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PMC:1564426 / 56646-58443 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T30401 42-48 SO_EXT:sequence_altered_entity_or_alteration_process denotes Mutant
T30402 49-53 NCBITaxon:10088 denotes mice
T30403 97-103 GO_EXT:killing denotes killed
T30404 123-126 CHEBI:16526 denotes CO2
T30405 139-143 NCBITaxon:10088 denotes Mice
T30406 171-175 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T30407 210-218 CHEBI_EXT:nitrogen denotes nitrogen
T30408 256-263 CHEBI_EXT:33893 denotes reagent
T30409 291-300 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T30410 362-370 CHEBI:75958 denotes solution
T30411 430-434 NCBITaxon:10088 denotes mice
T30412 486-493 UBERON:0000479 denotes tissues
T30413 530-539 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T30414 563-573 UBERON:0000468 denotes whole body
T30415 595-603 CHEBI:75958 denotes solution
T30416 640-646 NCBITaxon:9989 denotes Rodent
T30417 770-776 UBERON:0000479 denotes tissue
T30418 808-814 CHEBI:27338 denotes xylene
T30419 828-835 CHEBI:30879 denotes alcohol
T30420 876-887 GO_EXT:0004601 denotes peroxidases
T30421 894-898 CHEBI:16240 denotes H2O2
T30422 902-910 CHEBI:17790 denotes methanol
T30423 948-953 CHEBI:15377 denotes water
T30424 962-969 CHEBI_EXT:59132 denotes antigen
T30425 1056-1063 CHEBI_EXT:citrate denotes citrate
T30426 1064-1070 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T30427 1125-1135 GO:0042571 denotes antibodies
T30428 1167-1177 GO:0042571 denotes Antibodies
T30429 1188-1197 PR_EXT:000002198 denotes β-catenin
T30430 1202-1214 GO:0009293 denotes Transduction
T30431 1259-1269 PR_EXT:000009450 denotes keratins 1
T30432 1259-1267 _FRAGMENT denotes keratins
T30433 1271-1272 PR_EXT:000009481 denotes 5
T30434 1274-1275 PR_EXT:keratin_6 denotes 6
T30435 1277-1279 PR_EXT:000009454 denotes 14
T30436 1281-1291 PR_EXT:000009167 denotes involucrin
T30437 1293-1301 PR_EXT:000009882 denotes loricrin
T30438 1350-1359 PR_EXT:000009495 denotes keratin 8
T30439 1396-1400 PR_EXT:000010425 denotes Ki67
T30440 1466-1470 CHEBI:472552 denotes BrdU
T30441 1480-1492 MOP:0000093 denotes Biotinylated
T30442 1503-1513 GO:0042571 denotes antibodies
T30443 1515-1521 NCBITaxon:9793 denotes donkey
T30444 1527-1533 NCBITaxon:9986 denotes rabbit
T30445 1538-1542 NCBITaxon:9925 denotes goat
T30446 1548-1553 NCBITaxon:10088 denotes mouse
T30447 1554-1557 GO:0071735 denotes IgG
T30448 1785-1796 CHEBI:51686 denotes hematoxylin
R9144 T30433 T30432 _lexicallyChainedTo 5,keratins
R9145 T30434 T30432 _lexicallyChainedTo 6,keratins
R9146 T30435 T30432 _lexicallyChainedTo 14,keratins

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T30363 49-53 NCBITaxon:10088 denotes mice
T30364 123-126 CHEBI:16526 denotes CO2
T30365 139-143 NCBITaxon:10088 denotes Mice
T30366 256-263 CHEBI:33893 denotes reagent
T30367 362-370 CHEBI:75958 denotes solution
T30368 430-434 NCBITaxon:10088 denotes mice
T30369 486-493 UBERON:0000479 denotes tissues
T30370 563-573 UBERON:0000468 denotes whole body
T30371 595-603 CHEBI:75958 denotes solution
T30372 640-646 NCBITaxon:9989 denotes Rodent
T30373 770-776 UBERON:0000479 denotes tissue
T30374 808-814 CHEBI:27338 denotes xylene
T30375 828-835 CHEBI:30879 denotes alcohol
T30376 894-898 CHEBI:16240 denotes H2O2
T30377 902-910 CHEBI:17790 denotes methanol
T30378 948-953 CHEBI:15377 denotes water
T30379 962-969 CHEBI:59132 denotes antigen
T30380 1125-1135 GO:0042571 denotes antibodies
T30381 1167-1177 GO:0042571 denotes Antibodies
T30382 1188-1197 PR:000002198 denotes β-catenin
T30383 1202-1214 GO:0009293 denotes Transduction
T30384 1259-1269 PR:000009450 denotes keratins 1
T30385 1259-1267 _FRAGMENT denotes keratins
T30386 1271-1272 PR:000009481 denotes 5
T30387 1277-1279 PR:000009454 denotes 14
T30388 1281-1291 PR:000009167 denotes involucrin
T30389 1293-1301 PR:000009882 denotes loricrin
T30390 1350-1359 PR:000009495 denotes keratin 8
T30391 1396-1400 PR:000010425 denotes Ki67
T30392 1466-1470 CHEBI:472552 denotes BrdU
T30393 1480-1492 MOP:0000093 denotes Biotinylated
T30394 1503-1513 GO:0042571 denotes antibodies
T30395 1515-1521 NCBITaxon:9793 denotes donkey
T30396 1527-1533 NCBITaxon:9986 denotes rabbit
T30397 1538-1542 NCBITaxon:9925 denotes goat
T30398 1548-1553 NCBITaxon:10088 denotes mouse
T30399 1554-1557 GO:0071735 denotes IgG
T30400 1785-1796 CHEBI:51686 denotes hematoxylin
R9142 T30386 T30385 _lexicallyChainedTo 5,keratins
R9143 T30387 T30385 _lexicallyChainedTo 14,keratins

craft-sa-dev

Id Subject Object Predicate Lexical cue
T30449 0-12 JJ denotes Histological
T30450 32-40 NN denotes analysis
T30451 13-16 CC denotes and
T30452 17-31 JJ denotes immunochemical
T30453 40-41 . denotes .
