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PMC:1564426 / 6571-11689 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4427 37-41 NCBITaxon:10088 denotes Mice
T4428 69-72 PR_EXT:000004122 denotes Apc
T4429 76-95 GO:0043588 denotes development of skin
T4430 91-95 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T4431 104-114 UBERON:0004529 denotes appendages
T4432 132-136 SO_EXT:0000346 denotes loxP
T4433 175-183 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T4434 191-194 PR_EXT:000004122 denotes Apc
T4435 195-199 SO_EXT:0000704 denotes gene
T4436 203-207 NCBITaxon:10088 denotes mice
T4437 224-233 UBERON:0000922 denotes embryonic
T4438 224-238 _FRAGMENT denotes embryonic stem
T4439 244-249 CL:0002322 denotes cells
T4440 240-242 _FRAGMENT denotes ES
T4441 244-249 CL_GO_EXT:cell denotes cells
T4442 254-258 NCBITaxon:10088 denotes mice
T4443 271-274 PR_EXT:000004122 denotes Apc
T4444 275-281 SO_EXT:0001023 denotes allele
T4445 307-317 SO_EXT:0000346 denotes loxP sites
T4446 318-326 SO:0000357 denotes flanking
T4447 327-330 PR_EXT:000004122 denotes Apc
T4448 331-335 SO_EXT:0000147 denotes exon
T4449 353-356 PR_EXT:P03870 denotes FLP
T4450 353-375 _FRAGMENT denotes FLP recognition target
T4451 382-387 SO_EXT:0000350 denotes sites
T4452 377-380 _FRAGMENT denotes FRT
T4453 388-396 SO:0000357 denotes flanking
T4454 397-400 GO_EXT:phosphoglycerate_kinase denotes PGK
T4455 401-409 CHEBI:7507 denotes neomycin
T4456 420-428 SO_EXT:0005853 denotes cassette
T4457 467-470 PR_EXT:000004122 denotes Apc
T4458 475-481 SO_EXT:0001023 denotes allele
T4459 483-484 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes N
T4460 489-497 CHEBI:7507 denotes neomycin
T4461 498-506 SO_EXT:0005853 denotes cassette
T4462 511-514 GO_EXT:phosphoglycerate_kinase denotes PGK
T4463 515-523 CHEBI:7507 denotes neomycin
T4464 524-532 SO_EXT:0005853 denotes cassette
T4465 537-545 SO_EXT:sequence_insertion_process denotes inserted
T4466 558-573 GO_EXT:transcription denotes transcriptional
T4467 589-592 PR_EXT:000004122 denotes Apc
T4468 596-602 SO_EXT:0000188 denotes intron
T4469 624-628 SO_EXT:0000704 denotes gene
T4470 634-638 _FRAGMENT denotes loxP
T4471 647-652 SO_EXT:0000346 denotes sites
T4472 643-652 SO_EXT:0000350 denotes FRT sites
T4473 685-698 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T4474 712-717 NCBITaxon:10088 denotes mouse
T4475 744-756 SO_EXT:sequence_alteration_entity_or_process denotes modification
T4476 793-795 CL:0002322 denotes ES
T4477 796-802 SO_EXT:sequence_cloned_entity denotes clones
T4478 834-840 GO_PATO_EXT:biological_behavior denotes behave
T4479 864-867 PR_EXT:000004122 denotes Apc
T4480 872-873 SO_EXT:normal_or_wild_type_or_present denotes +
T4481 874-878 NCBITaxon:10088 denotes mice
T4482 922-925 PR_EXT:000004122 denotes Apc
T4483 929-930 SO_EXT:normal_or_wild_type_or_present denotes +
T4484 931-935 NCBITaxon:10088 denotes mice
T4485 973-976 PR_EXT:000004122 denotes Apc
T4486 990-993 PR_EXT:000004122 denotes Apc
T4487 998-1004 SO_EXT:0001023 denotes allele
T4488 1022-1025 GO_EXT:phosphoglycerate_kinase denotes PGK
T4489 1026-1034 CHEBI:7507 denotes neomycin
T4490 1035-1043 SO_EXT:0005853 denotes cassette
T4491 1066-1076 SO_EXT:0000346 denotes loxP sites
T4492 1084-1091 SO_EXT:0000188 denotes introns
T4493 1092-1100 SO:0000357 denotes flanking
T4494 1101-1105 SO_EXT:0000147 denotes exon
T4495 1134-1142 SO_EXT:sequence_deletion_process denotes deleting
T4496 1143-1147 SO_EXT:0000147 denotes exon
T4497 1154-1158 NCBITaxon:10088 denotes mice
T4498 1174-1177 PR_EXT:000004122 denotes Apc
T4499 1181-1182 SO_EXT:normal_or_wild_type_or_present denotes +
T4500 1183-1187 NCBITaxon:10088 denotes mice
T4501 1264-1267 PR_EXT:000004122 denotes Apc
T4502 1280-1283 PR_EXT:000004122 denotes Apc
T4503 1288-1289 SO_EXT:normal_or_wild_type_or_present denotes +
T4504 1295-1301 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4505 1302-1308 SO_EXT:0001023 denotes allele
T4506 1310-1313 PR_EXT:000004122 denotes Apc
T4507 1325-1329 SO_EXT:0000147 denotes exon
T4508 1350-1360 SO_EXT:0000673 denotes transcript
T4509 1374-1378 SO_EXT:0000147 denotes exon
T4510 1395-1403 _FRAGMENT denotes shift in
T4511 1415-1428 GO_SO_EXT:frameshift_entity_or_process denotes reading frame
T4512 1445-1478 SO_EXT:0001587 denotes premature chain termination codon
T4513 1517-1526 SO_EXT:sequence_truncation_process denotes truncated
T4514 1527-1538 CHEBI_SO_EXT:polypeptide denotes polypeptide
T4515 1551-1553 CHEBI_SO_EXT:amino_acid denotes aa
T4516 1588-1590 CHEBI_SO_EXT:amino_acid denotes aa
T4517 1616-1619 PR_EXT:000004122 denotes Apc
T4518 1620-1627 CHEBI_PR_EXT:protein denotes protein
T4519 3220-3224 UBERON:0002415 denotes tail
T4520 3225-3228 CHEBI_SO_EXT:DNA denotes DNA
T4521 3255-3258 PR_EXT:000004122 denotes Apc
T4522 3267-3270 PR_EXT:000004122 denotes Apc
T4523 3320-3328 SO_EXT:sequence_alteration_process denotes modified
T4524 3329-3332 PR_EXT:000004122 denotes Apc
T4525 3333-3339 SO_EXT:0001023 denotes allele
T4526 3353-3357 NCBITaxon:10088 denotes Mice
T4527 3384-3387 PR_EXT:000004122 denotes Apc
T4528 3392-3400 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T4529 3445-3453 UBERON:0000104 denotes lifespan
T4530 3496-3504 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T4531 3508-3512 SO_EXT:0000147 denotes exon
T4532 3574-3577 PR_EXT:000004122 denotes Apc
T4533 3578-3590 SO_EXT:gene_product denotes gene product
T4534 3592-3595 PR_EXT:000004122 denotes Apc
T4535 3600-3601 SO_EXT:normal_or_wild_type_or_present denotes +
T4536 3602-3606 NCBITaxon:10088 denotes mice
T4537 3682-3688 UBERON:0001052 denotes rectal
T4538 3774-3777 PR_EXT:000004122 denotes Apc
T4539 3782-3783 SO_EXT:normal_or_wild_type_or_present denotes +
T4540 3796-3801 NCBITaxon:10088 denotes mouse
T4541 3815-3818 PR_EXT:000004122 denotes Apc
T4542 3823-3824 SO_EXT:normal_or_wild_type_or_present denotes +
T4543 3825-3829 NCBITaxon:10088 denotes mice
T4544 3867-3877 UBERON:0000160 denotes intestinal
T4545 3906-3911 GO:0016265 denotes death
T4546 3941-3950 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4547 3951-3954 PR_EXT:000004122 denotes Apc
T4548 4022-4032 UBERON:0000160 denotes intestinal
T4549 4045-4048 PR_EXT:000004122 denotes Apc
T4550 4053-4054 SO_EXT:normal_or_wild_type_or_present denotes +
T4551 4055-4059 NCBITaxon:10088 denotes mice
T4552 4072-4085 GO_EXT:transcription denotes transcription
T4553 4090-4101 GO:0006412 denotes translation
T4554 4133-4142 SO_EXT:sequence_truncation_process denotes truncated
T4555 4143-4146 PR_EXT:000004122 denotes Apc
T4556 4221-4230 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4557 4231-4234 PR_EXT:000004122 denotes Apc
T4558 4254-4261 SO_EXT:0001023 denotes allelic
T4559 4272-4278 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4560 4279-4285 SO_EXT:0001023 denotes allele
T4561 4331-4335 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T4562 4336-4340 NCBITaxon:10088 denotes mice
T4563 4345-4348 PR_EXT:000004122 denotes Apc
T4564 4353-4354 SO_EXT:normal_or_wild_type_or_present denotes +
T4565 4355-4362 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T4566 4414-4419 GO:0001967 denotes nurse
T4567 4430-4434 NCBITaxon_UBERON_EXT:pup denotes pups
T4568 4466-4469 PR_EXT:000004122 denotes Apc
T4569 4473-4474 SO_EXT:normal_or_wild_type_or_present denotes +
T4570 4475-4479 NCBITaxon:10088 denotes mice
T4571 4510-4513 PR_EXT:000004122 denotes Apc
T4572 4610-4613 PR_EXT:000004122 denotes Apc
T4573 4617-4623 SO_EXT:0001023 denotes allele
T4574 4631-4635 NCBITaxon:10088 denotes mice
T4575 4660-4664 NCBITaxon:10088 denotes mice
T4576 4669-4672 PR_EXT:000004122 denotes Apc
T4577 4676-4682 SO_EXT:0001023 denotes allele
T4578 4743-4752 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4579 4800-4803 PR_EXT:000004122 denotes Apc
T4580 4807-4813 SO_EXT:0001023 denotes allele
T4581 4833-4842 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4582 4843-4849 SO_EXT:0001023 denotes allele
T4583 4866-4869 PR_EXT:000004122 denotes Apc
T4584 4877-4883 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T4585 4889-4892 PR_EXT:000004122 denotes Apc
