Id |
Subject |
Object |
Predicate |
Lexical cue |
T7095 |
10-19 |
NN |
denotes |
Targeting |
T7096 |
20-22 |
IN |
denotes |
of |
T7097 |
23-26 |
DT |
denotes |
the |
T7098 |
37-41 |
NN |
denotes |
Gene |
T7099 |
27-32 |
NN |
denotes |
Mouse |
T7100 |
33-36 |
NN |
denotes |
Xpd |
T7101 |
41-162 |
sentence |
denotes |
(A) Schematic representation of the genomic structure and partial restriction map of the wt and targeted mouse Xpd loci. |
T7102 |
42-43 |
-LRB- |
denotes |
( |
T7103 |
43-44 |
LS |
denotes |
A |
T7104 |
56-70 |
NN |
denotes |
representation |
T7105 |
44-45 |
-RRB- |
denotes |
) |
T7106 |
46-55 |
JJ |
denotes |
Schematic |
T7107 |
71-73 |
IN |
denotes |
of |
T7108 |
74-77 |
DT |
denotes |
the |
T7109 |
86-95 |
NN |
denotes |
structure |
T7110 |
78-85 |
JJ |
denotes |
genomic |
T7111 |
96-99 |
CC |
denotes |
and |
T7112 |
100-107 |
JJ |
denotes |
partial |
T7113 |
120-123 |
NN |
denotes |
map |
T7114 |
108-119 |
NN |
denotes |
restriction |
T7115 |
124-126 |
IN |
denotes |
of |
T7116 |
127-130 |
DT |
denotes |
the |
T7117 |
157-161 |
NNS |
denotes |
loci |
T7118 |
131-133 |
NN |
denotes |
wt |
T7119 |
134-137 |
CC |
denotes |
and |
T7120 |
138-146 |
VBN |
denotes |
targeted |
T7121 |
147-152 |
NN |
denotes |
mouse |
T7122 |
153-156 |
NN |
denotes |
Xpd |
T7123 |
161-162 |
. |
denotes |
. |
T7124 |
162-287 |
sentence |
denotes |
For the wt Xpd allele, shaded boxes represent coding regions of exons 12 and 19–23; the 3′UTR is represented by an open box. |
T7125 |
163-166 |
IN |
denotes |
For |
T7126 |
260-271 |
VBN |
denotes |
represented |
T7127 |
167-170 |
DT |
denotes |
the |
T7128 |
178-184 |
NN |
denotes |
allele |
T7129 |
171-173 |
NN |
denotes |
wt |
T7130 |
174-177 |
NN |
denotes |
Xpd |
T7131 |
184-186 |
, |
denotes |
, |
T7132 |
186-192 |
JJ |
denotes |
shaded |
T7133 |
193-198 |
NNS |
denotes |
boxes |
T7134 |
199-208 |
VBP |
denotes |
represent |
T7135 |
209-215 |
VBG |
denotes |
coding |
T7136 |
216-223 |
NNS |
denotes |
regions |
T7137 |
224-226 |
IN |
denotes |
of |
T7138 |
227-232 |
NNS |
denotes |
exons |
T7139 |
233-235 |
CD |
denotes |
12 |
T7140 |
236-239 |
CC |
denotes |
and |
T7141 |
240-242 |
CD |
denotes |
19 |
T7142 |
242-243 |
SYM |
denotes |
– |
T7143 |
243-245 |
CD |
denotes |
23 |
T7144 |
245-246 |
: |
denotes |
; |
T7145 |
247-250 |
DT |
denotes |
the |
T7146 |
253-256 |
NN |
denotes |
UTR |
T7147 |
251-252 |
CD |
denotes |
3 |
T7148 |
252-253 |
SYM |
denotes |
′ |
T7149 |
257-259 |
VBZ |
denotes |
is |
T7150 |
272-274 |
IN |
denotes |
by |
T7151 |
275-277 |
DT |
denotes |
an |
T7152 |
283-286 |
NN |
denotes |
box |
T7153 |
278-282 |
JJ |
denotes |
open |
T7154 |
286-287 |
. |
denotes |
. |
T7155 |
287-375 |
sentence |
denotes |
TGA indicates the translational stop codon; PolyA indicates the polyadenylation signal. |
T7156 |
288-291 |
NN |
denotes |
TGA |
T7157 |
292-301 |
VBZ |
denotes |
indicates |
T7158 |
338-347 |
VBZ |
denotes |
indicates |
T7159 |
302-305 |
DT |
denotes |
the |
T7160 |
325-330 |
NN |
denotes |
codon |
T7161 |
306-319 |
JJ |
denotes |
translational |
T7162 |
320-324 |
NN |
denotes |
stop |
T7163 |
330-331 |
: |
denotes |
; |
T7164 |
332-337 |
NN |
denotes |
PolyA |
T7165 |
348-351 |
DT |
denotes |
the |
T7166 |
368-374 |
NN |
denotes |
signal |
T7167 |
352-367 |
NN |
denotes |
polyadenylation |
T7168 |
374-375 |
. |
denotes |
. |
T7169 |
375-567 |
sentence |
denotes |
For the XpdTTD targeted allele, the 194–base pair (bp) human XPD cDNA fragment fused to exon 22 is indicated as a striped box including the TTD (R722W) mutation indicated by a vertical arrow. |
T7170 |
376-379 |
IN |
denotes |
For |
T7171 |
475-484 |
VBN |
denotes |
indicated |
T7172 |
380-383 |
DT |
denotes |
the |
T7173 |
400-406 |
NN |
denotes |
allele |
T7174 |
384-390 |
NN |
denotes |
XpdTTD |
T7175 |
391-399 |
VBN |
denotes |
targeted |
T7176 |
406-408 |
, |
denotes |
, |
T7177 |
408-411 |
DT |
denotes |
the |
T7178 |
446-454 |
NN |
denotes |
fragment |
T7179 |
412-415 |
CD |
denotes |
194 |
T7180 |
421-425 |
NN |
denotes |
pair |
T7181 |
415-416 |
HYPH |
denotes |
– |
T7182 |
416-420 |
NN |
denotes |
base |
T7183 |
426-427 |
-LRB- |
denotes |
( |
T7184 |
427-429 |
NN |
denotes |
bp |
T7185 |
429-430 |
-RRB- |
denotes |
) |
T7186 |
431-436 |
JJ |
denotes |
human |
T7187 |
437-440 |
NN |
denotes |
XPD |
T7188 |
441-445 |
NN |
denotes |
cDNA |
T7189 |
455-460 |
VBN |
denotes |
fused |
T7190 |
461-463 |
IN |
denotes |
to |
T7191 |
464-468 |
NN |
denotes |
exon |
T7192 |
469-471 |
CD |
denotes |
22 |
T7193 |
472-474 |
VBZ |
denotes |
is |
T7194 |
485-487 |
IN |
denotes |
as |
T7195 |
488-489 |
DT |
denotes |
a |
T7196 |
498-501 |
NN |
denotes |
box |
T7197 |
490-497 |
VBN |
denotes |
striped |
T7198 |
502-511 |
VBG |
denotes |
including |
T7199 |
512-515 |
DT |
denotes |
the |
T7200 |
528-536 |
NN |
denotes |
mutation |
T7201 |
516-519 |
NN |
denotes |
TTD |
T7202 |
520-521 |
-LRB- |
denotes |
( |
T7203 |
521-526 |
NN |
denotes |
R722W |
T7204 |
526-527 |
-RRB- |
denotes |
) |
T7205 |
537-546 |
VBN |
denotes |
indicated |
T7206 |
547-549 |
IN |
denotes |
by |
T7207 |
550-551 |
DT |
denotes |
a |
T7208 |
561-566 |
NN |
denotes |
arrow |
T7209 |
552-560 |
JJ |
denotes |
vertical |
T7210 |
566-567 |
. |
denotes |
. |
T7211 |
567-739 |
sentence |
denotes |