T30454 41-138 sentence denotes Mutant mice and age-matching littermates were humanely killed at various ages by CO2 inhalation.
T30455 42-48 JJ denotes Mutant
T30456 49-53 NNS denotes mice
T30457 97-103 VBN denotes killed
T30458 54-57 CC denotes and
T30459 58-61 NN denotes age
T30460 62-70 JJ denotes matching
T30461 61-62 HYPH denotes -
T30462 71-82 NNS denotes littermates
T30463 83-87 VBD denotes were
T30464 88-96 RB denotes humanely
T30465 104-106 IN denotes at
T30466 107-114 JJ denotes various
T30467 115-119 NNS denotes ages
T30468 120-122 IN denotes by
T30469 123-126 NN denotes CO2
T30470 127-137 NN denotes inhalation
T30471 137-138 . denotes .
T30472 138-425 sentence denotes Mice were skinned and pieces of skin were either snap-frozen in liquid nitrogen or immediately homogenized in Trizol reagent and stored at −80 °C until molecular analysis, or fixed flat on a piece of paper towel in Bouin's solution for histological and immunohistochemical examinations.
T30473 139-143 NNS denotes Mice
T30474 149-156 VBN denotes skinned
T30475 144-148 VBD denotes were
T30476 157-160 CC denotes and
T30477 161-167 NNS denotes pieces
T30478 193-199 VBN denotes frozen
T30479 168-170 IN denotes of
T30480 171-175 NN denotes skin
T30481 176-180 VBD denotes were
T30482 181-187 CC denotes either
T30483 188-192 NN denotes snap
T30484 192-193 HYPH denotes -
T30485 200-202 IN denotes in
T30486 203-209 JJ denotes liquid
T30487 210-218 NN denotes nitrogen
T30488 219-221 CC denotes or
T30489 222-233 RB denotes immediately
T30490 234-245 VBN denotes homogenized
T30491 246-248 IN denotes in
T30492 249-255 NNP denotes Trizol
T30493 256-263 NN denotes reagent
T30494 264-267 CC denotes and
T30495 268-274 VBN denotes stored
T30496 275-277 IN denotes at
T30497 278-279 SYM denotes −
T30498 279-281 CD denotes 80
T30499 282-284 NNS denotes °C
T30500 285-290 IN denotes until
T30501 291-300 JJ denotes molecular
T30502 301-309 NN denotes analysis
T30503 309-311 , denotes ,
T30504 311-313 CC denotes or
T30505 314-319 VBN denotes fixed
T30506 320-324 JJ denotes flat
T30507 325-327 IN denotes on
T30508 328-329 DT denotes a
T30509 330-335 NN denotes piece
T30510 336-338 IN denotes of
T30511 339-344 NN denotes paper
T30512 345-350 NN denotes towel
T30513 351-353 IN denotes in
T30514 354-359 NNP denotes Bouin
T30515 362-370 NN denotes solution
T30516 359-361 POS denotes 's
T30517 371-374 IN denotes for
T30518 375-387 JJ denotes histological
T30519 412-424 NNS denotes examinations
T30520 388-391 CC denotes and
T30521 392-411 JJ denotes immunohistochemical
T30522 424-425 . denotes .
T30523 425-604 sentence denotes The mice were then dissected for gross examination, various tissues were similarly collected for future molecular analyses, and then the whole body was fixed in Bouin's solution.