T4586 4897-4898 SO_EXT:normal_or_wild_type_or_present denotes +
T4587 4899-4903 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T4588 4904-4909 NCBITaxon:10088 denotes mouse
T4589 4925-4928 PR_EXT:000004122 denotes Apc
T4590 4963-4967 GO:0007567 denotes born
T4591 5024-5034 SO_EXT:0000346 denotes loxP sites
T4592 5038-5045 SO_EXT:0000188 denotes introns
T4593 5046-5054 SO:0000357 denotes flanking
T4594 5055-5059 SO_EXT:0000147 denotes exon
T4595 5109-5112 PR_EXT:000004122 denotes Apc
T4596 5113-5117 SO_EXT:0000704 denotes gene
R912 T4439 T4438 _lexicallyChainedTo cells,embryonic stem
R913 T4439 T4440 _lexicallyChainedTo cells,ES
R914 T4451 T4450 _lexicallyChainedTo sites,FLP recognition target
R915 T4451 T4452 _lexicallyChainedTo sites,FRT
R916 T4471 T4470 _lexicallyChainedTo sites,loxP
R917 T4511 T4510 _lexicallyChainedTo reading frame,shift in

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4305 37-41 NCBITaxon:10088 denotes Mice
T4306 69-72 PR:000004122 denotes Apc
T4307 76-95 GO:0043588 denotes development of skin
T4308 104-114 UBERON:0004529 denotes appendages
T4309 132-136 SO:0000346 denotes loxP
T4310 191-194 PR:000004122 denotes Apc
T4311 195-199 SO:0000704 denotes gene
T4312 203-207 NCBITaxon:10088 denotes mice
T4313 224-233 UBERON:0000922 denotes embryonic
T4314 224-238 _FRAGMENT denotes embryonic stem
T4315 244-249 CL:0002322 denotes cells
T4316 240-242 _FRAGMENT denotes ES
T4317 254-258 NCBITaxon:10088 denotes mice
T4318 271-274 PR:000004122 denotes Apc
T4319 275-281 SO:0001023 denotes allele
T4320 307-317 SO:0000346 denotes loxP sites
T4321 318-326 SO:0000357 denotes flanking
T4322 327-330 PR:000004122 denotes Apc
T4323 331-335 SO:0000147 denotes exon
T4324 353-356 PR:P03870 denotes FLP
T4325 353-375 _FRAGMENT denotes FLP recognition target
T4326 382-387 SO:0000350 denotes sites
T4327 377-380 _FRAGMENT denotes FRT
T4328 388-396 SO:0000357 denotes flanking
T4329 401-409 CHEBI:7507 denotes neomycin
T4330 420-428 SO:0005853 denotes cassette
T4331 467-470 PR:000004122 denotes Apc
T4332 475-481 SO:0001023 denotes allele
T4333 489-497 CHEBI:7507 denotes neomycin
T4334 498-506 SO:0005853 denotes cassette
T4335 515-523 CHEBI:7507 denotes neomycin
T4336 524-532 SO:0005853 denotes cassette
T4337 589-592 PR:000004122 denotes Apc
T4338 596-602 SO:0000188 denotes intron
T4339 624-628 SO:0000704 denotes gene
T4340 634-638 _FRAGMENT denotes loxP
T4341 647-652 SO:0000346 denotes sites
T4342 643-652 SO:0000350 denotes FRT sites
T4343 712-717 NCBITaxon:10088 denotes mouse
T4344 793-795 CL:0002322 denotes ES
T4345 864-867 PR:000004122 denotes Apc
T4346 874-878 NCBITaxon:10088 denotes mice
T4347 922-925 PR:000004122 denotes Apc
T4348 931-935 NCBITaxon:10088 denotes mice
T4349 973-976 PR:000004122 denotes Apc
T4350 990-993 PR:000004122 denotes Apc
T4351 998-1004 SO:0001023 denotes allele
T4352 1026-1034 CHEBI:7507 denotes neomycin
T4353 1035-1043 SO:0005853 denotes cassette
T4354 1066-1076 SO:0000346 denotes loxP sites
T4355 1084-1091 SO:0000188 denotes introns
T4356 1092-1100 SO:0000357 denotes flanking
T4357 1101-1105 SO:0000147 denotes exon
T4358 1143-1147 SO:0000147 denotes exon
T4359 1154-1158 NCBITaxon:10088 denotes mice
T4360 1174-1177 PR:000004122 denotes Apc
T4361 1183-1187 NCBITaxon:10088 denotes mice
T4362 1264-1267 PR:000004122 denotes Apc
T4363 1280-1283 PR:000004122 denotes Apc
T4364 1302-1308 SO:0001023 denotes allele
T4365 1310-1313 PR:000004122 denotes Apc
T4366 1325-1329 SO:0000147 denotes exon
T4367 1350-1360 SO:0000673 denotes transcript
T4368 1374-1378 SO:0000147 denotes exon
T4369 1415-1428 SO:0000717 denotes reading frame
T4370 1445-1478 SO:0001587 denotes premature chain termination codon
T4371 1616-1619 PR:000004122 denotes Apc
T4372 3220-3224 UBERON:0002415 denotes tail
T4373 3255-3258 PR:000004122 denotes Apc
T4374 3267-3270 PR:000004122 denotes Apc
T4375 3329-3332 PR:000004122 denotes Apc
T4376 3333-3339 SO:0001023 denotes allele
T4377 3353-3357 NCBITaxon:10088 denotes Mice
T4378 3384-3387 PR:000004122 denotes Apc
T4379 3445-3453 UBERON:0000104 denotes lifespan
T4380 3508-3512 SO:0000147 denotes exon
T4381 3574-3577 PR:000004122 denotes Apc
T4382 3578-3582 SO:0000704 denotes gene
T4383 3592-3595 PR:000004122 denotes Apc
T4384 3602-3606 NCBITaxon:10088 denotes mice
T4385 3682-3688 UBERON:0001052 denotes rectal
T4386 3774-3777 PR:000004122 denotes Apc
T4387 3796-3801 NCBITaxon:10088 denotes mouse
T4388 3815-3818 PR:000004122 denotes Apc
T4389 3825-3829 NCBITaxon:10088 denotes mice
T4390 3867-3877 UBERON:0000160 denotes intestinal
T4391 3906-3911 GO:0016265 denotes death
T4392 3951-3954 PR:000004122 denotes Apc
T4393 4022-4032 UBERON:0000160 denotes intestinal
T4394 4045-4048 PR:000004122 denotes Apc
T4395 4055-4059 NCBITaxon:10088 denotes mice
T4396 4090-4101 GO:0006412 denotes translation
T4397 4143-4146 PR:000004122 denotes Apc
T4398 4231-4234 PR:000004122 denotes Apc
T4399 4254-4261 SO:0001023 denotes allelic
T4400 4279-4285 SO:0001023 denotes allele
T4401 4336-4340 NCBITaxon:10088 denotes mice
T4402 4345-4348 PR:000004122 denotes Apc
T4403 4414-4419 GO:0001967 denotes nurse
T4404 4466-4469 PR:000004122 denotes Apc
T4405 4475-4479 NCBITaxon:10088 denotes mice
T4406 4510-4513 PR:000004122 denotes Apc
T4407 4610-4613 PR:000004122 denotes Apc
T4408 4617-4623 SO:0001023 denotes allele
T4409 4631-4635 NCBITaxon:10088 denotes mice
T4410 4660-4664 NCBITaxon:10088 denotes mice
T4411 4669-4672 PR:000004122 denotes Apc
T4412 4676-4682 SO:0001023 denotes allele
T4413 4800-4803 PR:000004122 denotes Apc
T4414 4807-4813 SO:0001023 denotes allele
T4415 4843-4849 SO:0001023 denotes allele
T4416 4866-4869 PR:000004122 denotes Apc
T4417 4889-4892 PR:000004122 denotes Apc
T4418 4904-4909 NCBITaxon:10088 denotes mouse
T4419 4925-4928 PR:000004122 denotes Apc
T4420 4963-4967 GO:0007567 denotes born
T4421 5024-5034 SO:0000346 denotes loxP sites
T4422 5038-5045 SO:0000188 denotes introns
T4423 5046-5054 SO:0000357 denotes flanking
T4424 5055-5059 SO:0000147 denotes exon
T4425 5109-5112 PR:000004122 denotes Apc
T4426 5113-5117 SO:0000704 denotes gene
R907 T4315 T4314 _lexicallyChainedTo cells,embryonic stem
R908 T4315 T4316 _lexicallyChainedTo cells,ES
R909 T4326 T4325 _lexicallyChainedTo sites,FLP recognition target
R910 T4326 T4327 _lexicallyChainedTo sites,FRT
R911 T4341 T4340 _lexicallyChainedTo sites,loxP

craft-sa-dev

Id Subject Object Predicate Lexical cue
T4597 0-10 NN denotes Generation
T4598 11-13 IN denotes of
T4599 14-17 DT denotes the
T4600 37-41 NNS denotes Mice
T4601 18-24 NN denotes ApcCKO
T4602 25-28 CC denotes and
T4603 29-36 NN denotes ApcΔ580
T4604 41-208 sentence denotes To investigate the role of Apc in development of skin and its appendages, we used the Cre/loxP technology to introduce a conditional mutation of the Apc gene in mice.
T4605 42-44 TO denotes To
T4606 45-56 VB denotes investigate
T4607 119-123 VBD denotes used
T4608 57-60 DT denotes the
T4609 61-65 NN denotes role
T4610 66-68 IN denotes of
T4611 69-72 NN denotes Apc
T4612 73-75 IN denotes in
T4613 76-87 NN denotes development
T4614 88-90 IN denotes of
T4615 91-95 NN denotes skin
T4616 96-99 CC denotes and
T4617 100-103 PRP$ denotes its
T4618 104-114 NNS denotes appendages
T4619 114-116 , denotes ,
T4620 116-118 PRP denotes we
T4621 124-127 DT denotes the
T4622 137-147 NN denotes technology
T4623 128-131 NN denotes Cre
T4624 132-136 NN denotes loxP
T4625 131-132 HYPH denotes /
T4626 148-150 TO denotes to
T4627 151-160 VB denotes introduce
T4628 161-162 DT denotes a
T4629 175-183 NN denotes mutation
T4630 163-174 JJ denotes conditional
T4631 184-186 IN denotes of
T4632 187-190 DT denotes the
T4633 195-199 NN denotes gene
T4634 191-194 NN denotes Apc
T4635 200-202 IN denotes in
T4636 203-207 NNS denotes mice
T4637 207-208 . denotes .
T4638 208-508 sentence denotes We constructed embryonic stem (ES) cells and mice carrying an Apc allele harboring both a pair of loxP sites flanking Apc exon 14 and a pair of FLP recognition target (FRT) sites flanking PGK-neomycin selection cassette by recombineering [12,13] (Figure 1A, ApcCKON allele, N for neomycin cassette).
