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PMC:1626108 / 49213-50132 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T9875 0-11 NNS denotes Homogenates
T9876 79-87 VBN denotes prepared
T9877 12-15 CC denotes and
T9878 16-25 NN denotes detergent
T9879 26-37 VBN denotes solubilized
T9880 38-46 NNS denotes extracts
T9881 47-49 IN denotes of
T9882 50-57 NNS denotes tissues
T9883 58-61 CC denotes and
T9884 62-73 NNS denotes immunoblots
T9885 74-78 VBD denotes were
T9886 88-90 IN denotes as
T9887 91-100 VBN denotes described
T9888 101-110 RB denotes elsewhere
T9889 111-112 -LRB- denotes [
T9890 112-114 CD denotes 69
T9891 114-115 -RRB- denotes ]
T9892 115-116 . denotes .
T9893 116-314 sentence denotes Quantitation of immunoblot bands calibrated against internal marker bands, such as mHsp70 and Nup153, was carried out with Gel Pro Analyzer (MediaCybernetics, San Diego, California, United States).
T9894 117-129 NN denotes Quantitation
T9895 223-230 VBN denotes carried
T9896 130-132 IN denotes of
T9897 133-143 NN denotes immunoblot
T9898 144-149 NNS denotes bands
T9899 150-160 VBN denotes calibrated
T9900 161-168 IN denotes against
T9901 169-177 JJ denotes internal
T9902 185-190 NNS denotes bands
T9903 178-184 NN denotes marker
T9904 190-192 , denotes ,
T9905 192-196 JJ denotes such
T9906 197-199 IN denotes as
T9907 200-206 NN denotes mHsp70
T9908 207-210 CC denotes and
T9909 211-217 NN denotes Nup153
T9910 217-219 , denotes ,
T9911 219-222 VBD denotes was
T9912 231-234 RP denotes out
T9913 235-239 IN denotes with
T9914 240-243 NNP denotes Gel
T9915 248-256 NNP denotes Analyzer
T9916 244-247 NNP denotes Pro
T9917 257-258 -LRB- denotes (
T9918 258-274 NNPS denotes MediaCybernetics
T9919 274-276 , denotes ,
T9920 276-279 NNP denotes San
T9921 280-285 NNP denotes Diego
T9922 285-287 , denotes ,
T9923 287-297 NNP denotes California
T9924 297-299 , denotes ,
T9925 299-305 NNP denotes United
T9926 306-312 NNP denotes States
T9927 312-313 -RRB- denotes )
T9928 313-314 . denotes .
T9929 314-530 sentence denotes For determination of ATP levels, freshly dissected and flash-frozen tissues were homogenized in 2.5% trichloroacetic acid (TCA), neutralized, and diluted to a final concentration of 0.1% with Tris-Acetate (pH 7.75).
T9930 315-318 IN denotes For
T9931 396-407 VBN denotes homogenized
T9932 319-332 NN denotes determination
T9933 333-335 IN denotes of
T9934 336-339 NN denotes ATP
T9935 340-346 NNS denotes levels
T9936 346-348 , denotes ,
T9937 348-355 RB denotes freshly
T9938 356-365 VBN denotes dissected
T9939 383-390 NNS denotes tissues
T9940 366-369 CC denotes and
T9941 370-375 NN denotes flash
T9942 376-382 VBN denotes frozen
T9943 375-376 HYPH denotes -
T9944 391-395 VBD denotes were
T9945 408-410 IN denotes in
T9946 411-414 CD denotes 2.5
T9947 414-415 NN denotes %
T9948 416-431 JJ denotes trichloroacetic
T9949 432-436 NN denotes acid
T9950 437-438 -LRB- denotes (
T9951 438-441 NN denotes TCA
T9952 441-442 -RRB- denotes )
T9953 442-444 , denotes ,
T9954 444-455 VBN denotes neutralized
T9955 455-457 , denotes ,
T9956 457-460 CC denotes and
T9957 461-468 VBD denotes diluted
T9958 469-471 IN denotes to
T9959 472-473 DT denotes a
T9960 480-493 NN denotes concentration
T9961 474-479 JJ denotes final
T9962 494-496 IN denotes of
T9963 497-500 CD denotes 0.1
T9964 500-501 NN denotes %
T9965 502-506 IN denotes with
T9966 507-511 NN denotes Tris
T9967 512-519 NN denotes Acetate
T9968 511-512 HYPH denotes -
T9969 520-521 -LRB- denotes (
T9970 521-523 NN denotes pH
T9971 524-528 CD denotes 7.75
T9972 528-529 -RRB- denotes )
T9973 529-530 . denotes .