T30524 426-429 DT denotes The
T30525 430-434 NNS denotes mice
T30526 445-454 VBN denotes dissected
T30527 435-439 VBD denotes were
T30528 440-444 RB denotes then
T30529 455-458 IN denotes for
T30530 459-464 JJ denotes gross
T30531 465-476 NN denotes examination
T30532 476-478 , denotes ,
T30533 478-485 JJ denotes various
T30534 486-493 NNS denotes tissues
T30535 509-518 VBN denotes collected
T30536 494-498 VBD denotes were
T30537 499-508 RB denotes similarly
T30538 519-522 IN denotes for
T30539 523-529 JJ denotes future
T30540 540-548 NNS denotes analyses
T30541 530-539 JJ denotes molecular
T30542 548-550 , denotes ,
T30543 550-553 CC denotes and
T30544 554-558 RB denotes then
T30545 578-583 VBN denotes fixed
T30546 559-562 DT denotes the
T30547 569-573 NN denotes body
T30548 563-568 JJ denotes whole
T30549 574-577 VBD denotes was
T30550 584-586 IN denotes in
T30551 587-592 NNP denotes Bouin
T30552 595-603 NN denotes solution
T30553 592-594 POS denotes 's
T30554 603-604 . denotes .
T30555 604-720 sentence denotes The samples were then submitted to Rodent Histopathological Core for processing and histopathological examinations.
T30556 605-608 DT denotes The
T30557 609-616 NNS denotes samples
T30558 627-636 VBN denotes submitted
T30559 617-621 VBD denotes were
T30560 622-626 RB denotes then
T30561 637-639 IN denotes to
T30562 640-646 NNP denotes Rodent
T30563 665-669 NNP denotes Core
T30564 647-664 NNP denotes Histopathological
T30565 670-673 IN denotes for
T30566 674-684 NN denotes processing
T30567 685-688 CC denotes and
T30568 689-706 JJ denotes histopathological
T30569 707-719 NNS denotes examinations
T30570 719-720 . denotes .
T30571 720-848 sentence denotes For immunohistochemistry, 5-μm paraffin-embedded tissue sections were deparafinized in xylene, followed by alcohol rehydration.
T30572 721-724 IN denotes For
T30573 791-804 VBN denotes deparafinized
T30574 725-745 NN denotes immunohistochemistry
T30575 745-747 , denotes ,
T30576 747-748 CD denotes 5
T30577 749-751 NNS denotes μm
T30578 748-749 HYPH denotes -
T30579 777-785 NNS denotes sections
T30580 752-760 NN denotes paraffin
T30581 761-769 VBN denotes embedded
T30582 760-761 HYPH denotes -
T30583 770-776 NN denotes tissue
T30584 786-790 VBD denotes were
T30585 805-807 IN denotes in
T30586 808-814 NN denotes xylene
T30587 814-816 , denotes ,
T30588 816-824 VBN denotes followed
T30589 825-827 IN denotes by
T30590 828-835 NN denotes alcohol
T30591 836-847 NN denotes rehydration
T30592 847-848 . denotes .
T30593 848-1080 sentence denotes After quenching endogenous peroxidases in 3% H2O2 in methanol, the slides were rinsed in distilled water, and an antigen retrieval step was carried out in a microwave oven for a total of 10 min in preheated citrate buffer (pH 6.0).
T30594 849-854 IN denotes After
T30595 928-934 VBN denotes rinsed
T30596 855-864 VBG denotes quenching
T30597 865-875 JJ denotes endogenous
T30598 876-887 NNS denotes peroxidases
T30599 888-890 IN denotes in
T30600 891-892 CD denotes 3
T30601 892-893 NN denotes %
T30602 894-898 NN denotes H2O2
T30603 899-901 IN denotes in
T30604 902-910 NN denotes methanol
T30605 910-912 , denotes ,
T30606 912-915 DT denotes the
T30607 916-922 NNS denotes slides
T30608 923-927 VBD denotes were
T30609 935-937 IN denotes in
T30610 938-947 JJ denotes distilled
T30611 948-953 NN denotes water
T30612 953-955 , denotes ,
T30613 955-958 CC denotes and
T30614 959-961 DT denotes an
T30615 980-984 NN denotes step
T30616 962-969 NN denotes antigen
T30617 970-979 NN denotes retrieval
T30618 989-996 VBN denotes carried
T30619 985-988 VBD denotes was
T30620 997-1000 RP denotes out
T30621 1001-1003 IN denotes in
T30622 1004-1005 DT denotes a
T30623 1006-1015 NN denotes microwave
T30624 1016-1020 VBN denotes oven
T30625 1021-1024 IN denotes for
T30626 1025-1026 DT denotes a
T30627 1027-1032 NN denotes total
T30628 1033-1035 IN denotes of
T30629 1036-1038 CD denotes 10
T30630 1039-1042 NNS denotes min
T30631 1043-1045 IN denotes in
T30632 1046-1055 JJ denotes preheated
T30633 1064-1070 NN denotes buffer
T30634 1056-1063 NN denotes citrate
T30635 1071-1072 -LRB- denotes (
T30636 1072-1074 NN denotes pH
T30637 1075-1078 CD denotes 6.0
T30638 1078-1079 -RRB- denotes )
T30639 1079-1080 . denotes .