T4639 209-211 PRP denotes We
T4640 212-223 VBD denotes constructed
T4641 224-233 JJ denotes embryonic
T4642 234-238 NN denotes stem
T4643 244-249 NNS denotes cells
T4644 239-240 -LRB- denotes (
T4645 240-242 NN denotes ES
T4646 242-243 -RRB- denotes )
T4647 250-253 CC denotes and
T4648 254-258 NNS denotes mice
T4649 259-267 VBG denotes carrying
T4650 268-270 DT denotes an
T4651 275-281 NN denotes allele
T4652 271-274 NN denotes Apc
T4653 282-291 VBG denotes harboring
T4654 292-296 CC denotes both
T4655 299-303 NN denotes pair
T4656 297-298 DT denotes a
T4657 304-306 IN denotes of
T4658 307-311 NN denotes loxP
T4659 312-317 NNS denotes sites
T4660 318-326 VBG denotes flanking
T4661 327-330 NN denotes Apc
T4662 331-335 NN denotes exon
T4663 336-338 CD denotes 14
T4664 339-342 CC denotes and
T4665 343-344 DT denotes a
T4666 345-349 NN denotes pair
T4667 350-352 IN denotes of
T4668 353-356 NN denotes FLP
T4669 369-375 NN denotes target
T4670 357-368 NN denotes recognition
T4671 382-387 NNS denotes sites
T4672 376-377 -LRB- denotes (
T4673 377-380 NN denotes FRT
T4674 380-381 -RRB- denotes )
T4675 388-396 VBG denotes flanking
T4676 397-400 NN denotes PGK
T4677 401-409 NN denotes neomycin
T4678 400-401 HYPH denotes -
T4679 420-428 NN denotes cassette
T4680 410-419 NN denotes selection
T4681 429-431 IN denotes by
T4682 432-446 VBG denotes recombineering
T4683 447-448 -LRB- denotes [
T4684 451-453 CD denotes 13
T4685 448-450 CD denotes 12
T4686 450-451 , denotes ,
T4687 453-454 -RRB- denotes ]
T4688 455-456 -LRB- denotes (
T4689 483-484 NN denotes N
T4690 456-462 NN denotes Figure
T4691 463-465 NN denotes 1A
T4692 465-467 , denotes ,
T4693 467-474 NN denotes ApcCKON
T4694 475-481 NN denotes allele
T4695 481-483 , denotes ,
T4696 485-488 IN denotes for
T4697 489-497 NN denotes neomycin
T4698 498-506 NN denotes cassette
T4699 506-507 -RRB- denotes )
T4700 507-508 . denotes .
T4701 508-629 sentence denotes A PGK-neomycin cassette was inserted in the same transcriptional orientation as Apc in intron 14 of the endogenous gene.
T4702 509-510 DT denotes A
T4703 524-532 NN denotes cassette
T4704 511-514 NN denotes PGK
T4705 515-523 NN denotes neomycin
T4706 514-515 HYPH denotes -
T4707 537-545 VBN denotes inserted
T4708 533-536 VBD denotes was
T4709 546-548 IN denotes in
T4710 549-552 DT denotes the
T4711 574-585 NN denotes orientation
T4712 553-557 JJ denotes same
T4713 558-573 JJ denotes transcriptional
T4714 586-588 IN denotes as
T4715 589-592 NN denotes Apc
T4716 593-595 IN denotes in
T4717 596-602 NN denotes intron
T4718 603-605 CD denotes 14
T4719 606-608 IN denotes of
T4720 609-612 DT denotes the
T4721 624-628 NN denotes gene
T4722 613-623 JJ denotes endogenous
T4723 628-629 . denotes .
T4724 629-707 sentence denotes The loxP and FRT sites were used to aid unidirectional recombination [12,13].
T4725 630-633 DT denotes The
T4726 647-652 NNS denotes sites
T4727 634-638 NN denotes loxP
T4728 639-642 CC denotes and
T4729 643-646 NN denotes FRT
T4730 658-662 VBN denotes used
T4731 653-657 VBD denotes were
T4732 663-665 TO denotes to
T4733 666-669 VB denotes aid
T4734 670-684 JJ denotes unidirectional
T4735 685-698 NN denotes recombination
T4736 699-700 -LRB- denotes [
T4737 703-705 CD denotes 13
T4738 700-702 CD denotes 12
T4739 702-703 , denotes ,
T4740 705-706 -RRB- denotes ]
T4741 706-707 . denotes .
T4742 707-857 sentence denotes Two mouse lines containing the same modification were generated from two independent ES clones to ensure that these two lines behave in the same way.
T4743 708-711 CD denotes Two
T4744 718-723 NNS denotes lines
T4745 712-717 NN denotes mouse
T4746 762-771 VBN denotes generated
T4747 724-734 VBG denotes containing
T4748 735-738 DT denotes the
T4749 744-756 NN denotes modification
T4750 739-743 JJ denotes same
T4751 757-761 VBD denotes were
T4752 772-776 IN denotes from
T4753 777-780 CD denotes two
T4754 796-802 NNS denotes clones
T4755 781-792 JJ denotes independent
T4756 793-795 NN denotes ES
T4757 803-805 TO denotes to
T4758 806-812 VB denotes ensure
T4759 813-817 IN denotes that
T4760 834-840 VBP denotes behave
T4761 818-823 DT denotes these
T4762 828-833 NNS denotes lines
T4763 824-827 CD denotes two
T4764 841-843 IN denotes in
T4765 844-847 DT denotes the
T4766 853-856 NN denotes way
T4767 848-852 JJ denotes same
T4768 856-857 . denotes .
T4769 857-1109 sentence denotes These ApcCKON/+ mice were crossed with FLPe-deleter to generate ApcCKO/+ mice that were heterozygous for the final Apc conditional (ApcCKO) allele that removed the PGK-neomycin cassette and contains only the loxP sites in the introns flanking exon 14.
T4770 858-863 DT denotes These
T4771 874-878 NNS denotes mice
T4772 864-871 NN denotes ApcCKON
T4773 871-872 HYPH denotes /
T4774 872-873 SYM denotes +
T4775 884-891 VBN denotes crossed
T4776 879-883 VBD denotes were
T4777 892-896 IN denotes with
T4778 897-901 NN denotes FLPe
T4779 902-909 NN denotes deleter
T4780 901-902 HYPH denotes -
T4781 910-912 TO denotes to
T4782 913-921 VB denotes generate
T4783 922-928 NN denotes ApcCKO
T4784 931-935 NNS denotes mice
T4785 928-929 HYPH denotes /
T4786 929-930 SYM denotes +
T4787 936-940 WDT denotes that
T4788 941-945 VBD denotes were
T4789 946-958 JJ denotes heterozygous
T4790 959-962 IN denotes for
T4791 963-966 DT denotes the
T4792 998-1004 NN denotes allele
T4793 967-972 JJ denotes final
T4794 973-976 NN denotes Apc
T4795 977-988 JJ denotes conditional
T4796 989-990 -LRB- denotes (
T4797 990-996 NN denotes ApcCKO
T4798 996-997 -RRB- denotes )
T4799 1005-1009 WDT denotes that
T4800 1010-1017 VBD denotes removed
T4801 1018-1021 DT denotes the
T4802 1035-1043 NN denotes cassette
T4803 1022-1025 NN denotes PGK
T4804 1026-1034 NN denotes neomycin
T4805 1025-1026 HYPH denotes -
T4806 1044-1047 CC denotes and
T4807 1048-1056 VBZ denotes contains
T4808 1057-1061 RB denotes only
T4809 1071-1076 NNS denotes sites
T4810 1062-1065 DT denotes the
T4811 1066-1070 NN denotes loxP
T4812 1077-1079 IN denotes in
T4813 1080-1083 DT denotes the
T4814 1084-1091 NNS denotes introns
T4815 1092-1100 VBG denotes flanking
T4816 1101-1105 NN denotes exon
T4817 1106-1108 CD denotes 14
T4818 1108-1109 . denotes .
T4819 1109-1290 sentence denotes To assess the effect of deleting exon 14 in mice, both lines of ApcCKO/+ mice were crossed with the Cre-deleter to generate the germline knockout line of Apc, designated ApcΔ580/+.
T4820 1110-1112 TO denotes To
T4821 1113-1119 VB denotes assess
T4822 1193-1200 VBN denotes crossed
T4823 1120-1123 DT denotes the
T4824 1124-1130 NN denotes effect
T4825 1131-1133 IN denotes of
T4826 1134-1142 VBG denotes deleting
T4827 1143-1147 NN denotes exon
T4828 1148-1150 CD denotes 14
T4829 1151-1153 IN denotes in
T4830 1154-1158 NNS denotes mice
T4831 1158-1160 , denotes ,
T4832 1160-1164 DT denotes both
T4833 1165-1170 NNS denotes lines
T4834 1171-1173 IN denotes of
T4835 1174-1180 NN denotes ApcCKO
T4836 1183-1187 NNS denotes mice
T4837 1180-1181 HYPH denotes /
T4838 1181-1182 SYM denotes +
T4839 1188-1192 VBD denotes were
T4840 1201-1205 IN denotes with
T4841 1206-1209 DT denotes the
T4842 1214-1221 NN denotes deleter
T4843 1210-1213 NN denotes Cre
T4844 1213-1214 HYPH denotes -
T4845 1222-1224 TO denotes to
T4846 1225-1233 VB denotes generate
T4847 1234-1237 DT denotes the
T4848 1256-1260 NN denotes line
T4849 1238-1246 NN denotes germline
T4850 1247-1255 NN denotes knockout
T4851 1261-1263 IN denotes of
T4852 1264-1267 NNP denotes Apc
T4853 1267-1269 , denotes ,
T4854 1269-1279 VBN denotes designated
T4855 1280-1287 NN denotes ApcΔ580
T4856 1287-1288 HYPH denotes /
T4857 1288-1289 SYM denotes +
T4858 1289-1290 . denotes .
T4859 1290-1345 sentence denotes The mutant allele (ApcΔ580) lacks exon 14 (Figure 1A).
T4860 1291-1294 DT denotes The
T4861 1302-1308 NN denotes allele
T4862 1295-1301 JJ denotes mutant
T4863 1319-1324 VBZ denotes lacks
T4864 1309-1310 -LRB- denotes (
T4865 1310-1317 NN denotes ApcΔ580
T4866 1317-1318 -RRB- denotes )
T4867 1325-1329 NN denotes exon
T4868 1330-1332 CD denotes 14
T4869 1333-1334 -LRB- denotes (
T4870 1341-1343 NN denotes 1A
T4871 1334-1340 NN denotes Figure
T4872 1343-1344 -RRB- denotes )
T4873 1344-1345 . denotes .
T4874 1345-1628 sentence denotes The transcript from loss of exon 14 results in a shift in the normal reading frame, resulting in a premature chain termination codon which, if utilized, would result in a truncated polypeptide that is 605 aa in length, of which the first 580 aa correspond to the normal Apc protein.
T4875 1346-1349 DT denotes The
T4876 1350-1360 NN denotes transcript
T4877 1382-1389 VBZ denotes results
T4878 1361-1365 IN denotes from
T4879 1366-1370 NN denotes loss
T4880 1371-1373 IN denotes of
T4881 1374-1378 NN denotes exon
T4882 1379-1381 CD denotes 14
T4883 1390-1392 IN denotes in
T4884 1393-1394 DT denotes a
T4885 1395-1400 NN denotes shift
T4886 1401-1403 IN denotes in
T4887 1404-1407 DT denotes the
T4888 1423-1428 NN denotes frame
T4889 1408-1414 JJ denotes normal
T4890 1415-1422 NN denotes reading
T4891 1428-1430 , denotes ,
T4892 1430-1439 VBG denotes resulting
T4893 1440-1442 IN denotes in
T4894 1443-1444 DT denotes a
T4895 1473-1478 NN denotes codon
T4896 1445-1454 JJ denotes premature
T4897 1455-1460 NN denotes chain
T4898 1461-1472 NN denotes termination
T4899 1479-1484 WDT denotes which
T4900 1505-1511 VB denotes result
T4901 1484-1486 , denotes ,
T4902 1486-1488 IN denotes if
T4903 1489-1497 VBN denotes utilized
T4904 1497-1499 , denotes ,
T4905 1499-1504 MD denotes would
T4906 1512-1514 IN denotes in
T4907 1515-1516 DT denotes a
T4908 1527-1538 NN denotes polypeptide
T4909 1517-1526 VBN denotes truncated
T4910 1539-1543 WDT denotes that
T4911 1544-1546 VBZ denotes is
T4912 1547-1550 CD denotes 605
T4913 1551-1553 NNS denotes aa
T4914 1554-1556 IN denotes in
T4915 1557-1563 NN denotes length
T4916 1563-1565 , denotes ,
T4917 1565-1567 IN denotes of
T4918 1591-1601 VBP denotes correspond
T4919 1568-1573 WDT denotes which
T4920 1574-1577 DT denotes the
T4921 1588-1590 NNS denotes aa
T4922 1578-1583 JJ denotes first
T4923 1584-1587 CD denotes 580
T4924 1602-1604 IN denotes to
T4925 1605-1608 DT denotes the
T4926 1620-1627 NN denotes protein
T4927 1609-1615 JJ denotes normal
T4928 1616-1619 NN denotes Apc
T4929 1627-1628 . denotes .