Chicken β-globin exons 2 and 3 including the 3′UTR are indicated as black boxes with corresponding Roman numerals followed by the β-globin polyadenylation signal (PolyA*). |
T7212 |
568-575 |
NN |
denotes |
Chicken |
T7213 |
591-592 |
CD |
denotes |
2 |
T7214 |
576-577 |
NN |
denotes |
β |
T7215 |
578-584 |
NN |
denotes |
globin |
T7216 |
577-578 |
HYPH |
denotes |
- |
T7217 |
585-590 |
NNS |
denotes |
exons |
T7218 |
623-632 |
VBN |
denotes |
indicated |
T7219 |
593-596 |
CC |
denotes |
and |
T7220 |
597-598 |
CD |
denotes |
3 |
T7221 |
599-608 |
VBG |
denotes |
including |
T7222 |
609-612 |
DT |
denotes |
the |
T7223 |
615-618 |
NN |
denotes |
UTR |
T7224 |
613-614 |
CD |
denotes |
3 |
T7225 |
614-615 |
HYPH |
denotes |
′ |
T7226 |
619-622 |
VBP |
denotes |
are |
T7227 |
633-635 |
IN |
denotes |
as |
T7228 |
636-641 |
JJ |
denotes |
black |
T7229 |
642-647 |
NNS |
denotes |
boxes |
T7230 |
648-652 |
IN |
denotes |
with |
T7231 |
653-666 |
VBG |
denotes |
corresponding |
T7232 |
673-681 |
NNS |
denotes |
numerals |
T7233 |
667-672 |
NNP |
denotes |
Roman |
T7234 |
682-690 |
VBN |
denotes |
followed |
T7235 |
691-693 |
IN |
denotes |
by |
T7236 |
694-697 |
DT |
denotes |
the |
T7237 |
723-729 |
NN |
denotes |
signal |
T7238 |
698-699 |
NN |
denotes |
β |
T7239 |
700-706 |
NN |
denotes |
globin |
T7240 |
699-700 |
HYPH |
denotes |
- |
T7241 |
707-722 |
NN |
denotes |
polyadenylation |
T7242 |
730-731 |
-LRB- |
denotes |
( |
T7243 |
731-737 |
NN |
denotes |
PolyA* |
T7244 |
737-738 |
-RRB- |
denotes |
) |
T7245 |
738-739 |
. |
denotes |
. |
T7246 |
739-900 |
sentence |
denotes |
For the Xpd†XP and Xpd†XPCS targeted alleles, vertical arrows indicate XPCS (G602D-encoding) and XP (R683W-encoding) mutations in exons 19 and 22, respectively. |
T7247 |
740-743 |
IN |
denotes |
For |
T7248 |
802-810 |
VBP |
denotes |
indicate |
T7249 |
744-747 |
DT |
denotes |
the |
T7250 |
777-784 |
NNS |
denotes |
alleles |
T7251 |
748-754 |
NN |
denotes |
Xpd†XP |
T7252 |
755-758 |
CC |
denotes |
and |
T7253 |
759-767 |
NN |
denotes |
Xpd†XPCS |
T7254 |
768-776 |
VBN |
denotes |
targeted |
T7255 |
784-786 |
, |
denotes |
, |
T7256 |
786-794 |
JJ |
denotes |
vertical |
T7257 |
795-801 |
NNS |
denotes |
arrows |
T7258 |
811-815 |
NN |
denotes |
XPCS |
T7259 |
857-866 |
NNS |
denotes |
mutations |
T7260 |
816-817 |
-LRB- |
denotes |
( |
T7261 |
823-831 |
VBG |
denotes |
encoding |
T7262 |
817-822 |
NN |
denotes |
G602D |
T7263 |
822-823 |
HYPH |
denotes |
- |
T7264 |
831-832 |
-RRB- |
denotes |
) |
T7265 |
833-836 |
CC |
denotes |
and |
T7266 |
837-839 |
NN |
denotes |
XP |
T7267 |
840-841 |
-LRB- |
denotes |
( |
T7268 |
847-855 |
VBG |
denotes |
encoding |
T7269 |
841-846 |
NN |
denotes |
R683W |
T7270 |
846-847 |
HYPH |
denotes |
- |
T7271 |
855-856 |
-RRB- |
denotes |
) |
T7272 |
867-869 |
IN |
denotes |
in |
T7273 |
870-875 |
NNS |
denotes |
exons |
T7274 |
876-878 |
CD |
denotes |
19 |
T7275 |
879-882 |
CC |
denotes |
and |
T7276 |
883-885 |
CD |
denotes |
22 |
T7277 |
885-887 |
, |
denotes |
, |
T7278 |
887-899 |
RB |
denotes |
respectively |
T7279 |
899-900 |
. |
denotes |
. |
T7280 |
900-993 |
sentence |
denotes |
The unique 3′ probe located outside the targeting construct is marked by a thick black line. |
T7281 |
901-904 |
DT |
denotes |
The |
T7282 |
915-920 |
NN |
denotes |
probe |
T7283 |
905-911 |
JJ |
denotes |
unique |
T7284 |
912-913 |
CD |
denotes |
3 |
T7285 |
913-914 |
SYM |
denotes |
′ |
T7286 |
964-970 |
VBN |
denotes |
marked |
T7287 |
921-928 |
VBN |
denotes |
located |
T7288 |
929-936 |
IN |
denotes |
outside |
T7289 |
937-940 |
DT |
denotes |
the |
T7290 |
951-960 |
NN |
denotes |
construct |
T7291 |
941-950 |
VBG |
denotes |
targeting |
T7292 |
961-963 |
VBZ |
denotes |
is |
T7293 |
971-973 |
IN |
denotes |
by |
T7294 |
974-975 |
DT |
denotes |
a |
T7295 |
988-992 |
NN |
denotes |
line |
T7296 |
976-981 |
JJ |
denotes |
thick |
T7297 |
982-987 |
JJ |
denotes |
black |
T7298 |
992-993 |
. |
denotes |
. |
T7299 |
993-1073 |
sentence |
denotes |
Restriction sites: B, BamHI; C, ClaI; E, EcoRI; H, HindIII; Hp, HpaI; Sf, SfiI. |
T7300 |
994-1005 |
NN |
denotes |
Restriction |
T7301 |
1006-1011 |
NNS |
denotes |
sites |
T7302 |
1011-1013 |
: |
denotes |
: |
T7303 |
1013-1014 |
NN |
denotes |
B |
T7304 |
1014-1016 |
, |
denotes |
, |
T7305 |
1016-1021 |
NN |
denotes |
BamHI |
T7306 |
1021-1022 |
: |
denotes |
; |
T7307 |
1023-1024 |
NN |
denotes |
C |
T7308 |
1024-1026 |
, |
denotes |
, |
T7309 |
1026-1030 |
NN |
denotes |
ClaI |
T7310 |
1030-1031 |
: |
denotes |
; |
T7311 |
1032-1033 |
NN |
denotes |
E |
T7312 |
1033-1035 |
, |
denotes |
, |
T7313 |
1035-1040 |
NN |
denotes |
EcoRI |
T7314 |
1040-1041 |
: |
denotes |
; |
T7315 |
1042-1043 |
NN |
denotes |
H |
T7316 |
1043-1045 |
, |
denotes |
, |
T7317 |
1045-1052 |
NN |
denotes |
HindIII |
T7318 |
1052-1053 |
: |
denotes |
; |
T7319 |
1054-1056 |
NN |
denotes |
Hp |
T7320 |
1056-1058 |
, |
denotes |
, |
T7321 |
1058-1062 |
NN |
denotes |
HpaI |
T7322 |
1062-1063 |
: |
denotes |
; |
T7323 |
1064-1066 |
NN |
denotes |
Sf |
T7324 |
1066-1068 |
, |
denotes |
, |
T7325 |
1068-1072 |
NN |
denotes |
SfiI |
T7326 |
1072-1073 |
. |
denotes |
. |
T7327 |
1073-1251 |
sentence |
denotes |