T30640 1080-1166 sentence denotes The slides were then incubated with primary antibodies at room temperature overnight.
T30641 1081-1084 DT denotes The
T30642 1085-1091 NNS denotes slides
T30643 1102-1111 VBN denotes incubated
T30644 1092-1096 VBD denotes were
T30645 1097-1101 RB denotes then
T30646 1112-1116 IN denotes with
T30647 1117-1124 JJ denotes primary
T30648 1125-1135 NNS denotes antibodies
T30649 1136-1138 IN denotes at
T30650 1139-1143 NN denotes room
T30651 1144-1155 NN denotes temperature
T30652 1156-1165 RB denotes overnight
T30653 1165-1166 . denotes .
T30654 1166-1479 sentence denotes Antibodies used were β-catenin (BD Transduction Lab, San Diego, California, United States), keratins 1, 5, 6, 14, involucrin, loricrin (Covance, Berkeley, California, United States), keratin 8 (Abcam, Cambridge, United Kingdom), Ki67 (Vector Laboratories, Burlingame, California, United States) and BrdU (Roche).
T30655 1167-1177 NNS denotes Antibodies
T30656 1183-1187 VBD denotes were
T30657 1178-1182 VBN denotes used
T30658 1188-1189 NN denotes β
T30659 1190-1197 NN denotes catenin
T30660 1189-1190 HYPH denotes -
T30661 1198-1199 -LRB- denotes (
T30662 1215-1218 NNP denotes Lab
T30663 1199-1201 NNP denotes BD
T30664 1202-1214 NNP denotes Transduction
T30665 1218-1220 , denotes ,
T30666 1220-1223 NNP denotes San
T30667 1224-1229 NNP denotes Diego
T30668 1229-1231 , denotes ,
T30669 1231-1241 NNP denotes California
T30670 1241-1243 , denotes ,
T30671 1243-1249 NNP denotes United
T30672 1250-1256 NNP denotes States
T30673 1256-1257 -RRB- denotes )
T30674 1257-1259 , denotes ,
T30675 1259-1267 NNS denotes keratins
T30676 1277-1279 CD denotes 14
T30677 1268-1269 CD denotes 1
T30678 1269-1271 , denotes ,
T30679 1271-1272 CD denotes 5
T30680 1272-1274 , denotes ,
T30681 1274-1275 CD denotes 6
T30682 1275-1277 , denotes ,
T30683 1279-1281 , denotes ,
T30684 1281-1291 NN denotes involucrin
T30685 1291-1293 , denotes ,
T30686 1293-1301 NN denotes loricrin
T30687 1302-1303 -LRB- denotes (
T30688 1303-1310 NNP denotes Covance
T30689 1310-1312 , denotes ,
T30690 1312-1320 NNP denotes Berkeley
T30691 1320-1322 , denotes ,
T30692 1322-1332 NNP denotes California
T30693 1332-1334 , denotes ,
T30694 1334-1340 NNP denotes United
T30695 1341-1347 NNP denotes States
T30696 1347-1348 -RRB- denotes )
T30697 1348-1350 , denotes ,
T30698 1350-1357 NN denotes keratin
T30699 1358-1359 CD denotes 8
T30700 1360-1361 -LRB- denotes (
T30701 1361-1366 NNP denotes Abcam
T30702 1366-1368 , denotes ,
T30703 1368-1377 NNP denotes Cambridge
T30704 1377-1379 , denotes ,
T30705 1379-1385 NNP denotes United
T30706 1386-1393 NNP denotes Kingdom
T30707 1393-1394 -RRB- denotes )
T30708 1394-1396 , denotes ,
T30709 1396-1400 NN denotes Ki67
T30710 1401-1402 -LRB- denotes (
T30711 1409-1421 NNP denotes Laboratories
T30712 1402-1408 NNP denotes Vector
T30713 1421-1423 , denotes ,
T30714 1423-1433 NNP denotes Burlingame
T30715 1433-1435 , denotes ,
T30716 1435-1445 NNP denotes California
T30717 1445-1447 , denotes ,
T30718 1447-1453 NNP denotes United
T30719 1454-1460 NNP denotes States
T30720 1460-1461 -RRB- denotes )
T30721 1462-1465 CC denotes and
T30722 1466-1470 NN denotes BrdU
T30723 1471-1472 -LRB- denotes (
T30724 1472-1477 NNP denotes Roche
T30725 1477-1478 -RRB- denotes )
T30726 1478-1479 . denotes .
T30727 1479-1719 sentence denotes Biotinylated secondary antibodies (donkey anti-rabbit and goat anti-mouse IgG, 1:250; Jackson ImmunoResearch, West Grove, Pennsylvania, United States), followed by the Vectastain Elite ABC kit (Vector Laboratories) were used for detection.
