Id |
Subject |
Object |
Predicate |
Lexical cue |
T29508 |
0-8 |
NN |
denotes |
Genotype |
T29509 |
9-15 |
NN |
denotes |
tissue |
T29510 |
8-9 |
HYPH |
denotes |
/ |
T29511 |
16-24 |
JJ |
denotes |
specific |
T29512 |
15-16 |
HYPH |
denotes |
- |
T29513 |
25-38 |
NN |
denotes |
recombination |
T29514 |
69-77 |
VBN |
denotes |
examined |
T29515 |
39-41 |
IN |
denotes |
of |
T29516 |
42-45 |
DT |
denotes |
the |
T29517 |
58-64 |
NN |
denotes |
allele |
T29518 |
46-57 |
JJ |
denotes |
conditional |
T29519 |
65-68 |
VBD |
denotes |
was |
T29520 |
78-80 |
IN |
denotes |
by |
T29521 |
81-85 |
CC |
denotes |
both |
T29522 |
86-89 |
NN |
denotes |
PCR |
T29523 |
90-93 |
CC |
denotes |
and |
T29524 |
94-96 |
NN |
denotes |
RT |
T29525 |
97-100 |
NN |
denotes |
PCR |
T29526 |
96-97 |
HYPH |
denotes |
- |
T29527 |
100-101 |
. |
denotes |
. |
T29528 |
101-223 |
sentence |
denotes |
DNA samples extracted from various tissues collected at the time of autopsy were examined by genotyping PCR as described. |
T29529 |
102-105 |
NN |
denotes |
DNA |
T29530 |
106-113 |
NNS |
denotes |
samples |
T29531 |
183-191 |
VBN |
denotes |
examined |
T29532 |
114-123 |
VBN |
denotes |
extracted |
T29533 |
124-128 |
IN |
denotes |
from |
T29534 |
129-136 |
JJ |
denotes |
various |
T29535 |
137-144 |
NNS |
denotes |
tissues |
T29536 |
145-154 |
VBN |
denotes |
collected |
T29537 |
155-157 |
IN |
denotes |
at |
T29538 |
158-161 |
DT |
denotes |
the |
T29539 |
162-166 |
NN |
denotes |
time |
T29540 |
167-169 |
IN |
denotes |
of |
T29541 |
170-177 |
NN |
denotes |
autopsy |
T29542 |
178-182 |
VBD |
denotes |
were |
T29543 |
192-194 |
IN |
denotes |
by |
T29544 |
195-205 |
VBG |
denotes |
genotyping |
T29545 |
206-209 |
NN |
denotes |
PCR |
T29546 |
210-212 |
IN |
denotes |
as |
T29547 |
213-222 |
VBN |
denotes |
described |
T29548 |
222-223 |
. |
denotes |
. |
T29549 |
223-490 |
sentence |
denotes |
RNA extracted from various tissue homogenates in Trizol reagent were examined for expression of wild-type and truncated Apc alleles by SuperScript One-Step RT-PCR with Platinum Taq (Invitrogen, Carlsbad, California, United States), following manufacturer's protocol. |
T29550 |
224-227 |
NN |
denotes |
RNA |
T29551 |
293-301 |
VBN |
denotes |
examined |
T29552 |
228-237 |
VBN |
denotes |
extracted |
T29553 |
238-242 |
IN |
denotes |
from |
T29554 |
243-250 |
JJ |
denotes |
various |
T29555 |
258-269 |
NNS |
denotes |
homogenates |
T29556 |
251-257 |
NN |
denotes |
tissue |
T29557 |
270-272 |
IN |
denotes |
in |
T29558 |
273-279 |
NN |
denotes |
Trizol |
T29559 |
280-287 |
NN |
denotes |
reagent |
T29560 |
288-292 |
VBD |
denotes |
were |
T29561 |
302-305 |
IN |
denotes |
for |
T29562 |
306-316 |
NN |
denotes |
expression |
T29563 |
317-319 |
IN |
denotes |
of |
T29564 |
320-324 |
JJ |
denotes |
wild |
T29565 |
325-329 |
NN |
denotes |