(B) Southern blot analysis of EcoRI-digested genomic DNA from wt, Xpd†XPCS/wt, and Xpd†XP/wt recombinant embryonic stem cell clones hybridised with the 3′ probe depicted in (A). |
T7328 |
1074-1075 |
-LRB- |
denotes |
( |
T7329 |
1075-1076 |
LS |
denotes |
B |
T7330 |
1092-1100 |
NN |
denotes |
analysis |
T7331 |
1076-1077 |
-RRB- |
denotes |
) |
T7332 |
1078-1086 |
NNP |
denotes |
Southern |
T7333 |
1087-1091 |
NN |
denotes |
blot |
T7334 |
1101-1103 |
IN |
denotes |
of |
T7335 |
1104-1109 |
NN |
denotes |
EcoRI |
T7336 |
1110-1118 |
VBN |
denotes |
digested |
T7337 |
1109-1110 |
HYPH |
denotes |
- |
T7338 |
1127-1130 |
NN |
denotes |
DNA |
T7339 |
1119-1126 |
JJ |
denotes |
genomic |
T7340 |
1131-1135 |
IN |
denotes |
from |
T7341 |
1136-1138 |
NN |
denotes |
wt |
T7342 |
1199-1205 |
NNS |
denotes |
clones |
T7343 |
1138-1140 |
, |
denotes |
, |
T7344 |
1140-1148 |
NN |
denotes |
Xpd†XPCS |
T7345 |
1149-1151 |
NN |
denotes |
wt |
T7346 |
1148-1149 |
HYPH |
denotes |
/ |
T7347 |
1151-1153 |
, |
denotes |
, |
T7348 |
1153-1156 |
CC |
denotes |
and |
T7349 |
1157-1163 |
NN |
denotes |
Xpd†XP |
T7350 |
1164-1166 |
NN |
denotes |
wt |
T7351 |
1163-1164 |
HYPH |
denotes |
/ |
T7352 |
1167-1178 |
JJ |
denotes |
recombinant |
T7353 |
1179-1188 |
JJ |
denotes |
embryonic |
T7354 |
1194-1198 |
NN |
denotes |
cell |
T7355 |
1189-1193 |
NN |
denotes |
stem |
T7356 |
1206-1216 |
VBN |
denotes |
hybridised |
T7357 |
1217-1221 |
IN |
denotes |
with |
T7358 |
1222-1225 |
DT |
denotes |
the |
T7359 |
1229-1234 |
NN |
denotes |
probe |
T7360 |
1226-1227 |
CD |
denotes |
3 |
T7361 |
1227-1228 |
SYM |
denotes |
′ |
T7362 |
1235-1243 |
VBN |
denotes |
depicted |
T7363 |
1244-1246 |
IN |
denotes |
in |
T7364 |
1247-1248 |
-LRB- |
denotes |
( |
T7365 |
1248-1249 |
NN |
denotes |
A |
T7366 |
1249-1250 |
-RRB- |
denotes |
) |
T7367 |
1250-1251 |
. |
denotes |
. |
T7368 |
1251-1377 |
sentence |
denotes |
The wt allele yields a 6.5-kilobase (kb) fragment, whereas both targeted Xpd†XP and Xpd†XPCS alleles yield a 5.1-kb fragment. |
T7369 |
1252-1255 |
DT |
denotes |
The |
T7370 |
1259-1265 |
NN |
denotes |
allele |
T7371 |
1256-1258 |
NN |
denotes |
wt |
T7372 |
1266-1272 |
VBZ |
denotes |
yields |
T7373 |
1273-1274 |
DT |
denotes |
a |
T7374 |
1293-1301 |
NN |
denotes |
fragment |
T7375 |
1275-1278 |
CD |
denotes |
6.5 |
T7376 |
1279-1287 |
NN |
denotes |
kilobase |
T7377 |
1278-1279 |
HYPH |
denotes |
- |
T7378 |
1288-1289 |
-LRB- |
denotes |
( |
T7379 |
1289-1291 |
NN |
denotes |
kb |
T7380 |
1291-1292 |
-RRB- |
denotes |
) |
T7381 |
1301-1303 |
, |
denotes |
, |
T7382 |
1303-1310 |
IN |
denotes |
whereas |
T7383 |
1353-1358 |
VBP |
denotes |
yield |
T7384 |
1311-1315 |
CC |
denotes |
both |
T7385 |
1345-1352 |
NNS |
denotes |
alleles |
T7386 |
1316-1324 |
VBN |
denotes |
targeted |
T7387 |
1325-1331 |
NN |
denotes |
Xpd†XP |
T7388 |
1332-1335 |
CC |
denotes |
and |
T7389 |
1336-1344 |
NN |
denotes |
Xpd†XPCS |
T7390 |
1359-1360 |
DT |
denotes |
a |
T7391 |
1368-1376 |
NN |
denotes |
fragment |
T7392 |
1361-1364 |
CD |
denotes |
5.1 |
T7393 |
1365-1367 |
NN |
denotes |
kb |
T7394 |
1364-1365 |
HYPH |
denotes |
- |
T7395 |
1376-1377 |
. |
denotes |
. |
T7396 |
1377-1528 |
sentence |
denotes |
(C) Genotyping of wt and targeted alleles by PCR using primers F2, R1, and mR as indicated in (A) yields fragments of 399 bp and 468 bp, respectively. |
T7397 |
1378-1379 |
-LRB- |
denotes |
( |
T7398 |
1379-1380 |
LS |
denotes |
C |
T7399 |
1382-1392 |
NN |
denotes |
Genotyping |
T7400 |
1380-1381 |
-RRB- |
denotes |
) |
T7401 |
1393-1395 |
IN |
denotes |
of |
T7402 |
1396-1398 |
NN |
denotes |
wt |
T7403 |
1412-1419 |
NNS |
denotes |
alleles |
T7404 |
1399-1402 |
CC |
denotes |
and |
T7405 |
1403-1411 |
VBN |
denotes |
targeted |
T7406 |
1420-1422 |
IN |
denotes |
by |
T7407 |
1423-1426 |
NN |
denotes |
PCR |
T7408 |
1427-1432 |
VBG |
denotes |
using |
T7409 |
1433-1440 |
NNS |
denotes |
primers |
T7410 |
1441-1443 |
NN |
denotes |
F2 |
T7411 |
1443-1445 |
, |
denotes |
, |
T7412 |
1445-1447 |
NN |
denotes |
R1 |
T7413 |
1447-1449 |
, |
denotes |
, |
T7414 |
1449-1452 |
CC |
denotes |
and |
T7415 |
1453-1455 |
NN |
denotes |
mR |
T7416 |
1456-1458 |
IN |
denotes |
as |
T7417 |
1459-1468 |
VBN |
denotes |
indicated |
T7418 |
1469-1471 |
IN |
denotes |
in |
T7419 |
1472-1473 |
-LRB- |
denotes |
( |
T7420 |
1473-1474 |
LS |
denotes |
A |
T7421 |
1483-1492 |
NNS |
denotes |
fragments |
T7422 |
1474-1475 |
-RRB- |
denotes |
) |
T7423 |
1476-1482 |
VBZ |
denotes |
yields |
T7424 |
1493-1495 |
IN |
denotes |
of |
T7425 |
1496-1499 |
CD |
denotes |
399 |
T7426 |
1500-1502 |
NN |
denotes |
bp |
T7427 |
1503-1506 |
CC |
denotes |
and |
T7428 |
1507-1510 |
CD |
denotes |
468 |
T7429 |
1511-1513 |
NN |
denotes |
bp |
T7430 |
1513-1515 |
, |
denotes |
, |
T7431 |
1515-1527 |
RB |
denotes |
respectively |
T7432 |
1527-1528 |
. |
denotes |
. |
T7433 |
1528-1772 |
sentence |
denotes |
(D) RT-PCR detection of mRNA expression originating from the targeted †XP and †XPCS alleles in embryonic stem cell clones using primers F1 (hybridising outside the targeting construct) and mR as indicated in (A) results in a 1,416-bp fragment. |
T7434 |
1529-1530 |
-LRB- |
denotes |
( |
T7435 |
1530-1531 |
LS |
denotes |
D |
T7436 |
1741-1748 |
VBZ |
denotes |
results |
T7437 |
1531-1532 |
-RRB- |
denotes |
) |
T7438 |
1533-1535 |
NN |