T30728 1480-1492 VBN denotes Biotinylated
T30729 1503-1513 NNS denotes antibodies
T30730 1493-1502 JJ denotes secondary
T30731 1700-1704 VBN denotes used
T30732 1514-1515 -LRB- denotes (
T30733 1574-1588 NNP denotes ImmunoResearch
T30734 1515-1521 NN denotes donkey
T30735 1522-1533 JJ denotes anti-rabbit
T30736 1554-1557 NN denotes IgG
T30737 1534-1537 CC denotes and
T30738 1538-1542 NN denotes goat
T30739 1543-1553 JJ denotes anti-mouse
T30740 1557-1559 , denotes ,
T30741 1559-1560 CD denotes 1
T30742 1560-1561 SYM denotes :
T30743 1561-1564 CD denotes 250
T30744 1564-1565 : denotes ;
T30745 1566-1573 NNP denotes Jackson
T30746 1588-1590 , denotes ,
T30747 1590-1594 NNP denotes West
T30748 1595-1600 NNP denotes Grove
T30749 1600-1602 , denotes ,
T30750 1602-1614 NNP denotes Pennsylvania
T30751 1614-1616 , denotes ,
T30752 1616-1622 NNP denotes United
T30753 1623-1629 NNP denotes States
T30754 1629-1630 -RRB- denotes )
T30755 1630-1632 , denotes ,
T30756 1632-1640 VBN denotes followed
T30757 1641-1643 IN denotes by
T30758 1644-1647 DT denotes the
T30759 1669-1672 NN denotes kit
T30760 1648-1658 NNP denotes Vectastain
T30761 1659-1664 NNP denotes Elite
T30762 1665-1668 NNP denotes ABC
T30763 1673-1674 -LRB- denotes (
T30764 1681-1693 NNP denotes Laboratories
T30765 1674-1680 NNP denotes Vector
T30766 1693-1694 -RRB- denotes )
T30767 1695-1699 VBD denotes were
T30768 1705-1708 IN denotes for
T30769 1709-1718 NN denotes detection
T30770 1718-1719 . denotes .
T30771 1719-1797 sentence denotes The slides were stained with DAB and counterstained with Mayer's hematoxylin.
T30772 1720-1723 DT denotes The
T30773 1724-1730 NNS denotes slides
T30774 1736-1743 VBN denotes stained
T30775 1731-1735 VBD denotes were
T30776 1744-1748 IN denotes with
T30777 1749-1752 NN denotes DAB
T30778 1753-1756 CC denotes and
T30779 1757-1771 VBN denotes counterstained
T30780 1772-1776 IN denotes with
T30781 1777-1782 NNP denotes Mayer
T30782 1785-1796 NN denotes hematoxylin
T30783 1782-1784 POS denotes 's
T30784 1796-1797 . denotes .
R9147 T30449 T30450 amod Histological,analysis
R9148 T30451 T30449 cc and,Histological
R9149 T30452 T30449 conj immunochemical,Histological
R9150 T30453 T30450 punct .,analysis
R9151 T30455 T30456 amod Mutant,mice
R9152 T30456 T30457 nsubjpass mice,killed
R9153 T30458 T30456 cc and,mice
R9154 T30459 T30460 npadvmod age,matching
R9155 T30460 T30462 amod matching,littermates
R9156 T30461 T30460 punct -,matching
R9157 T30462 T30456 conj littermates,mice
R9158 T30463 T30457 auxpass were,killed
R9159 T30464 T30457 advmod humanely,killed
R9160 T30465 T30457 prep at,killed
R9161 T30466 T30467 amod various,ages
R9162 T30467 T30465 pobj ages,at
R9163 T30468 T30457 prep by,killed
R9164 T30469 T30470 compound CO2,inhalation
R9165 T30470 T30468 pobj inhalation,by
R9166 T30471 T30457 punct .,killed
R9167 T30473 T30474 nsubjpass Mice,skinned
R9168 T30475 T30474 auxpass were,skinned
R9169 T30476 T30474 cc and,skinned
R9170 T30477 T30478 nsubj pieces,frozen
R9171 T30478 T30474 conj frozen,skinned
R9172 T30479 T30477 prep of,pieces
R9173 T30480 T30479 pobj skin,of
R9174 T30481 T30478 aux were,frozen
R9175 T30482 T30478 preconj either,frozen
R9176 T30483 T30478 dep snap,frozen
R9177 T30484 T30478 punct -,frozen
R9178 T30485 T30478 prep in,frozen
R9179 T30486 T30487 amod liquid,nitrogen
R9180 T30487 T30485 pobj nitrogen,in
R9181 T30488 T30478 cc or,frozen
R9182 T30489 T30490 advmod immediately,homogenized
R9183 T30490 T30478 conj homogenized,frozen
R9184 T30491 T30490 prep in,homogenized
R9185 T30492 T30493 compound Trizol,reagent
R9186 T30493 T30491 pobj reagent,in
R9187 T30494 T30478 cc and,frozen
