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PMC:1540739 / 9166-9557 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T5812 0-391 sentence denotes We next mutated the C-terminal part of the p53 gene in two rounds of PCR mutagenesis, first with primers 5′-GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC-3′ and 5′-GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC-3′, which removed the stop codon and introduced a BamHI site, then with primers 5′-GACGGATCCCCTCTGAATTCCGTCCCCATCACCA-3′ and 5′-TGGTGATGGGGACGGAATACAGAGGGGATCCGTC-3′, which introduced an EcoRI site.
T5813 1-3 PRP denotes We
T5814 9-16 VBD denotes mutated
T5815 4-8 RB denotes next
T5816 17-20 DT denotes the
T5817 32-36 NN denotes part
T5818 21-22 NN denotes C
T5819 23-31 JJ denotes terminal
T5820 22-23 HYPH denotes -
T5821 37-39 IN denotes of
T5822 40-43 DT denotes the
T5823 48-52 NN denotes gene
T5824 44-47 NN denotes p53
T5825 53-55 IN denotes in
T5826 56-59 CD denotes two
T5827 60-66 NNS denotes rounds
T5828 67-69 IN denotes of
T5829 70-73 NN denotes PCR
T5830 74-85 NN denotes mutagenesis
T5831 85-87 , denotes ,
T5832 87-92 RB denotes first
T5833 93-97 IN denotes with
T5834 98-105 NNS denotes primers
T5835 109-145 NN denotes GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
T5836 106-107 CD denotes 5
T5837 107-108 SYM denotes ′
T5838 108-109 HYPH denotes -
T5839 145-146 HYPH denotes -
T5840 146-147 CD denotes 3
T5841 147-148 SYM denotes ′
T5842 149-152 CC denotes and
T5843 153-154 CD denotes 5
T5844 156-192 NN denotes GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
T5845 154-155 SYM denotes ′
T5846 155-156 HYPH denotes -
T5847 192-193 HYPH denotes -
T5848 193-194 CD denotes 3
T5849 194-195 SYM denotes ′
T5850 195-197 , denotes ,
T5851 197-202 WDT denotes which
T5852 203-210 VBD denotes removed
T5853 211-214 DT denotes the
T5854 220-225 NN denotes codon
T5855 215-219 NN denotes stop
T5856 226-229 CC denotes and
T5857 230-240 VBD denotes introduced
T5858 241-242 DT denotes a
T5859 249-253 NN denotes site
T5860 243-248 NN denotes BamHI
T5861 253-255 , denotes ,
T5862 255-259 RB denotes then
T5863 260-264 IN denotes with
T5864 265-272 NNS denotes primers
T5865 276-310 NN denotes GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
T5866 273-274 CD denotes 5
T5867 274-275 SYM denotes ′
T5868 275-276 HYPH denotes -
T5869 310-311 HYPH denotes -
T5870 311-312 CD denotes 3
T5871 312-313 SYM denotes ′
T5872 314-317 CC denotes and
T5873 318-319 CD denotes 5
T5874 321-355 NN denotes TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
T5875 319-320 SYM denotes ′
T5876 320-321 HYPH denotes -
T5877 355-356 HYPH denotes -
T5878 356-357 CD denotes 3
T5879 357-358 SYM denotes ′
T5880 358-360 , denotes ,
T5881 360-365 WDT denotes which
T5882 366-376 VBD denotes introduced
T5883 377-379 DT denotes an
T5884 386-390 NN denotes site
T5885 380-385 NN denotes EcoRI
T5886 390-391 . denotes .