denotes |
RT |
T7439 |
1536-1539 |
NN |
denotes |
PCR |
T7440 |
1535-1536 |
HYPH |
denotes |
- |
T7441 |
1540-1549 |
NN |
denotes |
detection |
T7442 |
1550-1552 |
IN |
denotes |
of |
T7443 |
1553-1557 |
NN |
denotes |
mRNA |
T7444 |
1558-1568 |
NN |
denotes |
expression |
T7445 |
1569-1580 |
VBG |
denotes |
originating |
T7446 |
1581-1585 |
IN |
denotes |
from |
T7447 |
1586-1589 |
DT |
denotes |
the |
T7448 |
1613-1620 |
NNS |
denotes |
alleles |
T7449 |
1590-1598 |
VBN |
denotes |
targeted |
T7450 |
1599-1602 |
NN |
denotes |
†XP |
T7451 |
1603-1606 |
CC |
denotes |
and |
T7452 |
1607-1612 |
NN |
denotes |
†XPCS |
T7453 |
1621-1623 |
IN |
denotes |
in |
T7454 |
1624-1633 |
JJ |
denotes |
embryonic |
T7455 |
1639-1643 |
NN |
denotes |
cell |
T7456 |
1634-1638 |
NN |
denotes |
stem |
T7457 |
1644-1650 |
NNS |
denotes |
clones |
T7458 |
1651-1656 |
VBG |
denotes |
using |
T7459 |
1657-1664 |
NNS |
denotes |
primers |
T7460 |
1665-1667 |
NN |
denotes |
F1 |
T7461 |
1668-1669 |
-LRB- |
denotes |
( |
T7462 |
1669-1680 |
VBG |
denotes |
hybridising |
T7463 |
1681-1688 |
IN |
denotes |
outside |
T7464 |
1689-1692 |
DT |
denotes |
the |
T7465 |
1703-1712 |
NN |
denotes |
construct |
T7466 |
1693-1702 |
VBG |
denotes |
targeting |
T7467 |
1712-1713 |
-RRB- |
denotes |
) |
T7468 |
1714-1717 |
CC |
denotes |
and |
T7469 |
1718-1720 |
NN |
denotes |
mR |
T7470 |
1721-1723 |
IN |
denotes |
as |
T7471 |
1724-1733 |
VBN |
denotes |
indicated |
T7472 |
1734-1736 |
IN |
denotes |
in |
T7473 |
1737-1738 |
-LRB- |
denotes |
( |
T7474 |
1738-1739 |
NN |
denotes |
A |
T7475 |
1739-1740 |
-RRB- |
denotes |
) |
T7476 |
1749-1751 |
IN |
denotes |
in |
T7477 |
1752-1753 |
DT |
denotes |
a |
T7478 |
1763-1771 |
NN |
denotes |
fragment |
T7479 |
1754-1759 |
CD |
denotes |
1,416 |
T7480 |
1760-1762 |
NN |
denotes |
bp |
T7481 |
1759-1760 |
HYPH |
denotes |
- |
T7482 |
1771-1772 |
. |
denotes |
. |
T7483 |
1772-1964 |
sentence |
denotes |
(E) Northern blot analysis of total RNA isolated from testis of homozygous wt and XpdTTD/TTD, heterozygous Xpd†XPCS/wt and XpdTTD/wt, and compound heterozygous Xpd†XPCS/TTD mice as indicated. |
T7484 |
1773-1774 |
-LRB- |
denotes |
( |
T7485 |
1774-1775 |
LS |
denotes |
E |
T7486 |
1791-1799 |
NN |
denotes |
analysis |
T7487 |
1775-1776 |
-RRB- |
denotes |
) |
T7488 |
1777-1785 |
NNP |
denotes |
Northern |
T7489 |
1786-1790 |
NN |
denotes |
blot |
T7490 |
1800-1802 |
IN |
denotes |
of |
T7491 |
1803-1808 |
JJ |
denotes |
total |
T7492 |
1809-1812 |
NN |
denotes |
RNA |
T7493 |
1813-1821 |
VBN |
denotes |
isolated |
T7494 |
1822-1826 |
IN |
denotes |
from |
T7495 |
1827-1833 |
NN |
denotes |
testis |
T7496 |
1834-1836 |
IN |
denotes |
of |
T7497 |
1837-1847 |
JJ |
denotes |
homozygous |
T7498 |
1848-1850 |
NN |
denotes |
wt |
T7499 |
1946-1950 |
NNS |
denotes |
mice |
T7500 |
1851-1854 |
CC |
denotes |
and |
T7501 |
1855-1861 |
NN |
denotes |
XpdTTD |
T7502 |
1862-1865 |
NN |
denotes |
TTD |
T7503 |
1861-1862 |
HYPH |
denotes |
/ |
T7504 |
1865-1867 |
, |
denotes |
, |
T7505 |
1867-1879 |
JJ |
denotes |
heterozygous |
T7506 |
1889-1891 |
NN |
denotes |
wt |
T7507 |
1880-1888 |
NN |
denotes |
Xpd†XPCS |
T7508 |
1888-1889 |
HYPH |
denotes |
/ |
T7509 |
1892-1895 |
CC |
denotes |
and |
T7510 |
1896-1902 |
NN |
denotes |
XpdTTD |
T7511 |
1903-1905 |
NN |
denotes |
wt |
T7512 |
1902-1903 |
HYPH |
denotes |
/ |
T7513 |
1905-1907 |
, |
denotes |
, |
T7514 |
1907-1910 |
CC |
denotes |
and |
T7515 |
1911-1919 |
JJ |
denotes |
compound |
T7516 |
1942-1945 |
NN |
denotes |
TTD |
T7517 |
1920-1932 |
JJ |
denotes |
heterozygous |
T7518 |
1933-1941 |
NN |
denotes |
Xpd†XPCS |
T7519 |
1941-1942 |
HYPH |
denotes |
/ |
T7520 |
1951-1953 |
IN |
denotes |
as |
T7521 |
1954-1963 |
VBN |
denotes |
indicated |
T7522 |
1963-1964 |
. |
denotes |
. |
T7523 |
1964-2102 |
sentence |
denotes |
Hybridisation with a 1.4-kb mouse Xpd cDNA probe detects mRNAs of 4, 3.3, and 2.7 kb from wt, Xpd†XPCS, and XpdTTD alleles, respectively. |
T7524 |
1965-1978 |
NN |
denotes |
Hybridisation |
T7525 |
2014-2021 |
VBZ |
denotes |
detects |
T7526 |
1979-1983 |
IN |
denotes |
with |
T7527 |
1984-1985 |
DT |
denotes |
a |
T7528 |
2008-2013 |
NN |
denotes |
probe |
T7529 |
1986-1989 |
CD |
denotes |
1.4 |
T7530 |
1990-1992 |
NN |
denotes |
kb |
T7531 |
1989-1990 |
HYPH |
denotes |
- |
T7532 |
1993-1998 |
NN |
denotes |
mouse |
T7533 |
1999-2002 |
NN |
denotes |
Xpd |
T7534 |
2003-2007 |
NN |
denotes |
cDNA |
T7535 |
2022-2027 |
NNS |
denotes |
mRNAs |
T7536 |
2028-2030 |
IN |
denotes |
of |
T7537 |
2031-2032 |
CD |
denotes |
4 |
T7538 |
2047-2049 |
NN |
denotes |
kb |
T7539 |
2032-2034 |
, |
denotes |
, |
T7540 |
2034-2037 |
CD |
denotes |
3.3 |
T7541 |
2037-2039 |
, |
denotes |
, |
T7542 |
2039-2042 |
CC |
denotes |
and |
T7543 |
2043-2046 |
CD |
denotes |
2.7 |
T7544 |
2050-2054 |
IN |
denotes |
from |
T7545 |
2055-2057 |
NN |
denotes |
wt |
T7546 |
2080-2087 |
NNS |
denotes |
alleles |
T7547 |
2057-2059 |
, |
denotes |
, |
T7548 |
2059-2067 |
NN |
denotes |
Xpd†XPCS |
T7549 |
2067-2069 |
, |
denotes |
, |
T7550 |
2069-2072 |
CC |
denotes |
and |
T7551 |
2073-2079 |
NN |
denotes |
XpdTTD |
T7552 |
2087-2089 |
, |
denotes |
, |
T7553 |
2089-2101 |
RB |
denotes |
respectively |
T7554 |
2101-2102 |
. |
denotes |
. |
T7555 |
2102-2196 |