R9188 T30495 T30478 conj stored,frozen
R9189 T30496 T30495 prep at,stored
R9190 T30497 T30498 punct −,80
R9191 T30498 T30499 nummod 80,°C
R9192 T30499 T30496 pobj °C,at
R9193 T30500 T30495 prep until,stored
R9194 T30501 T30502 amod molecular,analysis
R9195 T30502 T30500 pobj analysis,until
R9196 T30503 T30495 punct ", ",stored
R9197 T30504 T30495 cc or,stored
R9198 T30505 T30495 conj fixed,stored
R9199 T30506 T30505 oprd flat,fixed
R9200 T30507 T30505 prep on,fixed
R9201 T30508 T30509 det a,piece
R9202 T30509 T30507 pobj piece,on
R9203 T30510 T30509 prep of,piece
R9204 T30511 T30512 compound paper,towel
R9205 T30512 T30510 pobj towel,of
R9206 T30513 T30505 prep in,fixed
R9207 T30514 T30515 poss Bouin,solution
R9208 T30515 T30513 pobj solution,in
R9209 T30516 T30514 case 's,Bouin
R9210 T30517 T30505 prep for,fixed
R9211 T30518 T30519 amod histological,examinations
R9212 T30519 T30517 pobj examinations,for
R9213 T30520 T30518 cc and,histological
R9214 T30521 T30518 conj immunohistochemical,histological
R9215 T30522 T30478 punct .,frozen
R9216 T30524 T30525 det The,mice
R9217 T30525 T30526 nsubjpass mice,dissected
R9218 T30527 T30526 auxpass were,dissected
R9219 T30528 T30526 advmod then,dissected
R9220 T30529 T30526 prep for,dissected
R9221 T30530 T30531 amod gross,examination
R9222 T30531 T30529 pobj examination,for
R9223 T30532 T30526 punct ", ",dissected
R9224 T30533 T30534 amod various,tissues
R9225 T30534 T30535 nsubjpass tissues,collected
R9226 T30535 T30526 conj collected,dissected
R9227 T30536 T30535 auxpass were,collected
R9228 T30537 T30535 advmod similarly,collected
R9229 T30538 T30535 prep for,collected
R9230 T30539 T30540 amod future,analyses
R9231 T30540 T30538 pobj analyses,for
R9232 T30541 T30540 amod molecular,analyses
R9233 T30542 T30535 punct ", ",collected
R9234 T30543 T30535 cc and,collected
R9235 T30544 T30545 advmod then,fixed
R9236 T30545 T30535 conj fixed,collected
R9237 T30546 T30547 det the,body
R9238 T30547 T30545 nsubjpass body,fixed
R9239 T30548 T30547 amod whole,body
R9240 T30549 T30545 auxpass was,fixed
R9241 T30550 T30545 prep in,fixed
R9242 T30551 T30552 poss Bouin,solution
R9243 T30552 T30550 pobj solution,in
R9244 T30553 T30551 case 's,Bouin
R9245 T30554 T30545 punct .,fixed
R9246 T30556 T30557 det The,samples
R9247 T30557 T30558 nsubjpass samples,submitted
R9248 T30559 T30558 auxpass were,submitted
R9249 T30560 T30558 advmod then,submitted
R9250 T30561 T30558 prep to,submitted
R9251 T30562 T30563 compound Rodent,Core
R9252 T30563 T30561 pobj Core,to
R9253 T30564 T30563 compound Histopathological,Core
R9254 T30565 T30558 prep for,submitted
R9255 T30566 T30565 pobj processing,for
R9256 T30567 T30566 cc and,processing
R9257 T30568 T30569 amod histopathological,examinations
R9258 T30569 T30566 conj examinations,processing
R9259 T30570 T30558 punct .,submitted
R9260 T30572 T30573 prep For,deparafinized
R9261 T30574 T30572 pobj immunohistochemistry,For
R9262 T30575 T30573 punct ", ",deparafinized
R9263 T30576 T30577 nummod 5,μm
R9264 T30577 T30579 nmod μm,sections
R9265 T30578 T30577 punct -,μm
R9266 T30579 T30573 nsubjpass sections,deparafinized
R9267 T30580 T30581 npadvmod paraffin,embedded
R9268 T30581 T30579 amod embedded,sections
R9269 T30582 T30581 punct -,embedded
R9270 T30583 T30579 compound tissue,sections
R9271 T30584 T30573 auxpass were,deparafinized
R9272 T30585 T30573 prep in,deparafinized
R9273 T30586 T30585 pobj xylene,in
R9274 T30587 T30573 punct ", ",deparafinized
R9275 T30588 T30573 advcl followed,deparafinized
R9276 T30589 T30588 agent by,followed
R9277 T30590 T30591 compound alcohol,rehydration
R9278 T30591 T30589 pobj rehydration,by