T4930 1628-3352 sentence denotes Figure 1 Generation of the Conditional Apc Allele (A) Schematic diagram of exons 14 and 15 of the mouse Apc gene, the targeting vector, and the resulting conditional allele with 2 LoxP sites sandwiching the exon 14. The PGK-neomycin cassette was inserted within intron 14 by recombineering technique. This cassette is sandwiched by 2 FRT sites that could be removed by crossing to FLPe-expressing mice. Positions of PCR primers used for genotyping PCR (F2, R2, R4) and RT-PCR (F546 and R721) are indicated. Positions of probe used for Southern blot analysis with NdeI sites are also shown. Upon Cre-mediated recombination, exon 14 is removed and leads to truncated Apc protein, of which the first 580 aa correspond to the normal. (B) Southern blot analysis of NdeI-digested genomic tail DNA isolated from F1 mice of various Apc mouse lines (ApcCKON, ApcΔ580), hybridized to a 600-bp probe. Tail genomic DNA from ApcCKON F1 mice derived from a modified ES clone showed a 12-kb band for the ApcCKON allele and a 10-kb band for the wild-type allele, whereas genomic DNA from the ApcΔ580 mouse was heterozygous for the ApcΔ580 allele (9.2-kb band). (C) Kaplan-Meier survival plot of ApcCKO/+ mice (thin solid line, n = 39), ApcCKO/CKO mice (thin dotted line, n = 57), ApcΔ580/+ mice (solid line, n = 51), and wild-type littermates (broken line, n = 21). Heterozygosity of the ApcΔ580 allele led to a significantly shortened survival (p < 0.0001), whereas those of heterozygous and homozygous ApcCKO mice had no significant difference to that of wild-type littermates. Southern blot analysis of tail DNA from F1 offspring of both ApcCKON and ApcΔ580 lines confirmed the germline transmission of modified Apc allele (Figure 1B).
T4931 3194-3202 NNP denotes Southern
T4932 3203-3207 NN denotes blot
T4933 3208-3216 NN denotes analysis
T4934 3281-3290 VBD denotes confirmed
T4935 3217-3219 IN denotes of
T4936 3220-3224 NN denotes tail
T4937 3225-3228 NN denotes DNA
T4938 3229-3233 IN denotes from
T4939 3234-3236 NN denotes F1
T4940 3237-3246 NN denotes offspring
T4941 3247-3249 IN denotes of
T4942 3250-3254 CC denotes both
T4943 3255-3262 NN denotes ApcCKON
T4944 3275-3280 NNS denotes lines
T4945 3263-3266 CC denotes and
T4946 3267-3274 NN denotes ApcΔ580
T4947 3291-3294 DT denotes the
T4948 3304-3316 NN denotes transmission
T4949 3295-3303 NN denotes germline
T4950 3317-3319 IN denotes of
T4951 3320-3328 VBN denotes modified
T4952 3333-3339 NN denotes allele
T4953 3329-3332 NN denotes Apc
T4954 3340-3341 -LRB- denotes (
T4955 3348-3350 NN denotes 1B
T4956 3341-3347 NN denotes Figure
T4957 3350-3351 -RRB- denotes )
T4958 3351-3352 . denotes .
T4959 3352-3466 sentence denotes Mice that are heterozygous for ApcΔ580 mutation are viable but have a significantly reduced lifespan (Figure 1C).
T4960 3353-3357 NNS denotes Mice
T4961 3401-3404 VBP denotes are
T4962 3358-3362 WDT denotes that
T4963 3363-3366 VBP denotes are
T4964 3367-3379 JJ denotes heterozygous
T4965 3380-3383 IN denotes for
T4966 3384-3391 NN denotes ApcΔ580
T4967 3392-3400 NN denotes mutation
T4968 3405-3411 JJ denotes viable
T4969 3412-3415 CC denotes but
T4970 3416-3420 VBP denotes have
T4971 3421-3422 DT denotes a
T4972 3445-3453 NN denotes lifespan
T4973 3423-3436 RB denotes significantly
T4974 3437-3444 VBN denotes reduced
T4975 3454-3455 -LRB- denotes (
T4976 3462-3464 NN denotes 1C
T4977 3455-3461 NN denotes Figure
T4978 3464-3465 -RRB- denotes )
T4979 3465-3466 . denotes .
T4980 3466-3591 sentence denotes These results suggested that deletion of exon 14 indeed results in either loss or abnormal function of the Apc gene product.
T4981 3467-3472 DT denotes These
T4982 3473-3480 NNS denotes results
T4983 3481-3490 VBD denotes suggested
T4984 3491-3495 IN denotes that
T4985 3523-3530 VBZ denotes results
T4986 3496-3504 NN denotes deletion
T4987 3505-3507 IN denotes of
T4988 3508-3512 NN denotes exon
T4989 3513-3515 CD denotes 14
T4990 3516-3522 RB denotes indeed
T4991 3531-3533 IN denotes in
T4992 3534-3540 CC denotes either
T4993 3541-3545 NN denotes loss
T4994 3546-3548 CC denotes or
T4995 3549-3557 JJ denotes abnormal
T4996 3558-3566 NN denotes function
T4997 3567-3569 IN denotes of
T4998 3570-3573 DT denotes the
T4999 3583-3590 NN denotes product
T5000 3574-3577 NN denotes Apc
T5001 3578-3582 NN denotes gene
T5002 3590-3591 . denotes .
T5003 3591-3709 sentence denotes ApcΔ580/+ mice have median survival of 5 mo of age (Figure 1C), with progressive signs of rectal bleeding and anemia.
T5004 3592-3599 NN denotes ApcΔ580
T5005 3602-3606 NNS denotes mice
T5006 3599-3600 HYPH denotes /
T5007 3600-3601 SYM denotes +
T5008 3607-3611 VBP denotes have
T5009 3612-3618 JJ denotes median
T5010 3619-3627 NN denotes survival
T5011 3628-3630 IN denotes of
T5012 3631-3632 CD denotes 5
T5013 3633-3635 NNS denotes mo
T5014 3636-3638 IN denotes of
T5015 3639-3642 NN denotes age
T5016 3643-3644 -LRB- denotes (
T5017 3651-3653 NN denotes 1C
T5018 3644-3650 NN denotes Figure
T5019 3653-3654 -RRB- denotes )
T5020 3654-3656 , denotes ,
T5021 3656-3660 IN denotes with
T5022 3661-3672 JJ denotes progressive
T5023 3673-3678 NNS denotes signs
T5024 3679-3681 IN denotes of
T5025 3682-3688 JJ denotes rectal
T5026 3689-3697 NN denotes bleeding
T5027 3698-3701 CC denotes and
T5028 3702-3708 NN denotes anemia
T5029 3708-3709 . denotes .
T5030 3709-3924 sentence denotes Similar to the results reported with an independently generated ApcΔ580/+ conditional mouse strain [14], ApcΔ580/+ mice had more than 100 (120 ± 37, n = 11) intestinal tumors at the time of their death (Figure S1).
T5031 3710-3717 JJ denotes Similar
T5032 3830-3833 VBD denotes had
T5033 3718-3720 IN denotes to
T5034 3721-3724 DT denotes the
T5035 3725-3732 NNS denotes results
T5036 3733-3741 VBN denotes reported
T5037 3742-3746 IN denotes with
T5038 3747-3749 DT denotes an
T5039 3802-3808 NN denotes strain
T5040 3750-3763 RB denotes independently
T5041 3764-3773 VBN denotes generated
T5042 3774-3781 NN denotes ApcΔ580
T5043 3784-3795 JJ denotes conditional
T5044 3781-3782 HYPH denotes /
T5045 3782-3783 SYM denotes +
T5046 3796-3801 NN denotes mouse
T5047 3809-3810 -LRB- denotes [
T5048 3810-3812 CD denotes 14
T5049 3812-3813 -RRB- denotes ]
T5050 3813-3815 , denotes ,
T5051 3815-3822 NN denotes ApcΔ580
T5052 3825-3829 NNS denotes mice
T5053 3822-3823 HYPH denotes /
T5054 3823-3824 SYM denotes +
T5055 3834-3838 JJR denotes more
T5056 3844-3847 CD denotes 100
T5057 3839-3843 IN denotes than
T5058 3878-3884 NNS denotes tumors
T5059 3848-3849 -LRB- denotes (
T5060 3855-3857 CD denotes 37
T5061 3849-3852 CD denotes 120
T5062 3853-3854 SYM denotes ±
T5063 3857-3859 , denotes ,
T5064 3859-3860 NN denotes n
T5065 3863-3865 CD denotes 11
T5066 3861-3862 SYM denotes =
T5067 3865-3866 -RRB- denotes )
T5068 3867-3877 JJ denotes intestinal
T5069 3885-3887 IN denotes at
T5070 3888-3891 DT denotes the
T5071 3892-3896 NN denotes time
T5072 3897-3899 IN denotes of
T5073 3900-3905 PRP$ denotes their
T5074 3906-3911 NN denotes death
T5075 3912-3913 -LRB- denotes (
T5076 3920-3922 NN denotes S1
T5077 3913-3919 NN denotes Figure
T5078 3922-3923 -RRB- denotes )
T5079 3923-3924 . denotes .
T5080 3924-4006 sentence denotes Inactivation of wild-type Apc is an important prerequisite for tumor development.
T5081 3925-3937 NN denotes Inactivation
T5082 3955-3957 VBZ denotes is
T5083 3938-3940 IN denotes of
T5084 3941-3945 JJ denotes wild
T5085 3946-3950 NN denotes type
T5086 3945-3946 HYPH denotes -
T5087 3951-3954 NN denotes Apc
T5088 3958-3960 DT denotes an
T5089 3971-3983 NN denotes prerequisite
T5090 3961-3970 JJ denotes important
T5091 3984-3987 IN denotes for
T5092 3988-3993 NN denotes tumor
T5093 3994-4005 NN denotes development
T5094 4005-4006 . denotes .
T5095 4006-4267 sentence denotes We analyzed 30 intestinal tumors from ApcΔ580/+ mice by in vitro transcription and translation assay, but none of them showed truncated Apc products (unpublished data), indicating that the most likely mechanism of wild-type Apc inactivation is by allelic loss.