sentence |
denotes |
An ethidium bromide (EtBr)–stained gel showing the amount of total RNA loaded is shown below. |
T7556 |
2103-2105 |
DT |
denotes |
An |
T7557 |
2138-2141 |
NN |
denotes |
gel |
T7558 |
2106-2114 |
NN |
denotes |
ethidium |
T7559 |
2115-2122 |
NN |
denotes |
bromide |
T7560 |
2130-2137 |
VBN |
denotes |
stained |
T7561 |
2123-2124 |
-LRB- |
denotes |
( |
T7562 |
2124-2128 |
NN |
denotes |
EtBr |
T7563 |
2128-2129 |
-RRB- |
denotes |
) |
T7564 |
2129-2130 |
HYPH |
denotes |
– |
T7565 |
2184-2189 |
VBN |
denotes |
shown |
T7566 |
2142-2149 |
VBG |
denotes |
showing |
T7567 |
2150-2153 |
DT |
denotes |
the |
T7568 |
2154-2160 |
NN |
denotes |
amount |
T7569 |
2161-2163 |
IN |
denotes |
of |
T7570 |
2164-2169 |
JJ |
denotes |
total |
T7571 |
2170-2173 |
NN |
denotes |
RNA |
T7572 |
2174-2180 |
VBN |
denotes |
loaded |
T7573 |
2181-2183 |
VBZ |
denotes |
is |
T7574 |
2190-2195 |
RB |
denotes |
below |
T7575 |
2195-2196 |
. |
denotes |
. |
R4726 |
T7096 |
T7095 |
prep |
of,Targeting |
R4727 |
T7097 |
T7098 |
det |
the,Gene |
R4728 |
T7098 |
T7096 |
pobj |
Gene,of |
R4729 |
T7099 |
T7098 |
compound |
Mouse,Gene |
R4730 |
T7100 |
T7098 |
compound |
Xpd,Gene |
R4731 |
T7102 |
T7103 |
punct |
(,A |
R4732 |
T7103 |
T7104 |
meta |
A,representation |
R4733 |
T7105 |
T7103 |
punct |
),A |
R4734 |
T7106 |
T7104 |
amod |
Schematic,representation |
R4735 |
T7107 |
T7104 |
prep |
of,representation |
R4736 |
T7108 |
T7109 |
det |
the,structure |
R4737 |
T7109 |
T7107 |
pobj |
structure,of |
R4738 |
T7110 |
T7109 |
amod |
genomic,structure |
R4739 |
T7111 |
T7104 |
cc |
and,representation |
R4740 |
T7112 |
T7113 |
amod |
partial,map |
R4741 |
T7113 |
T7104 |
conj |
map,representation |
R4742 |
T7114 |
T7113 |
compound |
restriction,map |
R4743 |
T7115 |
T7113 |
prep |
of,map |
R4744 |
T7116 |
T7117 |
det |
the,loci |
R4745 |
T7117 |
T7115 |
pobj |
loci,of |
R4746 |
T7118 |
T7117 |
nmod |
wt,loci |
R4747 |
T7119 |
T7118 |
cc |
and,wt |
R4748 |
T7120 |
T7118 |
conj |
targeted,wt |
R4749 |
T7121 |
T7117 |
compound |
mouse,loci |
R4750 |
T7122 |
T7117 |
compound |
Xpd,loci |
R4751 |
T7123 |
T7113 |
punct |
.,map |
R4752 |
T7125 |
T7126 |
prep |
For,represented |
R4753 |
T7127 |
T7128 |
det |
the,allele |
R4754 |
T7128 |
T7125 |
pobj |
allele,For |
R4755 |
T7129 |
T7128 |
compound |
wt,allele |
R4756 |
T7130 |
T7128 |
compound |
Xpd,allele |
R4757 |
T7131 |
T7126 |
punct |
", ",represented |
R4758 |
T7132 |
T7133 |
amod |
shaded,boxes |
R4759 |
T7133 |
T7134 |
nsubj |
boxes,represent |
R4760 |
T7134 |
T7126 |
ccomp |
represent,represented |
R4761 |
T7135 |
T7136 |
amod |
coding,regions |
R4762 |
T7136 |
T7134 |
dobj |
regions,represent |
R4763 |
T7137 |
T7136 |
prep |
of,regions |
R4764 |
T7138 |
T7139 |
nmod |
exons,12 |
R4765 |
T7139 |
T7137 |
pobj |
12,of |
R4766 |
T7140 |
T7139 |
cc |
and,12 |
R4767 |
T7141 |
T7139 |
conj |
19,12 |
R4768 |
T7142 |
T7143 |
punct |
–,23 |
R4769 |
T7143 |
T7141 |
prep |
23,19 |
R4770 |
T7144 |
T7126 |
punct |
;,represented |
R4771 |
T7145 |
T7146 |
det |
the,UTR |
R4772 |
T7146 |
T7126 |
nsubjpass |
UTR,represented |
R4773 |
T7147 |
T7146 |
nummod |
3,UTR |
R4774 |
T7148 |
T7147 |
punct |
′,3 |
R4775 |
T7149 |
T7126 |
auxpass |
is,represented |
R4776 |
T7150 |
T7126 |
agent |
by,represented |
R4777 |
T7151 |
T7152 |
det |
an,box |
R4778 |
T7152 |
T7150 |
pobj |
box,by |
R4779 |
T7153 |
T7152 |
amod |
open,box |
R4780 |
T7154 |
T7126 |
punct |
.,represented |
R4781 |
T7156 |
T7157 |
nsubj |
TGA,indicates |
R4782 |
T7157 |
T7158 |
ccomp |
indicates,indicates |
R4783 |
T7159 |
T7160 |
det |
the,codon |
R4784 |
T7160 |
T7157 |
dobj |
codon,indicates |
R4785 |
T7161 |
T7160 |
amod |
translational,codon |
R4786 |
T7162 |
T7160 |
compound |
stop,codon |
R4787 |
T7163 |
T7158 |
punct |
;,indicates |
R4788 |
T7164 |
T7158 |
nsubj |
PolyA,indicates |
R4789 |
T7165 |
T7166 |
det |
the,signal |
R4790 |
T7166 |
T7158 |
dobj |
signal,indicates |
R4791 |
T7167 |
T7166 |
compound |
polyadenylation,signal |
R4792 |
T7168 |
T7158 |
punct |
.,indicates |
R4793 |
T7170 |
T7171 |
prep |
For,indicated |
R4794 |
T7172 |
T7173 |
det |
the,allele |
R4795 |
T7173 |
T7170 |
pobj |
allele,For |
R4796 |
T7174 |
T7173 |
nmod |
XpdTTD,allele |
R4797 |
T7175 |
T7173 |
amod |
targeted,allele |
R4798 |
T7176 |
T7171 |
punct |
", ",indicated |
R4799 |
T7177 |
T7178 |
det |
the,fragment |
R4800 |
T7178 |
T7171 |
nsubjpass |
fragment,indicated |
R4801 |
T7179 |
T7180 |
nummod |
194,pair |
R4802 |
T7180 |
T7178 |
nmod |
pair,fragment |
R4803 |
T7181 |
T7180 |
punct |
–,pair |
R4804 |
T7182 |
T7180 |
nmod |
base,pair |
R4805 |
T7183 |
T7180 |
punct |
(,pair |
R4806 |
T7184 |
T7180 |
appos |
bp,pair |
R4807 |
T7185 |
T7178 |
punct |
),fragment |
R4808 |
T7186 |
T7178 |
amod |
human,fragment |
R4809 |
T7187 |
T7178 |
compound |
XPD,fragment |
R4810 |
T7188 |
T7178 |
compound |
cDNA,fragment |
R4811 |
T7189 |
T7178 |
acl |
fused,fragment |
R4812 |
T7190 |
T7189 |
prep |
to,fused |
R4813 |
T7191 |
T7190 |
pobj |
exon,to |
R4814 |
T7192 |
T7191 |
nummod |
22,exon |
R4815 |
T7193 |
T7171 |
auxpass |
is,indicated |
R4816 |
T7194 |
T7171 |
prep |
as,indicated |
R4817 |
T7195 |
T7196 |
det |
a,box |
R4818 |
T7196 |
T7194 |
pobj |
box,as |
R4819 |
T7197 |
T7196 |
amod |
striped,box |
R4820 |
T7198 |
T7171 |
