,architecture
R3411	T13480	T13478	amod	nuclear,architecture
R3412	T13481	T13476	prep	on,relies
R3413	T13482	T13483	nmod	CAF,function
R3414	T13483	T13481	pobj	function,on
R3415	T13484	T13482	punct	-,CAF
R3416	T13485	T13482	nummod	1,CAF
R3417	T13486	T13468	punct	.,show
R3418	T13488	T13489	prep	In,be
R3419	T13490	T13488	pobj	contrast,In
R3420	T13491	T13489	punct	", ",be
R3421	T13492	T13489	prep	in,be
R3422	T13493	T13494	amod	undifferentiated,cells
R3423	T13494	T13492	pobj	cells,in
R3424	T13495	T13496	dep	that,committed
R3425	T13496	T13494	relcl	committed,cells
R3426	T13497	T13496	auxpass	are,committed
R3427	T13498	T13496	advmod	already,committed
R3428	T13499	T13496	dep	lineage,committed
R3429	T13500	T13494	punct	(,cells
R3430	T13501	T13502	amod	such,as
R3431	T13502	T13494	prep	as,cells
R3432	T13503	T13502	pobj	MEFs,as
R3433	T13504	T13489	punct	),be
R3434	T13505	T13506	compound	genome,architecture
R3435	T13506	T13489	nsubj	architecture,be
R3436	T13507	T13489	aux	might,be
R3437	T13508	T13509	advmod	more,stable
R3438	T13509	T13489	acomp	stable,be
R3439	T13510	T13509	prep	than,stable
R3440	T13511	T13510	prep	in,than
R3441	T13512	T13513	compound	ES,cells
R3442	T13513	T13511	pobj	cells,in
R3443	T13514	T13489	cc	and,be
R3444	T13515	T13516	advmod	thus,disrupted
R3445	T13516	T13489	conj	disrupted,be
R3446	T13517	T13516	aux	can,disrupted
R3447	T13518	T13516	neg	not,disrupted
R3448	T13519	T13516	auxpass	be,disrupted
R3449	T13520	T13516	agent	by,disrupted
R3450	T13521	T13522	nmod	CAF,depletion
R3451	T13522	T13520	pobj	depletion,by
R3452	T13523	T13521	punct	-,CAF
R3453	T13524	T13521	nummod	1,CAF
R3454	T13525	T13489	punct	.,be
R3455	T13527	T13528	det	This,function
R3456	T13528	T13530	nsubjpass	function,conserved
R3457	T13529	T13528	amod	important,function
R3458	T13531	T13528	prep	of,function
R3459	T13532	T13531	pobj	CAF,of
R3460	T13533	T13532	punct	-,CAF
R3461	T13534	T13532	nummod	1,CAF
R3462	T13535	T13528	prep	in,function
R3463	T13536	T13537	amod	pluripotent,cells
R3464	T13537	T13535	pobj	cells,in
R3465	T13538	T13537	amod	embryonic,cells
R3466	T13539	T13530	aux	could,conserved
R3467	T13540	T13530	auxpass	be,conserved
R3468	T13541	T13530	advmod	partially,conserved
R3469	T13542	T13530	prep	during,conserved
R3470	T13543	T13542	pobj	evolution,during
R3471	T13544	T13530	punct	.,conserved
R3472	T13546	T13547	advmod	Indeed,associated
R3473	T13548	T13547	punct	", ",associated
R3474	T13549	T13547	prep	in,associated
R3475	T13550	T13551	compound	Arabidopsis,thaliana
R3476	T13551	T13549	pobj	thaliana,in
R3477	T13552	T13551	punct	", ",thaliana
R3478	T13553	T13554	prep	in,is
R3479	T13554	T13551	relcl	is,thaliana
R3480	T13555	T13553	pobj	which,in
R3481	T13556	T13554	nsubj	CAF,is
R3482	T13557	T13556	punct	-,CAF
R3483	T13558	T13556	nummod	1,CAF
R3484	T13559	T13554	neg	not,is
R3485	T13560	T13554	acomp	essential,is
R3486	T13561	T13560	prep	for,essential
R3487	T13562	T13561	pobj	viability,for
R3488	T13563	T13564	punct	[,36
R3489	T13564	T13554	parataxis	36,is
R3490	T13565	T13564	punct	],36
R3491	T13566	T13547	punct	", ",associated
R3492	T13567	T13547	nsubjpass	loss,associated
R3493	T13568	T13567	prep	of,loss
R3494	T13569	T13568	pobj	CAF,of
R3495	T13570	T13569	punct	-,CAF
R3496	T13571	T13569	nummod	1,CAF
R3497	T13572	T13547	auxpass	is,associated
R3498	T13573	T13547	prep	with,associated
R3499	T13574	T13575	det	a,alteration
R3500	T13575	T13573	pobj	alteration,with
R3501	T13576	T13575	amod	mild,alteration
R3502	T13577	T13575	prep	in,alteration
R3503	T13578	T13579	compound	chromocenter,size
R3504	T13579	T13577	pobj	size,in
R3505	T13580	T13581	punct	[,37
R3506	T13581	T13547	parataxis	37,associated
R3507	T13582	T13581	punct	],37
R3508	T13583	T13547	punct	.,associated
R3509	T13585	T13586	det	This,phenotype
R3510	T13586	T13587	nsubj	phenotype,is
R3511	T13588	T13587	advmod	somehow,is
R3512	T13589	T13587	acomp	reminiscent,is
R3513	T13590	T13589	prep	of,reminiscent
R3514	T13591	T13590	pobj	loss,of
R3515	T13592	T13591	prep	of,loss
R3516	T13593	T13594	npadvmod	CAF,mediated
R3517	T13594	T13598	amod	mediated,defects
R3518	T13595	T13593	punct	-,CAF
R3519	T13596	T13593	nummod	1,CAF
R3520	T13597	T13594	punct	-,mediated
R3521	T13598	T13592	pobj	defects,of
R3522	T13599	T13598	prep	in,defects
R3523	T13600	T13601	compound	heterochromatin,organization
R3524	T13601	T13599	pobj	organization,in
R3525	T13602	T13591	prep	in,loss
R3526	T13603	T13604	amod	early,embryos
R3527	T13604	T13602	pobj	embryos,in
R3528	T13605	T13604	compound	mouse,embryos
R3529	T13606	T13587	punct	.,is
R3530	T13608	T13609	prep	In,show
R3531	T13610	T13611	compound	mouse,cells
R3532	T13611	T13608	pobj	cells,In
R3533	T13612	T13611	compound	ES,cells
R3534	T13613	T13609	punct	", ",show
R3535	T13614	T13609	nsubj	we,show
R3536	T13615	T13616	mark	that,required
R3537	T13616	T13609	ccomp	required,show
R3538	T13617	T13616	nsubjpass	CAF,required
R3539	T13618	T13617	punct	-,CAF
R3540	T13619	T13617	nummod	1,CAF
R3541	T13620	T13616	auxpass	is,required
R3542	T13621	T13616	advmod	also,required
R3543	T13622	T13616	prep	for,required
R3544	T13623	T13624	amod	regular,levels
R3545	T13624	T13622	pobj	levels,for
R3546	T13625	T13624	prep	of,levels
R3547	T13626	T13625	pobj	H4K20me3,of
R3548	T13627	T13624	prep	at,levels
R3549	T13628	T13629	amod	pericentric,repeats
R3550	T13629	T13627	pobj	repeats,at
R3551	T13630	T13627	punct	", ",at
R3552	T13631	T13627	cc	but,at
R3553	T13632	T13631	neg	not,but
R3554	T13633	T13627	conj	at,at
R3555	T13634	T13635	compound	IAP,elements
R3556	T13635	T13633	pobj	elements,at
R3557	T13636	T13635	punct	", ",elements
R3558	T13637	T13638	dep	which,are
R3559	T13638	T13635	relcl	are,elements
R3560	T13639	T13640	amod	repeated,sequences
R3561	T13640	T13638	attr	sequences,are
R3562	T13641	T13640	acl	spread,sequences
R3563	T13642	T13643	advmod	all,over
R3564	T13643	T13641	prep	over,spread
R3565	T13644	T13645	det	the,genome
R3566	T13645	T13643	pobj	genome,over
R3567	T13646	T13645	compound	mouse,genome
R3568	T13647	T13609	punct	.,show
R3569	T13649	T13650	det	These,data
R3570	T13650	T13651	nsubj	data,suggest
R3571	T13652	T13653	mark	that,coupled
R3572	T13653	T13651	ccomp	coupled,suggest
R3573	T13654	T13655	amod	proper,organization
R3574	T13655	T13653	nsubjpass	organization,coupled
R3575	T13656	T13655	amod	nuclear,organization
R3576	T13657	T13655	cc	and,organization
R3577	T13658	T13659	amod	epigenetic,marking
R3578	T13659	T13655	conj	marking,organization
R3579	T13660	T13659	prep	of,marking
R3580	T13661	T13662	amod	pericentric,heterochromatin
R3581	T13662	T13660	pobj	heterochromatin,of
R3582	T13663	T13653	auxpass	are,coupled
R3583	T13664	T13653	prep	in,coupled
R3584	T13665	T13666	compound	ES,cells
R3585	T13666	T13664	pobj	cells,in
R3586	T13667	T13651	punct	.,suggest
R3587	T13669	T13670	advmod	Alternatively,represent
R3588	T13671	T13670	punct	", ",represent
R3589	T13672	T13673	nummod	3,D
R3590	T13673	T13675	compound	D,organization
R3591	T13674	T13673	punct	-,D
R3592	T13675	T13670	nsubj	organization,represent
R3593	T13676	T13675	prep	of,organization
R3594	T13677	T13676	pobj	heterochromatin,of
R3595	T13678	T13677	cc	and,heterochromatin
R3596	T13679	T13680	amod	epigenetic,marking
R3597	T13680	T13677	conj	marking,heterochromatin
R3598	T13681	T13670	aux	might,represent
R3599	T13682	T13683	nummod	two,functions
R3600	T13683	T13670	dobj	functions,represent
R3601	T13684	T13683	amod	independent,functions
R3602	T13685	T13683	prep	of,functions
R3603	T13686	T13685	pobj	CAF,of
R3604	T13687	T13686	punct	-,CAF
R3605	T13688	T13686	nummod	1,CAF
R3606	T13689	T13670	punct	.,represent
R3607	T13691	T13692	det	The,requirement
R3608	T13692	T13694	nsubj	requirement,reflect
R3609	T13693	T13692	amod	variable,requirement
R3610	T13695	T13692	prep	for,requirement
R3611	T13696	T13695	pobj	CAF,for
R3612	T13697	T13696	punct	-,CAF
R3613	T13698	T13696	nummod	1,CAF
R3614	T13699	T13692	prep	across,requirement
R3615	T13700	T13699	pobj	species,across
R3616	T13701	T13694	aux	might,reflect
R3617	T13702	T13703	det	the,acquisition
R3618	T13703	T13694	dobj	acquisition,reflect
R3619	T13704	T13703	prep	of,acquisition
R3620	T13705	T13706	amod	new,properties
R3621	T13706	T13704	pobj	properties,of
R3622	T13707	T13706	acl	affecting,properties
R3623	T13708	T13709	det	the,function
R3624	T13709	T13707	dobj	function,affecting
R3625	T13710	T13709	prep	of,function
R3626	T13711	T13712	compound	histone,H3
R3627	T13712	T13713	compound	H3,variants
R3628	T13713	T13710	pobj	variants,of
R3629	T13714	T13703	prep	during,acquisition
R3630	T13715	T13714	pobj	evolution,during
R3631	T13716	T13715	prep	of,evolution
R3632	T13717	T13716	pobj	eukaryotes,of
R3633	T13718	T13694	punct	.,reflect
R3634	T13720	T13721	nsubj	CAF,is
R3635	T13722	T13720	punct	-,CAF
R3636	T13723	T13720	nummod	1,CAF
R3637	T13724	T13721	acomp	essential,is
R3638	T13725	T13721	prep	in,is
R3639	T13726	T13725	pobj	mammals,in
R3640	T13727	T13721	cc	but,is
R3641	T13728	T13721	conj	dispensable,is
R3642	T13729	T13728	prep	in,dispensable
R3643	T13730	T13731	compound	Saccharomyces,cerevisiae
R3644	T13731	T13729	pobj	cerevisiae,in
R3645	T13732	T13733	punct	[,38
R3646	T13733	T13728	parataxis	38,dispensable
R3647	T13734	T13733	punct	],38
R3648	T13735	T13721	punct	.,is
R3649	T13737	T13738	advmod	Interestingly,contain
R3650	T13739	T13738	punct	", ",contain
R3651	T13740	T13741	det	the,genome
R3652	T13741	T13738	nsubj	genome,contain
R3653	T13742	T13741	amod	budding,genome
R3654	T13743	T13741	compound	yeast,genome
R3655	T13744	T13738	aux	does,contain
R3656	T13745	T13738	neg	not,contain
R3657	T13746	T13747	compound	heterochromatin,regions
R3658	T13747	T13738	dobj	regions,contain
R3659	T13748	T13747	amod	equivalent,regions
R3660	T13749	T13748	prep	to,equivalent
R3661	T13750	T13749	pobj	those,to
R3662	T13751	T13750	prep	of,those
R3663	T13752	T13753	amod	higher,eukaryotes
R3664	T13753	T13751	pobj	eukaryotes,of
R3665	T13754	T13738	punct	", ",contain
R3666	T13755	T13738	cc	and,contain
R3667	T13756	T13757	amod	several,features
R3668	T13757	T13759	nsubjpass	features,observed
R3669	T13758	T13757	compound	hallmark,features
R3670	T13759	T13738	conj	observed,contain
R3671	T13760	T13757	prep	of,features
R3672	T13761	T13760	pobj	heterochromatin,of
R3673	T13762	T13763	amod	such,as
R3674	T13763	T13757	prep	as,features
R3675	T13764	T13765	nmod	di,trimethylation
R3676	T13765	T13763	pobj	trimethylation,as
R3677	T13766	T13765	punct	-,trimethylation
R3678	T13767	T13765	cc	and,trimethylation
R3679	T13768	T13765	prep	at,trimethylation
R3680	T13769	T13768	pobj	H3K9,at
R3681	T13770	T13769	punct	", ",H3K9
R3682	T13771	T13769	conj	H3K27,H3K9
R3683	T13772	T13771	punct	", ",H3K27
R3684	T13773	T13771	cc	and,H3K27
R3685	T13774	T13771	conj	H4K20,H3K27
R3686	T13775	T13759	auxpass	are,observed
R3687	T13776	T13759	neg	not,observed
R3688	T13777	T13759	prep	in,observed
R3689	T13778	T13779	det	the,chromatin
R3690	T13779	T13777	pobj	chromatin,in
R3691	T13780	T13779	amod	silent,chromatin
R3692	T13781	T13779	prep	of,chromatin
R3693	T13782	T13783	compound	S.,cerevisiae
R3694	T13783	T13781	pobj	cerevisiae,of
R3695	T13784	T13738	punct	.,contain
R3696	T13786	T13787	prep	In,associated
R3697	T13788	T13786	pobj	mammals,In
R3698	T13789	T13787	punct	", ",associated
R3699	T13790	T13791	compound	H3K9,dimethylation
R3700	T13791	T13787	nsubjpass	dimethylation,associated
R3701	T13792	T13787	auxpass	is,associated
R3702	T13793	T13787	advmod	predominantly,associated
R3703	T13794	T13787	prep	with,associated
R3704	T13795	T13794	pobj	H3.1,with
R3705	T13796	T13794	punct	", ",with
R3706	T13797	T13794	cc	but,with
R3707	T13798	T13797	neg	not,but
R3708	T13799	T13794	conj	with,with
R3709	T13800	T13799	pobj	H3.3,with
R3710	T13801	T13800	punct	", ",H3.3
R3711	T13802	T13803	dep	which,carries
R3712	T13803	T13800	relcl	carries,H3.3
R3713	T13804	T13803	dobj	modifications,carries
R3714	T13805	T13806	advmod	typically,found
R3715	T13806	T13804	acl	found,modifications
R3716	T13807	T13806	prep	in,found
R3717	T13808	T13809	amod	transcribed,regions
R3718	T13809	T13807	pobj	regions,in
R3719	T13810	T13809	compound	chromatin,regions
R3720	T13811	T13812	punct	[,39
R3721	T13812	T13787	parataxis	39,associated
R3722	T13813	T13812	punct	],39
R3723	T13814	T13787	punct	.,associated
R3724	T13816	T13817	prep	Outside,possesses
R3725	T13818	T13816	prep	of,Outside
R3726	T13819	T13820	det	the,variant
R3727	T13820	T13818	pobj	variant,of
R3728	T13821	T13820	amod	centromeric,variant
R3729	T13822	T13820	compound	H3,variant
R3730	T13823	T13817	punct	", ",possesses
R3731	T13824	T13825	compound	S.,cerevisiae
R3732	T13825	T13817	nsubj	cerevisiae,possesses
R3733	T13826	T13827	advmod	only,H3.3
R3734	T13827	T13817	dobj	H3.3,possesses
R3735	T13828	T13827	punct	", ",H3.3
R3736	T13829	T13830	dep	which,expressed
R3737	T13830	T13827	relcl	expressed,H3.3
R3738	T13831	T13830	aux	is,expressed
R3739	T13832	T13830	cc	and,expressed
R3740	T13833	T13830	conj	incorporated,expressed
R3741	T13834	T13833	prep	into,incorporated
R3742	T13835	T13834	pobj	chromatin,into
R3743	T13836	T13830	prep	in,expressed
R3744	T13837	T13838	det	a,fashion
R3745	T13838	T13836	pobj	fashion,in
R3746	T13839	T13840	npadvmod	replication,independent
R3747	T13840	T13838	amod	independent,fashion
R3748	T13841	T13840	punct	-,independent
R3749	T13842	T13830	prep	in,expressed
R3750	T13843	T13844	amod	higher,eukaryotes
R3751	T13844	T13842	pobj	eukaryotes,in
R3752	T13845	T13846	punct	[,12
R3753	T13846	T13817	parataxis	12,possesses
R3754	T13847	T13846	punct	],12
R3755	T13848	T13817	punct	.,possesses
R3756	T13850	T13851	det	These,data
R3757	T13851	T13852	nsubj	data,suggest
R3758	T13853	T13854	det	a,role
R3759	T13854	T13852	dobj	role,suggest
R3760	T13855	T13854	amod	specific,role
R3761	T13856	T13854	prep	for,role
R3762	T13857	T13856	pobj	H3.1,for
R3763	T13858	T13854	prep	in,role
R3764	T13859	T13860	compound	heterochromatin,regions
R3765	T13860	T13858	pobj	regions,in
R3766	T13861	T13852	punct	.,suggest
R3767	T13863	T13864	nsubj	We,show
R3768	T13865	T13864	advmod	here,show
R3769	T13866	T13867	mark	that,required
R3770	T13867	T13864	ccomp	required,show
R3771	T13868	T13867	nsubjpass	CAF,required
R3772	T13869	T13868	punct	-,CAF
R3773	T13870	T13868	nummod	1,CAF
R3774	T13871	T13868	punct	", ",CAF
R3775	T13872	T13873	dep	which,is
R3776	T13873	T13868	relcl	is,CAF
R3777	T13874	T13875	det	a,complex
R3778	T13875	T13873	attr	complex,is
R3779	T13876	T13875	nmod	H3.1,complex
R3780	T13877	T13878	npadvmod	replication,dependent
R3781	T13878	T13875	amod	dependent,complex
R3782	T13879	T13878	punct	-,dependent
R3783	T13880	T13875	compound	loading,complex
R3784	T13881	T13882	punct	[,11
R3785	T13882	T13868	parataxis	11,CAF
R3786	T13883	T13882	punct	],11
R3787	T13884	T13867	punct	", ",required
R3788	T13885	T13867	auxpass	is,required
R3789	T13886	T13867	advmod	specifically,required
R3790	T13887	T13867	prep	for,required
R3791	T13888	T13889	det	the,organization
R3792	T13889	T13887	pobj	organization,for
R3793	T13890	T13889	amod	nuclear,organization
R3794	T13891	T13889	prep	of,organization
R3795	T13892	T13893	amod	pericentric,heterochromatin
R3796	T13893	T13891	pobj	heterochromatin,of
R3797	T13894	T13867	prep	in,required
R3798	T13895	T13894	pobj	embryos,in
R3799	T13896	T13895	cc	and,embryos
R3800	T13897	T13898	compound	ES,cells
R3801	T13898	T13895	conj	cells,embryos
R3802	T13899	T13864	punct	.,show
R3803	T13901	T13902	nummod	One,possibility
R3804	T13902	T13903	nsubj	possibility,be
R3805	T13904	T13903	aux	would,be
R3806	T13905	T13906	det	a,scenario
R3807	T13906	T13903	attr	scenario,be
R3808	T13907	T13908	prep	in,required
R3809	T13908	T13906	relcl	required,scenario
R3810	T13909	T13907	pobj	which,in
R3811	T13910	T13908	nsubjpass	incorporation,required
R3812	T13911	T13910	prep	of,incorporation
R3813	T13912	T13911	pobj	H3.1,of
R3814	T13913	T13910	prep	into,incorporation
R3815	T13914	T13913	pobj	chromatin,into
R3816	T13915	T13908	auxpass	is,required
R3817	T13916	T13908	prep	for,required
R3818	T13917	T13918	det	the,organization
R3819	T13918	T13916	pobj	organization,for
R3820	T13919	T13918	amod	proper,organization
R3821	T13920	T13918	amod	nuclear,organization
R3822	T13921	T13918	prep	of,organization
R3823	T13922	T13923	amod	constitutive,heterochromatin
R3824	T13923	T13921	pobj	heterochromatin,of
R3825	T13924	T13908	prep	in,required
R3826	T13925	T13926	det	an,context
R3827	T13926	T13924	pobj	context,in
R3828	T13927	T13926	amod	embryonic,context
R3829	T13928	T13903	punct	.,be
R3830	T13930	T13931	mark	While,has
R3831	T13931	T13933	advcl	has,is
R3832	T13932	T13931	nsubj	this,has
R3833	T13934	T13935	aux	to,addressed
R3834	T13935	T13931	xcomp	addressed,has
R3835	T13936	T13935	auxpass	be,addressed
R3836	T13937	T13935	advmod	experimentally,addressed
R3837	T13938	T13933	punct	", ",is
R3838	T13939	T13933	nsubj	it,is
R3839	T13940	T13933	acomp	worthwhile,is
R3840	T13941	T13942	aux	to,mention
R3841	T13942	T13933	xcomp	mention,is
R3842	T13943	T13944	det	an,mechanism
R3843	T13944	T13942	dobj	mechanism,mention
R3844	T13945	T13944	amod	alternate,mechanism
R3845	T13946	T13944	amod	possible,mechanism
R3846	T13947	T13948	dep	that,explain
R3847	T13948	T13944	relcl	explain,mechanism
R3848	T13949	T13948	aux	might,explain
R3849	T13950	T13951	det	the,disruption
R3850	T13951	T13948	dobj	disruption,explain
R3851	T13952	T13951	prep	of,disruption
R3852	T13953	T13954	compound	heterochromatin,domains
R3853	T13954	T13952	pobj	domains,of
R3854	T13955	T13951	prep	in,disruption
R3855	T13956	T13957	npadvmod	p150CAF,null
R3856	T13957	T13960	amod	null,embryos
R3857	T13958	T13956	punct	-,p150CAF
R3858	T13959	T13956	nummod	1,p150CAF
R3859	T13960	T13955	pobj	embryos,in
R3860	T13961	T13960	cc	and,embryos
R3861	T13962	T13963	compound	ES,cells
R3862	T13963	T13960	conj	cells,embryos
R3863	T13964	T13933	punct	.,is
R3864	T13966	T13967	nsubjpass	CAF,required
R3865	T13968	T13966	punct	-,CAF
R3866	T13969	T13966	nummod	1,CAF
R3867	T13970	T13967	aux	could,required
R3868	T13971	T13967	auxpass	be,required
R3869	T13972	T13967	prep	for,required
R3870	T13973	T13974	det	the,loading
R3871	T13974	T13972	pobj	loading,for
R3872	T13975	T13974	punct	", ",loading
R3873	T13976	T13974	prep	into,loading
R3874	T13977	T13976	pobj	heterochromatin,into
R3875	T13978	T13974	punct	", ",loading
R3876	T13979	T13974	prep	of,loading
R3877	T13980	T13981	det	an,partner
R3878	T13981	T13979	pobj	partner,of
R3879	T13982	T13981	amod	interacting,partner
R3880	T13983	T13981	acl	required,partner
R3881	T13984	T13983	prep	for,required
R3882	T13985	T13986	det	the,clustering
R3883	T13986	T13984	pobj	clustering,for
R3884	T13987	T13986	prep	of,clustering
R3885	T13988	T13989	amod	pericentric,domains
R3886	T13989	T13987	pobj	domains,of
R3887	T13990	T13967	punct	.,required
R3888	T13992	T13993	prep	Following,become
R3889	T13994	T13995	nummod	one,rounds
R3890	T13995	T13992	pobj	rounds,Following
R3891	T13996	T13994	cc	or,one
R3892	T13997	T13994	conj	two,one
R3893	T13998	T13995	prep	of,rounds
R3894	T13999	T14000	compound	DNA,replication
R3895	T14000	T13998	pobj	replication,of
R3896	T14001	T14000	prep	in,replication
R3897	T14002	T14003	det	the,absence
R3898	T14003	T14001	pobj	absence,in
R3899	T14004	T14003	prep	of,absence
R3900	T14005	T14004	pobj	p150CAF,of
R3901	T14006	T14005	punct	-,p150CAF
R3902	T14007	T14005	nummod	1,p150CAF
R3903	T14008	T13993	punct	", ",become
R3904	T14009	T13993	nsubj	heterochromatin,become
R3905	T14010	T13993	aux	would,become
R3906	T14011	T13993	acomp	deprived,become
R3907	T14012	T14011	prep	of,deprived
R3908	T14013	T14014	det	this,molecule
R3909	T14014	T14012	pobj	molecule,of
R3910	T14015	T13993	punct	", ",become
R3911	T14016	T14017	dep	which,cause
R3912	T14017	T13993	advcl	cause,become
R3913	T14018	T14017	aux	would,cause
R3914	T14019	T14020	det	the,disruption
R3915	T14020	T14017	dobj	disruption,cause
R3916	T14021	T14020	prep	of,disruption
R3917	T14022	T14023	poss	its,organization
R3918	T14023	T14021	pobj	organization,of
R3919	T14024	T14025	nummod	3,D
R3920	T14025	T14023	compound	D,organization
R3921	T14026	T14025	punct	-,D
R3922	T14027	T13993	punct	.,become
R3923	T14029	T14030	det	A,candidate
R3924	T14030	T14032	nsubj	candidate,is
R3925	T14031	T14030	amod	potential,candidate
R3926	T14033	T14034	det	the,protein
R3927	T14034	T14032	attr	protein,is
R3928	T14035	T14034	compound	HP1,protein
R3929	T14036	T14034	punct	", ",protein
R3930	T14037	T14038	dep	which,interacts
R3931	T14038	T14034	relcl	interacts,protein
R3932	T14039	T14038	prep	with,interacts
R3933	T14040	T14039	pobj	CAF,with
R3934	T14041	T14040	punct	-,CAF
R3935	T14042	T14040	nummod	1,CAF
R3936	T14043	T14044	punct	[,16
R3937	T14044	T14032	parataxis	16,is
R3938	T14045	T14044	nummod	13,16
R3939	T14046	T14044	punct	",",16
R3940	T14047	T14044	punct	],16
R3941	T14048	T14032	punct	.,is
R3942	T14050	T14051	prep	In,revealed
R3943	T14052	T14050	pobj	agreement,In
R3944	T14053	T14052	prep	with,agreement
R3945	T14054	T14055	det	a,report
R3946	T14055	T14053	pobj	report,with
R3947	T14056	T14055	amod	recent,report
R3948	T14057	T14058	punct	[,40
R3949	T14058	T14052	parataxis	40,agreement
R3950	T14059	T14058	punct	],40
R3951	T14060	T14051	punct	", ",revealed
R3952	T14061	T14062	poss	our,analysis
R3953	T14062	T14051	nsubj	analysis,revealed
R3954	T14063	T14062	prep	of,analysis
R3955	T14064	T14063	pobj	heterochromatin,of
R3956	T14065	T14064	prep	in,heterochromatin
R3957	T14066	T14067	amod	pre-implantation,embryos
R3958	T14067	T14065	pobj	embryos,in
R3959	T14068	T14051	aux	has,revealed
R3960	T14069	T14070	amod	drastic,changes
R3961	T14070	T14051	dobj	changes,revealed
R3962	T14071	T14070	prep	in,changes
R3963	T14072	T14073	amod	nuclear,organization
R3964	T14073	T14071	pobj	organization,in
R3965	T14074	T14070	prep	between,changes
R3966	T14075	T14076	det	the,stages
R3967	T14076	T14074	pobj	stages,between
R3968	T14077	T14078	nummod	two,cell
R3969	T14078	T14076	nmod	cell,stages
R3970	T14079	T14078	punct	-,cell
R3971	T14080	T14078	cc	and,cell
R3972	T14081	T14078	conj	blastocyst,cell
R3973	T14082	T14051	punct	.,revealed
R3974	T14084	T14085	compound	Cloning,experiments
R3975	T14085	T14086	nsubj	experiments,revealed
R3976	T14087	T14085	prep	in,experiments
R3977	T14088	T14087	pobj	mouse,in
R3978	T14089	T14090	mark	that,reverted
R3979	T14090	T14086	ccomp	reverted,revealed
R3980	T14091	T14092	det	the,organization
R3981	T14092	T14090	nsubjpass	organization,reverted
R3982	T14093	T14092	amod	nuclear,organization
R3983	T14094	T14092	prep	of,organization
R3984	T14095	T14094	pobj	heterochromatin,of
R3985	T14096	T14092	prep	in,organization
R3986	T14097	T14098	compound	ES,nuclei
R3987	T14098	T14096	pobj	nuclei,in
R3988	T14099	T14098	compound	cell,nuclei
R3989	T14100	T14090	auxpass	was,reverted
R3990	T14101	T14090	advmod	quickly,reverted
R3991	T14102	T14090	prep	into,reverted
R3992	T14103	T14104	det	the,form
R3993	T14104	T14102	pobj	form,into
R3994	T14105	T14106	nummod	one,cell
R3995	T14106	T14104	nmod	cell,form
R3996	T14107	T14106	punct	-,cell
R3997	T14108	T14109	npadvmod	stage,specific
R3998	T14109	T14104	amod	specific,form
R3999	T14110	T14109	punct	-,specific
R4000	T14111	T14090	prep	after,reverted
R4001	T14112	T14113	amod	nuclear,transfer
R4002	T14113	T14111	pobj	transfer,after
R4003	T14114	T14115	punct	[,40
R4004	T14115	T14086	parataxis	40,revealed
R4005	T14116	T14115	punct	],40
R4006	T14117	T14086	punct	.,revealed
R4007	T14119	T14120	advmod	Hence,parallels
R4008	T14121	T14120	punct	", ",parallels
R4009	T14122	T14120	nsubj	remodeling,parallels
R4010	T14123	T14122	prep	of,remodeling
R4011	T14124	T14125	nmod	heterochromatin,organization
R4012	T14125	T14123	pobj	organization,of
R4013	T14126	T14127	nummod	3,D
R4014	T14127	T14125	compound	D,organization
R4015	T14128	T14127	punct	-,D
R4016	T14129	T14130	amod	nuclear,reprogramming
R4017	T14130	T14120	dobj	reprogramming,parallels
R4018	T14131	T14130	prep	toward,reprogramming
R4019	T14132	T14133	det	an,status
R4020	T14133	T14131	pobj	status,toward
R4021	T14134	T14135	amod	early,embryonic
R4022	T14135	T14133	amod	embryonic,status
R4023	T14136	T14133	prep	in,status
R4024	T14137	T14138	amod	cloned,embryos
R4025	T14138	T14136	pobj	embryos,in
R4026	T14139	T14120	punct	.,parallels
R4027	T14141	T14142	det	This,reorganization
R4028	T14142	T14144	nsubj	reorganization,is
R4029	T14143	T14142	amod	drastic,reorganization
R4030	T14145	T14142	prep	of,reorganization
R4031	T14146	T14147	compound	heterochromatin,organization
R4032	T14147	T14145	pobj	organization,of