T5096 4007-4009 PRP denotes We
T5097 4010-4018 VBD denotes analyzed
T5098 4019-4021 CD denotes 30
T5099 4033-4039 NNS denotes tumors
T5100 4022-4032 JJ denotes intestinal
T5101 4040-4044 IN denotes from
T5102 4045-4052 JJ denotes ApcΔ580
T5103 4055-4059 NNS denotes mice
T5104 4052-4053 HYPH denotes /
T5105 4053-4054 SYM denotes +
T5106 4060-4062 IN denotes by
T5107 4063-4065 FW denotes in
T5108 4066-4071 FW denotes vitro
T5109 4072-4085 NN denotes transcription
T5110 4086-4089 CC denotes and
T5111 4090-4101 NN denotes translation
T5112 4102-4107 NN denotes assay
T5113 4107-4109 , denotes ,
T5114 4109-4112 CC denotes but
T5115 4113-4117 NN denotes none
T5116 4126-4132 VBD denotes showed
T5117 4118-4120 IN denotes of
T5118 4121-4125 PRP denotes them
T5119 4133-4142 VBN denotes truncated
T5120 4147-4155 NNS denotes products
T5121 4143-4146 NN denotes Apc
T5122 4156-4157 -LRB- denotes (
T5123 4169-4173 NNS denotes data
T5124 4157-4168 JJ denotes unpublished
T5125 4173-4174 -RRB- denotes )
T5126 4174-4176 , denotes ,
T5127 4176-4186 VBG denotes indicating
T5128 4187-4191 IN denotes that
T5129 4248-4250 VBZ denotes is
T5130 4192-4195 DT denotes the
T5131 4208-4217 NN denotes mechanism
T5132 4196-4200 RBS denotes most
T5133 4201-4207 JJ denotes likely
T5134 4218-4220 IN denotes of
T5135 4221-4225 JJ denotes wild
T5136 4226-4230 NN denotes type
T5137 4225-4226 HYPH denotes -
T5138 4235-4247 NN denotes inactivation
T5139 4231-4234 NN denotes Apc
T5140 4251-4253 IN denotes by
T5141 4254-4261 JJ denotes allelic
T5142 4262-4266 NN denotes loss
T5143 4266-4267 . denotes .
T5144 4267-4465 sentence denotes The mutant allele had to be maintained and transmitted through male mice, as ApcΔ580/+ females were frequently not healthy enough to successfully nurse their own pups because of their tumor burden.
T5145 4268-4271 DT denotes The
T5146 4279-4285 NN denotes allele
T5147 4272-4278 JJ denotes mutant
T5148 4286-4289 VBD denotes had
T5149 4290-4292 TO denotes to
T5150 4296-4306 VBN denotes maintained
T5151 4293-4295 VB denotes be
T5152 4307-4310 CC denotes and
T5153 4311-4322 VBN denotes transmitted
T5154 4323-4330 IN denotes through
T5155 4331-4335 JJ denotes male
T5156 4336-4340 NNS denotes mice
T5157 4340-4342 , denotes ,
T5158 4342-4344 IN denotes as
T5159 4363-4367 VBD denotes were
T5160 4345-4352 NN denotes ApcΔ580
T5161 4355-4362 NNS denotes females
T5162 4352-4353 HYPH denotes /
T5163 4353-4354 SYM denotes +
T5164 4368-4378 RB denotes frequently
T5165 4379-4382 RB denotes not
T5166 4383-4390 JJ denotes healthy
T5167 4391-4397 RB denotes enough
T5168 4398-4400 TO denotes to
T5169 4414-4419 VB denotes nurse
T5170 4401-4413 RB denotes successfully
T5171 4420-4425 PRP$ denotes their
T5172 4430-4434 NNS denotes pups
T5173 4426-4429 JJ denotes own
T5174 4435-4442 IN denotes because
T5175 4443-4445 IN denotes of
T5176 4446-4451 PRP$ denotes their
T5177 4458-4464 NN denotes burden
T5178 4452-4457 NN denotes tumor
T5179 4464-4465 . denotes .
T5180 4465-4531 sentence denotes ApcCKO/+ mice were intercrossed to generate ApcCKO/CKO offspring.
T5181 4466-4472 NN denotes ApcCKO
T5182 4475-4479 NNS denotes mice
T5183 4472-4473 HYPH denotes /
T5184 4473-4474 SYM denotes +
T5185 4485-4497 VBN denotes intercrossed
T5186 4480-4484 VBD denotes were
T5187 4498-4500 TO denotes to
T5188 4501-4509 VB denotes generate
T5189 4510-4516 NN denotes ApcCKO
T5190 4517-4520 NN denotes CKO
T5191 4516-4517 HYPH denotes /
T5192 4521-4530 NN denotes offspring
T5193 4530-4531 . denotes .
T5194 4531-4624 sentence denotes Approximately one-quarter of the offspring (17 of 81) were homozygous for the ApcCKO allele.
T5195 4532-4545 RB denotes Approximately
T5196 4546-4549 CD denotes one
T5197 4586-4590 VBD denotes were
T5198 4549-4550 HYPH denotes -
T5199 4550-4557 JJ denotes quarter
T5200 4558-4560 IN denotes of
T5201 4561-4564 DT denotes the
T5202 4565-4574 NN denotes offspring
T5203 4575-4576 -LRB- denotes (
T5204 4576-4578 CD denotes 17
T5205 4579-4581 IN denotes of
T5206 4582-4584 CD denotes 81
T5207 4584-4585 -RRB- denotes )
T5208 4591-4601 JJ denotes homozygous
T5209 4602-4605 IN denotes for
T5210 4606-4609 DT denotes the
T5211 4617-4623 NN denotes allele
T5212 4610-4616 NN denotes ApcCKO
T5213 4623-4624 . denotes .
T5214 4624-4777 sentence denotes These mice as well as heterozygous mice for ApcCKO allele are normal, showing no differences in their survival to the wild-type littermates (Figure 1C).
T5215 4625-4630 DT denotes These
T5216 4631-4635 NNS denotes mice
T5217 4683-4686 VBP denotes are
T5218 4636-4638 RB denotes as
T5219 4644-4646 IN denotes as
T5220 4639-4643 RB denotes well
T5221 4647-4659 JJ denotes heterozygous
T5222 4660-4664 NNS denotes mice
T5223 4665-4668 IN denotes for
T5224 4669-4675 NN denotes ApcCKO
T5225 4676-4682 NN denotes allele
T5226 4687-4693 JJ denotes normal
T5227 4693-4695 , denotes ,
T5228 4695-4702 VBG denotes showing
T5229 4703-4705 DT denotes no
T5230 4706-4717 NNS denotes differences
T5231 4718-4720 IN denotes in
T5232 4721-4726 PRP$ denotes their
T5233 4727-4735 NN denotes survival
T5234 4736-4738 IN denotes to
T5235 4739-4742 DT denotes the
T5236 4753-4764 NNS denotes littermates
T5237 4743-4747 JJ denotes wild
T5238 4748-4752 NN denotes type
T5239 4747-4748 HYPH denotes -
T5240 4765-4766 -LRB- denotes (
T5241 4773-4775 NN denotes 1C
T5242 4766-4772 NN denotes Figure
T5243 4775-4776 -RRB- denotes )
T5244 4776-4777 . denotes .
T5245 4777-4910 sentence denotes We tested whether our ApcCKO allele can compliment the wild-type allele by crossing the ApcCKO/CKO female with ApcΔ580/+ male mouse.
T5246 4778-4780 PRP denotes We
T5247 4781-4787 VBD denotes tested
T5248 4788-4795 IN denotes whether
T5249 4818-4828 VB denotes compliment
T5250 4796-4799 PRP$ denotes our
T5251 4807-4813 NN denotes allele
T5252 4800-4806 NN denotes ApcCKO
T5253 4814-4817 MD denotes can
T5254 4829-4832 DT denotes the
T5255 4843-4849 NN denotes allele
T5256 4833-4837 JJ denotes wild
T5257 4838-4842 NN denotes type
T5258 4837-4838 HYPH denotes -
T5259 4850-4852 IN denotes by
T5260 4853-4861 VBG denotes crossing
T5261 4862-4865 DT denotes the
T5262 4877-4883 NN denotes female
T5263 4866-4872 NN denotes ApcCKO
T5264 4873-4876 NN denotes CKO
T5265 4872-4873 HYPH denotes /
T5266 4884-4888 IN denotes with
T5267 4889-4896 NN denotes ApcΔ580
T5268 4904-4909 NN denotes mouse
T5269 4896-4897 HYPH denotes /
T5270 4897-4898 SYM denotes +
T5271 4899-4903 JJ denotes male
T5272 4909-4910 . denotes .
T5273 4910-5118 sentence denotes The resultant ApcCKO/Δ580 offspring were viable and born in the Mendelian ratio, suggesting that the presence of loxP sites in introns flanking exon 14 have no adverse effect on the function of the Apc gene.
T5274 4911-4914 DT denotes The
T5275 4937-4946 NN denotes offspring
T5276 4915-4924 JJ denotes resultant
T5277 4925-4931 NN denotes ApcCKO
T5278 4932-4936 NN denotes Δ580
T5279 4931-4932 HYPH denotes /
T5280 4947-4951 VBD denotes were
T5281 4952-4958 JJ denotes viable
T5282 4959-4962 CC denotes and
T5283 4963-4967 VBN denotes born
T5284 4968-4970 IN denotes in
T5285 4971-4974 DT denotes the
T5286 4985-4990 NN denotes ratio
T5287 4975-4984 JJ denotes Mendelian
T5288 4990-4992 , denotes ,
T5289 4992-5002 VBG denotes suggesting
T5290 5003-5007 IN denotes that
T5291 5063-5067 VBP denotes have
T5292 5008-5011 DT denotes the
T5293 5012-5020 NN denotes presence
T5294 5021-5023 IN denotes of
T5295 5024-5028 NN denotes loxP
T5296 5029-5034 NNS denotes sites
T5297 5035-5037 IN denotes in
T5298 5038-5045 NNS denotes introns
T5299 5046-5054 VBG denotes flanking
T5300 5055-5059 NN denotes exon
T5301 5060-5062 CD denotes 14
T5302 5068-5070 DT denotes no
T5303 5079-5085 NN denotes effect
T5304 5071-5078 JJ denotes adverse
T5305 5086-5088 IN denotes on
T5306 5089-5092 DT denotes the
T5307 5093-5101 NN denotes function
T5308 5102-5104 IN denotes of
T5309 5105-5108 DT denotes the
T5310 5113-5117 NN denotes gene
T5311 5109-5112 NN denotes Apc
T5312 5117-5118 . denotes .