type |
T29566 |
324-325 |
HYPH |
denotes |
- |
T29567 |
348-355 |
NNS |
denotes |
alleles |
T29568 |
330-333 |
CC |
denotes |
and |
T29569 |
334-343 |
VBN |
denotes |
truncated |
T29570 |
344-347 |
NN |
denotes |
Apc |
T29571 |
356-358 |
IN |
denotes |
by |
T29572 |
359-370 |
NNP |
denotes |
SuperScript |
T29573 |
383-386 |
NN |
denotes |
PCR |
T29574 |
371-374 |
CD |
denotes |
One |
T29575 |
375-379 |
NNP |
denotes |
Step |
T29576 |
374-375 |
HYPH |
denotes |
- |
T29577 |
380-382 |
NN |
denotes |
RT |
T29578 |
382-383 |
HYPH |
denotes |
- |
T29579 |
387-391 |
IN |
denotes |
with |
T29580 |
392-400 |
NN |
denotes |
Platinum |
T29581 |
401-404 |
NN |
denotes |
Taq |
T29582 |
405-406 |
-LRB- |
denotes |
( |
T29583 |
406-416 |
NNP |
denotes |
Invitrogen |
T29584 |
416-418 |
, |
denotes |
, |
T29585 |
418-426 |
NNP |
denotes |
Carlsbad |
T29586 |
426-428 |
, |
denotes |
, |
T29587 |
428-438 |
NNP |
denotes |
California |
T29588 |
438-440 |
, |
denotes |
, |
T29589 |
440-446 |
NNP |
denotes |
United |
T29590 |
447-453 |
NNP |
denotes |
States |
T29591 |
453-454 |
-RRB- |
denotes |
) |
T29592 |
454-456 |
, |
denotes |
, |
T29593 |
456-465 |
VBG |
denotes |
following |
T29594 |
466-478 |
NN |
denotes |
manufacturer |
T29595 |
481-489 |
NN |
denotes |
protocol |
T29596 |
478-480 |
POS |
denotes |
's |
T29597 |
489-490 |
. |
denotes |
. |
T29598 |
490-795 |
sentence |
denotes |
Approximately 200 ng of total RNA from either skin, liver, or thymus from each genotype was reverse-transcribed using primers Apc-F546 (TGAGGAATTTGTCTTGGCGAG) and Apc-R721 (GCACTTCCCATGGCAATCATT), resulting in 528-bp and 313-bp products from the wild-type/ApcCKO alleles and ApcΔ580 allele, respectively. |
T29599 |
491-504 |
RB |
denotes |
Approximately |
T29600 |
509-511 |
NNS |
denotes |
ng |
T29601 |
505-508 |
CD |
denotes |
200 |
T29602 |
591-602 |
VBN |
denotes |
transcribed |
T29603 |
512-514 |
IN |
denotes |
of |
T29604 |
515-520 |
JJ |
denotes |
total |
T29605 |
521-524 |
NN |
denotes |
RNA |
T29606 |
525-529 |
IN |
denotes |
from |
T29607 |
530-536 |
DT |
denotes |
either |
T29608 |
537-541 |
NN |
denotes |
skin |
T29609 |
541-543 |
, |
denotes |
, |
T29610 |
543-548 |
NN |
denotes |
liver |
T29611 |
548-550 |
, |
denotes |
, |
T29612 |
550-552 |
CC |
denotes |
or |
T29613 |
553-559 |
NN |
denotes |
thymus |
T29614 |
560-564 |
IN |
denotes |
from |
T29615 |
565-569 |
DT |
denotes |
each |
T29616 |
570-578 |
NN |
denotes |
genotype |
T29617 |
579-582 |
VBD |
denotes |
was |
T29618 |
583-590 |
RB |
denotes |
reverse |
T29619 |
590-591 |
HYPH |
denotes |
- |
T29620 |
603-608 |
VBG |
denotes |
using |
T29621 |
609-616 |
NNS |
denotes |
primers |
T29622 |
617-620 |
NN |
denotes |
Apc |
T29623 |
621-625 |
NN |
denotes |
F546 |
T29624 |
620-621 |
HYPH |
denotes |
- |
T29625 |
626-627 |
-LRB- |
denotes |
( |
T29626 |
627-648 |