R4033	T14148	T14144	acomp	similar,is
R4034	T14149	T14148	prep	to,similar
R4035	T14150	T14149	pobj	that,to
R4036	T14151	T14150	acl	observed,that
R4037	T14152	T14151	prep	in,observed
R4038	T14153	T14154	npadvmod	p150CAF,depleted
R4039	T14154	T14158	amod	depleted,cells
R4040	T14155	T14153	punct	-,p150CAF
R4041	T14156	T14153	nummod	1,p150CAF
R4042	T14157	T14154	punct	-,depleted
R4043	T14158	T14152	pobj	cells,in
R4044	T14159	T14158	compound	ES,cells
R4045	T14160	T14152	punct	", ",in
R4046	T14161	T14152	cc	and,in
R4047	T14162	T14152	conj	in,in
R4048	T14163	T14164	advmod	developmentally,arrested
R4049	T14164	T14165	amod	arrested,embryos
R4050	T14165	T14162	pobj	embryos,in
R4051	T14166	T14167	nummod	16,cell
R4052	T14167	T14169	nmod	cell,stage
R4053	T14168	T14167	punct	-,cell
R4054	T14169	T14165	nmod	stage,embryos
R4055	T14170	T14165	nmod	Chaf1a,embryos
R4056	T14171	T14170	punct	−,Chaf1a
R4057	T14172	T14170	punct	/,Chaf1a
R4058	T14173	T14170	punct	−,Chaf1a
R4059	T14174	T14144	punct	.,is
R4060	T14176	T14177	det	These,data
R4061	T14177	T14178	nsubj	data,show
R4062	T14179	T14180	mark	that,involved
R4063	T14180	T14178	ccomp	involved,show
R4064	T14181	T14180	nsubjpass	CAF,involved
R4065	T14182	T14181	punct	-,CAF
R4066	T14183	T14181	nummod	1,CAF
R4067	T14184	T14180	auxpass	is,involved
R4068	T14185	T14180	prep	in,involved
R4069	T14186	T14185	pcomp	setting,in
R4070	T14187	T14186	prt	up,setting
R4071	T14188	T14189	amod	proper,architecture
R4072	T14189	T14186	dobj	architecture,setting
R4073	T14190	T14189	compound	heterochromatin,architecture
R4074	T14191	T14186	prep	during,setting
R4075	T14192	T14193	det	the,divisions
R4076	T14193	T14191	pobj	divisions,during
R4077	T14194	T14193	amod	first,divisions
R4078	T14195	T14193	compound	cell,divisions
R4079	T14196	T14193	prep	of,divisions
R4080	T14197	T14198	amod	embryonic,life
R4081	T14198	T14196	pobj	life,of
R4082	T14199	T14178	punct	.,show
R4083	T14201	T14202	prep	Given,suggests
R4084	T14203	T14204	det	the,importance
R4085	T14204	T14201	pobj	importance,Given
R4086	T14205	T14204	prep	of,importance
R4087	T14206	T14207	amod	nuclear,organization
R4088	T14207	T14205	pobj	organization,of
R4089	T14208	T14204	prep	in,importance
R4090	T14209	T14210	det	the,control
R4091	T14210	T14208	pobj	control,in
R4092	T14211	T14210	prep	of,control
R4093	T14212	T14213	compound	gene,expression
R4094	T14213	T14211	pobj	expression,of
R4095	T14214	T14215	punct	[,41
R4096	T14215	T14204	parataxis	41,importance
R4097	T14216	T14215	nummod	21,41
R4098	T14217	T14215	punct	",",41
R4099	T14218	T14215	punct	],41
R4100	T14219	T14202	punct	", ",suggests
R4101	T14220	T14221	det	this,finding
R4102	T14221	T14202	nsubj	finding,suggests
R4103	T14222	T14223	mark	that,contributes
R4104	T14223	T14202	ccomp	contributes,suggests
R4105	T14224	T14223	nsubj	CAF,contributes
R4106	T14225	T14224	punct	-,CAF
R4107	T14226	T14224	nummod	1,CAF
R4108	T14227	T14223	prep	to,contributes
R4109	T14228	T14229	det	the,programs
R4110	T14229	T14227	pobj	programs,to
R4111	T14230	T14229	amod	coordinated,programs
R4112	T14231	T14229	prep	of,programs
R4113	T14232	T14233	compound	gene,expression
R4114	T14233	T14231	pobj	expression,of
R4115	T14234	T14223	prep	during,contributes
R4116	T14235	T14236	amod	early,embryogenesis
R4117	T14236	T14234	pobj	embryogenesis,during
R4118	T14237	T14202	punct	.,suggests
R4119	T14239	T14240	poss	Our,findings
R4120	T14240	T14241	nsubj	findings,open
R4121	T14242	T14243	amod	new,perspectives
R4122	T14243	T14241	dobj	perspectives,open
R4123	T14244	T14243	prep	in,perspectives
R4124	T14245	T14246	det	the,understanding
R4125	T14246	T14244	pobj	understanding,in
R4126	T14247	T14246	prep	of,understanding
R4127	T14248	T14249	compound	chromatin,dynamics
R4128	T14249	T14247	pobj	dynamics,of
R4129	T14250	T14241	prep	during,open
R4130	T14251	T14252	amod	early,development
R4131	T14252	T14250	pobj	development,during
R4132	T14253	T14250	cc	and,during
R4133	T14254	T14250	conj	in,during
R4134	T14255	T14256	amod	pluripotent,cells
R4135	T14256	T14254	pobj	cells,in
R4136	T14257	T14256	amod	embryonic,cells
R4137	T14258	T14241	punct	.,open
R4138	T14260	T14261	mark	While,focused
R4139	T14261	T14264	advcl	focused,point
R4140	T14262	T14263	amod	most,studies
R4141	T14263	T14261	nsubj	studies,focused
R4142	T14265	T14261	prep	on,focused
R4143	T14266	T14267	det	the,role
R4144	T14267	T14265	pobj	role,on
R4145	T14268	T14267	prep	of,role
R4146	T14269	T14270	compound	DNA,methylation
R4147	T14270	T14268	pobj	methylation,of
R4148	T14271	T14270	cc	and,methylation
R4149	T14272	T14273	npadvmod	histone,modifying
R4150	T14273	T14274	amod	modifying,enzymes
R4151	T14274	T14270	conj	enzymes,methylation
R4152	T14275	T14276	punct	[,3
R4153	T14276	T14261	parataxis	3,focused
R4154	T14277	T14276	punct	],3
R4155	T14278	T14264	punct	", ",point
R4156	T14279	T14280	poss	our,data
R4157	T14280	T14264	nsubj	data,point
R4158	T14281	T14264	prep	to,point
R4159	T14282	T14283	det	the,importance
R4160	T14283	T14281	pobj	importance,to
R4161	T14284	T14283	prep	of,importance
R4162	T14285	T14286	det	the,machinery
R4163	T14286	T14284	pobj	machinery,of
R4164	T14287	T14288	compound	chromatin,assembly
R4165	T14288	T14286	compound	assembly,machinery
R4166	T14289	T14264	punct	.,point
R4167	T14291	T14292	nsubj	We,show
R4168	T14293	T14294	mark	that,provides
R4169	T14294	T14292	ccomp	provides,show
R4170	T14295	T14294	prep	in,provides
R4171	T14296	T14295	pobj	addition,in
R4172	T14297	T14296	prep	to,addition
R4173	T14298	T14299	amod	assembling,nucleosomes
R4174	T14299	T14297	pobj	nucleosomes,to
R4175	T14300	T14294	punct	", ",provides
R4176	T14301	T14294	nsubj	CAF,provides
R4177	T14302	T14301	punct	-,CAF
R4178	T14303	T14301	nummod	1,CAF
R4179	T14304	T14305	amod	spatial,information
R4180	T14305	T14294	dobj	information,provides
R4181	T14306	T14304	cc	and,spatial
R4182	T14307	T14304	conj	epigenetic,spatial
R4183	T14308	T14294	prep	to,provides
R4184	T14309	T14310	compound	heterochromatin,domains
R4185	T14310	T14308	pobj	domains,to
R4186	T14311	T14294	prep	in,provides
R4187	T14312	T14313	amod	early,embryos
R4188	T14313	T14311	pobj	embryos,in
R4189	T14314	T14313	cc	and,embryos
R4190	T14315	T14316	compound	ES,cells
R4191	T14316	T14313	conj	cells,embryos
R4192	T14317	T14292	punct	.,show
R4195	T14829	T14828	prep	of,Generation
R4196	T14830	T14831	compound	Chaf1a,mutant
R4197	T14831	T14829	pobj	mutant,of
R4198	T14832	T14831	appos	mice,mutant
R4199	T14833	T14832	cc	and,mice
R4200	T14834	T14832	conj	embryos,mice
R4201	T14835	T14828	punct	.,Generation
R4202	T14837	T14838	advcl	Using,amplified
R4203	T14839	T14837	dobj	PCR,Using
R4204	T14840	T14838	punct	", ",amplified
R4205	T14841	T14838	nsubj	we,amplified
R4206	T14842	T14843	nummod	two,fragments
R4207	T14843	T14838	dobj	fragments,amplified
R4208	T14844	T14843	amod	genomic,fragments
R4209	T14845	T14846	punct	(,kb
R4210	T14846	T14843	parataxis	kb,fragments
R4211	T14847	T14848	quantmod	about,3
R4212	T14848	T14846	nummod	3,kb
R4213	T14849	T14846	appos	each,kb
R4214	T14850	T14846	punct	),kb
R4215	T14851	T14843	acl	flanking,fragments
R4216	T14852	T14853	compound	Chaf1a,exon
R4217	T14853	T14851	dobj	exon,flanking
R4218	T14854	T14853	nummod	3,exon
R4219	T14855	T14838	punct	.,amplified
R4220	T14857	T14858	det	These,fragments
R4221	T14858	T14860	nsubjpass	fragments,assembled
R4222	T14859	T14858	compound	DNA,fragments
R4223	T14861	T14860	auxpass	were,assembled
R4224	T14862	T14860	prep	by,assembled
R4225	T14863	T14864	amod	conventional,cloning
R4226	T14864	T14862	pobj	cloning,by
R4227	T14865	T14864	prep	with,cloning
R4228	T14866	T14867	det	the,cassettes
R4229	T14867	T14865	pobj	cassettes,with
R4230	T14868	T14867	nmod	neomycin,cassettes
R4231	T14869	T14868	cc	and,neomycin
R4232	T14870	T14868	conj	DT,neomycin
R4233	T14871	T14870	punct	(,DT
R4234	T14872	T14873	compound	diphtheria,toxin
R4235	T14873	T14870	appos	toxin,DT
R4236	T14874	T14867	punct	),cassettes
R4237	T14875	T14860	punct	", ",assembled
R4238	T14876	T14877	mark	as,described
R4239	T14877	T14860	advcl	described,assembled
R4240	T14878	T14877	prep	in,described
R4241	T14879	T14880	compound	Figure,1A
R4242	T14880	T14878	pobj	1A,in
R4243	T14881	T14860	punct	.,assembled
R4244	T14883	T14884	det	The,construct
R4245	T14884	T14885	nsubjpass	construct,transfected
R4246	T14886	T14885	auxpass	was,transfected
R4247	T14887	T14885	prep	in,transfected
R4248	T14888	T14889	compound	ES,cells
R4249	T14889	T14887	pobj	cells,in
R4250	T14890	T14885	prep	by,transfected
R4251	T14891	T14890	pobj	electroporation,by
R4252	T14892	T14885	punct	.,transfected
R4253	T14894	T14895	nsubj	We,identified
R4254	T14896	T14897	amod	recombinant,cells
R4255	T14897	T14895	dobj	cells,identified
R4256	T14898	T14897	compound	ES,cells
R4257	T14899	T14897	acl	carrying,cells
R4258	T14900	T14901	det	the,allele
R4259	T14901	T14899	dobj	allele,carrying
R4260	T14902	T14901	nmod	mutant,allele
R4261	T14903	T14901	nmod	Chaf1a tm1Ger,allele
R4262	T14904	T14905	punct	(,abbreviated
R4263	T14905	T14903	parataxis	abbreviated,Chaf1a tm1Ger
R4264	T14906	T14905	oprd	Chaf1a,abbreviated
R4265	T14907	T14906	punct	−,Chaf1a
R4266	T14908	T14905	prep	in,abbreviated
R4267	T14909	T14910	det	the,manuscript
R4268	T14910	T14908	pobj	manuscript,in
R4269	T14911	T14905	punct	),abbreviated
R4270	T14912	T14895	prep	by,identified
R4271	T14913	T14914	compound	Southern,blot
R4272	T14914	T14912	pobj	blot,by
R4273	T14915	T14916	punct	(,1A
R4274	T14916	T14895	parataxis	1A,identified
R4275	T14917	T14916	compound	Figure,1A
R4276	T14918	T14916	punct	),1A
R4277	T14919	T14895	punct	.,identified
R4278	T14921	T14922	nsubj	We,derived
R4279	T14923	T14924	nmod	Chaf1a,mice
R4280	T14924	T14922	dobj	mice,derived
R4281	T14925	T14923	punct	+,Chaf1a
R4282	T14926	T14923	punct	/,Chaf1a
R4283	T14927	T14923	punct	−,Chaf1a
R4284	T14928	T14922	prep	by,derived
R4285	T14929	T14928	pcomp	injecting,by
R4286	T14930	T14931	amod	recombined,cells
R4287	T14931	T14929	dobj	cells,injecting
R4288	T14932	T14931	compound	ES,cells
R4289	T14933	T14929	prep	into,injecting
R4290	T14934	T14935	compound	C57BL,6N
R4291	T14935	T14937	compound	6N,blastocysts
R4292	T14936	T14935	punct	/,6N
R4293	T14937	T14933	pobj	blastocysts,into
R4294	T14938	T14922	punct	.,derived
R4295	T14940	T14941	compound	E4,embryos
R4296	T14941	T14942	nsubjpass	embryos,genotyped
R4297	T14943	T14941	acl	obtained,embryos
R4298	T14944	T14943	prep	from,obtained
R4299	T14945	T14946	det	the,intercross
R4300	T14946	T14944	pobj	intercross,from
R4301	T14947	T14946	prep	of,intercross
R4302	T14948	T14949	nmod	Chaf1a,mice
R4303	T14949	T14947	pobj	mice,of
R4304	T14950	T14948	punct	+,Chaf1a
R4305	T14951	T14948	punct	/,Chaf1a
R4306	T14952	T14948	punct	−,Chaf1a
R4307	T14953	T14942	auxpass	were,genotyped
R4308	T14954	T14942	prep	by,genotyped
R4309	T14955	T14956	compound	nested,PCR
R4310	T14956	T14958	compound	PCR,amplification
R4311	T14957	T14956	punct	-,PCR
R4312	T14958	T14954	pobj	amplification,by
R4313	T14959	T14942	punct	.,genotyped
R4314	T14961	T14962	nsubjpass	Embryos,collected
R4315	T14963	T14962	auxpass	were,collected
R4316	T14964	T14962	prep	in,collected
R4317	T14965	T14966	det	a,mix
R4318	T14966	T14964	pobj	mix,in
R4319	T14967	T14966	compound	PCR,mix
R4320	T14968	T14966	compound	reaction,mix
R4321	T14969	T14966	acl	containing,mix
R4322	T14970	T14971	nmod	oligonucleotide,1
R4323	T14971	T14969	dobj	1,containing
R4324	T14972	T14971	nmod	primers,1
R4325	T14973	T14971	punct	", ",1
R4326	T14974	T14971	conj	2,1
R4327	T14975	T14974	punct	", ",2
R4328	T14976	T14974	cc	and,2
R4329	T14977	T14974	conj	3,2
R4330	T14978	T14979	punct	(,1A
R4331	T14979	T14971	parataxis	1A,1
R4332	T14980	T14979	compound	Figure,1A
R4333	T14981	T14979	punct	),1A
R4334	T14982	T14983	dep	that,amplify
R4335	T14983	T14971	relcl	amplify,1
R4336	T14984	T14985	det	the,alleles
R4337	T14985	T14983	dobj	alleles,amplify
R4338	T14986	T14987	amod	wild,type
R4339	T14987	T14985	nmod	type,alleles
R4340	T14988	T14987	punct	-,type
R4341	T14989	T14987	cc	and,type
R4342	T14990	T14987	conj	mutant,type
R4343	T14991	T14962	punct	.,collected
R4344	T14993	T14994	prep	After,used
R4345	T14995	T14996	nummod	30,cycles
R4346	T14996	T14993	pobj	cycles,After
R4347	T14997	T14996	prep	of,cycles
R4348	T14998	T14997	pobj	amplification,of
R4349	T14999	T14994	punct	", ",used
R4350	T15000	T15001	nummod	1,μl
R4351	T15001	T14994	nsubjpass	μl,used
R4352	T15002	T15001	prep	of,μl
R4353	T15003	T15004	det	each,reaction
R4354	T15004	T15002	pobj	reaction,of
R4355	T15005	T15004	compound	PCR,reaction
R4356	T15006	T14994	auxpass	was,used
R4357	T15007	T14994	prep	in,used
R4358	T15008	T15009	det	a,round
R4359	T15009	T15007	pobj	round,in
R4360	T15010	T15009	amod	second,round
R4361	T15011	T15009	prep	of,round
R4362	T15012	T15013	compound	PCR,amplification
R4363	T15013	T15011	pobj	amplification,of
R4364	T15014	T15009	prep	with,round
R4365	T15015	T15016	det	a,set
R4366	T15016	T15014	pobj	set,with
R4367	T15017	T15016	amod	new,set
R4368	T15018	T15016	prep	of,set
R4369	T15019	T15020	compound	oligonucleotide,primers
R4370	T15020	T15018	pobj	primers,of
R4371	T15021	T14994	punct	.,used
R4372	T15023	T15024	prep	After,run
R4373	T15025	T15026	nummod	30,cycles
R4374	T15026	T15023	pobj	cycles,After
R4375	T15027	T15026	prep	of,cycles
R4376	T15028	T15027	pobj	amplification,of
R4377	T15029	T15024	punct	", ",run
R4378	T15030	T15031	compound	PCR,reactions
R4379	T15031	T15024	nsubjpass	reactions,run
R4380	T15032	T15024	auxpass	were,run
R4381	T15033	T15024	prep	onto,run
R4382	T15034	T15035	det	an,gel
R4383	T15035	T15033	pobj	gel,onto
R4384	T15036	T15035	compound	agarose,gel
R4385	T15037	T15035	punct	", ",gel
R4386	T15038	T15039	dep	which,revealed
R4387	T15039	T15035	relcl	revealed,gel
R4388	T15040	T15041	det	the,presence
R4389	T15041	T15039	dobj	presence,revealed
R4390	T15042	T15041	prep	of,presence
R4391	T15043	T15044	det	the,alleles
R4392	T15044	T15042	pobj	alleles,of
R4393	T15045	T15046	amod	wild,type
R4394	T15046	T15044	nmod	type,alleles
R4395	T15047	T15046	punct	-,type
R4396	T15048	T15049	punct	(,bp
R4397	T15049	T15046	parataxis	bp,type
R4398	T15050	T15049	nummod	150,bp
R4399	T15051	T15049	punct	),bp
R4400	T15052	T15046	cc	and,type
R4401	T15053	T15046	conj	recombinant,type
R4402	T15054	T15055	punct	(,bp
R4403	T15055	T15053	parataxis	bp,recombinant
R4404	T15056	T15055	nummod	200,bp
R4405	T15057	T15055	punct	),bp
R4406	T15058	T15024	punct	.,run
R4407	T15693	T15694	nmod	P150CAF,depletion
R4408	T15695	T15693	punct	-,P150CAF
R4409	T15696	T15693	nummod	1,P150CAF
R4410	T15697	T15694	prep	by,depletion
R4411	T15698	T15697	pobj	RNAi,by
R4412	T15699	T15694	punct	.,depletion
R4413	T15701	T15702	det	The,vector
R4414	T15702	T15705	nsubj	vector,contains
R4415	T15703	T15702	compound	RNAi,vector
R4416	T15704	T15702	compound	plasmid,vector
R4417	T15706	T15707	dep	that,used
R4418	T15707	T15702	relcl	used,vector
R4419	T15708	T15707	nsubj	we,used
R4420	T15709	T15707	prep	in,used
R4421	T15710	T15711	det	this,study
R4422	T15711	T15709	pobj	study,in
R4423	T15712	T15713	det	the,promoter
R4424	T15713	T15705	dobj	promoter,contains
R4425	T15714	T15713	compound	mouse,promoter
R4426	T15715	T15713	compound	H1,promoter
R4427	T15716	T15713	cc	and,promoter
R4428	T15717	T15718	det	a,gene
R4429	T15718	T15713	conj	gene,promoter
R4430	T15719	T15718	compound	puromycin,gene
R4431	T15720	T15718	compound	selection,gene
R4432	T15721	T15722	punct	(,data
R4433	T15722	T15705	parataxis	data,contains
R4434	T15723	T15722	dep	SB,data
R4435	T15724	T15723	cc	and,SB
R4436	T15725	T15723	conj	MG,SB
R4437	T15726	T15722	punct	", ",data
R4438	T15727	T15722	amod	unpublished,data
R4439	T15728	T15722	punct	),data
R4440	T15729	T15705	punct	.,contains
R4441	T15731	T15732	nsubj	We,characterized
R4442	T15732	T15733	ccomp	characterized,is
R4443	T15734	T15732	prep	in,characterized
R4444	T15735	T15734	pobj	detail,in
R4445	T15736	T15737	det	the,properties
R4446	T15737	T15732	dobj	properties,characterized
R4447	T15738	T15737	prep	of,properties
R4448	T15739	T15740	det	this,vector
R4449	T15740	T15738	pobj	vector,of
R4450	T15741	T15740	amod	new,vector
R4451	T15742	T15732	advcl	using,characterized
R4452	T15743	T15744	det	a,gene
R4453	T15744	T15742	dobj	gene,using
R4454	T15745	T15744	compound	GFP,gene
R4455	T15746	T15744	compound	target,gene
R4456	T15747	T15744	cc	and,gene
R4457	T15748	T15744	conj	FACS,gene
R4458	T15749	T15733	punct	: ,is
R4459	T15750	T15733	nsubj	Extinction,is
R4460	T15751	T15750	prep	of,Extinction
R4461	T15752	T15753	compound	target,genes
R4462	T15753	T15751	pobj	genes,of
R4463	T15754	T15750	prep	in,Extinction
R4464	T15755	T15756	compound	ES,cells
R4465	T15756	T15754	pobj	cells,in
R4466	T15757	T15758	advmod	highly,efficient
R4467	T15758	T15733	acomp	efficient,is
R4468	T15759	T15733	prep	in,is
R4469	T15760	T15761	nummod	70,%
R4470	T15761	T15759	pobj	%,in
R4471	T15762	T15761	prep	of,%
R4472	T15763	T15764	det	the,cells
R4473	T15764	T15762	pobj	cells,of
R4474	T15765	T15733	punct	", ",is
R4475	T15766	T15733	conj	of,is
R4476	T15767	T15768	amod	intermediate,efficiency
R4477	T15768	T15766	pobj	efficiency,of
R4478	T15769	T15766	prep	in,of
R4479	T15770	T15771	nummod	15,%
R4480	T15771	T15769	pobj	%,in
R4481	T15772	T15766	punct	", ",of
R4482	T15773	T15766	cc	and,of
R4483	T15774	T15766	conj	inefficient,of
R4484	T15775	T15774	prep	in,inefficient
R4485	T15776	T15777	det	the,%
R4486	T15777	T15775	pobj	%,in
R4487	T15778	T15777	amod	remaining,%
R4488	T15779	T15777	nummod	15,%
R4489	T15780	T15777	prep	of,%
R4490	T15781	T15782	compound	cells,analysis
R4491	T15782	T15780	pobj	analysis,of
R4492	T15783	T15733	punct	.,is
R4493	T15785	T15786	det	The,sequence
R4494	T15786	T15787	nsubjpass	sequence,cloned
R4495	T15788	T15786	prep	of,sequence
R4496	T15789	T15790	nmod	p150CAF,duplex
R4497	T15790	T15788	pobj	duplex,of
R4498	T15791	T15789	punct	-,p150CAF
R4499	T15792	T15789	nummod	1,p150CAF
R4500	T15793	T15790	nmod	siRNA,duplex
R4501	T15794	T15793	punct	(,siRNA
R4502	T15795	T15796	amod	short,RNA
R4503	T15796	T15793	appos	RNA,siRNA
R4504	T15797	T15796	amod	interfering,RNA
R4505	T15798	T15790	punct	),duplex
R4506	T15799	T15800	punct	[,16
R4507	T15800	T15786	parataxis	16,sequence
R4508	T15801	T15800	punct	],16
R4509	T15802	T15787	auxpass	was,cloned
R4510	T15803	T15787	prep	into,cloned
R4511	T15804	T15805	det	this,plasmid
R4512	T15805	T15803	pobj	plasmid,into
R4513	T15806	T15787	punct	.,cloned
R4514	T15808	T15809	det	The,vector
R4515	T15809	T15811	nsubj	vector,expressed
R4516	T15810	T15809	compound	control,vector
R4517	T15812	T15813	det	a,siRNA
R4518	T15813	T15811	dobj	siRNA,expressed
R4519	T15814	T15815	dep	that,targets
R4520	T15815	T15813	relcl	targets,siRNA
R4521	T15816	T15817	compound	GFP,degradation
R4522	T15817	T15815	dobj	degradation,targets
R4523	T15818	T15817	compound	RNA,degradation
R4524	T15819	T15811	punct	.,expressed
R4525	T15821	T15822	compound	ES,cells
R4526	T15822	T15823	nsubjpass	cells,transfected
R4527	T15824	T15823	auxpass	were,transfected
R4528	T15825	T15823	prep	by,transfected
R4529	T15826	T15825	pobj	electroporation,by
R4530	T15827	T15823	prep	with,transfected
R4531	T15828	T15829	det	the,vector
R4532	T15829	T15827	pobj	vector,with
R4533	T15830	T15829	compound	RNA,vector
R4534	T15831	T15829	compound	plasmid,vector
R4535	T15832	T15823	punct	", ",transfected
R4536	T15833	T15823	conj	seeded,transfected
R4537	T15834	T15833	prep	onto,seeded
R4538	T15835	T15836	npadvmod	gelatin,coated
R4539	T15836	T15838	amod	coated,slides
R4540	T15837	T15836	punct	-,coated
R4541	T15838	T15834	pobj	slides,onto
R4542	T15839	T15833	punct	", ",seeded
R4543	T15840	T15833	cc	and,seeded
R4544	T15841	T15833	conj	cultured,seeded
R4545	T15842	T15841	prep	for,cultured
R4546	T15843	T15844	nummod	24,h
R4547	T15844	T15842	pobj	h,for
R4548	T15845	T15841	prep	in,cultured
R4549	T15846	T15847	det	the,absence
R4550	T15847	T15845	pobj	absence,in
R4551	T15848	T15847	prep	of,absence
R4552	T15849	T15848	pobj	selection,of
R4553	T15850	T15823	punct	.,transfected
R4554	T15852	T15853	nsubjpass	Puromycin,added
R4555	T15854	T15855	punct	(,μg
R4556	T15855	T15852	parataxis	μg,Puromycin
R4557	T15856	T15855	nummod	2,μg
R4558	T15857	T15858	punct	/,ml
R4559	T15858	T15855	prep	ml,μg
R4560	T15859	T15855	punct	),μg
R4561	T15860	T15853	auxpass	was,added
R4562	T15861	T15853	prep	to,added
R4563	T15862	T15863	det	the,medium
R4564	T15863	T15861	pobj	medium,to
R4565	T15864	T15863	compound	culture,medium
R4566	T15865	T15853	cc	and,added
R4567	T15866	T15867	nsubjpass	cells,cultured
R4568	T15867	T15853	conj	cultured,added
R4569	T15868	T15867	auxpass	were,cultured
R4570	T15869	T15867	prep	for,cultured
R4571	T15870	T15871	det	an,period
R4572	T15871	T15869	pobj	period,for
R4573	T15872	T15871	amod	additional,period
R4574	T15873	T15874	nummod	48,h
R4575	T15874	T15871	compound	h,period
R4576	T15875	T15874	punct	-,h
R4577	T15876	T15867	punct	.,cultured
R4578	T15878	T15879	advcl	Using,reveals
R4579	T15880	T15881	det	the,vector
R4580	T15881	T15878	dobj	vector,Using
R4581	T15882	T15881	nmod	p150CAF,vector
R4582	T15883	T15882	punct	-,p150CAF
R4583	T15884	T15882	nummod	1,p150CAF
R4584	T15885	T15881	compound	RNAi,vector
R4585	T15886	T15879	punct	", ",reveals
R4586	T15887	T15888	compound	IF,microscopy
R4587	T15888	T15889	compound	microscopy,quantification
R4588	T15889	T15879	nsubj	quantification,reveals
R4589	T15890	T15891	det	a,depletion
R4590	T15891	T15879	dobj	depletion,reveals
R4591	T15892	T15891	amod	complete,depletion
R4592	T15893	T15891	nmod	CAF,depletion
R4593	T15894	T15893	punct	-,CAF
R4594	T15895	T15893	nummod	1,CAF
R4595	T15896	T15879	prep	in,reveals
R4596	T15897	T15898	amod	most,cells
R4597	T15898	T15896	pobj	cells,in
R4598	T15899	T15900	punct	(,S1B
R4599	T15900	T15879	parataxis	S1B,reveals
R4600	T15901	T15900	compound	Figure,S1B
R4601	T15902	T15900	punct	),S1B
R4602	T15903	T15879	punct	.,reveals
R4603	T15905	T15906	compound	Western,blot
R4604	T15906	T15907	compound	blot,analysis
R4605	T15907	T15908	nsubj	analysis,reveals
R4606	T15909	T15910	punct	(,S1A
R4607	T15910	T15907	parataxis	S1A,analysis
R4608	T15911	T15910	compound	Figure,S1A
R4609	T15912	T15910	punct	),S1A
R4610	T15913	T15914	amod	residual,expression
R4611	T15914	T15908	dobj	expression,reveals
R4612	T15915	T15914	nmod	p150CAF,expression
R4613	T15916	T15915	punct	-,p150CAF
R4614	T15917	T15915	nummod	1,p150CAF
R4615	T15918	T15908	punct	", ",reveals
R4616	T15919	T15920	mark	as,expected
R4617	T15920	T15908	advcl	expected,reveals
R4618	T15921	T15920	prep	from,expected
R4619	T15922	T15923	det	the,%
R4620	T15923	T15921	pobj	%,from
R4621	T15924	T15923	nummod	15,%
R4622	T15925	T15923	prep	of,%
R4623	T15926	T15925	pobj	cells,of
R4624	T15927	T15928	dep	that,inhibit
R4625	T15928	T15923	relcl	inhibit,%
R4626	T15929	T15928	aux	do,inhibit
R4627	T15930	T15928	neg	not,inhibit
R4628	T15931	T15932	det	the,gene
R4629	T15932	T15928	dobj	gene,inhibit
R4630	T15933	T15932	compound	target,gene
R4631	T15934	T15908	punct	.,reveals
R4632	T15936	T15937	nsubjpass	MEFs,transfected
R4633	T15938	T15939	punct	(,passage
R4634	T15939	T15936	parataxis	passage,MEFs
R4635	T15940	T15939	nummod	3,passage
R4636	T15941	T15939	punct	", ",passage
R4637	T15942	T15943	nummod	90,%
R4638	T15943	T15944	compound	%,confluence
R4639	T15944	T15939	appos	confluence,passage
R4640	T15945	T15939	punct	),passage
R4641	T15946	T15937	auxpass	were,transfected
R4642	T15947	T15937	prep	during,transfected
R4643	T15948	T15949	nummod	4,h
R4644	T15949	T15947	pobj	h,during
R4645	T15950	T15949	prep	with,h
R4646	T15951	T15952	det	the,vector
R4647	T15952	T15950	pobj	vector,with
R4648	T15953	T15952	compound	RNAi,vector
R4649	T15954	T15952	compound	plasmid,vector
R4650	T15955	T15937	advcl	using,transfected
R4651	T15956	T15955	dobj	Lipofectamine,using
R4652	T15957	T15956	nummod	2000,Lipofectamine
R4653	T15958	T15959	punct	(,Invitrogene
R4654	T15959	T15956	parataxis	Invitrogene,Lipofectamine
R4655	T15960	T15959	punct	", ",Invitrogene
R4656	T15961	T15959	npadvmod	Carlsbad,Invitrogene
R4657	T15962	T15959	punct	", ",Invitrogene
R4658	T15963	T15959	npadvmod	California,Invitrogene
R4659	T15964	T15959	punct	", ",Invitrogene
R4660	T15965	T15966	compound	United,States
R4661	T15966	T15959	npadvmod	States,Invitrogene
R4662	T15967	T15959	punct	),Invitrogene
R4663	T15968	T15937	prep	according,transfected
R4664	T15969	T15968	prep	to,according
R4665	T15970	T15971	poss	manufacturer,conditions
R4666	T15971	T15969	pobj	conditions,to
R4667	T15972	T15970	case	's,manufacturer
