PMC:1564426 / 54546-55129 JSONTXT

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    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T28744","span":{"begin":0,"end":4},"obj":"UBERON_EXT:zone_of_skin_or_skin_of_body"},{"id":"T28745","span":{"begin":60,"end":67},"obj":"UBERON:0000922"},{"id":"T28746","span":{"begin":109,"end":114},"obj":"CHEBI:75055"},{"id":"T28747","span":{"begin":124,"end":127},"obj":"CHEBI:60004"},{"id":"T28748","span":{"begin":146,"end":151},"obj":"CHEBI:37586"},{"id":"T28749","span":{"begin":160,"end":165},"obj":"CHEBI:6636"},{"id":"T28750","span":{"begin":172,"end":181},"obj":"CHEBI:30060"},{"id":"T28751","span":{"begin":188,"end":197},"obj":"CHEBI:30059"},{"id":"T28752","span":{"begin":211,"end":216},"obj":"CHEBI:75055"},{"id":"T28753","span":{"begin":283,"end":292},"obj":"UBERON:0007376"},{"id":"T28754","span":{"begin":306,"end":314},"obj":"CHEBI:75958"},{"id":"T28755","span":{"begin":326,"end":335},"obj":"UBERON:0007376"},{"id":"T28756","span":{"begin":354,"end":369},"obj":"GO_EXT:0004565"},{"id":"T28757","span":{"begin":384,"end":393},"obj":"GO_MOP_EXT:catalysis"},{"id":"T28758","span":{"begin":444,"end":451},"obj":"UBERON:0000922"},{"id":"T28759","span":{"begin":508,"end":524},"obj":"CHEBI_EXT:paraformaldehye"}],"text":"Skin permeability assay.\nUnfixed and freshly isolated E18.5 embryos were rinsed in PBS and then submerged in X-gal reaction mix at pH 4.5 (100 mM NaPO4, 1.3 mM MgCl2, 3 mM K3Fe(CN)6, 3 mM K4Fe(CN)6, and 1 mg/ml X-gal) at room temperature overnight [36]. At this pH in the absence of epidermal barrier, the solution penetrates epidermis and an endogenous β-galactosidase–like activity catalyzes production of a blue precipitate. After staining, embryos were washed twice in PBS, fixed overnight at 4 °C in 4% paraformaldehyde, and were photographed using a 35 mm Nikon digital camera."}

    craft-ca-core-dev

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    craft-sa-dev

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    2_test

    {"project":"2_test","denotations":[{"id":"17002498-11923212-85593538","span":{"begin":249,"end":251},"obj":"11923212"},{"id":"T84581","span":{"begin":249,"end":251},"obj":"11923212"}],"text":"Skin permeability assay.\nUnfixed and freshly isolated E18.5 embryos were rinsed in PBS and then submerged in X-gal reaction mix at pH 4.5 (100 mM NaPO4, 1.3 mM MgCl2, 3 mM K3Fe(CN)6, 3 mM K4Fe(CN)6, and 1 mg/ml X-gal) at room temperature overnight [36]. At this pH in the absence of epidermal barrier, the solution penetrates epidermis and an endogenous β-galactosidase–like activity catalyzes production of a blue precipitate. After staining, embryos were washed twice in PBS, fixed overnight at 4 °C in 4% paraformaldehyde, and were photographed using a 35 mm Nikon digital camera."}