R918 T4598 T4597 prep of,Generation
R919 T4599 T4600 det the,Mice
R920 T4600 T4598 pobj Mice,of
R921 T4601 T4600 nmod ApcCKO,Mice
R922 T4602 T4601 cc and,ApcCKO
R923 T4603 T4601 conj ApcΔ580,ApcCKO
R924 T4605 T4606 aux To,investigate
R925 T4606 T4607 advcl investigate,used
R926 T4608 T4609 det the,role
R927 T4609 T4606 dobj role,investigate
R928 T4610 T4609 prep of,role
R929 T4611 T4610 pobj Apc,of
R930 T4612 T4609 prep in,role
R931 T4613 T4612 pobj development,in
R932 T4614 T4613 prep of,development
R933 T4615 T4614 pobj skin,of
R934 T4616 T4615 cc and,skin
R935 T4617 T4618 poss its,appendages
R936 T4618 T4615 conj appendages,skin
R937 T4619 T4607 punct ", ",used
R938 T4620 T4607 nsubj we,used
R939 T4621 T4622 det the,technology
R940 T4622 T4607 dobj technology,used
R941 T4623 T4624 compound Cre,loxP
R942 T4624 T4622 compound loxP,technology
R943 T4625 T4624 punct /,loxP
R944 T4626 T4627 aux to,introduce
R945 T4627 T4607 advcl introduce,used
R946 T4628 T4629 det a,mutation
R947 T4629 T4627 dobj mutation,introduce
R948 T4630 T4629 amod conditional,mutation
R949 T4631 T4629 prep of,mutation
R950 T4632 T4633 det the,gene
R951 T4633 T4631 pobj gene,of
R952 T4634 T4633 compound Apc,gene
R953 T4635 T4627 prep in,introduce
R954 T4636 T4635 pobj mice,in
R955 T4637 T4607 punct .,used
R956 T4639 T4640 nsubj We,constructed
R957 T4641 T4642 amod embryonic,stem
R958 T4642 T4643 nmod stem,cells
R959 T4643 T4640 dobj cells,constructed
R960 T4644 T4642 punct (,stem
R961 T4645 T4642 appos ES,stem
R962 T4646 T4643 punct ),cells
R963 T4647 T4643 cc and,cells
R964 T4648 T4643 conj mice,cells
R965 T4649 T4648 acl carrying,mice
R966 T4650 T4651 det an,allele
R967 T4651 T4649 dobj allele,carrying
R968 T4652 T4651 compound Apc,allele
R969 T4653 T4651 acl harboring,allele
R970 T4654 T4655 preconj both,pair
R971 T4655 T4653 dobj pair,harboring
R972 T4656 T4655 det a,pair
R973 T4657 T4655 prep of,pair
R974 T4658 T4659 compound loxP,sites
R975 T4659 T4657 pobj sites,of
R976 T4660 T4655 acl flanking,pair
R977 T4661 T4662 compound Apc,exon
R978 T4662 T4660 dobj exon,flanking
R979 T4663 T4662 nummod 14,exon
R980 T4664 T4655 cc and,pair
R981 T4665 T4666 det a,pair
R982 T4666 T4655 conj pair,pair
R983 T4667 T4666 prep of,pair
R984 T4668 T4669 nmod FLP,target
R985 T4669 T4671 nmod target,sites
R986 T4670 T4669 nmod recognition,target
R987 T4671 T4667 pobj sites,of
R988 T4672 T4669 punct (,target
R989 T4673 T4669 appos FRT,target
R990 T4674 T4671 punct ),sites
R991 T4675 T4666 acl flanking,pair
R992 T4676 T4677 compound PGK,neomycin
R993 T4677 T4679 compound neomycin,cassette
R994 T4678 T4677 punct -,neomycin
R995 T4679 T4675 dobj cassette,flanking
R996 T4680 T4679 compound selection,cassette
R997 T4681 T4640 prep by,constructed
R998 T4682 T4681 pobj recombineering,by
R999 T4683 T4684 punct [,13
R1000 T4684 T4640 parataxis 13,constructed
R1001 T4685 T4684 nummod 12,13
R1002 T4686 T4684 punct ",",13
R1003 T4687 T4684 punct ],13
R1004 T4688 T4689 punct (,N
R1005 T4689 T4640 parataxis N,constructed
R1006 T4690 T4691 compound Figure,1A
R1007 T4691 T4689 dep 1A,N
R1008 T4692 T4689 punct ", ",N
R1009 T4693 T4694 compound ApcCKON,allele
R1010 T4694 T4689 dep allele,N
R1011 T4695 T4689 punct ", ",N
R1012 T4696 T4689 prep for,N
R1013 T4697 T4698 compound neomycin,cassette
R1014 T4698 T4696 pobj cassette,for
R1015 T4699 T4689 punct ),N
R1016 T4700 T4640 punct .,constructed
R1017 T4702 T4703 det A,cassette
R1018 T4703 T4707 nsubjpass cassette,inserted
R1019 T4704 T4705 compound PGK,neomycin
R1020 T4705 T4703 compound neomycin,cassette
R1021 T4706 T4705 punct -,neomycin
R1022 T4708 T4707 auxpass was,inserted
R1023 T4709 T4707 prep in,inserted
R1024 T4710 T4711 det the,orientation
R1025 T4711 T4709 pobj orientation,in
R1026 T4712 T4711 amod same,orientation
R1027 T4713 T4711 amod transcriptional,orientation
R1028 T4714 T4711 prep as,orientation
R1029 T4715 T4714 pobj Apc,as
R1030 T4716 T4707 prep in,inserted
R1031 T4717 T4716 pobj intron,in
R1032 T4718 T4717 nummod 14,intron
R1033 T4719 T4717 prep of,intron
R1034 T4720 T4721 det the,gene
R1035 T4721 T4719 pobj gene,of
R1036 T4722 T4721 amod endogenous,gene
R1037 T4723 T4707 punct .,inserted
R1038 T4725 T4726 det The,sites
R1039 T4726 T4730 nsubjpass sites,used
R1040 T4727 T4726 nmod loxP,sites
R1041 T4728 T4727 cc and,loxP
R1042 T4729 T4727 conj FRT,loxP
R1043 T4731 T4730 auxpass were,used
R1044 T4732 T4733 aux to,aid
R1045 T4733 T4730 advcl aid,used
R1046 T4734 T4735 amod unidirectional,recombination
R1047 T4735 T4733 dobj recombination,aid
R1048 T4736 T4737 punct [,13
R1049 T4737 T4730 parataxis 13,used
R1050 T4738 T4737 nummod 12,13
R1051 T4739 T4737 punct ",",13
R1052 T4740 T4737 punct ],13
R1053 T4741 T4730 punct .,used
R1054 T4743 T4744 nummod Two,lines
R1055 T4744 T4746 nsubjpass lines,generated
R1056 T4745 T4744 compound mouse,lines
R1057 T4747 T4744 acl containing,lines
R1058 T4748 T4749 det the,modification
R1059 T4749 T4747 dobj modification,containing
R1060 T4750 T4749 amod same,modification
R1061 T4751 T4746 auxpass were,generated
R1062 T4752 T4746 prep from,generated
R1063 T4753 T4754 nummod two,clones
R1064 T4754 T4752 pobj clones,from
R1065 T4755 T4754 amod independent,clones
R1066 T4756 T4754 compound ES,clones
R1067 T4757 T4758 aux to,ensure
R1068 T4758 T4746 advcl ensure,generated
R1069 T4759 T4760 mark that,behave
R1070 T4760 T4758 ccomp behave,ensure
R1071 T4761 T4762 det these,lines
R1072 T4762 T4760 nsubj lines,behave
R1073 T4763 T4762 nummod two,lines
R1074 T4764 T4760 prep in,behave
R1075 T4765 T4766 det the,way
R1076 T4766 T4764 pobj way,in
R1077 T4767 T4766 amod same,way
R1078 T4768 T4746 punct .,generated
R1079 T4770 T4771 det These,mice
R1080 T4771 T4775 nsubjpass mice,crossed
R1081 T4772 T4771 nmod ApcCKON,mice
R1082 T4773 T4772 punct /,ApcCKON
R1083 T4774 T4772 punct +,ApcCKON
R1084 T4776 T4775 auxpass were,crossed
R1085 T4777 T4775 prep with,crossed
R1086 T4778 T4779 compound FLPe,deleter
R1087 T4779 T4777 pobj deleter,with
R1088 T4780 T4779 punct -,deleter
R1089 T4781 T4782 aux to,generate
R1090 T4782 T4775 advcl generate,crossed
R1091 T4783 T4784 nmod ApcCKO,mice
R1092 T4784 T4782 dobj mice,generate
R1093 T4785 T4783 punct /,ApcCKO
R1094 T4786 T4783 punct +,ApcCKO
R1095 T4787 T4788 dep that,were
R1096 T4788 T4784 relcl were,mice
R1097 T4789 T4788 acomp heterozygous,were
R1098 T4790 T4789 prep for,heterozygous
R1099 T4791 T4792 det the,allele
R1100 T4792 T4790 pobj allele,for
R1101 T4793 T4792 amod final,allele
R1102 T4794 T4795 npadvmod Apc,conditional
R1103 T4795 T4792 amod conditional,allele
R1104 T4796 T4797 punct (,ApcCKO
R1105 T4797 T4795 parataxis ApcCKO,conditional
R1106 T4798 T4797 punct ),ApcCKO
R1107 T4799 T4800 dep that,removed
R1108 T4800 T4792 relcl removed,allele
R1109 T4801 T4802 det the,cassette
R1110 T4802 T4800 dobj cassette,removed
R1111 T4803 T4804 compound PGK,neomycin
R1112 T4804 T4802 compound neomycin,cassette
R1113 T4805 T4804 punct -,neomycin
R1114 T4806 T4800 cc and,removed
R1115 T4807 T4800 conj contains,removed
R1116 T4808 T4809 advmod only,sites
R1117 T4809 T4807 dobj sites,contains
R1118 T4810 T4809 det the,sites
R1119 T4811 T4809 compound loxP,sites
R1120 T4812 T4807 prep in,contains
R1121 T4813 T4814 det the,introns
R1122 T4814 T4812 pobj introns,in
R1123 T4815 T4814 acl flanking,introns
R1124 T4816 T4815 dobj exon,flanking
R1125 T4817 T4816 nummod 14,exon
R1126 T4818 T4775 punct .,crossed
R1127 T4820 T4821 aux To,assess
R1128 T4821 T4822 advcl assess,crossed
R1129 T4823 T4824 det the,effect
R1130 T4824 T4821 dobj effect,assess
R1131 T4825 T4824 prep of,effect
R1132 T4826 T4825 pcomp deleting,of
R1133 T4827 T4826 dobj exon,deleting
R1134 T4828 T4827 nummod 14,exon
R1135 T4829 T4821 prep in,assess
R1136 T4830 T4829 pobj mice,in
R1137 T4831 T4822 punct ", ",crossed
R1138 T4832 T4833 det both,lines
R1139 T4833 T4822 nsubjpass lines,crossed
R1140 T4834 T4833 prep of,lines
R1141 T4835 T4836 nmod ApcCKO,mice
R1142 T4836 T4834 pobj mice,of
R1143 T4837 T4835 punct /,ApcCKO
R1144 T4838 T4835 punct +,ApcCKO
R1145 T4839 T4822 auxpass were,crossed
R1146 T4840 T4822 prep with,crossed
R1147 T4841 T4842 det the,deleter