NN |
denotes |
TGAGGAATTTGTCTTGGCGAG |
T29627 |
648-649 |
-RRB- |
denotes |
) |
T29628 |
650-653 |
CC |
denotes |
and |
T29629 |
654-657 |
NN |
denotes |
Apc |
T29630 |
658-662 |
NN |
denotes |
R721 |
T29631 |
657-658 |
HYPH |
denotes |
- |
T29632 |
663-664 |
-LRB- |
denotes |
( |
T29633 |
664-685 |
NN |
denotes |
GCACTTCCCATGGCAATCATT |
T29634 |
685-686 |
-RRB- |
denotes |
) |
T29635 |
686-688 |
, |
denotes |
, |
T29636 |
688-697 |
VBG |
denotes |
resulting |
T29637 |
698-700 |
IN |
denotes |
in |
T29638 |
701-704 |
CD |
denotes |
528 |
T29639 |
705-707 |
NNS |
denotes |
bp |
T29640 |
704-705 |
HYPH |
denotes |
- |
T29641 |
719-727 |
NNS |
denotes |
products |
T29642 |
708-711 |
CC |
denotes |
and |
T29643 |
712-715 |
CD |
denotes |
313 |
T29644 |
716-718 |
NNS |
denotes |
bp |
T29645 |
715-716 |
HYPH |
denotes |
- |
T29646 |
728-732 |
IN |
denotes |
from |
T29647 |
733-736 |
DT |
denotes |
the |
T29648 |
754-761 |
NNS |
denotes |
alleles |
T29649 |
737-741 |
JJ |
denotes |
wild |
T29650 |
742-746 |
NN |
denotes |
type |
T29651 |
741-742 |
HYPH |
denotes |
- |
T29652 |
746-747 |
HYPH |
denotes |
/ |
T29653 |
747-753 |
NN |
denotes |
ApcCKO |
T29654 |
762-765 |
CC |
denotes |
and |
T29655 |
766-773 |
NN |
denotes |
ApcΔ580 |
T29656 |
774-780 |
NN |
denotes |
allele |
T29657 |
780-782 |
, |
denotes |
, |
T29658 |
782-794 |
RB |
denotes |
respectively |
T29659 |
794-795 |
. |
denotes |
. |
R8958 |
T29508 |
T29509 |
compound |
Genotype,tissue |
R8959 |
T29509 |
T29511 |
npadvmod |
tissue,specific |
R8960 |
T29510 |
T29509 |
punct |
/,tissue |
R8961 |
T29511 |
T29513 |
amod |
specific,recombination |
R8962 |
T29512 |
T29511 |
punct |
-,specific |
R8963 |
T29513 |
T29514 |
nsubjpass |
recombination,examined |
R8964 |
T29515 |
T29513 |
prep |
of,recombination |
R8965 |
T29516 |
T29517 |
det |
the,allele |
R8966 |
T29517 |
T29515 |
pobj |
allele,of |
R8967 |
T29518 |
T29517 |
amod |
conditional,allele |
R8968 |
T29519 |
T29514 |
auxpass |
was,examined |
R8969 |
T29520 |
T29514 |
prep |
by,examined |
R8970 |
T29521 |
T29522 |
preconj |
both,PCR |
R8971 |
T29522 |
T29520 |
pobj |
PCR,by |
R8972 |
T29523 |
T29522 |
cc |
and,PCR |
R8973 |
T29524 |
T29525 |
compound |
RT,PCR |
R8974 |
T29525 |
T29522 |
conj |
PCR,PCR |
R8975 |
T29526 |
T29525 |
punct |
-,PCR |
R8976 |
T29527 |
T29514 |
punct |
.,examined |
R8977 |
T29529 |
T29530 |
compound |
DNA,samples |
R8978 |
T29530 |
T29531 |
nsubjpass |
samples,examined |
R8979 |
T29532 |
T29530 |
acl |
extracted,samples |
R8980 |
T29533 |
T29532 |
prep |
from,extracted |
R8981 |
T29534 |
T29535 |
amod |
various,tissues |
R8982 |
T29535 |
T29533 |
pobj |
tissues,from |
R8983 |
T29536 |
T29535 |
acl |
collected,tissues |
R8984 |
T29537 |
T29536 |
prep |
at,collected |
R8985 |
T29538 |
T29539 |
det |
the,time |
R8986 |
T29539 |
T29537 |
pobj |
time,at |
R8987 |
T29540 |