R4668	T15973	T15937	punct	.,transfected
R4669	T15975	T15976	nsubjpass	Cells,trypsinized
R4670	T15977	T15976	auxpass	were,trypsinized
R4671	T15978	T15976	punct	", ",trypsinized
R4672	T15979	T15976	conj	plated,trypsinized
R4673	T15980	T15979	prep	at,plated
R4674	T15981	T15982	quantmod	1,3
R4675	T15982	T15984	nummod	3,dilution
R4676	T15983	T15982	punct	/,3
R4677	T15984	T15980	pobj	dilution,at
R4678	T15985	T15979	punct	", ",plated
R4679	T15986	T15979	cc	and,plated
R4680	T15987	T15979	conj	cultured,plated
R4681	T15988	T15987	prep	for,cultured
R4682	T15989	T15990	nummod	48,h
R4683	T15990	T15988	pobj	h,for
R4684	T15991	T15987	prep	in,cultured
R4685	T15992	T15993	det	the,presence
R4686	T15993	T15991	pobj	presence,in
R4687	T15994	T15993	prep	of,presence
R4688	T15995	T15994	pobj	puromycin,of
R4689	T15996	T15976	punct	.,trypsinized
R4690	T16173	T16174	compound	Cell,lines
R4691	T16175	T16174	punct	.,lines
R4692	T16177	T16178	nmod	AT,cells
R4693	T16178	T16182	nsubjpass	cells,used
R4694	T16179	T16177	punct	-,AT
R4695	T16180	T16177	nummod	1,AT
R4696	T16181	T16178	compound	ES,cells
R4697	T16183	T16184	punct	[,42
R4698	T16184	T16178	parataxis	42,cells
R4699	T16185	T16184	punct	],42
R4700	T16186	T16187	punct	(,gift
R4701	T16187	T16178	parataxis	gift,cells
R4702	T16188	T16187	det	a,gift
R4703	T16189	T16187	prep	of,gift
R4704	T16190	T16191	compound	M.,Vernet
R4705	T16191	T16189	pobj	Vernet,of
R4706	T16192	T16187	punct	),gift
R4707	T16193	T16182	auxpass	were,used
R4708	T16194	T16182	prep	for,used
R4709	T16195	T16196	compound	gene,targeting
R4710	T16196	T16194	pobj	targeting,for
R4711	T16197	T16182	punct	.,used
R4712	T16199	T16200	compound	LTM7,cells
R4713	T16200	T16202	nsubjpass	cells,used
R4714	T16201	T16200	compound	ES,cells
R4715	T16203	T16202	auxpass	were,used
R4716	T16204	T16202	prep	in,used
R4717	T16205	T16206	det	all,experiments
R4718	T16206	T16204	pobj	experiments,in
R4719	T16207	T16206	compound	RNAi,experiments
R4720	T16208	T16202	punct	.,used
R4721	T16210	T16211	nsubj	We,derived
R4722	T16212	T16213	det	this,line
R4723	T16213	T16211	dobj	line,derived
R4724	T16214	T16213	compound	cell,line
R4725	T16215	T16211	prep	from,derived
R4726	T16216	T16217	punct	(,C57BL
R4727	T16217	T16218	nmod	C57BL,females
R4728	T16218	T16215	pobj	females,from
R4729	T16219	T16217	punct	/,C57BL
R4730	T16220	T16217	nummod	6,C57BL
R4731	T16221	T16217	punct	×,C57BL
R4732	T16222	T16217	appos	129,C57BL
R4733	T16223	T16217	punct	),C57BL
R4734	T16224	T16218	compound	F1,females
R4735	T16225	T16218	acl	bred,females
R4736	T16226	T16225	prep	with,bred
R4737	T16227	T16228	compound	C3H,HeJ
R4738	T16228	T16230	compound	HeJ,males
R4739	T16229	T16228	punct	/,HeJ
R4740	T16230	T16226	pobj	males,with
R4741	T16231	T16211	punct	.,derived
R4742	T16233	T16234	nsubj	LTM7,are
R4743	T16235	T16236	compound	XX,cells
R4744	T16236	T16234	attr	cells,are
R4745	T16237	T16236	compound	ES,cells
R4746	T16238	T16236	punct	", ",cells
R4747	T16239	T16236	amod	competent,cells
R4748	T16240	T16239	prep	for,competent
R4749	T16241	T16242	compound	germ,line
R4750	T16242	T16243	compound	line,transmission
R4751	T16243	T16240	pobj	transmission,for
R4752	T16244	T16234	punct	.,are
R4753	T16246	T16247	amod	Primary,MEFs
R4754	T16247	T16248	nsubjpass	MEFs,derived
R4755	T16249	T16248	auxpass	were,derived
R4756	T16250	T16248	prep	from,derived
R4757	T16251	T16252	compound	E13,embryos
R4758	T16252	T16250	pobj	embryos,from
R4759	T16253	T16254	mark	as,described
R4760	T16254	T16248	advcl	described,derived
R4761	T16255	T16254	prep	in,described
R4762	T16256	T16255	punct	[,in
R4763	T16257	T16255	pobj	43,in
R4764	T16258	T16248	punct	],derived
R4765	T16259	T16248	punct	.,derived
R4766	T16588	T16587	punct	.,Immunofluorescence
R4767	T16590	T16591	nsubjpass	Cells,fixed
R4768	T16592	T16591	auxpass	were,fixed
R4769	T16593	T16591	prep	for,fixed
R4770	T16594	T16595	nummod	20,min
R4771	T16595	T16593	pobj	min,for
R4772	T16596	T16591	prep	in,fixed
R4773	T16597	T16596	pobj	PBS,in
R4774	T16598	T16591	prep	with,fixed
R4775	T16599	T16600	nummod	4,%
R4776	T16600	T16601	compound	%,paraformaldehyde
R4777	T16601	T16598	pobj	paraformaldehyde,with
R4778	T16602	T16591	punct	", ",fixed
R4779	T16603	T16591	cc	and,fixed
R4780	T16604	T16605	nsubjpass	immunodetection,performed
R4781	T16605	T16591	conj	performed,fixed
R4782	T16606	T16605	auxpass	was,performed
R4783	T16607	T16608	mark	as,described
R4784	T16608	T16605	advcl	described,performed
R4785	T16609	T16608	advmod	previously,described
R4786	T16610	T16611	punct	[,44
R4787	T16611	T16605	parataxis	44,performed
R4788	T16612	T16611	punct	],44
R4789	T16613	T16605	punct	.,performed
R4790	T16615	T16616	compound	E4,embryos
R4791	T16616	T16617	nsubjpass	embryos,collected
R4792	T16618	T16617	auxpass	were,collected
R4793	T16619	T16617	cc	and,collected
R4794	T16620	T16617	conj	treated,collected
R4795	T16621	T16620	prep	with,treated
R4796	T16622	T16623	compound	tyrode,acid
R4797	T16623	T16621	pobj	acid,with
R4798	T16624	T16625	aux	to,remove
R4799	T16625	T16620	advcl	remove,treated
R4800	T16626	T16627	det	the,pellucida
R4801	T16627	T16625	dobj	pellucida,remove
R4802	T16628	T16627	compound	zona,pellucida
R4803	T16629	T16620	punct	", ",treated
R4804	T16630	T16620	conj	deposited,treated
R4805	T16631	T16630	prep	onto,deposited
R4806	T16632	T16633	compound	microscope,slides
R4807	T16633	T16631	pobj	slides,onto
R4808	T16634	T16630	punct	", ",deposited
R4809	T16635	T16630	cc	and,deposited
R4810	T16636	T16630	conj	processed,deposited
R4811	T16637	T16636	prep	for,processed
R4812	T16638	T16637	pobj	immunostaining,for
R4813	T16639	T16640	mark	as,described
R4814	T16640	T16636	advcl	described,processed
R4815	T16641	T16640	prep	for,described
R4816	T16642	T16643	compound	ES,cells
R4817	T16643	T16641	pobj	cells,for
R4818	T16644	T16617	punct	.,collected
R4819	T16646	T16647	nsubjpass	Antibodies,used
R4820	T16648	T16646	appos	anti-HP1α,Antibodies
R4821	T16649	T16650	punct	(,2HP1H5
R4822	T16650	T16648	parataxis	2HP1H5,anti-HP1α
R4823	T16651	T16650	punct	", ",2HP1H5
R4824	T16652	T16650	npadvmod	Euromedex,2HP1H5
R4825	T16653	T16650	punct	", ",2HP1H5
R4826	T16654	T16650	npadvmod	France,2HP1H5
R4827	T16655	T16650	punct	),2HP1H5
R4828	T16656	T16648	punct	", ",anti-HP1α
R4829	T16657	T16648	conj	anti-H4K20me3,anti-HP1α
R4830	T16658	T16659	punct	(,Abcam
R4831	T16659	T16657	parataxis	Abcam,anti-H4K20me3
R4832	T16660	T16659	punct	", ",Abcam
R4833	T16661	T16659	npadvmod	Cambridge,Abcam
R4834	T16662	T16659	punct	", ",Abcam
R4835	T16663	T16664	compound	United,Kingdom
R4836	T16664	T16659	npadvmod	Kingdom,Abcam
R4837	T16665	T16659	punct	),Abcam
R4838	T16666	T16657	punct	", ",anti-H4K20me3
R4839	T16667	T16657	conj	anti-BrdU,anti-H4K20me3
R4840	T16668	T16669	punct	(,DakoCytomation
R4841	T16669	T16667	parataxis	DakoCytomation,anti-BrdU
R4842	T16670	T16669	punct	", ",DakoCytomation
R4843	T16671	T16669	npadvmod	Glostrup,DakoCytomation
R4844	T16672	T16669	punct	", ",DakoCytomation
R4845	T16673	T16669	npadvmod	Denmark,DakoCytomation
R4846	T16674	T16669	punct	),DakoCytomation
R4847	T16675	T16667	punct	", ",anti-BrdU
R4848	T16676	T16667	conj	anti-PCNA,anti-BrdU
R4849	T16677	T16678	punct	(,DakoCytomation
R4850	T16678	T16676	parataxis	DakoCytomation,anti-PCNA
R4851	T16679	T16678	punct	),DakoCytomation
R4852	T16680	T16676	punct	", ",anti-PCNA
R4853	T16681	T16676	cc	and,anti-PCNA
R4854	T16682	T16676	conj	anti-PML,anti-PCNA
R4855	T16683	T16684	punct	(,Biotechnology
R4856	T16684	T16682	parataxis	Biotechnology,anti-PML
R4857	T16685	T16684	compound	Upstate,Biotechnology
R4858	T16686	T16684	punct	", ",Biotechnology
R4859	T16687	T16688	compound	Lake,Placid
R4860	T16688	T16684	npadvmod	Placid,Biotechnology
R4861	T16689	T16684	punct	", ",Biotechnology
R4862	T16690	T16691	compound	New,York
R4863	T16691	T16684	npadvmod	York,Biotechnology
R4864	T16692	T16684	punct	", ",Biotechnology
R4865	T16693	T16694	compound	United,States
R4866	T16694	T16684	npadvmod	States,Biotechnology
R4867	T16695	T16684	punct	),Biotechnology
R4868	T16696	T16647	auxpass	were,used
R4869	T16697	T16647	advmod	all,used
R4870	T16698	T16647	prep	at,used
R4871	T16699	T16700	nummod	1,dilution
R4872	T16700	T16698	pobj	dilution,at
R4873	T16701	T16702	punct	/,1000
R4874	T16702	T16699	prep	1000,1
R4875	T16703	T16647	punct	.,used
R4876	T16705	T16706	nmod	Antibodies,p150CAF1
R4877	T16706	T16708	nsubjpass	p150CAF1,used
R4878	T16707	T16706	amod	anti-mouse,p150CAF1
R4879	T16709	T16710	punct	[,16
R4880	T16710	T16706	parataxis	16,p150CAF1
R4881	T16711	T16710	punct	],16
R4882	T16712	T16706	cc	and,p150CAF1
R4883	T16713	T16706	conj	anti-H3K9me3,p150CAF1
R4884	T16714	T16715	punct	(,Biotechnology
R4885	T16715	T16713	parataxis	Biotechnology,anti-H3K9me3
R4886	T16716	T16715	compound	Upstate,Biotechnology
R4887	T16717	T16715	punct	),Biotechnology
R4888	T16718	T16708	auxpass	were,used
R4889	T16719	T16708	prep	at,used
R4890	T16720	T16719	pobj	1,at
R4891	T16721	T16722	punct	/,250
R4892	T16722	T16720	prep	250,1
R4893	T16723	T16720	cc	and,1
R4894	T16724	T16720	conj	1,1
R4895	T16725	T16726	punct	/,500
R4896	T16726	T16724	prep	500,1
R4897	T16727	T16708	punct	", ",used
R4898	T16728	T16708	advmod	respectively,used
R4899	T16729	T16708	punct	.,used
R4900	T16731	T16732	det	All,antibodies
R4901	T16732	T16734	nsubjpass	antibodies,purchased
R4902	T16733	T16732	amod	secondary,antibodies
R4903	T16735	T16734	auxpass	were,purchased
R4904	T16736	T16734	prep	from,purchased
R4905	T16737	T16738	compound	Molecular,Probes
R4906	T16738	T16736	pobj	Probes,from
R4907	T16739	T16740	punct	(,Sunnyvale
R4908	T16740	T16738	parataxis	Sunnyvale,Probes
R4909	T16741	T16740	punct	", ",Sunnyvale
R4910	T16742	T16740	npadvmod	California,Sunnyvale
R4911	T16743	T16740	punct	", ",Sunnyvale
R4912	T16744	T16745	compound	United,States
R4913	T16745	T16740	npadvmod	States,Sunnyvale
R4914	T16746	T16740	punct	),Sunnyvale
R4915	T16747	T16734	punct	.,purchased
R4916	T16924	T16925	compound	Western,blot
R4917	T16925	T16926	compound	blot,analysis
R4918	T16927	T16926	punct	.,analysis
R4919	T16929	T16930	nsubjpass	Cells,lysed
R4920	T16931	T16930	auxpass	were,lysed
R4921	T16932	T16930	prep	in,lysed
R4922	T16933	T16934	compound	Laemli,buffer
R4923	T16934	T16932	pobj	buffer,in
R4924	T16935	T16930	cc	and,lysed
R4925	T16936	T16930	conj	run,lysed
R4926	T16937	T16936	prep	onto,run
R4927	T16938	T16939	det	a,gel
R4928	T16939	T16937	pobj	gel,onto
R4929	T16940	T16941	nummod	4,%
R4930	T16941	T16939	nmod	%,gel
R4931	T16942	T16943	punct	–,%
R4932	T16943	T16941	prep	%,%
R4933	T16944	T16943	nummod	12,%
R4934	T16945	T16939	compound	SDS,gel
R4935	T16946	T16939	compound	PAGE,gel
R4936	T16947	T16939	compound	gradient,gel
R4937	T16948	T16930	punct	.,lysed
R4938	T16950	T16951	nsubj	We,used
R4939	T16952	T16951	dobj	antibodies,used
R4940	T16953	T16952	prep	against,antibodies
R4941	T16954	T16953	pobj	p150CAF,against
R4942	T16955	T16954	punct	-,p150CAF
R4943	T16956	T16954	nummod	1,p150CAF
R4944	T16957	T16958	punct	(,dilution
R4945	T16958	T16954	parataxis	dilution,p150CAF
R4946	T16959	T16958	punct	[,dilution
R4947	T16960	T16958	dep	16,dilution
R4948	T16961	T16958	punct	],dilution
R4949	T16962	T16958	punct	;,dilution
R4950	T16963	T16958	npadvmod	1,dilution
R4951	T16964	T16965	punct	/,500
R4952	T16965	T16963	prep	500,1
R4953	T16966	T16958	punct	),dilution
R4954	T16967	T16954	punct	", ",p150CAF
R4955	T16968	T16954	conj	HP1α,p150CAF
R4956	T16969	T16970	punct	(,1
R4957	T16970	T16968	parataxis	1,HP1α
R4958	T16971	T16970	dep	2G9,1
R4959	T16972	T16970	punct	", ",1
R4960	T16973	T16970	dep	Euromedex,1
R4961	T16974	T16970	punct	;,1
R4962	T16975	T16976	punct	/,500
R4963	T16976	T16970	prep	500,1
R4964	T16977	T16970	punct	),1
R4965	T16978	T16968	punct	", ",HP1α
R4966	T16979	T16980	compound	Histone,H3
R4967	T16980	T16968	conj	H3,HP1α
R4968	T16981	T16982	punct	(,1
R4969	T16982	T16980	parataxis	1,H3
R4970	T16983	T16982	dep	Abcam,1
R4971	T16984	T16982	punct	;,1
R4972	T16985	T16986	punct	/,500
R4973	T16986	T16982	prep	500,1
R4974	T16987	T16982	punct	),1
R4975	T16988	T16980	punct	", ",H3
R4976	T16989	T16980	conj	H3K9me3,H3
R4977	T16990	T16991	punct	(,1
R4978	T16991	T16989	parataxis	1,H3K9me3
R4979	T16992	T16991	dep	Upstate,1
R4980	T16993	T16991	punct	;,1
R4981	T16994	T16995	punct	/,500
R4982	T16995	T16991	prep	500,1
R4983	T16996	T16991	punct	),1
R4984	T16997	T16989	cc	and,H3K9me3
R4985	T16998	T16999	compound	β,actin
R4986	T16999	T16989	conj	actin,H3K9me3
R4987	T17000	T16999	punct	-,actin
R4988	T17001	T17002	punct	(,1
R4989	T17002	T16999	parataxis	1,actin
R4990	T17003	T17002	dep	Upstate,1
R4991	T17004	T17002	punct	;,1
R4992	T17005	T17006	punct	/,000
R4993	T17006	T17002	prep	000,1
R4994	T17007	T17006	nummod	40,000
R4995	T17008	T17002	punct	),1
R4996	T17009	T16951	punct	.,used
R4997	T17171	T17172	compound	DNA,FISH
R4998	T17173	T17172	punct	.,FISH
R4999	T17175	T17176	nsubjpass	Probes,described
R5000	T17177	T17176	auxpass	were,described
R5001	T17178	T17176	advmod	previously,described
R5002	T17179	T17180	punct	[,20
R5003	T17180	T17176	parataxis	20,described
R5004	T17181	T17180	punct	],20
R5005	T17182	T17176	punct	.,described
R5006	T17184	T17185	nmod	Biotin,dUTP
R5007	T17185	T17189	npadvmod	dUTP,labeled
R5008	T17186	T17185	punct	-,dUTP
R5009	T17187	T17185	nummod	16,dUTP
R5010	T17188	T17185	punct	-,dUTP
R5011	T17189	T17203	amod	labeled,probes
R5012	T17190	T17185	cc	or,dUTP
R5013	T17191	T17192	nmod	Digoxigenin,dUTP
R5014	T17192	T17185	conj	dUTP,dUTP
R5015	T17193	T17192	punct	-,dUTP
R5016	T17194	T17192	nummod	11,dUTP
R5017	T17195	T17192	punct	-,dUTP
R5018	T17196	T17197	punct	(,Roche
R5019	T17197	T17192	parataxis	Roche,dUTP
R5020	T17198	T17197	punct	", ",Roche
R5021	T17199	T17197	npadvmod	Basel,Roche
R5022	T17200	T17197	punct	", ",Roche
R5023	T17201	T17197	npadvmod	Switzerland,Roche
R5024	T17202	T17197	punct	),Roche
R5025	T17203	T17204	nsubjpass	probes,generated
R5026	T17205	T17204	auxpass	were,generated
R5027	T17206	T17204	prep	by,generated
R5028	T17207	T17208	compound	nick,translation
R5029	T17208	T17206	pobj	translation,by
R5030	T17209	T17210	punct	(,Roche
R5031	T17210	T17204	parataxis	Roche,generated
R5032	T17211	T17210	punct	),Roche
R5033	T17212	T17204	cc	and,generated
R5034	T17213	T17214	nsubj	FISH,performed
R5035	T17214	T17204	conj	performed,generated
R5036	T17215	T17216	mark	as,described
R5037	T17216	T17214	advcl	described,performed
R5038	T17217	T17218	punct	[,20
R5039	T17218	T17214	parataxis	20,performed
R5040	T17219	T17218	punct	],20
R5041	T17220	T17214	punct	.,performed
R5042	T17222	T17223	compound	Image,acquisition
R5043	T17223	T17224	nsubjpass	acquisition,performed
R5044	T17225	T17224	auxpass	was,performed
R5045	T17226	T17224	prep	with,performed
R5046	T17227	T17228	det	the,microscope
R5047	T17228	T17226	pobj	microscope,with
R5048	T17229	T17230	compound	Deltavision,RT
R5049	T17230	T17228	compound	RT,microscope
R5050	T17231	T17232	punct	(,objective
R5051	T17232	T17228	parataxis	objective,microscope
R5052	T17233	T17232	dep	100,objective
R5053	T17234	T17233	punct	×,100
R5054	T17235	T17232	punct	", ",objective
R5055	T17236	T17232	nummod	1.4,objective
R5056	T17237	T17232	compound	NA,objective
R5057	T17238	T17232	punct	),objective
R5058	T17239	T17224	punct	", ",performed
R5059	T17240	T17241	nsubjpass	images,deconvoluted
R5060	T17241	T17224	conj	deconvoluted,performed
R5061	T17242	T17241	auxpass	were,deconvoluted
R5062	T17243	T17241	punct	", ",deconvoluted
R5063	T17244	T17241	cc	and,deconvoluted
R5064	T17245	T17246	compound	fluorescence,profiles
R5065	T17246	T17247	nsubj	profiles,measured
R5066	T17247	T17241	conj	measured,deconvoluted
R5067	T17248	T17247	prep	along,measured
R5068	T17249	T17250	det	an,line
R5069	T17250	T17248	pobj	line,along
R5070	T17251	T17250	amod	arbitrary,line
R5071	T17252	T17247	advcl	using,measured
R5072	T17253	T17252	dobj	SoftWorx,using
R5073	T17254	T17247	punct	.,measured
R5074	T17929	T17930	compound	Nuclei,preparation
R5075	T17931	T17930	punct	", ",preparation
R5076	T17932	T17933	compound	nuclease,digestion
R5077	T17933	T17930	conj	digestion,preparation
R5078	T17934	T17933	punct	", ",digestion
R5079	T17935	T17933	cc	and,digestion
R5080	T17936	T17937	amod	biochemical,analysis
R5081	T17937	T17933	conj	analysis,digestion
R5082	T17938	T17937	prep	of,analysis
R5083	T17939	T17938	pobj	chromatin,of
R5084	T17940	T17937	punct	.,analysis
R5085	T17942	T17943	compound	ES,cells
R5086	T17943	T17944	nsubjpass	cells,incubated
R5087	T17945	T17944	auxpass	were,incubated
R5088	T17946	T17944	prep	on,incubated
R5089	T17947	T17946	pobj	ice,on
R5090	T17948	T17944	prep	for,incubated
R5091	T17949	T17950	nummod	10,min
R5092	T17950	T17948	pobj	min,for
R5093	T17951	T17944	prep	in,incubated
R5094	T17952	T17951	pobj	buffer,in
R5095	T17953	T17952	nummod	1,buffer
R5096	T17954	T17955	punct	(,HCl
R5097	T17955	T17952	parataxis	HCl,buffer
R5098	T17956	T17957	nummod	15,mM
R5099	T17957	T17955	compound	mM,HCl
R5100	T17958	T17955	compound	Tris,HCl
R5101	T17959	T17955	punct	-,HCl
R5102	T17960	T17961	punct	[,pH
R5103	T17961	T17955	parataxis	pH,HCl
R5104	T17962	T17961	nummod	7.5,pH
R5105	T17963	T17961	punct	],pH
R5106	T17964	T17955	punct	", ",HCl
R5107	T17965	T17966	nummod	0.3,M
R5108	T17966	T17967	compound	M,sucrose
R5109	T17967	T17955	appos	sucrose,HCl
R5110	T17968	T17955	punct	", ",HCl
R5111	T17969	T17970	nummod	60,mM
R5112	T17970	T17971	compound	mM,KCl
R5113	T17971	T17955	appos	KCl,HCl
R5114	T17972	T17955	punct	", ",HCl
R5115	T17973	T17974	nummod	15,mM
R5116	T17974	T17975	compound	mM,NaCl
R5117	T17975	T17955	appos	NaCl,HCl
R5118	T17976	T17955	punct	", ",HCl
R5119	T17977	T17978	nummod	5,mM
R5120	T17978	T17979	compound	mM,MgCl2
R5121	T17979	T17955	appos	MgCl2,HCl
R5122	T17980	T17955	punct	", ",HCl
R5123	T17981	T17982	nummod	0.1,mM
R5124	T17982	T17983	compound	mM,EGTA
R5125	T17983	T17955	appos	EGTA,HCl
R5126	T17984	T17955	punct	),HCl
R5127	T17985	T17952	prep	with,buffer
R5128	T17986	T17987	nummod	0.15,%
R5129	T17987	T17988	compound	%,IGEPAL
R5130	T17988	T17985	pobj	IGEPAL,with
R5131	T17989	T17990	punct	(,Sigma
R5132	T17990	T17988	parataxis	Sigma,IGEPAL
R5133	T17991	T17990	punct	", ",Sigma
R5134	T17992	T17993	compound	St.,Louis
R5135	T17993	T17990	npadvmod	Louis,Sigma
R5136	T17994	T17990	punct	", ",Sigma
R5137	T17995	T17990	npadvmod	Missouri,Sigma
R5138	T17996	T17990	punct	", ",Sigma
R5139	T17997	T17998	compound	United,States
R5140	T17998	T17990	npadvmod	States,Sigma
R5141	T17999	T17990	punct	),Sigma
R5142	T18000	T17944	punct	.,incubated
R5143	T18002	T18003	nsubjpass	Nuclei,purified
R5144	T18004	T18003	auxpass	were,purified
R5145	T18005	T18003	prep	by,purified
R5146	T18006	T18005	pobj	centrifugation,by
R5147	T18007	T18008	punct	(,g
R5148	T18008	T18006	parataxis	g,centrifugation
R5149	T18009	T18008	nummod	"10,000",g
R5150	T18010	T18009	punct	×,"10,000"
R5151	T18011	T18008	prep	for,g
R5152	T18012	T18013	nummod	30,min
R5153	T18013	T18011	pobj	min,for
R5154	T18014	T18008	punct	", ",g
R5155	T18015	T18008	prep	at,g
R5156	T18016	T18017	nummod	4,°C
R5157	T18017	T18015	pobj	°C,at
R5158	T18018	T18008	punct	),g
R5159	T18019	T18003	prep	on,purified
R5160	T18020	T18021	compound	sucrose,cushions
R5161	T18021	T18019	pobj	cushions,on
R5162	T18022	T18023	punct	(,buffer
R5163	T18023	T18021	parataxis	buffer,cushions
R5164	T18024	T18023	nummod	1,buffer
R5165	T18025	T18023	prep	with,buffer
R5166	T18026	T18027	nummod	1.2,M
R5167	T18027	T18028	compound	M,sucrose
R5168	T18028	T18025	pobj	sucrose,with
R5169	T18029	T18023	punct	),buffer
R5170	T18030	T18003	cc	and,purified
R5171	T18031	T18003	conj	resuspended,purified
R5172	T18032	T18031	prep	in,resuspended
R5173	T18033	T18034	compound	nuclease,buffer
R5174	T18034	T18032	pobj	buffer,in
R5175	T18035	T18036	punct	(,HCl
R5176	T18036	T18034	parataxis	HCl,buffer
R5177	T18037	T18038	nummod	50,mM
R5178	T18038	T18036	compound	mM,HCl
R5179	T18039	T18036	compound	Tris,HCl
R5180	T18040	T18036	punct	-,HCl
R5181	T18041	T18042	punct	[,pH
R5182	T18042	T18036	parataxis	pH,HCl
R5183	T18043	T18042	nummod	7.5,pH
R5184	T18044	T18042	punct	],pH
R5185	T18045	T18036	punct	", ",HCl
R5186	T18046	T18047	nummod	20,mM
R5187	T18047	T18048	compound	mM,NaCl
R5188	T18048	T18036	appos	NaCl,HCl
R5189	T18049	T18036	punct	", ",HCl
R5190	T18050	T18051	nummod	0.32,M
R5191	T18051	T18052	compound	M,sucrose
R5192	T18052	T18036	appos	sucrose,HCl
R5193	T18053	T18036	punct	", ",HCl
R5194	T18054	T18055	nummod	4,mM
R5195	T18055	T18056	compound	mM,MgCl2
R5196	T18056	T18036	appos	MgCl2,HCl
R5197	T18057	T18036	punct	", ",HCl
R5198	T18058	T18059	nummod	1,mM
R5199	T18059	T18060	compound	mM,CaCl2
R5200	T18060	T18036	appos	CaCl2,HCl
R5201	T18061	T18036	punct	),HCl
R5202	T18062	T18003	punct	.,purified
R5203	T18064	T18065	quantmod	About,2.106
R5204	T18065	T18066	nummod	2.106,nuclei
R5205	T18066	T18067	nsubjpass	nuclei,incubated
R5206	T18068	T18067	auxpass	were,incubated
R5207	T18069	T18067	prep	with,incubated
R5208	T18070	T18071	amod	increasing,quantities
R5209	T18071	T18069	pobj	quantities,with
R5210	T18072	T18071	prep	of,quantities
R5211	T18073	T18072	pobj	MNase,of
R5212	T18074	T18075	punct	(,units
R5213	T18075	T18073	parataxis	units,MNase
R5214	T18076	T18077	quantmod	0.04,1.6
R5215	T18077	T18075	nummod	1.6,units
R5216	T18078	T18077	punct	–,1.6
R5217	T18079	T18075	punct	),units
R5218	T18080	T18073	cc	or,MNase
R5219	T18081	T18073	conj	DNase,MNase
R5220	T18082	T18081	nummod	I,DNase
R5221	T18083	T18084	punct	(,units
R5222	T18084	T18081	parataxis	units,DNase
R5223	T18085	T18086	quantmod	0.25,16
R5224	T18086	T18084	nummod	16,units
R5225	T18087	T18086	punct	–,16
R5226	T18088	T18084	punct	),units
R5227	T18089	T18067	punct	.,incubated
R5228	T18091	T18092	compound	Digestion,time
R5229	T18092	T18093	nsubj	time,was
R5230	T18094	T18092	prep	at,time
R5231	T18095	T18096	nummod	37,°C
R5232	T18096	T18094	pobj	°C,at
R5233	T18097	T18098	nummod	10,min
R5234	T18098	T18093	attr	min,was
R5235	T18099	T18093	prep	for,was
R5236	T18100	T18099	pobj	MNase,for
R5237	T18101	T18093	cc	and,was
R5238	T18102	T18103	nummod	2,min
R5239	T18103	T18093	conj	min,was
R5240	T18104	T18103	prep	for,min
R5241	T18105	T18104	pobj	DNase,for
R5242	T18106	T18105	nummod	I,DNase
R5243	T18107	T18093	punct	.,was
R5244	T18109	T18110	nsubjpass	Digestions,stopped
R5245	T18111	T18110	auxpass	were,stopped
R5246	T18112	T18110	prep	by,stopped
R5247	T18113	T18112	pcomp	adding,by
R5248	T18114	T18113	dobj	SDS,adding
R5249	T18115	T18113	prep	to,adding
R5250	T18116	T18117	nummod	1,%
R5251	T18117	T18115	pobj	%,to
R5252	T18118	T18113	cc	and,adding
R5253	T18119	T18113	conj	EDTA,adding
R5254	T18120	T18119	prep	to,EDTA
R5255	T18121	T18122	nummod	50,mM
R5256	T18122	T18120	pobj	mM,to
R5257	T18123	T18110	punct	.,stopped
R5258	T18125	T18126	nsubjpass	DNAs,prepared
R5259	T18127	T18126	auxpass	were,prepared
R5260	T18128	T18126	prep	by,prepared
R5261	T18129	T18130	compound	proteinase,K
R5262	T18130	T18131	compound	K,digestion
R5263	T18131	T18128	pobj	digestion,by
R5264	T18132	T18131	acl	followed,digestion
R5265	T18133	T18132	agent	by,followed
R5266	T18134	T18135	compound	phenol,chloroform
R5267	T18135	T18137	compound	chloroform,extraction
R5268	T18136	T18135	punct	-,chloroform
R5269	T18137	T18133	pobj	extraction,by
R5270	T18138	T18137	cc	and,extraction
R5271	T18139	T18140	compound	isopropanol,precipitation
R5272	T18140	T18137	conj	precipitation,extraction
R5273	T18141	T18126	punct	.,prepared
R5274	T18143	T18144	aux	To,test
R5275	T18144	T18145	advcl	test,incubated
R5276	T18146	T18147	det	the,association
R5277	T18147	T18144	dobj	association,test
R5278	T18148	T18147	prep	of,association
R5279	T18149	T18150	compound	histone,H3
R5280	T18150	T18148	pobj	H3,of
R5281	T18151	T18147	prep	with,association
R5282	T18152	T18151	pobj	chromatin,with
R5283	T18153	T18144	prep	in,test
R5284	T18154	T18155	nmod	control,cells
R5285	T18155	T18153	pobj	cells,in
R5286	T18156	T18154	cc	and,control
R5287	T18157	T18158	npadvmod	p150,depleted
R5288	T18158	T18154	conj	depleted,control
R5289	T18159	T18158	punct	-,depleted
R5290	T18160	T18145	punct	", ",incubated
R5291	T18161	T18162	amod	isolated,nuclei
R5292	T18162	T18145	nsubjpass	nuclei,incubated
R5293	T18163	T18145	auxpass	were,incubated
R5294	T18164	T18145	prep	on,incubated
R5295	T18165	T18164	pobj	ice,on
R5296	T18166	T18145	prep	for,incubated
R5297	T18167	T18168	nummod	30,min
R5298	T18168	T18166	pobj	min,for
R5299	T18169	T18145	prep	in,incubated
R5300	T18170	T18169	pobj	buffer,in
R5301	T18171	T18170	nummod	2,buffer
R5302	T18172	T18173	punct	(,Hepes