R1675 T5813 T5814 nsubj We,mutated
R1676 T5815 T5814 advmod next,mutated
R1677 T5816 T5817 det the,part
R1678 T5817 T5814 dobj part,mutated
R1679 T5818 T5819 npadvmod C,terminal
R1680 T5819 T5817 amod terminal,part
R1681 T5820 T5819 punct -,terminal
R1682 T5821 T5817 prep of,part
R1683 T5822 T5823 det the,gene
R1684 T5823 T5821 pobj gene,of
R1685 T5824 T5823 compound p53,gene
R1686 T5825 T5814 prep in,mutated
R1687 T5826 T5827 nummod two,rounds
R1688 T5827 T5825 pobj rounds,in
R1689 T5828 T5827 prep of,rounds
R1690 T5829 T5830 compound PCR,mutagenesis
R1691 T5830 T5828 pobj mutagenesis,of
R1692 T5831 T5814 punct ", ",mutated
R1693 T5832 T5833 advmod first,with
R1694 T5833 T5814 prep with,mutated
R1695 T5834 T5835 nmod primers,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1696 T5835 T5833 pobj GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC,with
R1697 T5836 T5835 nummod 5,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1698 T5837 T5836 punct ′,5
R1699 T5838 T5835 punct -,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1700 T5839 T5835 punct -,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1701 T5840 T5835 nummod 3,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1702 T5841 T5835 punct ′,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1703 T5842 T5835 cc and,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1704 T5843 T5844 nummod 5,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1705 T5844 T5835 conj GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1706 T5845 T5843 punct ′,5
R1707 T5846 T5844 punct -,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1708 T5847 T5844 punct -,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1709 T5848 T5844 nummod 3,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1710 T5849 T5844 punct ′,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1711 T5850 T5835 punct ", ",GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1712 T5851 T5852 dep which,removed
R1713 T5852 T5835 relcl removed,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1714 T5853 T5854 det the,codon
R1715 T5854 T5852 dobj codon,removed
R1716 T5855 T5854 compound stop,codon
R1717 T5856 T5852 cc and,removed
R1718 T5857 T5852 conj introduced,removed
R1719 T5858 T5859 det a,site
R1720 T5859 T5857 dobj site,introduced
R1721 T5860 T5859 compound BamHI,site
R1722 T5861 T5833 punct ", ",with
R1723 T5862 T5863 advmod then,with
R1724 T5863 T5833 prep with,with
R1725 T5864 T5865 nmod primers,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1726 T5865 T5863 pobj GACGGATCCCCTCTGAATTCCGTCCCCATCACCA,with
R1727 T5866 T5865 nummod 5,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1728 T5867 T5866 punct ′,5
R1729 T5868 T5865 punct -,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1730 T5869 T5865 punct -,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1731 T5870 T5865 nummod 3,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1732 T5871 T5865 punct ′,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1733 T5872 T5865 cc and,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1734 T5873 T5874 nummod 5,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1735 T5874 T5865 conj TGGTGATGGGGACGGAATACAGAGGGGATCCGTC,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1736 T5875 T5873 punct ′,5
R1737 T5876 T5874 punct -,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1738 T5877 T5874 punct -,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1739 T5878 T5874 nummod 3,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1740 T5879 T5874 punct ′,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1741 T5880 T5865 punct ", ",GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1742 T5881 T5882 dep which,introduced
R1743 T5882 T5865 relcl introduced,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1744 T5883 T5884 det an,site
R1745 T5884 T5882 dobj site,introduced
R1746 T5885 T5884 compound EcoRI,site
R1747 T5886 T5814 punct .,mutated

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5713 9-16 SO_EXT:sequence_alteration_process denotes mutated
T5714 21-31 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T5715 32-39 _FRAGMENT denotes part of
T5716 48-52 SO_EXT:0000831 denotes gene
T5717 44-47 PR_EXT:000003035 denotes p53
T5718 74-85 SO_EXT:sequence_alteration_process denotes mutagenesis
T5719 98-105 SO_EXT:0000112 denotes primers
T5720 215-225 SO_EXT:0000319 denotes stop codon
T5721 243-248 PR_EXT:P23940 denotes BamHI
T5722 265-272 SO_EXT:0000112 denotes primers
R1617 T5716 T5715 _lexicallyChainedTo gene,part of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5677 32-39 _FRAGMENT denotes part of
T5678 48-52 SO:0000831 denotes gene
T5679 44-47 PR:000003035 denotes p53
T5680 98-105 SO:0000112 denotes primers
T5681 215-225 SO:0000319 denotes stop codon
T5682 243-248 PR:P23940 denotes BamHI
T5683 265-272 SO:0000112 denotes primers
R1616 T5678 T5677 _lexicallyChainedTo gene,part of