prep |
including,indicated |
R4821 |
T7199 |
T7200 |
det |
the,mutation |
R4822 |
T7200 |
T7198 |
pobj |
mutation,including |
R4823 |
T7201 |
T7200 |
nmod |
TTD,mutation |
R4824 |
T7202 |
T7203 |
punct |
(,R722W |
R4825 |
T7203 |
T7201 |
parataxis |
R722W,TTD |
R4826 |
T7204 |
T7203 |
punct |
),R722W |
R4827 |
T7205 |
T7200 |
acl |
indicated,mutation |
R4828 |
T7206 |
T7205 |
agent |
by,indicated |
R4829 |
T7207 |
T7208 |
det |
a,arrow |
R4830 |
T7208 |
T7206 |
pobj |
arrow,by |
R4831 |
T7209 |
T7208 |
amod |
vertical,arrow |
R4832 |
T7210 |
T7171 |
punct |
.,indicated |
R4833 |
T7212 |
T7213 |
nmod |
Chicken,2 |
R4834 |
T7213 |
T7218 |
nsubjpass |
2,indicated |
R4835 |
T7214 |
T7215 |
nmod |
β,globin |
R4836 |
T7215 |
T7213 |
nmod |
globin,2 |
R4837 |
T7216 |
T7215 |
punct |
-,globin |
R4838 |
T7217 |
T7213 |
nmod |
exons,2 |
R4839 |
T7219 |
T7213 |
cc |
and,2 |
R4840 |
T7220 |
T7213 |
conj |
3,2 |
R4841 |
T7221 |
T7213 |
prep |
including,2 |
R4842 |
T7222 |
T7223 |
det |
the,UTR |
R4843 |
T7223 |
T7221 |
pobj |
UTR,including |
R4844 |
T7224 |
T7223 |
nummod |
3,UTR |
R4845 |
T7225 |
T7224 |
punct |
′,3 |
R4846 |
T7226 |
T7218 |
auxpass |
are,indicated |
R4847 |
T7227 |
T7218 |
prep |
as,indicated |
R4848 |
T7228 |
T7229 |
amod |
black,boxes |
R4849 |
T7229 |
T7227 |
pobj |
boxes,as |
R4850 |
T7230 |
T7229 |
prep |
with,boxes |
R4851 |
T7231 |
T7232 |
amod |
corresponding,numerals |
R4852 |
T7232 |
T7230 |
pobj |
numerals,with |
R4853 |
T7233 |
T7232 |
compound |
Roman,numerals |
R4854 |
T7234 |
T7232 |
acl |
followed,numerals |
R4855 |
T7235 |
T7234 |
agent |
by,followed |
R4856 |
T7236 |
T7237 |
det |
the,signal |
R4857 |
T7237 |
T7235 |
pobj |
signal,by |
R4858 |
T7238 |
T7239 |
compound |
β,globin |
R4859 |
T7239 |
T7237 |
compound |
globin,signal |
R4860 |
T7240 |
T7239 |
punct |
-,globin |
R4861 |
T7241 |
T7237 |
compound |
polyadenylation,signal |
R4862 |
T7242 |
T7243 |
punct |
(,PolyA* |
R4863 |
T7243 |
T7237 |
parataxis |
PolyA*,signal |
R4864 |
T7244 |
T7243 |
punct |
),PolyA* |
R4865 |
T7245 |
T7218 |
punct |
.,indicated |
R4866 |
T7247 |
T7248 |
prep |
For,indicate |
R4867 |
T7249 |
T7250 |
det |
the,alleles |
R4868 |
T7250 |
T7247 |
pobj |
alleles,For |
R4869 |
T7251 |
T7250 |
nmod |
Xpd†XP,alleles |
R4870 |
T7252 |
T7251 |
cc |
and,Xpd†XP |
R4871 |
T7253 |
T7251 |
conj |
Xpd†XPCS,Xpd†XP |
R4872 |
T7254 |
T7250 |
amod |
targeted,alleles |
R4873 |
T7255 |
T7248 |
punct |
", ",indicate |
R4874 |
T7256 |
T7257 |
amod |
vertical,arrows |
R4875 |
T7257 |
T7248 |
nsubj |
arrows,indicate |
R4876 |
T7258 |
T7259 |
nmod |
XPCS,mutations |
R4877 |
T7259 |
T7248 |
dobj |
mutations,indicate |
R4878 |
T7260 |
T7261 |
punct |
(,encoding |
R4879 |
T7261 |
T7258 |
parataxis |
encoding,XPCS |
R4880 |
T7262 |
T7261 |
npadvmod |
G602D,encoding |
R4881 |
T7263 |
T7261 |
punct |
-,encoding |
R4882 |
T7264 |
T7261 |
punct |
),encoding |
R4883 |
T7265 |
T7258 |
cc |
and,XPCS |
R4884 |
T7266 |
T7258 |
conj |
XP,XPCS |
R4885 |
T7267 |
T7268 |
punct |
(,encoding |
R4886 |
T7268 |
T7266 |
parataxis |
encoding,XP |
R4887 |
T7269 |
T7268 |
npadvmod |
R683W,encoding |
R4888 |
T7270 |
T7268 |
punct |
-,encoding |
R4889 |
T7271 |
T7268 |
punct |
),encoding |
R4890 |
T7272 |
T7259 |
prep |
in,mutations |
R4891 |
T7273 |
T7274 |
nmod |
exons,19 |
R4892 |
T7274 |
T7272 |
pobj |
19,in |
R4893 |
T7275 |
T7274 |
cc |
and,19 |
R4894 |
T7276 |
T7274 |
conj |
22,19 |
R4895 |
T7277 |
T7248 |
punct |
", ",indicate |
R4896 |
T7278 |
T7248 |
advmod |
respectively,indicate |
R4897 |
T7279 |
T7248 |
punct |
.,indicate |
R4898 |
T7281 |
T7282 |
det |
The,probe |
R4899 |
T7282 |
T7286 |
nsubjpass |
probe,marked |
R4900 |
T7283 |
T7282 |
amod |
unique,probe |
R4901 |
T7284 |
T7282 |
nummod |
3,probe |
R4902 |
T7285 |
T7284 |
punct |
′,3 |
R4903 |
T7287 |
T7282 |
acl |
located,probe |
R4904 |
T7288 |
T7287 |
prep |
outside,located |
R4905 |
T7289 |
T7290 |
det |
the,construct |
R4906 |
T7290 |
T7288 |
pobj |
construct,outside |
R4907 |
T7291 |
T7290 |
amod |
targeting,construct |
R4908 |
T7292 |
T7286 |
auxpass |
is,marked |
R4909 |
T7293 |
T7286 |
agent |
by,marked |
R4910 |
T7294 |
T7295 |
det |
a,line |
R4911 |
T7295 |
T7293 |
pobj |
line,by |
R4912 |
T7296 |
T7295 |
amod |
thick,line |
R4913 |
T7297 |
T7295 |
amod |
black,line |
R4914 |
T7298 |
T7286 |
punct |
.,marked |
R4915 |
T7300 |
T7301 |
compound |
Restriction,sites |
R4916 |
T7302 |
T7301 |
punct |
: ,sites |
R4917 |
T7303 |
T7301 |
appos |
B,sites |
R4918 |
T7304 |
T7303 |
punct |
", ",B |
R4919 |
T7305 |
T7303 |
appos |
BamHI,B |
R4920 |
T7306 |
T7303 |
punct |
;,B |
R4921 |
T7307 |
T7303 |
appos |
C,B |
R4922 |
T7308 |
T7307 |
punct |
", ",C |
R4923 |
T7309 |
T7307 |
appos |
ClaI,C |
R4924 |
T7310 |
T7303 |
punct |
;,B |
R4925 |
T7311 |
T7303 |
appos |
E,B |
R4926 |
T7312 |
T7311 |
punct |
", ",E |
R4927 |
T7313 |
T7311 |
appos |
EcoRI,E |
R4928 |
T7314 |
T7303 |
punct |
;,B |
R4929 |
T7315 |
T7303 |
appos |
H,B |
R4930 |
T7316 |
T7315 |
punct |
", ",H |
R4931 |
T7317 |
T7315 |
appos |
HindIII,H |
R4932 |
T7318 |
T7303 |
punct |
;,B |
R4933 |
T7319 |
T7303 |
appos |
Hp,B |
R4934 |
T7320 |
T7319 |
punct |
", ",Hp |
R4935 |
T7321 |
T7319 |
appos |
HpaI,Hp |
R4936 |
T7322 |
T7303 |
punct |
;,B |
R4937 |
T7323 |
T7303 |
appos |
Sf,B |
R4938 |
T7324 |
T7323 |
punct |