R9279 T30592 T30573 punct .,deparafinized
R9280 T30594 T30595 prep After,rinsed
R9281 T30596 T30594 pcomp quenching,After
R9282 T30597 T30598 amod endogenous,peroxidases
R9283 T30598 T30596 dobj peroxidases,quenching
R9284 T30599 T30596 prep in,quenching
R9285 T30600 T30601 nummod 3,%
R9286 T30601 T30602 compound %,H2O2
R9287 T30602 T30599 pobj H2O2,in
R9288 T30603 T30596 prep in,quenching
R9289 T30604 T30603 pobj methanol,in
R9290 T30605 T30595 punct ", ",rinsed
R9291 T30606 T30607 det the,slides
R9292 T30607 T30595 nsubjpass slides,rinsed
R9293 T30608 T30595 auxpass were,rinsed
R9294 T30609 T30595 prep in,rinsed
R9295 T30610 T30611 amod distilled,water
R9296 T30611 T30609 pobj water,in
R9297 T30612 T30595 punct ", ",rinsed
R9298 T30613 T30595 cc and,rinsed
R9299 T30614 T30615 det an,step
R9300 T30615 T30618 nsubjpass step,carried
R9301 T30616 T30615 compound antigen,step
R9302 T30617 T30615 compound retrieval,step
R9303 T30618 T30595 conj carried,rinsed
R9304 T30619 T30618 auxpass was,carried
R9305 T30620 T30618 prt out,carried
R9306 T30621 T30618 prep in,carried
R9307 T30622 T30623 det a,microwave
R9308 T30623 T30621 pobj microwave,in
R9309 T30624 T30623 amod oven,microwave
R9310 T30625 T30618 prep for,carried
R9311 T30626 T30627 det a,total
R9312 T30627 T30625 pobj total,for
R9313 T30628 T30627 prep of,total
R9314 T30629 T30630 nummod 10,min
R9315 T30630 T30628 pobj min,of
R9316 T30631 T30618 prep in,carried
R9317 T30632 T30633 amod preheated,buffer
R9318 T30633 T30631 pobj buffer,in
R9319 T30634 T30633 compound citrate,buffer
R9320 T30635 T30636 punct (,pH
R9321 T30636 T30633 parataxis pH,buffer
R9322 T30637 T30636 nummod 6.0,pH
R9323 T30638 T30636 punct ),pH
R9324 T30639 T30595 punct .,rinsed
R9325 T30641 T30642 det The,slides
R9326 T30642 T30643 nsubjpass slides,incubated
R9327 T30644 T30643 auxpass were,incubated
R9328 T30645 T30643 advmod then,incubated
R9329 T30646 T30643 prep with,incubated
R9330 T30647 T30648 amod primary,antibodies
R9331 T30648 T30646 pobj antibodies,with
R9332 T30649 T30643 prep at,incubated
R9333 T30650 T30651 compound room,temperature
R9334 T30651 T30649 pobj temperature,at
R9335 T30652 T30643 advmod overnight,incubated
R9336 T30653 T30643 punct .,incubated
R9337 T30655 T30656 nsubj Antibodies,were
R9338 T30657 T30655 acl used,Antibodies
R9339 T30658 T30659 compound β,catenin
R9340 T30659 T30656 attr catenin,were
R9341 T30660 T30659 punct -,catenin
R9342 T30661 T30662 punct (,Lab
R9343 T30662 T30659 parataxis Lab,catenin
R9344 T30663 T30662 compound BD,Lab
R9345 T30664 T30662 compound Transduction,Lab
R9346 T30665 T30662 punct ", ",Lab
R9347 T30666 T30667 compound San,Diego
R9348 T30667 T30662 npadvmod Diego,Lab
R9349 T30668 T30662 punct ", ",Lab
R9350 T30669 T30662 npadvmod California,Lab
R9351 T30670 T30662 punct ", ",Lab
R9352 T30671 T30672 compound United,States
R9353 T30672 T30662 npadvmod States,Lab
R9354 T30673 T30662 punct ),Lab
R9355 T30674 T30659 punct ", ",catenin
R9356 T30675 T30676 nmod keratins,14
R9357 T30676 T30659 conj 14,catenin
R9358 T30677 T30676 nummod 1,14
R9359 T30678 T30676 punct ", ",14
R9360 T30679 T30676 nummod 5,14
R9361 T30680 T30676 punct ", ",14
R9362 T30681 T30676 nummod 6,14
R9363 T30682 T30676 punct ", ",14
R9364 T30683 T30676 punct ", ",14
R9365 T30684 T30676 conj involucrin,14
R9366 T30685 T30684 punct ", ",involucrin
R9367 T30686 T30684 conj loricrin,involucrin
R9368 T30687 T30688 punct (,Covance
R9369 T30688 T30686 parataxis Covance,loricrin
R9370 T30689 T30688 punct ", ",Covance
R9371 T30690 T30688 npadvmod Berkeley,Covance
R9372 T30691 T30688 punct ", ",Covance
R9373 T30692 T30688 npadvmod California,Covance
