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genotyped a total of 458 pups (8–10 d old) from 67 litters resulting from crosses between K14-cre; ApcCKO/+ and ApcCKO/CKO mice. The mutant mice of the genotype K14-cre; ApcCKO/CKO (hereafter, KA mutant) were born, but the observed frequency of KA mutants was much less than expected (78 of 458 [17.0%]; p \u003c 0.0005 Chi-square analysis, Table 1). To assess the basis for the neonatal lethality of KA mutants, we monitored three litters from birth to weaning by measuring the body weight of each pup every day. A total of 25 pups were born from three litters, of which 7 (28%) were confirmed to be K14-cre; ApcCKO/CKO by genotyping, indicating that KA mutants were born in the expected Mendelian ratio. The KA mutant pups were nursed normally, and there was milk in their stomachs during the first 2 or 3 d after birth, but they failed to thrive (Figure 2). By postnatal day (P) 8–10, at the time of genotyping, many KA mutant pups were considerably smaller than their littermates (Figure 2B–2F) and some have died at or prior to this age. None of KA mutants survived to weaning age."}