T29539 |
prep |
of,time |
R8988 |
T29541 |
T29540 |
pobj |
autopsy,of |
R8989 |
T29542 |
T29531 |
auxpass |
were,examined |
R8990 |
T29543 |
T29531 |
prep |
by,examined |
R8991 |
T29544 |
T29543 |
pcomp |
genotyping,by |
R8992 |
T29545 |
T29544 |
dobj |
PCR,genotyping |
R8993 |
T29546 |
T29547 |
mark |
as,described |
R8994 |
T29547 |
T29544 |
advcl |
described,genotyping |
R8995 |
T29548 |
T29531 |
punct |
.,examined |
R8996 |
T29550 |
T29551 |
nsubjpass |
RNA,examined |
R8997 |
T29552 |
T29550 |
acl |
extracted,RNA |
R8998 |
T29553 |
T29552 |
prep |
from,extracted |
R8999 |
T29554 |
T29555 |
amod |
various,homogenates |
R9000 |
T29555 |
T29553 |
pobj |
homogenates,from |
R9001 |
T29556 |
T29555 |
compound |
tissue,homogenates |
R9002 |
T29557 |
T29555 |
prep |
in,homogenates |
R9003 |
T29558 |
T29559 |
compound |
Trizol,reagent |
R9004 |
T29559 |
T29557 |
pobj |
reagent,in |
R9005 |
T29560 |
T29551 |
auxpass |
were,examined |
R9006 |
T29561 |
T29551 |
prep |
for,examined |
R9007 |
T29562 |
T29561 |
pobj |
expression,for |
R9008 |
T29563 |
T29562 |
prep |
of,expression |
R9009 |
T29564 |
T29565 |
amod |
wild,type |
R9010 |
T29565 |
T29567 |
nmod |
type,alleles |
R9011 |
T29566 |
T29565 |
punct |
-,type |
R9012 |
T29567 |
T29563 |
pobj |
alleles,of |
R9013 |
T29568 |
T29565 |
cc |
and,type |
R9014 |
T29569 |
T29570 |
amod |
truncated,Apc |
R9015 |
T29570 |
T29565 |
conj |
Apc,type |
R9016 |
T29571 |
T29551 |
prep |
by,examined |
R9017 |
T29572 |
T29573 |
nmod |
SuperScript,PCR |
R9018 |
T29573 |
T29571 |
pobj |
PCR,by |
R9019 |
T29574 |
T29575 |
nummod |
One,Step |
R9020 |
T29575 |
T29573 |
compound |
Step,PCR |
R9021 |
T29576 |
T29575 |
punct |
-,Step |
R9022 |
T29577 |
T29573 |
compound |
RT,PCR |
R9023 |
T29578 |
T29573 |
punct |
-,PCR |
R9024 |
T29579 |
T29573 |
prep |
with,PCR |
R9025 |
T29580 |
T29581 |
compound |
Platinum,Taq |
R9026 |
T29581 |
T29579 |
pobj |
Taq,with |
R9027 |
T29582 |
T29583 |
punct |
(,Invitrogen |
R9028 |
T29583 |
T29573 |
parataxis |
Invitrogen,PCR |
R9029 |
T29584 |
T29583 |
punct |
", ",Invitrogen |
R9030 |
T29585 |
T29583 |
npadvmod |
Carlsbad,Invitrogen |
R9031 |
T29586 |
T29583 |
punct |
", ",Invitrogen |
R9032 |
T29587 |
T29583 |
npadvmod |
California,Invitrogen |
R9033 |
T29588 |
T29583 |
punct |
", ",Invitrogen |
R9034 |
T29589 |
T29590 |
compound |
United,States |
R9035 |
T29590 |
T29583 |
npadvmod |
States,Invitrogen |
R9036 |
T29591 |
T29583 |
punct |
),Invitrogen |
R9037 |
T29592 |
T29551 |
punct |
", ",examined |
R9038 |
T29593 |
T29551 |
advcl |
following,examined |
R9039 |
T29594 |
T29595 |
poss |
manufacturer,protocol |
R9040 |
T29595 |
T29593 |
dobj |
protocol,following |
R9041 |
T29596 |
T29594 |
case |
's,manufacturer |
R9042 |
T29597 |
T29551 |
punct |
.,examined |
R9043 |
T29599 |
T29600 |
advmod |
Approximately,ng |
R9044 |
T29600 |
T29602 |