", ",Sf |
R4939 |
T7325 |
T7323 |
appos |
SfiI,Sf |
R4940 |
T7326 |
T7301 |
punct |
.,sites |
R4941 |
T7328 |
T7329 |
punct |
(,B |
R4942 |
T7329 |
T7330 |
meta |
B,analysis |
R4943 |
T7331 |
T7329 |
punct |
),B |
R4944 |
T7332 |
T7333 |
compound |
Southern,blot |
R4945 |
T7333 |
T7330 |
compound |
blot,analysis |
R4946 |
T7334 |
T7330 |
prep |
of,analysis |
R4947 |
T7335 |
T7336 |
npadvmod |
EcoRI,digested |
R4948 |
T7336 |
T7338 |
amod |
digested,DNA |
R4949 |
T7337 |
T7336 |
punct |
-,digested |
R4950 |
T7338 |
T7334 |
pobj |
DNA,of |
R4951 |
T7339 |
T7338 |
amod |
genomic,DNA |
R4952 |
T7340 |
T7338 |
prep |
from,DNA |
R4953 |
T7341 |
T7342 |
nmod |
wt,clones |
R4954 |
T7342 |
T7340 |
pobj |
clones,from |
R4955 |
T7343 |
T7341 |
punct |
", ",wt |
R4956 |
T7344 |
T7345 |
compound |
Xpd†XPCS,wt |
R4957 |
T7345 |
T7341 |
conj |
wt,wt |
R4958 |
T7346 |
T7345 |
punct |
/,wt |
R4959 |
T7347 |
T7345 |
punct |
", ",wt |
R4960 |
T7348 |
T7345 |
cc |
and,wt |
R4961 |
T7349 |
T7350 |
compound |
Xpd†XP,wt |
R4962 |
T7350 |
T7345 |
conj |
wt,wt |
R4963 |
T7351 |
T7350 |
punct |
/,wt |
R4964 |
T7352 |
T7342 |
amod |
recombinant,clones |
R4965 |
T7353 |
T7354 |
amod |
embryonic,cell |
R4966 |
T7354 |
T7342 |
compound |
cell,clones |
R4967 |
T7355 |
T7354 |
compound |
stem,cell |
R4968 |
T7356 |
T7342 |
acl |
hybridised,clones |
R4969 |
T7357 |
T7356 |
prep |
with,hybridised |
R4970 |
T7358 |
T7359 |
det |
the,probe |
R4971 |
T7359 |
T7357 |
pobj |
probe,with |
R4972 |
T7360 |
T7359 |
nummod |
3,probe |
R4973 |
T7361 |
T7360 |
punct |
′,3 |
R4974 |
T7362 |
T7359 |
acl |
depicted,probe |
R4975 |
T7363 |
T7362 |
prep |
in,depicted |
R4976 |
T7364 |
T7363 |
punct |
(,in |
R4977 |
T7365 |
T7363 |
pobj |
A,in |
R4978 |
T7366 |
T7330 |
punct |
),analysis |
R4979 |
T7367 |
T7330 |
punct |
.,analysis |
R4980 |
T7369 |
T7370 |
det |
The,allele |
R4981 |
T7370 |
T7372 |
nsubj |
allele,yields |
R4982 |
T7371 |
T7370 |
compound |
wt,allele |
R4983 |
T7373 |
T7374 |
det |
a,fragment |
R4984 |
T7374 |
T7372 |
dobj |
fragment,yields |
R4985 |
T7375 |
T7376 |
nummod |
6.5,kilobase |
R4986 |
T7376 |
T7374 |
nmod |
kilobase,fragment |
R4987 |
T7377 |
T7376 |
punct |
-,kilobase |
R4988 |
T7378 |
T7376 |
punct |
(,kilobase |
R4989 |
T7379 |
T7376 |
appos |
kb,kilobase |
R4990 |
T7380 |
T7374 |
punct |
),fragment |
R4991 |
T7381 |
T7372 |
punct |
", ",yields |
R4992 |
T7382 |
T7383 |
mark |
whereas,yield |
R4993 |
T7383 |
T7372 |
advcl |
yield,yields |
R4994 |
T7384 |
T7385 |
preconj |
both,alleles |
R4995 |
T7385 |
T7383 |
nsubj |
alleles,yield |
R4996 |
T7386 |
T7385 |
amod |
targeted,alleles |
R4997 |
T7387 |
T7385 |
nmod |
Xpd†XP,alleles |
R4998 |
T7388 |
T7387 |
cc |
and,Xpd†XP |
R4999 |
T7389 |
T7387 |
conj |
Xpd†XPCS,Xpd†XP |
R5000 |
T7390 |
T7391 |
det |
a,fragment |
R5001 |
T7391 |
T7383 |
dobj |
fragment,yield |
R5002 |
T7392 |
T7393 |
nummod |
5.1,kb |
R5003 |
T7393 |
T7391 |
compound |
kb,fragment |
R5004 |
T7394 |
T7393 |
punct |
-,kb |
R5005 |
T7395 |
T7372 |
punct |
.,yields |
R5006 |
T7397 |
T7398 |
punct |
(,C |
R5007 |
T7398 |
T7399 |
meta |
C,Genotyping |
R5008 |
T7400 |
T7398 |
punct |
),C |
R5009 |
T7401 |
T7399 |
prep |
of,Genotyping |
R5010 |
T7402 |
T7403 |
nmod |
wt,alleles |
R5011 |
T7403 |
T7401 |
pobj |
alleles,of |
R5012 |
T7404 |
T7402 |
cc |
and,wt |
R5013 |
T7405 |
T7402 |
conj |
targeted,wt |
R5014 |
T7406 |
T7399 |
prep |
by,Genotyping |
R5015 |
T7407 |
T7406 |
pobj |
PCR,by |
R5016 |
T7408 |
T7399 |
acl |
using,Genotyping |
R5017 |
T7409 |
T7408 |
dobj |
primers,using |
R5018 |
T7410 |
T7409 |
appos |
F2,primers |
R5019 |
T7411 |
T7410 |
punct |
", ",F2 |
R5020 |
T7412 |
T7410 |
conj |
R1,F2 |
R5021 |
T7413 |
T7412 |
punct |
", ",R1 |
R5022 |
T7414 |
T7412 |
cc |
and,R1 |
R5023 |
T7415 |
T7412 |
conj |
mR,R1 |
R5024 |
T7416 |
T7417 |
mark |
as,indicated |
R5025 |
T7417 |
T7408 |
advcl |
indicated,using |
R5026 |
T7418 |
T7408 |
prep |
in,using |
R5027 |
T7419 |
T7420 |
punct |
(,A |
R5028 |
T7420 |
T7421 |
meta |
A,fragments |
R5029 |
T7421 |
T7418 |
pobj |
fragments,in |
R5030 |
T7422 |
T7420 |
punct |
),A |
R5031 |
T7423 |
T7421 |
nmod |
yields,fragments |
R5032 |
T7424 |
T7421 |
prep |
of,fragments |
R5033 |
T7425 |
T7426 |
nummod |
399,bp |
R5034 |
T7426 |
T7424 |
pobj |
bp,of |
R5035 |
T7427 |
T7426 |
cc |
and,bp |
R5036 |
T7428 |
T7429 |
nummod |
468,bp |
R5037 |
T7429 |
T7426 |
conj |
bp,bp |
R5038 |
T7430 |
T7408 |
punct |
", ",using |
R5039 |
T7431 |
T7408 |
advmod |
respectively,using |
R5040 |
T7432 |
T7399 |
punct |
.,Genotyping |
R5041 |
T7434 |
T7435 |
punct |
(,D |
R5042 |
T7435 |
T7436 |
meta |
D,results |
R5043 |
T7437 |
T7435 |
punct |
),D |
R5044 |
T7438 |
T7439 |
compound |
RT,PCR |
R5045 |
T7439 |
T7441 |
compound |
PCR,detection |
R5046 |
T7440 |
T7439 |
punct |
-,PCR |
R5047 |
T7441 |
T7436 |
nsubj |
detection,results |
R5048 |
T7442 |
T7441 |
prep |
of,detection |
R5049 |
T7443 |
T7444 |
compound |
mRNA,expression |
R5050 |
T7444 |
T7442 |
pobj |
expression,of |
R5051 |
T7445 |
T7444 |
acl |
originating,expression |
R5052 |
T7446 |
T7445 |
prep |
from,originating |
R5053 |
T7447 |
T7448 |
det |
the,alleles |
R5054 |
T7448 |
T7446 |
pobj |
alleles,from |
R5055 |
T7449 |
T7448 |
amod |
targeted,alleles |
R5056 |
T7450 |
T7448 |
nmod |
†XP,alleles |
R5057 |
T7451 |