R5303	T18173	T18170	parataxis	Hepes,buffer
R5304	T18174	T18175	nummod	50,mM
R5305	T18175	T18173	compound	mM,Hepes
R5306	T18176	T18177	punct	[,pH7.9
R5307	T18177	T18173	parataxis	pH7.9,Hepes
R5308	T18178	T18177	punct	],pH7.9
R5309	T18179	T18173	punct	", ",Hepes
R5310	T18180	T18181	nummod	20,%
R5311	T18181	T18182	compound	%,Glycerol
R5312	T18182	T18173	appos	Glycerol,Hepes
R5313	T18183	T18173	punct	", ",Hepes
R5314	T18184	T18185	nummod	3,mM
R5315	T18185	T18186	compound	mM,MgCl2
R5316	T18186	T18173	appos	MgCl2,Hepes
R5317	T18187	T18173	punct	", ",Hepes
R5318	T18188	T18189	nummod	0.1,%
R5319	T18189	T18190	compound	%,IGEPAL
R5320	T18190	T18173	appos	IGEPAL,Hepes
R5321	T18191	T18173	punct	", ",Hepes
R5322	T18192	T18193	nummod	0.5,mM
R5323	T18193	T18194	compound	mM,DTT
R5324	T18194	T18173	appos	DTT,Hepes
R5325	T18195	T18173	punct	", ",Hepes
R5326	T18196	T18197	nummod	0.5,mM
R5327	T18197	T18198	compound	mM,PMSF
R5328	T18198	T18173	appos	PMSF,Hepes
R5329	T18199	T18173	punct	),Hepes
R5330	T18200	T18170	acl	supplemented,buffer
R5331	T18201	T18200	prep	with,supplemented
R5332	T18202	T18203	preconj	either,M
R5333	T18203	T18205	nmod	M,NaCl
R5334	T18204	T18203	nummod	0.1,M
R5335	T18205	T18201	pobj	NaCl,with
R5336	T18206	T18203	punct	", ",M
R5337	T18207	T18208	nummod	0.3,M
R5338	T18208	T18203	conj	M,M
R5339	T18209	T18208	punct	", ",M
R5340	T18210	T18211	nummod	0.45,M
R5341	T18211	T18208	conj	M,M
R5342	T18212	T18211	punct	", ",M
R5343	T18213	T18214	nummod	0.7,M
R5344	T18214	T18211	conj	M,M
R5345	T18215	T18214	punct	", ",M
R5346	T18216	T18214	cc	or,M
R5347	T18217	T18218	nummod	1,M
R5348	T18218	T18214	conj	M,M
R5349	T18219	T18145	punct	.,incubated
R5350	T18221	T18222	prep	After,analyzed
R5351	T18223	T18221	pobj	centrifugation,After
R5352	T18224	T18223	prep	at,centrifugation
R5353	T18225	T18226	nummod	"15,000",g
R5354	T18226	T18224	pobj	g,at
R5355	T18227	T18222	punct	", ",analyzed
R5356	T18228	T18222	nsubjpass	pellets,analyzed
R5357	T18229	T18228	cc	and,pellets
R5358	T18230	T18228	conj	supernants,pellets
R5359	T18231	T18222	auxpass	were,analyzed
R5360	T18232	T18222	prep	by,analyzed
R5361	T18233	T18234	compound	Western,blot
R5362	T18234	T18232	pobj	blot,by
R5363	T18235	T18222	advcl	using,analyzed
R5364	T18236	T18237	det	a,antibody
R5365	T18237	T18235	dobj	antibody,using
R5366	T18238	T18239	compound	histone,H3
R5367	T18239	T18237	compound	H3,antibody
R5368	T18240	T18241	punct	(,Abcam
R5369	T18241	T18237	parataxis	Abcam,antibody
R5370	T18242	T18241	punct	),Abcam
R5371	T18243	T18222	punct	.,analyzed
R5372	T18632	T18631	punct	.,ChIP
R5373	T18634	T18635	nsubj	We,prepared
R5374	T18636	T18637	amod	native,fragments
R5375	T18637	T18635	dobj	fragments,prepared
R5376	T18638	T18637	compound	chromatin,fragments
R5377	T18639	T18637	prep	of,fragments
R5378	T18640	T18641	quantmod	two,six
R5379	T18641	T18643	nummod	six,nucleosomes
R5380	T18642	T18641	quantmod	to,six
R5381	T18643	T18639	pobj	nucleosomes,of
R5382	T18644	T18643	prep	in,nucleosomes
R5383	T18645	T18644	pobj	length,in
R5384	T18646	T18647	mark	as,described
R5385	T18647	T18635	advcl	described,prepared
R5386	T18648	T18649	punct	[,28
R5387	T18649	T18635	parataxis	28,prepared
R5388	T18650	T18649	punct	],28
R5389	T18651	T18635	punct	.,prepared
R5390	T18653	T18654	nummod	5,μg
R5391	T18654	T18655	nsubjpass	μg,incubated
R5392	T18656	T18654	prep	of,μg
R5393	T18657	T18656	pobj	chromatin,of
R5394	T18658	T18655	auxpass	were,incubated
R5395	T18659	T18655	advmod	overnight,incubated
R5396	T18660	T18655	prep	with,incubated
R5397	T18661	T18662	nummod	10,μl
R5398	T18662	T18660	pobj	μl,with
R5399	T18663	T18662	prep	of,μl
R5400	T18664	T18665	amod	commercial,antibodies
R5401	T18665	T18663	pobj	antibodies,of
R5402	T18666	T18655	punct	.,incubated
R5403	T18668	T18669	prep	After,purified
R5404	T18670	T18668	pobj	incubation,After
R5405	T18671	T18670	prep	with,incubation
R5406	T18672	T18673	compound	protein,G
R5407	T18673	T18675	compound	G,sepharose
R5408	T18674	T18673	punct	-,G
R5409	T18675	T18671	pobj	sepharose,with
R5410	T18676	T18670	cc	and,incubation
R5411	T18677	T18678	nummod	three,washes
R5412	T18678	T18670	conj	washes,incubation
R5413	T18679	T18669	punct	", ",purified
R5414	T18680	T18681	amod	immunoprecipitated,DNA
R5415	T18681	T18669	nsubjpass	DNA,purified
R5416	T18682	T18669	auxpass	was,purified
R5417	T18683	T18669	punct	", ",purified
R5418	T18684	T18669	conj	sized,purified
R5419	T18685	T18684	prep	onto,sized
R5420	T18686	T18687	det	an,gel
R5421	T18687	T18685	pobj	gel,onto
R5422	T18688	T18687	compound	agarose,gel
R5423	T18689	T18684	punct	", ",sized
R5424	T18690	T18684	cc	and,sized
R5425	T18691	T18684	conj	analyzed,sized
R5426	T18692	T18691	prep	by,analyzed
R5427	T18693	T18694	compound	Southern,blot
R5428	T18694	T18692	pobj	blot,by
R5429	T18695	T18669	punct	.,purified
R5430	T18697	T18698	compound	Hybridization,signals
R5431	T18698	T18699	nsubjpass	signals,quantified
R5432	T18700	T18699	auxpass	were,quantified
R5433	T18701	T18699	advcl	using,quantified
R5434	T18702	T18703	det	an,Imager
R5435	T18703	T18701	dobj	Imager,using
R5436	T18704	T18703	compound	Instant,Imager
R5437	T18705	T18706	punct	(,PerkinElmer
R5438	T18706	T18703	parataxis	PerkinElmer,Imager
R5439	T18707	T18706	punct	", ",PerkinElmer
R5440	T18708	T18706	npadvmod	Wellesley,PerkinElmer
R5441	T18709	T18706	punct	", ",PerkinElmer
R5442	T18710	T18706	npadvmod	California,PerkinElmer
R5443	T18711	T18706	punct	", ",PerkinElmer
R5444	T18712	T18713	compound	United,States
R5445	T18713	T18706	npadvmod	States,PerkinElmer
R5446	T18714	T18706	punct	),PerkinElmer
R5447	T18715	T18699	punct	.,quantified
R5448	T18717	T18718	nsubjpass	Antibodies,purchased
R5449	T18719	T18717	amod	specific,Antibodies
R5450	T18720	T18717	prep	for,Antibodies
R5451	T18721	T18720	pobj	H4K20me3,for
R5452	T18722	T18718	auxpass	were,purchased
R5453	T18723	T18718	prep	from,purchased
R5454	T18724	T18723	pobj	Abcam,from
R5455	T18725	T18718	punct	.,purchased
R5456	T18727	T18728	nsubjpass	Antibodies,purchased
R5457	T18729	T18727	prep	for,Antibodies
R5458	T18730	T18729	pobj	H3K9me3,for
R5459	T18731	T18728	auxpass	were,purchased
R5460	T18732	T18728	prep	from,purchased
R5461	T18733	T18734	preconj	both,Abcam
R5462	T18734	T18732	pobj	Abcam,from
R5463	T18735	T18734	cc	and,Abcam
R5464	T18736	T18737	compound	Upstate,Biotechnology
R5465	T18737	T18734	conj	Biotechnology,Abcam
R5466	T18738	T18728	cc	and,purchased
R5467	T18739	T18728	conj	gave,purchased
R5468	T18740	T18741	amod	similar,results
R5469	T18741	T18739	dobj	results,gave
R5470	T18742	T18728	punct	.,purchased
R5471	T18744	T18745	prep	For,used
R5472	T18746	T18747	compound	Southern,blot
R5473	T18747	T18748	compound	blot,analysis
R5474	T18748	T18744	pobj	analysis,For
R5475	T18749	T18745	punct	", ",used
R5476	T18750	T18745	nsubj	we,used
R5477	T18751	T18752	det	a,fragment
R5478	T18752	T18745	dobj	fragment,used
R5479	T18753	T18754	nummod	240,bp
R5480	T18754	T18752	compound	bp,fragment
R5481	T18755	T18754	punct	-,bp
R5482	T18756	T18757	compound	EcoRI,BamHI
R5483	T18757	T18752	compound	BamHI,fragment
R5484	T18758	T18757	punct	/,BamHI
R5485	T18759	T18752	prep	from,fragment
R5486	T18760	T18759	pobj	pSAT,from
R5487	T18761	T18762	punct	(,probe
R5488	T18762	T18760	parataxis	probe,pSAT
R5489	T18763	T18762	amod	major,probe
R5490	T18764	T18762	compound	satellite,probe
R5491	T18765	T18762	punct	),probe
R5492	T18766	T18767	punct	[,45
R5493	T18767	T18752	parataxis	45,fragment
R5494	T18768	T18769	punct	–,47
R5495	T18769	T18767	prep	47,45
R5496	T18770	T18767	punct	],45
R5497	T18771	T18752	cc	and,fragment
R5498	T18772	T18773	det	a,fragment
R5499	T18773	T18752	conj	fragment,fragment
R5500	T18774	T18775	nummod	360,bp
R5501	T18775	T18773	compound	bp,fragment
R5502	T18776	T18775	punct	-,bp
R5503	T18777	T18778	compound	EcoRI,HindIII
R5504	T18778	T18773	compound	HindIII,fragment
R5505	T18779	T18778	punct	/,HindIII
R5506	T18780	T18773	prep	from,fragment
R5507	T18781	T18780	pobj	R198,from
R5508	T18782	T18773	punct	", ",fragment
R5509	T18783	T18773	acl	corresponding,fragment
R5510	T18784	T18783	prep	to,corresponding
R5511	T18785	T18786	nummod	three,copies
R5512	T18786	T18784	pobj	copies,to
R5513	T18787	T18786	prep	of,copies
R5514	T18788	T18789	det	the,repeat
R5515	T18789	T18787	pobj	repeat,of
R5516	T18790	T18791	nummod	120,bp
R5517	T18791	T18789	nmod	bp,repeat
R5518	T18792	T18791	punct	-,bp
R5519	T18793	T18794	amod	minor,satellite
R5520	T18794	T18789	compound	satellite,repeat
R5521	T18795	T18796	punct	[,49
R5522	T18796	T18783	parataxis	49,corresponding
R5523	T18797	T18796	punct	],49
R5524	T18798	T18745	punct	.,used
R5525	T18800	T18801	nsubjpass	Probes,described
R5526	T18802	T18800	acl	used,Probes
R5527	T18803	T18802	prep	in,used
R5528	T18804	T18805	compound	FISH,experiments
R5529	T18805	T18803	pobj	experiments,in
R5530	T18806	T18801	auxpass	were,described
R5531	T18807	T18801	prep	in,described
R5532	T18808	T18807	punct	[,in
R5533	T18809	T18807	pobj	46,in
R5534	T18810	T18811	punct	–,48
R5535	T18811	T18809	prep	48,46
R5536	T18812	T18801	punct	],described
R5537	T18813	T18801	punct	.,described
R5538	T18815	T18816	det	The,probe
R5539	T18816	T18817	nsubjpass	probe,described
R5540	T18818	T18816	prep	for,probe
R5541	T18819	T18820	compound	IAP,elements
R5542	T18820	T18818	pobj	elements,for
R5543	T18821	T18817	auxpass	was,described
R5544	T18822	T18817	prep	in,described
R5545	T18823	T18822	punct	[,in
R5546	T18824	T18822	pobj	50,in
R5547	T18825	T18817	punct	],described
R5548	T18826	T18817	punct	.,described
R2936	T10332	T10327	punct	", ",detected
R2938	T10333	T10334	amod	residual,epitopes
R2940	T10334	T10327	nsubjpass	epitopes,detected
R2941	T10335	T10334	compound	H4K20me3,epitopes
R2942	T10336	T10327	auxpass	were,detected
R2944	T10337	T10327	prep	at,detected
R2945	T10338	T10339	det	the,level
R2947	T10339	T10337	pobj	level,at
R2948	T10340	T10339	prep	of,level
R2949	T10341	T10342	amod	relocated,material
R2951	T10342	T10340	pobj	material,of
R2952	T10343	T10344	npadvmod	DAPI,dense
R2953	T10344	T10342	amod	dense,material
R2954	T10345	T10344	punct	-,dense
R2956	T10346	T10327	punct	.,detected
R2957	T10348	T10349	advmod	Altogether,show
R2959	T10350	T10349	punct	", ",show
R2960	T10351	T10352	poss	our,results
R2961	T10352	T10349	nsubj	results,show
R2962	T10353	T10354	mark	that,leads
R2963	T10354	T10349	ccomp	leads,show
R2964	T10355	T10354	nsubj	depletion,leads
R2966	T10356	T10355	prep	of,depletion
R2967	T10357	T10356	pobj	CAF,of
R2968	T10358	T10357	punct	-,CAF
R2970	T10359	T10357	nummod	1,CAF
R2971	T10360	T10355	prep	in,depletion

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T712	0-5	GO:0033186	denotes	CAF-1
T713	23-38	GO:0000792	denotes	Heterochromatin
T714	55-66	_FRAGMENT	denotes	Pluripotent
T715	77-82	CL:0002248	denotes	Cells
T716	67-76	UBERON:0000922	denotes	Embryonic
T717	67-82	CL:0002321	denotes	Embryonic Cells
T718	77-82	CL_GO_EXT:cell	denotes	Cells
T719	136-145	NCBITaxon:40674	denotes	mammalian
T720	159-168	GO:0000785	denotes	chromatin
T721	182-200	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic marking
T722	193-200	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	marking
T723	219-225	SO_EXT:0001026	denotes	genome
T724	287-296	GO:0000785	denotes	chromatin
T725	287-305	GO:0031497	denotes	chromatin assembly
T726	356-374	GO:0031497	denotes	chromatin assembly
T727	356-383	GO:0033186	denotes	chromatin assembly factor 1
T728	385-390	GO:0033186	denotes	CAF-1
T729	403-415	GO:0007566	denotes	implantation
T730	444-449	NCBITaxon:10088	denotes	mouse
T731	475-483	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T732	491-495	SO_EXT:0000704	denotes	gene
T733	496-504	SO_EXT:sequence_coding_function	denotes	encoding
T734	524-533	PR_EXT:000005402	denotes	p150CAF-1
T735	543-552	PR_EXT:000005402	denotes	p150CAF-1
T736	567-574	SO_EXT:sequence_altered_entity	denotes	mutants
T737	615-619	CL_GO_EXT:cell	denotes	cell
T738	638-647	PR_EXT:000005402	denotes	p150CAF-1
T739	657-664	UBERON:0000922	denotes	embryos
T740	702-709	GO:0005634	denotes	nuclear
T741	739-754	GO:0000792	denotes	heterochromatin
T742	784-793	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T743	794-801	UBERON:0000922	denotes	embryos
T744	803-818	GO:0000792	denotes	heterochromatin
T745	867-875	_FRAGMENT	denotes	two-cell
T746	891-897	UBERON:0007232	denotes	stages
T747	871-875	CL_GO_EXT:cell	denotes	cell
T748	880-890	UBERON:0000358	denotes	blastocyst
T749	902-911	PR_EXT:000005402	denotes	p150CAF-1
T750	912-918	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T751	922-926	CL_GO_EXT:cell	denotes	cell
T752	933-940	UBERON:0000922	denotes	embryos
T753	970-985	GO:0000792	denotes	heterochromatin
T754	1028-1043	GO:0000792	denotes	heterochromatin
T755	1047-1051	_FRAGMENT	denotes	two-
T756	1060-1070	UBERON:0007232	denotes	cell stage
T757	1055-1070	UBERON:0007233	denotes	four-cell stage
T758	1060-1064	CL_GO_EXT:cell	denotes	cell
T759	1071-1080	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T760	1081-1088	UBERON:0000922	denotes	embryos
T761	1106-1111	GO:0033186	denotes	CAF-1
T762	1132-1142	GO_PATO_EXT:biological_maturation	denotes	maturation
T763	1146-1161	GO:0000792	denotes	heterochromatin
T764	1172-1184	GO:0007566	denotes	implantation
T765	1201-1210	UBERON:0000922	denotes	embryonic
T766	1201-1221	CL:0002322	denotes	embryonic stem cells
T767	1216-1221	CL_GO_EXT:cell	denotes	cells
T768	1236-1245	PR_EXT:000005402	denotes	p150CAF-1
T769	1252-1255	CHEBI_SO_EXT:RNA	denotes	RNA
T770	1252-1268	GO:0016246	denotes	RNA interference
T771	1287-1299	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T772	1343-1370	GO:0005721	denotes	pericentric heterochromatin
T773	1401-1406	GO:0033186	denotes	CAF-1
T774	1416-1421	CL_GO_EXT:cell	denotes	cells
T775	1451-1461	_FRAGMENT	denotes	epigenetic
T776	1482-1487	SO_EXT:epigenetic_modification_entity	denotes	marks
T777	1462-1469	PR_EXT:000043452	denotes	histone
T778	1462-1469	CHEBI:15358	denotes	histone
T779	1462-1481	GO:0016571	denotes	histone methylation
T780	1470-1481	GO_MOP_EXT:methylation	denotes	methylation
T781	1482-1487	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marks
T782	1504-1531	GO:0005721	denotes	pericentric heterochromatin
T783	1539-1550	SO_EXT:sequence_alteration_entity_or_process	denotes	alterations
T784	1554-1569	GO:0000792	denotes	heterochromatin
T785	1587-1596	PR_EXT:000005402	denotes	p150CAF-1
T786	1606-1611	NCBITaxon:10088	denotes	mouse
T787	1612-1621	UBERON:0000922	denotes	embryonic
T788	1622-1633	CL:0000057	denotes	fibroblasts
T789	1645-1650	CL_GO_EXT:cell	denotes	cells
T790	1703-1708	GO:0033186	denotes	CAF-1
T791	1738-1753	GO:0000792	denotes	heterochromatin
T792	1770-1781	_FRAGMENT	denotes	pluripotent
T793	1792-1797	CL:0002248	denotes	cells
T794	1782-1791	UBERON:0000922	denotes	embryonic
T795	1782-1797	CL:0002321	denotes	embryonic cells
T796	1792-1797	CL_GO_EXT:cell	denotes	cells
T797	1838-1847	GO:0000785	denotes	chromatin
T798	1838-1856	GO:0031497	denotes	chromatin assembly
T799	1870-1881	GO:0065007	denotes	controlling
T800	1911-1929	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic marking
T801	1922-1929	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	marking
T802	1937-1943	SO_EXT:0001026	denotes	genome
T803	1947-1960	UBERON:0019248	denotes	early embryos
T804	1965-1974	UBERON:0000922	denotes	embryonic
T805	1965-1985	CL:0002322	denotes	embryonic stem cells
T806	1980-1985	CL_GO_EXT:cell	denotes	cells
T2162	3244-3249	NCBITaxon:10088	denotes	mouse
T2163	3254-3266	GO:0007566	denotes	implantation
T2164	3284-3290	SO_EXT:0001026	denotes	genome
T2165	3319-3337	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic changes
T2166	3351-3360	UBERON:0000922	denotes	embryonic
T2167	3361-3365	SO_EXT:0000704	denotes	gene
T2168	3361-3376	GO:0010467	denotes	gene expression
T2169	3424-3439	GO_RO_EXT:developmental_differentiation_process	denotes	differentiation
T2170	3508-3511	CHEBI_SO_EXT:DNA	denotes	DNA
T2171	3508-3523	GO:0006306	denotes	DNA methylation
T2172	3512-3523	GO_MOP_EXT:methylation	denotes	methylation
T2173	3527-3537	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic
T2174	3592-3601	GO:0000785	denotes	chromatin
T2175	3667-3674	PR_EXT:000043452	denotes	histone
T2176	3667-3674	CHEBI:15358	denotes	histone
T2177	3667-3684	GO:0016570	denotes	histone modifying
T2178	3675-3684	SO_EXT:sequence_alteration_process	denotes	modifying
T2179	3685-3692	CHEBI_GO_SO_EXT:enzyme	denotes	enzymes
T2180	3694-3703	GO:0000785	denotes	chromatin
T2181	3694-3714	GO:0006338	denotes	chromatin remodeling
T2182	3728-3735	PR_EXT:000043452	denotes	histone
T2183	3728-3735	CHEBI:15358	denotes	histone
T2184	3758-3770	GO:0007566	denotes	implantation
T2185	3790-3800	CL:0000034	denotes	stem cells
T2186	3795-3800	CL_GO_EXT:cell	denotes	cells
T2187	3814-3827	UBERON:0019248	denotes	early embryos
T2188	3856-3863	PR_EXT:000043452	denotes	histone
T2189	3856-3863	CHEBI:15358	denotes	histone
T2190	3879-3888	GO:0000785	denotes	chromatin
T2191	3879-3899	GO:0006338	denotes	chromatin remodeling
T2192	3900-3909	GO:0032991	denotes	complexes
T2193	3937-3947	GO:0000786	denotes	nucleosome
T2194	3937-3956	GO:0006334	denotes	nucleosome assembly
T2195	4011-4018	GO:0065007	denotes	control
T2196	4019-4028	GO:0000785	denotes	chromatin
T2197	4019-4037	GO:0031497	denotes	chromatin assembly
T2198	4042-4052	GO_PATO_EXT:biological_maturation	denotes	maturation
T2199	4085-4092	PR_EXT:000043452	denotes	histone
T2200	4085-4092	CHEBI:15358	denotes	histone
T2201	4105-4123	GO:0031497	denotes	chromatin assembly
T2202	4105-4132	GO:0033186	denotes	chromatin assembly factor 1
T2203	4134-4139	GO:0033186	denotes	CAF-1
T2204	4161-4165	PR_EXT:000005402	denotes	p150
T2205	4167-4170	PR_EXT:000005403	denotes	p60
T2206	4176-4179	PR_EXT:000013776	denotes	p48
T2207	4181-4188	GO:0032991	denotes	complex
T2208	4204-4211	CHEBI:15358	denotes	histone
T2209	4204-4214	PR_EXT:000027594	denotes	histone H3
T2210	4204-4211	_FRAGMENT	denotes	histone
T2211	4219-4221	PR_EXT:000008603	denotes	H4
T2212	4256-4259	CHEBI_SO_EXT:DNA	denotes	DNA
T2213	4267-4278	GO:0006260	denotes	replication
T2214	4282-4285	CHEBI_SO_EXT:DNA	denotes	DNA
T2215	4282-4292	GO:0006281	denotes	DNA repair
T2216	4315-4320	GO:0033186	denotes	CAF-1
T2217	4334-4341	CHEBI:15358	denotes	histone
T2218	4334-4344	PR_EXT:000027594	denotes	histone H3
T2219	4345-4352	SO_EXT:sequence_variant_or_variation	denotes	variant
T2220	4363-4372	GO:0000785	denotes	chromatin
T2221	4398-4401	CHEBI_SO_EXT:DNA	denotes	DNA
T2222	4398-4411	GO:0071897	denotes	DNA synthesis
T2223	4430-4437	PR_EXT:000043452	denotes	histone
T2224	4430-4437	CHEBI:15358	denotes	histone
T2225	4449-4453	PR_EXT:000008570	denotes	HIRA
T2226	4492-4496	PR_EXT:000008425	denotes	H3.3
T2227	4497-4504	SO_EXT:sequence_variant_entity	denotes	variant
T2228	4510-4513	CHEBI_SO_EXT:DNA	denotes	DNA
T2229	4510-4523	GO:0071897	denotes	DNA synthesis
T2230	4582-4591	GO:0000785	denotes	chromatin
T2231	4582-4600	GO:0031497	denotes	chromatin assembly
T2232	4611-4616	GO:0033186	denotes	CAF-1
T2233	4640-4648	CHEBI_PR_EXT:protein	denotes	proteins
T2234	4660-4675	GO:0000792	denotes	heterochromatin
T2235	4687-4702	GO:0000792	denotes	heterochromatin
T2236	4687-4712	PR_EXT:P05205	denotes	heterochromatin protein 1
T2237	4703-4710	CHEBI_PR_EXT:protein	denotes	protein
T2238	4714-4717	PR_EXT:P05205	denotes	HP1
T2239	4723-4727	PR_EXT:000010214	denotes	MBD1
T2240	4742-4749	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T2241	4750-4756	SO_EXT:0000417	denotes	domain
T2242	4757-4764	CHEBI_PR_EXT:protein	denotes	protein
T2243	4770-4778	GO_EXT:biological_recruitment	denotes	recruits
T2244	4779-4786	PR_EXT:000043452	denotes	histone
T2245	4779-4786	CHEBI:15358	denotes	histone
T2246	4779-4798	GO_EXT:0004407	denotes	histone deacetylase
T2247	4814-4821	PR_EXT:000043452	denotes	histone
T2248	4814-4821	CHEBI:15358	denotes	histone
T2249	4814-4839	GO_EXT:0042054	denotes	histone methyltransferase
T2250	4873-4882	PR_EXT:000005402	denotes	p150CAF-1
T2251	4907-4918	GO:0006260	denotes	replication
T2252	4936-4939	PR_EXT:P05205	denotes	HP1
T2253	4940-4949	CHEBI:36357	denotes	molecules
T2254	4953-4964	GO:0006260	denotes	replication
T2255	4953-4970	SO_EXT:0000296	denotes	replication sites
T2256	4974-5001	GO:0005721	denotes	pericentric heterochromatin
T2257	5018-5025	GO:0051320	denotes	S phase
T2258	5077-5082	GO:0033186	denotes	CAF-1
T2259	5090-5118	GO:0031507	denotes	formation of heterochromatin
T2260	5103-5118	GO:0000792	denotes	heterochromatin
T2261	5146-5156	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic
T2262	5192-5214	SO_EXT:biological_conservation_process_or_quality	denotes	evolutionary conserved
T2263	5215-5222	PR_EXT:000043452	denotes	histone
T2264	5215-5222	CHEBI:15358	denotes	histone
T2265	5262-5275	CHEBI_EXT:biochemical	denotes	biochemically
T2266	5357-5364	NCBITaxon:40674	denotes	mammals
T2267	5372-5389	CL:0002248	denotes	pluripotent cells
T2268	5384-5389	CL_GO_EXT:cell	denotes	cells
T2269	5398-5407	UBERON:0000922	denotes	embryonic
T2270	5398-5412	_FRAGMENT	denotes	embryonic stem
T2271	5418-5423	CL:0002322	denotes	cells
T2272	5414-5416	_FRAGMENT	denotes	ES
T2273	5418-5423	CL_GO_EXT:cell	denotes	cells
T2274	5466-5471	GO:0033186	denotes	CAF-1
T2275	5485-5490	NCBITaxon:10088	denotes	mouse
T2276	5506-5513	SO_EXT:0000704	denotes	genetic
T2277	5530-5538	CL:0002322	denotes	ES cells
T2278	5533-5538	CL_GO_EXT:cell	denotes	cells
T2279	5558-5561	CHEBI_SO_EXT:RNA	denotes	RNA
T2280	5558-5574	GO:0016246	denotes	RNA interference
T2281	5576-5580	GO:0016246	denotes	RNAi
T2282	5596-5601	GO:0033186	denotes	CAF-1
T2283	5632-5637	_FRAGMENT	denotes	early
T2284	5644-5651	UBERON:0019248	denotes	embryos
T2285	5638-5643	NCBITaxon:10088	denotes	mouse
T2286	5656-5664	CL:0002322	denotes	ES cells
T2287	5659-5664	CL_GO_EXT:cell	denotes	cells
T2288	5691-5696	GO:0033186	denotes	CAF-1
T2289	5742-5760	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic marking
T2290	5753-5760	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	marking
T2291	5764-5779	GO:0000792	denotes	heterochromatin
T2292	5791-5802	_FRAGMENT	denotes	pluripotent
T2293	5813-5818	CL:0002248	denotes	cells
T2294	5803-5812	UBERON:0000922	denotes	embryonic
T2295	5803-5818	CL:0002321	denotes	embryonic cells
T2296	5813-5818	CL_GO_EXT:cell	denotes	cells
T7538	5837-5841	SO_EXT:0000704	denotes	gene
T7539	5855-5863	CL:0002322	denotes	ES cells
T7540	5858-5863	CL_GO_EXT:cell	denotes	cells
T7541	5867-5873	SO_EXT:sequence_deletion_process	denotes	delete
T7542	5874-5878	SO_EXT:0000147	denotes	exon
T7543	5888-5894	PR_EXT:000005402	denotes	Chaf1a
T7544	5895-5899	SO_EXT:0000704	denotes	gene
T7545	5907-5914	SO_EXT:sequence_coding_function	denotes	encodes
T7546	5915-5924	PR_EXT:000005402	denotes	p150CAF-1
T7547	5938-5944	PR_EXT:000005402	denotes	Chaf1a
T7548	5945-5946	SO_EXT:normal_or_wild_type_or_present	denotes	+
T7549	5947-5948	SO_EXT:sequence_nullness_or_absence	denotes	−
T7550	5949-5953	NCBITaxon:10088	denotes	mice
T7551	5959-5963	GO:0007567	denotes	born
T7552	6098-6102	NCBITaxon:10088	denotes	mice
T7553	6129-6136	PATO_UBERON_EXT:neonate_or_newborn	denotes	newborn
T7554	6137-6143	PR_EXT:000005402	denotes	Chaf1a
T7555	6144-6145	SO_EXT:sequence_nullness_or_absence	denotes	−
T7556	6146-6147	SO_EXT:sequence_nullness_or_absence	denotes	−
T7557	6148-6152	NCBITaxon:10088	denotes	mice
T7558	6181-6187	PR_EXT:000005402	denotes	Chaf1a
T7559	6188-6189	SO_EXT:sequence_nullness_or_absence	denotes	−
T7560	6190-6191	SO_EXT:sequence_nullness_or_absence	denotes	−
T7561	6192-6199	UBERON:0000922	denotes	embryos
T7562	6233-6245	GO:0007566	denotes	implantation
T7563	6279-6285	PR_EXT:000005402	denotes	Chaf1a
T7564	6286-6287	SO_EXT:sequence_nullness_or_absence	denotes	−
T7565	6288-6289	SO_EXT:sequence_nullness_or_absence	denotes	−
T7566	6290-6297	UBERON:0000922	denotes	embryos
T7567	6301-6310	UBERON:0000922	denotes	embryonic
T7568	6386-6393	UBERON:0000922	denotes	embryos
T7569	6408-6414	PR_EXT:000005402	denotes	Chaf1a
T7570	6415-6416	SO_EXT:normal_or_wild_type_or_present	denotes	+
T7571	6417-6418	SO_EXT:sequence_nullness_or_absence	denotes	−
T7572	6419-6423	NCBITaxon:10088	denotes	mice
T7573	6487-6493	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T7574	6494-6501	UBERON:0000922	denotes	embryos
T7575	6547-6553	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T7576	6554-6561	UBERON:0000922	denotes	embryos
T7577	6589-6594	CL_GO_EXT:cell	denotes	cells
T7578	6626-6631	CL_GO_EXT:cell	denotes	cells