nsubjpass |
ng,transcribed |
R9045 |
T29601 |
T29600 |
nummod |
200,ng |
R9046 |
T29603 |
T29600 |
prep |
of,ng |
R9047 |
T29604 |
T29605 |
amod |
total,RNA |
R9048 |
T29605 |
T29603 |
pobj |
RNA,of |
R9049 |
T29606 |
T29605 |
prep |
from,RNA |
R9050 |
T29607 |
T29608 |
det |
either,skin |
R9051 |
T29608 |
T29606 |
pobj |
skin,from |
R9052 |
T29609 |
T29608 |
punct |
", ",skin |
R9053 |
T29610 |
T29608 |
conj |
liver,skin |
R9054 |
T29611 |
T29610 |
punct |
", ",liver |
R9055 |
T29612 |
T29610 |
cc |
or,liver |
R9056 |
T29613 |
T29610 |
conj |
thymus,liver |
R9057 |
T29614 |
T29608 |
prep |
from,skin |
R9058 |
T29615 |
T29616 |
det |
each,genotype |
R9059 |
T29616 |
T29614 |
pobj |
genotype,from |
R9060 |
T29617 |
T29602 |
auxpass |
was,transcribed |
R9061 |
T29618 |
T29602 |
advmod |
reverse,transcribed |
R9062 |
T29619 |
T29602 |
punct |
-,transcribed |
R9063 |
T29620 |
T29602 |
advcl |
using,transcribed |
R9064 |
T29621 |
T29620 |
dobj |
primers,using |
R9065 |
T29622 |
T29623 |
compound |
Apc,F546 |
R9066 |
T29623 |
T29621 |
appos |
F546,primers |
R9067 |
T29624 |
T29623 |
punct |
-,F546 |
R9068 |
T29625 |
T29626 |
punct |
(,TGAGGAATTTGTCTTGGCGAG |
R9069 |
T29626 |
T29623 |
parataxis |
TGAGGAATTTGTCTTGGCGAG,F546 |
R9070 |
T29627 |
T29626 |
punct |
),TGAGGAATTTGTCTTGGCGAG |
R9071 |
T29628 |
T29623 |
cc |
and,F546 |
R9072 |
T29629 |
T29630 |
compound |
Apc,R721 |
R9073 |
T29630 |
T29623 |
conj |
R721,F546 |
R9074 |
T29631 |
T29630 |
punct |
-,R721 |
R9075 |
T29632 |
T29633 |
punct |
(,GCACTTCCCATGGCAATCATT |
R9076 |
T29633 |
T29630 |
parataxis |
GCACTTCCCATGGCAATCATT,R721 |
R9077 |
T29634 |
T29633 |
punct |
),GCACTTCCCATGGCAATCATT |
R9078 |
T29635 |
T29602 |
punct |
", ",transcribed |
R9079 |
T29636 |
T29602 |
advcl |
resulting,transcribed |
R9080 |
T29637 |
T29636 |
prep |
in,resulting |
R9081 |
T29638 |
T29639 |
nummod |
528,bp |
R9082 |
T29639 |
T29641 |
nmod |
bp,products |
R9083 |
T29640 |
T29639 |
punct |
-,bp |
R9084 |
T29641 |
T29637 |
pobj |
products,in |
R9085 |
T29642 |
T29639 |
cc |
and,bp |
R9086 |
T29643 |
T29644 |
nummod |
313,bp |
R9087 |
T29644 |
T29639 |
conj |
bp,bp |
R9088 |
T29645 |
T29644 |
punct |
-,bp |
R9089 |
T29646 |
T29641 |
prep |
from,products |
R9090 |
T29647 |
T29648 |
det |
the,alleles |
R9091 |
T29648 |
T29646 |
pobj |
alleles,from |
R9092 |
T29649 |
T29650 |
amod |
wild,type |
R9093 |
T29650 |
T29648 |
nmod |
type,alleles |
R9094 |
T29651 |
T29650 |
punct |
-,type |
R9095 |
T29652 |
T29650 |
punct |
/,type |
R9096 |
T29653 |
T29650 |
appos |
ApcCKO,type |
R9097 |
T29654 |
T29648 |
cc |
and,alleles |
R9098 |
T29655 |
T29656 |
compound |
ApcΔ580,allele |
R9099 |
T29656 |
T29648 |
conj |
allele,alleles |
R9100 |
T29657 |
T29636 |
punct |
", ",resulting |
R9101 |
T29658 |
T29636 |
advmod |
respectively,resulting |
R9102 |
T29659 |
T29602 |
punct |
.,transcribed |