R9374 T30693 T30688 punct ", ",Covance
R9375 T30694 T30695 compound United,States
R9376 T30695 T30688 npadvmod States,Covance
R9377 T30696 T30688 punct ),Covance
R9378 T30697 T30686 punct ", ",loricrin
R9379 T30698 T30686 conj keratin,loricrin
R9380 T30699 T30698 nummod 8,keratin
R9381 T30700 T30701 punct (,Abcam
R9382 T30701 T30698 parataxis Abcam,keratin
R9383 T30702 T30701 punct ", ",Abcam
R9384 T30703 T30701 npadvmod Cambridge,Abcam
R9385 T30704 T30701 punct ", ",Abcam
R9386 T30705 T30706 compound United,Kingdom
R9387 T30706 T30701 npadvmod Kingdom,Abcam
R9388 T30707 T30701 punct ),Abcam
R9389 T30708 T30698 punct ", ",keratin
R9390 T30709 T30698 conj Ki67,keratin
R9391 T30710 T30711 punct (,Laboratories
R9392 T30711 T30709 parataxis Laboratories,Ki67
R9393 T30712 T30711 compound Vector,Laboratories
R9394 T30713 T30711 punct ", ",Laboratories
R9395 T30714 T30711 npadvmod Burlingame,Laboratories
R9396 T30715 T30711 punct ", ",Laboratories
R9397 T30716 T30711 npadvmod California,Laboratories
R9398 T30717 T30711 punct ", ",Laboratories
R9399 T30718 T30719 compound United,States
R9400 T30719 T30711 npadvmod States,Laboratories
R9401 T30720 T30711 punct ),Laboratories
R9402 T30721 T30709 cc and,Ki67
R9403 T30722 T30709 conj BrdU,Ki67
R9404 T30723 T30724 punct (,Roche
R9405 T30724 T30722 parataxis Roche,BrdU
R9406 T30725 T30724 punct ),Roche
R9407 T30726 T30656 punct .,were
R9408 T30728 T30729 amod Biotinylated,antibodies
R9409 T30729 T30731 nsubjpass antibodies,used
R9410 T30730 T30729 amod secondary,antibodies
R9411 T30732 T30733 punct (,ImmunoResearch
R9412 T30733 T30729 parataxis ImmunoResearch,antibodies
R9413 T30734 T30735 npadvmod donkey,anti-rabbit
R9414 T30735 T30736 amod anti-rabbit,IgG
R9415 T30736 T30733 dep IgG,ImmunoResearch
R9416 T30737 T30735 cc and,anti-rabbit
R9417 T30738 T30739 npadvmod goat,anti-mouse
R9418 T30739 T30735 conj anti-mouse,anti-rabbit
R9419 T30740 T30736 punct ", ",IgG
R9420 T30741 T30736 npadvmod 1,IgG
R9421 T30742 T30743 punct :,250
R9422 T30743 T30741 prep 250,1
R9423 T30744 T30733 punct ;,ImmunoResearch
R9424 T30745 T30733 compound Jackson,ImmunoResearch
R9425 T30746 T30733 punct ", ",ImmunoResearch
R9426 T30747 T30748 compound West,Grove
R9427 T30748 T30733 npadvmod Grove,ImmunoResearch
R9428 T30749 T30733 punct ", ",ImmunoResearch
R9429 T30750 T30733 npadvmod Pennsylvania,ImmunoResearch
R9430 T30751 T30733 punct ", ",ImmunoResearch
R9431 T30752 T30753 compound United,States
R9432 T30753 T30733 npadvmod States,ImmunoResearch
R9433 T30754 T30733 punct ),ImmunoResearch
R9434 T30755 T30729 punct ", ",antibodies
R9435 T30756 T30729 acl followed,antibodies
R9436 T30757 T30756 agent by,followed
R9437 T30758 T30759 det the,kit
R9438 T30759 T30757 pobj kit,by
R9439 T30760 T30759 compound Vectastain,kit
R9440 T30761 T30759 compound Elite,kit
R9441 T30762 T30759 compound ABC,kit
R9442 T30763 T30764 punct (,Laboratories
R9443 T30764 T30759 parataxis Laboratories,kit
R9444 T30765 T30764 compound Vector,Laboratories
R9445 T30766 T30764 punct ),Laboratories
R9446 T30767 T30731 auxpass were,used
R9447 T30768 T30731 prep for,used
R9448 T30769 T30768 pobj detection,for
R9449 T30770 T30731 punct .,used
R9450 T30772 T30773 det The,slides
R9451 T30773 T30774 nsubjpass slides,stained
R9452 T30775 T30774 auxpass were,stained
R9453 T30776 T30774 prep with,stained
R9454 T30777 T30776 pobj DAB,with
R9455 T30778 T30774 cc and,stained
R9456 T30779 T30774 conj counterstained,stained
R9457 T30780 T30779 prep with,counterstained
R9458 T30781 T30782 poss Mayer,hematoxylin
R9459 T30782 T30780 pobj hematoxylin,with
R9460 T30783 T30781 case 's,Mayer
R9461 T30784 T30774 punct .,stained