T7579	6645-6654	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7580	6671-6682	UBERON:0000358	denotes	blastocysts
T7581	6792-6801	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7582	6806-6815	PR_EXT:000005402	denotes	p150CAF-1
T7583	6825-6832	UBERON:0000922	denotes	embryos
T7584	6844-6853	PR_EXT:000005402	denotes	p150CAF-1
T7585	6896-6912	UBERON:0007236	denotes	eight-cell stage
T7586	6902-6906	CL_GO_EXT:cell	denotes	cell
T7587	6971-6978	CHEBI_PR_EXT:protein	denotes	protein
T7588	6997-7003	UBERON:0000922	denotes	embryo
T7589	7014-7029	UBERON:0007233	denotes	four-cell stage
T7590	7019-7023	CL_GO_EXT:cell	denotes	cell
T7591	7050-7059	PR_EXT:000005402	denotes	p150CAF-1
T7592	7060-7067	CHEBI_PR_EXT:protein	denotes	protein
T7593	7119-7123	CL_GO_EXT:cell	denotes	cell
T7594	7119-7133	GO:0051301	denotes	cell divisions
T7595	9755-9762	GO:0005634	denotes	nuclear
T7596	9779-9794	GO:0000792	denotes	heterochromatin
T7597	9820-9826	GO:0005634	denotes	nuclei
T7598	9830-9836	PR_EXT:000005402	denotes	Chaf1a
T7599	9837-9838	SO_EXT:sequence_nullness_or_absence	denotes	−
T7600	9839-9840	SO_EXT:sequence_nullness_or_absence	denotes	−
T7601	9841-9848	UBERON:0000922	denotes	embryos
T7602	9850-9877	GO:0005721	denotes	Pericentric heterochromatin
T7603	9915-9930	GO:0000792	denotes	heterochromatin
T7604	9960-9970	GO:0051325	denotes	interphase
T7605	9971-9977	GO:0005634	denotes	nuclei
T7606	9985-9997	CHEBI_EXT:51217	denotes	fluorochrome
T7607	9998-10002	CHEBI:51231	denotes	DAPI
T7608	10030-10034	PR_EXT:000005086	denotes	HP1α
T7609	10050-10059	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7610	10060-10071	UBERON:0000358	denotes	blastocysts
T7611	10079-10084	NCBITaxon:10088	denotes	mouse
T7613	10093-10098	CL_GO_EXT:cell	denotes	cells
T7614	10100-10127	GO:0005721	denotes	pericentric heterochromatin
T7615	10175-10184	GO:0000785	denotes	chromatin
T7616	10203-10216	GO:0010369	denotes	chromocenters
T7617	10247-10251	CHEBI:51231	denotes	DAPI
T7618	10314-10320	PR_EXT:000005402	denotes	Chaf1a
T7619	10321-10322	SO_EXT:sequence_nullness_or_absence	denotes	−
T7620	10323-10324	SO_EXT:sequence_nullness_or_absence	denotes	−
T7621	10325-10332	UBERON:0000922	denotes	embryos
T7622	10352-10361	GO:0000785	denotes	chromatin
T7623	10421-10427	SO_EXT:0001026	denotes	genome
T7624	10545-10560	GO:0000792	denotes	heterochromatin
T7625	10586-10594	_FRAGMENT	denotes	two-cell
T7626	10610-10616	UBERON:0007232	denotes	stages
T7627	10590-10594	CL_GO_EXT:cell	denotes	cell
T7628	10599-10609	UBERON:0000358	denotes	blastocyst
T7629	10621-10635	UBERON:0007232	denotes	two-cell stage
T7630	10625-10629	CL_GO_EXT:cell	denotes	cell
T7631	10636-10645	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7632	10646-10653	UBERON:0000922	denotes	embryos
T7633	10655-10659	CHEBI:51231	denotes	DAPI
T7634	10737-10763	GO:0005731	denotes	nucleolar precursor bodies
T7635	10777-10792	GO:0000792	denotes	Heterochromatin
T7636	10834-10838	CHEBI:51231	denotes	DAPI
T7637	10862-10871	_FRAGMENT	denotes	four-cell
T7638	10895-10901	UBERON:0007233	denotes	stages
T7639	10867-10871	CL_GO_EXT:cell	denotes	cell
T7640	10879-10883	CL_GO_EXT:cell	denotes	cell
T7641	10884-10894	UBERON:0000358	denotes	blastocyst
T7642	10931-10935	PR_EXT:000005086	denotes	HP1α
T7643	10980-10984	CHEBI:51231	denotes	DAPI
T7644	11021-11036	GO:0000792	denotes	heterochromatin
T7645	11060-11067	GO:0005634	denotes	nuclear
T7646	11084-11099	GO:0000792	denotes	heterochromatin
T7647	11136-11148	GO:0007566	denotes	implantation
T7648	11174-11182	_FRAGMENT	denotes	two-cell
T7649	11198-11204	UBERON:0007232	denotes	stages
T7650	11178-11182	CL_GO_EXT:cell	denotes	cell
T7651	11187-11197	UBERON:0000358	denotes	blastocyst
T7652	11209-11215	PR_EXT:000005402	denotes	Chaf1a
T7653	11216-11217	SO_EXT:sequence_nullness_or_absence	denotes	−
T7654	11218-11219	SO_EXT:sequence_nullness_or_absence	denotes	−
T7655	11223-11230	UBERON:0000922	denotes	embryos
T7656	11263-11269	_FRAGMENT	denotes	eight-
T7657	11277-11287	UBERON:0007236	denotes	cell stage
T7658	11277-11281	CL_GO_EXT:cell	denotes	cell
T7659	11289-11293	CHEBI:51231	denotes	DAPI
T7660	11350-11354	CHEBI:51231	denotes	DAPI
T7661	11387-11394	GO:0005634	denotes	nucleus
T7662	11438-11446	GO:0005730	denotes	nucleoli
T7663	11458-11470	_FRAGMENT	denotes	periphery of
T7664	11475-11481	GO:0034399	denotes	nuclei
T7665	11495-11507	GO_PATO_EXT:biological_localization_process_or_quality	denotes	Localization
T7666	11511-11515	PR_EXT:000005086	denotes	HP1α
T7667	11556-11560	CHEBI:51231	denotes	DAPI
T7668	11590-11607	GO:0034399	denotes	nuclear periphery
T7669	11624-11632	GO:0005730	denotes	nucleoli
T7670	11676-11691	GO:0000792	denotes	heterochromatin
T7671	11695-11701	PR_EXT:000005402	denotes	Chaf1a
T7672	11702-11703	SO_EXT:sequence_nullness_or_absence	denotes	−
T7673	11704-11705	SO_EXT:sequence_nullness_or_absence	denotes	−
T7674	11706-11713	UBERON:0000922	denotes	embryos
T7675	11736-11751	GO:0000792	denotes	heterochromatin
T7676	11774-11778	_FRAGMENT	denotes	two-
T7677	11787-11797	UBERON:0007232	denotes	cell stage
T7678	11782-11797	UBERON:0007233	denotes	four-cell stage
T7679	11787-11791	CL_GO_EXT:cell	denotes	cell
T7680	11798-11807	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7681	11808-11815	UBERON:0000922	denotes	embryos
T7682	11879-11888	PR_EXT:000005402	denotes	p150CAF-1
T7683	11900-11912	GO:0007566	denotes	implantation
T7684	11947-11954	CHEBI_PR_EXT:protein	denotes	protein
T7685	12002-12024	_FRAGMENT	denotes	heterochromatin within
T7686	12040-12046	GO:0005720	denotes	nuclei
T7687	12025-12034	UBERON:0000922	denotes	embryonic
T7688	12025-12039	CL:0002321	denotes	embryonic cell
T7689	12035-12046	GO:0005634	denotes	cell nuclei
T7690	12100-12105	GO:0033186	denotes	CAF-1
T7691	12132-12140	CL:0002322	denotes	ES cells
T7692	12135-12140	CL_GO_EXT:cell	denotes	cells
T7693	12169-12179	UBERON:0000358	denotes	blastocyst
T7694	12180-12195	UBERON:0000087	denotes	inner cell mass
T7695	12186-12190	CL_GO_EXT:cell	denotes	cell
T7696	12246-12252	PR_EXT:000005402	denotes	Chaf1a
T7697	12253-12254	SO_EXT:sequence_nullness_or_absence	denotes	−
T7698	12255-12256	SO_EXT:sequence_nullness_or_absence	denotes	−
T7699	12257-12264	UBERON:0000922	denotes	embryos
T7700	12271-12276	CL_GO_EXT:cell	denotes	cells
T7701	12312-12316	SO_EXT:sequence_nullness	denotes	null
T7702	12317-12324	UBERON:0000922	denotes	embryos
T7703	12346-12350	GO:0016246	denotes	RNAi
T7704	12373-12382	PR_EXT:000005402	denotes	p150CAF-1
T7705	12396-12404	CL:0002322	denotes	ES cells
T7706	12399-12404	CL_GO_EXT:cell	denotes	cells
T7707	12466-12475	PR_EXT:000005402	denotes	p150CAF-1
T7708	12476-12480	GO:0016246	denotes	RNAi
T7709	12490-12498	CL:0002322	denotes	ES cells
T7710	12493-12498	CL_GO_EXT:cell	denotes	cells
T7711	12541-12546	CL_GO_EXT:cell	denotes	cells
T7712	12550-12556	PR_EXT:000005402	denotes	Chaf1a
T7713	12557-12558	SO_EXT:sequence_nullness_or_absence	denotes	−
T7714	12559-12560	SO_EXT:sequence_nullness_or_absence	denotes	−
T7715	12564-12568	NCBITaxon:10088	denotes	mice
T7716	12570-12574	CHEBI:51231	denotes	DAPI
T7717	12621-12625	PR_EXT:000005086	denotes	HP1α
T7718	12630-12634	CHEBI:51231	denotes	DAPI
T7719	12655-12663	GO:0005730	denotes	nucleoli
T7720	12675-12687	_FRAGMENT	denotes	periphery of
T7721	12692-12699	GO:0034399	denotes	nucleus
T7722	12740-12749	PR_EXT:000005402	denotes	p150CAF-1
T7723	12767-12774	GO:0005634	denotes	nuclear
T7724	12791-12806	GO:0000792	denotes	heterochromatin
T7725	12810-12818	CL:0002322	denotes	ES cells
T7726	12813-12818	CL_GO_EXT:cell	denotes	cells
T7727	12849-12861	GO:0007566	denotes	implantation
T7728	12862-12869	UBERON:0000922	denotes	embryos
T7729	12930-12945	GO:0000792	denotes	heterochromatin
T7730	12970-12975	NCBITaxon:10088	denotes	mouse
T7731	12976-12985	UBERON:0000922	denotes	embryonic
T7732	12986-12997	CL:0000057	denotes	fibroblasts
T7733	13015-13024	PR_EXT:000005402	denotes	p150CAF-1
T7734	13038-13042	GO:0016246	denotes	RNAi
T7735	13044-13053	PR_EXT:000005402	denotes	p150CAF-1
T7736	13107-13111	CL_GO_EXT:cell	denotes	cell
T7737	13107-13125	GO:0008283	denotes	cell proliferation
T7738	13182-13197	GO:0000792	denotes	heterochromatin
T7739	13238-13247	PR_EXT:000005402	denotes	p150CAF-1
T7740	13265-13270	CL_GO_EXT:cell	denotes	cells
T7741	13286-13301	GO:0000792	denotes	heterochromatin
T7742	13371-13376	GO:0033186	denotes	CAF-1
T7743	13384-13396	UBERON:0019248	denotes	early embryo
T7744	13401-13409	CL:0002322	denotes	ES cells
T7745	13404-13409	CL_GO_EXT:cell	denotes	cells
T7746	14716-14725	PR_EXT:000005402	denotes	p150CAF-1
T7747	14729-14737	CL:0002322	denotes	ES cells
T7748	14732-14737	CL_GO_EXT:cell	denotes	cells
T7749	14763-14778	GO:0000792	denotes	heterochromatin
T7750	14779-14786	GO:0005634	denotes	nuclear
T7751	14855-14862	GO:0005634	denotes	nuclear
T7752	14913-14921	GO:0042571	denotes	antibody
T7753	14934-14947	CL:0000836	denotes	promyelocytic
T7754	14934-14956	PR_EXT:000026474	denotes	promyelocytic leukemia
T7755	14958-14961	PR_EXT:000026474	denotes	PML
T7756	14963-14970	CHEBI_PR_EXT:protein	denotes	protein
T7757	14996-14999	PR_EXT:000026474	denotes	PML
T7758	14996-15014	GO:0016605	denotes	PML nuclear bodies
T7759	15048-15055	GO:0005634	denotes	nuclear
T7760	15154-15157	PR_EXT:000026474	denotes	PML
T7761	15154-15172	GO:0016605	denotes	PML nuclear bodies
T7762	15193-15202	PR_EXT:000005402	denotes	p150CAF-1
T7763	15212-15220	CL:0002322	denotes	ES cells
T7764	15215-15220	CL_GO_EXT:cell	denotes	cells
T7765	15255-15262	GO:0005634	denotes	nuclear
T7766	15310-15315	GO:0033186	denotes	CAF-1
T7767	15383-15388	GO:0033186	denotes	CAF-1
T7768	15435-15444	GO:0000785	denotes	chromatin
T7769	15459-15467	CHEBI_PR_EXT:protein	denotes	proteins
T7770	15476-15483	CHEBI:15358	denotes	histone
T7771	15476-15486	PR_EXT:000027594	denotes	histone H3
T7772	15491-15495	PR_EXT:000005086	denotes	HP1α
T7773	15515-15520	GO:0033186	denotes	CAF-1
T7774	15549-15562	UBERON:0019248	denotes	early embryos
T7775	15570-15578	CL:0002322	denotes	ES cells
T7776	15573-15578	CL_GO_EXT:cell	denotes	cells
T7777	15614-15629	GO:0000792	denotes	heterochromatin
T7778	15633-15640	GO:0005634	denotes	nuclear
T7779	15684-15693	NCBITaxon:40674	denotes	mammalian
T7780	15694-15698	CL_GO_EXT:cell	denotes	cell
T7781	15717-15722	GO:0033186	denotes	CAF-1
T7782	15748-15755	GO:0051320	denotes	S phase
T7783	15808-15820	GO:0009294	denotes	transfection
T7784	15828-15832	GO:0016246	denotes	RNAi
T7785	15833-15839	SO_EXT:0000440	denotes	vector
T7786	15841-15850	PR_EXT:000005402	denotes	p150CAF-1
T7787	15860-15865	CL_GO_EXT:cell	denotes	cells
T7788	15903-15908	GO:0033186	denotes	CAF-1
T7789	15944-15955	GO:0006260	denotes	replication
T7790	15993-16002	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymidine
T7791	16010-16027	CHEBI:472552	denotes	bromodeoxyuridine
T7792	16029-16033	CHEBI:472552	denotes	BrdU
T7793	16052-16056	PR_EXT:000012421	denotes	PCNA
T7794	16058-16071	GO:0008283	denotes	proliferating
T7795	16058-16092	PR_EXT:000012421	denotes	proliferating cell nuclear antigen
T7796	16072-16076	CL_GO_EXT:cell	denotes	cell
T7797	16085-16092	CHEBI_EXT:59132	denotes	antigen
T7798	16172-16177	CL_GO_EXT:cell	denotes	cells
T7799	16179-16182	CHEBI_SO_EXT:DNA	denotes	DNA
T7800	16179-16194	GO:0006260	denotes	DNA replication
T7801	16235-16250	GO:0000792	denotes	heterochromatin
T7802	16286-16294	CL:0002322	denotes	ES cells
T7803	16289-16294	CL_GO_EXT:cell	denotes	cells
T7804	16304-16315	GO:0008283	denotes	proliferate
T7805	16320-16323	GO:0016265	denotes	die
T7806	16340-16349	PR_EXT:000005402	denotes	p150CAF-1
T7807	16379-16387	CL_GO_EXT:cell	denotes	cellular
T7808	16379-16395	GO:0009987	denotes	cellular process
T7809	16403-16410	UBERON:0000922	denotes	embryos
T7810	16458-16467	PR_EXT:000005402	denotes	p150CAF-1
T7811	16480-16488	CL:0002322	denotes	ES cells
T7812	16483-16488	CL_GO_EXT:cell	denotes	cells
T7813	16493-16506	UBERON:0019248	denotes	early embryos
T7814	16540-16558	_FRAGMENT	denotes	heterochromatin in
T7815	16563-16570	GO:0005720	denotes	nucleus
T7816	16598-16602	CL_GO_EXT:cell	denotes	cell
T7817	16598-16608	GO:0007049	denotes	cell cycle
T7818	17892-17907	GO:0000792	denotes	heterochromatin
T7819	17945-17948	CHEBI_SO_EXT:DNA	denotes	DNA
T7820	17970-17983	GO:0097617	denotes	hybridization
T7821	18057-18084	GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation	denotes	centric chromosomal regions
T7822	18095-18100	NCBITaxon:10088	denotes	mouse
T7823	18156-18173	SO_EXT:satellite_unit_or_region	denotes	satellite repeats
T7824	18206-18218	_FRAGMENT	denotes	domains from
T7825	18229-18240	GO_SO_EXT:chromosomal_part_or_position_or_region_or_site	denotes	chromosomes
T7826	18317-18321	CHEBI:51231	denotes	DAPI
T7827	18340-18350	GO:0051325	denotes	interphase
T7828	18351-18358	GO:0005634	denotes	nucleus
T7829	18474-18483	PR_EXT:000005402	denotes	p150CAF-1
T7830	18484-18488	GO:0016246	denotes	RNAi
T7831	18498-18503	CL_GO_EXT:cell	denotes	cells
T7832	18533-18560	GO:0005721	denotes	pericentric heterochromatin
T7833	18611-18615	CHEBI:51231	denotes	DAPI
T7834	18663-18675	_FRAGMENT	denotes	domains from
T7835	18683-18694	GO_SO_EXT:chromosomal_part_or_position_or_region_or_site	denotes	chromosomes
T7836	18809-18814	CL_GO_EXT:cell	denotes	cells
T7837	18874-18891	GO:0034399	denotes	nuclear periphery
T7838	18914-18918	CHEBI:51231	denotes	DAPI
T7839	18954-18963	PR_EXT:000005402	denotes	p150CAF-1
T7840	19052-19059	GO:0005634	denotes	nucleus
T7841	19128-19132	CHEBI:51231	denotes	DAPI
T7842	19143-19152	SO_EXT:satellite_unit_or_region	denotes	satellite
T7843	19153-19166	GO:0097617	denotes	hybridization
T7844	19178-19187	PR_EXT:000005402	denotes	p150CAF-1
T7845	19197-19202	CL_GO_EXT:cell	denotes	cells
T7846	19252-19279	GO:0005721	denotes	pericentric heterochromatin
T7847	19367-19372	CL_GO_EXT:cell	denotes	cells
T7848	19416-19425	PR_EXT:000005402	denotes	p150CAF-1
T7849	19495-19504	PR_EXT:000005402	denotes	p150CAF-1
T7850	19563-19590	GO:0005721	denotes	pericentric heterochromatin
T7851	21346-21351	GO:0033186	denotes	CAF-1
T7852	21373-21380	CHEBI:15358	denotes	histone
T7853	21373-21380	_FRAGMENT	denotes	histone
T7854	21390-21392	PR_EXT:000008603	denotes	H4
T7855	21409-21412	CHEBI_SO_EXT:DNA	denotes	DNA
T7856	21409-21422	GO:0071897	denotes	DNA synthesis
T7857	21480-21489	GO:0000785	denotes	chromatin
T7858	21529-21540	GO:0000786	denotes	nucleosomal
T7859	21561-21568	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T7860	21585-21593	GO_EXT:0004518	denotes	nuclease
T7861	21678-21687	PR_EXT:000005402	denotes	p150CAF-1
T7862	21697-21705	CL:0002322	denotes	ES cells
T7863	21700-21705	CL_GO_EXT:cell	denotes	cells
T7864	21727-21733	SO_EXT:0001026	denotes	genome
T7865	21734-21743	GO:0000785	denotes	chromatin
T7866	21762-21769	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7867	21848-21855	CHEBI:15358	denotes	histone
T7868	21848-21858	PR_EXT:000027594	denotes	histone H3
T7869	21864-21883	GO:0000790	denotes	chromatin in nuclei
T7870	21898-21903	CL_GO_EXT:cell	denotes	cells
T7871	21904-21915	GO:0009294	denotes	transfected
T7872	21933-21942	PR_EXT:000005402	denotes	p150CAF-1
T7873	21943-21947	GO:0016246	denotes	RNAi
T7874	21948-21963	SO_EXT:0000755	denotes	plasmid vectors
T7875	21965-21971	GO:0005634	denotes	Nuclei
T7876	21990-21997	CHEBI_CHMO_EXT:buffer_solution	denotes	buffers
T7877	22013-22017	CHEBI:24866	denotes	salt
T7878	22053-22057	CHEBI:26710	denotes	NaCl
T7879	22065-22069	CHEBI:26710	denotes	NaCl
T7880	22104-22111	CHEBI:15358	denotes	histone
T7881	22104-22114	PR_EXT:000027594	denotes	histone H3
T7882	22141-22150	GO:0000785	denotes	chromatin
T7883	22188-22197	PR_EXT:000005402	denotes	p150CAF-1
T7884	22207-22215	CL:0002322	denotes	ES cells
T7885	22210-22215	CL_GO_EXT:cell	denotes	cells
T7886	22292-22319	GO:0005721	denotes	pericentric heterochromatin
T7887	22376-22387	GO:0000786	denotes	nucleosomal
T7888	22970-22977	GO:0005634	denotes	nuclear
T7889	22978-22990	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T7890	22994-22998	PR_EXT:000005086	denotes	HP1α
T7891	23022-23028	PR_EXT:000005402	denotes	Chaf1a
T7892	23029-23030	SO_EXT:sequence_nullness_or_absence	denotes	−
T7893	23031-23032	SO_EXT:sequence_nullness_or_absence	denotes	−
T7894	23033-23040	UBERON:0000922	denotes	embryos
T7895	23045-23054	PR_EXT:000005402	denotes	p150CAF-1
T7896	23064-23072	CL:0002322	denotes	ES cells
T7897	23067-23072	CL_GO_EXT:cell	denotes	cells
T7898	23116-23132	SO_EXT:epigenetic_modification_entity	denotes	epigenetic marks
T7899	23127-23132	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marks
T7900	23166-23193	GO:0005721	denotes	pericentric heterochromatin
T7901	23211-23214	CHEBI_SO_EXT:DNA	denotes	DNA
T7902	23211-23230	GO:0010424	denotes	DNA CpG methylation
T7903	23219-23230	GO_MOP_EXT:methylation	denotes	methylation
T7904	23245-23262	SO_EXT:satellite_unit_or_region	denotes	satellite repeats
T7905	23319-23328	PR_EXT:000005402	denotes	p150CAF-1
T7906	23338-23343	CL_GO_EXT:cell	denotes	cells
T7907	23368-23375	CL:0002322	denotes	ES cell
T7908	23371-23375	CL_GO_EXT:cell	denotes	cell
T7909	23401-23405	GO:0016246	denotes	RNAi
T7910	23423-23429	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	female
T7911	23435-23439	CL_GO_EXT:cell	denotes	cell
T7912	23446-23449	CHEBI_SO_EXT:DNA	denotes	DNA
T7913	23446-23461	GO:0006306	denotes	DNA methylation
T7914	23450-23461	GO_MOP_EXT:methylation	denotes	methylation
T7915	23491-23496	CL_GO_EXT:cell	denotes	cells
T7916	23540-23551	GO_MOP_EXT:methylation	denotes	methylation
T7917	23567-23574	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7918	23618-23633	GO:0000792	denotes	heterochromatin
T7919	23645-23654	PR_EXT:000005402	denotes	p150CAF-1
T7920	23664-23672	CL:0002322	denotes	ES cells
T7921	23667-23672	CL_GO_EXT:cell	denotes	cells
T7922	23688-23692	GO:0016246	denotes	RNAi
T7923	23693-23707	SO_EXT:0000755	denotes	plasmid vector
T7924	23716-23723	CL:0002322	denotes	ES cell
T7925	23719-23723	CL_GO_EXT:cell	denotes	cell
T7926	23757-23766	PR_EXT:000005402	denotes	p150CAF-1
T7927	23790-23805	GO:0000792	denotes	heterochromatin
T7928	23850-23853	CHEBI_SO_EXT:DNA	denotes	DNA
T7929	23850-23865	GO:0006306	denotes	DNA methylation
T7930	23854-23865	GO_MOP_EXT:methylation	denotes	methylation
T7931	23881-23888	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7932	23935-23942	PR_EXT:000043452	denotes	histone
T7933	23935-23942	CHEBI:15358	denotes	histone
T7934	23935-23956	GO:0016570	denotes	histone modifications
T7935	23943-23956	SO_EXT:sequence_alteration_entity_or_process	denotes	modifications
T7936	23965-23974	GO:0000785	denotes	chromatin
T7937	24027-24036	GO:0000785	denotes	chromatin
T7938	24069-24072	CHEBI_SO_EXT:DNA	denotes	DNA
T7939	24149-24159	GO:0042571	denotes	antibodies
T7940	24170-24179	PR_EXT:000005402	denotes	p150CAF-1
T7941	24239-24256	SO_EXT:satellite_unit_or_region	denotes	satellite repeats
T7942	24332-24341	PR_EXT:000005402	denotes	p150CAF-1
T7943	24378-24405	GO:0005721	denotes	pericentric heterochromatin
T7944	24560-24571	GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation	denotes	centromeric
T7945	24572-24580	SO_EXT:sequence_repeat_unit_or_region	denotes	repeated
T7946	24581-24584	CHEBI_SO_EXT:DNA	denotes	DNA
T7947	24649-24658	PR_EXT:000005402	denotes	p150CAF-1
T7948	24703-24730	GO:0005721	denotes	pericentric heterochromatin
T7949	24771-24778	SO_EXT:0001707	denotes	H3K9me3
T7950	24779-24783	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	mark
T7951	24823-24832	PR_EXT:000005402	denotes	p150CAF-1
T7952	24842-24847	CL_GO_EXT:cell	denotes	cells
T7953	26658-26667	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7954	26668-26673	CL_GO_EXT:cell	denotes	cells
T7955	26696-26703	SO_EXT:0001707	denotes	H3K9me3
T7956	26740-26744	CHEBI:51231	denotes	DAPI
T7957	26807-26814	SO_EXT:0001707	denotes	H3K9me3
T7958	26841-26850	PR_EXT:000005402	denotes	p150CAF-1
T7959	26860-26865	CL_GO_EXT:cell	denotes	cells
T7960	26907-26911	PR_EXT:000005086	denotes	HP1α
T7961	27038-27043	CL_GO_EXT:cell	denotes	cells
T7962	27149-27157	CHEBI_SO_EXT:epitope	denotes	epitopes
T7963	27188-27197	GO_EXT:biological_movement_or_translocation_process	denotes	relocated
T7964	27198-27202	CHEBI:51231	denotes	DAPI
T7965	27266-27271	GO:0033186	denotes	CAF-1
T7966	27275-27283	CL:0002322	denotes	ES cells
T7967	27278-27283	CL_GO_EXT:cell	denotes	cells
T7968	27322-27337	GO:0000792	denotes	heterochromatin
T7969	27376-27394	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic marking
T7970	27387-27394	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	marking
T12865	27439-27448	PR_EXT:000005402	denotes	p150CAF-1
T12866	27479-27484	NCBITaxon:10088	denotes	mouse
T12867	27491-27504	GO:0009790	denotes	embryogenesis
T12868	27512-27520	CL:0002322	denotes	ES cells
T12869	27515-27520	CL_GO_EXT:cell	denotes	cells
T12870	27551-27556	GO:0033186	denotes	CAF-1
T12871	27593-27596	CHEBI_SO_EXT:DNA	denotes	DNA
T12872	27593-27608	GO:0006260	denotes	DNA replication
T12873	27612-27623	NCBITaxon:7742	denotes	vertebrates
T12874	27628-27642	NCBITaxon:8355	denotes	Xenopus laevis
T12875	27644-27653	PR_EXT:000005402	denotes	p150CAF-1
T12876	27689-27693	CL_GO_EXT:cell	denotes	cell
T12877	27689-27703	GO:0051301	denotes	cell divisions
T12878	27757-27762	GO:0033186	denotes	CAF-1
T12879	27779-27788	NCBITaxon:40674	denotes	mammalian
T12880	27789-27793	CL_GO_EXT:cell	denotes	cell
T12881	27823-27826	CHEBI_SO_EXT:DNA	denotes	DNA
T12882	27823-27838	GO:0006260	denotes	DNA replication
T12883	27885-27890	GO:0033186	denotes	CAF-1
T12884	27898-27905	GO:0005634	denotes	nuclear
T12885	27922-27937	GO:0000792	denotes	heterochromatin
T12886	27978-27986	CL:0002322	denotes	ES cells
T12887	27981-27986	CL_GO_EXT:cell	denotes	cells
T12888	28004-28013	PR_EXT:000005402	denotes	p150CAF-1
T12889	28023-28031	CL:0002322	denotes	ES cells
T12890	28026-28031	CL_GO_EXT:cell	denotes	cells
T12891	28039-28042	CHEBI_SO_EXT:DNA	denotes	DNA
T12892	28039-28054	GO:0006260	denotes	DNA replication
T12893	28110-28121	GO:0000786	denotes	nucleosomal
T12894	28143-28158	GO:0000792	denotes	heterochromatin
T12895	28205-28214	PR_EXT:000005402	denotes	p150CAF-1
T12896	28230-28233	PR_EXT:000026474	denotes	PML
T12897	28230-28240	GO:0016605	denotes	PML bodies
T12898	28244-28251	CL:0002322	denotes	ES cell
T12899	28247-28258	GO:0005634	denotes	cell nuclei
T12900	28273-28280	GO:0005634	denotes	nuclear
T12901	28332-28341	PR_EXT:000005402	denotes	p150CAF-1
T12902	28377-28384	CL:0002322	denotes	ES cell
T12903	28380-28384	CL_GO_EXT:cell	denotes	cell
T12904	28380-28393	GO:0051301	denotes	cell division
T12905	28398-28402	CL_GO_EXT:cell	denotes	cell
T12906	28398-28408	GO:0008219	denotes	cell death
T12907	28446-28461	GO:0000792	denotes	heterochromatin
T12908	28561-28568	UBERON:0000922	denotes	embryos
T12909	28573-28578	GO:0016265	denotes	death
T12910	28582-28590	CL:0002322	denotes	ES cells
T12911	28585-28590	CL_GO_EXT:cell	denotes	cells
T12912	28604-28609	GO:0033186	denotes	CAF-1
T12913	28617-28624	GO:0005634	denotes	nuclear
T12914	28641-28656	GO:0000792	denotes	heterochromatin
T12915	28674-28686	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T12916	28768-28773	GO:0033186	denotes	CAF-1
T12917	28812-28817	CL_GO_EXT:cell	denotes	cells
T12918	28822-28827	NCBITaxon:9606	denotes	human
T12919	28828-28832	CL_GO_EXT:cell	denotes	cell
T12920	28874-28885	_FRAGMENT	denotes	pluripotent
T12921	28896-28901	CL:0002248	denotes	cells
T12922	28886-28895	UBERON:0000922	denotes	embryonic
T12923	28886-28901	CL:0002321	denotes	embryonic cells
T12924	28896-28901	CL_GO_EXT:cell	denotes	cells
T12925	28918-28931	UBERON:0019248	denotes	early embryos
T12926	28936-28944	CL:0002322	denotes	ES cells
T12927	28939-28944	CL_GO_EXT:cell	denotes	cells
T12928	28952-28958	SO_EXT:0001026	denotes	genome
T12929	28980-28993	CL:0002371	denotes	somatic cells
T12930	28988-28993	CL_GO_EXT:cell	denotes	cells
T12931	29071-29076	GO:0033186	denotes	CAF-1
T12932	29080-29095	GO:0000792	denotes	heterochromatin
T12933	29128-29136	CL:0002322	denotes	ES cells
T12934	29131-29136	CL_GO_EXT:cell	denotes	cells
T12935	29158-29167	GO:0000785	denotes	chromatin
T12936	29168-29176	CHEBI_PR_EXT:protein	denotes	proteins
T12937	29198-29202	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bind
T12938	29225-29234	GO:0000785	denotes	chromatin
T12939	29249-29257	CL_GO_EXT:cell	denotes	cellular
T12940	29297-29312	GO:0000792	denotes	heterochromatin