T9974 530-795 sentence denotes ATP measurements were carried out with the ENLITEN ATP Assay System Bioluminescence Detection Kit for ATP (Promega, Madison, Wisconsin, United States) as per the manufacturer's instruction in SpectraMax-M5 (Molecular Devices, Sunnyvale, California, United States).
T9975 531-534 NN denotes ATP
T9976 535-547 NNS denotes measurements
T9977 553-560 VBN denotes carried
T9978 548-552 VBD denotes were
T9979 561-564 RP denotes out
T9980 565-569 IN denotes with
T9981 570-573 DT denotes the
T9982 625-628 NNP denotes Kit
T9983 574-581 NNP denotes ENLITEN
T9984 582-585 NNP denotes ATP
T9985 592-598 NNP denotes System
T9986 586-591 NNP denotes Assay
T9987 599-614 NNP denotes Bioluminescence
T9988 615-624 NNP denotes Detection
T9989 629-632 IN denotes for
T9990 633-636 NN denotes ATP
T9991 637-638 -LRB- denotes (
T9992 638-645 NNP denotes Promega
T9993 645-647 , denotes ,
T9994 647-654 NNP denotes Madison
T9995 654-656 , denotes ,
T9996 656-665 NNP denotes Wisconsin
T9997 665-667 , denotes ,
T9998 667-673 NNP denotes United
T9999 674-680 NNP denotes States
T10000 680-681 -RRB- denotes )
T10001 682-684 IN denotes as
T10002 685-688 IN denotes per
T10003 689-692 DT denotes the
T10004 693-705 NN denotes manufacturer
T10005 708-719 NN denotes instruction
T10006 705-707 POS denotes 's
T10007 720-722 IN denotes in
T10008 723-733 NNP denotes SpectraMax
T10009 734-736 NNP denotes M5
T10010 733-734 HYPH denotes -
T10011 737-738 -LRB- denotes (
T10012 748-755 NNPS denotes Devices
T10013 738-747 NNP denotes Molecular
T10014 755-757 , denotes ,
T10015 757-766 NNP denotes Sunnyvale
T10016 766-768 , denotes ,
T10017 768-778 NNP denotes California
T10018 778-780 , denotes ,
T10019 780-786 NNP denotes United
T10020 787-793 NNP denotes States
T10021 793-794 -RRB- denotes )
T10022 794-795 . denotes .
T10023 795-919 sentence denotes Protein concentration was measured by the BCA method (BioRad, Hercules, California, United States) using BSA as a standard.
T10024 796-803 NN denotes Protein
T10025 804-817 NN denotes concentration
T10026 822-830 VBN denotes measured
T10027 818-821 VBD denotes was
T10028 831-833 IN denotes by
T10029 834-837 DT denotes the
T10030 842-848 NN denotes method
T10031 838-841 NN denotes BCA
T10032 849-850 -LRB- denotes (
T10033 850-856 NNP denotes BioRad
T10034 856-858 , denotes ,
T10035 858-866 NNP denotes Hercules
T10036 866-868 , denotes ,
T10037 868-878 NNP denotes California
T10038 878-880 , denotes ,
T10039 880-886 NNP denotes United
T10040 887-893 NNP denotes States
T10041 893-894 -RRB- denotes )
T10042 895-900 VBG denotes using
T10043 901-904 NN denotes BSA
T10044 905-907 IN denotes as
T10045 908-909 DT denotes a
T10046 910-918 NN denotes standard
T10047 918-919 . denotes .