R1148 T4842 T4840 pobj deleter,with
R1149 T4843 T4842 compound Cre,deleter
R1150 T4844 T4842 punct -,deleter
R1151 T4845 T4846 aux to,generate
R1152 T4846 T4822 advcl generate,crossed
R1153 T4847 T4848 det the,line
R1154 T4848 T4846 dobj line,generate
R1155 T4849 T4850 compound germline,knockout
R1156 T4850 T4848 compound knockout,line
R1157 T4851 T4848 prep of,line
R1158 T4852 T4851 pobj Apc,of
R1159 T4853 T4848 punct ", ",line
R1160 T4854 T4848 acl designated,line
R1161 T4855 T4854 oprd ApcΔ580,designated
R1162 T4856 T4855 punct /,ApcΔ580
R1163 T4857 T4855 punct +,ApcΔ580
R1164 T4858 T4822 punct .,crossed
R1165 T4860 T4861 det The,allele
R1166 T4861 T4863 nsubj allele,lacks
R1167 T4862 T4861 amod mutant,allele
R1168 T4864 T4861 punct (,allele
R1169 T4865 T4861 appos ApcΔ580,allele
R1170 T4866 T4863 punct ),lacks
R1171 T4867 T4863 dobj exon,lacks
R1172 T4868 T4867 nummod 14,exon
R1173 T4869 T4870 punct (,1A
R1174 T4870 T4863 parataxis 1A,lacks
R1175 T4871 T4870 compound Figure,1A
R1176 T4872 T4870 punct ),1A
R1177 T4873 T4863 punct .,lacks
R1178 T4875 T4876 det The,transcript
R1179 T4876 T4877 nsubj transcript,results
R1180 T4878 T4876 prep from,transcript
R1181 T4879 T4878 pobj loss,from
R1182 T4880 T4879 prep of,loss
R1183 T4881 T4880 pobj exon,of
R1184 T4882 T4881 nummod 14,exon
R1185 T4883 T4877 prep in,results
R1186 T4884 T4885 det a,shift
R1187 T4885 T4883 pobj shift,in
R1188 T4886 T4885 prep in,shift
R1189 T4887 T4888 det the,frame
R1190 T4888 T4886 pobj frame,in
R1191 T4889 T4888 amod normal,frame
R1192 T4890 T4888 compound reading,frame
R1193 T4891 T4877 punct ", ",results
R1194 T4892 T4877 advcl resulting,results
R1195 T4893 T4892 prep in,resulting
R1196 T4894 T4895 det a,codon
R1197 T4895 T4893 pobj codon,in
R1198 T4896 T4895 amod premature,codon
R1199 T4897 T4898 compound chain,termination
R1200 T4898 T4895 compound termination,codon
R1201 T4899 T4900 dep which,result
R1202 T4900 T4895 relcl result,codon
R1203 T4901 T4900 punct ", ",result
R1204 T4902 T4903 mark if,utilized
R1205 T4903 T4900 advcl utilized,result
R1206 T4904 T4900 punct ", ",result
R1207 T4905 T4900 aux would,result
R1208 T4906 T4900 prep in,result
R1209 T4907 T4908 det a,polypeptide
R1210 T4908 T4906 pobj polypeptide,in
R1211 T4909 T4908 amod truncated,polypeptide
R1212 T4910 T4911 dep that,is
R1213 T4911 T4908 relcl is,polypeptide
R1214 T4912 T4913 nummod 605,aa
R1215 T4913 T4911 attr aa,is
R1216 T4914 T4913 prep in,aa
R1217 T4915 T4914 pobj length,in
R1218 T4916 T4913 punct ", ",aa
R1219 T4917 T4918 prep of,correspond
R1220 T4918 T4913 relcl correspond,aa
R1221 T4919 T4917 pobj which,of
R1222 T4920 T4921 det the,aa
R1223 T4921 T4918 nsubj aa,correspond
R1224 T4922 T4921 amod first,aa
R1225 T4923 T4921 nummod 580,aa
R1226 T4924 T4918 prep to,correspond
R1227 T4925 T4926 det the,protein
R1228 T4926 T4924 pobj protein,to
R1229 T4927 T4926 amod normal,protein
R1230 T4928 T4926 compound Apc,protein
R1231 T4929 T4877 punct .,results
R1232 T4931 T4932 compound Southern,blot
R1233 T4932 T4933 compound blot,analysis
R1234 T4933 T4934 nsubj analysis,confirmed
R1235 T4935 T4933 prep of,analysis
R1236 T4936 T4937 compound tail,DNA
R1237 T4937 T4935 pobj DNA,of
R1238 T4938 T4937 prep from,DNA
R1239 T4939 T4940 compound F1,offspring
R1240 T4940 T4938 pobj offspring,from
R1241 T4941 T4940 prep of,offspring
R1242 T4942 T4943 preconj both,ApcCKON
R1243 T4943 T4944 nmod ApcCKON,lines
R1244 T4944 T4941 pobj lines,of
R1245 T4945 T4943 cc and,ApcCKON
R1246 T4946 T4943 conj ApcΔ580,ApcCKON
R1247 T4947 T4948 det the,transmission
R1248 T4948 T4934 dobj transmission,confirmed
R1249 T4949 T4948 compound germline,transmission
R1250 T4950 T4948 prep of,transmission
R1251 T4951 T4952 amod modified,allele
R1252 T4952 T4950 pobj allele,of
R1253 T4953 T4952 compound Apc,allele
R1254 T4954 T4955 punct (,1B
R1255 T4955 T4934 parataxis 1B,confirmed
R1256 T4956 T4955 compound Figure,1B
R1257 T4957 T4955 punct ),1B
R1258 T4958 T4934 punct .,confirmed
R1259 T4960 T4961 nsubj Mice,are
R1260 T4962 T4963 dep that,are
R1261 T4963 T4960 relcl are,Mice
R1262 T4964 T4963 acomp heterozygous,are
R1263 T4965 T4964 prep for,heterozygous
R1264 T4966 T4967 compound ApcΔ580,mutation
R1265 T4967 T4965 pobj mutation,for
R1266 T4968 T4961 acomp viable,are
R1267 T4969 T4961 cc but,are
R1268 T4970 T4961 conj have,are
R1269 T4971 T4972 det a,lifespan
R1270 T4972 T4970 dobj lifespan,have
R1271 T4973 T4974 advmod significantly,reduced
R1272 T4974 T4972 amod reduced,lifespan
R1273 T4975 T4976 punct (,1C
R1274 T4976 T4970 parataxis 1C,have
R1275 T4977 T4976 compound Figure,1C
R1276 T4978 T4976 punct ),1C
R1277 T4979 T4961 punct .,are
R1278 T4981 T4982 det These,results
R1279 T4982 T4983 nsubj results,suggested
R1280 T4984 T4985 mark that,results
R1281 T4985 T4983 ccomp results,suggested
R1282 T4986 T4985 nsubj deletion,results
R1283 T4987 T4986 prep of,deletion
R1284 T4988 T4987 pobj exon,of
R1285 T4989 T4988 nummod 14,exon
R1286 T4990 T4985 advmod indeed,results
R1287 T4991 T4985 prep in,results
R1288 T4992 T4993 preconj either,loss
R1289 T4993 T4991 pobj loss,in
R1290 T4994 T4993 cc or,loss
R1291 T4995 T4996 amod abnormal,function
R1292 T4996 T4993 conj function,loss
R1293 T4997 T4993 prep of,loss
R1294 T4998 T4999 det the,product
R1295 T4999 T4997 pobj product,of
R1296 T5000 T5001 compound Apc,gene
R1297 T5001 T4999 compound gene,product
R1298 T5002 T4983 punct .,suggested
R1299 T5004 T5005 nmod ApcΔ580,mice
R1300 T5005 T5008 nsubj mice,have
R1301 T5006 T5004 punct /,ApcΔ580
R1302 T5007 T5004 punct +,ApcΔ580
R1303 T5009 T5010 amod median,survival
R1304 T5010 T5008 dobj survival,have
R1305 T5011 T5010 prep of,survival
R1306 T5012 T5013 nummod 5,mo
R1307 T5013 T5011 pobj mo,of
R1308 T5014 T5013 prep of,mo
R1309 T5015 T5014 pobj age,of
R1310 T5016 T5017 punct (,1C
R1311 T5017 T5010 parataxis 1C,survival
R1312 T5018 T5017 compound Figure,1C
R1313 T5019 T5017 punct ),1C
R1314 T5020 T5010 punct ", ",survival
R1315 T5021 T5010 prep with,survival
R1316 T5022 T5023 amod progressive,signs
R1317 T5023 T5021 pobj signs,with
R1318 T5024 T5023 prep of,signs
R1319 T5025 T5026 amod rectal,bleeding
R1320 T5026 T5024 pobj bleeding,of
R1321 T5131 T5129 nsubj mechanism,is
R1322 T5027 T5026 cc and,bleeding
R1323 T5028 T5026 conj anemia,bleeding
R1324 T5132 T5133 advmod most,likely
R1325 T5029 T5008 punct .,have
R1326 T5133 T5131 amod likely,mechanism
R1327 T5031 T5032 advcl Similar,had
R1328 T5033 T5031 prep to,Similar
R1329 T5134 T5131 prep of,mechanism
R1330 T5034 T5035 det the,results
R1331 T5035 T5033 pobj results,to
R1332 T5135 T5136 amod wild,type
R1333 T5036 T5035 acl reported,results
R1334 T5037 T5036 prep with,reported
R1335 T5038 T5039 det an,strain
R1336 T5136 T5138 compound type,inactivation
R1337 T5039 T5037 pobj strain,with
R1338 T5040 T5039 advmod independently,strain
R1339 T5041 T5039 amod generated,strain
R1340 T5137 T5136 punct -,type
R1341 T5042 T5043 npadvmod ApcΔ580,conditional
R1342 T5043 T5039 amod conditional,strain
R1343 T5044 T5042 punct /,ApcΔ580
R1344 T5138 T5134 pobj inactivation,of
R1345 T5045 T5042 punct +,ApcΔ580
R1346 T5046 T5039 compound mouse,strain
R1347 T5047 T5048 punct [,14
R1348 T5139 T5138 compound Apc,inactivation
R1349 T5048 T5036 parataxis 14,reported
R1350 T5049 T5048 punct ],14
R1351 T5050 T5032 punct ", ",had
R1352 T5140 T5129 prep by,is
R1353 T5051 T5052 nmod ApcΔ580,mice
R1354 T5052 T5032 nsubj mice,had
R1355 T5053 T5051 punct /,ApcΔ580
R1356 T5141 T5142 amod allelic,loss
R1357 T5054 T5051 punct +,ApcΔ580
R1358 T5055 T5056 amod more,100
R1359 T5056 T5058 nummod 100,tumors
R1360 T5142 T5140 pobj loss,by
R1361 T5057 T5056 quantmod than,100
R1362 T5143 T5116 punct .,showed
R1363 T5058 T5032 dobj tumors,had
R1364 T5059 T5060 punct (,37
R1365 T5060 T5056 parataxis 37,100
R1366 T5061 T5060 nummod 120,37
R1367 T5062 T5060 punct ±,37
R1368 T5145 T5146 det The,allele
R1369 T5063 T5060 punct ", ",37
R1370 T5064 T5065 nsubj n,11
R1371 T5065 T5060 ccomp 11,37
R1372 T5066 T5065 punct =,11
R1373 T5067 T5060 punct ),37
R1374 T5068 T5058 amod intestinal,tumors
R1375 T5069 T5032 prep at,had
R1376 T5070 T5071 det the,time