T12941	29313-29320	GO:0005634	denotes	nuclear
T12942	29344-29349	GO:0033186	denotes	CAF-1
T12943	29378-29392	GO_PATO_RO_EXT:developmental_differentiation_process_or_quality	denotes	differentiated
T12944	29393-29398	CL_GO_EXT:cell	denotes	cells
T12945	29449-29455	SO_EXT:0001026	denotes	genome
T12946	29498-29506	CL:0002322	denotes	ES cells
T12947	29501-29506	CL_GO_EXT:cell	denotes	cells
T12948	29539-29544	GO:0033186	denotes	CAF-1
T12949	29583-29588	GO:0033186	denotes	CAF-1
T12950	29592-29603	_FRAGMENT	denotes	pluripotent
T12951	29614-29619	CL:0002248	denotes	cells
T12952	29604-29613	UBERON:0000922	denotes	embryonic
T12953	29604-29619	CL:0002321	denotes	embryonic cells
T12954	29614-29619	CL_GO_EXT:cell	denotes	cells
T12955	29639-29648	SO_EXT:biological_conservation_process_or_quality	denotes	conserved
T12956	29678-29698	NCBITaxon:3702	denotes	Arabidopsis thaliana
T12957	29709-29714	GO:0033186	denotes	CAF-1
T12958	29760-29765	GO:0033186	denotes	CAF-1
T12959	29806-29818	GO:0010369	denotes	chromocenter
T12960	29879-29884	GO:0033186	denotes	CAF-1
T12961	29905-29920	GO:0000792	denotes	heterochromatin
T12962	29937-29942	_FRAGMENT	denotes	early
T12963	29949-29956	UBERON:0019248	denotes	embryos
T12964	29943-29948	NCBITaxon:10088	denotes	mouse
T12965	29961-29966	NCBITaxon:10088	denotes	mouse
T12966	29967-29975	CL:0002322	denotes	ES cells
T12967	29970-29975	CL_GO_EXT:cell	denotes	cells
T12968	29990-29995	GO:0033186	denotes	CAF-1
T12969	30059-30066	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T12970	30103-30111	SO_EXT:sequence_repeat_unit_or_region	denotes	repeated
T12971	30112-30121	SO_EXT:biological_sequence	denotes	sequences
T12972	30142-30147	NCBITaxon:10088	denotes	mouse
T12973	30148-30154	SO_EXT:0001026	denotes	genome
T12974	30187-30194	GO:0005634	denotes	nuclear
T12975	30212-30230	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic marking
T12976	30223-30230	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	marking
T12977	30234-30261	GO:0005721	denotes	pericentric heterochromatin
T12978	30277-30285	CL:0002322	denotes	ES cells
T12979	30280-30285	CL_GO_EXT:cell	denotes	cells
T12980	30322-30337	GO:0000792	denotes	heterochromatin
T12981	30342-30360	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic marking
T12982	30353-30360	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	marking
T12983	30406-30411	GO:0033186	denotes	CAF-1
T12984	30442-30447	GO:0033186	denotes	CAF-1
T12985	30455-30462	NCBITaxon:species	denotes	species
T12986	30537-30544	CHEBI:15358	denotes	histone
T12987	30537-30547	PR_EXT:000027594	denotes	histone H3
T12988	30548-30556	SO_EXT:sequence_variant_entity	denotes	variants
T12989	30577-30587	NCBITaxon:2759	denotes	eukaryotes
T12990	30589-30594	GO:0033186	denotes	CAF-1
T12991	30611-30618	NCBITaxon:40674	denotes	mammals
T12992	30638-30662	NCBITaxon:4932	denotes	Saccharomyces cerevisiae
T12993	30688-30695	GO:0007114	denotes	budding
T12994	30688-30701	NCBITaxon:4892	denotes	budding yeast
T12995	30702-30708	SO_EXT:0001026	denotes	genome
T12996	30726-30741	GO:0000792	denotes	heterochromatin
T12997	30780-30790	NCBITaxon:2759	denotes	eukaryotes
T12998	30825-30840	GO:0000792	denotes	heterochromatin
T12999	30849-30852	_FRAGMENT	denotes	di-
T13000	30860-30879	GO:0036123	denotes	methylation at H3K9
T13001	30860-30874	_FRAGMENT	denotes	methylation at
T13002	30892-30897	GO:0034772	denotes	H4K20
T13003	30857-30879	GO:0036124	denotes	trimethylation at H3K9
T13004	30857-30874	_FRAGMENT	denotes	trimethylation at
T13005	30881-30886	GO:0070734	denotes	H3K27
T13006	30892-30897	GO:0034773	denotes	H4K20
T13007	30860-30871	GO_MOP_EXT:methylation	denotes	methylation
T13008	30922-30938	GO:0035328	denotes	silent chromatin
T13009	30942-30955	NCBITaxon:4932	denotes	S. cerevisiae
T13010	30960-30967	NCBITaxon:40674	denotes	mammals
T13011	30969-30987	GO:0036123	denotes	H3K9 dimethylation
T13012	30976-30987	GO_MOP_EXT:methylation	denotes	methylation
T13013	31040-31044	PR_EXT:000008425	denotes	H3.3
T13014	31060-31073	SO_EXT:sequence_alteration_entity_or_process	denotes	modifications
T13015	31093-31104	GO_EXT:transcription	denotes	transcribed
T13016	31105-31114	GO:0000785	denotes	chromatin
T13017	33635-33646	GO_MOP_EXT:methylation	denotes	methylation
T13018	31144-31155	GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation	denotes	centromeric
T13019	31156-31158	PR_EXT:000027594	denotes	H3
T13020	31159-31166	SO_EXT:sequence_variant_entity	denotes	variant
T13021	31168-31181	NCBITaxon:4932	denotes	S. cerevisiae
T13022	31197-31201	PR_EXT:000008425	denotes	H3.3
T13023	31212-31221	GO:0010467	denotes	expressed
T13024	31244-31253	GO:0000785	denotes	chromatin
T13025	31259-31270	GO:0006260	denotes	replication
T13026	31301-31311	NCBITaxon:2759	denotes	eukaryotes
T13027	31365-31380	GO:0000792	denotes	heterochromatin
T13028	31408-31413	GO:0033186	denotes	CAF-1
T13029	31431-31442	GO:0006260	denotes	replication
T13030	31461-31468	GO:0032991	denotes	complex
T13031	31508-31515	GO:0005634	denotes	nuclear
T13032	31532-31559	GO:0005721	denotes	pericentric heterochromatin
T13033	31563-31570	UBERON:0000922	denotes	embryos
T13034	31575-31583	CL:0002322	denotes	ES cells
T13035	31578-31583	CL_GO_EXT:cell	denotes	cells
T13036	31657-31666	GO:0000785	denotes	chromatin
T13037	31694-31701	GO:0005634	denotes	nuclear
T13038	31731-31746	GO:0000792	denotes	heterochromatin
T13039	31753-31762	UBERON:0000922	denotes	embryonic
T13040	31916-31931	GO:0000792	denotes	heterochromatin
T13041	31943-31952	PR_EXT:000005402	denotes	p150CAF-1
T13042	31953-31957	SO_EXT:sequence_nullness	denotes	null
T13043	31958-31965	UBERON:0000922	denotes	embryos
T13044	31970-31978	CL:0002322	denotes	ES cells
T13045	31973-31978	CL_GO_EXT:cell	denotes	cells
T13046	31980-31985	GO:0033186	denotes	CAF-1
T13047	32026-32041	GO:0000792	denotes	heterochromatin
T13048	32152-32155	CHEBI_SO_EXT:DNA	denotes	DNA
T13049	32152-32167	GO:0006260	denotes	DNA replication
T13050	32186-32195	PR_EXT:000005402	denotes	p150CAF-1
T13051	32197-32212	GO:0000792	denotes	heterochromatin
T13052	32243-32251	CHEBI:36357	denotes	molecule
T13053	32344-32351	CHEBI_PR_EXT:protein	denotes	protein
T13054	32374-32379	GO:0033186	denotes	CAF-1
T13055	32445-32460	GO:0000792	denotes	heterochromatin
T13056	32468-32480	GO:0007566	denotes	implantation
T13057	32481-32488	UBERON:0000922	denotes	embryos
T13058	32521-32528	GO:0005634	denotes	nuclear
T13059	32554-32562	_FRAGMENT	denotes	two-cell
T13060	32578-32584	UBERON:0007232	denotes	stages
T13061	32558-32562	CL_GO_EXT:cell	denotes	cell
T13062	32567-32577	UBERON:0000358	denotes	blastocyst
T13063	32586-32593	SO_EXT:sequence_cloning_process	denotes	Cloning
T13064	32609-32614	NCBITaxon:10088	denotes	mouse
T13065	32633-32640	GO:0005634	denotes	nuclear
T13066	32657-32675	_FRAGMENT	denotes	heterochromatin in
T13067	32684-32690	GO:0005720	denotes	nuclei
T13068	32676-32683	CL:0002322	denotes	ES cell
T13069	32679-32690	GO:0005634	denotes	cell nuclei
T13070	32721-32735	UBERON:0000106	denotes	one-cell stage
T13071	32725-32729	CL_GO_EXT:cell	denotes	cell
T13072	32756-32763	GO:0005634	denotes	nuclear
T13073	32764-32772	GO_EXT:biological_movement_or_translocation_process	denotes	transfer
T13074	32800-32815	GO:0000792	denotes	heterochromatin
T13075	32843-32850	GO:0005634	denotes	nuclear
T13076	32875-32890	UBERON:0019248	denotes	early embryonic
T13077	32901-32907	SO_EXT:sequence_cloning_process	denotes	cloned
T13078	32908-32915	UBERON:0000922	denotes	embryos
T13079	32948-32963	GO:0000792	denotes	heterochromatin
T13080	33008-33017	PR_EXT:000005402	denotes	p150CAF-1
T13081	33027-33035	CL:0002322	denotes	ES cells
T13082	33030-33035	CL_GO_EXT:cell	denotes	cells
T13083	33072-33076	CL_GO_EXT:cell	denotes	cell
T13084	33083-33089	PR_EXT:000005402	denotes	Chaf1a
T13085	33089-33090	SO_EXT:sequence_nullness_or_absence	denotes	−
T13086	33091-33092	SO_EXT:sequence_nullness_or_absence	denotes	−
T13087	33093-33100	UBERON:0000922	denotes	embryos
T13088	33123-33128	GO:0033186	denotes	CAF-1
T13089	33162-33177	GO:0000792	denotes	heterochromatin
T13090	33208-33212	CL_GO_EXT:cell	denotes	cell
T13091	33208-33222	GO:0051301	denotes	cell divisions
T13092	33226-33240	UBERON_EXT:embryonic_stage_or_stage_part	denotes	embryonic life
T13093	33266-33273	GO:0005634	denotes	nuclear
T13094	33294-33320	GO:0010468	denotes	control of gene expression
T13095	33305-33309	SO_EXT:0000704	denotes	gene
T13096	33357-33362	GO:0033186	denotes	CAF-1
T13097	33406-33410	SO_EXT:0000704	denotes	gene
T13098	33406-33421	GO:0010467	denotes	gene expression
T13099	33435-33448	GO:0009790	denotes	embryogenesis
T13100	33509-33518	GO:0000785	denotes	chromatin
T13101	33560-33571	_FRAGMENT	denotes	pluripotent
T13102	33582-33587	CL:0002248	denotes	cells
T13103	33572-33581	UBERON:0000922	denotes	embryonic
T13104	33572-33587	CL:0002321	denotes	embryonic cells
T13105	33582-33587	CL_GO_EXT:cell	denotes	cells
T13106	33631-33634	CHEBI_SO_EXT:DNA	denotes	DNA
T13107	33631-33646	GO:0006306	denotes	DNA methylation
T13108	33651-33658	PR_EXT:000043452	denotes	histone
T13109	33651-33658	CHEBI:15358	denotes	histone
T13110	33651-33668	GO:0016570	denotes	histone modifying
T13111	33659-33668	SO_EXT:sequence_alteration_process	denotes	modifying
T13112	33669-33676	CHEBI_GO_SO_EXT:enzyme	denotes	enzymes
T13113	33722-33731	GO:0000785	denotes	chromatin
T13114	33722-33740	GO:0031497	denotes	chromatin assembly
T13115	33791-33802	GO:0000786	denotes	nucleosomes
T13116	33804-33809	GO:0033186	denotes	CAF-1
T13117	33831-33841	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic
T13118	33857-33872	GO:0000792	denotes	heterochromatin
T13119	33884-33897	UBERON:0019248	denotes	early embryos
T13120	33902-33910	CL:0002322	denotes	ES cells
T13121	33905-33910	CL_GO_EXT:cell	denotes	cells
T14773	33936-33949	_FRAGMENT	denotes	Generation of
T14774	33973-33980	GO:0009790	denotes	embryos
T14775	33950-33956	PR_EXT:000005402	denotes	Chaf1a
T14776	33957-33963	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T14777	33964-33968	NCBITaxon:10088	denotes	mice
T14778	33973-33980	UBERON:0000922	denotes	embryos
T14779	34010-34017	SO_EXT:0001026	denotes	genomic
T14780	34038-34039	CHEBI_SO_EXT:base	denotes	b
T14781	34046-34054	SO:0000357	denotes	flanking
T14782	34055-34061	PR_EXT:000005402	denotes	Chaf1a
T14783	34062-34066	SO_EXT:0000147	denotes	exon
T14784	34076-34079	CHEBI_SO_EXT:DNA	denotes	DNA
T14785	34121-34128	SO_EXT:sequence_cloning_process	denotes	cloning
T14786	34138-34146	CHEBI:7507	denotes	neomycin
T14787	34166-34171	CHEBI_EXT:27026	denotes	toxin
T14788	34173-34182	SO_EXT:0005853	denotes	cassettes
T14789	34215-34224	SO_EXT:engineered_biological_sequence	denotes	construct
T14790	34229-34240	GO:0009294	denotes	transfected
T14791	34244-34252	CL:0002322	denotes	ES cells
T14792	34247-34252	CL_GO_EXT:cell	denotes	cells
T14793	34287-34298	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T14794	34299-34307	CL:0002322	denotes	ES cells
T14795	34302-34307	CL_GO_EXT:cell	denotes	cells
T14796	34321-34327	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T14797	34328-34334	PR_EXT:000005402	denotes	Chaf1a
T14798	34355-34361	PR_EXT:000005402	denotes	Chaf1a
T14799	34383-34389	SO_EXT:0001023	denotes	allele
T14800	34431-34437	PR_EXT:000005402	denotes	Chaf1a
T14801	34438-34439	SO_EXT:normal_or_wild_type_or_present	denotes	+
T14802	34440-34441	SO_EXT:sequence_nullness_or_absence	denotes	−
T14803	34442-34446	NCBITaxon:10088	denotes	mice
T14804	34460-34470	GO_SO_EXT:sequence_rearrangement_process	denotes	recombined
T14805	34471-34479	CL:0002322	denotes	ES cells
T14806	34474-34479	CL_GO_EXT:cell	denotes	cells
T14807	34494-34505	UBERON:0000358	denotes	blastocysts
T14808	34510-34517	UBERON:0000922	denotes	embryos
T14809	34550-34556	PR_EXT:000005402	denotes	Chaf1a
T14810	34557-34558	SO_EXT:normal_or_wild_type_or_present	denotes	+
T14811	34559-34560	SO_EXT:sequence_nullness_or_absence	denotes	−
T14812	34561-34565	NCBITaxon:10088	denotes	mice
T14813	34610-34617	UBERON:0000922	denotes	Embryos
T14814	34651-34654	CHEBI:60004	denotes	mix
T14815	34666-34681	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T14816	34682-34689	SO_EXT:0000112	denotes	primers
T14817	34731-34740	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T14818	34745-34751	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T14819	34752-34759	SO_EXT:0001023	denotes	alleles
T14820	34887-34902	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T14821	34903-34910	SO_EXT:0000112	denotes	primers
T14822	34977-34984	CHEBI:2511	denotes	agarose
T14823	35025-35034	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T14824	35040-35042	SO_EXT:0000028	denotes	bp
T14825	35048-35059	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T14826	35065-35067	SO_EXT:0000028	denotes	bp
T14827	35069-35076	SO_EXT:0001023	denotes	alleles
T15643	35079-35088	PR_EXT:000005402	denotes	P150CAF-1
T15644	35102-35106	GO:0016246	denotes	RNAi
T15645	35112-35116	GO:0016246	denotes	RNAi
T15646	35117-35131	SO_EXT:0000755	denotes	plasmid vector
T15647	35172-35177	NCBITaxon:10088	denotes	mouse
T15648	35181-35189	SO_EXT:0000167	denotes	promoter
T15649	35196-35205	CHEBI:17939	denotes	puromycin
T15650	35216-35220	SO_EXT:0000704	denotes	gene
T15651	35306-35312	SO_EXT:0000440	denotes	vector
T15652	35332-35336	SO_EXT:0000704	denotes	gene
T15653	35368-35373	SO_EXT:0000704	denotes	genes
T15654	35377-35385	CL:0002322	denotes	ES cells
T15655	35380-35385	CL_GO_EXT:cell	denotes	cells
T15656	35420-35425	CL_GO_EXT:cell	denotes	cells
T15657	35502-35507	CL_GO_EXT:cell	denotes	cells
T15658	35522-35530	SO_EXT:biological_sequence	denotes	sequence
T15659	35534-35543	PR_EXT:000005402	denotes	p150CAF-1
T15660	35544-35549	SO_EXT:0000646	denotes	siRNA
T15661	35551-35572	SO_EXT:0000646	denotes	short interfering RNA
T15662	35569-35572	CHEBI_SO_EXT:RNA	denotes	RNA
T15663	35590-35596	SO_EXT:sequence_cloning_process	denotes	cloned
T15664	35607-35614	SO_EXT:0000155	denotes	plasmid
T15665	35628-35634	SO_EXT:0000440	denotes	vector
T15666	35635-35644	GO:0010467	denotes	expressed
T15667	35647-35652	SO_EXT:0000646	denotes	siRNA
T15668	35670-35673	CHEBI_SO_EXT:RNA	denotes	RNA
T15669	35670-35685	GO:0006401	denotes	RNA degradation
T15670	35687-35695	CL:0002322	denotes	ES cells
T15671	35690-35695	CL_GO_EXT:cell	denotes	cells
T15672	35701-35712	GO:0009294	denotes	transfected
T15673	35741-35744	CHEBI_SO_EXT:RNA	denotes	RNA
T15674	35745-35759	SO_EXT:0000755	denotes	plasmid vector
T15675	35773-35780	CHEBI:5291	denotes	gelatin
T15676	35847-35856	CHEBI:17939	denotes	Puromycin
T15677	35903-35908	CL_GO_EXT:cell	denotes	cells
T15678	35964-35973	PR_EXT:000005402	denotes	p150CAF-1
T15679	35974-35978	GO:0016246	denotes	RNAi
T15680	35979-35985	SO_EXT:0000440	denotes	vector
T15681	36035-36040	GO:0033186	denotes	CAF-1
T15682	36059-36064	CL_GO_EXT:cell	denotes	cells
T15683	36131-36140	PR_EXT:000005402	denotes	p150CAF-1
T15684	36141-36151	GO:0010467	denotes	expression
T15685	36181-36186	CL_GO_EXT:cell	denotes	cells
T15686	36218-36222	SO_EXT:0000704	denotes	gene
T15687	36262-36273	GO:0009294	denotes	transfected
T15688	36294-36298	GO:0016246	denotes	RNAi
T15689	36299-36313	SO_EXT:0000755	denotes	plasmid vector
T15690	36430-36435	CL_GO_EXT:cell	denotes	Cells
T15691	36441-36452	GO_EXT:trypsinization	denotes	trypsinized
T15692	36519-36528	CHEBI:17939	denotes	puromycin
T16159	36531-36535	CL_GO_EXT:cell	denotes	Cell
T16160	36548-36556	CL:0002322	denotes	ES cells
T16161	36551-36556	CL_GO_EXT:cell	denotes	cells
T16162	36598-36602	SO_EXT:0000704	denotes	gene
T16163	36619-36627	CL:0002322	denotes	ES cells
T16164	36622-36627	CL_GO_EXT:cell	denotes	cells
T16165	36645-36649	GO:0016246	denotes	RNAi
T16166	36679-36683	CL_GO_EXT:cell	denotes	cell
T16167	36713-36720	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T16168	36721-36725	GO_EXT:breeding	denotes	bred
T16169	36739-36744	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T16170	36758-36766	CL:0002322	denotes	ES cells
T16171	36761-36766	CL_GO_EXT:cell	denotes	cells
T16172	36841-36848	UBERON:0000922	denotes	embryos
T16568	36892-36897	CL_GO_EXT:cell	denotes	Cells
T16569	36935-36951	CHEBI_EXT:paraformaldehye	denotes	paraformaldehyde
T16570	37020-37027	UBERON:0000922	denotes	embryos
T16571	37067-37071	CHEBI_EXT:37527	denotes	acid
T16572	37086-37100	UBERON:0000086	denotes	zona pellucida
T16573	37186-37194	CL:0002322	denotes	ES cells
T16574	37189-37194	CL_GO_EXT:cell	denotes	cells
T16575	37196-37206	GO:0042571	denotes	Antibodies
T16576	37212-37216	PR_EXT:000005086	denotes	HP1α
T16577	37301-37305	CHEBI:472552	denotes	BrdU
T16578	37348-37352	PR_EXT:000012421	denotes	PCNA
T16579	37380-37383	PR_EXT:000026474	denotes	PML
T16580	37480-37490	GO:0042571	denotes	Antibodies
T16581	37496-37501	NCBITaxon:10088	denotes	mouse
T16582	37502-37510	PR_EXT:000005402	denotes	p150CAF1
T16583	37525-37532	SO_EXT:0001707	denotes	H3K9me3
T16584	37615-37625	GO:0042571	denotes	antibodies
T16585	37646-37655	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T16586	37656-37662	CHEBI_SO_EXT:molecular_probe	denotes	Probes
T16913	37727-37732	CL_GO_EXT:cell	denotes	Cells
T16914	37738-37743	GO:0019835	denotes	lysed
T16915	37754-37760	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T16916	37783-37786	CHEBI:8984	denotes	SDS
T16917	37814-37824	GO:0042571	denotes	antibodies
T16918	37833-37842	PR_EXT:000005402	denotes	p150CAF-1
T16919	37867-37871	PR_EXT:000005086	denotes	HP1α
T16920	37897-37904	CHEBI:15358	denotes	Histone
T16921	37897-37907	PR_EXT:000027594	denotes	Histone H3
T16922	37924-37931	SO_EXT:0001707	denotes	H3K9me3
T16923	37953-37960	PR_EXT:000003676	denotes	β-actin
T17162	37983-37986	CHEBI_SO_EXT:DNA	denotes	DNA
T17163	37993-37999	CHEBI_SO_EXT:molecular_probe	denotes	Probes
T17164	38032-38038	CHEBI:15956	denotes	Biotin
T17165	38042-38046	CHEBI_EXT:dUTP	denotes	dUTP
T17166	38050-38061	CHEBI:42098	denotes	Digoxigenin
T17167	38065-38069	CHEBI_EXT:dUTP	denotes	dUTP
T17168	38098-38105	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T17169	38106-38112	CHEBI_SO_EXT:molecular_probe	denotes	probes
T17170	38136-38147	GO:0006412	denotes	translation
T17877	38389-38395	GO:0005634	denotes	Nuclei
T17878	38409-38417	GO_EXT:0004518	denotes	nuclease
T17879	38433-38444	CHEBI_EXT:biochemical	denotes	biochemical
T17880	38457-38466	GO:0000785	denotes	chromatin
T17881	38468-38476	CL:0002322	denotes	ES cells
T17882	38471-38476	CL_GO_EXT:cell	denotes	cells
T17883	38513-38519	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T17884	38529-38533	CHEBI:9754	denotes	Tris
T17885	38534-38537	CHEBI:17883	denotes	HCl
T17886	38554-38561	CHEBI:17992	denotes	sucrose
T17887	38569-38572	CHEBI:32588	denotes	KCl
T17888	38580-38584	CHEBI:26710	denotes	NaCl
T17889	38591-38596	CHEBI:6636	denotes	MgCl2
T17890	38605-38609	CHEBI_EXT:EGTA	denotes	EGTA
T17891	38674-38680	GO:0005634	denotes	Nuclei
T17892	38749-38756	CHEBI:17992	denotes	sucrose
T17893	38767-38773	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T17894	38787-38794	CHEBI:17992	denotes	sucrose
T17895	38815-38823	GO_EXT:0004518	denotes	nuclease
T17896	38824-38830	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T17897	38838-38842	CHEBI:9754	denotes	Tris
T17898	38843-38846	CHEBI:17883	denotes	HCl
T17899	38863-38867	CHEBI:26710	denotes	NaCl
T17900	38876-38883	CHEBI:17992	denotes	sucrose
T17901	38890-38895	CHEBI:6636	denotes	MgCl2
T17902	38902-38907	CHEBI:3312	denotes	CaCl2
T17903	38922-38928	GO:0005634	denotes	nuclei
T17904	39000-39007	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T17905	39084-39091	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T17906	39127-39130	CHEBI:8984	denotes	SDS
T17907	39141-39145	CHEBI_EXT:EDTA	denotes	EDTA
T17908	39156-39160	CHEBI_SO_EXT:DNA	denotes	DNAs
T17909	39178-39188	GO_EXT:0008233	denotes	proteinase
T17910	39213-39219	CHEBI:15882	denotes	phenol
T17911	39220-39230	CHEBI:35255	denotes	chloroform
T17912	39246-39257	CHEBI:17824	denotes	isopropanol
T17913	39300-39307	CHEBI:15358	denotes	histone
T17914	39300-39310	PR_EXT:000027594	denotes	histone H3
T17915	39316-39325	GO:0000785	denotes	chromatin
T17916	39341-39345	PR_EXT:000005402	denotes	p150
T17917	39355-39360	CL_GO_EXT:cell	denotes	cells
T17918	39371-39377	GO:0005634	denotes	nuclei
T17919	39414-39420	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T17920	39430-39435	CHEBI:46756	denotes	Hepes
T17921	39449-39457	CHEBI:17754	denotes	Glycerol
T17922	39464-39469	CHEBI:6636	denotes	MgCl2
T17923	39491-39494	CHEBI:18320	denotes	DTT
T17924	39503-39507	CHEBI:8102	denotes	PMSF
T17925	39570-39574	CHEBI:26710	denotes	NaCl
T17926	39671-39678	CHEBI:15358	denotes	histone
T17927	39671-39681	PR_EXT:000027594	denotes	histone H3
T17928	39682-39690	GO:0042571	denotes	antibody
T18609	39726-39735	GO:0000785	denotes	chromatin
T18610	39760-39771	GO:0000786	denotes	nucleosomes
T18611	39809-39818	GO:0000785	denotes	chromatin
T18612	39869-39879	GO:0042571	denotes	antibodies
T18613	39903-39910	CHEBI_PR_EXT:protein	denotes	protein
T18614	39960-39963	CHEBI_SO_EXT:DNA	denotes	DNA
T18615	39992-39999	CHEBI:2511	denotes	agarose
T18616	40036-40049	GO:0097617	denotes	Hybridization
T18617	40151-40161	GO:0042571	denotes	Antibodies
T18618	40211-40221	GO:0042571	denotes	Antibodies
T18619	40226-40233	SO_EXT:0001707	denotes	H3K9me3
T18620	40359-40361	SO_EXT:0000028	denotes	bp
T18621	40368-40373	PR_EXT:P23940	denotes	BamHI
T18622	40400-40409	SO_EXT:satellite_unit_or_region	denotes	satellite
T18623	40410-40415	CHEBI_SO_EXT:molecular_probe	denotes	probe
T18624	40435-40437	SO_EXT:0000028	denotes	bp
T18625	40444-40451	PR_EXT:P43870	denotes	HindIII
T18626	40495-40501	SO_EXT:sequence_copy_entity	denotes	copies
T18627	40513-40515	SO_EXT:0000028	denotes	bp
T18628	40522-40538	SO_EXT:satellite_unit_or_region	denotes	satellite repeat
T18629	40545-40551	CHEBI_SO_EXT:molecular_probe	denotes	Probes
T18630	40608-40613	CHEBI_SO_EXT:molecular_probe	denotes	probe
T7612	10085-10098	CL:0002371	denotes	somatic cells
R792	T7721	T7720	_lexicallyChainedTo	nucleus,periphery of
R793	T7626	T7625	_lexicallyChainedTo	stages,two-cell
R794	T7638	T7637	_lexicallyChainedTo	stages,four-cell
R796	T7649	T7648	_lexicallyChainedTo	stages,two-cell
R797	T7657	T7656	_lexicallyChainedTo	cell stage,eight-
R798	T7664	T7663	_lexicallyChainedTo	nuclei,periphery of
R799	T7677	T7676	_lexicallyChainedTo	cell stage,two-
R800	T7686	T7685	_lexicallyChainedTo	nuclei,heterochromatin within
R801	T7815	T7814	_lexicallyChainedTo	nucleus,heterochromatin in
R802	T7825	T7824	_lexicallyChainedTo	chromosomes,domains from
R803	T7835	T7834	_lexicallyChainedTo	chromosomes,domains from
R804	T7854	T7853	_lexicallyChainedTo	H4,histone
R3069	T12921	T12920	_lexicallyChainedTo	cells,pluripotent
R3070	T12951	T12950	_lexicallyChainedTo	cells,pluripotent
R3071	T12963	T12962	_lexicallyChainedTo	embryos,early
R3072	T13000	T12999	_lexicallyChainedTo	methylation at H3K9,di-
R3073	T13001	T12999	_lexicallyChainedTo	methylation at,di-
R3074	T13002	T13001	_lexicallyChainedTo	H4K20,methylation at
R3075	T13005	T13004	_lexicallyChainedTo	H3K27,trimethylation at
R3076	T13006	T13004	_lexicallyChainedTo	H4K20,trimethylation at
R3077	T13060	T13059	_lexicallyChainedTo	stages,two-cell
R3078	T13067	T13066	_lexicallyChainedTo	nuclei,heterochromatin in
R3079	T13102	T13101	_lexicallyChainedTo	cells,pluripotent
R4194	T14774	T14773	_lexicallyChainedTo	embryos,Generation of
R5	T715	T714	_lexicallyChainedTo	Cells,Pluripotent
R6	T746	T745	_lexicallyChainedTo	stages,two-cell
R7	T756	T755	_lexicallyChainedTo	cell stage,two-
R8	T776	T775	_lexicallyChainedTo	marks,epigenetic
R9	T793	T792	_lexicallyChainedTo	cells,pluripotent
R325	T2211	T2210	_lexicallyChainedTo	H4,histone
R326	T2271	T2270	_lexicallyChainedTo	cells,embryonic stem
R327	T2271	T2272	_lexicallyChainedTo	cells,ES
R328	T2284	T2283	_lexicallyChainedTo	embryos,early
R329	T2293	T2292	_lexicallyChainedTo	cells,pluripotent

2_test

Id	Subject	Object	Predicate	Lexical cue
17083276-11689958-85837791	3448-3449	11689958	denotes	1
17083276-11498580-85837792	3450-3451	11498580	denotes	2
17083276-15809273-85837793	3619-3620	15809273	denotes	3
17083276-14671313-85837793	3619-3620	14671313	denotes	3
17083276-12209141-85837793	3619-3620	12209141	denotes	3
17083276-15996204-85837794	3829-3830	15996204	denotes	6
17083276-15020040-85837795	4073-4074	15020040	denotes	7