R6597 T9875 T9876 nsubjpass Homogenates,prepared
R6598 T9877 T9875 cc and,Homogenates
R6599 T9878 T9879 npadvmod detergent,solubilized
R6600 T9879 T9880 amod solubilized,extracts
R6601 T9880 T9875 conj extracts,Homogenates
R6602 T9881 T9875 prep of,Homogenates
R6603 T9882 T9881 pobj tissues,of
R6604 T9883 T9882 cc and,tissues
R6605 T9884 T9882 conj immunoblots,tissues
R6606 T9885 T9876 auxpass were,prepared
R6607 T9886 T9887 mark as,described
R6608 T9887 T9876 advcl described,prepared
R6609 T9888 T9887 advmod elsewhere,described
R6610 T9889 T9890 punct [,69
R6611 T9890 T9887 parataxis 69,described
R6612 T9891 T9890 punct ],69
R6613 T9892 T9876 punct .,prepared
R6614 T9894 T9895 nsubjpass Quantitation,carried
R6615 T9896 T9894 prep of,Quantitation
R6616 T9897 T9898 compound immunoblot,bands
R6617 T9898 T9896 pobj bands,of
R6618 T9899 T9894 acl calibrated,Quantitation
R6619 T9900 T9899 prep against,calibrated
R6620 T9901 T9902 amod internal,bands
R6621 T9902 T9900 pobj bands,against
R6622 T9903 T9902 compound marker,bands
R6623 T9904 T9902 punct ", ",bands
R6624 T9905 T9906 amod such,as
R6625 T9906 T9902 prep as,bands
R6626 T9907 T9906 pobj mHsp70,as
R6627 T9908 T9907 cc and,mHsp70
R6628 T9909 T9907 conj Nup153,mHsp70
R6629 T9910 T9895 punct ", ",carried
R6630 T9911 T9895 auxpass was,carried
R6631 T9912 T9895 prt out,carried
R6632 T9913 T9895 prep with,carried
R6633 T9914 T9915 compound Gel,Analyzer
R6634 T9915 T9913 pobj Analyzer,with
R6635 T9916 T9915 compound Pro,Analyzer
R6636 T9917 T9918 punct (,MediaCybernetics
R6637 T9918 T9915 parataxis MediaCybernetics,Analyzer
R6638 T9919 T9918 punct ", ",MediaCybernetics
R6639 T9920 T9921 compound San,Diego
R6640 T9921 T9918 npadvmod Diego,MediaCybernetics
R6641 T9922 T9918 punct ", ",MediaCybernetics
R6642 T9923 T9918 npadvmod California,MediaCybernetics
R6643 T9924 T9918 punct ", ",MediaCybernetics
R6644 T9925 T9926 compound United,States
R6645 T9926 T9918 npadvmod States,MediaCybernetics
R6646 T9927 T9918 punct ),MediaCybernetics
R6647 T9928 T9895 punct .,carried
R6648 T9930 T9931 prep For,homogenized
R6649 T9932 T9930 pobj determination,For
R6650 T9933 T9932 prep of,determination
R6651 T9934 T9935 compound ATP,levels
R6652 T9935 T9933 pobj levels,of
R6653 T9936 T9931 punct ", ",homogenized
R6654 T9937 T9938 advmod freshly,dissected
R6655 T9938 T9939 amod dissected,tissues
R6656 T9939 T9931 nsubjpass tissues,homogenized
R6657 T9940 T9938 cc and,dissected
R6658 T9941 T9942 npadvmod flash,frozen
R6659 T9942 T9938 conj frozen,dissected
R6660 T9943 T9942 punct -,frozen
R6661 T9944 T9931 auxpass were,homogenized
R6662 T9945 T9931 prep in,homogenized
R6663 T9946 T9947 nummod 2.5,%
R6664 T9947 T9945 pobj %,in
R6665 T9948 T9949 amod trichloroacetic,acid
R6666 T9949 T9947 appos acid,%
R6667 T9950 T9949 punct (,acid
R6668 T9951 T9949 appos TCA,acid
R6669 T9952 T9931 punct ),homogenized
R6670 T9953 T9931 punct ", ",homogenized
R6671 T9954 T9931 conj neutralized,homogenized
R6672 T9955 T9954 punct ", ",neutralized
R6673 T9956 T9954 cc and,neutralized
R6674 T9957 T9954 conj diluted,neutralized
R6675 T9958 T9957 prep to,diluted
R6676 T9959 T9960 det a,concentration
R6677 T9960 T9958 pobj concentration,to
R6678 T9961 T9960 amod final,concentration
R6679 T9962 T9960 prep of,concentration
R6680 T9963 T9964 nummod 0.1,%
R6681 T9964 T9962 pobj %,of
R6682 T9965 T9957 prep with,diluted
R6683 T9966 T9967 compound Tris,Acetate
R6684 T9967 T9965 pobj Acetate,with
R6685 T9968 T9967 punct -,Acetate
R6686 T9969 T9970 punct (,pH
R6687 T9970 T9967 parataxis pH,Acetate
R6688 T9971 T9970 nummod 7.75,pH
R6689 T9972 T9970 punct ),pH
R6690 T9973 T9931 punct .,homogenized
R6691 T9975 T9976 compound ATP,measurements
R6692 T9976 T9977 nsubjpass measurements,carried
R6693 T9978 T9977 auxpass were,carried
R6694 T9979 T9977 prt out,carried
R6695 T9980 T9977 prep with,carried