R1377 T5146 T5148 nsubj allele,had
R1378 T5071 T5069 pobj time,at
R1379 T5072 T5071 prep of,time
R1380 T5073 T5074 poss their,death
R1381 T5074 T5072 pobj death,of
R1382 T5147 T5146 amod mutant,allele
R1383 T5075 T5076 punct (,S1
R1384 T5076 T5032 parataxis S1,had
R1385 T5077 T5076 compound Figure,S1
R1386 T5149 T5150 aux to,maintained
R1387 T5078 T5076 punct ),S1
R1388 T5079 T5032 punct .,had
R1389 T5081 T5082 nsubj Inactivation,is
R1390 T5150 T5148 xcomp maintained,had
R1391 T5083 T5081 prep of,Inactivation
R1392 T5084 T5085 amod wild,type
R1393 T5151 T5150 auxpass be,maintained
R1394 T5085 T5087 compound type,Apc
R1395 T5086 T5085 punct -,type
R1396 T5087 T5083 pobj Apc,of
R1397 T5152 T5150 cc and,maintained
R1398 T5088 T5089 det an,prerequisite
R1399 T5089 T5082 attr prerequisite,is
R1400 T5090 T5089 amod important,prerequisite
R1401 T5153 T5150 conj transmitted,maintained
R1402 T5091 T5089 prep for,prerequisite
R1403 T5092 T5093 compound tumor,development
R1404 T5093 T5091 pobj development,for
R1405 T5154 T5153 prep through,transmitted
R1406 T5094 T5082 punct .,is
R1407 T5155 T5156 amod male,mice
R1408 T5096 T5097 nsubj We,analyzed
R1409 T5098 T5099 nummod 30,tumors
R1410 T5156 T5154 pobj mice,through
R1411 T5099 T5097 dobj tumors,analyzed
R1412 T5100 T5099 amod intestinal,tumors
R1413 T5157 T5153 punct ", ",transmitted
R1414 T5101 T5099 prep from,tumors
R1415 T5102 T5103 nmod ApcΔ580,mice
R1416 T5103 T5101 pobj mice,from
R1417 T5104 T5102 punct /,ApcΔ580
R1418 T5158 T5159 mark as,were
R1419 T5105 T5102 punct +,ApcΔ580
R1420 T5106 T5097 prep by,analyzed
R1421 T5159 T5153 advcl were,transmitted
R1422 T5160 T5161 nmod ApcΔ580,females
R1423 T5107 T5108 advmod in,vitro
R1424 T5108 T5109 amod vitro,transcription
R1425 T5109 T5106 pobj transcription,by
R1426 T5110 T5109 cc and,transcription
R1427 T5111 T5112 compound translation,assay
R1428 T5112 T5109 conj assay,transcription
R1429 T5161 T5159 nsubj females,were
R1430 T5113 T5097 punct ", ",analyzed
R1431 T5114 T5097 cc but,analyzed
R1432 T5115 T5116 nsubj none,showed
R1433 T5162 T5160 punct /,ApcΔ580
R1434 T5116 T5097 conj showed,analyzed
R1435 T5117 T5115 prep of,none
R1436 T5163 T5160 punct +,ApcΔ580
R1437 T5118 T5117 pobj them,of
R1438 T5119 T5120 amod truncated,products
R1439 T5120 T5116 dobj products,showed
R1440 T5164 T5159 advmod frequently,were
R1441 T5121 T5120 compound Apc,products
R1442 T5122 T5123 punct (,data
R1443 T5123 T5116 meta data,showed
R1444 T5165 T5159 neg not,were
R1445 T5124 T5123 amod unpublished,data
R1446 T5125 T5116 punct ),showed
R1447 T5126 T5116 punct ", ",showed
R1448 T5166 T5159 acomp healthy,were
R1449 T5127 T5116 advcl indicating,showed
R1450 T5128 T5129 mark that,is
R1451 T5129 T5127 ccomp is,indicating
R1452 T5167 T5166 advmod enough,healthy
R1453 T5130 T5131 det the,mechanism
R1454 T5168 T5169 aux to,nurse
R1455 T5169 T5166 xcomp nurse,healthy
R1456 T5170 T5169 advmod successfully,nurse
R1457 T5171 T5172 poss their,pups
R1458 T5237 T5238 amod wild,type
R1459 T5172 T5169 dobj pups,nurse
R1460 T5238 T5236 compound type,littermates
R1461 T5239 T5238 punct -,type
R1462 T5173 T5172 amod own,pups
R1463 T5240 T5241 punct (,1C
R1464 T5241 T5228 parataxis 1C,showing
R1465 T5242 T5241 compound Figure,1C
R1466 T5174 T5159 prep because,were
R1467 T5243 T5241 punct ),1C
R1468 T5244 T5217 punct .,are
R1469 T5175 T5174 pcomp of,because
R1470 T5246 T5247 nsubj We,tested
R1471 T5176 T5177 poss their,burden
R1472 T5248 T5249 mark whether,compliment
R1473 T5249 T5247 ccomp compliment,tested
R1474 T5177 T5174 pobj burden,because
R1475 T5250 T5251 poss our,allele
R1476 T5251 T5249 nsubj allele,compliment
R1477 T5252 T5251 compound ApcCKO,allele
R1478 T5253 T5249 aux can,compliment
R1479 T5254 T5255 det the,allele
R1480 T5255 T5249 dobj allele,compliment
R1481 T5256 T5257 amod wild,type
R1482 T5178 T5177 compound tumor,burden
R1483 T5257 T5255 compound type,allele
R1484 T5258 T5257 punct -,type
R1485 T5259 T5249 prep by,compliment
R1486 T5179 T5148 punct .,had
R1487 T5260 T5259 pcomp crossing,by
R1488 T5261 T5262 det the,female
R1489 T5262 T5260 dobj female,crossing
R1490 T5181 T5182 nmod ApcCKO,mice
R1491 T5263 T5264 compound ApcCKO,CKO
R1492 T5264 T5262 compound CKO,female
R1493 T5265 T5264 punct /,CKO
R1494 T5266 T5260 prep with,crossing
R1495 T5267 T5268 nmod ApcΔ580,mouse
R1496 T5268 T5266 pobj mouse,with
R1497 T5182 T5185 nsubjpass mice,intercrossed
R1498 T5269 T5267 punct /,ApcΔ580
R1499 T5270 T5267 punct +,ApcΔ580
R1500 T5271 T5268 amod male,mouse
R1501 T5183 T5181 punct /,ApcCKO
R1502 T5272 T5247 punct .,tested
R1503 T5184 T5181 punct +,ApcCKO
R1504 T5274 T5275 det The,offspring
R1505 T5275 T5280 nsubj offspring,were
R1506 T5276 T5275 amod resultant,offspring
R1507 T5186 T5185 auxpass were,intercrossed
R1508 T5277 T5278 compound ApcCKO,Δ580
R1509 T5278 T5275 compound Δ580,offspring
R1510 T5279 T5278 punct /,Δ580
R1511 T5187 T5188 aux to,generate
R1512 T5188 T5185 advcl generate,intercrossed
R1513 T5189 T5190 compound ApcCKO,CKO
R1514 T5281 T5280 acomp viable,were
R1515 T5190 T5192 compound CKO,offspring
R1516 T5191 T5190 punct /,CKO
R1517 T5282 T5281 cc and,viable
R1518 T5283 T5281 conj born,viable
R1519 T5192 T5188 dobj offspring,generate
R1520 T5284 T5283 prep in,born
R1521 T5285 T5286 det the,ratio
R1522 T5286 T5284 pobj ratio,in
R1523 T5193 T5185 punct .,intercrossed
R1524 T5287 T5286 amod Mendelian,ratio
R1525 T5288 T5280 punct ", ",were
R1526 T5289 T5280 advcl suggesting,were
R1527 T5195 T5196 advmod Approximately,one
R1528 T5290 T5291 mark that,have
R1529 T5291 T5289 ccomp have,suggesting
R1530 T5292 T5293 det the,presence
R1531 T5293 T5291 nsubj presence,have
R1532 T5294 T5293 prep of,presence
R1533 T5196 T5197 nsubj one,were
R1534 T5295 T5296 compound loxP,sites
R1535 T5296 T5294 pobj sites,of
R1536 T5297 T5293 prep in,presence
R1537 T5198 T5196 punct -,one
R1538 T5298 T5297 pobj introns,in
R1539 T5299 T5298 acl flanking,introns
R1540 T5300 T5299 dobj exon,flanking
R1541 T5301 T5300 nummod 14,exon
R1542 T5199 T5196 amod quarter,one
R1543 T5302 T5303 det no,effect
R1544 T5200 T5196 prep of,one
R1545 T5201 T5202 det the,offspring
R1546 T5202 T5200 pobj offspring,of
R1547 T5303 T5291 dobj effect,have
R1548 T5203 T5204 punct (,17
R1549 T5304 T5303 amod adverse,effect
R1550 T5305 T5303 prep on,effect
R1551 T5204 T5196 parataxis 17,one
R1552 T5306 T5307 det the,function
R1553 T5307 T5305 pobj function,on
R1554 T5308 T5307 prep of,function
R1555 T5205 T5204 prep of,17
R1556 T5309 T5310 det the,gene
R1557 T5310 T5308 pobj gene,of
R1558 T5311 T5310 compound Apc,gene
R1559 T5206 T5205 pobj 81,of
R1560 T5312 T5280 punct .,were
R1561 T5207 T5204 punct ),17
R1562 T5208 T5197 acomp homozygous,were
R1563 T5209 T5208 prep for,homozygous
R1564 T5210 T5211 det the,allele
R1565 T5211 T5209 pobj allele,for
R1566 T5212 T5211 compound ApcCKO,allele
R1567 T5213 T5197 punct .,were
R1568 T5215 T5216 det These,mice
R1569 T5216 T5217 nsubj mice,are
R1570 T5218 T5219 advmod as,as
R1571 T5219 T5216 cc as,mice
R1572 T5220 T5219 advmod well,as
R1573 T5221 T5222 amod heterozygous,mice
R1574 T5222 T5216 conj mice,mice
R1575 T5223 T5222 prep for,mice
R1576 T5224 T5225 compound ApcCKO,allele
R1577 T5225 T5223 pobj allele,for
R1578 T5226 T5217 acomp normal,are
R1579 T5227 T5217 punct ", ",are
R1580 T5228 T5217 advcl showing,are
R1581 T5229 T5230 det no,differences
R1582 T5230 T5228 dobj differences,showing
R1583 T5231 T5230 prep in,differences
R1584 T5232 T5233 poss their,survival
R1585 T5233 T5231 pobj survival,in
R1586 T5234 T5230 prep to,differences
R1587 T5235 T5236 det the,littermates
R1588 T5236 T5234 pobj littermates,to

2_test

Id Subject Object Predicate Lexical cue
17002498-11352566-85593500 448-450 11352566 denotes 12
17002498-12618378-85593501 451-453 12618378 denotes 13
17002498-11352566-85593502 700-702 11352566 denotes 12
17002498-12618378-85593503 703-705 12618378 denotes 13
17002498-15502862-85593504 3810-3812 15502862 denotes 14
T44384 448-450 11352566 denotes 12
T16891 451-453 12618378 denotes 13
T61261 700-702 11352566 denotes 12
T27200 703-705 12618378 denotes 13
T47394 3810-3812 15502862 denotes 14