17083276-12923523-85837796	4075-4076	12923523	denotes	8
17083276-7600578-85837797	4294-4295	7600578	denotes	9
17083276-8808624-85837798	4296-4298	8808624	denotes	10
17083276-14718166-85837799	4545-4547	14718166	denotes	11
17083276-16212490-85837800	4548-4550	16212490	denotes	12
17083276-10549285-85837801	4852-4854	10549285	denotes	13
17083276-12697822-85837801	4852-4854	12697822	denotes	13
17083276-15327775-85837801	4852-4854	15327775	denotes	13
17083276-15306854-85837802	5027-5029	15306854	denotes	16
17083276-15306854-85837803	10036-10038	15306854	denotes	16
17083276-11336697-85837804	10039-10041	11336697	denotes	17
17083276-14532126-85837804	10039-10041	14532126	denotes	17
17083276-15071554-85837804	10039-10041	15071554	denotes	17
17083276-15302854-85837805	10218-10220	15302854	denotes	20
17083276-15598472-85837806	10438-10440	15598472	denotes	21
17083276-15306854-85837807	13316-13318	15306854	denotes	16
17083276-15077145-85837808	15068-15070	15077145	denotes	22
17083276-15306854-85837809	15769-15771	15306854	denotes	16
17083276-14519857-85837810	15772-15774	14519857	denotes	23
17083276-12620223-85837811	15775-15777	12620223	denotes	24
17083276-15302854-85837812	18189-18191	15302854	denotes	20
17083276-1606614-85837813	19903-19905	1606614	denotes	47
17083276-6694730-85837814	19937-19939	6694730	denotes	48
17083276-14718166-85837815	21424-21426	14718166	denotes	11
17083276-16212490-85837816	21427-21429	16212490	denotes	12
17083276-1606614-85837817	22936-22938	1606614	denotes	47
17083276-11782440-85837818	23232-23234	11782440	denotes	25
17083276-16244654-85837819	23498-23500	16244654	denotes	26
17083276-15702071-85837820	23958-23960	15702071	denotes	27
17083276-15273407-85837821	24038-24040	15273407	denotes	28
17083276-15145825-85837822	24142-24144	15145825	denotes	29
17083276-15128874-85837823	24145-24147	15128874	denotes	30
17083276-15678104-85837824	24621-24623	15678104	denotes	31
17083276-11701123-85837825	24785-24787	11701123	denotes	32
17083276-1606614-85837826	25311-25313	1606614	denotes	47
17083276-1606614-85837827	25821-25823	1606614	denotes	47
17083276-8013255-85837828	25989-25991	8013255	denotes	49
17083276-9771701-85837829	26082-26084	9771701	denotes	50
17083276-15145825-85837830	26782-26784	15145825	denotes	29
17083276-11296234-85837831	27741-27743	11296234	denotes	33
17083276-15306854-85837832	27840-27842	15306854	denotes	16
17083276-14519857-85837833	27843-27845	14519857	denotes	23
17083276-12620223-85837834	27846-27848	12620223	denotes	24
17083276-15024074-85837835	27849-27851	15024074	denotes	34
17083276-16399082-85837836	29236-29238	16399082	denotes	35
17083276-11163246-85837837	29747-29749	11163246	denotes	36
17083276-16452472-85837838	29825-29827	16452472	denotes	37
17083276-9030687-85837839	30664-30666	9030687	denotes	38
17083276-16267050-85837840	31124-31126	16267050	denotes	39
17083276-16212490-85837841	31313-31315	16212490	denotes	12
17083276-14718166-85837842	31470-31472	14718166	denotes	11
17083276-10549285-85837843	32381-32383	10549285	denotes	13
17083276-15306854-85837844	32384-32386	15306854	denotes	16
17083276-16680825-85837845	32424-32426	16680825	denotes	40
17083276-16680825-85837846	32774-32776	16680825	denotes	40
17083276-15598472-85837847	33322-33324	15598472	denotes	21
17083276-15198979-85837848	33325-33327	15198979	denotes	41
17083276-15809273-85837849	33678-33679	15809273	denotes	3
17083276-15306854-85837850	35582-35584	15306854	denotes	16
17083276-12529396-85837851	36558-36560	12529396	denotes	42
17083276-11236671-85837852	36866-36868	11236671	denotes	43
17083276-11175742-85837853	37012-37014	11175742	denotes	44
17083276-15306854-85837854	37512-37514	15306854	denotes	16
17083276-15306854-85837855	37845-37847	15306854	denotes	16
17083276-15302854-85837856	38027-38029	15302854	denotes	20
17083276-15302854-85837857	38189-38191	15302854	denotes	20
17083276-15273407-85837858	39796-39798	15273407	denotes	28
17083276-11593003-85837859	40418-40420	11593003	denotes	45
17083276-12867029-85837859	40418-40420	12867029	denotes	45
17083276-1606614-85837859	40418-40420	1606614	denotes	45
17083276-8013255-85837860	40540-40542	8013255	denotes	49
17083276-12867029-85837861	40596-40598	12867029	denotes	46
17083276-1606614-85837861	40596-40598	1606614	denotes	46
17083276-6694730-85837861	40596-40598	6694730	denotes	46
17083276-9771701-85837862	40649-40651	9771701	denotes	50
T50250	3448-3449	11689958	denotes	1
T78309	3450-3451	11498580	denotes	2
T83513	3619-3620	15809273	denotes	3
T75331	3619-3620	14671313	denotes	3
T60495	3619-3620	12209141	denotes	3
T48022	3829-3830	15996204	denotes	6
T53440	4073-4074	15020040	denotes	7
T31781	4075-4076	12923523	denotes	8
T40521	4294-4295	7600578	denotes	9
T17376	4296-4298	8808624	denotes	10
T52713	4545-4547	14718166	denotes	11
T53499	4548-4550	16212490	denotes	12
T70799	4852-4854	10549285	denotes	13
T50230	4852-4854	12697822	denotes	13
T88647	4852-4854	15327775	denotes	13
T52730	5027-5029	15306854	denotes	16
T85283	10036-10038	15306854	denotes	16
T95548	10039-10041	11336697	denotes	17
T47164	10039-10041	14532126	denotes	17
T89267	10039-10041	15071554	denotes	17
T79104	10218-10220	15302854	denotes	20
T19555	10438-10440	15598472	denotes	21
T8277	13316-13318	15306854	denotes	16
T48667	15068-15070	15077145	denotes	22
T83132	15769-15771	15306854	denotes	16
T39792	15772-15774	14519857	denotes	23
T62977	15775-15777	12620223	denotes	24
T32017	18189-18191	15302854	denotes	20
T91663	19903-19905	1606614	denotes	47
T5011	19937-19939	6694730	denotes	48
T63760	21424-21426	14718166	denotes	11
T93971	21427-21429	16212490	denotes	12
T60830	22936-22938	1606614	denotes	47
T68317	23232-23234	11782440	denotes	25
T4146	23498-23500	16244654	denotes	26
T27479	23958-23960	15702071	denotes	27
T52202	24038-24040	15273407	denotes	28
T4457	24142-24144	15145825	denotes	29
T22200	24145-24147	15128874	denotes	30
T3965	24621-24623	15678104	denotes	31
T41821	24785-24787	11701123	denotes	32
T42866	25311-25313	1606614	denotes	47
T25351	25821-25823	1606614	denotes	47
T15798	25989-25991	8013255	denotes	49
T32061	26082-26084	9771701	denotes	50
T1911	26782-26784	15145825	denotes	29
T24836	27741-27743	11296234	denotes	33
T83175	27840-27842	15306854	denotes	16
T35989	27843-27845	14519857	denotes	23
T18539	27846-27848	12620223	denotes	24
T73645	27849-27851	15024074	denotes	34
T40052	29236-29238	16399082	denotes	35
T41085	29747-29749	11163246	denotes	36
T65299	29825-29827	16452472	denotes	37
T48959	30664-30666	9030687	denotes	38
T40627	31124-31126	16267050	denotes	39
T76754	31313-31315	16212490	denotes	12
T49098	31470-31472	14718166	denotes	11
T44850	32381-32383	10549285	denotes	13
T48971	32384-32386	15306854	denotes	16
T53657	32424-32426	16680825	denotes	40
T4581	32774-32776	16680825	denotes	40
T57577	33322-33324	15598472	denotes	21
T19321	33325-33327	15198979	denotes	41
T72450	33678-33679	15809273	denotes	3
T36041	35582-35584	15306854	denotes	16
T46532	36558-36560	12529396	denotes	42
T26707	36866-36868	11236671	denotes	43
T3704	37012-37014	11175742	denotes	44
T95429	37512-37514	15306854	denotes	16
T63338	37845-37847	15306854	denotes	16
T22708	38027-38029	15302854	denotes	20
T71895	38189-38191	15302854	denotes	20
T79362	39796-39798	15273407	denotes	28
T43192	40418-40420	11593003	denotes	45
T47693	40418-40420	12867029	denotes	45
T79851	40418-40420	1606614	denotes	45
T53573	40540-40542	8013255	denotes	49
T63565	40596-40598	12867029	denotes	46
T20351	40596-40598	1606614	denotes	46
T664	40596-40598	6694730	denotes	46
T4099	40649-40651	9771701	denotes	50

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T645	0-5	GO:0033186	denotes	CAF-1
T646	23-38	GO:0000792	denotes	Heterochromatin
T647	55-66	_FRAGMENT	denotes	Pluripotent
T648	77-82	CL:0002248	denotes	Cells
T649	67-76	UBERON:0000922	denotes	Embryonic
T650	67-82	CL:0002321	denotes	Embryonic Cells
T651	136-145	NCBITaxon:40674	denotes	mammalian
T652	159-168	GO:0000785	denotes	chromatin
T653	219-225	SO:0001026	denotes	genome
T654	287-296	GO:0000785	denotes	chromatin
T655	287-305	GO:0031497	denotes	chromatin assembly
T656	356-374	GO:0031497	denotes	chromatin assembly
T657	356-383	GO:0033186	denotes	chromatin assembly factor 1
T658	385-390	GO:0033186	denotes	CAF-1
T659	403-415	GO:0007566	denotes	implantation
T660	444-449	NCBITaxon:10088	denotes	mouse
T661	491-495	SO:0000704	denotes	gene
T662	524-533	PR:000005402	denotes	p150CAF-1
T663	543-552	PR:000005402	denotes	p150CAF-1
T664	638-647	PR:000005402	denotes	p150CAF-1
T665	657-664	UBERON:0000922	denotes	embryos
T666	702-709	GO:0005634	denotes	nuclear
T667	739-754	GO:0000792	denotes	heterochromatin
T668	794-801	UBERON:0000922	denotes	embryos
T669	803-818	GO:0000792	denotes	heterochromatin
T670	867-875	_FRAGMENT	denotes	two-cell
T671	891-897	UBERON:0007232	denotes	stages
T672	880-890	UBERON:0000358	denotes	blastocyst
T673	902-911	PR:000005402	denotes	p150CAF-1
T674	933-940	UBERON:0000922	denotes	embryos
T675	970-985	GO:0000792	denotes	heterochromatin
T676	1028-1043	GO:0000792	denotes	heterochromatin
T677	1047-1051	_FRAGMENT	denotes	two-
T678	1060-1070	UBERON:0007232	denotes	cell stage
T679	1055-1070	UBERON:0007233	denotes	four-cell stage
T680	1081-1088	UBERON:0000922	denotes	embryos
T681	1106-1111	GO:0033186	denotes	CAF-1
T682	1146-1161	GO:0000792	denotes	heterochromatin
T683	1172-1184	GO:0007566	denotes	implantation
T684	1201-1210	UBERON:0000922	denotes	embryonic
T685	1201-1221	CL:0002322	denotes	embryonic stem cells
T686	1236-1245	PR:000005402	denotes	p150CAF-1
T687	1252-1268	GO:0016246	denotes	RNA interference
T688	1343-1370	GO:0005721	denotes	pericentric heterochromatin
T689	1401-1406	GO:0033186	denotes	CAF-1
T690	1462-1469	PR:000043452	denotes	histone
T691	1462-1469	CHEBI:15358	denotes	histone
T692	1462-1481	GO:0016571	denotes	histone methylation
T693	1504-1531	GO:0005721	denotes	pericentric heterochromatin
T694	1554-1569	GO:0000792	denotes	heterochromatin
T695	1587-1596	PR:000005402	denotes	p150CAF-1
T696	1606-1611	NCBITaxon:10088	denotes	mouse
T697	1612-1621	UBERON:0000922	denotes	embryonic
T698	1622-1633	CL:0000057	denotes	fibroblasts
T699	1703-1708	GO:0033186	denotes	CAF-1
T700	1738-1753	GO:0000792	denotes	heterochromatin
T701	1770-1781	_FRAGMENT	denotes	pluripotent
T702	1792-1797	CL:0002248	denotes	cells
T703	1782-1791	UBERON:0000922	denotes	embryonic
T704	1782-1797	CL:0002321	denotes	embryonic cells
T705	1838-1847	GO:0000785	denotes	chromatin
T706	1838-1856	GO:0031497	denotes	chromatin assembly
T707	1870-1881	GO:0065007	denotes	controlling
T708	1937-1943	SO:0001026	denotes	genome
T709	1947-1960	UBERON:0019248	denotes	early embryos
T710	1965-1974	UBERON:0000922	denotes	embryonic
T711	1965-1985	CL:0002322	denotes	embryonic stem cells
T2060	3244-3249	NCBITaxon:10088	denotes	mouse
T2061	3254-3266	GO:0007566	denotes	implantation
T2062	3284-3290	SO:0001026	denotes	genome
T2063	3351-3360	UBERON:0000922	denotes	embryonic
T2064	3361-3365	SO:0000704	denotes	gene
T2065	3361-3376	GO:0010467	denotes	gene expression
T2066	3508-3523	GO:0006306	denotes	DNA methylation
T2067	3592-3601	GO:0000785	denotes	chromatin
T2068	3667-3674	PR:000043452	denotes	histone
T2069	3667-3674	CHEBI:15358	denotes	histone
T2070	3667-3684	GO:0016570	denotes	histone modifying
T2071	3694-3703	GO:0000785	denotes	chromatin
T2072	3694-3714	GO:0006338	denotes	chromatin remodeling
T2073	3728-3735	PR:000043452	denotes	histone
T2074	3728-3735	CHEBI:15358	denotes	histone
T2075	3758-3770	GO:0007566	denotes	implantation
T2076	3790-3800	CL:0000034	denotes	stem cells
T2077	3814-3827	UBERON:0019248	denotes	early embryos
T2078	3856-3863	PR:000043452	denotes	histone
T2079	3856-3863	CHEBI:15358	denotes	histone
T2080	3879-3888	GO:0000785	denotes	chromatin
T2081	3879-3899	GO:0006338	denotes	chromatin remodeling
T2082	3900-3909	GO:0032991	denotes	complexes
T2083	3937-3947	GO:0000786	denotes	nucleosome
T2084	3937-3956	GO:0006334	denotes	nucleosome assembly
T2085	4011-4018	GO:0065007	denotes	control
T2086	4019-4028	GO:0000785	denotes	chromatin
T2087	4019-4037	GO:0031497	denotes	chromatin assembly
T2088	4085-4092	PR:000043452	denotes	histone
T2089	4085-4092	CHEBI:15358	denotes	histone
T2090	4105-4123	GO:0031497	denotes	chromatin assembly
T2091	4105-4132	GO:0033186	denotes	chromatin assembly factor 1
T2092	4134-4139	GO:0033186	denotes	CAF-1
T2093	4161-4165	PR:000005402	denotes	p150
T2094	4167-4170	PR:000005403	denotes	p60
T2095	4176-4179	PR:000013776	denotes	p48
T2096	4181-4188	GO:0032991	denotes	complex
T2097	4204-4211	CHEBI:15358	denotes	histone
T2098	4204-4214	PR:000027594	denotes	histone H3
T2099	4204-4211	_FRAGMENT	denotes	histone
T2100	4219-4221	PR:000008603	denotes	H4
T2101	4267-4278	GO:0006260	denotes	replication
T2102	4282-4292	GO:0006281	denotes	DNA repair
T2103	4315-4320	GO:0033186	denotes	CAF-1
T2104	4334-4341	CHEBI:15358	denotes	histone
T2105	4334-4344	PR:000027594	denotes	histone H3
T2106	4363-4372	GO:0000785	denotes	chromatin
T2107	4398-4411	GO:0071897	denotes	DNA synthesis
T2108	4430-4437	PR:000043452	denotes	histone
T2109	4430-4437	CHEBI:15358	denotes	histone
T2110	4449-4453	PR:000008570	denotes	HIRA
T2111	4492-4496	PR:000008425	denotes	H3.3
T2112	4510-4523	GO:0071897	denotes	DNA synthesis
T2113	4582-4591	GO:0000785	denotes	chromatin
T2114	4582-4600	GO:0031497	denotes	chromatin assembly
T2115	4611-4616	GO:0033186	denotes	CAF-1
T2116	4660-4675	GO:0000792	denotes	heterochromatin
T2117	4687-4702	GO:0000792	denotes	heterochromatin
T2118	4687-4712	PR:P05205	denotes	heterochromatin protein 1
T2119	4714-4717	PR:P05205	denotes	HP1
T2120	4723-4727	PR:000010214	denotes	MBD1
T2121	4750-4756	SO:0000417	denotes	domain
T2122	4779-4786	PR:000043452	denotes	histone
T2123	4779-4786	CHEBI:15358	denotes	histone
T2124	4814-4821	PR:000043452	denotes	histone
T2125	4814-4821	CHEBI:15358	denotes	histone
T2126	4873-4882	PR:000005402	denotes	p150CAF-1
T2127	4907-4918	GO:0006260	denotes	replication
T2128	4936-4939	PR:P05205	denotes	HP1
T2129	4940-4949	CHEBI:36357	denotes	molecules
T2130	4953-4964	GO:0006260	denotes	replication
T2131	4953-4970	SO:0000296	denotes	replication sites
T2132	4974-5001	GO:0005721	denotes	pericentric heterochromatin
T2133	5018-5025	GO:0051320	denotes	S phase
T2134	5077-5082	GO:0033186	denotes	CAF-1
T2135	5090-5118	GO:0031507	denotes	formation of heterochromatin
T2136	5103-5118	GO:0000792	denotes	heterochromatin
T2137	5215-5222	PR:000043452	denotes	histone
T2138	5215-5222	CHEBI:15358	denotes	histone
T2139	5357-5364	NCBITaxon:40674	denotes	mammals
T2140	5372-5389	CL:0002248	denotes	pluripotent cells
T2141	5398-5407	UBERON:0000922	denotes	embryonic
T2142	5398-5412	_FRAGMENT	denotes	embryonic stem
T2143	5418-5423	CL:0002322	denotes	cells
T2144	5414-5416	_FRAGMENT	denotes	ES
T2145	5466-5471	GO:0033186	denotes	CAF-1
T2146	5485-5490	NCBITaxon:10088	denotes	mouse
T2147	5506-5513	SO:0000704	denotes	genetic
T2148	5530-5538	CL:0002322	denotes	ES cells
T2149	5558-5574	GO:0016246	denotes	RNA interference
T2150	5576-5580	GO:0016246	denotes	RNAi
T2151	5596-5601	GO:0033186	denotes	CAF-1
T2152	5632-5637	_FRAGMENT	denotes	early
T2153	5644-5651	UBERON:0019248	denotes	embryos
T2154	5638-5643	NCBITaxon:10088	denotes	mouse
T2155	5656-5664	CL:0002322	denotes	ES cells
T2156	5691-5696	GO:0033186	denotes	CAF-1
T2157	5764-5779	GO:0000792	denotes	heterochromatin
T2158	5791-5802	_FRAGMENT	denotes	pluripotent
T2159	5813-5818	CL:0002248	denotes	cells
T2160	5803-5812	UBERON:0000922	denotes	embryonic
T2161	5803-5818	CL:0002321	denotes	embryonic cells
T7310	10980-10984	CHEBI:51231	denotes	DAPI
T7311	11021-11036	GO:0000792	denotes	heterochromatin
T7312	11060-11067	GO:0005634	denotes	nuclear
T7313	11084-11099	GO:0000792	denotes	heterochromatin
T7314	11136-11148	GO:0007566	denotes	implantation
T7315	11174-11182	_FRAGMENT	denotes	two-cell
T7316	11198-11204	UBERON:0007232	denotes	stages
T7317	11187-11197	UBERON:0000358	denotes	blastocyst
T7318	11209-11215	PR:000005402	denotes	Chaf1a
T7319	11223-11230	UBERON:0000922	denotes	embryos
T7320	11263-11269	_FRAGMENT	denotes	eight-
T7321	11277-11287	UBERON:0007236	denotes	cell stage
T7322	11289-11293	CHEBI:51231	denotes	DAPI
T7241	5837-5841	SO:0000704	denotes	gene
T7242	5855-5863	CL:0002322	denotes	ES cells
T7243	5874-5878	SO:0000147	denotes	exon
T7244	5888-5894	PR:000005402	denotes	Chaf1a
T7245	5895-5899	SO:0000704	denotes	gene
T7246	5915-5924	PR:000005402	denotes	p150CAF-1
T7247	5938-5944	PR:000005402	denotes	Chaf1a
T7248	5949-5953	NCBITaxon:10088	denotes	mice
T7249	5959-5963	GO:0007567	denotes	born
T7250	6098-6102	NCBITaxon:10088	denotes	mice
T7251	6137-6143	PR:000005402	denotes	Chaf1a
T7252	6148-6152	NCBITaxon:10088	denotes	mice
T7253	6181-6187	PR:000005402	denotes	Chaf1a
T7254	6192-6199	UBERON:0000922	denotes	embryos
T7255	6233-6245	GO:0007566	denotes	implantation
T7256	6279-6285	PR:000005402	denotes	Chaf1a
T7257	6290-6297	UBERON:0000922	denotes	embryos
T7258	6301-6310	UBERON:0000922	denotes	embryonic
T7259	6386-6393	UBERON:0000922	denotes	embryos
T7260	6408-6414	PR:000005402	denotes	Chaf1a
T7261	6419-6423	NCBITaxon:10088	denotes	mice
T7262	6494-6501	UBERON:0000922	denotes	embryos
T7263	6554-6561	UBERON:0000922	denotes	embryos
T7264	6671-6682	UBERON:0000358	denotes	blastocysts
T7265	6806-6815	PR:000005402	denotes	p150CAF-1
T7266	6825-6832	UBERON:0000922	denotes	embryos
T7267	6844-6853	PR:000005402	denotes	p150CAF-1
T7268	6896-6912	UBERON:0007236	denotes	eight-cell stage
T7269	6997-7003	UBERON:0000922	denotes	embryo
T7270	7014-7029	UBERON:0007233	denotes	four-cell stage
T7271	7050-7059	PR:000005402	denotes	p150CAF-1
T7272	7119-7133	GO:0051301	denotes	cell divisions
T7273	9755-9762	GO:0005634	denotes	nuclear
T7274	9779-9794	GO:0000792	denotes	heterochromatin
T7275	9820-9826	GO:0005634	denotes	nuclei
T7276	9830-9836	PR:000005402	denotes	Chaf1a
T7277	9841-9848	UBERON:0000922	denotes	embryos
T7278	9850-9877	GO:0005721	denotes	Pericentric heterochromatin
T7279	9915-9930	GO:0000792	denotes	heterochromatin
T7280	9960-9970	GO:0051325	denotes	interphase
T7281	9971-9977	GO:0005634	denotes	nuclei
T7282	9985-9997	CHEBI:51217	denotes	fluorochrome
T7283	9998-10002	CHEBI:51231	denotes	DAPI
T7284	10030-10034	PR:000005086	denotes	HP1α
T7285	10060-10071	UBERON:0000358	denotes	blastocysts
T7286	10079-10084	NCBITaxon:10088	denotes	mouse
T7287	10085-10098	CL:0002371	denotes	somatic cells
T7288	10100-10127	GO:0005721	denotes	pericentric heterochromatin
T7289	10175-10184	GO:0000785	denotes	chromatin
T7290	10203-10216	GO:0010369	denotes	chromocenters
T7291	10247-10251	CHEBI:51231	denotes	DAPI
T7292	10314-10320	PR:000005402	denotes	Chaf1a
T7293	10325-10332	UBERON:0000922	denotes	embryos
T7294	10352-10361	GO:0000785	denotes	chromatin
T7295	10421-10427	SO:0001026	denotes	genome
T7296	10545-10560	GO:0000792	denotes	heterochromatin
T7297	10586-10594	_FRAGMENT	denotes	two-cell
T7298	10610-10616	UBERON:0007232	denotes	stages
T7299	10599-10609	UBERON:0000358	denotes	blastocyst
T7300	10621-10635	UBERON:0007232	denotes	two-cell stage
T7301	10646-10653	UBERON:0000922	denotes	embryos
T7302	10655-10659	CHEBI:51231	denotes	DAPI
T7303	10737-10763	GO:0005731	denotes	nucleolar precursor bodies
T7304	10777-10792	GO:0000792	denotes	Heterochromatin
T7305	10834-10838	CHEBI:51231	denotes	DAPI
T7306	10862-10871	_FRAGMENT	denotes	four-cell
T7307	10895-10901	UBERON:0007233	denotes	stages
T7308	10884-10894	UBERON:0000358	denotes	blastocyst
T7309	10931-10935	PR:000005086	denotes	HP1α
T7323	11350-11354	CHEBI:51231	denotes	DAPI
T7324	11387-11394	GO:0005634	denotes	nucleus
T7325	11438-11446	GO:0005730	denotes	nucleoli
T7326	11458-11470	_FRAGMENT	denotes	periphery of
T7327	11475-11481	GO:0034399	denotes	nuclei
T7328	11511-11515	PR:000005086	denotes	HP1α
T7329	11556-11560	CHEBI:51231	denotes	DAPI
T7330	11590-11607	GO:0034399	denotes	nuclear periphery
T7331	11624-11632	GO:0005730	denotes	nucleoli
T7332	11676-11691	GO:0000792	denotes	heterochromatin
T7333	11695-11701	PR:000005402	denotes	Chaf1a
T7334	11706-11713	UBERON:0000922	denotes	embryos
T7335	11736-11751	GO:0000792	denotes	heterochromatin
T7336	11774-11778	_FRAGMENT	denotes	two-
T7337	11787-11797	UBERON:0007232	denotes	cell stage
T7338	11782-11797	UBERON:0007233	denotes	four-cell stage
T7339	11808-11815	UBERON:0000922	denotes	embryos
T7340	11879-11888	PR:000005402	denotes	p150CAF-1
T7341	11900-11912	GO:0007566	denotes	implantation
T7342	12002-12024	_FRAGMENT	denotes	heterochromatin within
T7343	12040-12046	GO:0005720	denotes	nuclei
T7344	12025-12034	UBERON:0000922	denotes	embryonic
T7345	12025-12039	CL:0002321	denotes	embryonic cell
T7346	12035-12046	GO:0005634	denotes	cell nuclei
T7347	12100-12105	GO:0033186	denotes	CAF-1
T7348	12132-12140	CL:0002322	denotes	ES cells
T7349	12169-12179	UBERON:0000358	denotes	blastocyst
T7350	12180-12195	UBERON:0000087	denotes	inner cell mass
T7351	12246-12252	PR:000005402	denotes	Chaf1a
T7352	12257-12264	UBERON:0000922	denotes	embryos
T7353	12317-12324	UBERON:0000922	denotes	embryos
T7354	12346-12350	GO:0016246	denotes	RNAi
T7355	12373-12382	PR:000005402	denotes	p150CAF-1
T7356	12396-12404	CL:0002322	denotes	ES cells
T7357	12466-12475	PR:000005402	denotes	p150CAF-1
T7358	12476-12480	GO:0016246	denotes	RNAi
T7359	12490-12498	CL:0002322	denotes	ES cells
T7360	12550-12556	PR:000005402	denotes	Chaf1a
T7361	12564-12568	NCBITaxon:10088	denotes	mice
T7362	12570-12574	CHEBI:51231	denotes	DAPI
T7363	12621-12625	PR:000005086	denotes	HP1α
T7364	12630-12634	CHEBI:51231	denotes	DAPI
T7365	12655-12663	GO:0005730	denotes	nucleoli
T7366	12675-12687	_FRAGMENT	denotes	periphery of
T7367	12692-12699	GO:0034399	denotes	nucleus
T7368	12740-12749	PR:000005402	denotes	p150CAF-1
T7369	12767-12774	GO:0005634	denotes	nuclear
T7370	12791-12806	GO:0000792	denotes	heterochromatin
T7371	12810-12818	CL:0002322	denotes	ES cells
T7372	12849-12861	GO:0007566	denotes	implantation
T7373	12862-12869	UBERON:0000922	denotes	embryos
T7374	12930-12945	GO:0000792	denotes	heterochromatin
T7375	12970-12975	NCBITaxon:10088	denotes	mouse
T7376	12976-12985	UBERON:0000922	denotes	embryonic
T7377	12986-12997	CL:0000057	denotes	fibroblasts
T7378	13015-13024	PR:000005402	denotes	p150CAF-1
T7379	13038-13042	GO:0016246	denotes	RNAi
T7380	13044-13053	PR:000005402	denotes	p150CAF-1
T7381	13107-13125	GO:0008283	denotes	cell proliferation
T7382	13182-13197	GO:0000792	denotes	heterochromatin
T7383	13238-13247	PR:000005402	denotes	p150CAF-1
T7384	13286-13301	GO:0000792	denotes	heterochromatin
T7385	13371-13376	GO:0033186	denotes	CAF-1
T7386	13384-13396	UBERON:0019248	denotes	early embryo
T7387	13401-13409	CL:0002322	denotes	ES cells
T7388	14716-14725	PR:000005402	denotes	p150CAF-1
T7389	14729-14737	CL:0002322	denotes	ES cells
T7390	14763-14778	GO:0000792	denotes	heterochromatin
T7391	14779-14786	GO:0005634	denotes	nuclear
T7392	14855-14862	GO:0005634	denotes	nuclear
T7393	14913-14921	GO:0042571	denotes	antibody
T7394	14934-14947	CL:0000836	denotes	promyelocytic
T7395	14934-14956	PR:000026474	denotes	promyelocytic leukemia
T7396	14958-14961	PR:000026474	denotes	PML
T7397	14996-14999	PR:000026474	denotes	PML
T7398	14996-15014	GO:0016605	denotes	PML nuclear bodies
T7399	15048-15055	GO:0005634	denotes	nuclear
T7400	15154-15157	PR:000026474	denotes	PML
T7401	15154-15172	GO:0016605	denotes	PML nuclear bodies
T7402	15193-15202	PR:000005402	denotes	p150CAF-1
T7403	15212-15220	CL:0002322	denotes	ES cells
T7404	15255-15262	GO:0005634	denotes	nuclear
T7405	15310-15315	GO:0033186	denotes	CAF-1
T7406	15383-15388	GO:0033186	denotes	CAF-1
T7407	15435-15444	GO:0000785	denotes	chromatin
T7408	15476-15483	CHEBI:15358	denotes	histone
T7409	15476-15486	PR:000027594	denotes	histone H3
T7410	15491-15495	PR:000005086	denotes	HP1α
T7411	15515-15520	GO:0033186	denotes	CAF-1
T7412	15549-15562	UBERON:0019248	denotes	early embryos
T7413	15570-15578	CL:0002322	denotes	ES cells
T7414	15614-15629	GO:0000792	denotes	heterochromatin
T7415	15633-15640	GO:0005634	denotes	nuclear
T7416	15684-15693	NCBITaxon:40674	denotes	mammalian
T7417	15717-15722	GO:0033186	denotes	CAF-1
T7418	15748-15755	GO:0051320	denotes	S phase
T7419	15808-15820	GO:0009294	denotes	transfection
T7420	15828-15832	GO:0016246	denotes	RNAi
T7421	15833-15839	SO:0000440	denotes	vector
T7422	15841-15850	PR:000005402	denotes	p150CAF-1
T7423	15903-15908	GO:0033186	denotes	CAF-1
T7424	15944-15955	GO:0006260	denotes	replication
T7425	16010-16027	CHEBI:472552	denotes	bromodeoxyuridine