T7450 |
cc |
and,†XP |
R5058 |
T7452 |
T7450 |
conj |
†XPCS,†XP |
R5059 |
T7453 |
T7441 |
prep |
in,detection |
R5060 |
T7454 |
T7455 |
amod |
embryonic,cell |
R5061 |
T7455 |
T7457 |
compound |
cell,clones |
R5062 |
T7456 |
T7455 |
compound |
stem,cell |
R5063 |
T7457 |
T7453 |
pobj |
clones,in |
R5064 |
T7458 |
T7441 |
acl |
using,detection |
R5065 |
T7459 |
T7458 |
dobj |
primers,using |
R5066 |
T7460 |
T7459 |
appos |
F1,primers |
R5067 |
T7461 |
T7462 |
punct |
(,hybridising |
R5068 |
T7462 |
T7460 |
parataxis |
hybridising,F1 |
R5069 |
T7463 |
T7462 |
prep |
outside,hybridising |
R5070 |
T7464 |
T7465 |
det |
the,construct |
R5071 |
T7465 |
T7463 |
pobj |
construct,outside |
R5072 |
T7466 |
T7465 |
amod |
targeting,construct |
R5073 |
T7467 |
T7462 |
punct |
),hybridising |
R5074 |
T7468 |
T7460 |
cc |
and,F1 |
R5075 |
T7469 |
T7460 |
conj |
mR,F1 |
R5076 |
T7470 |
T7471 |
mark |
as,indicated |
R5077 |
T7471 |
T7458 |
advcl |
indicated,using |
R5078 |
T7472 |
T7471 |
prep |
in,indicated |
R5079 |
T7473 |
T7472 |
punct |
(,in |
R5080 |
T7474 |
T7472 |
pobj |
A,in |
R5081 |
T7475 |
T7436 |
punct |
),results |
R5082 |
T7476 |
T7436 |
prep |
in,results |
R5083 |
T7477 |
T7478 |
det |
a,fragment |
R5084 |
T7478 |
T7476 |
pobj |
fragment,in |
R5085 |
T7479 |
T7480 |
nummod |
"1,416",bp |
R5086 |
T7480 |
T7478 |
compound |
bp,fragment |
R5087 |
T7481 |
T7480 |
punct |
-,bp |
R5088 |
T7482 |
T7436 |
punct |
.,results |
R5089 |
T7484 |
T7485 |
punct |
(,E |
R5090 |
T7485 |
T7486 |
meta |
E,analysis |
R5091 |
T7487 |
T7485 |
punct |
),E |
R5092 |
T7488 |
T7489 |
compound |
Northern,blot |
R5093 |
T7489 |
T7486 |
compound |
blot,analysis |
R5094 |
T7490 |
T7486 |
prep |
of,analysis |
R5095 |
T7491 |
T7492 |
amod |
total,RNA |
R5096 |
T7492 |
T7490 |
pobj |
RNA,of |
R5097 |
T7493 |
T7492 |
acl |
isolated,RNA |
R5098 |
T7494 |
T7493 |
prep |
from,isolated |
R5099 |
T7495 |
T7494 |
pobj |
testis,from |
R5100 |
T7496 |
T7495 |
prep |
of,testis |
R5101 |
T7497 |
T7498 |
amod |
homozygous,wt |
R5102 |
T7498 |
T7499 |
nmod |
wt,mice |
R5103 |
T7499 |
T7496 |
pobj |
mice,of |
R5104 |
T7500 |
T7498 |
cc |
and,wt |
R5105 |
T7501 |
T7502 |
compound |
XpdTTD,TTD |
R5106 |
T7502 |
T7498 |
conj |
TTD,wt |
R5107 |
T7503 |
T7502 |
punct |
/,TTD |
R5108 |
T7504 |
T7498 |
punct |
", ",wt |
R5109 |
T7505 |
T7506 |
amod |
heterozygous,wt |
R5110 |
T7506 |
T7498 |
conj |
wt,wt |
R5111 |
T7507 |
T7506 |
compound |
Xpd†XPCS,wt |
R5112 |
T7508 |
T7506 |
punct |
/,wt |
R5113 |
T7509 |
T7506 |
cc |
and,wt |
R5114 |
T7510 |
T7511 |
compound |
XpdTTD,wt |
R5115 |
T7511 |
T7506 |
conj |
wt,wt |
R5116 |
T7512 |
T7511 |
punct |
/,wt |
R5117 |
T7513 |
T7506 |
punct |
", ",wt |
R5118 |
T7514 |
T7506 |
cc |
and,wt |
R5119 |
T7515 |
T7516 |
amod |
compound,TTD |
R5120 |
T7516 |
T7506 |
conj |
TTD,wt |
R5121 |
T7517 |
T7516 |
amod |
heterozygous,TTD |
R5122 |
T7518 |
T7516 |
compound |
Xpd†XPCS,TTD |
R5123 |
T7519 |
T7516 |
punct |
/,TTD |
R5124 |
T7520 |
T7521 |
mark |
as,indicated |
R5125 |
T7521 |
T7493 |
advcl |
indicated,isolated |
R5126 |
T7522 |
T7486 |
punct |
.,analysis |
R5127 |
T7524 |
T7525 |
nsubj |
Hybridisation,detects |
R5128 |
T7526 |
T7524 |
prep |
with,Hybridisation |
R5129 |
T7527 |
T7528 |
det |
a,probe |
R5130 |
T7528 |
T7526 |
pobj |
probe,with |
R5131 |
T7529 |
T7530 |
nummod |
1.4,kb |
R5132 |
T7530 |
T7528 |
compound |
kb,probe |
R5133 |
T7531 |
T7530 |
punct |
-,kb |
R5134 |
T7532 |
T7528 |
compound |
mouse,probe |
R5135 |
T7533 |
T7528 |
compound |
Xpd,probe |
R5136 |
T7534 |
T7528 |
compound |
cDNA,probe |
R5137 |
T7535 |
T7525 |
dobj |
mRNAs,detects |
R5138 |
T7536 |
T7535 |
prep |
of,mRNAs |
R5139 |
T7537 |
T7538 |
nummod |
4,kb |
R5140 |
T7538 |
T7536 |
pobj |
kb,of |
R5141 |
T7539 |
T7537 |
punct |
", ",4 |
R5142 |
T7540 |
T7537 |
conj |
3.3,4 |
R5143 |
T7541 |
T7540 |
punct |
", ",3.3 |
R5144 |
T7542 |
T7540 |
cc |
and,3.3 |
R5145 |
T7543 |
T7540 |
conj |
2.7,3.3 |
R5146 |
T7544 |
T7535 |
prep |
from,mRNAs |
R5147 |
T7545 |
T7546 |
nmod |
wt,alleles |
R5148 |
T7546 |
T7544 |
pobj |
alleles,from |
R5149 |
T7547 |
T7545 |
punct |
", ",wt |
R5150 |
T7548 |
T7545 |
conj |
Xpd†XPCS,wt |
R5151 |
T7549 |
T7548 |
punct |
", ",Xpd†XPCS |
R5152 |
T7550 |
T7548 |
cc |
and,Xpd†XPCS |
R5153 |
T7551 |
T7548 |
conj |
XpdTTD,Xpd†XPCS |
R5154 |
T7552 |
T7535 |
punct |
", ",mRNAs |
R5155 |
T7553 |
T7535 |
advmod |
respectively,mRNAs |
R5156 |
T7554 |
T7525 |
punct |
.,detects |
R5157 |
T7556 |
T7557 |
det |
An,gel |
R5158 |
T7557 |
T7565 |
nsubjpass |
gel,shown |
R5159 |
T7558 |
T7559 |
compound |
ethidium,bromide |
R5160 |
T7559 |
T7560 |
npadvmod |
bromide,stained |
R5161 |
T7560 |
T7557 |
amod |
stained,gel |
R5162 |
T7561 |
T7559 |
punct |
(,bromide |
R5163 |
T7562 |
T7559 |
appos |
EtBr,bromide |
R5164 |
T7563 |
T7560 |
punct |
),stained |
R5165 |
T7564 |
T7560 |
punct |
–,stained |
R5166 |
T7566 |
T7557 |
acl |
showing,gel |
R5167 |
T7567 |
T7568 |
det |
the,amount |
R5168 |
T7568 |
T7566 |
dobj |
amount,showing |
R5169 |
T7569 |
T7568 |
prep |
of,amount |
R5170 |
T7570 |
T7571 |
amod |
total,RNA |
R5171 |
T7571 |
T7569 |
pobj |
RNA,of |
R5172 |
T7572 |
T7571 |
acl |
loaded,RNA |
R5173 |
T7573 |
T7565 |
auxpass |
is,shown |
R5174 |
T7574 |
T7565 |
advmod |
below,shown |
R5175 |
T7575 |
T7565 |
punct |
.,shown |