R6696 T9981 T9982 det the,Kit
R6697 T9982 T9980 pobj Kit,with
R6698 T9983 T9982 compound ENLITEN,Kit
R6699 T9984 T9985 compound ATP,System
R6700 T9985 T9982 compound System,Kit
R6701 T9986 T9985 compound Assay,System
R6702 T9987 T9988 compound Bioluminescence,Detection
R6703 T9988 T9982 compound Detection,Kit
R6704 T9989 T9982 prep for,Kit
R6705 T9990 T9989 pobj ATP,for
R6706 T9991 T9992 punct (,Promega
R6707 T9992 T9982 parataxis Promega,Kit
R6708 T9993 T9992 punct ", ",Promega
R6709 T9994 T9992 npadvmod Madison,Promega
R6710 T9995 T9992 punct ", ",Promega
R6711 T9996 T9992 npadvmod Wisconsin,Promega
R6712 T9997 T9992 punct ", ",Promega
R6713 T9998 T9999 compound United,States
R6714 T9999 T9992 npadvmod States,Promega
R6715 T10000 T9992 punct ),Promega
R6716 T10001 T9977 prep as,carried
R6717 T10002 T10001 prep per,as
R6718 T10003 T10004 det the,manufacturer
R6719 T10004 T10005 poss manufacturer,instruction
R6720 T10005 T10002 pobj instruction,per
R6721 T10006 T10004 case 's,manufacturer
R6722 T10007 T9977 prep in,carried
R6723 T10008 T10009 compound SpectraMax,M5
R6724 T10009 T10007 pobj M5,in
R6725 T10010 T10009 punct -,M5
R6726 T10011 T10012 punct (,Devices
R6727 T10012 T10009 parataxis Devices,M5
R6728 T10013 T10012 compound Molecular,Devices
R6729 T10014 T10012 punct ", ",Devices
R6730 T10015 T10012 npadvmod Sunnyvale,Devices
R6731 T10016 T10012 punct ", ",Devices
R6732 T10017 T10012 npadvmod California,Devices
R6733 T10018 T10012 punct ", ",Devices
R6734 T10019 T10020 compound United,States
R6735 T10020 T10012 npadvmod States,Devices
R6736 T10021 T10012 punct ),Devices
R6737 T10022 T9977 punct .,carried
R6738 T10024 T10025 compound Protein,concentration
R6739 T10025 T10026 nsubjpass concentration,measured
R6740 T10027 T10026 auxpass was,measured
R6741 T10028 T10026 prep by,measured
R6742 T10029 T10030 det the,method
R6743 T10030 T10028 pobj method,by
R6744 T10031 T10030 compound BCA,method
R6745 T10032 T10033 punct (,BioRad
R6746 T10033 T10030 parataxis BioRad,method
R6747 T10034 T10033 punct ", ",BioRad
R6748 T10035 T10033 npadvmod Hercules,BioRad
R6749 T10036 T10033 punct ", ",BioRad
R6750 T10037 T10033 npadvmod California,BioRad
R6751 T10038 T10033 punct ", ",BioRad
R6752 T10039 T10040 compound United,States
R6753 T10040 T10033 npadvmod States,BioRad
R6754 T10041 T10033 punct ),BioRad
R6755 T10042 T10026 advcl using,measured
R6756 T10043 T10042 dobj BSA,using
R6757 T10044 T10042 prep as,using
R6758 T10045 T10046 det a,standard
R6759 T10046 T10044 pobj standard,as
R6760 T10047 T10026 punct .,measured

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T9847 16-25 CHEBI_EXT:27780 denotes detergent
T9848 50-57 UBERON:0000479 denotes tissues
T9849 178-184 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T9850 211-217 PR_EXT:000011508 denotes Nup153
T9851 336-339 CHEBI_EXT:ATP denotes ATP
T9852 383-390 UBERON:0000479 denotes tissues
T9853 416-436 CHEBI:30956 denotes trichloroacetic acid
T9854 438-441 CHEBI:30956 denotes TCA
T9855 507-519 CHEBI:66869 denotes Tris-Acetate
T9856 531-534 CHEBI_EXT:ATP denotes ATP
T9857 582-585 CHEBI_EXT:ATP denotes ATP
T9858 599-614 GO:0008218 denotes Bioluminescence
T9859 633-636 CHEBI_EXT:ATP denotes ATP
T9860 738-747 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T9861 796-803 CHEBI_PR_EXT:protein denotes Protein

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T9836 16-25 CHEBI:27780 denotes detergent
T9837 50-57 UBERON:0000479 denotes tissues
T9838 211-217 PR:000011508 denotes Nup153
T9839 383-390 UBERON:0000479 denotes tissues
T9840 416-436 CHEBI:30956 denotes trichloroacetic acid
T9841 438-441 CHEBI:30956 denotes TCA
T9842 507-519 CHEBI:66869 denotes Tris-Acetate
T9843 599-614 GO:0008218 denotes Bioluminescence

2_test

Id Subject Object Predicate Lexical cue
17069463-12874105-85858888 112-114 12874105 denotes 69
T9054 112-114 12874105 denotes 69