T7426	16029-16033	CHEBI:472552	denotes	BrdU
T7427	16052-16056	PR:000012421	denotes	PCNA
T7428	16058-16071	GO:0008283	denotes	proliferating
T7429	16058-16092	PR:000012421	denotes	proliferating cell nuclear antigen
T7430	16085-16092	CHEBI:59132	denotes	antigen
T7431	16179-16194	GO:0006260	denotes	DNA replication
T7432	16235-16250	GO:0000792	denotes	heterochromatin
T7433	16286-16294	CL:0002322	denotes	ES cells
T7434	16304-16315	GO:0008283	denotes	proliferate
T7435	16320-16323	GO:0016265	denotes	die
T7436	16340-16349	PR:000005402	denotes	p150CAF-1
T7437	16379-16395	GO:0009987	denotes	cellular process
T7438	16403-16410	UBERON:0000922	denotes	embryos
T7439	16458-16467	PR:000005402	denotes	p150CAF-1
T7440	16480-16488	CL:0002322	denotes	ES cells
T7441	16493-16506	UBERON:0019248	denotes	early embryos
T7442	16540-16558	_FRAGMENT	denotes	heterochromatin in
T7443	16563-16570	GO:0005720	denotes	nucleus
T7444	16598-16608	GO:0007049	denotes	cell cycle
T7445	17892-17907	GO:0000792	denotes	heterochromatin
T7446	17970-17983	GO:0097617	denotes	hybridization
T7447	18095-18100	NCBITaxon:10088	denotes	mouse
T7448	18317-18321	CHEBI:51231	denotes	DAPI
T7449	18340-18350	GO:0051325	denotes	interphase
T7450	18351-18358	GO:0005634	denotes	nucleus
T7451	18474-18483	PR:000005402	denotes	p150CAF-1
T7452	18484-18488	GO:0016246	denotes	RNAi
T7453	18533-18560	GO:0005721	denotes	pericentric heterochromatin
T7454	18611-18615	CHEBI:51231	denotes	DAPI
T7455	18874-18891	GO:0034399	denotes	nuclear periphery
T7456	18914-18918	CHEBI:51231	denotes	DAPI
T7457	18954-18963	PR:000005402	denotes	p150CAF-1
T7458	19052-19059	GO:0005634	denotes	nucleus
T7459	19128-19132	CHEBI:51231	denotes	DAPI
T7460	19153-19166	GO:0097617	denotes	hybridization
T7461	19178-19187	PR:000005402	denotes	p150CAF-1
T7462	19252-19279	GO:0005721	denotes	pericentric heterochromatin
T7463	19416-19425	PR:000005402	denotes	p150CAF-1
T7464	19495-19504	PR:000005402	denotes	p150CAF-1
T7465	19563-19590	GO:0005721	denotes	pericentric heterochromatin
T7466	21346-21351	GO:0033186	denotes	CAF-1
T7467	21373-21380	CHEBI:15358	denotes	histone
T7468	21373-21380	_FRAGMENT	denotes	histone
T7469	21390-21392	PR:000008603	denotes	H4
T7470	21409-21422	GO:0071897	denotes	DNA synthesis
T7471	21480-21489	GO:0000785	denotes	chromatin
T7472	21529-21540	GO:0000786	denotes	nucleosomal
T7473	21678-21687	PR:000005402	denotes	p150CAF-1
T7474	21697-21705	CL:0002322	denotes	ES cells
T7475	21727-21733	SO:0001026	denotes	genome
T7476	21734-21743	GO:0000785	denotes	chromatin
T7477	21848-21855	CHEBI:15358	denotes	histone
T7478	21848-21858	PR:000027594	denotes	histone H3
T7479	21864-21883	GO:0000790	denotes	chromatin in nuclei
T7480	21904-21915	GO:0009294	denotes	transfected
T7481	21933-21942	PR:000005402	denotes	p150CAF-1
T7482	21943-21947	GO:0016246	denotes	RNAi
T7483	21948-21963	SO:0000755	denotes	plasmid vectors
T7484	21965-21971	GO:0005634	denotes	Nuclei
T7485	22013-22017	CHEBI:24866	denotes	salt
T7486	22053-22057	CHEBI:26710	denotes	NaCl
T7487	22065-22069	CHEBI:26710	denotes	NaCl
T7488	22104-22111	CHEBI:15358	denotes	histone
T7489	22104-22114	PR:000027594	denotes	histone H3
T7490	22141-22150	GO:0000785	denotes	chromatin
T7491	22188-22197	PR:000005402	denotes	p150CAF-1
T7492	22207-22215	CL:0002322	denotes	ES cells
T7493	22292-22319	GO:0005721	denotes	pericentric heterochromatin
T7494	22376-22387	GO:0000786	denotes	nucleosomal
T7495	22970-22977	GO:0005634	denotes	nuclear
T7496	22994-22998	PR:000005086	denotes	HP1α
T7497	23022-23028	PR:000005402	denotes	Chaf1a
T7498	23033-23040	UBERON:0000922	denotes	embryos
T7499	23045-23054	PR:000005402	denotes	p150CAF-1
T7500	23064-23072	CL:0002322	denotes	ES cells
T7501	23166-23193	GO:0005721	denotes	pericentric heterochromatin
T7502	23211-23230	GO:0010424	denotes	DNA CpG methylation
T7503	23319-23328	PR:000005402	denotes	p150CAF-1
T7504	23368-23375	CL:0002322	denotes	ES cell
T7505	23401-23405	GO:0016246	denotes	RNAi
T7506	23446-23461	GO:0006306	denotes	DNA methylation
T7507	23618-23633	GO:0000792	denotes	heterochromatin
T7508	23645-23654	PR:000005402	denotes	p150CAF-1
T7509	23664-23672	CL:0002322	denotes	ES cells
T7510	23688-23692	GO:0016246	denotes	RNAi
T7511	23693-23707	SO:0000755	denotes	plasmid vector
T7512	23716-23723	CL:0002322	denotes	ES cell
T7513	23757-23766	PR:000005402	denotes	p150CAF-1
T7514	23790-23805	GO:0000792	denotes	heterochromatin
T7515	23850-23865	GO:0006306	denotes	DNA methylation
T7516	23935-23942	PR:000043452	denotes	histone
T7517	23935-23942	CHEBI:15358	denotes	histone
T7518	23935-23956	GO:0016570	denotes	histone modifications
T7519	23965-23974	GO:0000785	denotes	chromatin
T7520	24027-24036	GO:0000785	denotes	chromatin
T7521	24149-24159	GO:0042571	denotes	antibodies
T7522	24170-24179	PR:000005402	denotes	p150CAF-1
T7523	24332-24341	PR:000005402	denotes	p150CAF-1
T7524	24378-24405	GO:0005721	denotes	pericentric heterochromatin
T7525	24649-24658	PR:000005402	denotes	p150CAF-1
T7526	24703-24730	GO:0005721	denotes	pericentric heterochromatin
T7527	24771-24778	SO:0001707	denotes	H3K9me3
T7528	24823-24832	PR:000005402	denotes	p150CAF-1
T7529	26696-26703	SO:0001707	denotes	H3K9me3
T7530	26740-26744	CHEBI:51231	denotes	DAPI
T7531	26807-26814	SO:0001707	denotes	H3K9me3
T7532	26841-26850	PR:000005402	denotes	p150CAF-1
T7533	26907-26911	PR:000005086	denotes	HP1α
T7534	27198-27202	CHEBI:51231	denotes	DAPI
T7535	27266-27271	GO:0033186	denotes	CAF-1
T7536	27275-27283	CL:0002322	denotes	ES cells
T7537	27322-27337	GO:0000792	denotes	heterochromatin
T12672	27439-27448	PR:000005402	denotes	p150CAF-1
T12673	27479-27484	NCBITaxon:10088	denotes	mouse
T12674	27491-27504	GO:0009790	denotes	embryogenesis
T12675	27512-27520	CL:0002322	denotes	ES cells
T12676	27551-27556	GO:0033186	denotes	CAF-1
T12677	27593-27608	GO:0006260	denotes	DNA replication
T12678	27612-27623	NCBITaxon:7742	denotes	vertebrates
T12679	27628-27642	NCBITaxon:8355	denotes	Xenopus laevis
T12680	27644-27653	PR:000005402	denotes	p150CAF-1
T12681	27689-27703	GO:0051301	denotes	cell divisions
T12682	27757-27762	GO:0033186	denotes	CAF-1
T12683	27779-27788	NCBITaxon:40674	denotes	mammalian
T12684	27823-27838	GO:0006260	denotes	DNA replication
T12685	27885-27890	GO:0033186	denotes	CAF-1
T12686	27898-27905	GO:0005634	denotes	nuclear
T12687	27922-27937	GO:0000792	denotes	heterochromatin
T12688	27978-27986	CL:0002322	denotes	ES cells
T12689	28004-28013	PR:000005402	denotes	p150CAF-1
T12690	28023-28031	CL:0002322	denotes	ES cells
T12691	28039-28054	GO:0006260	denotes	DNA replication
T12692	28110-28121	GO:0000786	denotes	nucleosomal
T12693	28143-28158	GO:0000792	denotes	heterochromatin
T12694	28205-28214	PR:000005402	denotes	p150CAF-1
T12695	28230-28233	PR:000026474	denotes	PML
T12696	28230-28240	GO:0016605	denotes	PML bodies
T12697	28244-28251	CL:0002322	denotes	ES cell
T12698	28247-28258	GO:0005634	denotes	cell nuclei
T12699	28273-28280	GO:0005634	denotes	nuclear
T12700	28332-28341	PR:000005402	denotes	p150CAF-1
T12701	28377-28384	CL:0002322	denotes	ES cell
T12702	28380-28393	GO:0051301	denotes	cell division
T12703	28398-28408	GO:0008219	denotes	cell death
T12704	28446-28461	GO:0000792	denotes	heterochromatin
T12705	28561-28568	UBERON:0000922	denotes	embryos
T12706	28573-28578	GO:0016265	denotes	death
T12707	28582-28590	CL:0002322	denotes	ES cells
T12708	28604-28609	GO:0033186	denotes	CAF-1
T12709	28617-28624	GO:0005634	denotes	nuclear
T12710	28641-28656	GO:0000792	denotes	heterochromatin
T12711	28768-28773	GO:0033186	denotes	CAF-1
T12712	28822-28827	NCBITaxon:9606	denotes	human
T12713	28874-28885	_FRAGMENT	denotes	pluripotent
T12714	28896-28901	CL:0002248	denotes	cells
T12715	28886-28895	UBERON:0000922	denotes	embryonic
T12716	28886-28901	CL:0002321	denotes	embryonic cells
T12717	28918-28931	UBERON:0019248	denotes	early embryos
T12718	28936-28944	CL:0002322	denotes	ES cells
T12719	28952-28958	SO:0001026	denotes	genome
T12720	28980-28993	CL:0002371	denotes	somatic cells
T12721	29071-29076	GO:0033186	denotes	CAF-1
T12722	29080-29095	GO:0000792	denotes	heterochromatin
T12723	29128-29136	CL:0002322	denotes	ES cells
T12724	29158-29167	GO:0000785	denotes	chromatin
T12725	29225-29234	GO:0000785	denotes	chromatin
T12726	29297-29312	GO:0000792	denotes	heterochromatin
T12727	29313-29320	GO:0005634	denotes	nuclear
T12728	29344-29349	GO:0033186	denotes	CAF-1
T12729	29449-29455	SO:0001026	denotes	genome
T12730	29498-29506	CL:0002322	denotes	ES cells
T12731	29539-29544	GO:0033186	denotes	CAF-1
T12732	29583-29588	GO:0033186	denotes	CAF-1
T12733	29592-29603	_FRAGMENT	denotes	pluripotent
T12734	29614-29619	CL:0002248	denotes	cells
T12735	29604-29613	UBERON:0000922	denotes	embryonic
T12736	29604-29619	CL:0002321	denotes	embryonic cells
T12737	29678-29698	NCBITaxon:3702	denotes	Arabidopsis thaliana
T12738	29709-29714	GO:0033186	denotes	CAF-1
T12739	29760-29765	GO:0033186	denotes	CAF-1
T12740	29806-29818	GO:0010369	denotes	chromocenter
T12741	29879-29884	GO:0033186	denotes	CAF-1
T12742	29905-29920	GO:0000792	denotes	heterochromatin
T12743	29937-29942	_FRAGMENT	denotes	early
T12744	29949-29956	UBERON:0019248	denotes	embryos
T12745	29943-29948	NCBITaxon:10088	denotes	mouse
T12746	29961-29966	NCBITaxon:10088	denotes	mouse
T12747	29967-29975	CL:0002322	denotes	ES cells
T12748	29990-29995	GO:0033186	denotes	CAF-1
T12749	30142-30147	NCBITaxon:10088	denotes	mouse
T12750	30148-30154	SO:0001026	denotes	genome
T12751	30187-30194	GO:0005634	denotes	nuclear
T12752	30234-30261	GO:0005721	denotes	pericentric heterochromatin
T12753	30277-30285	CL:0002322	denotes	ES cells
T12754	30322-30337	GO:0000792	denotes	heterochromatin
T12755	30406-30411	GO:0033186	denotes	CAF-1
T12756	30442-30447	GO:0033186	denotes	CAF-1
T12757	30455-30462	NCBITaxon:species	denotes	species
T12758	30537-30544	CHEBI:15358	denotes	histone
T12759	30537-30547	PR:000027594	denotes	histone H3
T12760	30577-30587	NCBITaxon:2759	denotes	eukaryotes
T12761	30589-30594	GO:0033186	denotes	CAF-1
T12762	30611-30618	NCBITaxon:40674	denotes	mammals
T12763	30638-30662	NCBITaxon:4932	denotes	Saccharomyces cerevisiae
T12764	30688-30695	GO:0007114	denotes	budding
T12765	30688-30701	NCBITaxon:4892	denotes	budding yeast
T12766	30702-30708	SO:0001026	denotes	genome
T12767	30726-30741	GO:0000792	denotes	heterochromatin
T12768	30780-30790	NCBITaxon:2759	denotes	eukaryotes
T12769	30825-30840	GO:0000792	denotes	heterochromatin
T12770	30849-30852	_FRAGMENT	denotes	di-
T12771	30860-30879	GO:0036123	denotes	methylation at H3K9
T12772	30860-30874	_FRAGMENT	denotes	methylation at
T12773	30892-30897	GO:0034772	denotes	H4K20
T12774	30857-30879	GO:0036124	denotes	trimethylation at H3K9
T12775	30857-30874	_FRAGMENT	denotes	trimethylation at
T12776	30881-30886	GO:0070734	denotes	H3K27
T12777	30892-30897	GO:0034773	denotes	H4K20
T12778	30922-30938	GO:0035328	denotes	silent chromatin
T12779	30942-30955	NCBITaxon:4932	denotes	S. cerevisiae
T12780	30960-30967	NCBITaxon:40674	denotes	mammals
T12781	30969-30987	GO:0036123	denotes	H3K9 dimethylation
T12782	31040-31044	PR:000008425	denotes	H3.3
T12783	31105-31114	GO:0000785	denotes	chromatin
T12784	31156-31158	PR:000027594	denotes	H3
T12785	31168-31181	NCBITaxon:4932	denotes	S. cerevisiae
T12786	31197-31201	PR:000008425	denotes	H3.3
T12787	31212-31221	GO:0010467	denotes	expressed
T12788	31244-31253	GO:0000785	denotes	chromatin
T12789	31259-31270	GO:0006260	denotes	replication
T12790	31301-31311	NCBITaxon:2759	denotes	eukaryotes
T12791	31365-31380	GO:0000792	denotes	heterochromatin
T12792	31408-31413	GO:0033186	denotes	CAF-1
T12793	31431-31442	GO:0006260	denotes	replication
T12794	31461-31468	GO:0032991	denotes	complex
T12795	31508-31515	GO:0005634	denotes	nuclear
T12796	31532-31559	GO:0005721	denotes	pericentric heterochromatin
T12797	31563-31570	UBERON:0000922	denotes	embryos
T12798	31575-31583	CL:0002322	denotes	ES cells
T12799	31657-31666	GO:0000785	denotes	chromatin
T12800	31694-31701	GO:0005634	denotes	nuclear
T12801	31731-31746	GO:0000792	denotes	heterochromatin
T12802	31753-31762	UBERON:0000922	denotes	embryonic
T12803	31916-31931	GO:0000792	denotes	heterochromatin
T12804	31943-31952	PR:000005402	denotes	p150CAF-1
T12805	31958-31965	UBERON:0000922	denotes	embryos
T12806	31970-31978	CL:0002322	denotes	ES cells
T12807	31980-31985	GO:0033186	denotes	CAF-1
T12808	32026-32041	GO:0000792	denotes	heterochromatin
T12809	32152-32167	GO:0006260	denotes	DNA replication
T12810	32186-32195	PR:000005402	denotes	p150CAF-1
T12811	32197-32212	GO:0000792	denotes	heterochromatin
T12812	32243-32251	CHEBI:36357	denotes	molecule
T12813	32374-32379	GO:0033186	denotes	CAF-1
T12814	32445-32460	GO:0000792	denotes	heterochromatin
T12815	32468-32480	GO:0007566	denotes	implantation
T12816	32481-32488	UBERON:0000922	denotes	embryos
T12817	32521-32528	GO:0005634	denotes	nuclear
T12818	32554-32562	_FRAGMENT	denotes	two-cell
T12819	32578-32584	UBERON:0007232	denotes	stages
T12820	32567-32577	UBERON:0000358	denotes	blastocyst
T12821	32609-32614	NCBITaxon:10088	denotes	mouse
T12822	32633-32640	GO:0005634	denotes	nuclear
T12823	32657-32675	_FRAGMENT	denotes	heterochromatin in
T12824	32684-32690	GO:0005720	denotes	nuclei
T12825	32676-32683	CL:0002322	denotes	ES cell
T12826	32679-32690	GO:0005634	denotes	cell nuclei
T12827	32721-32735	UBERON:0000106	denotes	one-cell stage
T12828	32756-32763	GO:0005634	denotes	nuclear
T12829	32800-32815	GO:0000792	denotes	heterochromatin
T12830	32843-32850	GO:0005634	denotes	nuclear
T12831	32875-32890	UBERON:0019248	denotes	early embryonic
T12832	32908-32915	UBERON:0000922	denotes	embryos
T12833	32948-32963	GO:0000792	denotes	heterochromatin
T12834	33008-33017	PR:000005402	denotes	p150CAF-1
T12835	33027-33035	CL:0002322	denotes	ES cells
T12836	33083-33089	PR:000005402	denotes	Chaf1a
T12837	33093-33100	UBERON:0000922	denotes	embryos
T12838	33123-33128	GO:0033186	denotes	CAF-1
T12839	33162-33177	GO:0000792	denotes	heterochromatin
T12840	33208-33222	GO:0051301	denotes	cell divisions
T12841	33226-33235	UBERON:0000922	denotes	embryonic
T12842	33266-33273	GO:0005634	denotes	nuclear
T12843	33294-33320	GO:0010468	denotes	control of gene expression
T12844	33305-33309	SO:0000704	denotes	gene
T12845	33357-33362	GO:0033186	denotes	CAF-1
T12846	33406-33410	SO:0000704	denotes	gene
T12847	33406-33421	GO:0010467	denotes	gene expression
T12848	33435-33448	GO:0009790	denotes	embryogenesis
T12849	33509-33518	GO:0000785	denotes	chromatin
T12850	33560-33571	_FRAGMENT	denotes	pluripotent
T12851	33582-33587	CL:0002248	denotes	cells
T12852	33572-33581	UBERON:0000922	denotes	embryonic
T12853	33572-33587	CL:0002321	denotes	embryonic cells
T12854	33631-33646	GO:0006306	denotes	DNA methylation
T12855	33651-33658	PR:000043452	denotes	histone
T12856	33651-33658	CHEBI:15358	denotes	histone
T12857	33651-33668	GO:0016570	denotes	histone modifying
T12858	33722-33731	GO:0000785	denotes	chromatin
T12859	33722-33740	GO:0031497	denotes	chromatin assembly
T12860	33791-33802	GO:0000786	denotes	nucleosomes
T12861	33804-33809	GO:0033186	denotes	CAF-1
T12862	33857-33872	GO:0000792	denotes	heterochromatin
T12863	33884-33897	UBERON:0019248	denotes	early embryos
T12864	33902-33910	CL:0002322	denotes	ES cells
T14739	33936-33949	_FRAGMENT	denotes	Generation of
T14740	33973-33980	GO:0009790	denotes	embryos
T14741	33950-33956	PR:000005402	denotes	Chaf1a
T14742	33964-33968	NCBITaxon:10088	denotes	mice
T14743	33973-33980	UBERON:0000922	denotes	embryos
T14744	34010-34017	SO:0001026	denotes	genomic
T14745	34046-34054	SO:0000357	denotes	flanking
T14746	34055-34061	PR:000005402	denotes	Chaf1a
T14747	34062-34066	SO:0000147	denotes	exon
T14748	34138-34146	CHEBI:7507	denotes	neomycin
T14749	34166-34171	CHEBI:27026	denotes	toxin
T14750	34173-34182	SO:0005853	denotes	cassettes
T14751	34229-34240	GO:0009294	denotes	transfected
T14752	34244-34252	CL:0002322	denotes	ES cells
T14753	34299-34307	CL:0002322	denotes	ES cells
T14754	34328-34334	PR:000005402	denotes	Chaf1a
T14755	34355-34361	PR:000005402	denotes	Chaf1a
T14756	34383-34389	SO:0001023	denotes	allele
T14757	34431-34437	PR:000005402	denotes	Chaf1a
T14758	34442-34446	NCBITaxon:10088	denotes	mice
T14759	34471-34479	CL:0002322	denotes	ES cells
T14760	34494-34505	UBERON:0000358	denotes	blastocysts
T14761	34510-34517	UBERON:0000922	denotes	embryos
T14762	34550-34556	PR:000005402	denotes	Chaf1a
T14763	34561-34565	NCBITaxon:10088	denotes	mice
T14764	34610-34617	UBERON:0000922	denotes	Embryos
T14765	34651-34654	CHEBI:60004	denotes	mix
T14766	34682-34689	SO:0000112	denotes	primers
T14767	34752-34759	SO:0001023	denotes	alleles
T14768	34903-34910	SO:0000112	denotes	primers
T14769	34977-34984	CHEBI:2511	denotes	agarose
T14770	35040-35042	SO:0000028	denotes	bp
T14771	35065-35067	SO:0000028	denotes	bp
T14772	35069-35076	SO:0001023	denotes	alleles
T15607	35079-35088	PR:000005402	denotes	P150CAF-1
T15608	35102-35106	GO:0016246	denotes	RNAi
T15609	35112-35116	GO:0016246	denotes	RNAi
T15610	35117-35131	SO:0000755	denotes	plasmid vector
T15611	35172-35177	NCBITaxon:10088	denotes	mouse
T15612	35181-35189	SO:0000167	denotes	promoter
T15613	35196-35205	CHEBI:17939	denotes	puromycin
T15614	35216-35220	SO:0000704	denotes	gene
T15615	35306-35312	SO:0000440	denotes	vector
T15616	35332-35336	SO:0000704	denotes	gene
T15617	35368-35373	SO:0000704	denotes	genes
T15618	35377-35385	CL:0002322	denotes	ES cells
T15619	35534-35543	PR:000005402	denotes	p150CAF-1
T15620	35544-35549	SO:0000646	denotes	siRNA
T15621	35551-35572	SO:0000646	denotes	short interfering RNA
T15622	35607-35614	SO:0000155	denotes	plasmid
T15623	35628-35634	SO:0000440	denotes	vector
T15624	35635-35644	GO:0010467	denotes	expressed
T15625	35647-35652	SO:0000646	denotes	siRNA
T15626	35670-35685	GO:0006401	denotes	RNA degradation
T15627	35687-35695	CL:0002322	denotes	ES cells
T15628	35701-35712	GO:0009294	denotes	transfected
T15629	35745-35759	SO:0000755	denotes	plasmid vector
T15630	35773-35780	CHEBI:5291	denotes	gelatin
T15631	35847-35856	CHEBI:17939	denotes	Puromycin
T15632	35964-35973	PR:000005402	denotes	p150CAF-1
T15633	35974-35978	GO:0016246	denotes	RNAi
T15634	35979-35985	SO:0000440	denotes	vector
T15635	36035-36040	GO:0033186	denotes	CAF-1
T15636	36131-36140	PR:000005402	denotes	p150CAF-1
T15637	36141-36151	GO:0010467	denotes	expression
T15638	36218-36222	SO:0000704	denotes	gene
T15639	36262-36273	GO:0009294	denotes	transfected
T15640	36294-36298	GO:0016246	denotes	RNAi
T15641	36299-36313	SO:0000755	denotes	plasmid vector
T15642	36519-36528	CHEBI:17939	denotes	puromycin
T16153	36548-36556	CL:0002322	denotes	ES cells
T16154	36598-36602	SO:0000704	denotes	gene
T16155	36619-36627	CL:0002322	denotes	ES cells
T16156	36645-36649	GO:0016246	denotes	RNAi
T16157	36758-36766	CL:0002322	denotes	ES cells
T16158	36841-36848	UBERON:0000922	denotes	embryos
T16554	37020-37027	UBERON:0000922	denotes	embryos
T16555	37067-37071	CHEBI:37527	denotes	acid
T16556	37086-37100	UBERON:0000086	denotes	zona pellucida
T16557	37186-37194	CL:0002322	denotes	ES cells
T16558	37196-37206	GO:0042571	denotes	Antibodies
T16559	37212-37216	PR:000005086	denotes	HP1α
T16560	37301-37305	CHEBI:472552	denotes	BrdU
T16561	37348-37352	PR:000012421	denotes	PCNA
T16562	37380-37383	PR:000026474	denotes	PML
T16563	37480-37490	GO:0042571	denotes	Antibodies
T16564	37496-37501	NCBITaxon:10088	denotes	mouse
T16565	37502-37510	PR:000005402	denotes	p150CAF1
T16566	37525-37532	SO:0001707	denotes	H3K9me3
T16567	37615-37625	GO:0042571	denotes	antibodies
T16904	37738-37743	GO:0019835	denotes	lysed
T16905	37783-37786	CHEBI:8984	denotes	SDS
T16906	37814-37824	GO:0042571	denotes	antibodies
T16907	37833-37842	PR:000005402	denotes	p150CAF-1
T16908	37867-37871	PR:000005086	denotes	HP1α
T16909	37897-37904	CHEBI:15358	denotes	Histone
T16910	37897-37907	PR:000027594	denotes	Histone H3
T16911	37924-37931	SO:0001707	denotes	H3K9me3
T16912	37953-37960	PR:000003676	denotes	β-actin
T17159	38032-38038	CHEBI:15956	denotes	Biotin
T17160	38050-38061	CHEBI:42098	denotes	Digoxigenin
T17161	38136-38147	GO:0006412	denotes	translation
T17840	38389-38395	GO:0005634	denotes	Nuclei
T17841	38457-38466	GO:0000785	denotes	chromatin
T17842	38468-38476	CL:0002322	denotes	ES cells
T17843	38529-38533	CHEBI:9754	denotes	Tris
T17844	38534-38537	CHEBI:17883	denotes	HCl
T17845	38554-38561	CHEBI:17992	denotes	sucrose
T17846	38569-38572	CHEBI:32588	denotes	KCl
T17847	38580-38584	CHEBI:26710	denotes	NaCl
T17848	38591-38596	CHEBI:6636	denotes	MgCl2
T17849	38674-38680	GO:0005634	denotes	Nuclei
T17850	38749-38756	CHEBI:17992	denotes	sucrose
T17851	38787-38794	CHEBI:17992	denotes	sucrose
T17852	38838-38842	CHEBI:9754	denotes	Tris
T17853	38843-38846	CHEBI:17883	denotes	HCl
T17854	38863-38867	CHEBI:26710	denotes	NaCl
T17855	38876-38883	CHEBI:17992	denotes	sucrose
T17856	38890-38895	CHEBI:6636	denotes	MgCl2
T17857	38902-38907	CHEBI:3312	denotes	CaCl2
T17858	38922-38928	GO:0005634	denotes	nuclei
T17859	39127-39130	CHEBI:8984	denotes	SDS
T17860	39213-39219	CHEBI:15882	denotes	phenol
T17861	39220-39230	CHEBI:35255	denotes	chloroform
T17862	39246-39257	CHEBI:17824	denotes	isopropanol
T17863	39300-39307	CHEBI:15358	denotes	histone
T17864	39300-39310	PR:000027594	denotes	histone H3
T17865	39316-39325	GO:0000785	denotes	chromatin
T17866	39341-39345	PR:000005402	denotes	p150
T17867	39371-39377	GO:0005634	denotes	nuclei
T17868	39430-39435	CHEBI:46756	denotes	Hepes
T17869	39449-39457	CHEBI:17754	denotes	Glycerol
T17870	39464-39469	CHEBI:6636	denotes	MgCl2
T17871	39491-39494	CHEBI:18320	denotes	DTT
T17872	39503-39507	CHEBI:8102	denotes	PMSF
T17873	39570-39574	CHEBI:26710	denotes	NaCl
T17874	39671-39678	CHEBI:15358	denotes	histone
T17875	39671-39681	PR:000027594	denotes	histone H3
T17876	39682-39690	GO:0042571	denotes	antibody
T18595	39726-39735	GO:0000785	denotes	chromatin
T18596	39760-39771	GO:0000786	denotes	nucleosomes
T18597	39809-39818	GO:0000785	denotes	chromatin
T18598	39869-39879	GO:0042571	denotes	antibodies
T18599	39992-39999	CHEBI:2511	denotes	agarose
T18600	40036-40049	GO:0097617	denotes	Hybridization
T18601	40151-40161	GO:0042571	denotes	Antibodies
T18602	40211-40221	GO:0042571	denotes	Antibodies
T18603	40226-40233	SO:0001707	denotes	H3K9me3
T18604	40359-40361	SO:0000028	denotes	bp
T18605	40368-40373	PR:P23940	denotes	BamHI
T18606	40435-40437	SO:0000028	denotes	bp
T18607	40444-40451	PR:P43870	denotes	HindIII
T18608	40513-40515	SO:0000028	denotes	bp
R321	T2143	T2142	_lexicallyChainedTo	cells,embryonic stem
R322	T2143	T2144	_lexicallyChainedTo	cells,ES
R323	T2153	T2152	_lexicallyChainedTo	embryos,early
R324	T2159	T2158	_lexicallyChainedTo	cells,pluripotent
R782	T7443	T7442	_lexicallyChainedTo	nucleus,heterochromatin in
R783	T7298	T7297	_lexicallyChainedTo	stages,two-cell
R784	T7307	T7306	_lexicallyChainedTo	stages,four-cell
R785	T7316	T7315	_lexicallyChainedTo	stages,two-cell
R786	T7321	T7320	_lexicallyChainedTo	cell stage,eight-
R787	T7327	T7326	_lexicallyChainedTo	nuclei,periphery of
R788	T7337	T7336	_lexicallyChainedTo	cell stage,two-
R789	T7343	T7342	_lexicallyChainedTo	nuclei,heterochromatin within
R790	T7469	T7468	_lexicallyChainedTo	H4,histone
R791	T7367	T7366	_lexicallyChainedTo	nucleus,periphery of
R3058	T12714	T12713	_lexicallyChainedTo	cells,pluripotent
R3059	T12734	T12733	_lexicallyChainedTo	cells,pluripotent
R3060	T12744	T12743	_lexicallyChainedTo	embryos,early
R3061	T12771	T12770	_lexicallyChainedTo	methylation at H3K9,di-
R3062	T12772	T12770	_lexicallyChainedTo	methylation at,di-
R3063	T12773	T12772	_lexicallyChainedTo	H4K20,methylation at
R3064	T12776	T12775	_lexicallyChainedTo	H3K27,trimethylation at
R3065	T12777	T12775	_lexicallyChainedTo	H4K20,trimethylation at
R3066	T12819	T12818	_lexicallyChainedTo	stages,two-cell
R3067	T12824	T12823	_lexicallyChainedTo	nuclei,heterochromatin in
R3068	T12851	T12850	_lexicallyChainedTo	cells,pluripotent
R4193	T14740	T14739	_lexicallyChainedTo	embryos,Generation of
R320	T2100	T2099	_lexicallyChainedTo	H4,histone
R1	T648	T647	_lexicallyChainedTo	Cells,Pluripotent
R2	T671	T670	_lexicallyChainedTo	stages,two-cell
R3	T678	T677	_lexicallyChainedTo	cell stage,two-
R4	T702	T701	_lexicallyChainedTo	cells,pluripotent

PMC:1630711 JSONTXT

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craft-sa-dev

craft-ca-core-ex-dev

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craft-ca-core-dev

PMC:1630711 JSON TXT